Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 1696 279.3 15874187 97.8% 15524954 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,082,961 A→G S324P (TCC→CCC)  speA ← biosynthetic arginine decarboxylase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,082,9610AG99.3% 907.9 / ‑4.7 296S324P (TCC→CCC) speAbiosynthetic arginine decarboxylase
Reads supporting (aligned to +/- strand):  ref base A (1/1);  new base G (168/126);  total (169/127)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.96e-01

Only 100 of 305 total aligned reads displayed.

GCGGCAGACCGTTTTCTTCACACGCATCGCCAATCGCCCAGATAATGTTGTTGGCGTATTCATTGAGGCCGTAGTTCACCGAACAGTCGGACTGCGAACGAGTACCTTCATAATCCACGCCCAGACCGCCGCCGACGTCGAAGCACTGAATATTGACGCCCAGCTTGTGCAGTTCCACA  >  NC_000913/3082871‑3083049
                                                                                          |                                                                                        
 cGGCAGACCGTTTTCTTCACACGCATCGCCAATCGCCCAGATAATGTTGTTGGCGTATTCATTGAGGCCGTAGTTCACCGAACAGTCGGGCTg                                                                                       >  2:1661624/1‑93 (MQ=255)
 cGGCAGACCGTTTTCTTCACACGCATCGCCAATCGCCCAGATAATGTTGTTGGCGTATTCATTGAGGCCGTAGTTCACCGAACAGTCGGGCTg                                                                                       <  1:5360336/93‑1 (MQ=255)
  ggCAGACCGTTTTCTTCACACGCATCGCCAATCGCCCAGATAATGTTGTTGGCGTATTCATTGAGGCCGTAGTTCACCGAACAGTCGGGCTGc                                                                                      >  1:5724507/1‑93 (MQ=255)
   gCAGACCGTTTTCTTCACACGCATCGCCAATCGCCCAGATAATGTTGTTGGCGTATTCATTGAGGCCGTAGTTCACCGAACAGTCGGGCTGCg                                                                                     >  2:3122150/1‑93 (MQ=255)
    cAGACCGTTTTCTTCACACGCATCGCCAATCGCCCAGATAATGTTGTTGGCGTATTCATTGAGGCCGTAGTTCACCGAACAGTCGGGCTGCGa                                                                                    >  1:4117796/1‑93 (MQ=255)
     aGACCGTTTTCTTCACACGCATCGCCAATCGCCCAGATAATGTTGTTGGCGTATTCATTGAGGCCGTAGTTCACCGAACAGTCGGGCTGCGaa                                                                                   >  1:3076567/1‑93 (MQ=255)
     aGACCGTTTTCTTCACACGCATCGCCAATCGCCCAGATAATGTTGTTGGCGTATTCATTGAGGCCGTAGTTCACCGAACAGTCGGGCTGCGaa                                                                                   <  1:6064269/93‑1 (MQ=255)
         cGTTTTCTTCACACGCATCGCCAATCGCCCAGATAATGTTGTTGGCGTATTCATTGAGGCCGTAGTTCACCGAACAGTCGGGCTGCGAACGAg                                                                               >  2:3758574/1‑93 (MQ=255)
          gTTTTCTTCACACGCATCGCCAATCGCCCAGATAATGTTGTTGGCGTATTCATTGAGGCCGTAGTTCACCGAACAGTCGGGCTGCGAACGAGt                                                                              >  1:289318/1‑93 (MQ=255)
          gTTTTCTTCACACGCATCGCCAATCGCCCAGATAATGTTGTTGGCGTATTCATTGACGCCGTAGTTCACCGAACAGTCGGGATGCGAACGAga                                                                              >  1:2464511/1‑92 (MQ=255)
           ttttCTTCACACGCATCGCCAATCGCCCAGATAATGTTGTTGGCGTATTCATTGAGGCCGTAGTTCACCGAACAGTCGGGCTGCGAACGAGTa                                                                             >  2:2801245/1‑93 (MQ=255)
           gtttCTTCACACGCATCGCCAATCGCCCAGATAATGTTGTTGGCGTATTCATTGAGGCCGTAGTTCACCGAACAGTCGGGCTGCGAACGAGTa                                                                             <  1:2331524/92‑1 (MQ=255)
            tttCTTCACACGCATCGCCAATCGCCCAGATAATGTTGTTGGCGTATTCATTGAGGCCGTAGTTCACCGAACAGTCGGGCTGCGAACGAGTAc                                                                            >  1:493992/1‑93 (MQ=255)
             ttcttcACACGCATCGCCAATCGCCCAGATAATGTTGTTGGCGTATTCATTGAGGCCGTAGTTCACCGAACAGTCGGGCTGCGAACGAGTAcc                                                                           >  1:2617944/1‑93 (MQ=255)
               cttcACACGCATCGCCAATCGCCCAGATAATGTTGTTGGCGTATTCATTGAGGCCGTAGTTCACCGAACAGTCGGACTGCGAACGAGTACCtt                                                                         >  1:6267882/1‑93 (MQ=255)
                ttcACACGCATCGCCAAGCGCCCAGATAATGTTGTTGGCGTATTCATTGAGGCCGTAGTTCACCGAACACTCGGGCTCCGAACGAGTACCTTc                                                                        <  2:3686100/93‑1 (MQ=255)
                   acacGCACCGCCAATCGCCCAGATAATGTTGTTGGCGTATTCATTGAGGCCGTAGTTCACCGAACAGTCGGGCTGCGAACGAGTACCTTCATa                                                                     <  1:7301734/93‑1 (MQ=255)
                    cacGCATCGCCAATCGCCCAGATAATGTTGTTGGCGTATTCATTGAGGCCGTAGTTCACCGAACAGTCGGGCTGCGAACGAGTACCTTCATaa                                                                    >  2:1978826/1‑93 (MQ=255)
                    cacGCATCGCCAATCGCCCAGATAATGTTGTTGGCGTATTCATTGAGGCCGTAGTTCACCGAACAGTCGGGCTGCGAACGAGTACCTTCATaa                                                                    <  1:1837393/93‑1 (MQ=255)
                     acGCATCGCCAATCGCCCAGATAATGTTGTTGGCGTATTCATTGAGGCCGTAGTTCACCGAACAGTCGGGCTGCGAACGAGTACCTTCATAAt                                                                   >  2:4971171/1‑93 (MQ=255)
                     acGCATCGCCAATCGCCCAGATAATGTTGTTGGCGTATTCATTGAGGCCGTAGTTCACCGAACAGTCGGGCTGCGAACGAGTACCTTCATAAt                                                                   >  1:1859266/1‑93 (MQ=255)
                        cATCGCCAATCGCCCAGATAATGTTGTTGGCGTATTCATTGAGGCCGTAGTTCACCGAACAGTCGGGCTGCGAACGAGTACCTTCATAATCCa                                                                <  2:6611419/93‑1 (MQ=255)
                         aTCGCCAATCGCCCAGATAATGTTGTTGGCGTATTCATTGAGGCCGTAGTTCACCGAACAGTCGGGCTGCGAACGAGTACCTTCATAATCCAc                                                               >  1:6853041/1‑93 (MQ=255)
                          tCGCCAATCGCCCAGATAATGTTGTTGGCGTATTCATTGAGGCCGTAGTTCACCGAACAGTCGGGCTGCGAACGAGTACCTTCATAATCCACg                                                              >  2:4840097/1‑93 (MQ=255)
                            gCCAATCGCCCAGATAATGTTGTTGGCGTATTCATTGAGGCCGTAGTTCACCGAACAGTCGGGCTTCGAACGAGTACCTTCATAATCCACGcc                                                            >  2:7688262/1‑93 (MQ=255)
                             ccAATCGCCCAGATAATGTTGTTGGCGTATTCATTGAGGCCGTAGTTCACCGAACAGTCGGGCTGCGAACGAGTACCTTCATAATCCACGccc                                                           <  2:5802679/93‑1 (MQ=255)
                              cAATCGCCCAGATAATGTTGTTGGCGTATTCATTGAGGCCGTAGTTCACCGAACAGTCGGGCTGCGAACGAGTACCTTCATAATCCACGCCCa                                                          >  1:7917770/1‑93 (MQ=255)
                              cAATCGCCCAGATAATGTTGTTGGCGTATTCATTGAGGCCGTAGTTCACCGAACAGTCGGGCTGCGAACGAGTACCTTCATAATCCACGCCCa                                                          >  1:4623914/1‑93 (MQ=255)
                              cAATCGCCCAGATAATGTTGTTGGCGTATTCATTGAGGCCGTAGTTCACCGAACAGTCGGGCTGCGAACGAGTACCTTCATAATCCACGCCCa                                                          <  2:3051023/93‑1 (MQ=255)
                              cAATCGCCCAGATAATGTTGTTGGCGTATTCATTGAGGCCGTAGTTCACCGAACAGTCGGGCTGCGAACGAGTACCTTCATAATCCACGCCCa                                                          >  1:5168296/1‑93 (MQ=255)
                               aaTCGCCCAGATAATGTTGTTGGCGTATTCATTGAGGCCGTAGTTCACCGAACAGTCGGGCTGCGAACGAGTACCTTCATAATCCACGCCCAg                                                         >  1:616618/1‑93 (MQ=255)
