Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 1696 279.3 15874187 97.8% 15524954 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,089,241 G→A R30H (CGC→CAC)  b2946 → hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,089,2410GA98.9% 1260.2 / ‑2.5 356R30H (CGC→CAC) b2946hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (3/1);  new base A (187/165);  total (190/166)
Fisher's exact test for biased strand distribution p-value = 6.26e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.16e-01

Only 100 of 368 total aligned reads displayed.

CTATGCGTATCCCCCGCATTTATCATCCTGAACCACTGACCAGCCATTCTCACATCGCGCTTTGCGAAGATGCCGCCAACCATATCGGGCGCGTACTGCGCATGGGGCCGGGGCAGGCGTTGCAATTGTTTGACGGTAGCAACCAGGTCTTTGACGCCGAAATTACCAGCGCCAGCAAAA  >  NC_000913/3089151‑3089330
                                                                                          |                                                                                         
 tATGCGTATCCCCCGCATTTATCATCCTGAACCACTGACCAGCCATTCTCACATCGCGCTTTGCGAAGATGCCGCCAACCATATCGGGCACGt                                                                                        <  2:3611107/93‑1 (MQ=255)
 tATGCGTATCCCCCGCATTTATCATCCTGAACCACTGACCAGCCATTCTCACATCGCGCTTTGCGAAGATGCCGCCAACCATATCGGGCACGt                                                                                        >  2:3858089/1‑93 (MQ=255)
   tGCGTATCCCCCGCATTTATCATCCTGAACCACTGACCAGCCATTCTCACATCGCGCTTTGCGAAGATGCCGCCCACCATATCGGGCACGTAc                                                                                      >  2:4197341/1‑93 (MQ=255)
   tGCGTATCCCCCGCATTTATCATCCTGAACCACTGACCAGCCATTCTCACATCGCGCTTTGCGAAGATGCCGCCAACCATATCGGGCACGTAc                                                                                      <  2:1728558/93‑1 (MQ=255)
   tGCGTATCCCCCGCATTTATCATCCTGAACCACTGACCAGCCATTCTCACATCGCGCTTTGCGAAGATGCCGCCAACCATATCGGGCACGTAc                                                                                      <  1:2426692/93‑1 (MQ=255)
   tGCGTATCCCCCGCATTTATCATCCTGAACCACTGACCAGCCATTCTCACATCGCGCTTTGCGAAGATGCCGCCAACCATATCGGGCACGTAc                                                                                      <  1:1903291/93‑1 (MQ=255)
   tGCGTAGCCCCCGCATTTATCATCCTGAACCACTGTCCAGCCATTCTCACATCGCGCTTTGCGAAGATGCCGCCAACCATATCGGGCACGTAc                                                                                      <  1:306208/93‑1 (MQ=255)
      gTATCCCCCGCATTTATCATCCTGAACCACTGACCAGCCATTCTCACATCGCGCTTTGCGAAGATGCCGCCAACCATATCGGGCACGTACTgc                                                                                   >  1:4057747/1‑93 (MQ=255)
      gTATCCCCCGCATGTATCATCCTGAACCACTGACCAGCCATTCTCACATCGCGCTTTGCGAAGATGCCGCCAACCATATCGGGCACGTACTgc                                                                                   <  1:1134428/93‑1 (MQ=255)
         tCCCCCGCATTTATCATCCTGAACCACTGACCAGCCATTCTCACATCGCGCTTTGCGAAGATGCCGCCAACCATATCGGGCACGTACTGCGCa                                                                                >  2:4975699/1‑93 (MQ=255)
         tCCCCCGCATTTATCATCCTGAACCACTGACCAGCCATTCTCACATCGCGCTTTGCGAAGATGCCGCCAACCATATCGGGCACGTACTGCGCa                                                                                <  2:2250249/93‑1 (MQ=255)
           ccccGCATTTATCATCCTGAACCACTGACCAGCCATTCTCACATCGCGCTTTGCGAAGATGCCGCCAACCATATCGGGCACGTACTGCGCATg                                                                              <  1:1038711/93‑1 (MQ=255)
           ccccGCATTTATCATCCTGAACCACTGACCAGCCATTCTCACATCGCGCTTTGCGAAGATGCCGCCAACCATATCGGGCACGTACTGCGCATg                                                                              <  1:3566279/93‑1 (MQ=255)
            cccGCATTTATCATCCTGACCCACTGACCAGCCATTCTCACATCGCGCTTTGCGAAGATGCCGCCAACCATATCGGGCACGTACTGCGCATgg                                                                             <  1:1440335/93‑1 (MQ=255)
            cccGCATTTATCATCCTGAACCACTGACCAGCCATTCTCACATCGCGCTTTGCGAAGATGCCGCCAACCATATCGGGCACGTACTGCGCATgg                                                                             <  2:5111474/93‑1 (MQ=255)
            cccGCATTTATCATCCTGAACCACTGACCAGCCATTCTCACATCGCGCTTTGCGAAGATGCCGCCAACCATATCGGGCACGTACTGCGCATgg                                                                             <  1:2455614/93‑1 (MQ=255)
             ccGCATTTATCATCCTGAACCACTGACCAGCCATTCTCACATCGCGCTTTGCGAAGATGCCGCCAACCATATCGGGCACGTACTGCGCATggg                                                                            <  1:1869610/93‑1 (MQ=255)
             ccGCATTTATCATCCTGAACCACTGACCAGCCATTCTCACATCGCGCTTTGCGAAGATGCCGCCAACCATATCGGGCACGTACTGCGCATggg                                                                            >  1:2681270/1‑93 (MQ=255)
             ccGCATTTATCATCCTGAACCACTGACCAGCCATTCCCACATCGCGCTTTGCGAAGATGCCGCCAACCATATCGGGCACGTACTGCGCATggg                                                                            <  2:4785991/93‑1 (MQ=255)
              cGCATTTATCATCCTGAACCACTGACCAGCCATTCTCACATCGCGCTTTGCGAAGATGCCGCCAACCATATCGGGCACGTACTGCGCATgggg                                                                           <  1:7549095/93‑1 (MQ=255)
              cGCATTTATCATCCTGAACCACTGACCAGCCATTCTCACATCGCGCTTTGCGAAGATGCCGCCAACCATATCGGGCACGTACTGCGCATgggg                                                                           >  1:6510288/1‑93 (MQ=255)
               ccATTTATCATCCTGAACCACTGACCAGCCATTCTCACATCGCGCTTTGCGAAGATGCCGCCAACCATATCGGGCACGTACTGCGCATGGGGc                                                                          <  1:2048292/92‑1 (MQ=255)
                cATTTATCATCCTGAACCACTGACCAGCCATTCTCACATCGCGCTTTGCGAAGATGCCGCCAACCATATCGGGCGCGTACTGCGCATGGGGcc                                                                         >  1:7928501/1‑93 (MQ=255)
                 aTTTATCATCCTGAACCACTGACCAGCCATTCTCACATCGCGCTTTGCGAAGATGCCGCCAACCATATCGGGCACGTACTGCGCATGGGGCCg                                                                        <  1:2108915/93‑1 (MQ=255)
                  tttATCATCCTGAACCACTGACCAGCCATTCTCACATCGCGCTTTGCGAAGATGCCGCCAACCATATCGGGCACGTACTGCGCATGGGGCCgg                                                                       <  1:3491051/93‑1 (MQ=255)
                  tttATCATCCTGAACCACTGACCAGCCATTCTCACATCGCGCTTTGCGAAGATGCCGCCAACCATATCGGGCACGTACTGCGCATGGGGCCgg                                                                       >  1:7316208/1‑93 (MQ=255)
                  tttATCATCCTGAACCACTGACCAGCCATTCTCACATCGCGCTTTGCGAAGATGCCGCCAACCATATCGGGCACGTACTGCGCATGGGGCCgg                                                                       >  2:2468870/1‑93 (MQ=255)
                  tttATCATCCTGAACCACTGACCAGCCATTCTCACATCGCGCTTTGCGAAGATGCCGCCAACCATATCGGGCACGTACTGCGCATGGGGCCgg                                                                       >  2:2101262/1‑93 (MQ=255)
                   ttATCATCCTGAACCACTGACCAGCCATTCTCACATCGCGCTTTGCGAAGATGCCGCCAACCATATCGGGCACGTACTGCGCATGGGGCCggc                                                                      >  1:5200555/1‑92 (MQ=255)
                    tATCATCCTGAACCACTGACCAGCCATTCTCACATCGCGCTTTGCGAAGATGCCGCCAACCATATCGGGCACGTACTGCGCATGGGGCCgggg                                                                     <  1:4975829/93‑1 (MQ=255)
                    tATCATCCTGAACCACTGACCAGCCATTCTCACATCGCGCTTTGCGAAGATGCCGCCAACCATATCGGGCACGTACTGCGCATGGGGCCgggg                                                                     <  1:5572019/93‑1 (MQ=255)
