Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 1696 279.3 15874187 97.8% 15524954 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,999,913 C→T A516V (GCC→GTC)  b2866 → hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,999,9130CT98.7% 1057.5 / ‑2.0 304A516V (GCC→GTC) b2866hypothetical protein
Reads supporting (aligned to +/- strand):  ref base C (0/4);  new base T (167/133);  total (167/137)
Fisher's exact test for biased strand distribution p-value = 4.03e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.69e-01

Only 100 of 313 total aligned reads displayed.

GGCAGAAACCGTGGGGGTTCCGGTCAGCGACGTTCGCGTTATTTCAACTCAAGATACCGACGTTACGCCGTTCGATCCCGGCGCATTTGCCTCACGCCAGAGCTATGTTGCCGCGCCTGCGCTGCGCAGTGCGGCACTATTATTAAAAGAGAAAATCATCGCTCACGCCGCAGTCATGC  >  NC_000913/2999824‑3000002
                                                                                         |                                                                                         
ggCAGAAACCGTGGGGGTTCCGGTCAGCGACGTTCGCGTTATTTCAACTCAAGATACCGACGTTACGCCGTTCGATCCCGGCGCATTTGtctc                                                                                        <  1:1293385/93‑1 (MQ=255)
ggCAGAAACCGTGGGGGTTCCGGTCAGCGACGTTCGCGTTATTTCAACTCAAGATACCGACGTTACGCCGTTCGATCCCGGCGCATTTGtctc                                                                                        <  1:4970723/93‑1 (MQ=255)
ggCAGAAACCGTGGGGGTTCCGGTCAGCGACGTTCGCGTTATTTCAACTCAAGATACCGACGTTACGCCGTTCGATCCCGGCGCATTTGtctc                                                                                        <  1:3624380/93‑1 (MQ=255)
 gCAGAAGCCGTGGGGGTTCCGGTCAGCGACGTTCGCGTTATTTCAACTCAAGATACCGACGTTACGCCGTTCGATCCCGGCGCATTTGTCTCa                                                                                       >  2:7785287/1‑93 (MQ=255)
 gCAGAAACCGTGGGGGTTCCGGTCAGCGACGTTCGCGTTATTTCAACTCAAGATACCGACGTTACGCCGTTCGATCCCGGCGCATTTGTCTCa                                                                                       <  2:4050262/93‑1 (MQ=255)
 gCAGAAACCGTGGGGGTTCCGGTCAGCGACGTTCGCGTTATTTCAACTCAAGATACCGACGTTACGCCGTTCGATCCCGGCGCATTTGTCTCa                                                                                       >  2:5437517/1‑93 (MQ=255)
 gCAGAAACCGTGGGGGTTCCGGTCAGCGACGTTCGCGTTATTTCAACTCAAGATACCGACGTTACGCCGTTCGATCCCGGCGCATTTGTCTCa                                                                                       >  2:1519957/1‑93 (MQ=255)
       aCCGTGGGGGTTCCGGTCAGCGACGTTCGCGTTATTTCAACTCAAGATACCGACGTTACGCCGTTCGATCCCGGCGCATTTGTCTCACGCCag                                                                                 <  1:6686392/93‑1 (MQ=255)
       aCCGTGGGGGTTCCGGTCAGCGACGTTCGCGTTATTTCAACTCAAGATACCGACGTTACGCCGTTCGATCCCGGCGCATTTGTCTCACGCCag                                                                                 >  2:3401405/1‑93 (MQ=255)
       aCCGTGGGGGTTCCGGTCAGCGACGTTCGCGTTATTTCAACTCAAGATACCGACGTTACGCCGTTCGATCCCGGCGCATTTGTCTCACGCCag                                                                                 <  2:2284452/93‑1 (MQ=255)
           tGGGGGTTCCGGTCAGCGACGTTCGCGTTATTTCAACTCAAGATACCGACGTTACGCCGTTCGATCCCGGCGCATTTGTCTCACGCCAGAGCt                                                                             >  2:4664736/1‑93 (MQ=255)
            gggggTTCCGGTCAGCGACGTTCGCGTTATTTCAACTCAAGATACCGACGTTACGCCGTTCGATCCCGGCGCATTTGTCTCACGCCAGAGCTa                                                                            <  1:2468056/93‑1 (MQ=255)
             ggggTTCCGGTCAGCGACGTTCGCGTTATTTCAACTCAAGATACCGACGTTACGCCGTTCGATCCCGGCGCATTTGTCTCACGCCAGAGCTAt                                                                           <  1:578118/93‑1 (MQ=255)
              gggTTCCGGTCAGCGACGTTCGCGTTATTTCAACTCAAGATACCGACGTTACGCCGTTCGATCCCGGCGCATTTGTCTCACGCCAGAGCTATg                                                                          <  2:1120868/93‑1 (MQ=255)
               ggTTCCGGTCAGCGACGTTCGCGTTATTTCAACTCAAGATACCGACGTTACGCCGTTCGATCCCGGCGCATTTGTCTCACGCCAGAGCTATGt                                                                         <  1:1546222/93‑1 (MQ=255)
                   ccGGTCAGCGACGTTCGCGTTATTTCAACTCAAGATACCGACGTTACGCCGTTCGATCCCGGCGCATTTGTCTCACGCCAGAGCTATGTTGcc                                                                     >  2:1824671/1‑93 (MQ=255)
                   ccGGTCAGCGACGTTCGCGTTATTTCAACTCAAGATACCGACGTTACGCCGTTCGATCCCGGCGCATTTGTCTCACGCCAGAGCTATGTTGcc                                                                     <  1:7586255/93‑1 (MQ=255)
                   ccGGTCAGCGACGTTCGCGTTATTTCAACTCAAGATACCGACGTTACGCCGTTCGATCCCGGCGCATTTGTCTCACGCCAGAGCTATGTTGcc                                                                     >  2:2904952/1‑93 (MQ=255)
                   ccGGTCAGCGACGTTCGCGTTATTTCAACTCAAGATACCGACGTTACGCCGTTCGATCCCGGCGCATTTGTCTCACGCCAGAGCTATGTTGcc                                                                     <  2:4310969/93‑1 (MQ=255)
                     ggTCAGCGACGTTCGCGTTATTTCAACTCAAGATACCGACGTTACGCCGTTCGATCCCGGCGCATTTGTCTCACGCCAGAGCTATGTTGCcgc                                                                   >  2:2633278/1‑93 (MQ=255)
                     ggTCAGCGACGTTCGCGTTATTTCAACTCAAGATACCGACGTTACGCCGTTCGATCCCGGCGCATTTGTCTCACGCCAGAGCTATGTTGCcgc                                                                   >  2:6315635/1‑93 (MQ=255)
                      gTCAGCGACGTTCGCGTTATTTCAACTCAAGATACCGACGTTACGCCGTTCGATCCCGGCGCATTTGTCTCACGCCAGAGCTATGTTGCcgcg                                                                  >  1:2140342/1‑93 (MQ=255)
                       tCAGCGACGTTCGCGTTATTTCAACTCAAGATACCGACGTTACGCCGTTCGATCCCGGCGCATTTGTCTCACGCCAGAGCTATGTTGCCgcgc                                                                 >  1:5514351/1‑93 (MQ=255)
                        cAGCGACGTTCGCGTTATTTCAACTCAAGATACCGACGTTACGCCGTTCGATCCCGGCGCATTTGTCTCACGCCAGAGCTATGTTGCCGCGcc                                                                >  1:3224835/1‑93 (MQ=255)
                         aGCGACGTTCGCGTTATTTCAACTCAAGATACCGACGTTACGCCGTTCGATCCCGGCGCATTTGTCTCACGCCAGAGCTATGTTGCCGCGCct                                                               >  2:1966106/1‑93 (MQ=255)
                         aGCGACGTTCGCGTTATTTCAACTCAAGATACCGACGTTACGCCGTTCGATCCCGGCGCATTTGTCTCACGCCAGAGCTATGTTGCCGCGCct                                                               >  1:5601636/1‑93 (MQ=255)
                            gACGTTCGCGTTATTTCAACTCAAGATACCGACGTTACGCCGTTCGATCCCGGCGCATTTGTCTCACGCCAGAGCTATGTTGCCGCGCctgcg                                                            >  1:5546225/1‑93 (MQ=255)
                             aCGTTCGCGTTATTTCAACTCAAGATACCGACGTTACGCCGTTCGATCCCGGCGCATTTGTCTCACGCCAGAGCTATGTTGCCGCGCctgcgc                                                           <  1:3182863/93‑1 (MQ=255)
                              cGTTCGCGTTATTTCAACTCAAGATACCGACGTTACGCCGTTCGATCCCGGCGCATTTGTCTCACGCCAGAGCTATGTTGCCGCGCctgcgct                                                          <  2:6489884/93‑1 (MQ=255)