                               aaTCGCCCAGATAATGTTGTTGGCGTATTCATTGAGGCCGTAGTTCACCGAACAGTCGGGCTGCGAACGAGTACCTTCATAATCCACGCCCAg                                                         >  1:822521/1‑93 (MQ=255)
                                aTCGCCCAGATAATGTTGTTGGCGTATTCATTGAGGCCGTAGTTCACCGAACAGTCGGGCTGCGAACGAGTACCTTCATAATCCACGCCCAGa                                                        <  1:1557567/93‑1 (MQ=255)
                                aTCGCCCAGATAATGTTGTTGGCGTATTCATTGAGGCCGTAGTTCACCGAACAGTCGGGCTGCGAACGAGTACCTTCATAATCCACGCCCAGa                                                        <  1:7558123/93‑1 (MQ=255)
                                 tCGCCCAGATAATGTTGTTGGCGTATTCATTGAGGCCGTAGTTCACCGAACAGTCGGGCTGCGAACGAGTACCTTCATAATCCACGCCCAGAc                                                       >  2:3463154/1‑93 (MQ=255)
                                    cccAGATAATGTTGTTGGCGTATTCATTGAGGCCGTAGTTCACCGAACAGTCGGGCTGCGAACGAGTACCTTCATAATCCACGCCCAGAccgc                                                    >  2:1366178/1‑93 (MQ=255)
                                     ccAGATAATGTTGTTGGCGTATTCATTGAGGCCGTAGTTCACCGAACAGTCGGGCTGCGAACGAGTACCTTCATAATCCACGCCCAGAccgcc                                                   <  2:7090891/93‑1 (MQ=255)
                                      cAGATAATGTTGTTGGCGTATTCATTGAGGCCGTAGTTCACCGAACAGTCGGGCTGCGAACGAGTACCTTCATAATCCACGCCCAGAccgccg                                                  >  2:1161886/1‑93 (MQ=255)
                                      cAGATAATGTTGTTGGCGTATTCATTGAGGCCGTAGTTCACCGAACAGTCGGGCTGCGAACGAGTACCTTCATAATCCACGCCCAGAccgccg                                                  >  2:6875032/1‑93 (MQ=255)
                                       aGATAATGTTGTTGGCGTATTCATTGAGGCCGTAGTTCACCGAACAGTCGGGCTGCGAACGAGTACCTTCATAATCCACGCCCAGAccgccgc                                                 <  2:6954775/93‑1 (MQ=255)
                                       aGATAATGTTGTTGGCGTATTCATTGAGGCCGTAGTTCACCGAACAGTCGGGCTGCGAACGAGTACCTTCATAATCCACGCCCAGAccgccgc                                                 <  1:2161150/93‑1 (MQ=255)
                                        gATAATGTTGTTGGCGTATTCATTGAGGCCGTAGTTCACCGAACAGTCGGGCTGCGAACGAGTACCTTCATAATCCACGCCCAGAccgccgcc                                                >  2:2544501/1‑93 (MQ=255)
                                         aTAATGTTGTTGGCGTATTCATTGAGGCCGTAGTTCACCGAACAGTCGGGCTGCGAACGAGTACCTTCATAATCCACGCCCAGAccgccgccg                                               <  2:2381045/93‑1 (MQ=255)
                                         aTAATGTTGTTGGCGTATTCATTGAGGCCGTAGTTCACCGAACAGTCGGGCTGCGAACGAGTACCTTCATAATCCACGCCCAGAccgccgccg                                               >  2:5014819/1‑93 (MQ=255)
                                          tAATGTTGTTGGCGTATTCATTGAGGCCGTAGTTCACCGAACAGTCGGGCTGCGAACGAGTACCTTCATAATCCACGCCCAGAccgccgccgc                                              >  1:3643102/1‑92 (MQ=255)
                                          tAATGTTGTTGGCGTATTCATTGAGGCCGTAGTTCACCGAACAGTCGGGCTGCGAACGAGTACCTTCATAATCCACGCCCAGACCGCCGCCGa                                              <  2:7315634/93‑1 (MQ=255)
                                          tAATGTTGTTGGCGTATTCATTGAGGCCGTAGTTCACCGAACAGTCGGGCTGCGAACGAGTACCTTCATAATCCACGCCCAGACCGCCGCCGa                                              <  1:1217380/93‑1 (MQ=255)
                                            aTGTTGTTGGCGTATTCATTGAGGCCGTAGTTCACCGAACAGTCGGGCTGCGAACGAGTACCTTCATAATCCACGCCCAGACCGCCGCCGACg                                            >  2:2157082/1‑93 (MQ=255)
                                             tgttgtTGGCGTATTCATTGAGGCCGTAGTTCACCGAACAGTCGGGCTGCGAACGAGTACCTTCATAATCCACGCCCAGATCGCCGCCGACGt                                           >  2:7912499/1‑93 (MQ=255)
                                             tgttgtTGGCGTATTCATTGAGGCCGTAGTTCACCGAACAGTCGGGCTGCGAACGAGTACCTTCATAATCCACGCCCAGACCGCCGCCGACGt                                           <  2:4769526/93‑1 (MQ=255)
                                              gttgttGGCGTATTCATTGAGGCCGTAGTTCACCGAACAGTCGGGCTGCGAACGAGTACCTTCATAATCCACGCCCAGACCGCCGCCGACGTc                                          >  2:6719263/1‑93 (MQ=255)
                                               ttgttgGCGTATTCATTGAGGCCGTAGTTCACCGAACAGTCGGGCTGCGAACGAGTACCTTCATAATCCACGCCCAGACCGCCGCCGACGTCg                                         <  2:6551913/93‑1 (MQ=255)
                                                  ttgGCGTATTCATTGAGGCCGTAGTTCACCGAACAGTCGGGCTGCGAACGAGTACCTTCATAATCCACGCCCAGACCGCCGCCGACGCCGAAg                                      >  1:295456/1‑93 (MQ=255)
                                                   tgGCGTATTCATTGAGGCCGTAGTTCACCGAACAGTCGGGCTGCGAACGAGTACCTTCATAATCCACGCCCAGACCGCCGCCGACGTCGAAGc                                     >  2:5908253/1‑93 (MQ=255)
                                                   tgGCGTATTCATTGAGGCCGTAGTTCACCGAACAGTCGGGCTGCGAACGAGTACCTTCATAATCCACGCCCAGACCGCCGCCGACGTCGAAGc                                     >  1:878683/1‑93 (MQ=255)
                                                    ggCGTATTCATTGAGTCCGGAGGTCACCGAACAGTCGGACTGCGAACGAGTACCTTCATAATCCACGCCCAGACCGCCGCCGACGTCGAAGCa                                    <  1:4198777/93‑1 (MQ=255)
                                                     gCGTATTCATTGAGGCCGTAGTTCACCGAACAGTCGGGCTGCGAACGAGTACCTTCATAATCCACGCCCAGACCGCCGCCGACGTCGAAGCAc                                   <  2:2884024/93‑1 (MQ=255)
                                                      cGTATTCATTGAGGCCGTAGTTCACCGAACAGTCGGGCTGCGAACGAGTACCTTCATAATCCACGCCCAGACCGCCGCCGACGTCGAAGCACt                                  <  2:4052154/93‑1 (MQ=255)
                                                      cGTATTCATTGAGGCCGTAGTTCACCGAACAGTCGGGCTGCGAACGAGTACCTTCATAATCCACGCCCAGACCGCCGCCGACGTCGAAGCACt                                  <  2:1524451/93‑1 (MQ=255)
                                                        tATTCATTGAGGCCGTAGTTCACCGAACAGTCGGGCTGCGAACGAGTACCTTCATAATCCACGCCCAGACCGCCGCCGACGTCGAAGCACTGa                                >  1:6592614/1‑93 (MQ=255)
                                                        tATTCATTGAGGCCGTAGTTCACCGAACAGTCGGGCTGCGAACGAGTACCTTCATAATCCACGCCCAGACCGCCGCCGACGTCGAAGCACTGa                                >  2:1747805/1‑93 (MQ=255)
                                                        tATTCATTGAGGCCGTAGTTCACCGAACAGTCGGGCTGCGAACGAGTACCTTCATAATCCACGCCCAGACCGCCGCCGACGTCGAAGCACTGa                                >  1:2737636/1‑93 (MQ=255)
                                                        tATTCATTGAGGCCGTAGTTCACCAAACAGTCGGGCTGCGAACGAGTACCTTCATAATCCACGCCCAGACCGCCGCCGACGTCGAAGCACTGa                                >  1:1407098/1‑93 (MQ=255)
                                                         aTTCATTGAGGCCGTAGTTCACCGAACAGTCGGGCTGCGAACGAGTACCTTCATAATCCACGCCCAGACCGCCGCCGACGTCGAAGCACTGaa                               >  1:5981262/1‑93 (MQ=255)
                                                         aTTCATTGAGGCCGTAGTTCACCGAACAGTCGGGCTGCGAACGAGTACCTTCATAATCCACGCCCAGACCGCCGCCGACGTCGAAGCACTGaa                               <  2:4159666/93‑1 (MQ=255)
                                                         aTTCATTGAGGCCGTAGTTCACCGAACAGTCGGGCTGCGAACGAGTACCTTCATAATCCACGCCCAGACCGCCGCCGACGTCGAAGCACTGaa                               <  1:3316004/93‑1 (MQ=255)