                      tcatcCTGAACCACTGACCAGCCATTCTCACATCGCGCTTTGCGAAGATGCCGCCAACCATATCGGGCACGTACTGCGCATGGGGCCGGGGCa                                                                   >  1:2542145/1‑93 (MQ=255)
                         tcCTGAACCACTGACCAGCCATTCTCACATCGCGCTTTGCGAAGATGCCGCCAACCATATCGGGCACGTACTGTGCATGGGGCCGGGGCAGGc                                                                >  2:7241911/1‑93 (MQ=255)
                         tcCTGAACCACTGACCAGCCATTCTCACATCGCGCTTTGCGAAGATGCCGCCAACCATATCGGGCACGTACTGCGCATGGGGCCGGGGCAGGc                                                                >  2:4880372/1‑93 (MQ=255)
                         tcCTGAACCACTGACCAGCCATTCTCACATCGCGCTTTGCGAAGATGCCGCCAACCATATCGGGCACGTACTGCGCATGGGGCCGGGGCAGGc                                                                >  2:7599932/1‑93 (MQ=255)
                         tcCTGAACCACTGACCAGCCATTCTCACATCGCGCTTTGCGAAGATGCCGCCAACCATATCGGGCACGTACTGCGCATGGGGCCGGGGCAGGc                                                                >  2:3026051/1‑93 (MQ=255)
                         tcCTGAACCACTGACCAGCCATTCTCACATCGCGCTTTGCGAAGATGCCGCCAACCATATCGGGCACGTACTGCGCATGGGGCCGGGGCAGGc                                                                <  2:5533634/93‑1 (MQ=255)
                         tcCTGAACCACTGACCAGCCATTCTCACATCGCGCTTTGCGAAGATGCCGCCAACCATATCGGGCACGTACTGCGCATGGGGCCGGGGCAGGc                                                                <  1:494308/93‑1 (MQ=255)
                          ccTGAACCACTGACCAGCCATTCTCACATCGCGCTTTGCGAAGATGCCGCCAACCATATCGGGCACGTACTGCGCATGGTGCCTGGGCAGGct                                                               >  2:4156518/1‑92 (MQ=255)
                           cTGAACCACTGACCAGCCATTCTCACATCGCGCTTTGCGAAGATGCCGCCAACCATATCGGGCACGTACTGCGCATGGGGCCGGGGCAGGCGt                                                              <  1:1332469/93‑1 (MQ=255)
                           cTGAACCACTGACCAGCCATTCTCACATCGCGCTTTGCGAAGATGCCGCCAACCATATCGGGCACGTACTGCGCATGGGGCCGGGGCAGGCGt                                                              >  1:1182960/1‑93 (MQ=255)
                           cTGAACCACTGACCAGCCATTCTCACATCGCGCTTTGCGAAGATGCCGCCAACCATATCGGGCACGTACTGCGCATGGGGCCGGGGCAGGCGt                                                              >  2:6014544/1‑93 (MQ=255)
                            tGAACCACTGACCAGCCATTCTCACATCGCGCTTTGCGAAGATGCCGCCAACCATATCGGGCACGTACTGCGCATGGGGCCGGGGCAGGCGtt                                                             >  2:4547483/1‑93 (MQ=255)
                            tGAACCACTGACCAGCCATTCTCACATCGCGCTTTGCGAAGATGCCGCCAACCATATCGGGCACGTACTGCGCATGGGGCCGGGGCAGGCGtt                                                             >  1:7101084/1‑93 (MQ=255)
                             gAACCACTGACCAGCCATTCTCACATCGCGCTTTGCGAAGATGCCGCCAACCATATCGGGCACGTACTGCGCATGGGGCCGGGGCAGGCGTTg                                                            >  2:1909970/1‑93 (MQ=255)
                             gAACCACGGACCAGCCATTCTCACATCGCGCTTTGCGAAGATGCCGCCAACCATATCGGGCACGTACTGCGCATGGGGCCGGGGCAGGCGTTg                                                            <  1:2740551/93‑1 (MQ=255)
                              aaCCACTGACCAGCCATTCTCACATCGCGCTTTGCGAAGATGCCGCCAACCATATCGGGCACGTACTGCGCATGGGGCCGGGGCAGGCGTTGc                                                           <  2:6619501/93‑1 (MQ=255)
                               aCCACTGACCAGCCATTCTCACATCGCGCTTTGCGAAGATGCCGCCAACCATATCGGGCACGTACTGCGCATGGGGCCGGGGCAGGCGTTGCa                                                          >  2:3282676/1‑93 (MQ=255)
                               aCCACTGACCAGCCATTCTCACATCGCGCTTTGCGAAGATGCCGCCAACCATATCGGGCACGTACTGCGCATGGGGCCGGGGCAGGCGTTGCa                                                          >  1:5740527/1‑93 (MQ=255)
                               aCCACTGACCAGCCATTCTCACATCGCGCTTTGCGAAGATGCCGCCAACCATATCGGGCACGTACTGCGCATGGGGCCGGGGCAGGCGGTGCa                                                          >  2:2860193/1‑93 (MQ=255)
                                ccACTGACCAGCCATTCTCACATCGCGCTTTGCGAAGATGCCGCCAACCATATCGGGCACGTACTGCGCATGGGGCCGGGGCAGGGGTTGCat                                                         >  2:5803772/1‑92 (MQ=255)
                                ccACTGACCAGCCATTCTCACATCGCGCTTTGCGAAGATGCCGCCAACCATATCGGGCACGTACTGCGCATGGGGCCGGGGCAGGCGTTGCaa                                                         >  2:1409763/1‑93 (MQ=255)
                                 cACTGACCAGCCATTCTCACATCGCGCTTTGCGAAGATGCCGCCAACCATATCGGGCACGTACTGCGCATGGGGCCGGGGCAGGCGTTGCAAt                                                        <  1:4400437/93‑1 (MQ=255)
                                  aCTGACCAGCCATTCTCACATCGCGCTTTGCGAAGATGCCGCCAACCATATCGGGCACGTACTGCGCATGGGGCCGGGGCAGGCGTTGCAAtt                                                       >  2:1810660/1‑93 (MQ=255)
                                   cTGACCAGCCATTCTCACATCGCGCTTTGCGAAGATGCCGCCAACCATATCGGGCACGTACTGCGCATGGGGCCGGGGCAGGCGTTGCAATTg                                                      <  1:4283125/93‑1 (MQ=255)
                                    tGACCAGCCATTCTCACATCGCGCTTTGCGAAGATGCCGCCAACCATATCGGGCACGTACTGCGCATGGGGCCGGGGCAGGCGTTGCAATTGt                                                     >  2:6736127/1‑93 (MQ=255)
                                    tGACCAGCCATTCTCACATCGCGCTTTGCGAAGATGCCGCCAACCATATCGGGCACGTACTGCGCATGGGGCCGGGGCAGGCGTTGCAATTGt                                                     <  2:7858208/93‑1 (MQ=255)
                                     gACCAGCCATTCTCACATCGCGCTTTGCGAAGATGCCGCCAACCATATCGGGCACGTACTGCGCATGGGGCCGGGGCAGGCGTTGCAATTGtt                                                    >  2:6973004/1‑93 (MQ=255)
                                       ccAGCCATTCTCACATCGCGCTTTGCGAAGATGCCGCCAACCATATCGGGCACGTACTGTGCATGGGGCCGGGGCAGGCGTTGCAATTGTTTg                                                  >  1:3196207/1‑93 (MQ=255)
                                       ccAGCCATTCTCACATCGCGCTTTGCGAAGATGCCGCCAACCATATCGGGCACGTACTGCGCATGGGGCCGGGGCAGGCGTTGCAATTGTTTg                                                  >  2:1394705/1‑93 (MQ=255)
                                         aGCCATTCTCACATCGCGCTTTGCGAAGATGCCGCCAACCATATCGGGCACGTACTGCGCATGGGGCCGGGGCAGGCGTTGCAATTGTTTGAc                                                <  1:1142049/93‑1 (MQ=255)
                                          gCCATTCTCACATCGCGCTTTGCGAAGATGCCGCCAACCATATCGGGCACGTACTGCGCATGGGGCCGGGGCAGGCGTTGCAATTGTTTGACg                                               <  2:3509125/93‑1 (MQ=255)
                                          gCCATTCTCACATCGCGCTTTGCGAAGATGCCGCCAACCATATCGGGCACGTACTGCGCATGGGGCCGGGGCAGGCGTTGCAATTGTTTGACg                                               >  1:5869513/1‑93 (MQ=255)
                                           ccATTCTCACATCGCGCTTTGCGAAGATGCCGCCAACCATATCGGGCACGTACTGCGCATGGGGCCGGGGCAGGCGTTGCAATTGTTTGACgg                                              >  2:2877649/1‑93 (MQ=255)
                                            cATTCTCACATCGCGCTTTGCGAAGATGCCGCCAACCATATCGGGCACGTACTGCGCATGGGGCCGGGGCAGGCGTTGCAATTGTTTGACGGt                                             <  2:7184591/93‑1 (MQ=255)
                                              ttCTCACATCGCGCTTTGCGAAGATGCCGCCAACCATATCGGGCACGTACTGCGCATGGGGCCGGGGCAGGCGTTGCAATTGTTTGACGGTAg                                           <  1:176552/93‑1 (MQ=255)
                                              ttCTCACATCGCGCTTTGCGAAGATGCCGCCAACCATATCGGGCACGTACTGCGCATGGGGCCGGGGCAGGCGTTGCAATTGTTTGACGGTAg                                           <  2:378676/93‑1 (MQ=255)