                               gTTCGCGTTATTTCAACTCAAGATACCGACGTTACGCCGTTCGATCCCGGCGCATTTGTCTCACGCCAGAGCTATGTTGCCGCGCctgcgctg                                                         >  1:535937/1‑93 (MQ=255)
                                ttCGCGTTATTTCAACTCAAGATACCGACGTTACGCCGTTCGATCCCGGCGCATTTGTCTCACGCCAGAGCTATGTTGCCGCGCctgcgctgc                                                        >  2:5749105/1‑93 (MQ=255)
                                ttCGCGTTATTTCAACTCAAGATACCGACGTTACGCCGTTCGATCCCGGCGCATTTGTCTCACGCCAGAGCTATGTTGCCGCGCctgcgctgc                                                        <  1:6364157/93‑1 (MQ=255)
                                ttCGCGTTATTTCAACTCAAGATACCGACGTTACGCCGTTCGATCCCGGCGCATTTGTCTCACGCCAGAGCTATGTTGCCGCGCctgcgctgc                                                        <  1:3442975/93‑1 (MQ=255)
                                ttCGCGTTATTTCAACTCAAGATACCGACGTTACGCCGTTCGATCCCGGCGCATTTGTCTCACGCCAGAGCTATGTTGCCGCGCctgcgctgc                                                        >  1:7268896/1‑93 (MQ=255)
                                ttCGCGTTATTTCAACTCAAGATACCGACGTTACGCCGTTCGATCACGGCGCATTTGTCTCACGCCAGAGCTATGTTGCCGCGCctgcgctgc                                                        >  2:5800478/1‑93 (MQ=255)
                                gtCGCGTTATTTCAACTCAAGATACCGACGTTACGCCGTTCGATCCCGGCGCATTTGTCTCACGCCAGAGCTAGGTTGCCGCGCctgcgctgc                                                        <  2:5290161/92‑1 (MQ=255)
                                  cgcgTTATTTCAACTCAAGATACCGACGTTACGCCGTTCGATCCCGGCGCATTTGTCTCACGCCAGAGCTATGTTGCCGCGCCtgcgctgcgc                                                      <  1:6451483/93‑1 (MQ=255)
                                   gcgTTATTTCAACTCAAGATACCGACGTTACGCCGTTCGATCCCGGCGCATTTGTCTCACGCCAGAGCTATGTTGCCGCGCCTGCGCTGCGCa                                                     >  2:4093687/1‑93 (MQ=255)
                                    cgTTATTTCAACTCAAGATACCGACGTTACGCCGTTCGATCCCGGCGCATTTGTCTCACGCCAGAGCTATGTTGCCGCGCCTGCGCTGCGCAg                                                    >  1:1672139/1‑93 (MQ=255)
                                     gTTATTTCAACTCAAGATACCGACGTTACGCCGTTCGATCCCGGCGCATTTGTCTCACGCCAGAGCTATGTTGCCGCGCCTGCGCTGCGCAGt                                                   >  2:7718118/1‑93 (MQ=255)
                                     gTTATTTCAACTCAAGATACCGACGTTACGCCGTTCGATCCCGGCGCATTTGTCTCACGCCAGAGCTATGTTGCCGCGCCTGCGCTGCGCAGt                                                   >  1:2744300/1‑93 (MQ=255)
                                     gTTATTTCAACTCAAGATACCGACGTTACGCCGTTCGATCCCGGCGCATTTGTCTCACGCCAGAGCTATGTTGCCGCGCCTGCGCTGCGCAGt                                                   >  1:1790137/1‑93 (MQ=255)
                                      ttATTTCAACTCAAGATACCGACGTTACGCCGTTCGATCCCGGCGCATTTGTCTCACGCCAGAGCTATGTTGCCGCGCCTGCGCTGCGCAGTg                                                  <  2:7612684/93‑1 (MQ=255)
                                      ttATTTCAACTCAAGATACCGACGTTACGCCGTTCGATCCCGGCGCATTTGTCTCACGCCAGAGCTATGTTGCCGCGCCTGCGCTGCGCAGTg                                                  >  2:6251482/1‑93 (MQ=255)
                                       tATTTCAACTCAAGATACCGACGTTACGCCGTTCGATCCCGGCGCATTTGTCTCACGCCAGAGCTATGTTGCCGCGCCTGCGCTGCGCAGTGc                                                 <  2:515971/93‑1 (MQ=255)
                                       tATTTCAACTCAAGATACCGACGTTACGCCGTTCGATCCCGGCGCATTTGTCTCACGCCAGAGCTATGTTGCCGCGCCTGCGCTGCGCAGTGc                                                 >  1:4753446/1‑93 (MQ=255)
                                       tATTTCAACTCAAGATACCGACGTTACGCCGTTCGATCCCGGCGCATTTGTCTCACGCCAGAGCTATGTTGCCGCGCCTGCGCTGCGCAGTGc                                                 <  2:2690727/93‑1 (MQ=255)
                                       tATTTCAACTCAAGATACCGACGTTACGCCGTTCGATCCCGGCGCATTTGTCTCACGCCAGAGCTATGTTGCCGCGCCTGCGCTGCGCAGTGc                                                 <  2:3175634/93‑1 (MQ=255)
                                       tATTTCAACTCAAGATACCGACGTTACGCCGTTCGATCCCGGCGCATTTGTCTCACGCCAGAGCTATGTTGCCGCGCCTGCGCTGCGCAGTGc                                                 <  2:3984525/93‑1 (MQ=255)
                                       tATTTCAACTCAAGATACCGACGTTACGCCGTTCGATCCCGGCGCATTTGTCTCACGCCAGAGCTATGTTGCCGCGCCTGCGCTGCGCAGTGc                                                 >  1:905099/1‑93 (MQ=255)
                                       tATTTCAACTCAAGATACCGACGTTACGCCGTTCGATCCCGGCGCATTTGTCTCACGCCAGAGCTATGTTGCCGCGCCTGCGCTGCGCAGTGc                                                 >  1:3003774/1‑93 (MQ=255)
                                           tCAACTCAAGATACCGACGTTACGCCGTTCGATCCCGGCGCATTTGTCTCACGCCAGAGCTATGTTGCCGCGCCTGCGCTGCGCAGTGCGGCa                                             >  1:60740/1‑93 (MQ=255)
                                            cAACTCAAGATACCGACGTTACGCCGTTCGATCCCGGCGCATTTGTCTCACGCCAGAGCTATGTTGCCGCGCCTGCGCTGCGCAGTGCGGCAc                                            <  1:6817483/93‑1 (MQ=255)
                                              aCTCAAGATACCGACGTTACGCCGTTCGATCCCGGCGCATTTGTCTCACGCCAGAGCTATGTTGCCGCGCCTGCGCTGCGCAGTGCGGCACta                                          >  1:5051372/1‑93 (MQ=255)
                                               cTCAAGATACCGACGTTACGCCGTTCGATCCCGGCGCATTTGTCTCACGCCAGAGCTATGTTGCCGCGCCTGCGCTGCGCAGTGCGGCACtat                                         <  1:7894699/93‑1 (MQ=255)
                                               cTCAAGATACCGACGTTACGCCGTTCGATCCCGGCGCATTTGTCTCACGCCAGAGCTATGTTGCCGCGCCTGCGCTGCGCAGTGCGGCACtat                                         <  2:6862038/93‑1 (MQ=255)
                                                tCAAGATACCGACGTTACGCCGTTCGATCCCGGCGCATTTGTCTCACGCCAGAGCTATGTTGCCGCGCCTGCGCTGCGCAGTGCGGCACtatt                                        <  2:4514090/93‑1 (MQ=255)
                                                tCAAGATACCGACGTTACGCCGTTCGATCCCGGCGCATTTGTCTCACGCCAGAGCTATGTTGCCGCGCCTGCGCTGCGCAGTGCGGCACtatt                                        >  1:5141132/1‑93 (MQ=255)
                                                tCAAGATACCGACGTTACGCCGTTCGATCCCGGCGCATTTGTCTCACGCCAGAGCTATGTTGCCGCGCCTGCGCTGCGCAGTGCGGCACtatt                                        >  2:4957543/1‑93 (MQ=255)
                                                 cAAGATACCGACGTTACGCCGTTCGATCCCGGCGCATTTGTCTCACGCCAGAGCTATGTTGCCGCGCCTGCGCTGCGCAGTGCGGCACtatta                                       <  1:111287/93‑1 (MQ=255)
                                                  aaGATACGGACGTTACGCCGTTCGATCCCGGCGCATTTGTCTCACGCCAGAGCTATGTTGCCGCGCCTGCGCTGCGCAGTGCGGCACtattat                                      <  2:7094916/93‑1 (MQ=255)
                                                  aaGATACCGACGTTACGCCGTTCGATCCCGGCGCATTTGTCTCACGCCAGAGCTATGTTGCCGCGCCTGCGCTGCGCAGTGCGGCACtattat                                      >  2:5339072/1‑93 (MQ=255)