                                                             aTTGAGGCCGTAGTTCACCGAACAGTCGGGCTGCGAACGAGTACCTTCATAATCCACGCCCAGACCGGCGCCGACGTCGAAGCACTGAATAtt                           >  2:5614831/1‑93 (MQ=255)
                                                             aTTGAGGCCGTAGTTCACCGAACAGTCGGGCTGCGAACGAGTACCTTCATAATCCACGCCCAGACCGCCGCCGACGTCGAAGCACTGAATAtt                           >  1:5792698/1‑93 (MQ=255)
                                                             aTTGAGGCCGTAGTTCACCGAACAGTCGGGCTGCGAACGAGTACCTTCATAATCCACGCCCAGACCGCCGCCGACGTCGAAGCACTGAATAtt                           >  2:3330743/1‑93 (MQ=255)
                                                             aTTGAGGCCGTAGTTCACCGAACAGTCGGGCTGCGAACGAGTACCTTCATAATCCACGCCCAGACCGCCGCCGACGTCGAAGCACTGAATAtt                           >  1:1029177/1‑93 (MQ=255)
                                                              ttGAGGCCGTAGTTCACCGAACAGTCGGGCTGCGAACGAGTACCTTCATAATCCACGCCCAGACCGCCGCCGACGTCGAAGCACTGAATATTg                          >  2:5727736/1‑93 (MQ=255)
                                                              ttGAGGCCGTAGTTCACCGAACAGTCGGGCTGCGAACGAGTACCTTCATAATCCACGCCCAGACCGCCGCCGACGTCGAAGCACTGAATATTg                          >  1:1384607/1‑93 (MQ=255)
                                                              ttGAGGCCGTAGTTCACCGAACAGTCGGGCTGCGAACGAGTACCTTCATAATCCACGCCCAGACCGCCGCCGACGTCGAAGCACTGAATATTg                          >  2:617295/1‑93 (MQ=255)
                                                               tGAGGCCGTAGTTCACCGAACAGTCGGGCTGCGAACGAGTACCTTCATAATCCACGCCCAGACCGCCGCCGACGTCGAAGCACTGAATATTGa                         <  2:745431/93‑1 (MQ=255)
                                                               tGAGGCCGTAGTTCACCGAACAGTCGGGCTGCGAACGAGTACCTTCATAATCCACGCCCAGACCGCCGCCGACGTCGAAGCACTGAATATTGa                         <  1:3532619/93‑1 (MQ=255)
                                                               tGAGGCCGTAGTTCACCGAACAGTCGGGCTGCGAACGAGTACCTTCATAATCCACGCCCAGACCGCCGCCGACGTCGAAGCACTGAATATTGa                         <  1:284373/93‑1 (MQ=255)
                                                               tGAGGCCGTAGTTCACCGAACAGTCGGGCTGCGAACGAGTACCTTCATAATCCACGCCCAGACCGCCGCCGACGTCGAAGCACTGAATATTGa                         <  1:7826638/93‑1 (MQ=255)
                                                               tGAGGCCGTAGTTCACCGAACAGTCGGGCTGCGAACGAGTACCTTCATAATCCACGCCCAGACCGCCGCCGACGTCGAAGCACTGAATATTGa                         <  2:5095245/93‑1 (MQ=255)
                                                               tGAGGCCGTAGTTCACCGAACAGTCGGGCTGCGAACGAGTACCTTCATAATACACGCCCAGACCGCCGCCGACGTCGAAGCACTGAATATTGa                         <  1:5252960/93‑1 (MQ=255)
                                                                   gCCGTAGTTCACCGAACAGTCGGGCTGCGAACGAGTACCTTCATAATCCACGCCCAGACCGCCGCCGACGTCGAAGCACTGAATATTGACGcc                     >  2:954774/1‑93 (MQ=255)
                                                                   gCCGTAGTTCACCGAACAGTCGGGCTGCGAACGAGTACCTTCATAATCCACGCCCAGACCGCCGCCGACGTCGAAGCACTGAATATTGACGcc                     <  1:1502858/93‑1 (MQ=255)
                                                                      gTAGTTCACCGAACAGTCGGGCTGCGAACGAGTACCTTCATAATCCACGCCCAGACCGCCGCCGACGTCGAAGCACTGAATATTGACGCCCAg                  >  2:5203796/1‑93 (MQ=255)
                                                                      gTAGTTCACCGAACAGTCGGGCTGCGAACGAGTACCTTCATAATCCACGCCCAGACCGCCGCCGACGTCGAAGCACTGAATATTGACGCCCAg                  <  1:32093/93‑1 (MQ=255)
                                                                      gTAGTTCACCGAACAGTCGGGCTGCGAACGAGTACCTTCATAATCCACGCCCAGACCGCCGCCGACGTCGAAGCACTGAATATTGACGCCCAg                  >  2:7214390/1‑93 (MQ=255)
                                                                       tAGTTCACCGAACAGTCGGGCTGCGAACGAGTACCTTCATAATCCACGCCCAGACCGCCGCCGACGTCGAAGCACTGAATATTGACGCCCAGc                 >  2:4422795/1‑93 (MQ=255)
                                                                          ttCACCGAACAGTCGGGCTGCGACCGAGTACCTTCATAATCCACGCCCAGACCGCCGCCGACGTCGAAGCACTGAATATTGACGCCCAGCTtg              <  2:4298911/93‑1 (MQ=255)
                                                                          ttCACCGAACAGTCGGGCTGCGAACGAGTACCTTCATAATCCACGCCCAGACCGCCGCCGACGTCGAAGCACTGGATATTGACGCCCAGCttt              >  2:3387154/1‑92 (MQ=255)
                                                                             aCCGAACAGTCGGGCTGCGAACGAGTACCTTCATAATCCACGCCCAGACCGCCGCCGACGTCGAAGCACTGAATATTGACGCCCAGCTTGTGc           >  2:6771922/1‑93 (MQ=255)
                                                                              ccGAACAGTCGGGCTGCGAACGAGTACCTTCATAATCCACGCCCAGACCGCCGCCGACGTCGAAGCACTGAATATTGACGCCCAGCTTGTGCa          <  1:774657/93‑1 (MQ=255)
                                                                              ccGAACAGTCGGGCTGCGAACGAGTACCTTCATAATCCACGCCCAGACCGCCGCCGACGTCGAAGCACTGAATATTGACGCCCAGCTTGTGCa          <  2:1213087/93‑1 (MQ=255)
                                                                              ccGAACAGACGGGCTGCGAACGAGTACCTTCATAATCCACGCCCAGACCGCCGCCGACGTCGCAGCACTGAATATTGACGCCCAGCTTGTGCa          >  1:917140/1‑93 (MQ=255)
                                                                               cGAACAGTCGGGCTGCGAACGAGTACCTTCATAATCCACGCCCAGACCGCCGCCGACGTCGAAGCACTGAATATTGACGCCCAGCTTGTGCAg         >  1:6111415/1‑93 (MQ=255)
                                                                               cGAACAGTCGGGCTGCGAACGAGTACCTTCATAATCCACGCCCAGACCGCCGCCGACGTCGAAGCACTGAATATTGACGCCCAGCTTGTGCAg         >  1:3883120/1‑93 (MQ=255)
                                                                                  aCAGTCGGGCTGCGAACGAGTACCTTCATAATCCACGCCCAGACCGCCGCCGACGTCGAAGCACTGAATATTGACGCCCAGCTTGTGCAGTTc      <  1:7119147/93‑1 (MQ=255)
                                                                                  aCAGTCGGGCTGCGAACGAGTACCTTCATAATCCACGCCCAGACCGCCGCCGACGTCGAAGCACTGAATATTGACGCCCAGCTTGTGCAGTTc      <  1:4500250/93‑1 (MQ=255)
                                                                                    aGTCGGGCTGCGAACGAGTACCTTCATAATCCACGCCCAGACCGCCGCCGACGTCGAAGCACTGAATATTGACGCCCAGCTTGTGCAGTTCca    <  1:5055491/93‑1 (MQ=255)
                                                                                    aGTCGGGCTGCGAACGAGTACCTTCATAATCCACGCCCAGACCGCCGCCGACGTCGAAGCACTGAATATTGACGCCCAGCTTGTGCAGTTCca    <  2:7515516/93‑1 (MQ=255)
                                                                                     gTCGGGCTGCGAACGAGTACCTTCATAATCCACGCCCAGACCGCCGCCGACGTCGAAGCACTGAATATTGACGCCCAGCTTGTGCAGTTCcac   >  2:6313003/1‑93 (MQ=255)
                                                                                     gTCGGGCTGCGAACGAGTACCTTCATAATCCACGCCCAGACCGCCGCCGACGTCGAAGCACTGAATATTGACGCCCAGCTTGTGCAGTTCcac   <  1:5559542/93‑1 (MQ=255)
                                                                                      tCGGGCTGCGAACGAGTACCTTCATAATCCACGCCCAGACCGCCGCCGACGTCGAAGCACTGAATATTGACGCCCAGCTTGTGCAGTTCcaca  <  1:1959350/93‑1 (MQ=255)
                                                                                          |                                                                                        