                                                  cacaTCGCGCTTTGCGAAGATGCCGCCAACCATATCGGGCACGTACTGCGCATGGGGCCGGGGCAGGCGTTGCAATTGTTTGACGGTAGCAAc                                       <  1:6882861/93‑1 (MQ=255)
                                                   acaTCGCGCTTTGCGAAGATGCCGCCAACCATATCGGGCACGTACTGCGCATGGGGCCGGGGCAGGCGTTGCAATTGTTTGACGGTAGCAAcc                                      <  1:7157722/93‑1 (MQ=255)
                                                     aTCGCGCTTTGCGAAGATGCCGCCAACCATATCGGGCACGTACTGCGCATGGGGCCGGGGCAGGCGTTGCAATTGTTTGACGGTAGCAACCAg                                    <  1:7686220/93‑1 (MQ=255)
                                                     aTCGCGCTTTGCGAAGATGCCGCCAACCATATCGGGCACGTACTGCGCATGGGGCCGGGGCAGGCGTTGCAATTGTTTGACGGTAGCAACCAg                                    <  2:6437408/93‑1 (MQ=255)
                                                      tCGCGCTTTGCGAAGATGCCGCCAACCATATCGGGCACGTACTGCGCATGGGGCCGGGGCAGGCGTTGCAATTGTTTGACGGTAGCAACCAgg                                   >  1:456643/1‑93 (MQ=255)
                                                      tCGCGCTTTGCGAAGATGCCGCCAACCATATCGGGCACGTACTGCGCATGAGGCCGGGGCAGGCGTTGCAATTGTTTGACGGCAGCAACCAgg                                   >  2:3702754/1‑93 (MQ=255)
                                                        gcgcTTTGCGAAGATGCCGCCAACCATATCGGGCACGTACTGCGCATGGGGCCGGGGCAGGCGTTGCAATTGTTTGACGGTAGCAACCAGGTc                                 >  2:3949847/1‑93 (MQ=255)
                                                        gcgcTTTGCGAAGATGCCGCCAACCATATCGGGCACGTACTGCGCATGGGGCCGGGGCAGGCGTTGCAATTGTTTGACGGTAGCAACCAGGTc                                 >  1:4184373/1‑93 (MQ=255)
                                                         cgcTTTGCGAAGATGCCGCCAACCATATCGGGCGCGTACTGCGCATGGGGCCGGGGCAGGCGTTGCAATTGTTTGACGGTAGCAACCAGGTCt                                >  1:6234567/1‑93 (MQ=255)
                                                         cgcTTTGCGAAGATGCCGCCAACCATATCGGGCACGTACTGCGCATGGGGCCGGGGCAGGCGTTGCAATTGTTTGACGGTAGCAACCAGGTCt                                <  2:1066723/93‑1 (MQ=255)
                                                          gcTTTGCGAAGATGCCGCCAACCATATCGGGCACGTACTGCGCATGGGGCCGGGGCAGGCGTTGCAATTGTTTGACGGTAGCAACCAGGTCtt                               <  2:136248/93‑1 (MQ=255)
                                                          gcTTTGCGAAGATGCCGCCAACCATATCGGGCACGTACTGCGCATGGGGCCGGGGCAGGCGTTGCAATTGTTTGACGGTAGCAACCAGGTCtt                               <  1:5469017/93‑1 (MQ=255)
                                                           cTTTGCGAAGATGCCGCCAACCATATCGGGCACGTACTGCGCATGGGGCCGGGGCAGGCGTTGCAATTGTTTGACGGTAGCAACCAGGTCttt                              <  1:4668742/93‑1 (MQ=255)
                                                            tttGCGAAGATGCCGCCAACCATATCGGGCACGTACTGCGCATGGGGCCGGGGCAGGCGTTGCAATTGTTTGACGGTAGCAACCAGGTCTTTg                             >  2:5306704/1‑93 (MQ=255)
                                                            tttGCGAAGATGCCGCCAACCATATCGGGCACGTACTGCGCATGGGGCCGGGGCAGGCGTTGCAATTGTTTGACGGTAGCAACCAGGTCTTTg                             >  1:3279666/1‑93 (MQ=255)
                                                             ttGCGAAGATGCCGCCAACCATATCGGGCACGTACTGCGCATGGGGCCGGGGCAGGCGTTGCAATTGTTTGACGTTAGCAACCAGGTCTTTGa                            <  2:7744606/93‑1 (MQ=255)
                                                             ttGCGAAGATGCCGCCAACCATATCGGGCACGTACTGCGCATGGGGCCGGGGCAGGCGTTGCAATTGTTTGACGGTAGCAACCAGGTCTTTGa                            >  2:1245126/1‑93 (MQ=255)
                                                                 gAAGATGCCGCCAACCATATCGGGCACGTACTGCGCATGGGGCCGGGGCAGGCGTTGCAATTGTTTGACGGTAGCAACCAGGTCTTTGACGcc                        >  1:3689380/1‑93 (MQ=255)
                                                                    gATGCCGCCAACCATATCGGGCACGTACTGCGCATGGGGCCGGGGCAGGCGTTGCAATTGTTTGACGGTAGCAACCAGGTCTTTGACGCCGaa                     >  1:2220321/1‑93 (MQ=255)
                                                                        ccgccAACCATATCGGGCACGTACTGCGCATGGGGCCGGGGCAGGCGTTGCAATTGTTTGACGGTAGCAACCAGGTCTTTGACGCCGAAATTa                 <  2:341780/93‑1 (MQ=255)
                                                                         cgccAACCATATCGGGCACGTATTGCGCATGGGGCCGGGGCAGGCGTTGCAATTGTTTGACGGTAGCAACCAGGTCTTTGACGCCGAAATTAc                >  2:4460409/1‑93 (MQ=255)
                                                                          gccAACCATATCGGGCACGTACTGCGCATGGGGCCGGGGCAGGCGTTGCAATTGTTTGACGGTAGCAACCAGGTCTTTGACGCCGAAATTAcc               <  1:5830383/93‑1 (MQ=255)
                                                                          gccAACCATATCGGGCACGTACTGCGCATGGGGCCGGGGCAGGCGTTGCAATTGTTTGACGGTAGCAACCAGGTCTTTGACGCCGAAATTAcc               <  1:6642261/93‑1 (MQ=255)
                                                                             aaCCATATCGGGCACGTACTGCGCATGGGGCCGGGGCAGGCGTTGCAATTGTTTGACGGTAGCAACCAGGTCTTTGACGCCGAAATTACCAgc            >  2:5369362/1‑93 (MQ=255)
                                                                              aCCATATCGGGCACGTACTGCGCATGGGGCCGGGGCAGGCGTTGCAATTGTTTGACGGTAGCAACCAGGTCTTTGACGCCGAAATTACCAgcg           <  2:7505649/93‑1 (MQ=255)
                                                                               ccATATCGGGCACGTACTGCGCATGGGGCCGGGGCAGGCGTTGCAATTGTTTGACGGTAGCAACCAGGTCTTTGACGCCGAAATTACCAgcgc          >  2:5625088/1‑93 (MQ=255)
                                                                                 atatCGGGCACGTACTGCGCATGGGGCCGGGGCAGGCGTTGCAATTGTTTGACGGTAGCAACCAGGTCTTTGACGCCGAAATTACCAGCGCCa        <  2:439657/93‑1 (MQ=255)
                                                                                 atatCGGGCACGTACTGCGCATGGGGCCGGGGCAGGCGTTGCAATTGTTTGACGGTAGCAACCAGGTCTTTGACGCCGAAATTACCAGCGCCa        <  2:7679148/93‑1 (MQ=255)
                                                                                 atatCGGGCACGTACTGCGCATGGGGCCGGGGCAGGCGTTGCAATTGTTTGACGGTAGCAACCAGGTCTTTGACGCCGAAATTACCAGCGCCa        >  2:7020091/1‑93 (MQ=255)
                                                                                   atCGGGCACGTACTGCGCATGGGGCCGGGGCAGGCGTTGCAATTGTTTGACGGTAGCAACCAGGTCTTTGACGCCGAAATTACCAGCGCCAGc      <  1:3128655/93‑1 (MQ=255)
                                                                                   atCGGGCACGTACTGCGCATGGGGCCGGGGCAGGCGTTGCAATTGTTTGACGGTAGCAACCAGGTCTTTGACGCCGAAATTACCAGCGCCAGc      >  1:6555354/1‑93 (MQ=255)
                                                                                    tCGGGCACGTACTGCGCATGGGGCCGGGGCAGGCGTTGCAATTGTTTGACGGTAGCAATCAGGTCTTTGACGCCGAAATTACCAGCGCCAGCa     >  1:4832915/1‑93 (MQ=255)
                                                                                      gggCACGTACTGCGCATGGGGCCGGGGCAGGCGTTGCAATTGTTTGACGGTAGCAACCAGGTCTTTGACGCCGAAATTACCAGCGCCACCaaa   >  2:1502048/1‑93 (MQ=255)
                                                                                          |                                                                                         
CTATGCGTATCCCCCGCATTTATCATCCTGAACCACTGACCAGCCATTCTCACATCGCGCTTTGCGAAGATGCCGCCAACCATATCGGGCGCGTACTGCGCATGGGGCCGGGGCAGGCGTTGCAATTGTTTGACGGTAGCAACCAGGTCTTTGACGCCGAAATTACCAGCGCCAGCAAAA  >  NC_000913/3089151‑3089330

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output Only 200 of 201 total aligned reads displayed.