                                                  aaGATACCGACGTTACGCCGTTCGATCCCGGCGCATTTGTCTCACGCCAGAGCTATGTTGCCGCGCCTGCGCTGCGCAGTGCGGCACtattat                                      >  2:2100594/1‑93 (MQ=255)
                                                  aaGATACCGACGTTACGCCGTTCGATCCCGGCGCATTTGTCTCACGCCAGAGCTATGTTGCCGCGCCTGCGCTGCGCAGTGCGGCACtattat                                      >  1:7612930/1‑93 (MQ=255)
                                                    gATACCGACGTTACGCCGTTCGATCCCGGCGCATTTGTCTCACGCCAGAGCTATGTTGCCGCGCCTGCGCTGCGCAGTGCGGCACTAttatta                                    >  1:5321209/1‑93 (MQ=255)
                                                      tACCGACGTTACGCCGTTCGATCCCGGCGCATTTGTCTCACGCCAGAGCTATGTTGCCGCGCCTGCGCTGCGCAGTGCGGCACTATTATTaaa                                  >  2:1253229/1‑93 (MQ=255)
                                                      tACCGACGTTACGCCGTTCGATCCCGGCGCATTTGTCTCACGCCAGAGCTATGTTGCCGCGCCTGCGCTGCGCAGTGCGGCACTATTATTaaa                                  <  1:7107656/93‑1 (MQ=255)
                                                         cGACGTTACGCCGTTCGATCCCGGCGCATTTGTCTCACGCCAGAGCTATGTTGCCGCGCCTGCGCTGCGCAGTGCGGCACTATTATTAAAaga                               <  1:3787129/93‑1 (MQ=255)
                                                         cGACGTTACGCCGTTCGATCCCGGCGCATTTGTCTCACGCCAGAGCTATGTTGCCGCGCCTGCGCTGCGCAGTGCGGCACTATTATTAAAaga                               >  2:4781183/1‑93 (MQ=255)
                                                           aCGTTACGCCGTTCGATCCCGGCGCATTTGTCTCACGCCAGAGCTATGTTGCCGCGCCTGCGCTGCGCAGTGCGGCACTATTATTAAAAgaga                             >  1:6174653/1‑93 (MQ=255)
                                                             gTTACGCCGTTCGATCCCGGCGCATTTGTCTCACGCCAGAGCTATGTTGCCGCGCCTGCGCTGCGCAGTGCGGCACTATTATTAAAAGAGaaa                           >  2:3632378/1‑93 (MQ=255)
                                                             gTTACGCCGTTCGATCCCGGCGCATTTGTCTCACGCCAGAGCTATGTTGCCGCGCCTGCGCTGCGCAGTGCGGCACTATTATTAAAAGAGaaa                           <  1:972947/93‑1 (MQ=255)
                                                              ttACGCCGTTCGATCCCGGCGCATTTGTCTCACGCCAGAGCTATGTTGCCGCGCCTGCGCTGCGCAGTGCGGCACTATTATTAAAAGAGaaaa                          >  2:1614694/1‑93 (MQ=255)
                                                                 cGCCGTTCGATCCCGGCGCATTTGTCGCACGCCAGAGCTATGTTGCCGCGCCTGCGCTGCGCAGTGCGGCACTATTATTAAAAGAGAAAatca                       <  1:3904712/93‑1 (MQ=255)
                                                                  gCCGTTCGATCCCGGCGCATTTGTCTCACGCCAGAGCTATGTTGCCGCGCCTGCGCTGCGCAGTGCGGCACTATTATTAAAAGAGAAAatcat                      <  1:2013202/93‑1 (MQ=255)
                                                                   ccGTTCGATCCCGGCGCATTTGTCTCACGCCAGAGCTATGTTGCCGCGCCTGCGCTGCGCAGTGCGGCACTATTATTAAAAGAGAAAatcatc                     <  2:3825543/93‑1 (MQ=255)
                                                                   ccGTTCGATCCCGGCGCATTTGTCTCACGCCAGAGCTATGTTGCCGCGCCTGCGCTGCGCAGTGCGGCACTATTATTAAAAGAGAAAatcatc                     >  2:6039953/1‑93 (MQ=255)
                                                                     gTTCGATCCCGGCGCATTTGTCTCACGCCAGAGCTATGTTGCCGCGCCTGCGCTGCGCAGTGCGGCACTATTATTAAAAGAGAAAATCATCGc                   >  2:7880748/1‑93 (MQ=255)
                                                                     gTTCGATCCCGGCGCATTTGTCTCACGCCAGAGCTATGTTGCCGCGCCTGCGCTGCGCAGTGCGGCACTATTATTAAAAGAGAAAATCATCGc                   >  1:4027525/1‑93 (MQ=255)
                                                                      ttCGATCCCGGCGCATTTGTCTCACGCCAGAGCTATGTTGCCGCGCCTGCGCTGCGCAGTGCGGCACTATTATTAAAAGAGAAAATCATCGCt                  >  2:5650523/1‑93 (MQ=255)
                                                                      ttCGATCCCGGCGCATTTGTCTCACGCCAGAGCTATGTTGCCGCGCCTGCGCTGCGCAGTGCGGCACTATTATTAAAAGAGAAAATCATCGCt                  >  1:2606066/1‑93 (MQ=255)
                                                                        cGATCCCGGCGCATTTGTCTCACGCCAGAGCTATGTTGCCGCGCCTGCGCTGCGCAGTGCGGCACTATTATTAAAAGAGAAAATCATCGCTCa                <  2:803857/93‑1 (MQ=255)
                                                                        cGATCCCGGCGCATTTGTCTCACGCCAGAGCTATGTTGCCGCGCCTGCGCTGCGCAGTGCGGCACTATTATTAAAAGAGAAAATCATCGCTCa                <  2:6607933/93‑1 (MQ=255)
                                                                        cGATCCCGGCGCATTTGTCTCACGCCAGAGCTATGTTGCCGCGCCTGCGCTGCGCAGTGCGGCACTATTATTAAAAGAGAAAATCATCGCTCa                <  1:877112/93‑1 (MQ=255)
                                                                        cGATCCCGGCGCATTTGTCTCACGCCAGAGCTATGTTGCCGCGCCTGCGCTGCGCAGTGCGGCACTATTATTAAAAGAGAAAATCATCGCTCa                <  1:676323/93‑1 (MQ=255)
                                                                         gATCCCGGCGCATTTGTCTCACGCCAGAGCTATGTTGCCGCGCCTGCGCTGCGCAGTGCGGCACTATTATTAAAAGAGAAAATCATCGCTCAc               <  2:2007315/93‑1 (MQ=255)
                                                                           tCCCGGCGCATTTGTCTCACGCCAGAGCTATGTTGCCGCGCCTGCGCTGCGCAGTGCGGCACTATTATTAAAAGAGAAAATCATCGCTCAcgc             >  2:3901116/1‑93 (MQ=255)
                                                                            cccGGCGCATTTGTCTCACGCCAGAGCTATGTTGCCGCGCCTGCGCTGCGCAGTGCGGCACTATTATTAAAAGAGAAAATCATCGCNCAcgcc            >  1:4291517/1‑93 (MQ=255)
                                                                             ccGGCGCATTTGTCTCACGCCAGAGCTATGTTGCCGCGCCTGCGCTGCGCAGTGCGGCACTATTATTAAAAGAGAAAATCATCGCTCAcgccg           >  1:547462/1‑93 (MQ=255)
                                                                             ccGACGCATTTGTCTCACGCCAGAGCTATGTTGCCGCGCCTGCGCTGCGCAGTGCGGCACTATTATTAAAAGAGAAAATCATCGCTCAcgccg           >  2:7541041/1‑93 (MQ=255)
                                                                              cGGCGCATTTGTCTCACGCCAGAGCTATGTTGCCGCGCCTGCGCTGCGCAGTGCGGCACTATTATTAAAAGAGAAAATCATCGCTCAcgccgc          >  2:6153208/1‑93 (MQ=255)
                                                                               ggCGCATTTGTCTCACGCCAGAGCTATGTTGCCGCGCCTGCGCTGCGCAGTGCGGCACTATTATTAAAAGAGAAAATCATCGCTCACGCCGCa         >  2:7240882/1‑93 (MQ=255)
                                                                               ggCGCATTTGTCTCACGCCAGAGCTATGTTGCCGCGCCTGCGCTGCGCAGTGCGGCACTATTATTAAAAGAGAAAATCATCGCTCACGCCGCa         <  2:7415437/93‑1 (MQ=255)
                                                                               ggCGCATTTGTCTCACGCCAGAGCTATGTTGCCGCGCCTGCGCTGCGCAGTGCGGCACTATTATTAAAAGAGAAAATCATCGCTCACGCCGCa         <  2:3247870/93‑1 (MQ=255)
                                                                                   cATTTGTCTCACGCCAGAGCTATGTTGCCGCGCCTGCGCTGCGCAGTGCGGCACTATTATTAAAAGAGAAAATCATCGCTCACGCCGCAGTCa     >  2:6753255/1‑93 (MQ=255)
                                                                                    aTTTGTCTCACGCCAGAGCTATGTTGCCGCGCCTGCGCTGCGCAGTGCGGCACTATTATTAAAAGAGAAAATCATCGCTCACGCCGCAGTCAt    >  2:6816535/1‑93 (MQ=255)