GCGGCAGACCGTTTTCTTCACACGCATCGCCAATCGCCCAGATAATGTTGTTGGCGTATTCATTGAGGCCGTAGTTCACCGAACAGTCGGACTGCGAACGAGTACCTTCATAATCCACGCCCAGACCGCCGCCGACGTCGAAGCACTGAATATTGACGCCCAGCTTGTGCAGTTCCACA  >  NC_000913/3082871‑3083049

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
ATGCGGCAGACCGTTTTCTTCACACGCATCGCCAATCGCCCAGATAATGTTGTTGGCGTATTCATTGAGGCCGTAGTTCACCGAACAGTCGGACTGCGAACGAGTACCTTCATAATCCACGCCCAGACCGCCGCCGACGTCGAAGCACTGAATATTGACGCCCAGCTTGTGCAGTTCCACATAGAA  >  NC_000913/3082869‑3083054
                                                                                            |                                                                                             
ATGCGGCAGACCGTTTTCTTCACACGCATCGCCAATCGCCCAGATAATGTTGTTGGCGTATTCATTGAGGCCGTAGTTCACCGAACAGTCGGGC                                                                                              >  SRR4302543.6906452/1‑94 (MQ=60)
ATGCGGCAGACCGTTTTCTTCACACGCATCGCCAATCGCCCAGATAATGTTGTTGGCGTATTCATTGAGGCCGTAGTTCACCGAACAGTCGGGC                                                                                              >  SRR4302543.4363109/1‑94 (MQ=60)
ATGCGGCAGACCGTTTTCTTCACACGCATCGCCAATCGCCCAGATAATGTTGTTGGCGTATTCATTGAGGCCGTAGTTCACCGAACAGTCGGGC                                                                                              >  SRR4302543.7970060/1‑94 (MQ=60)
   CGGCAGACCGTTTTCTTCACACGCATCGCCAATCGCCCAGATAATGTTGTTGGCGTATTCATTGAGGCCGTAGTTCACCGAACAGTCGGGCTGC                                                                                           >  SRR4302543.5776743/1‑94 (MQ=60)
   CGGCAGACCGTTTTCTTCACACGCATCGCCAATCGCCCAGATAATGTTGTTGGCGTATTCATTGAGGCCGTAGTTCACCGAACAGTCGGGCTGC                                                                                           <  SRR4302543.2088050/94‑1 (MQ=60)
   CGGCAGACCGTTTTCTTCACACGCATCGCCAATCGCCCAGATAATGTTGTTGGCGTATTCATTGAGGCCGTAGTTCACCGAACAGTCGGGCTGC                                                                                           <  SRR4302543.5408847/94‑1 (MQ=60)
     GCAGACCGTTTTCTTCACACGCATCGCCAATCGCCCAGATAATGTTGTTGGCGTATTCATTGAGGCCGTAGTTCACCGAACAGTCGGGCTGCGA                                                                                         >  SRR4302543.4154892/1‑94 (MQ=60)
      CAGACCGTTTTCTTCACACGCATCGCCAATCGCCCAGATAATGTTGTTGGCGTATTCATTGAGGCCGTAGTTCACCGAACAGTCGGGCTGCGAA                                                                                        >  SRR4302543.822089/1‑94 (MQ=60)
      CAGACCGTTTTCTTCACACGCATCGCCAATCGCCCAGATAATGTTGTTGGCGTATTCATTGAGGCCGTAGTTCACCGAACAGTCGGGCTGCGAA                                                                                        >  SRR4302543.801551/1‑94 (MQ=60)
      CAGACCGTTTTCTTCACACGCATCGCCAATCGCCCAGATAATGTTGTTGGCGTATTCATTGAGGCCGTAGTTCACCGAACAGTCGGGCTGCGAA                                                                                        >  SRR4302543.3104237/1‑94 (MQ=60)
       AGACCGTTTTCTTCACACGCATCGCCAATCGCCCAGATAATGTTGTTGGCGTATTCATTGAGGCCGTAGTTCACCGAACAGTCGGGCTGCGAAC                                                                                       <  SRR4302543.6791338/94‑1 (MQ=60)
       AGACCGTTTTCTTCACACGCATCGCCAATCGCCCAGATAATGTTGTTGGCGTATTCATTGAGGCCGTAGTTCACCGAACAGTCGGGCTGCGAAC                                                                                       <  SRR4302543.6121737/94‑1 (MQ=60)
        GACCGTTTTCTTCACACGCATCGCCAATCGCCCAGATAATGTTGTTGGCGTATTCATTGAGGCCGTAGTTCACCGAACAGTCGGGCTGCGAACG                                                                                      >  SRR4302543.2886660/1‑94 (MQ=60)
        GACCGTTTTCTTCACACGCATCGCCAATCGCCCAGATAATGTTGTTGGCGTATTCATTGAGGCCGTAGTTCACCGAACAGTCGGGCTGCGAACG                                                                                      <  SRR4302543.2605664/94‑1 (MQ=60)
           CGTTTTCTTCACACGCATCGCCAATCGCCCAGATAATGTTGTTGGCGTATTCATTGAGGCCGTAGTTCACCGAACAGTCGGGCTGCGAACGAGT                                                                                   >  SRR4302543.291899/1‑94 (MQ=60)
           CGTTTTCTTCACACGCATCGCCAATCGCCCAGATAATGTTGTTGGCGTATTCATTGAGGCCGTAGTTCACCGAACAGTCGGGCTGCGAACGAGA                                                                                   >  SRR4302543.5782034/1‑94 (MQ=60)
           CGTTTTCTTCACACGCATCGCCAATCGCCCAGATAATGTTGTTGGCGTATTCATTGACGCCGTAGTTCACCGAACAGTCGGGATGCGAACGAGA                                                                                   >  SRR4302543.2486744/1‑94 (MQ=60)
            GTTTTCTTCACACGCATCGCCAATCGCCCAGATAATGTTGTTGGCGTATTCATTGAGGCCGTAGTTCACCGAACAGTCGGGCTGCGAACGAGTA                                                                                  >  SRR4302543.6330459/1‑94 (MQ=60)
            GTTTTCTTCACACGCATCGCCAATCGCCCAGATAATGTTGTTGGCGTATTCATTGAGGCCGTAGTTCACCGAACAGTCGGGCTGCGAACGAGTA                                                                                  >  SRR4302543.259890/1‑94 (MQ=60)
            CTTTTCTTCACACGCATCGCCAATCGCCCAGATAATGTTGTTGGCGTATTCATTGAGGCCGTAGTTCACCGAACAGTCGGGCTGCGAACGAGTA                                                                                  <  SRR4302543.6416814/94‑1 (MQ=60)
             TTTTCTTCACACGCATCGCCAATCGCCCAGATAATGTTGTTGGCGTATTCATTGAGGCCGTAGTTCACCGAACAGTCGGGCTGCGAACGAGTAC                                                                                 >  SRR4302543.1403022/1‑94 (MQ=60)
             TTTTCTTCACACGCATCGCCAATCGCCCAGATAATGTTGTTGGCGTATTCATTGAGGCCGTAGTTCACCGAACAGTCGGGCTGCGAACGAGTAC                                                                                 >  SRR4302543.498563/1‑94 (MQ=60)
             GTTTCTTCACACGCATCGCCAATCGCCCAGATAATGTTGTTGGCGTATTCATTGAGGCCGTAGTTCACCGAACAGTCGGGCTGCGAACGAGTAC                                                                                 <  SRR4302543.2352687/94‑1 (MQ=60)
              TTTCTTCACACGCATCGCCAATCGCCCAGATAATGTTGTTGGCGTATTCATTGAGGTCGTAGTTCACCGAACAGTCGGGCTGCGAACGAGTACC                                                                                >  SRR4302543.2360855/1‑94 (MQ=60)
              TTTCTTCACACGCATCGCCAATCGCCCAGATAATGTTGTTGGCGTATTCATTGAGGCCGTAGTTCACCGAACAGTCGGGCTGCGAACGAGTACC                                                                                >  SRR4302543.7436626/1‑94 (MQ=60)
              TTTCTTCACACGCATCGCCAATCGCCCAGATAATGTTGTTGGCGTATTCATTGAGGCCGTAGTTCACCGAACAGTCGGGCTGCGAACGAGTACC                                                                                >  SRR4302543.2641678/1‑94 (MQ=60)
                TCTTCACACGCATCGCCAATCGCCCAGATAATGTTGTTGGCGTATTCATTGAGGCCGTAGTTCACCGAACAGTCGGACTGCGAACGAGTACCTT                                                                              >  SRR4302543.6326984/1‑94 (MQ=60)
                 CTTCACACGCATCGCCAATCGCCCAGATAATGTTGTTGGCGTATTCATTGAGGCCGTAGTTCACCGAACAGTCGGGCTGCGAACGAGTACCTTC                                                                             >  SRR4302543.5565417/1‑94 (MQ=60)
                 CTTCACACGCATCGCCAATCGCCCAGATAATGTTGTTGGCGTATTCATTGAGGCCGTAGTTCACCGAACAGTCGGGCTGCGAACGAGTACCTTC                                                                             >  SRR4302543.1453933/1‑94 (MQ=60)
                  TTCACACGCATCGCCAATCGCCCAGATAATGTTGTTGGCGTATTCATTGAGGCCGTAGTTCACCGAACAGTCGGGCTGCGAACGAGTACCTTCA                                                                            >  SRR4302543.7185173/1‑94 (MQ=60)
