Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 1696 279.3 15874187 97.8% 15524954 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,083,359 T→C H191R (CAC→CGC)  speA ← biosynthetic arginine decarboxylase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,083,3590TC99.5% 1197.7 / ‑5.2 383H191R (CAC→CGC) speAbiosynthetic arginine decarboxylase
Reads supporting (aligned to +/- strand):  ref base T (0/2);  new base C (184/197);  total (184/199)
Fisher's exact test for biased strand distribution p-value = 4.99e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01

Only 100 of 391 total aligned reads displayed.

ACGCCCAGACGAGGAACGACATTCAGACGTTCTGCTTCATCCAGCACAATGGCGATTTCTGACATCTTCTCAATGACCAGATAGACCTTGTGCCCCATCTTCTCGCCAATTAATGCCAGGCGGATATATTCGCGGTCTTTATAACCGTTGCAGACGATGACGCTACGGGTCATGCCAGCA  >  NC_000913/3083269‑3083448
                                                                                          |                                                                                         
 cgcCCAGACGAGGAACGACATTCAGACGTTCTGCTTCATCCAGCACAATGGCGATTTCTGACATCTTCTCAATGACCAGATAGACCTTGCGcc                                                                                        >  1:1214221/1‑93 (MQ=255)
 cgcCCAGACGAGGAACGACATTCAGACGTTCTGCTTCATCCAGCACAATGGCGATTTCTGACATCTTCTCAATGACCAGATAGACCTTGCGcc                                                                                        <  1:648262/93‑1 (MQ=255)
 cgcCCAGACGAGGAACGACATTCAGACGTTCTGCTTCATCCAGCACAATGGCGATTTCTGACATCTTCTCAATGACCAGATAGACCTTGCGcc                                                                                        >  2:6529532/1‑93 (MQ=255)
   cccAGACGAGGAACGACATTCAGACGTTCTGCTTCATCCAGCACAATGGCGATTTCTGACATCTTCTCAATGACCAGATAGACCTTGCGcccc                                                                                      >  1:2870710/1‑93 (MQ=255)
   cccAGACGAGGAACGACATTCAGACGTTCTGCTTCATCCAGCACAATGGCGATTTCTGACATCTTCTCAATGACCAGATAGACCTCGCGcccc                                                                                      >  2:4118425/1‑93 (MQ=255)
    ccAGACGAGGAACGACATTCAGACGTTCTGCTTCATCCAGCACAATGGCGATTTCTGACATCTTCTCAATGACCAGATAGACCTTGCGCCCCa                                                                                     <  1:2732210/93‑1 (MQ=255)
    ccAGACGAGGAACGACATTCAGACGTTCTGCTTCATCCAGCACAATGGCGATTTCTGACATCTTCTCAATGACCAGATAGACCTTGCGCCCCa                                                                                     <  2:443572/93‑1 (MQ=255)
     cAGACGAGGAACGACATTCAGACGTTCTGCTTCATCCAGCACAATGGCGATTTCTGACATCTTCTCAATGACCAGATAGACCTTGCGCCCCAt                                                                                    <  2:2929092/93‑1 (MQ=255)
     cAGACGAGGAACGACATTCAGACGTTCTGCTTCATCCAGCACAATGGCGATTTCTGACATCTTCTCAATGACCAGATAGACCTTGCGCCCCAt                                                                                    <  2:3030264/93‑1 (MQ=255)
     cAGACGAGGAACGACATTCAGACGTTCTGCTTCATCCAGCACAATGGCGATTTCTGACATCTTCTCAATGACCAGATAGACCTTGCACCCCAt                                                                                    >  2:3434301/1‑93 (MQ=255)
       gACGAGGAACGACATTCAGACGTTCTGCTTCATCCAGCACAATGGCGATTTCTGACATCTTCTCAATGACCAGATAGACCTTGCGCCCCAtct                                                                                  <  2:5874367/93‑1 (MQ=255)
       gACGAGGAACGACATTCAGACGTTCTGCTTCATCCAGCACAATGGCGATTTCTGACATCTTCTCAATGACCAGATAGACCTTGCGCCCCAtct                                                                                  >  2:7235782/1‑93 (MQ=255)
        aCGAGGAACGACATTCAGACGTTCTGCTTCATCCAGCACAATGGCGATTTCTGACATCTTCTCAATGACCAGATAGACCTTGCGCCCCAtctt                                                                                 >  1:2307416/1‑93 (MQ=255)
          gAGGAACGACATTCAGACGTTCTGCTTCATCCAGCACAATGGCGATTTCTGACATCTTCTCAATGACCAGATAGACCTTGCGCCCCAtcttct                                                                               >  1:1574365/1‑93 (MQ=255)
          gAGGAACGACATTCAGACGTTCTGCTTCATCCAGCACAATGGCGATTTCTGACATCTTCTCAATGACCAGATAGACCTTGCGCCCCAtcttct                                                                               >  2:1783150/1‑93 (MQ=255)
          caGGAACGACATTCAGACGTTCTGCTTCATCCAGCACAATGGCGATTTCTGACATCTCCTCAATGACCAGATAGACCTTGCGCCCCAtcttct                                                                               <  1:2637632/92‑1 (MQ=255)
           aGGAACGACATTCAGACGTTCTGCTTCATCCAGCACAATGGCGATTTCTGACATCTTCTCAATGACCAGATAGACCTTGCGCCCCATCTtctc                                                                              <  1:6640379/93‑1 (MQ=255)
            ggAACGACATTCAGACGTTCTGCTTCATCCAGCACAATGGCGATTTCTGACATCTTCTCAATGACCAGATAGACCTTGCGCCCCATCTTCTCg                                                                             >  2:1083552/1‑93 (MQ=255)
            ggAACGACATTCAGACGTTCTGCTTCATCCAGCACAATGGCGATTTCTGACATCTTCTCAATGACCAGATAGACCTTGCGCCCCATCTTCTCg                                                                             >  1:4293944/1‑93 (MQ=255)
              aaCGACATTCAGACGTTCTGCTTCATCCAGCACAATGGCGATTTCTGACATCTTCTCAATGACCAGATAGACCTTGCGCCCCATCTTCTCGcc                                                                           >  1:1648300/1‑93 (MQ=255)
               aCGACATTCAGACGTTCTGCTTCATCCAGCACAATGGCGATTTCTGACATCTTCTCAATGACCAGATAGACCTTGCGCCCCATCTTCTCGCCa                                                                          >  1:426665/1‑93 (MQ=255)
                cGACATTCAGACGTTCTGCTTCATCCAGCACAATGGCGATTTCTGACATCTTCTCAATGACCAGATAGACCTTGCGCCCCATCTTCTCGCCaa                                                                         <  2:6225512/93‑1 (MQ=255)
                cGACATTCAGACGTTCTGCTTCATCCAGCACAATGGCGATTTCTGACATCTTCTCAATGACCAGATAGACCTTGCGCCCCATCTTCTCGCCaa                                                                         <  2:7739220/93‑1 (MQ=255)
                 gACATTCAGACGTTCTGCTTCATCCAGCACAATGGCGATTTCTGACATCTTCTCAATGACCAGATAGACCTTGCGCCCCATCTTCTCGACAAt                                                                        >  2:7671308/1‑93 (MQ=255)
                  aCATTCAGACGTTCTGCTTCATCCAGCACAATGGCGATTTCTGACATCTTCTCAATGACCAGATAGACCTTGCGCCCCATCTTCTCGCCAAtt                                                                       >  2:467763/1‑93 (MQ=255)
                    aTTCAGACGTTCTGCTTCATCCAGCACAATGGCGATTTCTGACATCTTCTCAATGACCAGATAGACCTTGCGCCCCATCTTCTCGCCAATTaa                                                                     <  1:7139207/93‑1 (MQ=255)
                    aTTCAGACGTTCTGCTTCATCCAGCACAATGGCGATTTCTGACATCTTCTCAATGACCAGATAGACCTTGCGCCCCATCTTCTCGCCAATTaa                                                                     <  2:5716380/93‑1 (MQ=255)
                    aTTCAGACGTTCTGCTTCATCCAGCACAATGGCGATTTCTGACATCTTCTCAATGACCAGATAGACCTTGCGCCCCATCTTCTCGCCAATTaa                                                                     >  2:6596131/1‑93 (MQ=255)
                    aTTCAGACGTTCTGCTTCATCCAGCACAATGGCGATTTCTGACATCTTCTCAATGACCAGATAGACCTTGCGCCCCATCTTCTCGCCAATTaa                                                                     >  2:7655051/1‑93 (MQ=255)
                     ttCAGACGTTCTGCTTCATCCAGCACAATGGCGATTTCTGACATCTTCTCAATGACCAGATAGACCTTGCGCCCCATCTTCTCGCCAATTAAt                                                                    <  1:7761977/93‑1 (MQ=255)
                     ttCAGACGTTCTGCTTCATCCAGCACAATGGCGATTTCTGACATCTTCTCAATGACCAGATAGACCTTGCGCCCCATCTTCTCGCCAATTAAt                                                                    >  2:2483653/1‑93 (MQ=255)
                     ttCAGACGTTCTGCTTCATCCAGCACAATGGCGATTTCTGACATCTTCTCAATGACCAGATAGACCTTGCGCCCCATCTTCTCGCCAATTAAt                                                                    >  2:1720954/1‑93 (MQ=255)
                     ttCAGACGTTCTGCTTCATCCAGCACAATGGCGATTTCTGACATCTTCTCAATGACCAGATAGACCTTGCGCCCCATCTTCTCGCCAATTAAt                                                                    >  2:3844757/1‑93 (MQ=255)
                        aGACGTTCTGCTTCATCCAGCACAATGGCGATTTCTGACATCTTCTCAATGACCAGATAGACCTTGCGCCCCATCTTCTCGCCAATTAATGcc                                                                 <  1:3451554/93‑1 (MQ=255)
                        aGACGTTCTGCTTCATCCAGCACAATGGCGATTTCTGACATCTTCTCAATGACCAGATAGACCTTGCGCCCCATCTTCTCGCCAATTAATGcc                                                                 <  2:5992609/93‑1 (MQ=255)
                         gACGTTCTGCTTCATCCAGCACAATGGCGATTTCTGACATCTTCTCAATGACCAGATAGACCTTGCGCCCCATCTTCTCGCCAATTAATGCCa                                                                >  1:5581807/1‑93 (MQ=255)