                                                                                     tttGTCTCACGCCAGAGCTATGTTGCCGCGCCTGCGCTGCGCAGTGCGGCACTATTATTAAAAGAGAAAATCATCGCTCACGCCGCAGTCATg   >  2:5955931/1‑93 (MQ=255)
                                                                                     tttGTCTCACGCCAGAGCTATGTTGCCGCGCCTGCGCTGCGCAGTGCGGCACTATTATTAAAAGAGAAAATCATCGCTCACGCCGCAGTCATg   <  2:5692480/93‑1 (MQ=255)
                                                                                     tttGTCTCACGCCAGAGCTATGTTGCCGCGCCTGCGCTGCGCAGTGCGGCACTATTATTAAAAGAGAAAATCATCGCTCACGCCGCAGTCATg   >  1:5894100/1‑93 (MQ=255)
                                                                                      ttGTCTCACGCCAGAGCTATGTTGCCGCGCCTGCGCTGCGCAGTGCGGCACTATTATTAAAAGAGAAAATCATCGCTCACGCCGCAGTCATGc  <  1:4586261/93‑1 (MQ=255)
                                                                                         |                                                                                         
GGCAGAAACCGTGGGGGTTCCGGTCAGCGACGTTCGCGTTATTTCAACTCAAGATACCGACGTTACGCCGTTCGATCCCGGCGCATTTGCCTCACGCCAGAGCTATGTTGCCGCGCCTGCGCTGCGCAGTGCGGCACTATTATTAAAAGAGAAAATCATCGCTCACGCCGCAGTCATGC  >  NC_000913/2999824‑3000002

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GTGGCAGAAACCGTGGGGGTTCCGGTCAGCGACGTTCGCGTTATTTCAACTCAAGATACCGACGTTACGCCGTTCGATCCCGGCGCATTTGCCTCACGCCAGAGCTATGTTGCCGCGCCTGCGCTGCGCAGTGCGGCACTATTATTAAAAGAGAAAATCATCGCTCACGCCGCAGTCATGCTAC  >  NC_000913/2999822‑3000005
                                                                                           |                                                                                            
GTGGCAGAAACCGTGGGGGTTCCGGTCAGCGACGTTCGCGTTATTTCAACTCAAGATACCGACGTTACGCCGTTCGATCCCGGCGCATTTGTCT                                                                                            <  SRR4302543.4861255/94‑1 (MQ=60)
  GGCAGAAACCGTGGGGGTTCCGGTCAGCGACGTTCGCGTTATTTCAACTCAAGATACCGACGTTACGCCGTTCGATCCCGGCGCATTTGTCTCA                                                                                          <  SRR4302543.3656310/94‑1 (MQ=60)
  GGCAGAAACCGTGGGGGTTCCGGTCAGCGACGTTCGCGTTATTTCAACTCAAGATACCGACGTTACGCCGTTCGATCCCGGCGCATTTGTCTCA                                                                                          <  SRR4302543.1304900/94‑1 (MQ=60)
  GGCAGAAACCGTGGGGGTTCCGGTCAGCGACGTTCGCGTTATTTCAACTCAAGATACCGACGTTACGCCGTTCGATCCCGGCGCATTTGTCTCA                                                                                          <  SRR4302543.5015960/94‑1 (MQ=60)
  GGCAGAAACCGTGGGGGTTCCGGTCAGCGACGTTCGCGTTATTTCAACTCAAGATACCGACGTTACGCCGTTCGATCCCGGCGCATTTGTCTCA                                                                                          >  SRR4302543.7664286/1‑94 (MQ=60)
       AAACCGTGGGGGTTCCGGTCAGCGACGTTCGCGTTATTTCAACTCAAGATACCGACGTTACGCCGTTCGATCCCGGCGCATTTGTCTCACGCCA                                                                                     <  SRR4302543.3723314/94‑1 (MQ=60)
       AAACCGTGGGGGTTCCGGTCAGCGACGTTCGCATTATTTCAACTCAAGATACCGACGTTACGCCGTTCGATCCCGGCGCATTTGTCTCACGCCA                                                                                     <  SRR4302543.2072925/94‑1 (MQ=60)
        AACCGTGGGGGTTCCGGTCAGCGACGTTCGCGTTATTTCAACTCAAGATACCGACGTTACGCCGTTCGATCCCGGCGCATTTGTCTCACGCCAG                                                                                    <  SRR4302543.3559884/94‑1 (MQ=60)
         ACCGTGGGGGTTCCGGTCAGCGACGTTCGCGTTATTTCAACTCAAGATACCGACGTTACGCCGTTCGATCCCGGCGCATTTGTCTCACGCCAGA                                                                                   <  SRR4302543.6749514/94‑1 (MQ=60)
          CCGTGGGGGTTCCGGTCAGCGACGTTCGCGTTATTTCAACTCAAGATACCGACGTTACGCCGTTCGATCCCGGCGCATTTGTCTCACGCCAGAG                                                                                  <  SRR4302543.6847028/94‑1 (MQ=60)
            GTGGGGGTTCCGGTCAGCGACGTTCGCGTTATTTCAACTCAAGATACCGACGTTACGCCGTTCGATCCCGGCGCATTTGTCTCACGCCAGAGCT                                                                                >  SRR4302543.7117245/1‑94 (MQ=60)
            GTGGGGGTTCCGGTCAGCGACGTTCGCGTTATTTCAACTCAAGATACCGACGTTACGCCGTTCGATCCCGGCGCATTTGTCTCACGCCAGAGCT                                                                                <  SRR4302543.5848423/94‑1 (MQ=60)
            GTGGGGGTTCCGGTCAGCGACGTTCGCGTTATTTCAACTCAAGATACCGACGTTACGCCGTTCGATCCCGGCGCATTTGTCTCACGCCAGAGCT                                                                                <  SRR4302543.2039156/94‑1 (MQ=60)
              GGGGGTTCCGGTCAGCGACGTTCGCGTTATTTCAACTCAAGATACCGACGTTACGCCGTTCGATCCCGGCGCATTTGTCTCACGCCAGAGCTAT                                                                              <  SRR4302543.2490332/94‑1 (MQ=60)
               GGGGTTCCGGTCAGCGACGTTCGCGTTATTTCAACTCAAGATACCGACGTTACGCCGTTCGATCCCGGCGCATTTGTCTCACGCCAGAGCTATG                                                                             <  SRR4302543.583407/94‑1 (MQ=60)
               GGGGTTCCGGTCAGCGACGTTCGCGTTATTTCAACTCAAGATACCGACGTTACGCCGTTCGATCCCGGCGCATTTGTCTCACGCCAGAGCTATG                                                                             <  SRR4302543.4869934/94‑1 (MQ=60)
                GGGTTCCGGTCAGCGACGTTCGCGTTATTTCAACTCAAGATACCGACGTTACGCCGTTCGATCCCGGCGCATTTGTCTCACGCCAGAGCTATGT                                                                            >  SRR4302543.7979132/1‑94 (MQ=60)
                 GGTTCCGGTCAGCGACGTTCGCGTTATTTCAACTCAAGATACCGACGTTACGCCGTTCGATCCCGGCGCATTTGTCTCACGCCAGAGCTATGTT                                                                           <  SRR4302543.1963374/94‑1 (MQ=60)
                 GGTTCCGGTCAGCGACGTTCGCGTTATTTCAACTCAAGATACCGACGTTACGCCGTTCGATCCCGGCGCATTTGTCTCACGCCAGAGCTATGTT                                                                           <  SRR4302543.1560066/94‑1 (MQ=60)
                  GTTCCGGTCAGCGACGTTCGCGTTATTTCAACTCAAGATACCGACGTTACGCCGTTCGATCCCGGCGCATTTGTCTCACGCCAGAGCTATGTTG                                                                          >  SRR4302543.1577721/1‑94 (MQ=60)
                    TCCGGTCAGCGACGTTCGCGTTATTTCAACTCAAGATACCGACGTTACGCCGTTCGATCCCGGCGCATTTGTCTCACGCCAGAGCTATGTTGCC                                                                        <  SRR4302543.5644201/94‑1 (MQ=60)
                     CCGGTCAGCGACGTTCGCGTTATTTCAACTCAAGATACCGACGTTACGCCGTTCGATCCCGGCGCATTTGTCTCACGCCAGAGCTATGTTGCCG                                                                       <  SRR4302543.7658346/94‑1 (MQ=60)
                      CGGTCAGCGACGTTCGCGTTATTTCAACTCAAGATACCGACGTTACGCCGTTCGATCCCGGCGCATTTGTCTCACGCCAGAGCTATGTTGCCGC                                                                      >  SRR4302543.407483/1‑94 (MQ=60)
                      CGGTCAGCGACGTTCGCGTTATTTCAACTCAAGATACCGACGTTACGCCGTTCGATCCCGGCGCATTTGTCTCACGCCAGAGCTATGTTGCCGC                                                                      >  SRR4302543.4229098/1‑94 (MQ=60)