                   TCACACGCATCGCCAATCGCCCAGATAATGTTGTTGGCGTATTCATTGAGGCCGTAGTTCACCGAACAGTCGGGCTGCGAACGAGTACCTTCAT                                                                           <  SRR4302543.2767627/94‑1 (MQ=60)
                   TCACACGCATCGCCAATCGCCCAGATAATGTTGTTGGCGTATTCATTGAGGCCGTAGTTCACCGAACAGTCGGGCTGCGAACGAGTACCTTCAT                                                                           <  SRR4302543.2311689/94‑1 (MQ=60)
                    CACACGCATCGCCAATCGCCCAGATAATGTTGTTGGCGTATTCATTGAGGCCGTAGTTCACCGAACAGTCGGGCTGCGAACGAGTACCTTCATA                                                                          <  SRR4302543.5723214/94‑1 (MQ=60)
                     ACACGCATCGCCAATCGCCCAGATAATGTTGTTGGCGTATTCATTGAGGCCGTAGTTCACCGAACAGTCGGGCTGCGAACGAGTACCTTCATAA                                                                         <  SRR4302543.1875847/94‑1 (MQ=60)
                     ACACGCATCGCCAATCGCCCAGATAATGTTGTTGGCGTATTCATTGAGGCCGTAGTTCACCGAACAGTCGGGCTGCGAACGAGTACCTTCATAA                                                                         >  SRR4302543.4200147/1‑94 (MQ=60)
                     ACACGCACCGCCAATCGCCCAGATAATGTTGTTGGCGTATTCATTGAGGCCGTAGTTCACCGAACAGTCGGGCTGCGAACGAGTACCTTCATAA                                                                         <  SRR4302543.7372161/94‑1 (MQ=60)
                      CACGCATCGCCAATCGCCCAGATAATGTTGTTGGCGTATTCATTGAGGCCGTAGTTCACCGAACAGTCGGGCTGCGAACGAGTACCTTCATAAT                                                                        >  SRR4302543.1875973/1‑94 (MQ=60)
                      CACGCATCGCCAATCGCCCAGATAATGTTGTTGGCGTATTCATTGAGGCCGTAGTTCACCGAACAGTCGGGCTGCGAACGAGTACCTTCATAAT                                                                        <  SRR4302543.1853922/94‑1 (MQ=60)
                      CACGCATCGCCAATCGCCCAGATAATGTTGTTGGCGTATTCATTGAGGCCGTAGTTCACCGAACAGTCGGGCTGCGAACGAGTACCTTCATAAT                                                                        >  SRR4302543.5206138/1‑94 (MQ=60)
                      CACGCATCGCCAATCGCCCAGATAATGTTGTTGGCGTATTCATTGAGGCCGTAGTTCACCGAACAGTCGGGCTGCGAACGAGTACCTTCATAAT                                                                        <  SRR4302543.6118723/94‑1 (MQ=60)
                       ACGCATCGCCAATCGCCCAGATAATGTTGTTGGCGTATTCATTGAGGCCGTAGTTCACCGAACAGTCGGGCTGCGAACGAGTACCTTCATAATC                                                                       <  SRR4302543.2750587/94‑1 (MQ=60)
                        CGCATCGCCAATCGCCCAGATAATGTTGTTGGCGTATTCATTGAGGCCGTAGTTCACCGAACAGTCGGGCTGCGAACGAGTACCTTCATAATCC                                                                      >  SRR4302543.4060674/1‑94 (MQ=60)
                         GCATCGCCAATCGCCCAGATAATGTTGTTGGCGTATTCATTGAGGCCGTAGTTCACCGAACAGTCGGGCTGCGAACGAGTACCTTCATAATCCA                                                                     >  SRR4302543.365513/1‑94 (MQ=60)
                         GCATCGCCAATCGCCCAGATAATGTTGTTGGCGTATTCATTGAGGCCGTAGTTCACCGAACAGTCGGGCTGCGAACGAGTACCTTCATAATCCA                                                                     >  SRR4302543.7235353/1‑94 (MQ=60)
                          CATCGCCAATCGCCCAGATAATGTTGTTGGCGTATTCATTGAGGCCGTAGTTCACCGAACAGTCGGGCTGCGAACGAGTACCTTCATAATCCAC                                                                    <  SRR4302543.5798748/94‑1 (MQ=60)
                          CATCGCCAATCGCCCAGATAATGTTGTTGGCGTATTCATTGAGGCCGTAGTTCACCGAACAGTCGGGCTGCGAACGAGTACCTTCATAATCCAC                                                                    >  SRR4302543.6917672/1‑94 (MQ=60)
                           ATCGCCAATCGCCCAGATAATGTTGTTGGCGTATTCATTGAGGCCGTAGTTCACCGAACAGTCGGGCTGCGAACGAGTACCTTCATAATCCACG                                                                   >  SRR4302543.887782/1‑94 (MQ=60)
                             CGCCAATCGCCCAGATAATGTTGTTGGCGTATTCATTGAGGCCGTAGTTCACCGAACAGTCGGGCTGCGAACGAGTACCTTCATAATCCACGCC                                                                 <  SRR4302543.6555240/94‑1 (MQ=60)
                             CGCCAATCGCCCAGATAATGTTGTTGGCGTATTCATTGAGGCCGTAGTTCACCGAACAGTCGGGCTGCGAACGAGTACCTTCATAATCCACGCC                                                                 <  SRR4302543.3382018/94‑1 (MQ=60)
                             CGCCAATCGCCCAGATAATGTTGTTGGCGTATTCATTGAGGCCGTAGTTCACCGAACAGTCGGGCTGCGAACGAGTACCTTCATAATCCACGCC                                                                 >  SRR4302543.3323066/1‑94 (MQ=60)
                             CGCCAATCGCCCAGATAATGTTGTTGGCGTATTCATTGAGGCCGTAGTTCACCGAACAGTCGGGCTGCGAACGAGTACCTTCATAATCCACGCC                                                                 >  SRR4302543.6055452/1‑94 (MQ=60)
                               CCAATCGCCCAGATAATGTTGTTGGCGTATTCATTGAGGCCGTAGTTCACCGAACAGTCGGGCTGCGAACGAGTACCTTCATAATCCACGCCCA                                                               >  SRR4302543.7992149/1‑94 (MQ=60)
                               CCAATCGCCCAGATAATGTTGTTGGCGTATTCATTGAGGCCGTAGTTCACCGAACAGTCGGGCTGCGAACGAGTACCTTCATAATCCACGCCCA                                                               <  SRR4302543.3623519/94‑1 (MQ=60)
                               CCAATCGCCCAGATAATGTTGTTGGCGTATTCATTGAGGCCGTAGTTCACCGAACAGTCGGGCTGCGAACGAGTACCTTCATAATCCACGCCCA                                                               >  SRR4302543.4665924/1‑94 (MQ=60)
                               CCAATCGCCCAGATAATGTTGTTGGCGTATTCATTGAGGCCGTAGTTCACCGAACAGTCGGGCTGCGAACGAGTACCTTCATAATCCACGCCCA                                                               >  SRR4302543.7962672/1‑94 (MQ=60)
                               CCAATCGCCCAGATAATGTTGTTGGCGTATTCATTGAGGCCGTAGTTCACCGAACAGTCGGGCTGCGAACGAGTACCTTCATAATCCACGCCCA                                                               >  SRR4302543.5214984/1‑94 (MQ=60)
                                CAATCGCCCAGATAATGTTGTTGGCGTATTCATTGAGGCCGTAGTTCACCGAACAGTCGGGCTGCGAACGAGTACCTTCATAATCCACGCCCAG                                                              >  SRR4302543.829771/1‑94 (MQ=60)
                                CAATCGCCCAGATAATGTTGTTGGCGTATTCATTGAGGCCGTAGTTCACCGAACAGTCGGGCTGCGAACGAGTACCTTCATAATCCACGCCCAG                                                              >  SRR4302543.622256/1‑94 (MQ=60)
                                CAATCGCCCAGATAATGTTGTTGGCGTATTCATTGAGGCCGTAGTTCACCGAACAGTCGGGCTGCGAACGAGTACCTTCATAATCCACGCCCAG                                                              <  SRR4302543.4149644/94‑1 (MQ=60)
                                 AATCGCCCAGATAATGTTGTTGGCGTATTCATTGAGGCCGTAGTTCACCGAACAGTCGGGCTGCGAACGAGTACCTTCATAATCCACGCCCAGA                                                             <  SRR4302543.4561292/94‑1 (MQ=60)
                                  ATCGCCCAGATAATGTTGTTGGCGTATTCATTGAGGCCGTAGTTCACCGAACAGTCGGGCTGCGAACGAGTACCTTCATAATCCACGCCCAGAC                                                            <  SRR4302543.7630081/94‑1 (MQ=60)
                                  ATCGCCCAGATAATGTTGTTGGCGTATTCATTGAGGCCGTAGTTCACCGAACAGTCGGGCTGCGAACGAGTACCTTCATAATCCACGCCCAGAC                                                            <  SRR4302543.1571486/94‑1 (MQ=60)
                                   TCGCCCAGATAATGTTGTTCGCGTATTCATTGAGGCCGTAGTTCACCGAACAGTCGGGCTGCGAACGAGTACCTTCATAATCCACGCCCAGACC                                                           <  SRR4302543.7770756/94‑1 (MQ=60)
