Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I1 R1	1696	279.3	15874187	97.8%	15524954	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,049,165	T→C	G391G (GGA→GGG)	visC ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,049,165	0	T	C	99.6%	768.6 / ‑5.6	241	G391G (GGA→GGG)	visC	hypothetical protein
Reads supporting (aligned to +/- strand): ref base T (1/0); new base C (121/119); total (122/119)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01

Only 100 of 247 total aligned reads displayed.

GCGAAAAAACCGGGAGATGAAAGTGTGATGGGTATCAAATAAACAACAGAGGAGAAATTTTTAACGCAGCCATTCAGGCAAATCGTTTAATCCCATTGCCTGGCGGATAAGTTGCGGCTTAACGCCAGGAAGCGTGTCGGCCAGTTTCAAACCAATATCACGCAGCAGTTTTTTCGCCGG  >  NC_000913/3049075‑3049254

gCGAAAAAACCGGGAGATGAAAGTGTGATGGGTATCAAATAAACAACAGAGGAGAAATTTTTAACGCAGCCATTCAGGCAAATCGTTTAAccc                                                                                         >  2:3147773/1‑93 (MQ=255)

  gAAAAAACCGGGAGATGAAAGTGTGATGGGTATCAAATAAACAACAGAGGAGAAATTTTTAACGCAGCCATTCAGGCAAATCGTTTAACCCCa                                                                                       >  2:2621743/1‑93 (MQ=255)

   aaaaaaCCGGGAGATGAAAGTGTGATGGGTATCAAATAAACAACAGAGGAGAAATTTTTAACGCAGCCATTCAGGCAAATCGTTTAACCCCAt                                                                                      <  2:5875621/93‑1 (MQ=255)

    aaaaaCCGGGAGATGAAAGTGTGATGGGTATCAAATAAACAACAGAGGAGAAATTTTTAACGCAGCCATTCAGGCAAATCGTTTAACCCCAtt                                                                                     <  2:3780776/93‑1 (MQ=255)

    aaaaaCCGGGAGATGAAAGTGTGATGGGTATCAAATAAACAACAGAGGAGAAATTTTTAACGCAGCCATTCAGGCAAATCGTTTAACCCCAtt                                                                                     <  1:868355/93‑1 (MQ=255)

     aaaaCCGGGAGATGAAAGTGTGATGGGTATCAAATAAACAACAGAGGAGAAATTTTTAACGCAGCCATTCAGGCAAATCGTTTAACCCCATTg                                                                                    <  2:5575076/93‑1 (MQ=255)

      aaaCCGGGAGATGAAAGTGTGATGGGTATCAAATAAACAACAGAGGAGAAATTTTTAACGCAGCCATTCAGGCAAATCGTTTAACCCCATTGc                                                                                   >  1:2652204/1‑93 (MQ=255)

        aCCGGGAGATGAAAGTGTGATGGGTATCAAATAAACAACAGAGGAGAAATTTTTAACGCAGCCATTCAGGCAAATCGTTTAACCCCATTGCCt                                                                                 <  1:6068254/93‑1 (MQ=255)

        aCCGGGAGATGAAAGTGTGATGGGTATCAAATAAACAACAGAGGAGAAATTTTTAACGCAGCCATTCAGGCAAATCGTTTAACCCCATTGCCt                                                                                 >  1:3062291/1‑93 (MQ=255)

        aCCGGGAGATGAAAGTGTGATGGGTATCAAATAAACAACAGAGGAGAAATTTTTAACGCAGCCATTCAGGCAAATCGTTTAACCCCATTGCCt                                                                                 <  2:2028729/93‑1 (MQ=255)

         ccGGGAGATGAAAGTGTGATGGGTATCAAATAAACAACAGAGGAGAAATTTTTAACGCAGCCATTCAGGCAAATCGTTTAACCCCATTGCCTg                                                                                <  2:5624975/93‑1 (MQ=255)

           gggAGATGAAAGTGTGATGGGTATCAAATAAACAACAGAGGAGAAATTTTTAACGCAGCCATTCAGGCAAATCGTTTAACCCCATTGCCTGGc                                                                              <  2:5152678/93‑1 (MQ=255)

            ggAGATGAAAGTGTGATGGGTATCAAATAAACAACAGAGGAGAAATTTTTAACGCAGCCATTCAGGCAAATCGTTTAACCCCATTGCCTGGCg                                                                             <  1:7844627/93‑1 (MQ=255)

               gaTGAAAGTGTGATGGGTATCAAATAAACAACAGAGGAGAAATTTTTAACGCAGCCATTCAGGCAAATCGTTTAACCCCATTGCCTGGCGGAt                                                                          >  1:5147100/1‑93 (MQ=255)

                aTGAAAGTGTGATGGGTATCAAATAAACAACAGAGGAGAAATTTTTAACGCAGCCATTCAGGCAAATCGTTTAACCCCATTGCCTGGCGGATa                                                                         <  1:829865/93‑1 (MQ=255)

                 tGAAAGTGTGATGGGTATCAAATAAACAACAGAGGAGAAATTTTTAACGCAGCCATTCAGGCAAATCGTTTAACCCCATTGCCTGGCGGATaa                                                                        >  2:1867028/1‑93 (MQ=255)

                 tGAAAGTGTGATGGGTATCAAATAAACAACAGAGGAGAAATTTTTAACGCAGCCATTCAGGCAAATCGTTTAACCCCATTGCCTGGCGGATaa                                                                        >  1:4667772/1‑93 (MQ=255)

                  gAAAGTGTGATGGGTATCAAATAAACAACAGAGGAGAAATTTTTAACGCAGCCATTCAGGCAAATCGTTTAACCCCATTGCCTGGCGGATAAg                                                                       >  2:452501/1‑93 (MQ=255)

                    aaGTGTGATGGGTATCAAATAAACAACAGAGGAGAAATTTTTAACGCAGCCATTCAGGCAAATCGTTTAACCCCATTGCCTGGCGGATAAGtt                                                                     <  2:2276178/93‑1 (MQ=255)

                    aaGTGTGATGGGTATCAAATAAACAACAGAGGAGAAATTTTTAACGCAGCCATTCAGGCAAATCGTTTAACCCCATTGCCTGGCGGATAAGtt                                                                     >  2:5942277/1‑93 (MQ=255)

                      gtgtGATGGGTATCAAATAAACAACAGAGGAGAAATTTTTAACGCAGCCATTCAGGCAAATCGTTTAACCCCATTGCCTGGCGGATAAGTTGc                                                                   <  2:287108/93‑1 (MQ=255)

                      gtgtGATGGGTATCAAATAAACAACAGAGGAGAAATTTTTAACGCAGCCATTCAGGCAAATCGTTTAACCCCATTGCCTGGCGGATAAGTTGc                                                                   <  1:7500828/93‑1 (MQ=255)

                        gtgATGGGTATCAAATAAACAACAGAGGAGAAATTTTTAACGCAGCCATTCAGGCAAATCGTTTAACCCCATTGCCTGGCGGATAAGTTGCgg                                                                 >  2:2355704/1‑93 (MQ=255)

                        gtgATGGGTATCAAATAAACAACAGAGGAGAAATTTTTAACGCAGCCATTCAGGCAAATCGTTTAACCCCATTGCCTGGCGGATAAGTTGCgg                                                                 >  1:5937036/1‑93 (MQ=255)

                         tgATGGGTATCAAATAAACAACAGAGGAGAAATTTTTAACGCAGCCATTCAGGCAAATCGTTTAACCCCATTGCCTGGCGGATAAGTTGCGGc                                                                <  2:7201978/93‑1 (MQ=255)

                          gATGGGTATCAAATAAACAACAGAGGAGAAATTTTTAACGCAGCCATTCAGGCAAATCGTTTAACCCCATTGCCTGGCGGATAAGTTGCGGCt                                                               >  1:4984335/1‑93 (MQ=255)

                            tGGGTATCAAATAAACAACAGAGGAGAAATTTTTAACGCAGCCATTCAGGCAAATCGTTTAACCCCATTGCCTGGCGGATAAGTTGCGGCTTa                                                             >  2:3588120/1‑93 (MQ=255)

                            tGGGTATCAAATAAACAACAGAGGAGAAATTTTTAACGCAGCCATTCAGGCAAATCGTTTAACCCCATTGCCTGGCGGATAAGTTGCGGCTTa                                                             >  2:4710917/1‑93 (MQ=255)

                              ggTATCAAATAAGCAACAGAGGAGAAATTTTTAACGCAGCCATTCAGGCAAATCGTTTAACCCCATTGCCTGGCGGATAAGTTGCGGCTTAAc                                                           <  1:4959345/93‑1 (MQ=255)