                       GGTCAGCGACGTTCGCGTTATTTCAACTCAAGATACCGACGTTACGCCGTTCGATCCCGGCGCATTTGTCTCACGCCAGAGCTATGTTGCCGCG                                                                     >  SRR4302543.2159495/1‑94 (MQ=60)
                       GGTCAGCGACGTTCGCGTTATTTCAACTCAAGATACCGACGTTACGCCGTTCGATCCCGGCGCATTTGTCTCACGCCAGAGCTATGTTGCCGCG                                                                     >  SRR4302543.1721865/1‑94 (MQ=60)
                       GGTCAGCGACGTTCGCGTTATTTCAACTCAAGATACCGACGTTACGCCGTTCGATCCCGGCGCATTTGTCTCACGCCAGAGCTATGTTGCCGCG                                                                     >  SRR4302543.6843342/1‑94 (MQ=60)
                        GTCAGCGACGTTCGCGTTATTTCAACTCAAGATACCGACGTTACGCCGTTCGATCCCGGCGCATTTGTCTCACGCCAGAGCTATGTTGCCGCGC                                                                    >  SRR4302543.5564433/1‑94 (MQ=60)
                         TCAGCGACGTTCGCGTTATTTCAACTCAAGATACCGACGTTACGCCGTTCGATCCCGGCGCATTTGTCTCACGCCAGAGCTATGTTGCCGCGCC                                                                   >  SRR4302543.3253983/1‑94 (MQ=60)
                          CAGCGCCGTTCGCGTTATTTCAACTCAAGATACCGACGTTCCGCCGTTCGATCCCGGCGCATTTGCCTCACGCCAGAGCTATGTTGCCGCGCCT                                                                  <  SRR4302543.393292/94‑1 (MQ=60)
                          CAGCGACGTTCGCGTTATTTCAACTCAAGATACCGACGTTACGCCGTTCGATCCCGGCGCATTTGTCTCACGCCAGAGCTATGTTGCCGCGCCT                                                                  >  SRR4302543.5652677/1‑94 (MQ=60)
                          CAGCGACGTTCGCGTTATTTCAACTCAAGATACCGACGTTACGCCGTTCGATCCCGGCGCATTTGTCTCACGCCAGAGCTATGTTGCCGCGCCT                                                                  <  SRR4302543.4484176/94‑1 (MQ=60)
                           AGCGACGTTCGCGTTATTTCAACTCAAGATACCGACGTTACGCCGTTCGATCCCGGCGCATTTGTCTCACGCCAGAGCTATGTTGCCGCGCCTG                                                                 >  SRR4302543.1894642/1‑94 (MQ=60)
                            GCGACGTTCGCGTTATTTCAACTCAAGATACCGACGTTACGCCGTTCGATCCCGGCGCATTTGTCTCACGCCAGAGCTATGTTGCCGCGCCTGC                                                                <  SRR4302543.6295081/94‑1 (MQ=60)
                            GCGACGTTCGCGTTATTTCAACTCAAGATACCGACGTTACGCCGTTCGATCCCGGCGCATTTGCCTCACGCCAGAGCTATGTTGCCGCGCCTGC                                                                <  SRR4302543.5142196/94‑1 (MQ=60)
                             CGACGTTCGCGTTATTTCAACTCAAGATACCGACGTTACGCCGTTCGATCCCGGCGCATTTGTCTCACGCCAGAGCTATGTTGCCGCGCCTGCG                                                               >  SRR4302543.5596659/1‑94 (MQ=60)
                             CGACGTTCGCGTTATTTCAACTCAAGATACCGACGTTACGCCGTTCGATCCCGGCGCATTTGTCTCACGCCAGAGCTATGTTGCCGCGCCTGCG                                                               >  SRR4302543.3663717/1‑94 (MQ=60)
                               ACGTTCGCGTTATTTCAACTCAAGATACCGACGTTACGCCGTTCGATCCCGGCGCATTTGTCTCACGCCAGAGCTATGTTGCCGCGCCTGCGCT                                                             <  SRR4302543.3211564/94‑1 (MQ=60)
                               ACGTTCGCGTTATTTCAACTCAAGATACCGACGTTACGCCGTTCGATCCCGGCGCATTTGTCTCACGCCAGAGCTATGTTGCCGCGCCTGCGCT                                                             <  SRR4302543.404568/94‑1 (MQ=60)
                                CGTTCGCGTTATTTCAACTCAAGATACCGACGTTACGCCGTTCGATCCCGGCGCATTTGTCTCACGCCAGAGCTATGTTGCCGCGCCTGCGCTG                                                            >  SRR4302543.540905/1‑94 (MQ=60)
                                 GTTCGCGTTATTTCAACTCAAGATACCGACGTTACGCCGTTCGATCCCGGCGCATTTGTCTCACGCCAGAGCTATGTTGCCGCGCCTGCGCTGC                                                           <  SRR4302543.7451545/94‑1 (MQ=60)
                                 GTTCGCGTTATTTCAACTCAAGATACCGACGTTACGCCGTTCGATCCCGGCGCATTTGTCTCACGCCAGAGCTATGTTGCCGCGCCTGCGCTGC                                                           >  SRR4302543.7339122/1‑94 (MQ=60)
                                  TTCGCGTTATTTCAACTCAAGATACCGACGTTACGCCGTTCGATCCCGGCGCATTTGTCTCACGCCAGAGCTATGTTGCCGCGCCTGCGCTGCG                                                          <  SRR4302543.6424001/94‑1 (MQ=60)
                                  TTCGCGTTATTTCAACTCAAGATACCGACGTTACGCCGTTCGATCCCGGCGCATTTGTCTCACGCCAGAGCTATGTTGCCGCGCCTGCGCTGCG                                                          <  SRR4302543.3473802/94‑1 (MQ=60)
                                  TTCGCGTTATTTCAACTCAAGATACCGACGTTACGCCGTTCGATCCCGGCGCATTTGTCTCACGCCAGAGCTATGTTGCCGCGCCTGCGCTGCG                                                          <  SRR4302543.4435599/94‑1 (MQ=60)
                                   TCGCGTTATTTCAACTCAAGATACCGACGTTACGCCGTTCGATCCCGGCGCATTTGTCTCACGCCAGAGCTATGTTGCCGCGCCTGCGCTGCGC                                                         >  SRR4302543.665034/1‑94 (MQ=60)
                                    TGCGTTATTTCAACTCAAGATACCGACGTTCCGCCGTTCGATCCCGGCGCATTTGTCTCACGCCAGAGCTATGTTGCCGCGCCTGCGCTGCGCA                                                        <  SRR4302543.4195972/94‑1 (MQ=60)
                                    CGCGTTATTTCAACTCAAGATACCGACGTTACGCCGTTCGATCCCGGCGCATTTGTCTCACGCCAGAGCTATGTTGCCGCGCCTGCGCTGCGCA                                                        <  SRR4302543.6787264/94‑1 (MQ=60)
                                    CGCGTTATTTCAACTCAAGATACCGACGTTACGCCGTTCGATCCCGGCGCATTTGTCTCACGCCAGAGCTATGTTGCCGCGCCTGCGCTGCGCA                                                        <  SRR4302543.6512280/94‑1 (MQ=60)
                                     GCGTTATTTCAACTCAAGATACCGACGTTACGCCGTTCGATCCCGGCGCATTTGTCTCACGCCAGAGCTATGTTGCCGCGCCTGCGCTGCGCAG                                                       >  SRR4302543.1687125/1‑94 (MQ=60)
                                     GCGTTATTTCAACTCAAGATACCGACGTTACGCCGTTCGATCCCGGCGCATTTGTCTCACGCCAGAGCTATGTTGCCGCGCCTGCGCTGCGCAG                                                       >  SRR4302543.5584662/1‑94 (MQ=60)
                                     GCGTTATTTCAACTCAAGATACCGACGTTACGCCGTTCGATCCCGGCGCATTTGTCTCACGCCAGAGCTATGTTGCCGCGCCTGCGCTGCGCAG                                                       >  SRR4302543.3895085/1‑94 (MQ=60)
                                     GCGTTATTTCAACTCAAGATACCGACGTTACGCCGTTCGATCCCGGCGCATTTGTCTCACGCCAGAGCTATGTTGCCGCGCCTGCGCTGCGCAG                                                       >  SRR4302543.834886/1‑94 (MQ=60)
                                     GCGTTATTTCAACTCAAGATACCGACGTTACGCCGTTCGATCCCGGCGCATTTGTCTCACGCCAGAGCTATGTTGCCGCGCCTGCGCTGCGCAG                                                       <  SRR4302543.2232110/94‑1 (MQ=60)
                                      CGTTATTTCAACTCAAGATACCGACGTTACGCCGTTCGATCCCGGCGCATTTGTCTCACGCCAGAGCTATGTTGCCGCGCCTGCGCTGCGCAGT                                                      >  SRR4302543.2769249/1‑94 (MQ=60)