                                    CGCCCAGATAATGTTGTTGGCGTATTCATTGAGGCCGTAGTTCACCGAACAGTCGGGCTGCGAACGAGTACCTTCATAATCCACGCCCAGACCG                                                          >  SRR4302543.4707802/1‑94 (MQ=60)
                                    CGCCCAGATAATGTTGTTGGCGTATTCATTGAGGCCGTAGTTCACCGAACAGTCGGGCTGCGAACGAGTACCTTCATAATCCACGCCCAGACCG                                                          <  SRR4302543.6194059/94‑1 (MQ=60)
                                    CGCCCAGATAATGTTGTTGGCGTATTCATTGAGGCCGTAGTTCACCGAACAGTCGGGCTGCGAACGAGTACCTTCATAATCCACGCCCAGACCG                                                          >  SRR4302543.1080610/1‑94 (MQ=60)
                                    CGCCCAGATAATGTTGTTGGCGTATTCATCGAGGCCGTAGTTCACCGAACAGTCGGGCTGCGAACGAGTACCTTCATAATCCACGCCCAGACCG                                                          >  SRR4302543.483331/1‑94 (MQ=60)
                                      CCCAGATAATGTTGTTGGCGTATTCATTGAGGCCGTAGTTCACCGAACAGTCGGGCTGCGAACGAGTACCTTCATAATCCACGCCCAGACCGCC                                                        <  SRR4302543.926625/94‑1 (MQ=60)
                                       CCAGATAATGTTGTTGGCGTATTCATTGAGGCCGTAGTTCACCGAACAGTCGGGCTGCGAACGAGTACCTTCATAATCCACGCCCAGACCGCCG                                                       >  SRR4302543.3383102/1‑94 (MQ=60)
                                       CCAGATAATGTTGTTGGCGTATTCATTGAGGCCGTAGTTCACCGAACAGTCGGGCTGCGAACGAGTACCTTCATAATCCACGCCCAGACCGCCG                                                       <  SRR4302543.5184819/94‑1 (MQ=60)
                                       CCAGATAATGTTGTTGGCGTATTCATTGAGGCCGTAGTTCACCGAACAGTCGGGCTGCGAACCAGTACCTTCATAATCCACGCCCAGACCGCCG                                                       >  SRR4302543.5668109/1‑94 (MQ=60)
                                        CAGATAATGTTGTTGGCGTATTCATTGAGGCCGTAGTTCACCGAACAGTCGGGCTGCGAACGAGTACCTTCATAATCCACGCCCAGACCGCCGC                                                      >  SRR4302543.3230316/1‑94 (MQ=60)
                                         AGATAATGTTGTTGGCGTATTCATTGAGGCCGTAGTTCACCGAACAGTCGGGCTGCGAACGAGTACCTTCATAATCCACGCCCAGACCGCCGCC                                                     >  SRR4302543.2135005/1‑94 (MQ=60)
                                         AGATAATGTTGTTGGCGTATTCATTGAGGCCGTAGTTCACCGAACAGTCGGGCTGCGAACGAGTACCTTCATAATCCACGCCCAGACCGCCGCC                                                     <  SRR4302543.2180508/94‑1 (MQ=60)
                                          GATAATGTTGTTGGCGTATTCATTGAGGCCGTAGTTCACCGAACAGTCGGGCTGCGAACGAGTACCTTCATAATCCACGCCCAGACCGCCGCCG                                                    >  SRR4302543.4258915/1‑94 (MQ=60)
                                           ATAATGTTGTTGGCGTATTCATTGAGGCCGTAGTTCACCGAACAGTCGGGCTGCGAACGAGTACCTTCATAATCCACGCCCAGACCGCCGCCGC                                                   >  SRR4302543.3675143/1‑94 (MQ=60)
                                            TAATGTTGTTGGCGTATTCATTGAGGCCGTAGTTCACCGAACAGTCGGGCTGCGAACGAGTACCTTCATAATCCACGCCCAGACCGCCGCCGAC                                                  >  SRR4302543.6822926/1‑94 (MQ=60)
                                            TAATGTTGTTGGCGTATTCATTGAGGCCGTAGTTCACCGAACAGTCGGGCTGCGAACGAGTACCTTCATAATCCACGCCCAGACCGCCGCCGAC                                                  <  SRR4302543.1228323/94‑1 (MQ=60)
                                            TAATGTTGTTGGCGTATTCATTGAGGCCGTAGTTCACCGAACAGTCGGGCTGCGAACGAGTACCTTCATAATCCACGCCCAGACCGCCGCCGAC                                                  >  SRR4302543.5019620/1‑94 (MQ=60)
                                             AATGTTGTTGGCGTATTCATTGAGGCCGTAGTTCACCGAACAGTCGGGCTGCGAACGAGTACCTTCATAATCCACGCCCAGACCGCCGCCGACG                                                 >  SRR4302543.875876/1‑94 (MQ=60)
                                              ATGTTGTTGGCGTATTCATTGAGGCCGTAGTTCACCGAACAGTCGGGCTGCGAACGAGTACCTTCATAATCCACGCCCAGACCGCCGCCGACGT                                                >  SRR4302543.124823/1‑94 (MQ=60)
                                              ATGTTGTTGGCGTATTCATTGAGGCCGTAGTTCACCGAACAGTCGGGCTGCGAACGAGTACCTTCATAATCCACGCCCAGACCGCCGCCGACGT                                                >  SRR4302543.1097300/1‑94 (MQ=60)
                                              ATGTTGTTGGCGTATTCATTGAGGCCGTAGTTCACCGAACAGTCGGGCTGCGAACGAGTACCTTCATAATCCACGCCCAGACCGCCGCCGACGT                                                >  SRR4302543.2984127/1‑94 (MQ=60)
                                               TGTTGTTGGCGTATTCATTGAGGCCGTAGTTCACCGAACAGTCGGGCTGCGAACGAGTACCTTCATAATCCACGCCCAGACCGCCGCCGACGTC                                               >  SRR4302543.508482/1‑94 (MQ=60)
                                               TGTTGTTGGCGTATTCATTGAGGCCGTAGTTCACCGAACAGTCGGGCTGCGAACGAGTACCTTCATAATCCACGCCCAGACCGCCGCCGACGTC                                               <  SRR4302543.5308211/94‑1 (MQ=60)
                                                  TGTTGGCGTATTCATTGAGGCCGTAGTTCACCGAACAGTCGGGCTGCGAACGAGTACCTTCATAATCCACGCCCAGACCGCCGCCGACGTCGAA                                            >  SRR4302543.5494633/1‑94 (MQ=60)
                                                  TGTTGGCGTATTCATTGAGGCCGTAGTTCACCGAACAGTCGGGCTGCGAACGAGTACCTTCATAATCCACGCCCAGACCGCCGCCGACGTCGAA                                            <  SRR4302543.884194/94‑1 (MQ=60)
                                                   GTTGGCGTATTCATTGAGGCCGTAGTTCACCGAACAGTCGGGCTGCGAACGAGTACCTTCATAATCCACGCCCAGACCGCCGCCGACGTCGAAG                                           <  SRR4302543.1434434/94‑1 (MQ=60)
                                                   GTTGGCGTATTCATTGAGGCCGTAGTTCACCGAACAGTCGGGCTGCGAACGAGTACCTTCATAATCCACGCCCAGACCGCCGCCGACGCCGAAG                                           >  SRR4302543.298103/1‑94 (MQ=60)
                                                    TTGGCGTATTCATTGAGGCCGTAGTTCACCGAACAGTCGGGCTGCGAACGAGTACCTTCATAATCCACGCCCAGACCGCCGCCGACGTCGAAGC                                          >  SRR4302543.886489/1‑94 (MQ=60)
                                                    TTGGCGTATTCATTGAGGCCGTAGTTCACCGAACAGTCGGGCTGCGAACGAGTACCTTCATAATCCACGCCCAGACCGCCGCCGACGTCGAAGC                                          >  SRR4302543.1836469/1‑94 (MQ=60)
                                                    TTGGCGTATTCATTGAGGCCGTAGTTCACCGAACAGTCGGGCTGCGAACGAGTACCTTCATAATCCACGCCCAGACCGCCGCCGACGTCGAAGC                                          <  SRR4302543.890395/94‑1 (MQ=60)
                                                     TGGCGTATTCATTGAGGCCGTAGTTCACCGAACAGTCGGGCTGCGAACGAGTACCTTCATAATCCACGCCCAGACCGCCGCCGACGTCGAAGCC                                         >  SRR4302543.3336295/1‑94 (MQ=60)
                                                      GGCGTATTCATTGAGTCCGGAGGTCACCGAACAGTCGGACTGCGAACGAGTACCTTCATAATCCACGCCCAGACCGCCGCCGACGTCGAAGCAC                                        <  SRR4302543.4236845/94‑1 (MQ=60)
                                                      GGCGTATTCATTGAGGCCGTAGTTCACCGAACAGTCGGGCTGCGAACGAGTACCTTCATAATCCACGCCCAGACCGCCGCCGACGTCGAAGCAC                                        >  SRR4302543.2978445/1‑94 (MQ=60)