                          aCGTTCTGCTTCATCCAGCACAATGGCGATTTCTGACATCTTCTCAATGACCAGATAGACCTTGCGCCCCATCTTCTCGCCAATTAATGCCAg                                                               >  1:127856/1‑93 (MQ=255)
                            gTTCTGCTTCATCCAGCACAATGGCGATTTCTGACATCTTCTCAATGACCAGATAGACCTTGCGCCCCATCTTCTCGCCAATTAATGCCAGGc                                                             >  2:2818676/1‑93 (MQ=255)
                               cTGCTTCATCCAGCACAATGGCGATTTCTGACATCTTCTCAATGACCAGATAGACCTTGCGCCCCATCTTCTCGCCAATTAATGCCAGGCGGa                                                          <  1:3011847/93‑1 (MQ=255)
                               cTGCTTCATCCAGCACAATGGCGATTTCTGACATCTTCTCAATGACCAGATAGACCTTGCGCCCCATCTTCTCGCCAATTAATGCCAGGCGGa                                                          <  2:877370/93‑1 (MQ=255)
                                tGCTTCATCCAGCACAATGGCGATTTCTGACATCTTCTCAATGACCAGATAGACCTTGCGCCCCATCTTCTCGCCAATTAATGCCAGGCGGat                                                         <  1:1081756/93‑1 (MQ=255)
                                  cTTCATCCAGCACAATGGCGATTTCTGACATCTTCTCAATGACCAGATAGACCTTGCGCCCCATCTTCTCGCCAATTAATGCCAGGCGGatat                                                       <  1:6872099/93‑1 (MQ=255)
                                    tCATCCAGCACAATGGCGATTTCTGACATCTTCTCAATGACCAGATAGACCTTGCGCCCCATCTTCTCGCCAATTAATGCCAGGCGGatatat                                                     >  1:7194823/1‑93 (MQ=255)
                                    tCATCCAGCACAATGGCGATTTCTGACATCTTCTCAATGACCAGATAGACCTTGCGCCCCATCTTCTCGCCAATTAATGCCAGGCGGatatat                                                     <  1:320957/93‑1 (MQ=255)
                                     cATCCAGCACAATGGCGATTTCTGACATCTTCTCAATGACCAGATAGACCTTGCGCCCCATCTTCTCGCCAATTAATGCCAGGCGGATATAtt                                                    <  2:6987212/93‑1 (MQ=255)
                                     cATCCAGCACAATGGCGATTTCTGACATCTTCTCAATGACCAGATAGACCTTGCGCCCCATCTTCTCGCCAATTAATGCCAGGCGGATATAtt                                                    <  1:4098342/93‑1 (MQ=255)
                                      aTCCAGCACAATGGCGATTTCTGACATCTTCTCAATGACCAGATAGACCTTGCGCCCCATCTTCTCGCCAATTAATGCCAGGCGGATATATTc                                                   <  1:5479519/93‑1 (MQ=255)
                                         cAGCACAATGGCGATTTCTGACATCTTCTCAATGACCAGATAGACCTTGCGCCCCATCTTCTCGCCAATTAATGCCAGGCGGATATATTcgcg                                                >  1:2006765/1‑93 (MQ=255)
                                         cAGCACAATGGCGATTTCTGACATCTTCTCAATGACCAGATAGACCTTGCGCCCCATCTTCTCGCCAATTAATGCCAGGCGGATATATTcgcg                                                >  1:4934596/1‑93 (MQ=255)
                                          aGCACAATGGCGATTTCTGACATCTTCTCAATGACCAGATAGACCTTGCGCCCCATCTTCTCGCCAATTAATGCCAGGCGGATATATTCGCgg                                               <  2:6414215/93‑1 (MQ=255)
                                           gCACAATGGCGATTTCTGACATCTTCTCAATGACCAGATAGACCTTGCGCCCCATCTTCTCGCCAATTAATGCCAGGCGGATATATTCGCGGt                                              >  1:6794579/1‑93 (MQ=255)
                                           gCACAATGGCGATTTCTGACATCTTCTCAATGACCAGATAGACCTTGCGCCCCATCTTCTCGCCAATTAATGCCAGGCGGATATATTCGCGGt                                              <  2:672358/93‑1 (MQ=255)
                                             acaATGGCGATTTCTGACATCTTCTCAATGACCAGATAGACCTTGCGCCCCATCTTCTCGCCAATTAATGCCAGGCGGATATATTCGCGGTCt                                            >  1:1418071/1‑93 (MQ=255)
                                             acaATGGCGATTTCTGACATCTTCTCAATGACCAGATAGACCTTGCGCCCCATCTTCTCGCCAATTAATGCCAGGCGGATATATTCGCGGTCt                                            <  2:7401953/93‑1 (MQ=255)
                                             acaATGGCGATTTCTGACATCTTCTCAATGACCAGATAGACCTTGCGCCCCATCTTCTCGCCAATTAATGCCAGGCGGATATATTCGCGGTCt                                            >  1:3808428/1‑93 (MQ=255)
                                              caATGGCGATTTCTGACATCTTCTCAATGACCAGATAGACCTTGCGCCCCATCTTCTCGCCAATTAATGCCAGGCGGATATATTCGCGGTCtt                                           <  2:6473231/93‑1 (MQ=255)
                                              caATGGCGATTTCTGACATCTTCTCAATGACCAGATAGACCTTGCGCCCCATCTTCTCGCCAATTAATGCCAGGCGGATATATTCGCGGCCtt                                           <  2:2160332/93‑1 (MQ=255)
                                               aaTGGCGATTTCTGACATCTTCTCAATGACCAGATAGACCTTGCGCCCCATCTTCTCGCCAATTAATGCCAGGCGGATATATTCGCGGTCttt                                          >  2:1667925/1‑93 (MQ=255)
                                               aaTGGCGATTTCTGACATCTTCTCAATGACCAGATAGACCTTGCGCCCCATCTTCTCGCCAATTAATGCCAGGCGGATATATTCGCGGTCttt                                          <  1:7267935/93‑1 (MQ=255)
                                                aTGGCGATTTCTGACATCTTCTCAATGACCAGATAGACCTTGCGCCCCATCTTCTCGCCAATTAATGCCAGGCGGATATATTCGCGGTCTTta                                         >  2:7075758/1‑93 (MQ=255)
                                                   gCGATTTCTGACATCTTCTCAATGACCAGATAGACCTTGCGCCCCATCTTCTCGCCAATTAATGCCAGGCGGATATATTCGCGGTCTTTATaa                                      <  1:176258/93‑1 (MQ=255)
                                                    gcaTTTCTGACATCTTCTCAATGACCAGATAGACCTTGCGCCCCATCTTCTCGCCAATTAATGCCAGGCGGATATATTCGCGGTCTTTATAAc                                     <  1:2063/91‑1 (MQ=255)
                                                    cGATTTCTGACATCTTCTCAATGACCAGATAGACCTTGCGCCCCATCTTCTCGCCAATTAATGCCAGGCGGATATATTCGCGGTCTTTATAAc                                     <  1:6152003/93‑1 (MQ=255)
                                                    cGATTTCTGACATCTTCTCAATGACCAGATAGACCTTGCGCCCCATCTTCTCGCCAATTAATGCCAGGCGGATATATTCGCGGTCTTTATAAc                                     <  2:7839723/93‑1 (MQ=255)
                                                    cGATTTCTGACATCTTCTCAATGACCAGATAGACCTTGCGCCCCATCTTCTCGCCAATTAATGCCAGGCGGATATATTCGCGGTCTTTATAAc                                     <  1:6574171/93‑1 (MQ=255)
                                                    cGATTTCTGACATCTTCTCAATGACCAGATAGACCTTGCGCCCCATCTTCTCGCCAATTAATGCCAGGCGGATATATTCGCGGTCTTTATAAc                                     <  1:4998464/93‑1 (MQ=255)
                                                     gATTTCTGACATCTTCTCAATGACCAGATAGACCTTGCGCCCCATCTTCTCGCCAATTAATGCCAGGCGGATATATTCGCGGTCTTTATAAcc                                    <  2:4917700/93‑1 (MQ=255)
                                                      aTTTCTGACATCTTCTCAATGACCAGATAGACCTTGCGCCCCATCTTCTCGCCAATTAATGCCAGGCGGATATATTCGCGGTCTTTATAACCg                                   >  1:2225912/1‑93 (MQ=255)
                                                       tttCTGACATCTTCTCAATGACCAGATAGACCTTGCGCCCCATCTTCTCGCGAATTAATGCCAGCCGGATATATTCGCGGTCTTTATAACCGt                                  >  2:5166938/1‑93 (MQ=255)
                                                       tttCTGACATCTTCTCAATGACCAGATAGACCTTGCGCCCCATCTTCTCGCCAATTAATGCCAGGCGGATATATTCGCGGTCTTTATAACCGt                                  <  1:3619786/93‑1 (MQ=255)
                                                       tttCTGACATCTTCTCAATGACCAGATAGACCTTACGCCCCATCTTCTCGCCAATTAATGCCAGGCGGATATATTCGCGGTCTTTATAACCGt                                  >  2:1159667/1‑93 (MQ=255)
                                                            gACATCTTCTCAATGACCAGATAGACCTTGCGCCCCATCTTCTCGCCAATTAATGCCAGGCGGATATATTCGCGGTCTTTATAACCGTTGCAg                             >  2:2301564/1‑93 (MQ=255)
                                                             aCATCTTCTCAATGACCAGATAGACCTTGCGCCCCATCTTCTCGCCAATTAATGCCAGGCGGATATATTCGCGGTCTTTATAACCGTTGCAGa                            <  1:3715518/93‑1 (MQ=255)
                                                             aCATCTTCTCAATGACCAGATAGACCTTGCGCCCCATCTTCTCGCCAATTAATGCCAGGCGGATATATTCGCGGTCTTTATAACCGTTGCAGa                            >  1:3359475/1‑93 (MQ=255)
                                                                tcttctCAATGACCAGATAGACCTTGCGCCCCATCTTCTCGCCAATTAATGCCAGGCGGATATATTCGCGGTCTTTATAACCGTTGCAGACGa                         <  2:3623311/93‑1 (MQ=255)
                                                                tcttctCAATGACCAGATAGACCTTGCGCCCCATCTTCTCGCCAATTAATGCCAGGCGGATATATTCGCGGTCTTTATAACCGTTGCAGACGa                         >  2:3526672/1‑93 (MQ=255)
                                                                 cttctCAATGCCCAGATAGACCTTGCGCCCCATCTTCTCTCCAATTAATGCCAGGCGGATATATTCGCGGTCTTTATAACCGTTGCAAACGAt                        <  2:4468609/93‑1 (MQ=255)