                                      CGTTATTTCAACTCAAGATACCGACGTTACGCCGTTCGATCCCGGCGCATTTGTCTCACGCCAGAGCTATGTTGCCGCGCCTGCGCTGCGCAGT                                                      <  SRR4302543.551874/94‑1 (MQ=60)
                                      CGTTATTTCAACTCAAGATACCGACGTTACGCCGTTCGATCCCGGCGCATTTGTCTCACGCCAGAGCTATGTTGCCGCGCCTGCGCTGCGCAGT                                                      >  SRR4302543.1806161/1‑94 (MQ=60)
                                      CGTTATTTCAACTCAAGATACCGACGTTACGCCGTTCGATCCCGGCGCATTTGTCTCACGCCAGAGCTATGTTGCCGCGCCTGCGCTGCGCAGT                                                      >  SRR4302543.3603169/1‑94 (MQ=60)
                                       GTTATTTCAACTCAAGATACCGACGTTACGCCGTTCGATCCCGGCGCATTTGTCTCACGCCAGAGCTATGTTGCCGCGCCTGCGCTGCGCAGTG                                                     >  SRR4302543.2942373/1‑94 (MQ=60)
                                       GTTATTTCAACTCAAGATACCGACGTTACGCCGTTCGATCCCGGCGCATTTGTCTCACGCCAGAGCTATGTTGCCGCGCCTGCGCTGCGCAGTG                                                     >  SRR4302543.1508944/1‑94 (MQ=60)
                                        TTATTTCAACTCAAGATACCGACGTTACGCCGTTCGATCCCGGCGCATTTGTCTCACGCCAGAGCTATGTTGCCGCGCCTGCGCTGCGCAGTGC                                                    >  SRR4302543.913069/1‑94 (MQ=60)
                                        TTATTTCAACTCAAGATACCGACGTTACGCCGTTCGATCCCGGCGCATTTGTCTCACGCCAGAGCTATGTTGCCGCGCCTGCGCTGCGCAGTGC                                                    >  SRR4302543.1781284/1‑94 (MQ=60)
                                        TTATTTCAACTCAAGATACCGACGTTACGCCGTTCGATCCCGGCGCATTTGTCTCACGCCAGAGCTATGTTGCCGCGCCTGCGCTGCGCAGTGC                                                    >  SRR4302543.3030999/1‑94 (MQ=60)
                                        TTATTTCAACTCAAGATACCGACGTTACGCCGTTCGATCCCGGCGCATTTGTCTCACGCCAGAGCTATGTTGCCGCGCCTGCGCTGCGCAGTGC                                                    >  SRR4302543.4796890/1‑94 (MQ=60)
                                         TATTTCAACTCAAGATACCGACGTTACGCCGTTCGATCCCGGCGCATTTGTCTCACGCCAGAGCTATGTTGCCGCGCCTGCGCTGCGCAGTGCG                                                   >  SRR4302543.6718750/1‑94 (MQ=60)
                                         TATTTCAACTCAAGATACCGACGTTACGCCGTTCGATCCCGGCGCATTTGTCTCACGCCAGAGCTATGTTGCCGCGCCTGCGCTGCGCAGTGCG                                                   >  SRR4302543.7224824/1‑94 (MQ=60)
                                         TATTTCAACTCAAGATACCGACGTTACGCCGTTCGATCCCGGCGCATTTGTCTCACGCCAGAGCTATGTTGCCGCGCCTGCGCTGCGCAGTGCG                                                   <  SRR4302543.3119767/94‑1 (MQ=60)
                                          ATTTCAACTCAAGATACCGACGTTACGCCGTTCGATCCCGGCGCATTTGTCTCACGCCAGAGCTATGTTGCCGCGCCTGCGCTGCGCAGTGCGG                                                  >  SRR4302543.3185617/1‑94 (MQ=60)
                                          ATTTCAACTCAAGATACCGACGTTACGCCGTTCGATCCCGGCGCATTTGTCTCACGCCAGAGCTATGTTGCCGCGCCTGCGCTGCGCAGTGCGG                                                  >  SRR4302543.2627253/1‑94 (MQ=60)
                                            TTCAACTCAAGATACCGACGTTACGCCGTTCGATCCCGGCGCATTTGTCTCACGCCAGAGCTATGTTGCCGCGCCTGCGCTGCGCAGTGCGGCA                                                >  SRR4302543.5370545/1‑94 (MQ=60)
                                            TTCAACTCAAGATACCGACGTTACGCCGTTCGATCCCGGCGCATTTGTCTCACGCCAGAGCTATGTTGCCGCGCCTGCGCTGCGCAGTGCGGCA                                                >  SRR4302543.61275/1‑94 (MQ=60)
                                              CAACTCAAGATACCGACGTTACGCCGTTCGATCCCGGCGCATTTGTCTCACGCCAGAGCTATGTTGCCGCGCCTGCGCTGCGCAGTGCGGCACT                                              <  SRR4302543.6881846/94‑1 (MQ=60)
                                              CAACTCAAGATACCGACGTTACGCCGTTCGATCCCGGCGCATTTGTCTCACGCCAGAGCTATGTTGCCGCGCCTGCGCTGCGCAGTGCGGCACT                                              <  SRR4302543.6881845/94‑1 (MQ=60)
                                               AACTCAAGATACCGACGTTACGCCGTTCGATCCCGGCGCATTTGTCTCACGCCAGAGCTATGTTGCCGCGCCTGCGCTGCGCAGTGCGGCACTA                                             >  SRR4302543.5097299/1‑94 (MQ=60)
                                                ACTCAAGATACCGACGTTACGCCGTTCGATCCCGGCGCATTTGTCTCACGCCAGAGCTATGTTGCCGCGCCTGCGCTGCGCAGTGCGGCACTAT                                            <  SRR4302543.472161/94‑1 (MQ=60)
                                                 CTCAAGATACCGACGTTACGCCGTTCGATCCCGGCGCATTTGTCTCACGCCAGAGCTATGTTGCCGCGCCTGCGCTGCGCAGTGCGGCACTATT                                           >  SRR4302543.2671984/1‑94 (MQ=60)
                                                 CTCAAGATACCGACGTTACGCCGTTCGATCCCGGCGCATTTGTCTCACGCCAGAGCTATGTTGCCGCGCCTGCGCTGCGCAGTGCGGCACTATT                                           >  SRR4302543.5187605/1‑94 (MQ=60)
                                                 CTCAAGATACCGACGTTACGCCGTTCGATCCCGGCGCATTTGTCTCACGCCAGAGCTATGTTGCCGCGCCTGCGCTGCGCAGTGCGGCACTATT                                           <  SRR4302543.7968936/94‑1 (MQ=60)
                                                 CTCAAGATACCGACGTTACGCCGTTCGATCCCGGCGCATTTGTCTCACGCCAGAGCTATGTTGCCGCGCCTGCGCTGCGCAGTGCGGCACTATT                                           >  SRR4302543.5553561/1‑94 (MQ=60)
                                                 CTCAAGATACCGACGTTACGCCGTTCGATCCCGGCGCATTTGTCTCACGCCAGAGCTATGCTGCCGCGCCTGCGCTGCGCAGTGCGGCACTATT                                           >  SRR4302543.5553558/1‑94 (MQ=60)
                                                   CAAGATACCGACGTTACGCCGTTCGATCCCGGCGCATTTGTCTCACGCCAGAGCTATGTTGCCGCGCCTGCGCTGCGCAGTGCGGCCCTATTAT                                         >  SRR4302543.2990684/1‑94 (MQ=60)
                                                   CAAGATACCGACGTTACGCCGTTCGATCCCGGCGCATTTGTCTCACGCCAGAGCTATGTTGCCGCGCCTGCGCTGCGCAGTGCGGCACTATTAT                                         >  SRR4302543.7756336/1‑94 (MQ=60)
                                                   CAAGATACCGACGTTACGCCGTTCGATCCCGGCGCATTTGTCTCACGCCAGAGCTATGTTGCCGCGCCTGCGCTGCGCAGTGCGGCACTATTAT                                         >  SRR4302543.7685215/1‑94 (MQ=60)
                                                   CAAGATACCGACGTTACGCCGTTCGATCCCGGCGCATTTGTCTCACGCCAGAGCTATGTTGCCGCGCCTGCGCTGCGCAGTGCGGCACTATTAT                                         <  SRR4302543.766310/94‑1 (MQ=60)
                                                   CAAGATACCGACGTTACGCCGTTCGATCCCGGCGCATTTGTCTCACGCCAGAGCTATGTTGCCGCGCCTGCGCTGCGCAGTGCGGCACTATTAT                                         <  SRR4302543.112297/94‑1 (MQ=60)
                                                   CAAGATACCGACGTTACGCCGTTCGATCCCGGCGCATTTGTCTCACGCCAGAGCTATGTTGCCGCGCCTGCGCTGCGCAGTGCGGCACTATTAT                                         >  SRR4302543.5268527/1‑94 (MQ=60)
                                                     AGATACCGACGTTACGCCGTTCGATCCCGGCGCATTTGTCTCACGCCAGAGCTATGTTGCCGCGCCTGCGCTGCGCAGTGCGGCACTATTATTA                                       >  SRR4302543.911941/1‑94 (MQ=60)