                                                      GGCGTATTCATTGAGGCCGTAGTTCACCGAACAGTCGGGCTGCGAACGAGTACCTTCATAATCCACGCCCAGACCGCCGCCGACGTCGAAGCAC                                        >  SRR4302543.4366483/1‑94 (MQ=60)
                                                        CGTATTCATTGAGGCCGTAGTTCACCGAACAGTCGGGCTGCGAACGAGTACCTTCATAATCCACGCCCAGACCGCCGCCGACGTCGAAGCACTG                                      >  SRR4302543.1952693/1‑94 (MQ=60)
                                                         GTATTCATTGAGGCCGTAGTTCACCGAACAGTCGGGCTGCGAACGAGTACCTTCATAATCCACGCCCAGACCGCCGCCGACGTCGAAGCACTGA                                     >  SRR4302543.6654727/1‑94 (MQ=60)
                                                         GTATTCATTGAGGCCGTAGTTCACCGAACAGTCGGGCTGCGAACGAGTACCTTCATAATCCACGCCCAGACCGCCGCCGACGTCGAAGCACTGA                                     >  SRR4302543.2955699/1‑94 (MQ=60)
                                                         GTATTCATTGAGGCCGTAGTTCACCGAACAGTCGGGCTGCGAACGAGTACCTTCATAATCCACGCCCAGACCGCCGCCGACGTCGAAGCACTGA                                     >  SRR4302543.2762542/1‑94 (MQ=60)
                                                         GTATTCATTGAGGCCGTAGTTCACCAAACAGTCGGGCTGCGAACGAGTACCTTCATAATCCACGCCCAGACCGCCGCCGACGTCGAAGCACTGA                                     >  SRR4302543.1419804/1‑94 (MQ=60)
                                                          TATTCATTGAGGCCGTAGTTCACCGAACAGTCGGGCTGCGAACGAGTACCTTCATAATCCACGCCCAGACCGCCGCCGACGTCGAAGCACTGAA                                    >  SRR4302543.6036066/1‑94 (MQ=60)
                                                           ATTCATTGAGGCCGTAGTTCACCGAACAGTCGGGCTGCGAACGAGTACCTTCATAATCCACGCCCAGACCGCCGCCGACGTCGAAGCACTGAAT                                   >  SRR4302543.238288/1‑94 (MQ=60)
                                                           ATTCATTGAGGCCGTAGTTCACCGAACAGTCGGGCTGCGAACGAGTACCTTCATAATCCACGCCCAGACCGCCGCCGACGTCGAAGCACTGAAT                                   <  SRR4302543.1415923/94‑1 (MQ=60)
                                                           ATTCATTGAGGCCGTAGTTCACCGAACAGTCGGGCTGCGAACGAGTACCTTCATAATCCACGCCCAGACCGCCGCCGACGTCGAAGCACTGAAT                                   <  SRR4302543.7912897/94‑1 (MQ=60)
                                                           ATTCATTGAGGCCGTAGTTCACCGAACAGTCGGGCTGCGAACGAGTACCTTCATAATCCACGCCCAGACCGCCGCCGACGTCGAAGCACTGAAT                                   >  SRR4302543.7796508/1‑94 (MQ=60)
                                                           ATTCATTGAGGCCGTAGTTCACCGAACAGTCGGGCTGCGAACGAGTACCTTCATAATCCACGCCCAGACCGCCGCCGACGTCGAAGCACTGAAT                                   <  SRR4302543.3345929/94‑1 (MQ=60)
                                                           ATTCATTGAGGCCGTAGTTCACCGAACAGTCGGGCTGCGAACGAGTACCTTCATAATCCACGCCCAGACCGCCGCCGACGTCGAAGCACTGAAT                                   <  SRR4302543.5282954/94‑1 (MQ=60)
                                                           ATTCATTGAGGCCGTAGTTCACCGAACAGTCGGGCTGCGAACGAGGACCTTCATAATCCACGCCCAGACCGCCGCCGACGTCGAAGCACTGAAT                                   >  SRR4302543.1972574/1‑94 (MQ=60)
                                                            TTCATTGAGGCCGTAGTTCACCGAACAGTCGGGCTGCGAACGAGTACCTTCATAATCCACGCCCAGACCGCCGCCGACGTCGAAGCACTGAATA                                  <  SRR4302543.5896847/94‑1 (MQ=60)
                                                            TTCATTGAGGCCGTAGTTCACCGAACAGTCGGGCTGCGAACGAGTACCTTCATAATCCACGCCCAGACCGCCGCCGACGTCGAAGCACTGAATA                                  <  SRR4302543.7850655/94‑1 (MQ=60)
                                                             TCATTGAGGCCGTAGTTCACCGAACAGTCGGGCTGCGAACGAGTACCTTCATAATCCACGCCCAGACCGCCGCCGACGTCGAAGCACTGAATAT                                 >  SRR4302543.3125620/1‑94 (MQ=60)
                                                             TCATTGAGGCCGTAGTTCACCGAACAGTCGGGCTGCGAACGAGTACCTTCATAATCCACGCCCAGACCGCCGCCGACGTCGAAGCACTGAATAT                                 <  SRR4302543.1563465/94‑1 (MQ=60)
                                                              CATTGAGGCCGTAGTTCACCGAACAGTCGGGCTGCGAACGAGTACCTTCATAATCCACGCCCAGACCGCCGCCGACGTCGAAGCACTGAATATT                                >  SRR4302543.5845394/1‑94 (MQ=60)
                                                              CATTGAGGCCGTAGTTCACCGAACAGTCGGGCTGCGAACGAGTACCTTCATAATCCACGCCCAGACCGCCGCCGACGTCGAAGCACTGAATATT                                >  SRR4302543.1038299/1‑94 (MQ=60)
                                                              CATTGAGGCCGTAGTTCACCGAACAGTCGGGCTGCGAACGAGTACCTTCATAATCCACGCCCAGACCGCCGCCGACGTCGAACCACTGATTATT                                >  SRR4302543.5321046/1‑94 (MQ=60)
                                                               ATTGAGGCCGTAGTTCACCGAACAGTCGGGCTGCGAACGAGTACCTTCATAATCCACGCCCAGACCGCCGCCGACGTCGAAGCACTGAATATTG                               >  SRR4302543.123805/1‑94 (MQ=60)
                                                               ATTGAGGCCGTAGTTCACCGAACAGTCGGGCTGCGAACGAGTACCTTCATAATCCACGCCCAGACCGCCGCCGACGTCGAAGCACTGAATATTG                               >  SRR4302543.1397068/1‑94 (MQ=60)
                                                               ATTGAGGCCGTAGTTCACCGAACAGTCGGGCTGCGAACGAGTACCTTCATAATCCACGCCCAGACCGCCGCCGACGTCGAAGCACTGAATATTG                               >  SRR4302543.7832560/1‑94 (MQ=60)
                                                               ATTGAGGCCGTAGTTCACCGAACAGTCGGGCTGCGAACGAGTACCTTCATAATCCACGCCCAGACCGCCGCCGACGTCGAAGCACTGAATATTG                               >  SRR4302543.3039692/1‑94 (MQ=60)
                                                               ATTGAGGCCGTAGTTCACCGAACAGTCGGGCTGCGAACGAGGACCTTCATCATCCACGCCCAGATCGCCGCCGACGTCGAAGCACTGtagatgg                               >  SRR4302543.3841217/1‑87 (MQ=60)
                                                                TTGAGGCCGTAGTTCACCGAACAGTCGGGCTGCGAACGAGTACCTTCATAATCCACGCCCAGACCGCCGCCGACGTCGAAGCACTGAATATTGA                              >  SRR4302543.1862816/1‑94 (MQ=60)
                                                                 TGAGGCCGTAGTTCACCGAACAGTCGGGCTGCGAACGAGTACCTTCATAATCCACGCCCAGACCGCCGCCGACGTCGAAGCACTGAATATTGAC                             <  SRR4302543.7900349/94‑1 (MQ=60)
                                                                 TGAGGCCGTAGTTCACCGAACAGTCGGGCTGCGAACGAGTACCTTCATAATCCACGCCCAGACCGCCGCCGACGTCGAAGCACTGAATATTGAC                             <  SRR4302543.6213510/94‑1 (MQ=60)
                                                                 TGAGGCCGTAGTTCACCGAACAGTCGGGCTGCGAACGAGTACCTTCATAATCCACGCCCAGACCGCCGCCGACGTCGAAGCACTGAATATTGAC                             <  SRR4302543.3564065/94‑1 (MQ=60)
                                                                 TGAGGCCGTAGTTCACCGAACAGTCGGGCTGCGAACGAGTACCTTCATAATCCACGCCCAGACCGCCGCCGACGTCGAAGCACTGAATATTGAC                             <  SRR4302543.4220041/94‑1 (MQ=60)
                                                                 TGAGGCCGTAGTTCACCGAACAGTCGGGCTGCGAACGAGTACCTTCATAATCCACGCCCAGACCGCCGCCGACGTCGAAGCACTGAATATTGAC                             <  SRR4302543.286902/94‑1 (MQ=60)
                                                                 TGAGGCCGTAGTTCACCGAACAGTCGGGCTGCGAACGAGTACCTTCATAATCCACGCCCAGACCGCCGCCGACGTCGAAGCACTGAATATTGAC                             <  SRR4302543.7375727/94‑1 (MQ=60)