                                                                  ttctCAATGACCAGATAGACCTTGCGCCCCATCTTCTCGCCAATTAATGCCAGGCGGATATATTCGCGGTCTTTATAACCGTTGCAGACGATg                       <  1:797670/93‑1 (MQ=255)
                                                                   tctcAATGACCAGATAGACCTTGCGCCCCATCTTCTCGCCAATTAATGCCAGGCGGATATATTCGCGGTCTTTATAACCGTTGCAGACGATGa                      <  2:2742397/93‑1 (MQ=255)
                                                                      cAATGACCAGATAGACCTTGCGCCCCATCTTCTCGCCAATTAATGCCAGGCGGATATATTCGCGGTCTTTATAACCGTTGCAGACGATGACGc                   >  1:1028147/1‑93 (MQ=255)
                                                                        aTGACCAGATAGACCTTGCGCCCCATCTTCTCGCCAATTAATGCCAGGCGGATATATTCGCGGTCTTTATAACCGTTGCAGACGATGACGCTa                 <  1:1932034/93‑1 (MQ=255)
                                                                        aTGACCAGATAGACCTTGCGCCCCATCTTCTCGCCAATTAATGCCAGGCGGATATATTCGCGGTCTTTATAACCGTTGCAGACGATGACGCTa                 <  1:520101/93‑1 (MQ=255)
                                                                         tGACCAGATAGACCTTGCGCCCCATCTTCTCGCCAATTAATGCCAGGCGGATATATTCGCGGTCTTTATAACCGTTGCAGACGATGACGCTAc                >  2:131146/1‑93 (MQ=255)
                                                                          gACCAGATAGACCTTGCGCCCCATCTTCTCGCCAATTAATGCCAGGCGGATATATTCGCGGTCTTTATAACCGTTGCAGACGATGACGCTACg               >  2:1511588/1‑93 (MQ=255)
                                                                          gACCAGATAGACCTTGCGCCCCATCTTCTCGCCAATTAATGCCAGGCGGATATATTCGCGGTCTTTATAACCGTTGCAGACGATGACGCTACg               >  1:3276297/1‑93 (MQ=255)
                                                                          gACCAGATAGACCTTGCGCCCCATCTTCTCGCCAATTAATGCCAGGCGGATATATTCGCGGTCTTTATAACCGTTGCAGACGATGACGCTACg               <  1:4589960/93‑1 (MQ=255)
                                                                           aCCAGATAGACCTTGCGCCCCATCTTCTCGCCAATTAATGCCAGGCGGATATATTCGCGGTCTTTATAACCGTTGCAGACGATGACGCTACgg              >  2:4195838/1‑93 (MQ=255)
                                                                              aGATAGACCTTGCGCCCCATCTTCTCGCCAATTAATGCCAGGCGGATATATTCGCGGTCTTTATAACCGTTGCAGACGATGACGCTACGGGTc           <  1:7072036/93‑1 (MQ=255)
                                                                               taTAGACCTTGCGCCCCATCTTCTCGCCAATTAATGCCAGGCGGATATATTCGCGGTCTTTATAACCGTTGCAGACGATGACGCTACGGGTCa          <  1:5396264/92‑1 (MQ=255)
                                                                               gATAGACCTTGCGCCCCATCTTCTCGCCAATTAATGCCAGGCGGATATATTCGCGGTCTTTATAACCGTTGCAGACGATGACGCTACGGGTCa          >  2:3324075/1‑93 (MQ=255)
                                                                               gATAGACCTTGCGCCCCATCTTCTCGCCAATTAATGCCAGGCGGATATATTCGCGGTCTTTATAACCGTTGCAGACGATGACGCTACGGGTCa          >  1:6030337/1‑93 (MQ=255)
                                                                                aTAGACCTTGCGCCCCATCTTCTCGCCAATTAATGCCAGGCGGATATATTCGCGGTCTTTATAACCGTTGCAGACGATGACGCTACGGGTCAt         <  2:5259974/93‑1 (MQ=255)
                                                                                 tAGACCTTGCGCCCCATCTTCTCGCCAATTAATGCCAGGCGGATATATTCGCGGTCTTTATAACCGTTGCAGACGATGACGCTACGGGTCATg        >  2:7140168/1‑93 (MQ=255)
                                                                                 tAGACCTTGCGCCCCATCTTCTCGCCAATTAATGCCAGGCGGATATATTCGCGGTCTTTATAACCGTTGCAGACGATGACGCTACGGGTCATg        <  2:4597048/93‑1 (MQ=255)
                                                                                 tAGACCTTGCGCCCCATCTTCTCGCCAATTAATGCCAGGCGGATATATTCGCGGTCTTTATAACCGTTGCAGACGATGACGCTACGGGTCATg        >  2:505526/1‑93 (MQ=255)
                                                                                  aGACCTTGCGCCCCATCTTCTCGCCAATTAATGCCAGGCGGATATATTCGCGGTCTTTATAACCGTTGCAGACGATGACGCTACGGGTCATGc       <  1:4698258/93‑1 (MQ=255)
                                                                                    aCCTTGCGCCCCATCTTCTCGCCAATTAATGCCAGGCGGATATATTCGCGGTCTTTATAACCGTTGCAGACGATGACGCTACGGGTCATGCCa     >  1:7514650/1‑93 (MQ=255)
                                                                                     ccTTGCGCCCCATCTTCTCGCCAATTAATGCCAGGCGGATATATTCGCGGTCTTTATAACCGTTGCAGACGATGACGCTACGGGTCATGCCAg    <  2:5558001/93‑1 (MQ=255)
                                                                                     ccTTGCGCCCCATCTTCTCGCCAATTAATGCCAGGCGGATATATTCGCGGTCTTTATAACCGTTGCAGACGATGACGCTACGGGTCATGCCAg    >  1:5757245/1‑93 (MQ=255)
                                                                                       ttGCGCCCCATCTTCTCGCCAATTAATGCCAGGCGGATATATTCGCGGTCTTTATAACCGTTGCAGACGATGACGCTACGGGTCATGCCAGCa  <  1:6340113/93‑1 (MQ=255)
                                                                                          |                                                                                         
ACGCCCAGACGAGGAACGACATTCAGACGTTCTGCTTCATCCAGCACAATGGCGATTTCTGACATCTTCTCAATGACCAGATAGACCTTGTGCCCCATCTTCTCGCCAATTAATGCCAGGCGGATATATTCGCGGTCTTTATAACCGTTGCAGACGATGACGCTACGGGTCATGCCAGCA  >  NC_000913/3083269‑3083448

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output Only 200 of 204 total aligned reads displayed.
CGCACGCCCAGACGAGGAACGACATTCAGACGTTCTGCTTCATCCAGCACAATGGCGATTTCTGACATCTTCTCAATGACCAGATAGACCTTGTGCCCCATCTTCTCGCCAATTAATGCCAGGCGGATATATTCGCGGTCTTTATAACCGTTGCAGACGATGACGCTACGGGTCATGCCAGCATGTG  >  NC_000913/3083266‑3083452
                                                                                             |                                                                                             
CGCACGCCCAGACGAGGAACGACATTCAGACGTTCTGCTTCATCCAGCACAATGGCGATTTCTGACATCTTCTCAATGACCAGATAGACCTTGC                                                                                               >  SRR4302543.7523178/1‑94 (MQ=60)
  CACGCCCAGACGAGGAACGACATTCAGACGTTCTGCTTCATCCAGCACAATGGCGATTTCTGACATCTTCTCAATGACCAGATAGACCTTGCGC                                                                                             >  SRR4302543.2236199/1‑94 (MQ=60)
   ACGCCCAGACGAGGAACGACATTCAGACGTTCTGCTTCATCCAGCACAATGGCGATTTCTGACATCTTCTCAATGACCAGATAGACCTTGCGCC                                                                                            >  SRR4302543.1225130/1‑94 (MQ=60)
    CGCCCAGACGAGGAACGACATTCAGACGTTCTGCTTCATCCAGCACAATGGCGATTTCTGACATCTTCTCAATGACCAGATAGACCTTGCGCCC                                                                                           <  SRR4302543.654192/94‑1 (MQ=60)
     GCCCAGACGAGGAACGACATTCAGACGTTCTGCTTCATCCAGCACAATGGCGATTTCTGACATCTTCTCAATGACCAGATAGACCTTGCGCCCC                                                                                          >  SRR4302543.2896859/1‑94 (MQ=60)
      CCCAGACGAGGAACGACATTCAGACGTTCTGCTTCATCCAGCACAATGGCGATTTCTGACATCTTCTCAATGACCAGATAGACCTTGCGCCCCA                                                                                         >  SRR4302543.5390276/1‑94 (MQ=60)
      CCCAGACGAGGAAAGACATTCAGACGTTCTGCTTCATCCAGCACAATGGCGATTTCTGACATCTTCTCAATGACCAGATAGACCTTGCGCCCCA                                                                                         <  SRR4302543.7986835/94‑1 (MQ=60)
       CCAGACGAGGAACGACATTCAGACGTTCTGCTTCATCCAGCACAATGGCGATTTCTGACATCTTCTCAATGACCAGATAGACCTTGCGCCCCAT                                                                                        >  SRR4302543.6551291/1‑94 (MQ=60)
       CCAGACGAGGAACGACATTCAGACGTTCTGCTTCATCCAGCACAATGGCGATTTCTGACATCTTCTCAATGACCAGATAGACCTTGCGCCCCAT                                                                                        >  SRR4302543.4639906/1‑94 (MQ=60)
       CCAGACGAGGAACGACATTCAGACGTTCTGCTTCATCCAGCACAATGGCGATTTCTGACATCTTCTCAATGACCAGATAGACCTTGCGCCCCAT                                                                                        >  SRR4302543.4434385/1‑94 (MQ=60)
       CCAGACGAGGAACGACATTCAGACGTTCTGCTTCATCCAGCACAATGGCGATTTCTGACATCTTCTCAATGACCAGATAGACCTTGCGCCCCAT                                                                                        <  SRR4302543.2757075/94‑1 (MQ=60)
       CCAGACGAGGAACGACATTCAGACGTTCTGCTTCATCCAGCACAATGGCGATTTCTGACATCTTCTCAATGACCAGATAGACCTTGCGCCCCAT                                                                                        <  SRR4302543.2757076/94‑1 (MQ=60)