                                                     AGATACCGACGTTACGCCGTTCGATCCCGGCGCATTTGTCTCACGCCAGAGCTATGTTGCCGCGCCTGCGCTGCGCAGTGCGGCACTATTATTA                                       >  SRR4302543.5369303/1‑94 (MQ=60)
                                                     AGATACCGACGTTACGCCGTTCGATCCCGGCGCATTTGTCTCACGCCAGAGCTATGTTGCCGCGCCTGCGCTGCGCAGTGCGGCACTATTATTA                                       <  SRR4302543.2560562/94‑1 (MQ=60)
                                                      GATACCGACGTTACGCCGTTCGATCCCGGCGCATTTGTCTCACGCCAGAGCTATGTTGCCGCGCCTGCGCTGCGCAGTGCGGCACTATTATTAA                                      <  SRR4302543.5430749/94‑1 (MQ=60)
                                                      GATACCGACGTTACGCCGTTCGATCCCGGCGCATTTGTCTCACGCCAGAGCTATGTTGCCGCGCCTGCGCTGCGCAGTGCGGCACTATTATTAA                                      >  SRR4302543.507755/1‑94 (MQ=60)
                                                        TACCGACGTTACGCCGTTCGATCCCGGCGCATTTGTCTCACGCCAGAGCTATGTTGCCGCGCCTGCGCTGCGCAGTGCGGCACTATTATTAAAA                                    <  SRR4302543.4690129/94‑1 (MQ=60)
                                                        TACCGACGTTACGCCGTTCGATCCCGGCGCATTTGTCTCACGCCAGAGCTATGTTGCCGCGCCTGCGCTGCGCAGTGCGGCACTATTATTAAAA                                    <  SRR4302543.7176702/94‑1 (MQ=60)
                                                         TCCGACGTTCCGCCGTTCGATCCCGGCGCATTTGTCTCACGCCAGAGCTATGTTGCCGCGCCTGCGCTGCGCAGTGCGGCACTATTATTAAAAG                                   <  SRR4302543.6054072/94‑1 (MQ=60)
                                                         ACCGACGTTACGCCGTTCGATCCCGGCGCATTTGTCTCACGCCAGAGCTATGTTGCCGCGCCTGCGCTGCGCAGTGCGGCACTATTATTAAAAG                                   <  SRR4302543.6501850/94‑1 (MQ=60)
                                                           CGACGTTACGCCGTTCGATCCCGGCGCATTTGTCTCACGCCAGAGCTATGTTGCCGCGCCTGCGCTGCGCAGTGCGGCACTATTATTAAAAGAG                                 <  SRR4302543.595167/94‑1 (MQ=60)
                                                           CGACGTTACGCCGTTCGATCCCGGCGCATTTGTCTCACGCCAGAGCTATGTTGCCGCGCCTGCGCTGCGCAGTGCGGCACTATTATTAAAAGAG                                 >  SRR4302543.404741/1‑94 (MQ=60)
                                                           CGACGTTACGCCGTTCGATCCCGGCGCATTTGTCTCACGCCAGAGCTATGTTGCCGCGCCTGCGCTGCGCAGTGCGGCACTATTATTAAAAGAG                                 <  SRR4302543.3820493/94‑1 (MQ=60)
                                                           CGACGTTACGCCGTTCGATCCCGGCGCATTTGTCTCACGCCAGAGCTATGTTGCCGCGCCTGCGCTGCGCAGTGCGGCACTATTATTAAAAGAG                                 >  SRR4302543.5203593/1‑94 (MQ=60)
                                                           CGACGTTACGCCGTTCGATCCCGGCGCATTTGTCTCACGCCAGAGCTATGTTGCCGCGCCTGCGCTGCGCAGTGCGGCACTATTATTAAAAGAG                                 <  SRR4302543.5166874/94‑1 (MQ=60)
                                                            GACGTTACGCCGTTCGATCCCGGCGCATTTGTCTCACGCCAGAGCTATGTTGCCGCGCCTGCGCTGCGCAGTGCGGCACTATTATTAAAAGAGA                                >  SRR4302543.6233043/1‑94 (MQ=60)
                                                              CGTTACGCCGTTCGATCCCGGCGCATTTGTCTCACGCCAGAGCTATGTTGCCGCGCCTGCGCTGCGCAGTGCGGCACTATTATTAAAAGAGAAA                              <  SRR4302543.4810640/94‑1 (MQ=60)
                                                              CGTTACGCCGTTCGATCCCGGCGCATTTGTCTCACGCCAGAGCTATGTTGCCGCGCCTGCGCTGCGCAGTGCGGCACTATTATTAAAAGAGAAA                              >  SRR4302543.5037598/1‑94 (MQ=60)
                                                               GTTACGCCGTTCGATCCCGGCGCATTTGTCTCACGCCAGAGCTATGTTGCCGCGCCTGCGCTGCGCAGTGCGGCACTATTATTAAAAGAGAAAA                             <  SRR4302543.981517/94‑1 (MQ=60)
                                                               GTTACGCCGTTCGATCCCGGCGCATTTGTCTCACGCCAGAGCTATGTTGCCGCGCCTGCGCTGCGCAGTGCGGCACTATTATTAAAAGAGAAAA                             <  SRR4302543.5871945/94‑1 (MQ=60)
                                                                TTACGCCGTTCGATCCCGGCGCATTTGTCTCACGCCAGAGCTATGTTGCCGCGCCTGCGCTGCGCAGTGCGGCACTATTATTAAAAGAGAAAAT                            <  SRR4302543.952759/94‑1 (MQ=60)
                                                                TTACGCCGTTCGATCCCGGCGCATTTGTCTCACGCCAGAGCTATGTTGCCGCGCCTGCGCTGCGCAGTGCGGCACTATTATTAAAAGAGAAAAT                            <  SRR4302543.12494/94‑1 (MQ=60)
                                                                TTACGCCGTTCGATCCCGGCGCATTTGTCTCACGCCAGAGCTATGTTGCCGCGCCTGCGCTGCGCAGTGCGGCACTATTATTAAAAGAGAAAAT                            <  SRR4302543.3859931/94‑1 (MQ=60)
                                                                GTACGCCGTTCGATCCCGGCGCATTTGTCTCACGCCAGAGCTATGTTGCCGCGCCTGCGCTGCGCAGTGCGGCACTATTATTAAAAGAGAAAAT                            <  SRR4302543.6345996/94‑1 (MQ=60)
                                                                  ACGCCGTTCGATCCCGGCGCATTTGTCTCACGCCAGAGCTATGTTGCCGCGCCTGCGCTGCGCAGTGCGGCACTATTATTAAAAGAGAAAATCA                          >  SRR4302543.955802/1‑94 (MQ=60)
                                                                  ACGCCGTTCGATCCCGGCGCATTTGTCTCACGCCAGAGCTATGTTGCCGCGCCTGCGCTGCGCAGTGCGGCACTATTATTAAAAGAGAAAATCA                          <  SRR4302543.5625537/94‑1 (MQ=60)
                                                                   CGCCGTTCGATCCCGGCGCATTTGTCTCACGCCAGAGCTATGTTGCCGCGCCTGCGCTGCGCAGTGCGGCACTATTATTAAAAGAGAAAATCAT                         <  SRR4302543.7588826/94‑1 (MQ=60)
                                                                   CGCCGTTCGATCCCGGCGCATTTGTCGCACGCCAGAGCTATGTTGCCGCGCCTGCGCTGCGCAGTGCGGCACTATTATTAAAAGAGAAAATCAT                         <  SRR4302543.3939184/94‑1 (MQ=60)
                                                                    GCCGTTCGATCCCGGCGCATTTGTCTCACGCCAGAGCTATGTTGCCGCGCCTGCGCTGCGCAGTGCGGCACTATTATTAAAAGAGAAAATCATC                        <  SRR4302543.2031296/94‑1 (MQ=60)
                                                                    GCCGTTCGATCCCGGCGCATTTGTCTCACGCCAGAGCTATGTTGCCGCGCCTGCGCTGCGCAGTGCGGCACTATTATTAAAAGAGAAAATCATC                        <  SRR4302543.5384092/94‑1 (MQ=60)
                                                                    GCCGTTCGATCCCGGCGCATTTGTCTCACGCCAGAGCTATGTTGCCGCGCCTGCGCTGCGCAGTGCGGCACTATTATTAAAAGAGAAAATCATC                        <  SRR4302543.2546795/94‑1 (MQ=60)
                                                                     CCGTTCGATCCCGGCGCATTTGTCTCACGCCAGAGCTATGTTGCCGCGCCTGCGCTGCGCAGTGCGGCACTATTATTAAAAGAGAAAATCATCG                       <  SRR4302543.5672943/94‑1 (MQ=60)
                                                                     CCGTTCGATCCCGGCGCATTTGTCTCACGCCAGAGCTATGTTGCCGCGCCTGCGCTGCGCAGTGCGGCACTATTATTAAAAGAGAAAATCATCG                       <  SRR4302543.703800/94‑1 (MQ=60)
                                                                      CGTTCGATCCCGGCGCATTTGTCTCACGCCAGAGCTATGTTGCCGCGCCTGCGCTGCGCAGTGCGGCACTATTATTAAAAGAGAAAATCATCGC                      <  SRR4302543.3287120/94‑1 (MQ=60)