                                                                 TGAGGCCGTAGTTCACCGAACAGTCGGGCTGCGAACGAGTACCTTCATAATACACGCCCAGACCGCCGCCGACGTCGAAGCACTGAATATTGAC                             <  SRR4302543.5300331/94‑1 (MQ=60)
                                                                 TGAGGCCGTAGTTCACCGAACAGTCGGGCTGCGAACGAGTACCTTCATAATACACGCCCAGACCGCCGCCGACGTCGAAGCACTGAATATTGAC                             <  SRR4302543.5300330/94‑1 (MQ=60)
                                                                   AGGCCGTAGTTCACCGAACAGTCGGGCTGCGAACGAGTACCTTCATAATCCACGCCCAGACCGCCGACGACGTCGAAGCACTGAATATTGACGC                           >  SRR4302543.3762816/1‑94 (MQ=60)
                                                                    GGCCGTAGTTCACCGAACAGTCGGGCTGCGAACTAGTACCTTCATAATCCACGCCCAGACCGCCGCCGACGTCGAAGCACTGAATATTGACGCC                          <  SRR4302543.5877195/94‑1 (MQ=60)
                                                                     GCCGTAGTTCACCGAACAGTCGGGCTGCGAACGAGTACCTTCATAATCCACGCCCAGACCGCCGCCGACGTCGAAGCACTGAATATTGACGCCC                         <  SRR4302543.1516363/94‑1 (MQ=60)
                                                                      CCGTAGTTCACCGAACAGTCGGGCTGCGAACGAGTACCTTCATAATCCACGCCCAGACCGCCGCCGACGTCGAAGCACTGAATATTGACGCCCA                        <  SRR4302543.1792715/94‑1 (MQ=60)
                                                                       CGTAGTTCACCGAACAGTCGGGCTGCGAACGAGTACCTTCATAATCCACGCCCAGACCGCCGCCGACGTCGAAGCACTGAATATTGACGCCCAG                       <  SRR4302543.3414754/94‑1 (MQ=60)
                                                                       CGTAGTTCACCGAACAGTCGGGCTGCGAACGAGTACCTTCATAATCCACGCCCAGACCGCCGCCGACGTCGAAGCACTGAATATTGACGCCCAG                       >  SRR4302543.2859324/1‑94 (MQ=60)
                                                                        GTAGTTCACCGAACAGTCGGGCTGCGAACGAGTACCTTCATAATCCACGCCCAGACCGCCGCCGACGTCGAAGCACTGAATATTGACGCCCAGC                      <  SRR4302543.32387/94‑1 (MQ=60)
                                                                        GTAGTTCACCGAACAGTCGGGCTGCGAACGAGTACCTTCATAATCCACGCCCAGACCGCCGCCGACGTCGAAGCACTGAATATTGACGCCCAGC                      >  SRR4302543.2273638/1‑94 (MQ=60)
                                                                         TAGTTCACCGAACAGTCGGGCTGCGAACGAGTACCTTCATAATCCACGCCCAGACCGCCGCCGACGTCGAAGCACTGAATATTGACGCCCAGCT                     <  SRR4302543.2716932/94‑1 (MQ=60)
                                                                          AGTTCCCCGAACAGTCGGGCTGCAAACGAGTACCTTCATAACCCACGCCCAGCCCGCCGCCGACGTCGAAGCACTGAATATTGACGCCCAGCTT                    <  SRR4302543.6158523/94‑1 (MQ=60)
                                                                          AGTTCACCGAACAGTCGGGCTGCGAACGAGTACCTTCATAATCCACGCCCAGACCGCCGCCGACGTCGAAGCACTGAATATTGACGCCCAGCTT                    >  SRR4302543.4550451/1‑94 (MQ=60)
                                                                            TTCACCGAACAGTCGGGCTGCGAACGAGTACCTTCATAATCCACGCCCAGACCGCCGCCGACGTCGAAGCACTGAATATTGACGCCCAGCTTGT                  >  SRR4302543.2875297/1‑94 (MQ=60)
                                                                               ACCGAACAGTCGGGCTGCGAACGAGTACCTTCATAATCCACGCCCAGACCGCCGCCGACGTCGAAGCACTGAATATTGACGCCCAGCTTGTGCA               <  SRR4302543.1571180/94‑1 (MQ=60)
                                                                               ACCGAACAGTCGGGCTGCGAACGAGTACCTTCATAATCCACGCCCAGACCGCCGCCGACGTCGAAGCACTGAATATTGACGCCCAGCTTGTGCA               <  SRR4302543.6326332/94‑1 (MQ=60)
                                                                               ACCGAACAGACGGGCTGCGAACGAGTACCTTCATAATCCACGCCCAGACCGCCGCCGACGTCGCAGCACTGAATATTGACGCCCAGCTTGTGCA               >  SRR4302543.925176/1‑94 (MQ=60)
                                                                                CCGAACAGTCGGGCTGCGAACGAGTACCTTCATAATCCACGCCCAGACCGCCGCCGACGTCGAAGCACTGAATATTGACGCCCAGCTTGTGCAG              >  SRR4302543.6169307/1‑94 (MQ=60)
                                                                                CCGAACAGTCGGGCTGCGAACGAGTACCTTCATAATCCACGCCCAGACCGCCGCCGACGTCGAAGCACTGAATATTGACGCCCAGCTTGTGCAG              >  SRR4302543.3917401/1‑94 (MQ=60)
                                                                                CCGAACAGTCGGGCTGCGAACGAGTACCTTCATAATCCACGCCCAGACCGCCGCCGACGTCGAAGCACTGAATATTGACGCCCAGCTTGTGCAG              >  SRR4302543.6169306/1‑94 (MQ=60)
                                                                                CCGAACAGTCGGGCTGCGAACGAGTACCTTCATAATCCACGCCCAGACCGCCGCCGACGTCGAAGCACTGAATATTGACGCCCAGCTTGTGCAG              <  SRR4302543.7191920/94‑1 (MQ=60)
                                                                                CCGAACAGTCGGGCTGCGAACGAGTACCTTCATAATCCACGCCCAGACCGCCGCCGACGTCGAAGCACTGAATATTGACGCCCAGCTTGTGCAG              <  SRR4302543.781462/94‑1 (MQ=60)
                                                                                 CGAACAGTCGGGCTGGGAACGAGTACCTTCATAATCCACGCCCAGGCCGCCGCCGACGTCGAAGCACTGAATATTGACGCCCAGCTTGTGCAGT             <  SRR4302543.3857616/94‑1 (MQ=60)
                                                                                 CGAACAGTCGGGCTGCGAACGAGTACCTTCATAATCCACGCCCAGACCGCCGCCGACGTCGAAGCACTGAATATTGACGCCCAGCTTGTGCAGT             >  SRR4302543.7179520/1‑94 (MQ=60)
                                                                                    ACAGTCGGGCTGCGAACGAGTACCTTCATAATCCACGCCCAGACCGCCGCCGACGTCGAAGCACTGAATATTGACGCCCAGCTTGTGCAGTTCC          <  SRR4302543.7188309/94‑1 (MQ=60)
                                                                                    ACAGTCGGGCTGCGAACGAGTACCTTCATAATCCACGCCCAGACCGCCGCCGACGTCGAAGCACTGAATATTGACGCCCAGCTTGTGCAGTTCC          <  SRR4302543.4541161/94‑1 (MQ=60)
                                                                                      AGTCGGGCTGCGAACGAGTACCTTCATAATCCACGCCCAGACCGCCGCCGACGTCGAAGCACTGAATATTGACGCCCAGCTTGTGCAGTTCCAC        >  SRR4302543.6302769/1‑94 (MQ=60)
                                                                                      AGTCGGGCTGCGAACGAGTACCTTCATAATCCACGCCCAGACCGCCGCCGACGTCGAAGCACTGAATATTGACGCCCAGCTTGTGCAGTTCCAC        <  SRR4302543.5101451/94‑1 (MQ=60)
                                                                                       GTCGGGCTGCGAACGAGTACCTTCATAATCCACGCCCAGACCGCCGCCGACGTCGAAGCACTGAATATTGACGCCCAGCTTGTGCAGTTCCACA       <  SRR4302543.5610099/94‑1 (MQ=60)
                                                                                        TCGGGCTGCGAACGAGTACCTTCATAATCCACGCCCAGACCGCCGCCGACGTCGAAGCACTGAATATTGACGCCCAGCTTGTGCAGTTCCACAT      <  SRR4302543.1976926/94‑1 (MQ=60)
                                                                                            GCTGCGAACGAGTACCTTCATAATCCACGCCCAGACCGCCGCCGACGTCGAAGCACTGAATATTGACGCCCAGCTTGTGCAGTTCCACATAGAA  <  SRR4302543.1512626/94‑1 (MQ=60)
                                                                                            |                                                                                             
ATGCGGCAGACCGTTTTCTTCACACGCATCGCCAATCGCCCAGATAATGTTGTTGGCGTATTCATTGAGGCCGTAGTTCACCGAACAGTCGGACTGCGAACGAGTACCTTCATAATCCACGCCCAGACCGCCGCCGACGTCGAAGCACTGAATATTGACGCCCAGCTTGTGCAGTTCCACATAGAA  >  NC_000913/3082869‑3083054

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