        CAGACGAGGAACGACATTCAGACGTTCTGCTTCATCCAGCACAATGGCGATTTCTGACATCTTCTCAATGACCAGATAGACCTTGCGCCCCATC                                                                                       >  SRR4302543.4241938/1‑94 (MQ=60)
        CAGACGAGGAACGACATTCAGACGTTCTGCTTCATCCAGCACAATGGCGATTTCTGACATCTTCTCAATGACCAGATAGACCTTGCGCCCCATC                                                                                       >  SRR4302543.7640415/1‑94 (MQ=60)
          GACGAGGAACGACATTCAGACGTTCTGCTTCATCCAGCACAATGGCGATTTCTGACATCTTCTCAATGACCAGATAGACCTTGCGCCCCATCTT                                                                                     >  SRR4302543.2328429/1‑94 (MQ=60)
          GACGAGGAACGACATTCAGACGTTCTGCTTCATCCAGCACAATGGCGATTTCTGACATCTTCTCAATGACCAGATAGACCTTGCGCCCCATCTT                                                                                     >  SRR4302543.1501652/1‑94 (MQ=60)
          AACGAGGAACGACATTCAGACGTTCTGCTTCATCCAGCACAATGGCGATTTCTGACATCTTCTCAATGACCAGATAGACCTTGCGCCCCATCTT                                                                                     <  SRR4302543.6109629/94‑1 (MQ=60)
            CGAGGAACGACATTCAGACGTTCTGCTTCATCCAGCACAATGGCGATTTCTGACATCTTCTCAATGACCAGATAGACCTTGCGCCCCATCTTCT                                                                                   <  SRR4302543.6995394/94‑1 (MQ=60)
            CGAGGAACGACATTCAGACGTTCTGCTTCATCCAGCACAATGGCGATTTCTGACATCTTCTCAATGACCAGATAGACCTTGCGCCCCATCTTCT                                                                                   >  SRR4302543.5410354/1‑94 (MQ=60)
            CGAGGAACGACATTCAGACGTTCTGCTTCATCCAGCACAATGGCGATTTCTGACATCTTCTCAATGACCAGATAGACCTTGCGCCCCATCTTCT                                                                                   >  SRR4302543.1588413/1‑94 (MQ=60)
            CGAGGAACGACATTCAGACGTTCTGCTTCATCCAGCACAATGGCGATTTCTGACATCTTCTCAATGACCAGATAGACCTTGCGCCCCATCTTCT                                                                                   <  SRR4302543.1642099/94‑1 (MQ=60)
            CGAGGAACAACATTCAGACGTTCTGCTTCATCCAGCACAATGGCGATTTCTGACATCTTCTCAATGACCAGATAGACCTTGCGCCCCATCTTCT                                                                                   >  SRR4302543.3519594/1‑94 (MQ=60)
             GAGGAACGACATTCAGACGTTCTGCTTCATCCAGCACAATGGCGATTTCTGACATCTTCTCAATGACCAGATAGACCTTGCGCCCCATCTTCTC                                                                                  >  SRR4302543.5598975/1‑94 (MQ=60)
             GAGGAACGACATTCAGACGTTCTGCTTCATCCAGCACAATGGCGATTTCTGACATCTTCTCAATGACCAGATAGACCTTGCGCCCCATCTTCTC                                                                                  <  SRR4302543.573180/94‑1 (MQ=60)
             GAGGAACGACATTCAGACGTTCTGCTTCATCCAGCACAATGGCGATTTCTGACATCTTCTCAATGACCAGATAGACCTTGCGCCCCATCTTCTC                                                                                  <  SRR4302543.7900841/94‑1 (MQ=60)
             CAGGAACGACATTCAGACGTTCTGCTTCATCCAGCACAATGGCGATTTCTGACATCTCCTCAATGACCAGATAGACCTTGCGCCCCATCTTCTC                                                                                  <  SRR4302543.2661519/94‑1 (MQ=60)
              AGGAACGACATTCAGACGTTCTGCTTCATCCAGCACAATGGCGATTTCTGACATCTTCTCAATGACCAGATAGACCTTGCGCCCCATCTTCTCG                                                                                 <  SRR4302543.6703043/94‑1 (MQ=60)
              AGGAACGACATTCAGACGTTCTGCTTCATCCAGCACAATGGCGATTTCTGACATCTTCTCAATGACCAGATAGACCTTGCGCCCCATCTTCTCG                                                                                 >  SRR4302543.5334689/1‑94 (MQ=60)
              AGGAACGACATTCAGACGTTCTGCTTCATCCAGCACAATGGCGATTTCTGACATCTTCTCAATGACCAGATAGACCTTGCGCCCCATCTTCTCG                                                                                 >  SRR4302543.4332977/1‑94 (MQ=60)
               GGAACGACATTCAGACGTTCTGCTTCATCCAGCACAATGGCGATTTCTGACATCTTCTCAATGACCAGATAGACCTTGCGCCCCATCTTCTCGC                                                                                >  SRR4302543.4954889/1‑94 (MQ=60)
               GGAACGACATTCAGACGTTCTGCTTCATCCAGCACAATGGCGATTTCTGACATCTTCTCAATGACCAGATAGACCTTGCGCCCCATCTTCTCGC                                                                                >  SRR4302543.7296198/1‑94 (MQ=60)
                GAACGACATTCAGACGTTCTGCTTCATCCAGCACAATGGCGATTTCTGACATCTTCTCAATGACCAGATAGACCTTGCGCCCCATCTTCTCGCC                                                                               >  SRR4302543.1663042/1‑94 (MQ=60)
                 AACGACATTCAGACGTTCTGCTTCATCCAGCACAATGGCGATTTCTGACATCTTCTCAATGACCAGATAGACCTTGCGCCCCATCTTCTCGCCA                                                                              <  SRR4302543.3315870/94‑1 (MQ=60)
                 AACGACATTCAGACGTTCTGCTTCATCCAGCACAATGGCGATTTCTGACATCTTCTCAATGACCAGATAGACCTTGCGCCCCATCTTCTCGCCA                                                                              <  SRR4302543.7311770/94‑1 (MQ=60)
                 AACGACATTCAGACGTTCTGCTTCATCCAGCACAATGGCGATTTCTGACATCTTCTCAATGACCAGATAGACCTTGCGCCCCATCTTCTCGCCA                                                                              >  SRR4302543.430664/1‑94 (MQ=60)
                   CGACATTCAGACGTTCTGCTTCATCCAGCACAATGGCGATTTCTGACATCTTCTCAATGACCAGATAGACCTTGCGCCCCATCTTCTCGCCAAT                                                                            >  SRR4302543.7037884/1‑94 (MQ=60)
                    GACATTCAGACGTTCTGCTTCATCCAGCACAATGGCGATTTCTGACATCTTCTCAATGACCAGATAGACCTTGCGCCCCATCTTCTCGCCAATT                                                                           >  SRR4302543.4164860/1‑94 (MQ=60)
                     ACATTCAGACGTTCTGCTTCATCCAGCACAATGGCGATTTCTGACATCTTCTCAATGACCAGATAGACCTTGCGCCCCATCTTCTCGCCAATTA                                                                          <  SRR4302543.1292752/94‑1 (MQ=60)
                     ACATTCAGACGTTCTGCTTCATCCAGCACAATGGCGATTTCTGACATCTTCTCAATGACCAGATAGACCTTGCGCCCCATCTTCTCGCCAATTA                                                                          >  SRR4302543.4326892/1‑94 (MQ=60)
                       ATTCAGACGTTCTGCTTCATCCAGCACAATGGCGATTTCTGACATCTTCTCAATGACCAGATAGACCTTGCGCCCCATCTTCTCGCCAATTAAT                                                                        >  SRR4302543.6126623/1‑94 (MQ=60)
                       ATTCAGACGTTCTGCTTCATCCAGCACAATGGCGATTTCTGACATCTTCTCAATGACCAGATAGACCTTGCGCCCCATCTTCTCGCCAATTAAT                                                                        <  SRR4302543.495372/94‑1 (MQ=60)
                       ATTCAGACGTTCTGCTTCATCCAGCACAATGGCGATTTCTGACATCTTCTCAATGACCAGATAGACCTTGCGCCCCATCTTCTCGCCAATTAAT                                                                        <  SRR4302543.6881666/94‑1 (MQ=60)
                       ATTCAGACGTTCTGCTTCATCCAGCACAATGGCGATTTCTGACATCTTCTCAATGACCAGATAGACCTTGCGCCCCATCTTCTCGCCAATTAAT                                                                        >  SRR4302543.209953/1‑94 (MQ=60)
                       ATTCAGACGTTCTGCTTCATCCAGCACAATGGCGATTTCTGACATCTTCTCAATGACCAGATAGACCTTGCGCCCCATCTTCTCGCCAATTAAT                                                                        <  SRR4302543.6917111/94‑1 (MQ=60)
                       ATTCAGACGTTCTGCTTCATCCAGCACAATGGCGATTTCTGACATCTTCTCAATGACCAGATAGACCTTGCGCCCCATCTTCTCGCCAATTAAT                                                                        <  SRR4302543.7208577/94‑1 (MQ=60)
                        TTCAGACGTTCTGCTTCATCCAGCACAATGGCGATTTCTGACATCTTCTCAATGACCAGATAGACCTTGCGCCCCATCTTCTCGCCAATTAATG                                                                       <  SRR4302543.2923584/94‑1 (MQ=60)
                        TTCAGACGTTCTGCTTCATCCAGCACAATGGCGATTTCTGACATCTTCTCAATGACCAGATAGACCTTGCGCCCCATCTTCTCGCCAATTAATG                                                                       <  SRR4302543.3288611/94‑1 (MQ=60)
                        TTCAGACGTTCTGCTTCATCCAGCACAATGGCGATTTCTGACATCTTCTCAATGACCAGATAGACCTTGCGCCCCATCTTCTCGCCAATTAATG                                                                       <  SRR4302543.7835231/94‑1 (MQ=60)
                        TTCAGACGTTCTGCTTCATCCAGCACAATGGCGATTTCTGACATCTTCTCAATGACCAGATAGACCTTGCGCCCCATCTTCTCGCCAATTAATG                                                                       <  SRR4302543.1144277/94‑1 (MQ=60)
                          CAGACGTTCTGCTTCATCCAGCACAATGGCGATTTCTGACATCTTCTCAATGACCAGATAGACCTTGCGCCCCATCTTCTCGCCAATTAATGCC                                                                     <  SRR4302543.5234427/94‑1 (MQ=60)