                              ggTATCAAATAAACAACAGAGGAGAAATTTTTAACGCAGCCATTCAGGCAAATCGTTTAACCCCATTGCCTGGCGGATAAGTTGCGGCTTAAc                                                           >  2:7923837/1‑93 (MQ=255)

                               gTATCAAATAAACAACAGAGGAGAAATTTTTAACGCAGCCATTCAGGCAAATCGTTTAACCCCATTGCCTGGCGGATAAGTTGCGGCTTAACg                                                          >  1:587359/1‑93 (MQ=255)

                               gTATCAAATAAACAACAGAGGAGAAATTTTTAACGCAGCCATTCAGGCAAATCGTTTAACCCCATTGCCTGGCGGATAAGTTGCGGCTTAACg                                                          <  1:183753/93‑1 (MQ=255)

                                 aTCAAATAAACAACAGAGGAGAAATTTTTAACGCAGCCATTCAGGCAAATCGTTTAACCCCATTGCCTGGCGGATAAGTTGCGGCTTAACGcc                                                        >  2:6202302/1‑93 (MQ=255)

                                    aaaTAAACAACAGAGGAGAAATTTTTAACGCAGCCATTCAGGCAAATCGTTTAACCCCATTGCCTGGCGGATAAGTTGCGGCTTAACGCCAgg                                                     <  2:3504023/93‑1 (MQ=255)

                                     aaTAAACAACAGAGGAGAAATTTTTAACGCAGCCATTCAGGCAAATCGTTTAACCCCATTGCCTGGCGGATAAGTTGCGGCTTAACGCCAGGa                                                    <  2:1602703/93‑1 (MQ=255)

                                     aaTAAACAACAGAGGAGAAATTTTTAACGCAGCCATTCAGGCAAATCGTTTAACCCCATTGCCTGGCGGATAAGTTGCGGCTTAACGCCAGGa                                                    >  1:4064542/1‑93 (MQ=255)

                                     aaTAAACAACAGAGGAGAAATTTTTAACGCAGCCATTCAGGCAAATCGTTTAACCCCATTGCCTGGCGGATAAGTTGCGGCTTAACGCCAGGa                                                    <  1:2211989/93‑1 (MQ=255)

                                      aTAAACAACAGAGGAGAAATTTTTAACGCAGCCATTCAGGCAAATCGTTTAACCCCATTGCCTGGCGGATAAGTTGCGGCTTAACGCCAGGaa                                                   <  2:400440/93‑1 (MQ=255)

                                      aTAAACAACAGAGGAGAAATTTTTAACGCAGCCATTCAGGCAAATCGTTTAACCCCATTGCCTGGCGGATAAGTTGCGGCTTAACGCCAGGaa                                                   <  1:3527890/93‑1 (MQ=255)

                                        aaaCAACAGAGGAGAAATTTTTAACGCAGCCATTCAGGCAAATCGTTTAACCCCATTGCCTGGCGGATAAGTTGCGGCTTAACGCCAGGAAGc                                                 <  2:1153348/93‑1 (MQ=255)

                                         aacaacAGAGGAGAAATTTTTAACGCAGCCATTCAGGCAAATCGTTTAACCCCATTGCCTGGCGGATAAGTTGCGGCTTAACGCCAGGAAGCg                                                >  1:276968/1‑93 (MQ=255)

                                          acaacaGAGGAGAAATTTTTAACGCAGCCATTCAGGCAAATCGTTTAACCCCATTGCCTGGCGGATAAGTTGCGGCTTAACGCCAGGAAGCgt                                               <  1:2324652/93‑1 (MQ=255)

                                           caacaGAGGAGAAATTTTTAACGCAGCCATTCAGGCAAATCGTTTAACCCCATTGCCTGGCGGATAAGTTGCGGCTTAACGCCAGGAAGCgtg                                              <  1:6957715/93‑1 (MQ=255)

                                            aacaGAGGAGAAATTTTTAACGCAGCCATTCAGGCAAATCGTTTAACCCCATTGCCTGGCGGATAAGTTGCGGCTTAACGCCAGGAAGCgtgt                                             <  2:6782795/93‑1 (MQ=255)

                                            aacaGAGGAGAAATTTTTAACGCAGCCATTCAGGCAAATCGTTTAACCCCATTGCCTGGCGGATAAGTTGCGGCTTAACGCCAGGAAGCgtgt                                             <  1:6719928/93‑1 (MQ=255)

                                             acaGAGGAGAAATTTTTAACGCAGCCATTCAGGCAAATCGTTTAACCCCATTGCCTGGCGGATAAGTTGCGGCTTAACGCCAGGAAGCGTGTc                                            <  2:2611439/93‑1 (MQ=255)

                                                gaggagAAATTTTTAACGCAGCCATTCAGGCAAATCGTTTAACCCCATTGCCTGGCGGATAAGTTGCGGCTTAACGCCAGGAAGCGTGTCGGc                                         >  2:4975967/1‑93 (MQ=255)

                                                gaggagAAATTTTTAACGCAGCCATTCAGGCAAATCGTTTAACCCCATTGCCTGGCGGATAAGTTGCGGCTTAACGCCAGGAAGCGTGTCGGc                                         >  2:6658044/1‑93 (MQ=255)

                                                 aggagAAATTTTTAACGCAGCCATTCAGGCAAATCGTTTAACCCCATTGCCTGGCGGATAAGTTGCGGCTTAACGCCAGGAAGCGTGTCGGcc                                        >  2:7783288/1‑93 (MQ=255)

                                                  ggagAAATTTTTAACGCAGCCATTCAGGCAAATCGTTTAACCCCATTGCCTGGCGGATAAGTTGCGGCTTAACGCCAGGAAGCGTGTCGGCCa                                       <  1:1938/93‑1 (MQ=255)

                                                   gagaAATTTTTAACGCAGCCATTCAGGCAAATCGTTTAACCCCATTGCCTGGCGGATAAGTTGCGGCTTAACGCCAGGAAGCGTGTCGGCCAg                                      >  2:6312367/1‑93 (MQ=255)

                                                     gaAATTTTTAACGCAGCCATTCAGGCAAATCGTTTAACCCCATTGCCTGGCGGATAAGTTGCGGCTTAACGCCAGGAAGCGTGTCGGCCAGtt                                    >  1:3813292/1‑93 (MQ=255)

                                                      aaaTTTTTAACGCAGCCATTCAGGCAAATCGTTTAACCCCATTGCCTGGCGGATAAGTTGCGGCTTAACGCCAGGAAGCGTGTCGGCCAGttt                                   >  2:323322/1‑93 (MQ=255)

                                                         tttttAACGCAGCCATTCAGGCAAATCGTTTAACCCCATTGCCTGGCGGATAAGTTGCGGCTTAACGCCAGGAAGCGTGTCGGCCAGTTTCaa                                >  1:2343659/1‑93 (MQ=255)

                                                          ttttAACGCAGCCATTCAGGCAAATCGTTTAACCCCATTGCCTGGCGGATAAGTTGCGGCTTAACGCCAGGAAGCGTGTCGGCCAGTTTCaaa                               >  2:4809106/1‑93 (MQ=255)

                                                           tttAACGCAGCCATTCAGGCAAATCGTTTAACCCCATTGCCTGGCGGATAAGTTGCGGCTTAACGCCAGGAAGCGTGTCGGCCAGTTTCAAAc                              >  1:1204085/1‑93 (MQ=255)

                                                           tttAACGCAGCCATTCAGGCAAATCGTTTAACCCCATTGCCTGGCGGATAAGTTGCGGCTTAACGCCAGGAAGCGTGTCGGCCAGTTTCAAAc                              <  2:706072/93‑1 (MQ=255)

                                                           tttAACGCAGCCATTCAGGCAAATCGTTTAACCCCATTGCCTGGCGGATAAG‑TGCGGCTTAACGCCAGGAAGCGGGTCGGCCAGTTTCAAAcc                             >  1:1664253/1‑93 (MQ=255)

                                                            ttAACGCAGCCATTCAGGCAAATCGTTTAACCCCATTGCCTGGCGGATAAGTTGCGGCTTAACGCCAGGAAGCGTGTCGGCCAGTTTCAAAcc                             <  1:4863652/93‑1 (MQ=255)

                                                            ttAACGCAGCCATTCAGGCAAATCGTTTAACCCCATTGCCTGGCGGATAAGTTGCGGCTTAACGCCAGGAAGCGTGTCGGCCAGTTTCAAAcc                             >  2:3197083/1‑93 (MQ=255)