TAACTATGCGTATCCCCCGCATTTATCATCCTGAACCACTGACCAGCCATTCTCACATCGCGCTTTGCGAAGATGCCGCCAACCATATCGGGCGCGTACTGCGCATGGGGCCGGGGCAGGCGTTGCAATTGTTTGACGGTAGCAACCAGGTCTTTGACGCCGAAATTACCAGCGCCAGCAAAAAAAG  >  NC_000913/3089148‑3089334
                                                                                             |                                                                                             
TAACTATGCGTATCCCCCGCATTTATCATCCTGAACCACTGACCAGCCATTCTCACATCGCGCTTTGCGAAGATGCCGCCAACCATATCGGGCA                                                                                               >  SRR4302543.7004336/1‑94 (MQ=60)
TAACTATGCGTATCCCCCGCATTTATCATCCTGAACCACTGACCAGCCATTCTCACATCGCGCTTTGCGAAGATGCCGCCAACCATATCGGGCA                                                                                               <  SRR4302543.7552638/94‑1 (MQ=60)
 AACTATGCGTATCCCCCGCATTTATCATCCTGAACCACTGCCCAGCCATTCTCACATCGCGCTTTGCGAAGATGCCGCCAACCATATCGGGCAC                                                                                              <  SRR4302543.1703537/94‑1 (MQ=60)
 AACTATGCGTATCCCCCGCATTTATCATCCTGAACCACTGACCAGCCATTCTCACATCGCGCTTTGCGAAGATGCCGCCAACCATATCGGGCAC                                                                                              <  SRR4302543.2963353/94‑1 (MQ=60)
 AACTATGCGTATCCCCCGCATTTATCATCCTGAACCACTGACCAGCCATTCTCACATCGCGCTTTGCGAAGATGCCGCCAACCATATCGGGCAC                                                                                              >  SRR4302543.527951/1‑94 (MQ=60)
 AACTATGCGTATCCCCCGCATTTATCATCCTGAACCACTGACCAGCCATTCTCACATCGCGCTTTGCGAAGATGCCGCCAACCATATCGGGCAC                                                                                              >  SRR4302543.527950/1‑94 (MQ=60)
 AACTATGCGTATCCCCCGCATTTATCATCCTGAACCACTGACCAGCCATTCTCACATCGCGCTTTGCGAAGATGCCGCCAACCATATCGGGCAC                                                                                              <  SRR4302543.7166508/94‑1 (MQ=60)
 AACTATGCGTATCCCCCGCATTTATCATCCTGAACCACTGACCAGCCATTCTCACATCGCGCTTTGCGAAGATGCCGCCAACCATATCGGGCAC                                                                                              <  SRR4302543.5166266/94‑1 (MQ=60)
 AACTATGCGTATCCCCCGCATTTATCATCCTGAACCACTGACCAGCCATTCTCACATCGCGCTTTGCGAAGATGCCGCCAACCATATCGGGCAC                                                                                              <  SRR4302543.7983350/94‑1 (MQ=60)
 AACTATGCGTATCCCCCGCATTTATCATCCTGAACCACTGACCAGCCATTCTCACATCGCGCTTTGCGAAGATGCCGCCAACCATATCGGGCAC                                                                                              <  SRR4302543.2103472/94‑1 (MQ=60)
   CTATGCGTATCCCCCGCATTTATCATCCTGAACCACTGACCAGCCATTCTCACATCGCGCTTTGCGAAGATGCCGCCAACCATATCGGGCACGT                                                                                            >  SRR4302543.780605/1‑94 (MQ=60)
     ATGCGTATCCCCCGCATTTATCATCCTGAACCACTGACCAGCCATTCTCACATCGCGCTTTGCGAAGATGCCGCCAACCATATCGGGCACGTAC                                                                                          >  SRR4302543.174288/1‑94 (MQ=60)
      TGCGTATCCCCCGCATTTATCATCCTGAACCACTGACCAGCCATTCTCACATCGCGCTTTGCGAAGATGCCGCCAACCATATCGGGCACGTACT                                                                                         <  SRR4302543.2448592/94‑1 (MQ=60)
      TGCGTATCCCCCGCATTTATCATCCTGAACCACTGACCAGCCATTCTCACATCGCGCTTTGCGAAGATGCCGCCAACCATATCGGGCACGTACT                                                                                         <  SRR4302543.1920420/94‑1 (MQ=60)
      TGCGTATCCCCCGCATTTATCATCCTGAACCACTGACCAGCCATTCTCACATCGCGCTTTGCGAAGATGCCGCCAACCATATCGGGCACGTACT                                                                                         <  SRR4302543.242869/94‑1 (MQ=60)
      TGCGTAGCCCCCGCATTTATCATCCTGAACCACTGTCCAGCCATTCTCACATCGCGCTTTGCGAAGATGCCGCCAACCATATCGGGCACGTACT                                                                                         <  SRR4302543.308972/94‑1 (MQ=60)
       GCGTATCCCCCGCATTTATCATCCTGAACCACTGACCAGCCATTCTCACATCGCGCTTTGCGAAGATGCCGCCAACCATATCGGGCACGTACTG                                                                                        <  SRR4302543.7880180/94‑1 (MQ=60)
       GCGTATCCCCCGCATTTATCATCCTGAACCACTGACCAGCCATTCTCACATCGCGCTTTGCGAAGATGCCGCCAACCATATCGGGCACGTACTG                                                                                        <  SRR4302543.1257158/94‑1 (MQ=60)
       GCGTATCCCCCGCATTTATCATCCTGAACCACTGACCAGCCATTCTCACATCGCGCTTTGCGAAGATGCCGCCAACCATATCGGGCACGTACTG                                                                                        >  SRR4302543.1149908/1‑94 (MQ=60)
       GCGTATCCCCCGCATTTATCATCCTGAACCACTGACCAGCCATTCTCACATCGCGCTTTGCGAAGATGCCGCCAACCATATCGGGCACGTACTG                                                                                        <  SRR4302543.1055061/94‑1 (MQ=60)
        CGTATCCCCCGCATTTATCATCCTGAACCACTGACCAGCCATTCTCACATCGCGCTTTGCGAAGATGCCGCCAACCATATCGGGCACGTACTGC                                                                                       >  SRR4302543.4594363/1‑94 (MQ=60)
        CGTATCCCCCGCATTTATCATCCTGAACCACTGACCAGCCATTCTCACATCGCGCTTTGCGAAGATGCCGCCAACCATATCGGGCACGTACTGC                                                                                       >  SRR4302543.4094075/1‑94 (MQ=60)
         GTATCCCCCGCATTTATCATCCTGAACCACTGACCAGCCATTCTCACATCGCGCTTTGCGAAGATGCCGCCAACCATATCGGGCACGTACTGCG                                                                                      <  SRR4302543.6955678/94‑1 (MQ=60)
         GTATCCCCCGCATGTATCATCCTGAACCACTGACCAGCCATTCTCACATCGCGCTTTGCGAAGATGCCGCCAACCATATCGGGCACGTACTGCG                                                                                      <  SRR4302543.1144731/94‑1 (MQ=60)
          TATCCCCCGCATTTATCATCCTGAACCACTGACCAGCCATTCTCACATCGCGCTTTGCGAAGATGCCGCCAACCATATCGGGCACGTACTGCGC                                                                                     >  SRR4302543.7662999/1‑94 (MQ=60)
           ATCCCCCGCATTTATCATCCTGAACCACTGACCAGCCATTCTCACATCGCGCTTTGCGAAGATGCCGCCAACCATATCGGGCACGTACTGCGCA                                                                                    <  SRR4302543.3649870/94‑1 (MQ=60)
           ATCCCCCGCATTTATCATCCTGAACCACTGACCAGCCATTCTCACATCGCGCTTTGCGAAGATGCCGCCAACCATATCGGGCACGTACTGCGCA                                                                                    <  SRR4302543.6629729/94‑1 (MQ=60)
             CCCCCGCATTTATCATCCTGAACCACTGCCCAGCCATTCTCACATCGCGCTTTGCGAAGATGCCGCCAACCATATCGGGCACGTACTGCGCATG                                                                                  <  SRR4302543.190699/94‑1 (MQ=60)
             CCCCCGCATTTATCATCCTGAACCACTGACCAGCCATTCTCACATCGCGCTTTGCGAAGATGCCGCCAACCATATCGGGCACGTACTGCGCATG                                                                                  <  SRR4302543.6588418/94‑1 (MQ=60)
             CCCCCGCATTTATCATCCTGAACCACTGACCAGCCATTCTCACATCGCGCTTTGCGAAGATGCCGCCAACCATATCGGGCACGTACTGCGCATG                                                                                  >  SRR4302543.2424493/1‑94 (MQ=60)
              CCCCGCATTTATCATCCTGAACCAGTGACCAGCCATTCTCACATCGCGCTTTGCGAAGATGCCGCCAACCATATCGGGCACGTACTGCGCATGG                                                                                 <  SRR4302543.3597828/94‑1 (MQ=60)
              CCCCGCATTTATCATCCTGAACCACTGACCAGCCATTCTCACATCGCGCTTTGCGAAGATGCCGCCAACCATATCGGGCACGTACTGCGCATGG                                                                                 <  SRR4302543.3345198/94‑1 (MQ=60)