                          CAGACGTTCTGCTTCATCCAGCACAATGGCGATTTCTGACATCTTCTCAATGACCAGATAGACCTTGCGCCCCATCTTCTCGCCAATTAATGCC                                                                     <  SRR4302543.1210502/94‑1 (MQ=60)
                          CAGACGTTCTGCTTCATCCAGCACAATGGCGATTTCTGACATCTTCTCAATGACCAGATAGACCTTGCGCCCCATCTTCTCGCCAATTAATGCC                                                                     >  SRR4302543.1686432/1‑94 (MQ=60)
                           AGACGTTCTGCTTCATCCAGCACAATGGCGATTTCTGACATCTTCTCAATGACCAGATAGACCTTGCGCCCCATCTTCTCGCCAATTAATGCCA                                                                    <  SRR4302543.5773798/94‑1 (MQ=60)
                           AGACGTTCTGCTTCATCCAGCACAATGGCGATTTCTGACATCTTCTCAATGACCAGATAGACCTTGCGCCCCATCTTCTCGCCAATTAATGCCA                                                                    <  SRR4302543.3482462/94‑1 (MQ=60)
                           AGACGTTCTGCTTCATCCAGCACAATGGCGATTTCTGACATCTTCTCAATGACCAGATAGACCTTGCGCCCCATCTTCTCGCCAATTAATGCCA                                                                    <  SRR4302543.5774012/94‑1 (MQ=60)
                           AGACGTTCTGCTTCATCCAGCACAATGGCGATTTCTGACATCTTCTCAATGACCAGATAGACCTTGCGCCCCATCTTCTCGCCAATTAATGCCA                                                                    >  SRR4302543.5632628/1‑94 (MQ=60)
                           AGACGTTCTGCTTCATCCAGCACAATGGCGATTTCTGACATCTTCTCAATGACCAGATAGACCTTGCGCCCCATCTTCTCGCCAATTAATGCCA                                                                    >  SRR4302543.5602779/1‑94 (MQ=60)
                           AGACGTTCTGCTTCATCCAGCACAATGGCGATTTCTGACATCTTCTCAATGACCAGATAGACCTTGCGCCCCATCTTCTCGCCAATTAATGCCA                                                                    >  SRR4302543.5091740/1‑94 (MQ=60)
                            GACGTTCTGCTTCATCCAGCACAATGGCGATTTCTGACATCTTCTCAATGACCAGATAGACCTTGCGCCCCATCTTCTCGCCAATTAATGCCAG                                                                   >  SRR4302543.5526375/1‑94 (MQ=60)
                            GACGTTCTGCTTCATCCAGCACAATGGCGATTTCTGACATCTTCTCAATGACCAGATAGACCTTGCGCCCCATCTTCTCGCCAATTAATGCCAG                                                                   >  SRR4302543.129003/1‑94 (MQ=60)
                             ACGTTCTGCTTCATCCAGCACAATGTCGATTTCTGACATCTTCTCAATGACCAGATAGACCTTGCGCCCCATCTTCTCGCCAATTAATGCCAGG                                                                  <  SRR4302543.6240342/94‑1 (MQ=60)
                             ACGTTCTGCTTCATCCAGCACAATGGCGATTTCTGACATCTTCTCAATGACCAGATAGACCTTGCGCCCCATCTTCTCGCCAATTAATGCCAGG                                                                  <  SRR4302543.3688624/94‑1 (MQ=60)
                              CGTTCTGCTTCATCCAGCACAATGGCGATTTCTGACATCTTCTCAATGACCAGATAGACCTTGCGCCCCATCTTCTCGCCAATTAATGCCAGGC                                                                 >  SRR4302543.3625367/1‑94 (MQ=60)
                              CGTTCTGCTTCATCCAGCACAATGGCGATTTCTGACATCTTCTCAATGACCAGATAGACCTTGCGCCCCATCTTCTCGCCAATTAATGCCAGGC                                                                 >  SRR4302543.1848915/1‑94 (MQ=60)
                              CGTTCTGCTTCATCCAGCACAATGGCGATTTCTGACAGCTTCTCAATGACCAGATAGACCTTGCGCCCCATCTTCTCGCCAATTAATGCCAGGC                                                                 <  SRR4302543.7654911/94‑1 (MQ=60)
                               GTTCTGCTTCATCCAGCACAATGGCGATTTCTGACATCTTCTCAATGACCAGATAGACCTTGCGCCCCATCTTCTCGCCAATTAATGCCAGGCG                                                                <  SRR4302543.2786046/94‑1 (MQ=60)
                               GTTCTGCTTCATCCAGCACAATGGCGATTTCTGACATCTTCTCAATGACCAGATAGACCTTGCGCCCCATCTTCTCGCCAATTAATGCCAGGCG                                                                >  SRR4302543.3420344/1‑94 (MQ=60)
                               GTTCTGCTTCATCCAGCACAATGGCGATTTCTGACATCTTCTCAATGACCAGATAGACCTTGCGCCCCATCTTCTCGCCAATTAATGCCAGGCG                                                                >  SRR4302543.6433548/1‑94 (MQ=60)
                               GTTCTGCTTCATCCAGCACAATGGCGATTTCTGACATCTTCTCAATGACCAGATAGACCTTGCGCCCCATCTTCTCGCCAATTAATGCCAGGCG                                                                >  SRR4302543.1865948/1‑94 (MQ=60)
                               GTTCTGATTCATCCAGCACAATGGCGATTTCTGACATCTGCTCAATGACTATATAGCCCTTGTGCCCCATCTGTTCGCCAATTAATGCCAGGCG                                                                <  SRR4302543.1445987/94‑1 (MQ=60)
                                TTCTGCTTCATCCAGCACAATGGCGATTTCTGACATCTTCTCAATGACCAGATAGACCTTGCGCCCCATCTTCTCGCCAATTAATGCCAGGCGG                                                               >  SRR4302543.2295919/1‑94 (MQ=60)
                                  CTGCTTCATCCAGCACAATGGCGATTTCTGACATCTTCTCAATGACCAGATAGACCTTGCGCCCCATCTTCTCGCCAATTAATGCCAGGCGGAT                                                             >  SRR4302543.3834880/1‑94 (MQ=60)
                                  CTGCTTCATCCAGCACAATGGCGATTTCTGACATCTTCTCAATGACCAGATAGACCTTGCGCCCCATCTTCTCGCCAATTAATGCCAGGCGGAT                                                             <  SRR4302543.3039119/94‑1 (MQ=60)
                                  CTGCTTCATCCAGCACAATGGCGATTTCTGACATCTTCTCAATGACCAGATAGACCTTGCGCCCCATCTTCTCGCCAATTAATGCCAGGCGGAT                                                             <  SRR4302543.7608223/94‑1 (MQ=60)
                                   TGCTTCATCCAGCACAATGGCGATTTCTGACATCTTCTCAATGACCAGATAGACCTTGCGCCCCATCTTCTCGCCAATTAATGCCAGGCGGATA                                                            <  SRR4302543.6206433/94‑1 (MQ=60)
                                   TGCTTCATCCAGCACAATGGCGATTTCTGACATCTTCTCAATGACCAGATAGACCTTGCGCCCCATCTTCTCGCCAATTAATGCCAGGCGGATA                                                            <  SRR4302543.1091481/94‑1 (MQ=60)
                                    GCTTCATCCAGCACAATGGCGATTTCTGACATCTTCTCAATGACCAGATAGACCTTGCGCCCCATCTTCTCGCCAATTAATGCCAGGCGGATAT                                                           <  SRR4302543.7424711/94‑1 (MQ=60)
                                     CTTCATCCAGCACAATGGCGATTTCTGACATCTTCTCAATGACCAGATAGACCTTGCGCCCCATCTTCTCGCCAATTAATGCCAGGCGGATATA                                                          <  SRR4302543.1038643/94‑1 (MQ=60)
                                     CTTCATCCAGCACAATGGCGATTTCTGACATCTTCTCAATGACCAGATAGACCTTGCGCCCCATCTTCTCGCCAATTAATGCCAGGCGGATATA                                                          <  SRR4302543.1038642/94‑1 (MQ=60)
                                     CTTCATCCAGCACAATGGCGATTTCTGACATCTTCTCAATGACCAGATAGACCTTGCGCCCCATCTTCTCGCCAATTAATGCCAGGCGGATATA                                                          <  SRR4302543.6936819/94‑1 (MQ=60)
                                      TTCATCCAGCACAATGGCGATTTCTGACATCTTCTCAATGACCAGATAGACCTTGCGCCCCATCTTCTCGCCAATTAATGCCAGGCGGATATAT                                                         >  SRR4302543.7264594/1‑94 (MQ=60)
                                       TCATCCAGCACAATGGCGATTTCTGACATCTTCTCAATGACCAGATAGACCTTGCGCCCCATCTTCTCGCCAATTAATGCCAGGCGGATATATT                                                        >  SRR4302543.6902585/1‑94 (MQ=60)
                                       TCATCCAGCACAATGGCGATTTCTGACATCTTCTCAATGACCAGATAGACCTTGCGCCCCATCTTCTCGCCAATTAATGCCAGGCGGATATATT                                                        <  SRR4302543.323862/94‑1 (MQ=60)
                                       TCATCCAGCACAATGGCGATTTCTGACATCTTCTCAATGACCAGATAGACCTTGCGCCCCATCTTCTCGCCAATTAATGCCAGGCGGATATATT                                                        <  SRR4302543.2760277/94‑1 (MQ=60)
                                        CATCCAGCACAATGGCGATTTCTGACATCTTCTCAATGACCAGATAGACCTTGCGCCCCATCTTCTCGCCAATTAATGCCAGGCGGATATATTC                                                       <  SRR4302543.6658289/94‑1 (MQ=60)
                                        CATCCAGCACAATGGCGATTTCTGACATCTTCTCAATGACCAGATAGACCTTGCGCCCCATCTTCTCGCCAATTAATGCCAGGCGGATATATTC                                                       <  SRR4302543.6531136/94‑1 (MQ=60)
                                        CATCCAGCACAATGGCGATTTCTGACATCTTCTCAATGACCAGATAGACCTTGCGCCCCATCTTCTCGCCAATTAATGCCAGGCGGATATATTC                                                       >  SRR4302543.6594707/1‑94 (MQ=60)
                                        CATCCAGCACAATGGCGATTTCTGACATCTTCTCAATGACCAGATAGACCTTGCGCCCCATCTTCTCGCCAATTAATGCCAGGCGGATATATTC                                                       <  SRR4302543.4135161/94‑1 (MQ=60)