                                                              aaCGCAGCCATTCAGGCAAATCGTTTAACCCCATTGCCTGGCGGATAAGTTGCGGCTTAACGCCAGGAAGCGTGTCGGCCAGTTTCAAACCaa                           <  2:5796713/93‑1 (MQ=255)

                                                              aaCGCAGCCATTCAGGCAAATCGTTTAACCCCATTGCCTGGCGGATAAGTTGCGGCTTAACGCCAGGAAGCGTGTCGGCCAGTTTCAAACCaa                           <  1:4342443/93‑1 (MQ=255)

                                                              aaCGCAGCCATTCAGGCAAATCGTTTAACCCCATTGCCTGGCGGATAAGTTGCGGCTTAACGCCAGGAAGCGTGTCGGCCAGTTTCAAACCaa                           <  2:3847883/93‑1 (MQ=255)

                                                                cGCAGCCATTCAGGCAAATCGTTTAACCCCATTGCCTGGCGGATAAGTTGCGGCTTAACGCCAGGAAGCGTGTCGGCCAGTTTCAAACCAata                         <  1:5589921/93‑1 (MQ=255)

                                                                 gCAGCCATTCAGGCAAATCGTTTAACCCCATTGCCTGGCGGATAAGTTGCGGCTTAACGCCAGGAAGCGTGTCGGCCAGTTTCAAACCAatat                        <  1:373260/93‑1 (MQ=255)

                                                                 gCAGCCATTCAGGCAAATCGTTTAACCCCATTGCCTGGCGGATAAGTTGCGGCTTAACGCCAGGAAGCGTGTCGGCCAGTTTCAAACCAatat                        <  1:6565653/93‑1 (MQ=255)

                                                                 gCAGCCATTCAGGCAAATCGTTTAACCCCATTGCCTGGCGGATAAGTTGCGGCTTAACGCCAGGAAGCGTGTCGGCCAGTTTCAAACCAatat                        >  1:2862811/1‑93 (MQ=255)

                                                                 gCAGCCATTCAGGCAAATCGTTTAACCCCATTGCCTGGCGGATAAGTTGCGGCTTAACGCCAGGAAGCGTGTCGGCCAGTTTCAAACCAatat                        <  1:1385701/93‑1 (MQ=255)

                                                                   aGCCATTCAGGCAAATCGTTTAACCCCATTGCCTGGCGGATAAGTTGCGGCTTAACGCCAGGAAGCGTGTCGGCCAGTTTCAAACCAATATCa                      >  2:6513050/1‑93 (MQ=255)

                                                                   aGCCATTCAGGCAAATCGTTTAACCCCATTGCCTGGCGGATAAGTTGCGGCTTAACGCCAGGAAGCGTGTCGGCCAGTTTCAAACCAATATCa                      <  2:3957286/93‑1 (MQ=255)

                                                                    gCCATTCAGGCAAATCGTTTAACCCCATTGCCTGGCGGATAAGTTGCGGCTTAACGCCAGGAAGCGTGTCGGCCAGTTTCAAACCAATATCAc                     >  2:57062/1‑93 (MQ=255)

                                                                    gCCATTCAGGCAAATCGTTTAACCCCATTGCCTGGCGGATAAGTTGCGGCTTAACGCCAGGAAGCGTGTCGGCCAGTTTCAAACCAATATCAc                     >  1:6171858/1‑93 (MQ=255)

                                                                    gCCATTCAGGCAAATCGTTTAACCCCATTGCCTGGCGGATAAGTTGCGGCTTAACGCCAGGAAGCGTGTCGGCCAGTTTCAAACCAATATCAc                     >  1:6265912/1‑93 (MQ=255)

                                                                     ccATTCAGGCAAATCGTTTAACCCCATTGCCTGGCGGATAAGTTGCGGCTTAACGCCAGGAAGCGTGTCGGCCAGTTTCAAACCAATATCACg                    <  1:4468904/93‑1 (MQ=255)

                                                                     ccATTCAGGCAAATCGTTTAACCCCATTGCCTGGCGGATAAGTTGCGGCTTAACGCCAGGAAGCGTGTCGGCCAGTTTCAAACCAATATCACg                    <  2:3607743/93‑1 (MQ=255)

                                                                      cATTCAGGCAAATCGTTTAACCCCATTGCCTGGCGGATAAGTTGCGGCTTAACGCCAGGAAGCGTGTCGGCCAGTTTCAAACCAATATCACgc                   >  2:928741/1‑93 (MQ=255)

                                                                      cATTCAGGCAAATCGTTTAACCCCATTGCCTGGCGGATAAGTTGCGGCTTAACGCCAGGAAGCGTGTCGGCCAGTTTCAAACCAATATCACgc                   <  2:4410010/93‑1 (MQ=255)

                                                                       aTTCAGGCAAATCGTTTAACCCCATTGCCTGGCGGATAAGTTGCGGCTTAACGCCAGGAAGCGTGTCGGCCAGTTTCAAACCAATATCACgca                  >  2:171310/1‑93 (MQ=255)

                                                                       aTTCAGGCAAATCGTTTAACCCCATTGCCTGGCGGATAAGTTGCGGCTTAACGCCAGGAAGCGTGTCGGCCAGTTTCAAACCAATATCACgca                  <  1:6612852/93‑1 (MQ=255)

                                                                        ttCAGGCAAATCGTTTAACCCCATTGCCTGGCGGATAAGTTGCGGCTTAACGCCAGGAAGCGTGTCGGCCAGTTTCAAACCAATATCACgcag                 >  2:7607840/1‑93 (MQ=255)

                                                                        ttCAGGCAAATCGTTTAACCCCATTGCCTGGCGGATAAGTTGCGGCTTAACGCCAGGAAGCGTGTCGGCCAGTTTCAAACCAATATCACgcag                 >  1:2109485/1‑93 (MQ=255)

                                                                        ttCAGGCAAATCGTTTAACCCCATTGCCTGGCGGATAAGTTGCGGCTTAACGCCAGGAAGCGTGTCGGCCAGTTTCAAACCAATATCACgcag                 >  1:2429898/1‑93 (MQ=255)

                                                                        ttCAGGCAAATCGTTTAACCCCATTGCCTGGCGGATAAGTTGCGGCTTAACGCCAGGAAGCGTGTCGGCCAGTTTCAAACCAATATCACgcag                 >  1:3353099/1‑93 (MQ=255)

                                                                         tCAGGCAAATCGTTTAACCCCATTGCCTGGCGGATAAGTTGCGGCTTAACGCCAGGAAGCGTGTCGGCCAGTTTCAAACCAATATCACgcagc                <  1:1049940/93‑1 (MQ=255)

                                                                         tCAGGCAAATCGTTTAACCCCATTGCCTGGCGGATAAGTTGCGGCTTAACGCCAGGAAGCGTGTCGGCCAGTTTCAAACCAATATCACgcagc                >  1:7455951/1‑93 (MQ=255)

                                                                           aGGCAAATCGTTTAACCCCATTGCCTGGCGGATAAGTTGCGGCTTAACGCCAGGAAGCGTGTCGGCCAGTTTCAAACCAATATCACGcagcag              >  1:6406887/1‑93 (MQ=255)

                                                                           aGGCAAATCGTTTAACCCCATTGCCTGGCGGATAAGTTGCGGCTTAACGCCAGGAAGCGTGTCGGCCAGTTTCAAACCAATATCACGcagcag              >  1:160024/1‑93 (MQ=255)

                                                                           aGGCAAATCGTTTAACCCCATTGCCTGGCGGATAAGTTGCGGCTTAACGCCAGGAAGCGTGTCGGCCAGTTTCAAACCAATATCACGcagcag              >  2:7025772/1‑93 (MQ=255)

                                                                           aGGCAAATCGTTTAACCCCATTGCCTGGCGGATAAGTTGCGGCTTAACGCCAGGAAGCGTGTCGGCCAGTTTCAAACCAATATCACGcagcag              >  2:5537826/1‑93 (MQ=255)

                                                                            ggCAAATCGTTTAACCCCATTGCCTGGCGGATAAGTTGCGGCTTAACGCCAGGAAGCGTGTCGGCCAGTTTCAAACCAATATCACGCAGCAGt             >  2:5335554/1‑93 (MQ=255)

                                                                              cAAATCGTTTAACCCCATTGCCTGGCGGATAAGTTGCGGCTTAACGCCAGGAAGCGTGTCGGCCAGTTTCAAACCAATATCACGCAGCAGttt           <  2:1020852/93‑1 (MQ=255)