              CCCCGCATTTATCATCCTGAACCACTGACCAGCCATTCTCACATCGCGCTTTGCGAAGATGCCGCCAACCATATCGGGCACGTACTGCGCATGG                                                                                 >  SRR4302543.7428097/1‑94 (MQ=60)
              CCCCGCATTTATCATCCTGAACCACTGACCAGCCATTCTCACATCGCGCTTTGCGAAGATGCCGCCAACCATATCGGGCACGTACTGCGCATGG                                                                                 <  SRR4302543.3597829/94‑1 (MQ=60)
              CCCCGCATTTATCATCCTGAACCACTGACCAGCCATTCTCACATCGCGCTTTGCGAAGATGCCGCCAACCATATCGGGCACGTACTGCGCATGG                                                                                 <  SRR4302543.1047954/94‑1 (MQ=60)
              CCCCGCATTTATCATCCTGAACCACTGACCAGCCATTCTCACATCGCGCTTTGCGAAGATGCCGCCAACCATATCGGGCACGTACTGCGCATGG                                                                                 >  SRR4302543.2010888/1‑94 (MQ=60)
              CCCCGCATTTATCATCCTGAACCACTGACCAGCCATTCTCACATCGCGCTTTGCGAAGATGCCGCCAACCATATCGGGCACGTACTGCGCATGG                                                                                 <  SRR4302543.1047955/94‑1 (MQ=60)
               CCCGCATTTATCATCCTGACCCACTGACCAGCCATTCTCACATCGCGCTTTGCGAAGATGCCGCCAACCATATCGGGCACGTACTGCGCATGGG                                                                                <  SRR4302543.1453350/94‑1 (MQ=60)
               CCCGCATTTATCATCCTGAACCACTGACCAGCCATTCTCACATCGCGCTTTGCGAAGATGCCGCCAACCATATCGGGCACGTACTGCGCATGGG                                                                                >  SRR4302543.2705568/1‑94 (MQ=60)
               CCCGCATTTATCATCCTGAACCACTGACCAGCCATTCTCACATCGCGCTTTGCGAAGATGCCGCCAACCATATCGGGCACGTACTGCGCATGGG                                                                                <  SRR4302543.7912592/94‑1 (MQ=60)
               CCCGCATTTATCATCCTGAACCACTGACCAGCCATTCTCACATCGCGCTTTGCGAAGATGCCGCCAACCATATCGGGCACGTACTGCGCATGGG                                                                                <  SRR4302543.2477759/94‑1 (MQ=60)
                CCGCATTTATCATCCTGAACCACTGACCAGCCATTCTCACATCGCGCTTTGCGAAGATGCCGCCAACCATATCGGGCACGTACTGCGCATGGGG                                                                               <  SRR4302543.1886407/94‑1 (MQ=60)
                CCGCATTTATCATCCTGAACCACTGACCAGCCATTCTCACATCGCGCTTTGCGAAGATGCCGCCAACCATATCGGGCACGTACTGCGCATGGGG                                                                               >  SRR4302543.6571613/1‑94 (MQ=60)
                 CGCATTTATCATCCTGAACCACTGACCAGCCATTCTCACATCGCGCTTTGCGAAGATGCCGCCAACCATATCGGGCACGTACTGCGCATGGGGC                                                                              <  SRR4302543.7621004/94‑1 (MQ=60)
                  GCATTTATCATCCTGAACCACTGACCAGCCATTCTCACATCGCGCTTTGCGAAGATGCCGCCAACCATATCGGGCGCGTACTGCGCATGGGGCC                                                                             >  SRR4302543.8002945/1‑94 (MQ=60)
                  CCATTTATCATCCTGAACCACTGACCAGCCATTCTCACATCGCGCTTTGCGAAGATGCCGCCAACCATATCGGGCACGTACTGCGCATGGGGCC                                                                             <  SRR4302543.2066641/94‑1 (MQ=60)
                    ATTTATCATCCTGAACCACTGACCAGCCATTCTCACATCGCGCTTTGCGAAGATGCCGCCAACCATATCGGGCACGTACTGCGCATGGGGCCGG                                                                           <  SRR4302543.2127791/94‑1 (MQ=60)
                    ATTTATCATCCTGAACCACTGACCAGCCATTCTCACATCGCGCTTTGCGAAGATGCCGCCAACCATATCGGGCACGTACTGCGCATGGGGCCGG                                                                           >  SRR4302543.2103528/1‑94 (MQ=60)
                    ATTTATCATCCTGAACCACTGACCAGCCATTCTCACATCGCGCTTTGCGAAGATGCCGCCAACCATATCGGGCACGTACTGCGCATGGGGCCGG                                                                           >  SRR4302543.2092778/1‑94 (MQ=60)
                    ATTTATCATCCTGAACCACTGACCAGCCATTCTCACATCGCGCTTTGCGAAGATGCCGCCAACCATATCGGGCACGTACTGCGCATGGGGCCGG                                                                           <  SRR4302543.1406220/94‑1 (MQ=60)
                    ATTTATCATCCTGAACCACTGACCAGCCATTCTCACATCGCGCTTTGCGAAGATGCCGCCAACCATATCGGGCACGTACTGCGCATGGGGCCGG                                                                           >  SRR4302543.7386702/1‑94 (MQ=60)
                    ATTTATCATCCTGAACCACTGACCAGCCATTCTCACATCGCGCTTTGCGAAGATGCCGCCAACCATATCGGGCACGTACTGCGCATGGGGCCGG                                                                           >  SRR4302543.2679405/1‑94 (MQ=60)
                    ATTTATCATCCTGAACCACTGACCAGCCATTCTCACATCGCGCTTTGCGAAGATGCCGCCAACCATATCGGGCACGTACTGCGCATGGGGCCGG                                                                           >  SRR4302543.2679404/1‑94 (MQ=60)
                    ATTTATCATCCTGAACCACTGACCAGCCATTCTCACATCGCGCTTTGCGAAGATGCCGCCAACCATATCGGGCACGTACTGCGCATGGCGCCGG                                                                           >  SRR4302543.3094097/1‑94 (MQ=60)
                     TTTATCATCCTGAACCACTGACCAGCCATTCTCACATCGCGCTTTGCGAAGATGCCGCCAACCATATCGGGCACGTACTGCGCATGGGGCCGGG                                                                          <  SRR4302543.3522299/94‑1 (MQ=60)
                     TTTATCATCCTGAACCACTGACCAGCCATTCTCACATCGCGCTTTGCGAAGATGCCGCCAACCATATCGGGCACGTACTGCGCATGGGGCCGGG                                                                          >  SRR4302543.4186942/1‑94 (MQ=60)
                     TTTATCATCCTGAACCACTGACCAGCCATTCTCACATCGCGCTTTGCGAAGATGCCGCCAACCATATCGGGCACGTACTGCGCATGGGGCCGGG                                                                          <  SRR4302543.4234769/94‑1 (MQ=60)
                     TTTATCATCCTGAACCACTGACCAGCCATTCTCACATCGCGCTTTGCGAAGATGCCGCCAACCATATCGGGCACGTACTGCGCATGGGGCCGGG                                                                          <  SRR4302543.2479530/94‑1 (MQ=60)
                     TTTATCATCCTGAACCACTGACCAGCCATTCTCACATCGCGCTTTGCGAAGATGCCGCCAACCATATCGGGCACGTACTGCGCATGGGGCCGGC                                                                          >  SRR4302543.5247443/1‑94 (MQ=60)
                     TGTATCATCCTGAACCACTGACCAGCCATTCTCACATCGCGCTTTGCGAAGATGCCGCCAACCATATCGGGCACGTACTGCGCATGGGGCCGGG                                                                          <  SRR4302543.3890038/94‑1 (MQ=60)
                      TTATCATCCTGAACCACTGACCAGCCATTCTCACATCGCGCTTTGCGAAGATGCCGCCAACCATATCGGGCACGTACTGCGCATGGGGCCGGGG                                                                         >  SRR4302543.1787685/1‑94 (MQ=60)
                       TATCATCCTGAACCACTGACCAGCCATTCTCACATCGCGCTTTGCGAAGATGCCGCCAACCATATCGGGCACGTACTGCGCATGGGGCCGGGGC                                                                        <  SRR4302543.5622725/94‑1 (MQ=60)
                       TATCATCCTGAACCACTGACCAGCCATTCTCACATCGCGCTTTGCGAAGATGCCGCCAACCATATCGGGCACGTACTGCGCATGGGGCCGGGGC                                                                        <  SRR4302543.5021121/94‑1 (MQ=60)
                        ATCATCCTGAACCACTGACCAGCCATTCTCACATCGCGCTTTGCGAAGATGCCGCCAACCATATCGGGCACGTACTGCGCATGGGGCCGGGGCA                                                                       <  SRR4302543.5845326/94‑1 (MQ=60)
                        ATCATCCTGAACCACTGACCAGCCATTCTCACATCGCGCTTTGCGAAGATGCCGCCAACCATATCGGGCACGTACTGCGCATGGGGCCGGGGCA                                                                       >  SRR4302543.2565322/1‑94 (MQ=60)