                                         ATCCAGCACAATGGCGATTTCTGACATCTTCTCAATGACCAGATAGACCTTGCGCCCCATCTTCTCGCCAATTAATGCCAGGCGGATATATTCG                                                      <  SRR4302543.7229343/94‑1 (MQ=60)
                                         ATCCAGCACAATGGCGATTTCTGACATCTTCTCAATGACCAGATAGACCTTGCGCCCCATCTTCTCGCCAATTAATGCCAGGCGGATATATTCG                                                      <  SRR4302543.5529181/94‑1 (MQ=60)
                                           CCAGCACAATGGCGATTTCTGACATCTTCTCAATGACCAGATAGACCTTGCGCCCCATCTTCTCGCCAATTAATGCCAGGCGGATATATTCGCG                                                    >  SRR4302543.2024802/1‑94 (MQ=60)
                                           CCAGCACAATGGCGATTTCTGACATCTTCTCAATGACCAGATAGACCTTGCGCCCCATCTTCTCGCCAATTAATGCCAGGCGGATATATTCGCG                                                    >  SRR4302543.4979544/1‑94 (MQ=60)
                                             AGCACAATGGCGATTTCTGACATCTTCTCAATGACCAGATAGACCTTGCGCCCCATCTTCTCGCCAATTAATGCCAGGCGGATATATTCGCGGT                                                  >  SRR4302543.6858737/1‑94 (MQ=60)
                                             AGCACAATGGCGATTTCTGACATCTTCTCAATGACCAGATAGACCTTGCGCCCCATCTTCTCGCCAATTAATGCCAGGCGGATATATTCGCGGT                                                  <  SRR4302543.6656588/94‑1 (MQ=60)
                                             AGCACAATGGCGATTTCTGACATCTTCTCAATGACCAGATAGACCTTGCGCCCCATCTTCTCGCCAATTAATGCCAGGCGGATATATTCGCGGT                                                  >  SRR4302543.5883156/1‑94 (MQ=60)
                                             AGCACAATGGCGATTTCTGACATCTTCTCAATGACCAGATAGACCTTGCGCCCCATCTTCTCGCCAATTAATGCCAGGCGGATATATTCGCGGT                                                  >  SRR4302543.6858738/1‑94 (MQ=60)
                                               CACAATGGCGATTTCTGACATCTTCTCAATGACCAGATAGACCTTGCGCCCCATCTTCTCGCCAATTAATGCCAGGCGGATATATTCGCGGTCT                                                >  SRR4302543.673283/1‑94 (MQ=60)
                                               CACAATGGCGATTTCTGACATCTTCTCAATGACCAGATAGACCTTGCGCCCCATCTTCTCGCCAATTAATGCCAGGCGGATATATTCGCGGTCT                                                >  SRR4302543.1430910/1‑94 (MQ=60)
                                               CACAATGGCGATTTCTGACATCTTCTCAATGACCAGATAGACCTTGCGCCCCATCTTCTCGCCAATTAATGCCAGGCGGATATATTCGCGGTCT                                                >  SRR4302543.3842006/1‑94 (MQ=60)
                                               CACAATGGCGATTTCTGACATCTTCTCAATGACCAGATAGACCTTGCGCCCCATCTTCTCGCCAATTAATGCCAGGCGGATATATTCGCGGTCT                                                >  SRR4302543.7215195/1‑94 (MQ=60)
                                               CACAATGGCGATTTCTGACATCTTCTCAATGACCAGATAGACCTTGCGCCCCATCTTCTCGCCAATTAATGCCAGGCGGATATATTCGCGGTCT                                                <  SRR4302543.3261873/94‑1 (MQ=60)
                                               CACAATGGCGATTTCTGACATCTTCTCAATGACCAGATAGACCTTGCGCCCCATCTTCTCGCCAATTAATGCCAGGCGGATATATTCGCGGTCT                                                <  SRR4302543.1985002/94‑1 (MQ=60)
                                                 CAATGGCGATTTCTGACATCTTCTCAATGACCAGATAGACCTTGCGCCCCATCTTCTCGCCAATTAATGCCAGGCGGATATATTCGCGGTCTTT                                              >  SRR4302543.4997643/1‑94 (MQ=60)
                                                 CAATGGCGATTTCTGACATCTTCTCAATGACCAGATAGACCTTGCGCCCCATCTTCTCGCCAATTAATGCCAGGCGGATATATTCGCGGTCTTT                                              >  SRR4302543.1274953/1‑94 (MQ=60)
                                                 CAATGGCGATTTCTGACATCTTCTCAATGACCAGATAGACCTTGCGCCCCATCTTCTCGCCAATTAATGCCAGGCGGATATATTCGCGGTCTTT                                              >  SRR4302543.7145503/1‑94 (MQ=60)
                                                 CAATGGCGATTTCTGACATCTTCTCAATGACCAGATAGACCTTGCGCCCCATCTTCTCGCCAATTAATGCCAGGCGGATATATTCGCGGTCTTT                                              >  SRR4302543.4093077/1‑94 (MQ=60)
                                                  AATGGCGATTTCTGACATCTTCTCAATGACCAGATAGACCTTGCGCCCCATCTTCTCGCCAATTAATGCCAGGCGGATATATTCGCGGTCTTTA                                             <  SRR4302543.7338149/94‑1 (MQ=60)
                                                    TGGCGATTTCTGACATCTTCTCAATGACCAGATAGACCTTGCGCCCCATCTTCTCGCCAATTAATGCCAGGCGGATATATTCGCGGTCTTTATA                                           >  SRR4302543.2159536/1‑94 (MQ=60)
                                                    TGGCGATTTCTGACATCTTCTCAATGACCAGATAGACCTTGCGCCCCATCTTCTCGCCAATTAATGCCAGGCGGATATATTCGCGGTCTTTATA                                           <  SRR4302543.348116/94‑1 (MQ=60)
                                                    TGGCGATTTCTGACATCTTCTCAATGACCAGATAGACCTTGCGCCCCATCTTCTCGCCAATTAATGCCAGGCGGATATATTCGCGGTCTTTATA                                           <  SRR4302543.3867562/94‑1 (MQ=60)
                                                     GGCGATTTCTGACATCTTCTCAATGACCAGATAGACCTTGTGCCCCATCTTCTCGCCAATTAATGCCAGGCGGATATATTCGCGGTCTTTATAA                                          <  SRR4302543.6567365/94‑1 (MQ=60)
                                                     GGCGATTTCTGACATCTTCTCAATGACCAGATAGACCTTGCGCCCCATCTTCTCGCCAATTAATGCCAGGCGGATATATTCGCGGTCTTTATAA                                          <  SRR4302543.4326632/94‑1 (MQ=60)
                                                      GCGATTTCTGACATCTTCTCAATGACCAGATAGCCCTTGCGCCCCATCTTCTCGCCAATTAATGCCAGGCGGATATATTCGCGGTCTTTATAAC                                         <  SRR4302543.2441525/94‑1 (MQ=60)
                                                      GCGATTTCTGACATCTTCTCAATGACCAGATAGACCTTGCGCCCCATCTTCTCGCCAATTAATGCCAGGCGGATATATTCGCGGTCTTTATAAC                                         <  SRR4302543.5015997/94‑1 (MQ=60)
                                                      GCGATTTCTGACATCTTCTCAATGACCAGATAGACCTTGCGCCCCATCTTCTCGCCAATTAATGCCAGGCGGATATATTCGCGGTCTTTATAAC                                         <  SRR4302543.177766/94‑1 (MQ=60)
                                                      GCGATTTCTGACATCTTCTCAATGACCAGATAGACCTTGCGCCCCATCTTCTCGCCAATTAATGCCAGGCGGATATATTCGCGGTCTTTATAAC                                         <  SRR4302543.2066334/94‑1 (MQ=60)
                                                      GCGATTTCTGACATCTTCTCAATGACCAGATAGACCTTGCGCCCCATCTTCTCGCCAATTAATGCCAGGCGGATATATTCGCGGTCTTTATAAC                                         >  SRR4302543.1973211/1‑94 (MQ=60)
                                                       gcATTTCTGACATCTTCTCAATGACCAGATAGACCTTGCGCCCCATCTTCTCGCCAATTAATGCCAGGCGGATATATTCGCGGTCTTTATAACC                                        <  SRR4302543.2085/92‑1 (MQ=60)
                                                       GGATTTCTGACATCTTCTCAATGACCAGATAGACCTTGCGCCCCATCTGCTCGCCATTTAATGCCAGGCGGATATATTCGCGGTCTTTATAACC                                        <  SRR4302543.5737947/94‑1 (MQ=60)
                                                       CGATTTCTGACATCTTCTCAATGACCAGATAGACCTTGCGCCCCATCTTCTCGCCAATTAATGCCAGGCGGATATATTCGCGGTCTTTATAACC                                        >  SRR4302543.3659533/1‑94 (MQ=60)
                                                       CGATTTCTGACATCTTCTCAATGACCAGATAGACCTTGCGCCCCATCTTCTCGCCAATTAATGCCAGGCGGATATATTCGCGGTCTTTATAACC                                        <  SRR4302543.3078628/94‑1 (MQ=60)
                                                       CGATTTCTGACATCTTCTCAATGACCAGATAGACCTTGCGCCCCATCTTCTCGCCAATTAATGCCAGGCGGATATATTCGCGGTCTTTATAACC                                        <  SRR4302543.6636069/94‑1 (MQ=60)
                                                       CGATTTCTGACATCTTCTCAATGACCAGATAGACCTTGCGCCCCATCTTCTCGCCAATTAATGCCAGGCGGATATATTCGCGGTCTTTATAACC                                        <  SRR4302543.5043959/94‑1 (MQ=60)
                                                       CGATTTCTGACATCTTCTCAATGACCAGATAGACCTTGCGCCCCATCTTCTCGCCAATTAATGCCAGGCGGATATATTCGCGGTCTTTATAACC                                        <  SRR4302543.1660043/94‑1 (MQ=60)
                                                       CGATTTCTGACATCTTCTCAATGACCAGATAGACCTTGCGCCCCATCTTCTCGCCAATTAATGCCAGGCGGATATATTCGCGGTCTTTATAACC                                        <  SRR4302543.744075/94‑1 (MQ=60)
                                                       CGATTTCTGACATCTTCTCAATGACCAGATAGACCTTGCGCCCCATCTTCTCGCCAATTAATGCCAGGCGGATATATTCGCGGTCTTTATAACC                                        <  SRR4302543.6210246/94‑1 (MQ=60)