                                                                              cAAATCGTTTAACCCCATTGCCTGGCGGATAAGTTGCGGCTTAACGCCAGGAAGCGTGTCGGCCAGGTTCAAACCAATATCACGCAGCAGttt           >  1:1281790/1‑93 (MQ=255)

                                                                               aaaTCGTTTAACCCCATTGCCTGGCGGATAAGTTGCGGCTTAACGCCAGGAAGCGTGTCGGCCAGTTTCAAACCAATATCACGCAGCAGtttt          >  1:760094/1‑93 (MQ=255)

                                                                               aaaTCGTTTAACCCCATTGCCTGGCGGATAAGTTGCGGCTTAACGCCAGGAAGCGTGTCGGCCAGTTTCAAACCAATATCACGCAGCAGtttt          >  1:1040453/1‑93 (MQ=255)

                                                                                aaTCGTTTAACCCCATTGCCTGGCGGATAAGTTGCGGCTTAACGCCAGGAAGCGTGTCGGCCAGTTTCAAACCAATATCACGCAGCAGttttt         >  2:1241634/1‑93 (MQ=255)

                                                                                 aTCGTTTAACCCCATTGCCTGGCGGATAAGTTGCGGCTTAACGCCAGGAAGCGTGTCGGCCAGTTTCAAACCAATATCACGCAGCAGtttttt        >  2:6019463/1‑93 (MQ=255)

                                                                                 aTCGTTTAACCCCATTGCCTGGCGGATAAGTTGCGGCTTAACGCCAGGAAGCGTGTCGGCCAGTTTCAAACCAATATCACGCAGCAGtttttt        <  1:52950/93‑1 (MQ=255)

                                                                                  tCGTTTAACCCCATTGCCTGGCGGATAAGTTGCGGCTTAACGCCAGGAAGCGTGTCGGCCAGTTTCAAACCAATATCACGCAGCAGTTTTTTc       >  1:1992012/1‑93 (MQ=255)

                                                                                     tttAACCCCATTGCCTGGCGGATAAGTTGCGGCTTGACGCCAGGAAGCGTGTCGGCCAGTTTCAAACCAATATCACGCAGCAGTTTTTTCGcc    >  2:1546530/1‑93 (MQ=255)

                                                                                      ttAACCCCATTGCCTGGCGGATAAGTTGCGGCTTAACGCCAGGAAGCGTGTCGGCCAGTTTCAAACCAATATCACGCAGCAGTTTTTTCGCCg   <  2:1424685/93‑1 (MQ=255)

GCGAAAAAACCGGGAGATGAAAGTGTGATGGGTATCAAATAAACAACAGAGGAGAAATTTTTAACGCAGCCATTCAGGCAAATCGTTTAATCCCATTGCCTGGCGGATAAGTTGCGGCTTAACGCCAGGAAGCGTGTCGGCCAGTTTCAAACCAATATCACGCAGCAGTTTTTTCGCCGG  >  NC_000913/3049075‑3049254

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 31 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

CCGGCGAAAAAACCGGGAGATGAAAGTGTGATGGGTATCAAATAAACAACAGAGGAGAAATTTTTAACGCAGCCATTCAGGCAAATCGTTTAATCCCATTGCCTGGCGGATAAGTTGCGGCTTAACGCCAGGAAGCGTGTCGGCCAGTTTCAAACCAATATCACGCAGCAGTTTTTTCGCCGGATTG  >  NC_000913/3049072‑3049258

CCGGCGAAAAAACCGGGAGATGAAAGTGTGATGGGTATCAAATAAACAACAGAGGAGAAATTTTTAACGCAGCCATTCAGGCAAATCGTTTAAC                                                                                               >  SRR4302543.7797753/1‑94 (MQ=60)

 CGGCGAAAAAACCGGGAGATGAAAGTGTGATGGGTATCAAATAAACAACAGAGGAGAAATTTTTAACGCAGCCATTCAGGCAAATCGTTTAACC                                                                                              >  SRR4302543.5857513/1‑94 (MQ=60)

  GGCGAAAAAACCGGGAGATGAAAGTGTGATGGGTATCAAATAAACAACAGAGGAGAAATTTTTAACGCAGCCATTCAGGCAAATCGTTTAACCC                                                                                             >  SRR4302543.4943693/1‑94 (MQ=60)

  GGCGAAAAAACCGGGAGATGAAAGTGTGATGGGTATCAAATAAACAACAGAGGAGAAATTTTTAACGCAGCCATTCAGGCAAATCGTTTAACCC                                                                                             <  SRR4302543.6102738/94‑1 (MQ=60)

   GCGAAAAAACCGGGAGATGAAAGTGTGATGGGTATCAAATAAACAACAGAGGAGAAATTTTTAACGCAGCCATTCAGGCAAATCGTTTAACCCC                                                                                            <  SRR4302543.568913/94‑1 (MQ=60)

    CGAAAAAACCGGGAGATGAAAGCGTGATGAGTATCAAATAAACATCAAAGGAGACAGTTTTAACGCAACCACTCAGGCACATCGTTTAACCCCA                                                                                           >  SRR4302543.4025611/1‑94 (MQ=42)

      AAAAAACCGGGAGATGAAAGTGTGATGGGTATCAAATAAACAACAGAGGAGAAATTTTTAACGCAGCCATTCAGGCAAATCGTTTAACCCCATT                                                                                         <  SRR4302543.7581359/94‑1 (MQ=60)

       AAAAACCGGGAGATGAAAGTGTGATGGGTATCAAATAAACAACAGAGGAGAAATTTTTAACGCAGCCATTCAGGCAAATCGTTTAACCCCATTG                                                                                        <  SRR4302543.2802141/94‑1 (MQ=60)

       AAAAACCGGGAGATGAAAGTGTGATGGGTATCAAATAAACAACAGAGGAGAAATTTTTAACGCAGCCATTCAGGCAAATCGTTTAACCCCATTG                                                                                        <  SRR4302543.3217611/94‑1 (MQ=60)

       AAAAACCGGGAGATGAAAGTGTGATGGGTATCAAATAAACAACAGAGGAGAAATTTTTAACGCAGCCATTCAGGCAAATCGTTTAACCCCATTG                                                                                        <  SRR4302543.876109/94‑1 (MQ=60)

        AAAACCGGGAGATGAAAGTGTGATGGGTATCAAATAAACAACAGAGGAGAAATTTTTAACGCAGCCATTCAGGCAAATCGTTTAACCCCATTGC                                                                                       >  SRR4302543.2676250/1‑94 (MQ=60)

          AACCGGGAGATGAAAGTGTGATGGGTATCAAATAAACAACAGAGGAGAAATTTTTAACGCAGCCATTCAGGCAAATCGTTTAACCCCATTGCCT                                                                                     <  SRR4302543.7574705/94‑1 (MQ=60)

          AACCGGGAGATGAAAGTGTGATGGGTATCAAATAAACAACAGAGGAGAAATTTTTAACGCAGCCATTCAGGCAAATCGTTTAACCCCATTGCCT                                                                                     >  SRR4302543.3089834/1‑94 (MQ=60)

          AACCGGGAGATGAAAGTGTGATGGGTATCAAATAAACAACAGAGGAGAAATTTTTAACGCAGCCATTCAGGCAAATCGTTTAACCCCATTGCCT                                                                                     >  SRR4302543.5500307/1‑94 (MQ=60)

           ACCGGGAGATGAAAGTGTGATGGGTATCAAATAAACAACAGAGGAGAAATTTTTAACGCAGCCATTCAGGCAAATCGTTTAACCCCATTGCCTG                                                                                    <  SRR4302543.6125766/94‑1 (MQ=60)

           ACCGGGAGATGAAAGTGTGATGGGTATCAAATAAACAACAGAGGAGAAATTTTTAACGCAGCCATTCAGGCAAATCGTTTAACCCCATTGCCTG                                                                                    <  SRR4302543.6125767/94‑1 (MQ=60)

            CCGGGAGATGAAAGTGTGATGGGTATCAAATAAACAACAGAGGAGAAATTTTTAACGCAGCCATTCAGGCAAATCGTTTAACCCCATTGCCTGG                                                                                   <  SRR4302543.2252235/94‑1 (MQ=60)

            CCGGGAGATGAAAGTGTGATGGGTATCAAATAAACAACAGAGGAGAAATTTTTAACGCAGCCATTCAGGCAAATCGTTTAACCCCATTGCCTGG                                                                                   >  SRR4302543.1292028/1‑94 (MQ=60)