                          CATCCTGAACCACTGACCAGCCATTCTCACATCGCGCTTTGCGAAGATGCCGCCAACCATATCGGGCACGTACTGCGCATGGGGCCGGGGCAGG                                                                     <  SRR4302543.6217643/94‑1 (MQ=60)
                           ATCCTGAACCACTGACCAGCCATTCTCACATCGCGCTTTGCGAAGATGCCGCCAACCATATCGGTCGCGTACTGCGCATGGGGCCGGGGCAGGC                                                                    >  SRR4302543.2604445/1‑94 (MQ=60)
                           ATCCTGAACCACTGACCAGCCATTCTCACATCGCGCTTTGCGAAGATGCCGCCAACCATATCGGGCACCTACTGCGCATGGGGCCGGGGCAGGC                                                                    >  SRR4302543.220488/1‑94 (MQ=60)
                            TCCTGAACCACTGACCAGCCATTCTCACATCGCGCTTTGCGAAGATGCCGCCAACCATATCGGGCACGTACTGCGCATGGGGCCGGGGCAGGCG                                                                   <  SRR4302543.498882/94‑1 (MQ=60)
                             CCTGAACCACTGACCAGCCATTCTCACATCGCGCTTTGCGAAGATGCCGCCAACCATATCGGGCACGTACTGCGCATGGGGCCGGGGCAGGCGT                                                                  >  SRR4302543.1193647/1‑94 (MQ=60)
                             CCTGAACCACTGACCAGCCATTCTCACATCGCGCTTTGCGAAGATGCCGCCAACCATATCGGGCACGTACTGCGCATGGGGCCGGGGCAGGCGT                                                                  >  SRR4302543.7041424/1‑94 (MQ=60)
                              CTGAACCACTGACCAGCCATTCTCACATCGCGCTTTGCGAAGATGCCGCCAACCATATCGGGCACGTACTGCGCATGGGGCCGGGGCAGGCGTT                                                                 >  SRR4302543.7170051/1‑94 (MQ=60)
                              CTGAACCACTGACCAGCCATTCTCACATCGCGCTTTGCGAAGATGCCGCCAACCATATCGGGCACGTACTGCGCATGGGGCCGGGGCAGGCGTT                                                                 >  SRR4302543.6661488/1‑94 (MQ=60)
                              CTGAACCACTGACCAGCCATTCTCACATCGCGCTTTGCGAAGATGCCGCCAACCATATCGGGCACGTACTGCGCATGGGGCCGGGGCAGGCGTT                                                                 >  SRR4302543.7170050/1‑94 (MQ=60)
                              CTGAACCACTGACCAGCCATTCTCACATCGCGCTTTGCGAAGATGCCGCCAACCATATCGGGCACGTACTGCGCATGGGGCCGGGGCAGGCGTT                                                                 <  SRR4302543.1344344/94‑1 (MQ=60)
                              CTGAACCACTGACCAGCCATTCTCACATCGCGCTTTGCGAAGATGCCGCCAACCATATCGGGCACGTACTGCGCATGGGGCCGGGGCAGGCGTT                                                                 <  SRR4302543.1344343/94‑1 (MQ=60)
                              CTGAACCACTGACCAGCCATTCTCACATCGCGCTTTGCGAAGATGCCGCCAACCATATCGGGCACGTACTGCGCATGGGGCCGGGGCAGGCGTA                                                                 >  SRR4302543.1849102/1‑94 (MQ=60)
                               TGAACCACTGACCAGCCATTCTCACATCGCGCTTTGCGAAGATGCCGCCAACCATATCGGGCACGTACTGCGCATGGGGCCGGGGCAGGCGTTG                                                                >  SRR4302543.4782084/1‑94 (MQ=60)
                               TGAACCACTGACCAGCCATTCTCACATCGCGCTTTGCGAAGATGCCGCCAACCATATCGGGCACGTACTGCGCATGGGGCCGGGGCAGGCGTTG                                                                >  SRR4302543.1539182/1‑94 (MQ=60)
                               TGAACCACTGACCAGCCATTCTCACATCGCGCTTTGCGAAGATGCCGCCAACCATATCGGGCACGTACTGCGCATGGGGCCGGGGCAGGCGTTG                                                                >  SRR4302543.7640196/1‑94 (MQ=60)
                                GAACCACGGACCAGCCATTCTCACATCGCGCTTTGCGAAGATGCCGCCAACCATATCGGGCACGTACTGCGCATGGGGCCGGGGCAGGCGTTGC                                                               <  SRR4302543.2765479/94‑1 (MQ=60)
                                 AACCACTGACCAGCCATTCTCACATCGCGCTTTGCGAAGATGCCGCCAACCATATCGGGCACGTACTGCGCATGGGGCCGGGGCAGGCGTTGCA                                                              <  SRR4302543.5166186/94‑1 (MQ=60)
                                 AACCACTGACCAGCCATTCTCACATCGCGCTTTGCGAAGATGCCGCCAACCATATCGGGCACGTACTGCGCATGGGGCCGGGGCAGGCGTTGCA                                                              <  SRR4302543.1150522/94‑1 (MQ=60)
                                 AACCACTGACCAGCCATTCTCACATCGCGCTTTGCGAAGATGCCGCCAACCATATCGGGCACGTACTGCGCATGGGGCCGGGGCAGGCGTTGCA                                                              >  SRR4302543.5792863/1‑94 (MQ=60)
                                  ACCACTGACCAGCCATTCTCACATCGCGCTTTGCGAAGATGCCGCCAACCATATCGGGCACGTACTGCGCATGGGGCCGGGGCAGGCGTTGCAA                                                             >  SRR4302543.4657375/1‑94 (MQ=60)
                                  ACCACTGACCAGCCATTCTCACATCGCGCTTTGCGAAGATGCCGCCAACCATATCGGGCACGTACTGCGCATGGGGCCGGGGCAGGCGTTGCAA                                                             >  SRR4302543.5449905/1‑94 (MQ=60)
                                  ACCACTGACCAGCCATTCTCACATCGCGCTTTGCGAAGATGCCGCCAACCATATCGGGCACGTACTGCGCATGGGGCCGGGGCAGGCGTTGCAA                                                             >  SRR4302543.5925778/1‑94 (MQ=60)
                                  ACCACTGACCAGCCATTCTCACATCGCGCTTTGCGAAGATGCCGCCAACCATATCGGGCACGTACTGCGCATGGGGCCGGGGCAGGCGTTGCAA                                                             >  SRR4302543.6540651/1‑94 (MQ=60)
                                   CCACTGACCAGCCATTCTCACATCGCGCTTTGCGAAGATGCCGCCAACCATATCGGGCACGTACTGCGCATGGGGCCGGGGCAGGCGTTGCAAT                                                            >  SRR4302543.312778/1‑94 (MQ=60)
                                   CCACTGACCAGCCATTCTCACATCGCGCTTTGCGAAGATGCCGCCAACCATATCGGGCACGTACTGCGCATGGGGCCGGGGCAGGCGTTGCAAT                                                            >  SRR4302543.312780/1‑94 (MQ=60)
                                    CACTGCCCAGCCATTCTCACATCGCGCTTTGCGAAGATGCCGCAAACCATATCGGGCACGTACTGCGCATGGGGCCGGGGCAGGCGTTGCAATT                                                           <  SRR4302543.7660901/94‑1 (MQ=60)
                                    CACTGACCAGCCATTCTCACATCGCGCTTTGCGAAGATGCCGCCAACCATATCGGGCACGTACTGCGCATGGGGCCGGGGCAGGCGTTGCAATT                                                           <  SRR4302543.4440324/94‑1 (MQ=60)
                                     CCTGACCAGCCATTCTCACATCGCGCTTTGCGAAGATGCCGCCAACCATATCGGGCACGTACTGCGCATGGGGCCGGGGCAGGCGTTGCAATTG                                                          <  SRR4302543.6655093/94‑1 (MQ=60)
                                     CCTGACCAGCCATTCTCACATCGCGCTTTGCGAAGATGCCGCCAACCATATCGGGCACGTACTGCGCATGGGGCCGGGGCAGGCGTTGCAATTG                                                          <  SRR4302543.2706911/94‑1 (MQ=60)
                                      CTGACCAGCCATTCTCACATCGCGCTTTGCGAAGATGCCGCCAACCATATCGGGCACGTACTGCGCATGGGGCCGGGGCAGGCGTTGCAATTGT                                                         >  SRR4302543.6567785/1‑94 (MQ=60)
                                      CTGACCAGCCATTCTCACATCGCGCTTTGCGAAGATGCCGCCAACCATATCGGGCACGTACTGCGCATGGGGCCGGGGCAGGCGTTGCAATTGT                                                         <  SRR4302543.4322057/94‑1 (MQ=60)
                                      CTGACCAGCCATTCTCACATCGCGCTTTGCGAAGATGCCGCCAACCATATCGGGCACGTACTGCGCATGGGGCCGGGGCAGGCGTTGCAATTGT                                                         >  SRR4302543.5803803/1‑94 (MQ=60)
                                       TGACCAGCCATTCTCACATCGCGCTTTGCGAAGATGCCGCCAACCATATCGGGCACGTACTGCGCATGGGGCCGGGGCAGGCGTTGCAATTGTT                                                        <  SRR4302543.5409651/94‑1 (MQ=60)
                                         ACCAGCCATTCTCACATCGCGCTTTGCGAAGATGCCGCCAACCATATCGGGCACGTACTGTGCATGGGGCCGGGGCAGGCGTTGCAATTGTTTG                                                      >  SRR4302543.3225021/1‑94 (MQ=60)