                                                                      CGTTCGATCCCGGCGCATTTGTCTCACGCCAGAGCTATGTTGCCGCGCCTGCGCTGCGCAGTGCGGCACTATTATTAAAAGAGAAAATCATCGC                      >  SRR4302543.4063521/1‑94 (MQ=60)
                                                                      CGTTCGATCCCGGCGCATTTGTCTCACGCCAGAGCTATGTTGCCGCGCCTGCGCTGCGCAGTGCGGCACTATTATTAAAAGAGAAAATCATCGC                      <  SRR4302543.6164833/94‑1 (MQ=60)
                                                                       GTTCGATCCCGGCGCATTTGTCTCACGCCAGAGCTATGTTGCCGCGCCTGCGCTGCGCAGTGCGGCACTATTATTAAAAGAGAAAATCATCGCT                     >  SRR4302543.5595728/1‑94 (MQ=60)
                                                                       GTTCGATCCCGGCGCATTTGTCTCACGCCAGAGCTATGTTGCCGCGCCTGCGCTGCGCAGTGCGGCACTATTATTAAAAGAGAAAATCATCGCT                     <  SRR4302543.5698293/94‑1 (MQ=60)
                                                                       GTTCGATCCCGGCGCATTTGTCTCACGCCAGAGCTATGTTGCCGCGCCTGCGCTGCGCAGTGCGGCACTATTATTAAAAGAGAAAATCATCGCT                     >  SRR4302543.5893027/1‑94 (MQ=60)
                                                                       GTTCGATCCCGGCGCATTTGTCTCACGCCAGAGCTATGTTGCCGCGCCTGCGCTGCGCAGTGCGGCACTATTATTAAAAGAGAAAATCATCGCT                     >  SRR4302543.2629737/1‑94 (MQ=60)
                                                                       GTTCGATCCCGGCGCATTTGTCTCACGCCAGAGCTATGTTGCCGCGCCTGCGCTGCGCAGTGCGGCACTATTATTAAAAGAGAAAATCATCGCT                     >  SRR4302543.2699543/1‑94 (MQ=60)
                                                                       GTTCGAGCCCGGCGCATTTGCCTCACGCCAGAGCTATGTTGCCGCGCCTGCGCTGCGCAGTGCGGCACTATTATTAAAAGAGAAAATCATCGCT                     <  SRR4302543.1586735/94‑1 (MQ=60)
                                                                          CGATCCCGGCGCATTTGTCTCACGCCAGAGCTATGTTGCCGCGCCTGCGCTGCGCAGTGCGGCACTATTATTAAAAGAGAAAATCATCGCTCAC                  <  SRR4302543.682545/94‑1 (MQ=60)
                                                                          CGATCCCGGCGCATTTGTCTCACGCCAGAGCTATGTTGCCGCGCCTGCGCTGCGCAGTGCGGCACTATTATTAAAAGAGAAAATCATCGCTCAC                  <  SRR4302543.6151454/94‑1 (MQ=60)
                                                                          CGATCCCGGCGCATTTGTCTCACGCCAGAGCTATGTTGCCGCGCCTGCGCTGCGCAGTGCGGCACTATTATTAAAAGAGAAAATCATCGCTCAC                  <  SRR4302543.884908/94‑1 (MQ=60)
                                                                           GATCCCGGCGCATTTGTCTCACGCCAGAGCTATGTTGCCGCGCCTGCGCTGCGCAGTGCGGCACTATTATTAAAAGAGAAAATCATCGCTCACG                 >  SRR4302543.1364512/1‑94 (MQ=60)
                                                                            ATCCCGGCGCATTTGTCTCACGCCAGAGCTATGTTGCCGCGCCTGCGCTGCGCAGTGCGGCACTATTATTAAAAGAGAAAATCATCGCTCACGC                <  SRR4302543.6424165/94‑1 (MQ=60)
                                                                             TCCCGGCGCATTTGTCTCACGCCAGAGCTATGTTGCCGCGCCTGCGCTGCGCAGTGCGGCACTATTATTAAAAGAGAAAATCATCGCTCACGCC               <  SRR4302543.7757557/94‑1 (MQ=60)
                                                                             TCCCGGCGCATTTGTCTCACGCCAGAGCTATGTTGCCGCGCCTGCGCTGCGCAGTGCGGCACTATTATTAAAAGAGAAAATCATCGCNCACGCC               >  SRR4302543.4330537/1‑94 (MQ=60)
                                                                              CCCGGCGCATTTGTCTCACGCCAGAGCTATGTTGCCGCGCCTGCGCTGCGCAGTGCGGCACTATTATTAAAAGAGAAAATCATCGCTCACGCCG              >  SRR4302543.552511/1‑94 (MQ=60)
                                                                                 GGCGCATTTGTCTCACGCCAGAGCTATGTTGCCGCGCCTGCGCTGCGCAGTGCGGCACTATTATTAAAAGAGAAAATCATCGCTCACGCCGCAG           >  SRR4302543.3253511/1‑94 (MQ=60)
                                                                                 GGCGCATTTGTCTCACGCCAGAGCTATGTTGCCGCGCCTGCGCTGCGCAGTGCGGCACTATTATTAAAAGAGAAAATCATCGCTCACGCCGCAG           >  SRR4302543.3253513/1‑94 (MQ=60)
                                                                                    GCATTTGTCTCACGCCAGAGCTATGTTGCCGCGCCTGCGCTGCGCAGTGCGGCACTATTATTAAAAGAGAAAATCATCGCTCACGCCGCAGTCA        >  SRR4302543.5361953/1‑94 (MQ=60)
                                                                                     CATTTGTCTCACGCCAGAGCTATGTTGCCGCGCCTGCGCTGCGCAGTGCGGCACTATTATTAAAAGAGAAAATCATCGCTCACGCCGCAGTCAT       <  SRR4302543.1137204/94‑1 (MQ=60)
                                                                                     CATTTGTCTCACGCCAGAGCTATGTTGCCGCGCCTGCGCTGCGCAGTGCGGCACTATTATTAAAAGAGAAAATCATCGCTCACGCCGCAGTCAT       <  SRR4302543.3310905/94‑1 (MQ=60)
                                                                                      ATTTGTCTCACGCCAGAGCTATGTTGCCGCGCCTGCGCTGCGCAGTGCGGCACTATTATTAAAAGAGAAAATCATCGCTCACGCCGCAGTCATG      >  SRR4302543.228157/1‑94 (MQ=60)
                                                                                      ATTTGTCTCACGCCAGAGCTATGTTGCCGCGCCTGCGCTGCGCAGTGCGGCACTATTATTAAAAGAGAAAATCATCGCTCACGCCGCAGTCATG      <  SRR4302543.885792/94‑1 (MQ=60)
                                                                                      ATTTGTCTCACGCCAGAGCTATGTTGCCGCGCCTGCGCTGCGCAGTGCGGCACTATTATTAAAAGAGAAAATCATCGCTCACGCCGCAGTCATG      >  SRR4302543.6825237/1‑94 (MQ=60)
                                                                                      ATTTGTCTCACGCCAGAGCTATGTTGCCGCGCCTGCGCTGCGCAGTGCGGCACTATTATTAAAAGAGAAAATCATCGCTCACGCCGCAGTCATG      >  SRR4302543.5947847/1‑94 (MQ=60)
                                                                                       TTTGTCTCACGCCAGAGCTATGTTGCCGCGCCTGCGCTGCGCAGTGCGGCACTATTATTAAAAGAGAAAATCATCGCTCACGCCGCAGTCATGC     >  SRR4302543.7191193/1‑94 (MQ=60)
                                                                                        TTGTCTCACGCCAGAGCTATGTTGCCGCGCCTGCGCTGCGCAGTGCGGCACTATTATTAAAAGAGAAAATCATCGCTCACGCCGCAGTCATGCT    <  SRR4302543.4627924/94‑1 (MQ=60)
                                                                                         TGTCTCACGCCAGAGCTATGTTGCCGCGCCTGCGCTGCGCAGTGCGGCACTATTATTAAAAGAGAAAATCATCGCTCACGCCGCAGTCATGCTA   <  SRR4302543.7858685/94‑1 (MQ=60)
                                                                                          GTCTCACGCCAGAGCTATGTTGCCGCGCCTGCGCTGCGCAGTGCGGCACTATTATTAAAAGAGAAAATCATCGCTCACGCCGCAGTCATGCTAC  <  SRR4302543.2951779/94‑1 (MQ=60)
                                                                                           |                                                                                            
GTGGCAGAAACCGTGGGGGTTCCGGTCAGCGACGTTCGCGTTATTTCAACTCAAGATACCGACGTTACGCCGTTCGATCCCGGCGCATTTGCCTCACGCCAGAGCTATGTTGCCGCGCCTGCGCTGCGCAGTGCGGCACTATTATTAAAAGAGAAAATCATCGCTCACGCCGCAGTCATGCTAC  >  NC_000913/2999822‑3000005

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