             CGGGAGATGAAAGTGTGATGGGTATCAAATAAACAACAGAGGAGAAATTTTTAACGCAGCCATTCAGGCAAATCGTTTAACCCCATTGCCTGGC                                                                                  >  SRR4302543.5012898/1‑94 (MQ=60)

              GGGAGATGAAAGTGTGATGGGTATCAAATAAACAACAGAGGAGAAATTTTTAACGCAGCCATTCAGGCAAATCGTTTAACCCCATTGCCTGGCG                                                                                 >  SRR4302543.6777705/1‑94 (MQ=60)

               GGAGATGAAAGTGTGATGGGTATCAAATAAACAACAGAGGAGAAATTTTTAACGCAGCCATTCAGGCAAATCGTTTAACCCCATTGCCTGGCGG                                                                                <  SRR4302543.7918568/94‑1 (MQ=60)

               GGAGATGAAAGTGTGATGGGTATCAAATAAACAACAGAGGAGAAATTTTTAACGCAGCCATTCAGGCAAATCGTTTAACCCCATTGCCTGGCGG                                                                                >  SRR4302543.4384333/1‑94 (MQ=60)

                 AGATGAAAGTGTGATGGGTATCAAATAAACAACAGAGGAGAAATTTTTAACGCAGCCATTCAGGCAAATCGTTTAACCCCATTGCCTGGCGGAT                                                                              >  SRR4302543.5193627/1‑94 (MQ=60)

                 AGATGAAAGTGTGATGGGTATCAAATAAACAACAGAGGAGAAATTTTTAACGCAGCCATTCAGGCAAATCGTTTAACCCCATTGCCTGGCGGAT                                                                              >  SRR4302543.6079985/1‑94 (MQ=60)

                   ATGAAAGTGTGATGGGTATCAAATAAACAACAGAGGAGAAATTTTTAACGCAGCCATTCAGGCAAATCGTTTAACCCCATTGCCTGGCGGATAA                                                                            >  SRR4302543.4710313/1‑94 (MQ=60)

                   ATGAAAGTGTGATGGGTATCAAATAAACAACAGAGGAGAAATTTTTAACGCAGCCATTCAGGCAAATCGTTTAACCCCATTGCCTGGCGGATAA                                                                            >  SRR4302543.105770/1‑94 (MQ=60)

                   ATGAAAGTGTGATGGGTATCAAATAAACAACAGAGGAGAAATTTTTAACGCAGCCATTCAGGCAAATCGTTTAACCCCATTGCCTGGCGGATAA                                                                            >  SRR4302543.6371277/1‑94 (MQ=60)

                   ATGAAAGTGTGATGGGTATCAAATAAACAACAGAGGAGAAATTTTTAACGCAGCCATTCAGGCAAATCGTTTAACCCCATTGCCTGGCGGATAA                                                                            <  SRR4302543.837180/94‑1 (MQ=60)

                   ATGAAAGTGTGATGGGTATCAAATAAACAACAGAGGAGAAATTTTTAACGCAGCCATTCAGGCAAATCGTTTAACCCCATTGCCTGGCGGATAA                                                                            <  SRR4302543.837179/94‑1 (MQ=60)

                    TGAAAGTGTGATGGGTATCAAATAAACAACAGAGGAGAAATTTTTAACGCAGCCATTCAGGCAAATCGTTTAACCCCATTGCCTGGCGGATAAG                                                                           >  SRR4302543.6885150/1‑94 (MQ=60)

                     GAAAGTGTGATGGGTATCAAATAAACAACAGAGGAGAAATTTTTAACGCAGCCATTCAGGCAAATCGTTTAACCCCATTGCCTGGCGGATAAGT                                                                          <  SRR4302543.7872138/94‑1 (MQ=60)

                       AAGTGTGATGGGTATCAAATAAACAACAGAGGAGAAATTTTTAACGCAGCCATTCAGGCAAATCGTTTAACCCCATTGCCTGGCGGATAAGTTG                                                                        <  SRR4302543.6299582/94‑1 (MQ=60)

                         GTGTGATGGGTATCAAATAAACAACAGAGGAGAAATTTTTAACGCAGCCATTCAGGCAAATCGTTTAACCCCATTGCCTGGCGGATAAGTTGCG                                                                      <  SRR4302543.1322049/94‑1 (MQ=60)

                         GTGTGATGGGTATCAAATAAACAACAGAGGAGAAATTTTTAACGCAGCCATTCAGGCAAATCGTTTAACCCCATTGCCTGGCGGATAAGTTGCG                                                                      <  SRR4302543.7572551/94‑1 (MQ=60)

                         GTGTGATGGGTATCAAATAAACAACAGAGGAGAAATTTTTAACGCAGCCATTCAGGCAAATCGTTTAACCCCATTGCCTGGCGGATAAGTTGCG                                                                      <  SRR4302543.2191590/94‑1 (MQ=60)

                          TGTGATGGGTATCAAATAAACAACAGAGGAGAAATTTTTAACGCAGCCATTCAGGCAAATCGTTTAACCCCATTGCCTGGCGGATAAGTTGCGG                                                                     >  SRR4302543.5991347/1‑94 (MQ=60)

                            TGATGGGTATCAAATAAACAACAGAGGAGAAATTTTTAACGCAGCCATTCAGGCAAATCGTTTAACCCCATTGCCTGGCGGATAAGTTGCGGCT                                                                   >  SRR4302543.5029704/1‑94 (MQ=60)

                            TGATGGGTATCAAATAAACAACAGAGGAGAAATTTTTAACGCAGCCATTCAGGCAAATCGTTTAACCCCATTGCCTGGCGGATAAGTTGCGGCT                                                                   >  SRR4302543.4781023/1‑94 (MQ=60)

                             GATGGGTATCAAATAAACAACAGAGGAGAAATTTTTAACGCAGCCATTCAGGCAAATCGTTTAACCCCATTGCCTGGCGGATAAGTTGCGGCTT                                                                  >  SRR4302543.4211859/1‑94 (MQ=60)

                               TGGGTATCAAATAAACAACAGAGGAGAAATTTTTAACGCAGCCATTCAGGCAAATCGTTTAACCCCATTGCCTGGCGGATAAGTTGCGGCTTAA                                                                >  SRR4302543.2953416/1‑94 (MQ=60)

                                GGGTATCAAATAAACAACAGAGGAGAAATTTTTAACGCAGCCATTCAGGCAAATCGTTTAACCCCATTGCCTGGCGGATAAGTTGCGGCTTAAC                                                               <  SRR4302543.3781535/94‑1 (MQ=60)

                                GGGTATCAAATAAACAACAGAGGAGAAATTTTTAACGCAGCCATTCAGGCAAATCGTTTAACCCCATTGCCTGGCGGATAAGTTGCGGCTTAAC                                                               <  SRR4302543.5132695/94‑1 (MQ=60)

                                 GGTATCAAATAAGCAACAGAGGAGAAATTTTTAACGCAGCCATTCAGGCAAATCGTTTAACCCCATTGCCTGGCGGATAAGTTGCGGCTTAACG                                                              <  SRR4302543.5004489/94‑1 (MQ=60)

                                 GGTATCAAATAAACAACAGAGGAGAAATTTTTAACGCAGCCATTCAGGCAAATCGTTTAATCCCATTGCCTGGCGGCTAAGTTGAGGCTTAATG                                                              >  SRR4302543.6750110/1‑94 (MQ=60)

                                 GGTATCAAATAAACAACAGAGGAGAAATTTTTAACGCAGCCATTCAGGCAAATCGTTTAACCCCATTGCCTGGCGGATAAGTTGCGGCTTAACG                                                              >  SRR4302543.4032936/1‑94 (MQ=60)

                                 GGTATCAAATAAACAACAGAGGAGAAATTTTTAACGCAGCCATTCAGGCAAATCGTTTAACCCCATTGCCTGGCGGATAAGTTGCGGCTTAACG                                                              >  SRR4302543.592721/1‑94 (MQ=60)

                                  GTATCAAATAAACAACAGAGGAGAAATTTTTAACGCAGCCATTCAGGCAAATCGTTTAACCCCATTGCCTGGCGGATAAGTTGCGGCTTAACGC                                                             >  SRR4302543.2004778/1‑94 (MQ=60)

                                  GTATCAAATAAACAACAGAGGAGAAATTTTTAACGCAGCCATTCAGGCAAATCGTTTAACCCCATTGCCTGGCGGATAAGTTGCGGCTTAACGC                                                             <  SRR4302543.185318/94‑1 (MQ=60)