                                          CCAGCCATTCTCACATCGCGCTTTGCGAAGATGCCGCCAACCATATCGGGCACGTACTGCGCATGGGGCCGGGGCAGGCGTTGCAATTGTTTGA                                                     <  SRR4302543.1712504/94‑1 (MQ=60)
                                           CAGCCATTCTCACATCGCGCTTTGCGAAGATGCCGCCAACCATATCGGGCACGTACTGCGCATGGGGCCGGGGCAGGCGTTGCAATTGTTTGAC                                                    <  SRR4302543.5760190/94‑1 (MQ=60)
                                           CAGCCATTCTCACATCGCGCTTTGCGAAGATGCCGCCAACCATATCGGGCACGTACTGCGCATGGGGCCGGGGCAGGCGTTGCAATTGTTTGAC                                                    >  SRR4302543.7175934/1‑94 (MQ=60)
                                           CAGCCATTCTCACATCGCGCTTTGCGAAGATGCCGCCAACCATATCGGGCACGTACTGCGCATGGGGCCGGGGCAGGCGTTGCAATTGTTTGAC                                                    >  SRR4302543.191795/1‑94 (MQ=60)
                                           CAGCCATTCTCACATCGCGCTTTGCGAAGATGCCGCCAACCATATCGGGCACGTACTGCGCATGGGGCCGGGGCAGGCGTTGCAATTGTTTGAC                                                    >  SRR4302543.2895377/1‑94 (MQ=60)
                                           CAGCCATTCTCACATCGCGCTTTGCGAAGATGCCGCCAACCATATCGGGCACGTACTGCGCATGGGGCCGGGGCAGGCGTTGCAATTGTTTGAC                                                    <  SRR4302543.3661774/94‑1 (MQ=60)
                                            AGCCATTCTCACATCGCGCTTTGCGAAGATGCCGCCAACCATATCGGGGACGTACTGCGCATGGGGCCGGGGCAGGCGTTGCAATTGTTTGACG                                                   >  SRR4302543.5922956/1‑94 (MQ=60)
                                            AGCCATTCTCACATCGCGCTTTGCGAAGATGCCGCCAACCATATCGGGCACGTACTGCGCATGGGGCCGGGGCAGGCGTTGCAATTGTTTGACG                                                   <  SRR4302543.4400991/94‑1 (MQ=60)
                                            AGCCATTCTCACATCGCGCTTTGCGAAGATGCCGCCAACCATATCGGGCACGTACTGCGCATGGGGCCGGGGCAGGCGTTGCAATTGTTTGACG                                                   <  SRR4302543.5640315/94‑1 (MQ=60)
                                            AGCCATTCTCACATCGCGCTTTGCGAAGATGCCGCCAACCATATCGGGCACGTACTGCGCATGGGGCCGGGGCAGGCGTTGCAATTGTTTGACG                                                   <  SRR4302543.1152453/94‑1 (MQ=60)
                                            AGCCATTCTCACATCGCGCTTTGCGAAGATGCCGCCAACCATATCGGGCACGTACTGCGCATGGGGCCGGGGCAGGCGTTGCAATTGTTTGACG                                                   >  SRR4302543.5922957/1‑94 (MQ=60)
                                              CCATTCTCACATCGCGCTTTGCGAAGATGCCGCCAACCATATCGGGCACGTACTGCGCATGGGGCCGGGGCAGGCGTTGCAATTGTTTGACGGT                                                 <  SRR4302543.737585/94‑1 (MQ=60)
                                              CCATTCTCACATCGCGCTTTGCGAAGATGCCGCCAACCATATCGGGCACGTACTGCGCATGGGGCCGGGGCAGGCGTTGCAATTGTTTGACGGT                                                 >  SRR4302543.1932591/1‑94 (MQ=60)
                                               CATTCTCACATCGCGCTTTGCGAAGATGCCGCCAACCATATCGGGCACGTACTGCGCATGGGGCCGGGGCAGGCGTTGCAATTGTTTGACGGTG                                                >  SRR4302543.5174974/1‑94 (MQ=60)
                                               CATTCTCACATCGCGCTTTGCGAAGATGCCGCCAACCATATCGGGCACGTACTGCGCATGGGGCCGGGGCAGGCGTTGCAATTGTTTGACGGTA                                                <  SRR4302543.4636784/94‑1 (MQ=60)
                                               CATTCTCACATCGCGCTTTGCGAAGATGCCGCCAACCATATCGGGCACGTACCGCGCATGGGGCCGGGGCAGGCGTTGCAATTGTTTGACGGTA                                                >  SRR4302543.4993719/1‑94 (MQ=60)
                                                ATTCTCACATCGCGCTTTGCGAAGATGCCGCCAACCATATCGGGCACGTACTGCGCATGGGGCCGGGGCAGGCGTTGCAATTGTTTGACGGTAG                                               >  SRR4302543.3200983/1‑94 (MQ=60)
                                                ATTCTCACATCGCGCTTTGCGAAGATGCCGCCAACCATATCGGGCACGTACTGCGCATGGGGCCGGGGCAGGCGTTGCAATTGTTTGACGGTAG                                               >  SRR4302543.4898895/1‑94 (MQ=60)
                                                ATTCTCACATCGCGCTTTGCGAAGATGCCGCCAACCATATCGGGCACGTACTGCGCATGGGGCCGGGGCAGGCGTTGCAATTGTTTGACGGTAG                                               >  SRR4302543.3484110/1‑94 (MQ=60)
                                                ATTCTCACATCGCGCTTTGCGAAGATGCCGCCAACCATATCGGGCACGTACTGCGCATGGGGCCGGGGCAGGCGTTGCAATTGTTTGACGGTAG                                               <  SRR4302543.5062321/94‑1 (MQ=60)
                                                ATTCTCACATCGCGCTTTGCGAAGATGCCGCCAACCATATCGGGCACGTACTGCGCATGGGGCCGGGGCAGGCGTTGCAATTGTTTGACGGTAG                                               >  SRR4302543.1916426/1‑94 (MQ=60)
                                                ATTCTCACATCGCGCTTTGCGAAGATGCCGCCAACCATATCGGGCACGTACTGCGCATGGGGCCGGGGCAGGCGTTGAAATTGTTTGACGGTAG                                               >  SRR4302543.2223818/1‑94 (MQ=60)
                                                ATTCTCACATCGCGCTTTGCGAAGATGCAGCCAACCATATCGGGCACGTACTGCGCATGGGGCAGGGGCAGGCGTTGCAATTGTTTGACGGTAG                                               <  SRR4302543.3621336/94‑1 (MQ=60)
                                                 TTCTCACATCGCGCTTTGCGAAGATGCCGCCAACCATATCGGGCACGTACTGCGCATGGGGCCGGGGCAGGCGTTGCAATTGTTTGACGGTAGC                                              <  SRR4302543.178064/94‑1 (MQ=60)
                                                   CTCACATCGCGCTTTGCGAAGATGCCGCCAACCATATCGGGCACGTACTGCGCATGGGGCCGGGGCAGGCGTTGCAATTGTTTGACGGTAGCAA                                            >  SRR4302543.326836/1‑94 (MQ=60)
                                                     CACATCGCGCTTTGCGAAGATGCCGCCAACCATATCGGGCACGTACTGCGCATGGGGCCGGGGCAGGCGTTGCAATTGTTTGACGGTAGCAACC                                          <  SRR4302543.6947616/94‑1 (MQ=60)
                                                      ACATCGCGCTTTGCGAAGATGCCGCCAACCATATCGGGCACGTACTGCGCATGGGGCCGGGGCAGGCGTTGCAATTGTTTGACGGTAGCAACCA                                         >  SRR4302543.879260/1‑94 (MQ=60)
                                                      ACATCGCGCTTTGCGAAGATGCCGCCAACCATATCGGGCACGTACTGCGCATGGGGCCGGGGCAGGCGTTGCAATTGTTTGACGGTAGCAACCA                                         <  SRR4302543.7227281/94‑1 (MQ=60)
                                                      ACATCGCGCTTTGCGAAGATGCCGCCAACCATATCGGGCACGTACTGCGCATGGGGCCGGGGCAGGCGTTGCAATTGTTTGACGGTAGCAACCA                                         <  SRR4302543.568764/94‑1 (MQ=60)
                                                      ACATCGCGCTTTGCGAAGATGCCGCCAACCATATCGGGCACGTACTGCGCATGGGGCCGGGGCAGGCGTTGCAATTGTTTGACGGTAGCAACCA                                         >  SRR4302543.4816597/1‑94 (MQ=60)
                                                       CATCGCGCTTTGCGAAGATGCCGCCAACCATATCGGGCACGTACTGCGCATGGGGCCGGGGCAGGCGTTGCAATTGTTTGACGGTAGCACCCAG                                        >  SRR4302543.5892083/1‑94 (MQ=60)
                                                       CATCGCGCTTTGCGAAGATGCCGCCAACCATATCGGGCACGTACTGCGCATGGGGCCGGGGCAGGCGTTGCAATTGTTTGACGGTAGCAACCAG                                        >  SRR4302543.2524405/1‑94 (MQ=60)
                                                       CATCGCGCTTTGCGAAGATGCCGCCAACCATATCGGGCACGTACTGCGCATGGGGCCGGGGCAGGCGTTGCAATTGTTTGACGGTAGCAACCAG                                        >  SRR4302543.1396586/1‑94 (MQ=60)