                                                        GATTTCTGACATCTTCTCAATGACCAGATAGACCTTGCGCCCCATCTTCTCGCCAATTAATGCCAGGCGGATATATTCGCGGTCTTTATAACCG                                       >  SRR4302543.2246006/1‑94 (MQ=60)
                                                        GATTTCTGACATCTTCTCAATGACCAGATAGACCTTGCGCCCCATCTTCTCGCCAATTAATGCCAGGCGGATATATTCGCGGTCTTTATAACCG                                       <  SRR4302543.3250508/94‑1 (MQ=60)
                                                         ATTTCTGACATCTTCTCAATGACCAGATAGACCTTGCGCCCCATCTTCTCGCCAATTAATGCCAGGCGGATATATTCGCGGTCTTTATAACCGT                                      <  SRR4302543.7152850/94‑1 (MQ=60)
                                                          TTTCTGACATCTTCTCAATGACCAGATAGACCTTGCGCCCCATCTTCTCGCCAATTAATGCCAGGCGGATATATTCGCGGTCTTTATAACCGTT                                     <  SRR4302543.3651683/94‑1 (MQ=60)
                                                           TTCTGACATCTTCTCAATGACCAGATAGACCTTGCGCCCCATCTTCTCGCCAATTAATGCCAGGCGGATATATTCGCGGTCTTTATAACCGTTG                                    >  SRR4302543.4736249/1‑94 (MQ=60)
                                                           TTCTGACATCTTCTCAATGACCAGATAGACCTTGCGCCCCATCTTCTCGCCAATTAATGCCAGGCGGATATATTCGCGGTCTTTATAACCGTTG                                    <  SRR4302543.2750956/94‑1 (MQ=60)
                                                            TCTGACATCTTCTCAATGACCAGATAGACCTTGCGCCCCATCTTCTCGCCAATTAATGCCAGGCGGATATATTCGCGGTCTTTATAACCGTTGC                                   >  SRR4302543.2803842/1‑94 (MQ=60)
                                                              TGACATCTTCTCACTAACCAGAAAGACCTTGCGCCCCATCTACTCGCCAATTAATGCCAGGCGAATATATTCGCGGTCTTTAAAACCGTTGCAG                                 <  SRR4302543.7083065/94‑1 (MQ=60)
                                                              TGACATCTTCTCAATGACCAGATAGACCTTGCGCCCCATCTTCTCGCCAATTAATGCCAGGCGGATATATTCGCGGTCTTTATAACCGTTGCAG                                 >  SRR4302543.4782852/1‑94 (MQ=60)
                                                              TGACATCTTCTCAATGACCAGATAGACCTTGCGCCCCATCTTCTCGCCAATTAATGCCAGGCGGATATATTCGCGGTCTTTATAACCGTTGCAG                                 >  SRR4302543.2639388/1‑94 (MQ=60)
                                                               GACATCTTCTCAATGACCAGATAGACCTTGCGCCCCATCTTCTCGCCAATTAATGCCAGGCGGATATATTCGCGGTCTTTATAACCGTTGCAGA                                >  SRR4302543.3389693/1‑94 (MQ=60)
                                                                ACATCTTCTCAATGACCAGATAGACCTTGCGCCCCATCTTCTCGCCAATTAATGCCAGGCGGATATATTCGCGGTCTTTATAACCGTTGCAGAC                               <  SRR4302543.3748194/94‑1 (MQ=60)
                                                                 CATCTTCTCAATGACCAGATAGACCTTGCGCCCCATCTTCTCGCCAATTAATGCCAGGCGGATATATTCGCGGTCTTTATAACCGTTGCAGACG                              >  SRR4302543.6671147/1‑94 (MQ=60)
                                                                  ATCTTCTCAATGACCAGATAGACCTTGCGCCCCATCTTCTCGCCAATTAATGCCAGGCGGATATATTCGCGGTCTTTATAACCGTTGCAGACGA                             >  SRR4302543.291092/1‑94 (MQ=60)
                                                                  ATCTTCTCAATGACCAGATAGACCTTGCGCCCCATCTTCTCGCCAATTAATGCCAGGCGGATATATTCGCGGTCTTTATAACCGTTGCAGACGA                             >  SRR4302543.3368048/1‑94 (MQ=60)
                                                                    CTTCTCAATGACCAGATAGACCTTGCGCCCCATCTTCTCGCCAATTAATGCCAGGCGGATATATTCGCGGTCTTTATAACCGTTGCAGACGATG                           <  SRR4302543.3600781/94‑1 (MQ=60)
                                                                    CTTCTCAATGACCAGATAGACCTTGCGCCCCATCTTCTCGCCAATTAATGCCAGGCGGATATATTCGCGGTCTTTATAACCGTTGCAGACGATG                           <  SRR4302543.4147435/94‑1 (MQ=60)
                                                                    CTTCTCAATGACCAGATAGACCTTGCGCCCCATCTTCTCGCCAATTAATGCCAGGCGGATATATTCGCGGTCTTTATAACCGTTGCAGACGATG                           >  SRR4302543.5524599/1‑94 (MQ=60)
                                                                     TTCTCAATGACCAGATAGACCTTGCGCCCCATCTTCTCGCCAATTAATGCCAGGCGGATATATTCGCGGTCTTTATAACCGTTGCAGACGATGA                          <  SRR4302543.804681/94‑1 (MQ=60)
                                                                     TTCTCAATGACCAGATAGACCTTGCGCCCCATCTTCTCGCCAATTAATGCCAGGCGGATATATTCGCGGTCTTTATAACCGTTGCAGACGATGA                          <  SRR4302543.20691/94‑1 (MQ=60)
                                                                      TCTCAATGACCAGATAGACCTTGCGCCCCATCTTCTCGCCAATTAATGCCAGGCGGATATATTCGCGGTCTTTATAACCGTTGCAGACGATGAC                         <  SRR4302543.1720193/94‑1 (MQ=60)
                                                                      TCTCAATGACCAGATAGACCTTGCGCCCCATCTTCTCGCCAATTAATGCCAGGCGGATATATTCGCGGTCTTTATAACCGTTGCAGACGATGAC                         <  SRR4302543.1998222/94‑1 (MQ=60)
                                                                      TCTCAATGACCAGATAGACCTTGCGCCCCATCTTCTCGCCAATTAATGCCAGGCGGATATATTCGCGGTCTTTATAACCGTTGCAGACGATGAC                         <  SRR4302543.6239988/94‑1 (MQ=60)
                                                                      TCTCAATGACCAGATAGACCTTGCGCCCCATCTTCTCGCCAATTAATGCCAGGCGGATATATTCGCGGTCTTTATAACCGTTGCAGACGATGAC                         <  SRR4302543.1270839/94‑1 (MQ=60)
                                                                      TCTCAATGACCAGATAGACCTTGCGCCCCATCTTCTCGCCAATTAATGCCAGGCGGATATATTCGCGGTCTTTATAACCGTTGCAGACGATGAC                         <  SRR4302543.1354801/94‑1 (MQ=60)
                                                                       CTCAATGACCAGATAGACCTTGCGCCCCATCTTCTCGCCAATTAATGCCAGGCGGATATATTCGCGGTCTTTATAACCGTTGCAGACGATGACG                        <  SRR4302543.5958605/94‑1 (MQ=60)
                                                                       CTCAATGACCAGATAGACCTTGCGCCCCATCTTCTCGCCAATTAATGCCAGGCGGATATATTCGCGGTCTTTATAACCGTTGCAGACGATGACG                        <  SRR4302543.471923/94‑1 (MQ=60)
                                                                       CTCAATGACCAGATAGACCTTGCGCCCCATCTTCTCGCCAATTAATGCCAGGCGGATATATTCGCGGTCTTTATAACCGTTGCAGACGATGACG                        >  SRR4302543.1755301/1‑94 (MQ=60)
                                                                       CTCAATGACCAGATAGACCTTGCGCCCCATCTTCTCGCCAATTAATGCCAGGCGGATATATTCGCGGTCTTTATAACCGTTGCAGACGATGACG                        <  SRR4302543.5040145/94‑1 (MQ=60)
                                                                       CTCAATGACCAGATAGACCTTGCGCCCCATCTTCTCGCCAATTAATGCCAGGCGGATATATTCGCGGTCTTTATAACCGTTGCAGACGATGACG                        >  SRR4302543.2723281/1‑94 (MQ=60)
                                                                       CTCAATGACCAGATAGACCTTGCGCCCCATCTTCTCGCCAATTAATGCCAGGCGGATATATTCGCGGTCTTTATAACCGTTGCAGACGATGACG                        <  SRR4302543.4057705/94‑1 (MQ=60)
                                                                        TCAGTGACCAGATAGACCTTGCGCCCCATCTTCTCGCCAATTAATGCCAGGCGGATATATTCGCGGTCTTTATAACCGTTGCAGACGATGACGC                       >  SRR4302543.7463174/1‑94 (MQ=60)
                                                                        TCAATGACCAGATAGACCTTGCGCCCCATCTTCTCGCCAATTAATGCCAGGCGGATATATTCGCGGTCTTTATAACCGTTGCAGACGATGACGC                       >  SRR4302543.1037256/1‑94 (MQ=60)
                                                                        TCAATGACCAGATAGACCTGGCGCCCCATCTTCTCGCCAATTAATGCCATGCGGATATATTCGCGGTCTTTATAACCGTTGCAGACGATGACGC                       <  SRR4302543.3328063/94‑1 (MQ=60)
                                                                         CAATGACCAGATAGACCTTGCGCCCCATCTTCTCGCCAATTAATGCCAGGCGGATATATTCGCGGTCTTTATAACCGTTGCAGACGATGACGCT                      <  SRR4302543.4573370/94‑1 (MQ=60)
                                                                         CAATGACCAGATAGACCTTGCGCCCCATCTTCTCGCCAATTAATGCCAGGCGGATATATTCGCGGTCTTTATAACCGTTGCAGACGATGACGCT                      >  SRR4302543.802238/1‑94 (MQ=60)
                                                                          AATGACCAGATAGACCTTGCGCCCCATCTTCTCGCCAATTAATGCCAGGCGGATATATTCGCGGTCTTTATAACCGTTGCAGACGATGACGCTA                     <  SRR4302543.1815560/94‑1 (MQ=60)
                                                                          AATGACCAGATAGACCTTGCGCCCCATCTTCTCGCCAATTAATGCCAGGCGGATATATTCGCGGTCTTTATAACCGTTGCAGACGATGACGCTA                     >  SRR4302543.5125373/1‑94 (MQ=60)