                                      CAAATAAACAACAGAGGAGAAATTTTTAACGCAGCCATTCAGGCAAATCGTTTAACCCCATTGCCTGGCGGATAAGTTGCGGCTTAACGCCAGG                                                         >  SRR4302543.7476527/1‑94 (MQ=60)

                                      CAAATAAACAACAGAGGAGAAATTTTTAACGCAGCCATTCAGGCAAATCGTTTAACCCCATTGCCTGGCGGATAAGTTGCGGCTTAACGCCAGG                                                         <  SRR4302543.2384766/94‑1 (MQ=60)

                                       AAATAAACAACAGAGGAGAAATTTTTAACGCAGCCATTCAGGCAAATCGTTTAACCCCATTGCCTGGCGGATAAGTTGCGGCTTAACGCCAGGA                                                        >  SRR4302543.4100941/1‑94 (MQ=60)

                                        AATAAACAACAGAGGAGAAATTTTTAACGCAGCCATTCAGGCAAATCGTTTAACCCCATTGCCTGGCGGATAAGTTGCGGCTTAACGCCAGGAA                                                       <  SRR4302543.2231915/94‑1 (MQ=60)

                                         ATAAACAACAGAGGAGAAATTTTTAACGCAGCCATTCAGGCAAATCGTTTAACCCCATTGCCTGGCGGATAAGTTGCGGCTTAACGCCAGGAAG                                                      <  SRR4302543.3559330/94‑1 (MQ=60)

                                         ATAAACAACAGAGGAGAAATTTTTAACGCAGCCATTCAGGCAAATCGTTTAACCCCATTGCCTGGCGGATAAGTTGCGGCTTAACGCCAGGAAG                                                      <  SRR4302543.5116804/94‑1 (MQ=60)

                                         ATAAACAACAGAGGAGAAATTTTTAACGCAGCCATTCAGGCAAATCGTTTAACCCCATTGCCTGGCGGATAAGTTGCGGCTTAACGCCAGGAAG                                                      >  SRR4302543.1943194/1‑94 (MQ=60)

                                          TAAACAACAGAGGAGAAATTTTTAACGCAGCCATTCAGGCAAATCGTTTAACCCCATTGCCTGGCGGATAAGTTGCGGCTTAACGCCAGGAAGC                                                     <  SRR4302543.6276510/94‑1 (MQ=60)

                                          TAAACAACAGAGGAGAAATTTTTAACGCAGCCATTCAGGCAAATCGTTTAACCCCATTGCCTGGCGGATAAGTTGCGGCTTAACGCCAGGAAGC                                                     <  SRR4302543.3781081/94‑1 (MQ=60)

                                           AAACAACAGAGGAGAAATTTTTAACGCAGCCATTCAGGCAAATCGTTTAACCCCATTGCCTGGCGGATAAGTTGCGGCTTAACGCCAGGAAGCG                                                    >  SRR4302543.3593432/1‑94 (MQ=60)

                                           AAACAACAGAGGAGAAATTTTTAACGCAGCCATTCAGGCAAATCGTTTAACCCCATTGCCTGGCGGATAAGTTGCGGCTTAACGCCAGGAAGCG                                                    >  SRR4302543.279415/1‑94 (MQ=60)

                                            GACAACAGAGGAGAAATTTTTAACGCAGCCATTCAGGCAAATCGTTTAACCCCATTGCCTGGCGGATAAGTTGCGGCTTAACGCCAGGAAGCGT                                                   <  SRR4302543.496279/94‑1 (MQ=60)

                                             ACAACAGAGGAGAAATTTTTAACGCAGCCATTCAGGCAAATCGTTTAACCCCATTGCCTGGCGGATAAGTTGCGGCTTAACGCCAGGAAGCGTG                                                  <  SRR4302543.2345779/94‑1 (MQ=60)

                                              CAACAGAGGAGAAATTTTTAACGCAGCCATTCAGGCAAATCGTTTAACCCCATTGCCTGGCGGATAAGTTGCGGCTTAACGCCAGGAAGCGTGT                                                 <  SRR4302543.7023177/94‑1 (MQ=60)

                                              CAACAGAGGAGAAATTTTTAACGCAGCCATTCAGGCAAATCGTTTAACCCCATTGCCTGGCGGATAAGTTGCGGCTTAACGCCAGGAAGCGTGT                                                 <  SRR4302543.6644879/94‑1 (MQ=60)

                                               AACAGAGGAGAAATTTTTAACGCAGCCATTCAGGCAAATCGTTTAACCCCATTGCCTGGCGGATAAGTTGCGGCTTAACGCCAGGAAGCGTGTC                                                <  SRR4302543.6783441/94‑1 (MQ=60)

                                                ACAGAGGAGAAATTTTTAACGCAGCCATTCAGGCAAATCGTTTAACCCCATTGCCTGGCGGATAAGTTGCGGCTTAACGCCAGGAAGCGTGTCG                                               <  SRR4302543.2089268/94‑1 (MQ=60)

                                                ACAGAGGAGAAATTTTTAACGCAGCCATTCAGGCAAATCGTTTAACCCCATTGCCTGGCGGATAAGTTGCGGCTTAACGCCAGGAAGCGTGTCG                                               >  SRR4302543.6483041/1‑94 (MQ=60)

                                                 CAGAGGAGAAATTTTTAACGCAGCCATTCAGGCAAATCGTTTAACCCCATTGCCTGGCGGATAAGTTGCGGCTTAACGCCAGGAAGCGTGTCGG                                              >  SRR4302543.5453073/1‑94 (MQ=60)

                                                   GAGGAGAAATTTTTAACGCAGCCATTCAGGCAAATCGTTTAACCCCATTGCCTGGCGGATAAGTTGCGGCTTAACGCCAGGAAGCGTGTCGGCC                                            <  SRR4302543.995474/94‑1 (MQ=60)

                                                    AGGAGAAATTTTTAACGCAGCCATTCAGGCAAATCGTTTAACCCCATTGCCTGGCGGATAAGTTGCGGCTTAACGCCAGGAAGCGTGTCGGCGA                                           >  SRR4302543.73088/1‑94 (MQ=60)

                                                     GGAGAAATTTTTAACGCAGCCATTCAGGCAAATCGTTTAACCCCATTGCCTGGCGGATAAGTTGCGGCTTAACGCCAGGAAGCGTGTCGGCCAG                                          <  SRR4302543.5957990/94‑1 (MQ=60)

                                                     GGAGAAATTTTTAACGCAGCCATTCAGGCAAATCGTTTAACCCCATTGCCTGGCGGATAAGTTGCGGCTTAACGCCAGGAAGCGTGTCGGCCAG                                          <  SRR4302543.7532051/94‑1 (MQ=60)

                                                     GGAGAAATTTTTAACGCAGCCATTCAGGCAAATCGTTTAACCCCATTGCCTGGCGGATAAGTTGCGGCTTAACGCCAGGAAGCGTGTCGGCCAG                                          <  SRR4302543.1958/94‑1 (MQ=60)

                                                       AGAAATTTTTAACGCAGCCATTCAGGCAAATCGTTTAACCCCATTGCCTGGCGGATAAGTTGCGGCTTAACGCCAGGAAGCGTGTCGGCCAGTT                                        >  SRR4302543.3846907/1‑94 (MQ=60)

                                                           ATTTTTAACGCAGCCATTCAGGCAAATCGTTTAACCCCATTGCCTGGCGGATAAGTTGCGGCTTAACGCCAGGAAGCGTGTCGGCCAGTTTCAA                                    >  SRR4302543.2364919/1‑94 (MQ=60)

                                                             TTTTAACGCAGCCATTCAGGCAAATCGTTTAACCCCATTGCCTGGCGGATAAGTTGCGGCTTAACGCCAGGAAGCGTGTCGGCCAGTTTCAAAC                                  >  SRR4302543.1214924/1‑94 (MQ=60)

                                                             TTTTAACGCAGCCATTCAGGCAAATCGTTTAACCCCATTGCCTGGCGGATAAG‑TGCGGCTTAACGCCAGGAAGCGGGTCGGCCAGTTTCAAACC                                 >  SRR4302543.1679160/1‑94 (MQ=60)

                                                              TTTAACGCAGCCATTCAGGCAAATCGTTTAACCCCATTGCCTGGCGGATAAGTTGCGGCTTAACGCCAGGAAGCGTGTCGGCCAGTTTCAAACC                                 >  SRR4302543.5221496/1‑94 (MQ=60)