                                                                           ATGACCAGATAGACCTTGCGCCCCATCTTCTCGCCAATTAATGCCAGGCGGATATATTCGCGGTCTTTATAACCGTTGCAGACGATGACGCTAC                    <  SRR4302543.1949414/94‑1 (MQ=60)
                                                                           ATGACCAGATAGACCTTGCGCCCCATCTTCTCGCCAATTAATGCCAGGCGGATATATTCGCGGTCTTTATAACCGTTGCAGACGATGACGCTAC                    <  SRR4302543.524927/94‑1 (MQ=60)
                                                                           ATGACCAGATAGACCTTGCGCCCCATCTTCTCGCCAATTAATGCCAGGCGGATATATTCGCGGTCTTTATAACCGTTGCAGACGATGACGCTAC                    >  SRR4302543.1150119/1‑94 (MQ=60)
                                                                           ATGACCAGATAGACCGTGCGCCCCATCTTCTCGCCAATTAATGCCAGGCGGATATATTCGCGGTCTTTATAACCGTTGCAGACGATGACGCTAC                    <  SRR4302543.4465993/94‑1 (MQ=60)
                                                                            TGACCAGATAGACCTTGCGCCCCATCTTCTCGCCAATTAATGCCAGGCGGATATATTCGCGGTCTTTATAACCGTTGCAGACGATGACGCTACG                   >  SRR4302543.3305885/1‑94 (MQ=60)
                                                                            TGACCAGATAGACCTTGCGCCCCATCTTCTCGCCAATTAATGCCAGGCGGATATATTCGCGGTCTTTATAACCGTTGCAGACGATGACGCTACG                   <  SRR4302543.6835042/94‑1 (MQ=60)
                                                                             GACCAGATAGACCTTGCGCCCCATCTTCTCGCCAATTAATGCCAGGCGGATATATTCGCGGTCTTTATAACCGTTGCAGACGATGACGCTACGG                  <  SRR4302543.4631656/94‑1 (MQ=60)
                                                                               CCAGATAGACCTTGCGCCCCATCTTCTCGCCAATTAATGCCAGGCGGATATATTCGCGGTCTTTATAACCGTTGCAGACGATGACGCTACGGGT                >  SRR4302543.5474088/1‑94 (MQ=60)
                                                                               CCAGATAGACCTTGCGCCCCATCTTCTCGCCAATTAATGCCAGGCGGATATATTCGCGGTCTTTATAACCGTTGCAGACGATGACGCTACGGGT                >  SRR4302543.1413656/1‑94 (MQ=60)
                                                                                 AGATAGACCTTGCGCCCCATCTTCTCGCCAATTAATGCCAGGCGGATATATTCGCGGTCTTTATAACCGTTGCAGACGATGACGCTACGGGTCA              >  SRR4302543.642797/1‑94 (MQ=60)
                                                                                 AGATAGACCTTGCGCCCCATCTTCTCGCCAATTAATGCCAGGCGGATATATTCGCGGTCTTTATAACCGTTGCAGACGATGACGCTACGGGTCA              <  SRR4302543.1647460/94‑1 (MQ=60)
                                                                                 AGATAGACCTTGCGCCCCATCTTCTCGCCAATTAATGCCAGGCGGATATATTCGCGGTCTTTATAACCGTTGCAGACGATGACGCTACGGGTCA              <  SRR4302543.7140702/94‑1 (MQ=60)
                                                                                 AGATAGACCTTGCGCCCCATCTTCTCGCCAATTAATGCCAGGCGGATATATTCGCGGTCTTTATAACCGTTGCAGACGATGACGCTACGGGTCA              >  SRR4302543.6086745/1‑94 (MQ=60)
                                                                                 AGATAGACCTTGCGCCCCATCTTCTCGCCAATTAATGCCAGGCGGATATATTCGCGGTCTTTATAACCGTTGCAGACGATGACGCTACGGGTCA              <  SRR4302543.3077564/94‑1 (MQ=60)
                                                                                  TATAGACCTTGCGCCCCATCTTCTCGCCAATTAATGCCAGGCGGATATATTCGCGGTCTTTATAACCGTTGCAGACGATGACGCTACGGGTCAT             <  SRR4302543.5445333/94‑1 (MQ=60)
                                                                                  GATAGACCTTGCGCCCCATCTTCTCGCCAATTAATGCCAGGCGGATATATTCGCGGTCTTTATAACCGTTGCAGACGATGACGCTACGGGTCAT             >  SRR4302543.1276056/1‑94 (MQ=60)
                                                                                   ATAGACCTTGCGCCCCATCTTCTCGCCAATTAATGCCAGGCGGATATATTCGCGGTCTTTATAACCGTTGCAGACGATGACGCTACGGGTCATG            >  SRR4302543.371770/1‑94 (MQ=60)
                                                                                     AGACCTTGCGCCCCATCTTCTCGCCAATTAATGCCAGGCGGATATATTCGCGGTCTTTATAACCGTTGCAGACGATGACGCTACGGGTCATGCC          <  SRR4302543.4741167/94‑1 (MQ=60)
                                                                                     AGACCTTGCGCCCCATCTTCTCGCCAATTAATGCCAGGCGGATATATTCGCGGTCTTTATAACCGTTGCAGACGATGACGCTACGGGTCATGCC          <  SRR4302543.1448788/94‑1 (MQ=60)
                                                                                     AGACCTTGCGCCCCATCTTCTCGCCAATTAATGCCAGGCGGATATATTCGCGGTCTTTATAACCGTTGCAGACGATGACGCTACGGGTCATGCC          >  SRR4302543.82683/1‑94 (MQ=60)
                                                                                     AGACCTTGCGCCCCATCTTCTCGCCAATTAATGCCAGGCGGATATATTCGCGGTCTTTATAACCGTTGCAGACGATGACGCTACGGGTCATGCC          <  SRR4302543.3208829/94‑1 (MQ=60)
                                                                                     AGACCTTGCGCCCCATCTTCTCGCCAATTAATGCCAGGCGGATATATTCGCGGTCTTTATAACCGTTGCAGACGATGACGCTACGGGTCATGCC          >  SRR4302543.3447663/1‑94 (MQ=60)
                                                                                      GACCTTGCGCCCCATCTTCTCGCCAATTAATGCCAGGCGGATATATTCGCGGTCTTTATAACCGTTGCAGACGATGACGCTACGGGTCATGCCA         >  SRR4302543.2058556/1‑94 (MQ=60)
                                                                                      GACCTTGCGCCCCATCTTCTCGCCAATTAATGCCAGGCGGATATATTCGCGGTCTTTATAACCGTTGCAGACGATGACGCTACGGGTCATGCCA         >  SRR4302543.7586412/1‑94 (MQ=60)
                                                                                      GACCTTGCGCCCCATCTTCTCGCCAATTAATGCCAGGCGGATATATTCGCGGTCTTTATAACCGTTGCAGACGATGACGCTACGGGTCATGCCA         >  SRR4302543.6031145/1‑94 (MQ=60)
                                                                                       ACCTTGCGCCCCATCTTCTCGCCAATTAATGCCAGGCGGATATATTCGCGGTCTTTATAACCGTTGCAGACGATGACGCTACGGGTCATGCCAG        <  SRR4302543.1556963/94‑1 (MQ=60)
                                                                                       ACCTTGCGCCCCATCTTCTCGCCAATTAATGCCAGGCGGATATATTCGCGGTCTTTATAACCGTTGCAGACGATGACGCTACGGGTCATGCCAG        >  SRR4302543.3802350/1‑94 (MQ=60)
                                                                                       ACCTTGCGCCCCATCTTCTCGCCAATTAATGCCAGGCGGATATATTCGCGGTCTTTATAACCGTTGCAGACGATGACGCTACGGGTCATGCCAG        >  SRR4302543.5809704/1‑94 (MQ=60)
                                                                                        CCTTGCGCCCCATCTTCTCGCCAATTAATGCCAGGCGGATATATTCGCGGTCTTTATAACCGTTGCAGACGATGACGCTACGGGTCATGCCAGC       >  SRR4302543.726666/1‑94 (MQ=60)
                                                                                        CCTTGCGCCCCATCTTCTCGCCAATTAATGCCAGGCGGATATATTCGCGGTCTTTATAACCGTTGCAGACGATGACGCTACGGGTCATGCCAGC       >  SRR4302543.7240266/1‑94 (MQ=60)
                                                                                        CCTTGCGCCCCATCTTCTCGCCAATTAATGCCAGGCGGATATATTCGCGGTCTTTATAACCGTTGCAGACGATGACGCTACGGGTCATGCCAGC       <  SRR4302543.2306345/94‑1 (MQ=60)
                                                                                          TTGCGCCCCATCTTCTCGCCAATTAATGCCAGGCGGATATATTCGCGGTCTTTATAACCGTTGCAGACGATGACGCTACGGGTCATGCCAGCAT     >  SRR4302543.4859509/1‑94 (MQ=60)
                                                                                          TTGCGCCCCATCTTCTCGCCAATTAATGCCAGGCGGATATATTCGCGGTCTTTATAACCGTTGCAGACGATGACGCTACGGGTCATGCCAGCAT     <  SRR4302543.2966981/94‑1 (MQ=60)
                                                                                          TTGCGCCCCATCTTCTCGCCAATTAATGCCAGGCGGATATATTCGCGGTCTTTATAACCGTTGCAGACGATGACGCTACGGGTCATGCCAGCAT     <  SRR4302543.6399727/94‑1 (MQ=60)
                                                                                            GCGCCCCATCTTCTCGCCAATTAATGCCAGGCGGATATATTCGCGGTCTTTATAACCGTTGCAGACGATGACGCTACGGGTCATGCCAGCATGT   >  SRR4302543.1364493/1‑94 (MQ=60)
                                                                                             CGCCCCATCTTCTCGCCAATTAATGCCAGGCGGATATATTCGCGGTCTTTATAACCGTTGCAGACGATGACGCTACGGGTCATGCCAGCATGTG  >  SRR4302543.2137702/1‑94 (MQ=60)
                                                                                             |                                                                                             
CGCACGCCCAGACGAGGAACGACATTCAGACGTTCTGCTTCATCCAGCACAATGGCGATTTCTGACATCTTCTCAATGACCAGATAGACCTTGTGCCCCATCTTCTCGCCAATTAATGCCAGGCGGATATATTCGCGGTCTTTATAACCGTTGCAGACGATGACGCTACGGGTCATGCCAGCATGTG  >  NC_000913/3083266‑3083452

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