                                                               TTAACGCAGCCATTCAGGCAAATCGTTTAACCCCATTGCCTGGCGGATAAGTTGCGGCTTAACGCCAGGAAGCGTGTCGGCCAGTTTCAAACCA                                <  SRR4302543.4907989/94‑1 (MQ=60)

                                                               TTAACGCAGCCATTCAGGCAAATCGTTTAACCCCATTGCCTGGCGGATAAGTTGCGGCTTAACGCCAGGAAGCGTGTCGGCCAGTTTCAAACCA                                <  SRR4302543.6059209/94‑1 (MQ=60)

                                                                 AACGCAGCCATTCAGGCAAATCGTTTAACCCCATTGCCTGGCGGATAAGTTGCGGCTTAACGCCAGGAAGCGTGTCGGCCAGTTTCAAACCAAT                              <  SRR4302543.4381825/94‑1 (MQ=60)

                                                                  ACGCAGCCATTCAGGCAAATCGTTTAACCCCATTGCCTGGCGGATAAGTTGCGGCTTAACGCCAGGAAGCGTGTCGGCCAGTTTCAAACCAATA                             <  SRR4302543.4622832/94‑1 (MQ=60)

                                                                   CGCAGCCATTCAGGCAAATCGTTTAACCCCATTGCCTGGCGGATAAGTTGCGGCTTACCGCCAGGAAGCGTGTCGGCCAGTTTCAAACCAATAT                            <  SRR4302543.3724027/94‑1 (MQ=60)

                                                                   CGCAGCCATTCAGGCAAATCGTTTAACCCCATTGCCTGGCGGATAAGTTGCGGCTTAACGCCAGGAAGCGTGTCGGCCAGTTTCAAACCAATAT                            >  SRR4302543.2495699/1‑94 (MQ=60)

                                                                   CGCAGCCATTCAGGCAAATCGTTTAACCCCATTGCCTGGCGGATAAGTTGCGGCTTAACGCCAGGAAGCGTGTCGGCCAGTTTCAAACCAATAT                            >  SRR4302543.2888913/1‑94 (MQ=60)

                                                                   CGCAGCCATTCAGGCAAATCGTTTAACCCCATTGCCTGGCGGATAAGTTGCGGCTTAACGCCAGGAAGCGTGTCGGCCAGTTTCAAACCAATAT                            <  SRR4302543.5640840/94‑1 (MQ=60)

                                                                    GCAGCCATTCAGGCAAATCGTTTAACCCCATTGCCTGGCGGATAAGTTGCGGCTTAACGCCAGGAAGCGTGTCGGCCAGTTTCAAACCAATATC                           <  SRR4302543.6627434/94‑1 (MQ=60)

                                                                    GCAGCCATTCAGGCAAATCGTTTAACCCCATTGCCTGGCGGATAAGTTGCGGCTTAACGCCAGGAAGCGTGTCGGCCAGTTTCAAACCAATATC                           <  SRR4302543.1398169/94‑1 (MQ=60)

                                                                    GCAGCCATTCAGGCAAATCGTTTAACCCCATTGCCTGGCGGATAAGTTGCGGCTTAACGCCAGGAAGCGTGTCGGCCAGTTTCAAACCAATATC                           <  SRR4302543.376699/94‑1 (MQ=60)

                                                                      AGCCATTCAGGCAAATCGTTTAACCCCATTGCCTGGCGGATAAGTTGCGGCTTAACGCCAGGAAGCGTGTCGGCCAGTTTCAAACCAATATCAC                         >  SRR4302543.1425141/1‑94 (MQ=60)

                                                                      AGCCATTCAGGCAAATCGTTTAACCCCATTGCCTGGCGGATAAGTTGCGGCTTAACGCCAGGAAGCGTGTCGGCCAGTTTCAAACCAATATCAC                         >  SRR4302543.6324992/1‑94 (MQ=60)

                                                                      AGCCATTCAGGCAAATCGTTTAACCCCATTGCCTGGCGGATAAGTTGCGGCTTAACGCCAGGAAGCGTGTCGGCCAGTTTCAAACCAATATCAC                         >  SRR4302543.6230235/1‑94 (MQ=60)

                                                                       GCCATTCAGGCAAATCGTTTAACCCCATTGCCTGGCGGATAAGTTGCGGCTTAACGCCAGGAAGCGTGTCGGCCAGTTTCAAACCAATATCACC                        >  SRR4302543.2688725/1‑94 (MQ=60)

                                                                        CCATTCAGGCAAATCGTTTAACCCCATTGCCTGGCGGATAAGTTGCGGCTTAACGCCAGGAAGCGTGTCGGCCAGTTTCAAACCAATATCACGC                       <  SRR4302543.4509429/94‑1 (MQ=60)

                                                                        CCATTCAGGCAAATCGTTTAACCCCATTGCCTGGCGGATAAGTTGCGGCTTAACGCCAGGAAGCGTGTCGGCCAGTTTCAAACCAATATCACGC                       <  SRR4302543.4509428/94‑1 (MQ=60)

                                                                         CATTCAGGCAAATCGTTTAACCCCATTGCCTGGCGGATAAGTTGCGGCTTAACGCCAGGAAGCGTGTCGGCCAGTTTCAAACCAATATCACGCA                      <  SRR4302543.1529080/94‑1 (MQ=60)

                                                                          ATTCAGGCAAATCGTTTAACCCCATTGCCTGGCGGATAAGTTGCGGCTTAACGCCAGGAAGCGTGTCGGCCAGTTTCAAACCAATATCACGCAG                     >  SRR4302543.2451826/1‑94 (MQ=60)

                                                                          ATTCAGGCAAATCGTTTAACCCCATTGCCTGGCGGATAAGTTGCGGCTTAACGCCAGGAAGCGTGTCGGCCAGTTTCAAACCAATATCACGCAG                     >  SRR4302543.3383283/1‑94 (MQ=60)

                                                                          ATTCAGGCAAATCGTTTAACCCCATTGCCTGGCGGATAAGTTGCGGCTTAACGCCAGGAAGCGTGTCGGCCAGTTTCAAACCAATATCACGCAG                     >  SRR4302543.2128366/1‑94 (MQ=60)

                                                                          ATTCAGGCAAATCGTTTAACCCCATTGCCTGGCGGATAAGTTGCGGCTTAACGCCAGGAAGCGTGTCGGCCAGTTTCAAACCAATATCACGCAG                     <  SRR4302543.6675191/94‑1 (MQ=60)

                                                                          ATTCAGGCAAATCGTTTAACCCCATTGCCTGGCGGATAAGTTGCGGCTTAACGCCAGGAAGCGTGTCGGCCAGTTTCAAACCAATATCACGCAG                     >  SRR4302543.6524315/1‑94 (MQ=60)

                                                                           TTCAGGCAAATCGTTTAACCCCATTGCCTGGCGGATAAGTTGCGGCTTAACGCCAGGAAGCGTGTCGGCCAGTTTCAAACCAATATCACGCAGC                    >  SRR4302543.7527379/1‑94 (MQ=60)

                                                                            TCAGGCAAATCGTTTAACCCCATTGCCTGGCGGATAAGTTGCGGCTTAACGCCAGGAAGCGTGTCGGCCAGTTTCAAACCAATATCACGCAGCA                   >  SRR4302543.2996427/1‑94 (MQ=60)

                                                                            TCAGGCAAATCGTTTAACCCCATTGCCTGGCGGATAAGTTGCGGCTTAACGCCAGGAAGCGTGTCGGCCAGTTTCAAACCAATATCACGCAGCA                   <  SRR4302543.992206/94‑1 (MQ=60)

                                                                            TCAGGCAAATCGTTTAACCCCATTGCCTGGCGGATAAGTTGCGGCTTAACGCCAGGAAGCGTGTCGGCCAGTTTCAAACCAATATCACGCAGCA                   <  SRR4302543.1059345/94‑1 (MQ=60)

                                                                            TCAGGCAAATCGTTTAACCCCATTGCCTGGCGGATAAGTTGCGGCTTAACGCCAGGAAGCGTGTCGGCCAGTTTCAAACCAATATCACGCAGCA                   <  SRR4302543.1218142/94‑1 (MQ=60)

                                                                             CAGGCAAATCGTTTAACCCCATTGCCTTGCGGATAAGTTGCGGCTTAACGCCAGGAAGCGTGTCGGCCAGTTTCAAACCAAGATCACGCAGCAG                  <  SRR4302543.2187806/94‑1 (MQ=60)

                                                                             CAGGCAAATCGTTTAACCCCATTGCCTGGCGGATAAGTTGCGGCTTAACGCCAGGAAGCGTGTCGGCCAGTTTCAAACCAATATCACGCAGCAG                  >  SRR4302543.6467224/1‑94 (MQ=60)

                                                                             CAGGCAAATCGTTTAACCCCATTGCCTGGCGGATAAGTTGCGGCTTAACGCCAGGAAGCGTGTCGGCCAGTTTCAAACCAATATCACGCAGCAG                  >  SRR4302543.812405/1‑94 (MQ=60)

                                                                             CAGGCAAATCGTTTAACCCCATTGCCTGGCGGATAAGTTGCGGCTTAACGCCAGGAAGCGTGTCGGCCAGTTTCAAACCAATATCACGCAGCAG                  >  SRR4302543.161392/1‑94 (MQ=60)

                                                                              AGGCAAATCGTTTAACCCCATTGCCTGGCGGATAAGTTGCGGCTTAACGCCAGGAAGCGTGTCGGCCAGTTTCAAACCAATATCACGCAGCAGT                 <  SRR4302543.2646473/94‑1 (MQ=60)

                                                                              AGGCAAATCGTTTAACCCCATTGCCTGGCGGATAAGTTGCGGCTTAACGCCAGGAAGCGTGTCGGCCAGTTTCAAACCAATATCACGCAGCAGT                 >  SRR4302543.7945506/1‑94 (MQ=60)

                                                                               GGCAAATCGTTTAACCCCATTGCCTGGCGGATAAGTTGCGGCTTAACGCCAGGAAGCGTGTCGGCCAGTTTCAAACCAATATCACGCAGCAGTT                >  SRR4302543.1139237/1‑94 (MQ=60)

                                                                                GCAAATCGTTTAACCCCATTGCCTGGCGGATAAGTTGCGGCTTAACGCCAGGAAGCGTGTCGGCCAGGTTCAAACCAATATCACGCAGCAGTTT               >  SRR4302543.1293221/1‑94 (MQ=60)

                                                                                 CAAATCGTTTAACCCCATTGCCTGGCGGATAAGTTGCGGCTTAACGCCAGGAAGCGTGTCGGCCAGTTTCAAACCAATATCACGCAGCAGTTTT              >  SRR4302543.766804/1‑94 (MQ=60)

                                                                                 CAAATCGTTTAACCCCATTGCCTGGCGGATAAGTTGCGGCTTAACGCCAGGAAGCGTGTCGGCCAGTTTCAAACCAATATCACGCAGCAGTTTT              >  SRR4302543.1049722/1‑94 (MQ=60)

                                                                                 CAAATCGTTTAACCCCATTGCCTGGCGGATAAGTTGCGGCTTAACGCCAGGAAGCGTGTCGGCCAGTTTCAAACCAATATCACGCAGCAGTTTT              <  SRR4302543.1680275/94‑1 (MQ=60)

                                                                                  AAATCGTTTAACCCCATTGCCTGGCGGATAAGTTGCGGCTTAACGCCAGGAAGCGTGTCGGCCAGTTTCAAACCAATATCACGCAGCAGTTTTT             >  SRR4302543.2355223/1‑94 (MQ=60)

                                                                                  AAATCGTTTAACCCCATTGCCTGGCGGATAAGTTGCGGCTTAACGCCAGGAAGCGTGTCGGCCAGCTTCAAACCAATATCACGCAGCAGCTTTT             >  SRR4302543.2355222/1‑94 (MQ=60)

                                                                                   AATCGTTTAACCCCATTGCCTGGCGGATAAGTTGCGGCTTAACGCCAGGAAGCGTGTCGGCCAGTTTCAAACCAATATCACGCAGCAGTTTTTT            <  SRR4302543.1682289/94‑1 (MQ=60)

                                                                                    ATCGTTTAACCCCATTGCCTGGCGGATAAGTTGCGGCTTAACGCCAGGAAGCGTGTCGGCCAGTTTCAAACCAATATCACGCAGCAGTTTTTTC           >  SRR4302543.3476659/1‑94 (MQ=60)

                                                                                    ATCGTTTAACCCCATTGCCTGGCGGATAAGTTGCGGCTTAACGCCAGGAAGCGTGTCGGCCAGTTTCAAACCAATATCACGCAGCAGTTTTTTC           >  SRR4302543.3476658/1‑94 (MQ=60)

                                                                                    ATCGTTTAACCCCATTGCCTGGCGGATAAGTTGCGGCTTAACGCCAGGAAGCGTGTCGGCCAGTTTCAAACCAATATCACGCAGCAGTTTTTTC           >  SRR4302543.2009873/1‑94 (MQ=60)

                                                                                    ATCGTTTAACCCCATTGCCTGGCGGATAAGTTGCGGCTTAACGCCAGGAAGCGTGTCGGCCAGTTTCAAACCAATATCACGCAGCAGTTTTTTC           <  SRR4302543.53419/94‑1 (MQ=60)

                                                                                      CGTTTAACCCCATTGCCTGGCGGATAAGTTGCGGCTTAACGCCAGGAAGCGTGTCGGCCAGTTTCAAACCAATATCACGCAGCAGTTTTTTCGC         >  SRR4302543.4112297/1‑94 (MQ=60)

                                                                                       GTTTAACCCCATTGCCTGGCGGATAAGTTGCGGCTTAACGCCAGGAAGCGTGTCGGCCAGTTTCAAACCAATATCACGCAGCAGTTTTTTCGCC        <  SRR4302543.1676827/94‑1 (MQ=60)

                                                                                          TAACCCCATTGCCTGGCGGATAAGTTGCGGCTTAACGCCAGGAAGCGTGTCGGCCAGTTTCAAACCAATATCACGCAGCAGTTTTTTCGCCGGA     <  SRR4302543.2006931/94‑1 (MQ=60)

                                                                                          TAACCCCATTGCCTGGCGGATAAGTTGCGGCTTAACGCCAGGAAGCGTGTCGGCCAGTTTCAAACCAATATCACGCAGCAGTTTTTTCGCCGGA     >  SRR4302543.3568055/1‑94 (MQ=60)

                                                                                          TAACCCCATTGCCTGGCGGATAAGTTGCGGCTTAACGCCAGGAAGCGTGTCGGCCAGTTTCAAACCAATATCACGCAGCAGTTTTTTCGCCGGA     >  SRR4302543.4745282/1‑94 (MQ=60)

                                                                                           AACCCCATTGCCTGGCGGATAAGTTGCGGCTTAACGCCAGGAAGCGTGTCGGCCAGTTTCAAACCAATATCACGCAGCAGTTTTTTCGCCGGAT    <  SRR4302543.6935187/94‑1 (MQ=60)

                                                                                           AACCCCATTGCCTGGCGGATAAGTTGCGGCTTAACGCCAGGAAGCGTGTCGGCCAGTTTCAAACCAATATCACGCAGCAGTTTTTTCGCCGGAT    >  SRR4302543.967682/1‑94 (MQ=60)

                                                                                           AACCCCATTGCCTGGCGGATAAGTTGCGGCTTAACGCCAGGAAGCGTGTCGGCCAGTTTCAAACCAATATCACGCAGCAGTTTTTTCGCCGGAT    >  SRR4302543.4739640/1‑94 (MQ=60)

                                                                                             CCCCATTGCCTGGCGGATAAGTTGCGGCTTAACGCCAGGAAGCGTGTCGGCCAGTTTCAAACCAATATCACGCAGCAGTTTTTTCGCCGGATTG  >  SRR4302543.7929733/1‑94 (MQ=60)

                                                                                             CCCCATTGCCTGGCGGATAAGTTGCGGCTTAACGCCAGGAAGCGTGTCGGCCAGTTTCAAACCAATATCACGCAGCAGTTTTTTCGCCGGATTG  >  SRR4302543.2088259/1‑94 (MQ=60)

                                                                                             CCCCATTGCCTGGCGGATAAGTTGCGGCTTAACGCCAGGAAGCGTGTCGGCCAGTTTCAAACCAATATCACGCAGCAGTTTTTTCGCCGGATTG  >  SRR4302543.1670277/1‑94 (MQ=60)

CCGGCGAAAAAACCGGGAGATGAAAGTGTGATGGGTATCAAATAAACAACAGAGGAGAAATTTTTAACGCAGCCATTCAGGCAAATCGTTTAATCCCATTGCCTGGCGGATAAGTTGCGGCTTAACGCCAGGAAGCGTGTCGGCCAGTTTCAAACCAATATCACGCAGCAGTTTTTTCGCCGGATTG  >  NC_000913/3049072‑3049258

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`