                                                       CATCGCGCTTTGCGAAGATGCCGCCAACCATATCGGGCACGTACTGCGCATGGGGCCGGGGCAGGCGTTGCAATTGTTTGACGGTAGCAACCAG                                        >  SRR4302543.4438248/1‑94 (MQ=60)
                                                        ATCGCGCTTTGCGAAGATGCCGCCAACCATATCGGGCACGTACTGCGCATGGGGCCGGGGCAGGCGTTGCAATTGTTTGACGGTAGCAACCAGG                                       <  SRR4302543.7759011/94‑1 (MQ=60)
                                                        ATCGCGCTTTGCGAAGATGCCGCCAACCATATCGGGCACGTACTGCGCATGGGGCCGGGGCAGGCGTTGCAATTGTTTGACGGTAGCAACCAGG                                       >  SRR4302543.460893/1‑94 (MQ=60)
                                                          CGCGCTTTGCGAAGATGCCGCCAACCATATCGGGCACGTACTGCGCATGGGGCCGGGGCAGGCGTTGCAATTGTTTGACGGTAGCAACCAGGTC                                     >  SRR4302543.4222297/1‑94 (MQ=60)
                                                          CGCGCTTTGCGAAGATGCCGCCAACCATATCGGGCACGTACTGCGCATGGGGCCGGGGCAGGCGTTGCAATTGTTTGACGGTAGCAACCAGGTC                                     <  SRR4302543.3350800/94‑1 (MQ=60)
                                                           GCGCTTTGCGAAGATGCCGCCAACCATATCGGGCGCGTACTGCGCATGGGGCCGGGGCAGGCGTTGCAATTGTTTGACGGTAGCAACCAGGTCT                                    >  SRR4302543.6293409/1‑94 (MQ=60)
                                                           GCGCTTTGCGAAGATGCCGCCAACCATATCGGGCACGTACTGCGCATGGGGCCGGGGCAGGCGTTGCAATTGTTTGACGGTAGCAACCAGGTCT                                    >  SRR4302543.3823221/1‑94 (MQ=60)
                                                           GCGCTTTGCGAAGATGCCGCCAACCATAGCGGGCACGTACTGCGCATGGGGCCGGGGCAGGCGTTGCAATTGTTTGACGGTAGCAACCAGGTCT                                    <  SRR4302543.1266512/94‑1 (MQ=60)
                                                            CGCTTTGCGAAGATGCCGCCAACCATATCGGGCACGTACTGCGCATGGGGCCGGGGCAGGCGTTGCAATTGTTTGACGGTAGCAACCAGGTCTT                                   >  SRR4302543.5011460/1‑94 (MQ=60)
                                                            CGCTTTGCGAAGATGCCGCCAACCATATCGGGCACGTACTGCGCATGGGGCCGGGGCAGGCGTTGCAATTGTTTGACGGTAGCAACCAGGTCTT                                   >  SRR4302543.4210858/1‑94 (MQ=60)
                                                             GCTTTGCGAAGATGCCGCCAACCATATCGGGCACGTACTGCGCATGGGGCCGGGGCAGGCGTTGCAATTGTTTGACGGTAGCAACCAGGTCTTT                                  <  SRR4302543.6730723/94‑1 (MQ=60)
                                                             GCTTTGCGAAGATGCCGCCAACCATATCGGGCACGTACTGCGCATGGGGCCGGGGCAGGCGTTGCAATTGTTTGACGGTAGCAACCAGGTCTTT                                  <  SRR4302543.5518554/94‑1 (MQ=60)
                                                             GCTTTGCGAAGATGCCGCCAACCATATCGGGCACGTACTGCGCATGGGGCCGGGGCAGGCGTTGCAATTGTTTGACGGTAGCAACCAGGTCTTT                                  <  SRR4302543.4607476/94‑1 (MQ=60)
                                                              CTTTGCGAAGATGCCGCCAACCATATCGGGCACGTACTGCGCATGGGGCCGGGGCAGGCGTTGCAATTGTTTGACGGTAGCAACCAGGTCTTTG                                 >  SRR4302543.6854495/1‑94 (MQ=60)
                                                              CTTTGCGAAGATGCCGCCAACCATATCGGGCACGTACTGCGCATGGGGCCGGGGCAGGCGTTGCAATTGTTTGACGGTAGCAACCAGGTCTTTG                                 >  SRR4302543.4938014/1‑94 (MQ=60)
                                                              CTTTGCGAAGATGCCGCCAACCATATCGGGCACGTACTGCGCATGGGGCCGGGGCAGGCGTTGCAATTGTTTGACGGTAGCAACCAGGTCTTTG                                 >  SRR4302543.845337/1‑94 (MQ=60)
                                                              CTTTGCGAAGATGCCGCCAACCATATCGGGCACGTACTGCGCATGGGGCCGGGGCAGGCGTTGCAATTGTTTGACGGTAGCAACCAGGTCTTTG                                 <  SRR4302543.3542737/94‑1 (MQ=60)
                                                              CTTTGCGAAGATGCCGCCAACCATATCGGGCACGTACTGCGCATGGGGCCGGGGCAGGCGTTGCAATTGTTTGACGGTAGCAACCAGGTCTTTG                                 <  SRR4302543.4711294/94‑1 (MQ=60)
                                                              CTTTGCGAAGATGCCGCCAACCATATCGGGCACGTACTGCGCATGGGGCCGGGGCAGGCGTTGCAATTGTTTGACGGTAGCAACCAGGTCTTTG                                 >  SRR4302543.3309279/1‑94 (MQ=60)
                                                              CTTTGCGAAGATGCCGCCAACCATATCGGGCACGTACTGCGCATGGGGCCGGGGCAGGCGTTGCAATTGTTTGACGGTAGCAACCAGGTCTTTG                                 <  SRR4302543.5446181/94‑1 (MQ=60)
                                                                TTGCGAAGATGCCGCCAACCATATCGGGCACGTACTGCGCATGGGGCCGGGGCAGGCGTTGCAATTGTTTGACGGTAGCAACCAGGTCTTTGAC                               <  SRR4302543.2040620/94‑1 (MQ=60)
                                                                TTGCGAAGATGCCGCCAACCATATCGGGCACGTACTGCGCATGGGGCCGGGGCAGGAGTTGCAATTGTTTGACGGTAGCAACCAGGTCTTTGAC                               <  SRR4302543.2450077/94‑1 (MQ=60)
                                                                TGGCGAAGATACCGCCAACCATATCGGGCGCGTCCTGAGCAGGGGGCCGGGGCAGGCATTGCTAATGTTGGACGGTAGCAACCAGGTCTTTGAC                               <  SRR4302543.5564322/94‑1 (MQ=60)
                                                                 TGCGAATATCCCGCCAACCATATCGGGCACGTACTGCGCATGGGGCCGGGGCAGGCGTTGCAATTGTTTGACGGTAGCAACCAGGTCTTTGACG                              <  SRR4302543.4974285/94‑1 (MQ=60)
                                                                 TGCGAAGATGCCGCCAACCATATCGGGCACGTACTGCGCATGGGGCCGGGGCAGGCGTTGCAATTGTTTGACGGTAGCAACCAGGTCTTTGACG                              <  SRR4302543.247444/94‑1 (MQ=60)
                                                                 TGCGAAGATGCCGCCAACCATATCGGGCACGTACTGCGCATGGGGCCGGGGCAGGCGTTGCAATTGTTTGACGGTAGCAACCAGGTCTTTGACG                              <  SRR4302543.422197/94‑1 (MQ=60)
                                                                 TGCGAAGATGCCGCCAACCATATCGGGCACGTACTGCGCATGGGGCCGGGGCAGGCGTTGCAATTGTTTGACGGTAGCAACCAGGTCTTTGACG                              >  SRR4302543.1159010/1‑94 (MQ=60)
                                                                   CGAAGATGCCGCCAACCATATCGGGCACGTACTGCGCATGGGGCCGGGGCAGGCGTTGCAATTGTTTGACGGTAGCAACCAGGTCTTTGACGCC                            >  SRR4302543.7177739/1‑94 (MQ=60)
                                                                   CGAAGATGCCGCCAACCATATCGGGCACGTACTGCGCATGGGGCCGGGGCAGGCGTTGCAATTGTTTGACGGTAGCAACCAGGTCTTTGACGCC                            <  SRR4302543.3284930/94‑1 (MQ=60)
                                                                   CGAAGATGCCGCCAACCATATCGGGCACGTACTGCGCATGGGGCCGGGGCAGGCGTTGCAATTGTTTGACGGTAGCAACCAGGTCTTTGACGCC                            >  SRR4302543.3721805/1‑94 (MQ=60)
                                                                   CGAAGAGGCCGCCAACCATATCGGGCACGTACTGCGCATGGGGCCGGGGCAGGCGTTGCAATTGTTTGACGGTAGCAACCAGGTCTTTGACGCC                            <  SRR4302543.601662/94‑1 (MQ=60)
                                                                    GAAGATGCCGCCAACCATATCGGGCACGTACTGCGCATGGGGCCGGGGCAGGCGTTGCAATTGTTTGACGGTAGCAACCAGGTCTTTGACGCCG                           >  SRR4302543.7323624/1‑94 (MQ=60)
                                                                      AGATGCCGCCAACCATATCGGGCACGTACTGCGCATGGGGCCGGGGCAGGCGTTGCAATTGTTTGACGGTAGCAACCAGGTCTTTGACGCCGAA                         >  SRR4302543.2240354/1‑94 (MQ=60)
                                                                        ATGCCGCCAACCATATCGGGCACGTACTGCGCATGGGGCCGGGGCAGGCGTTGCAATTGTTTGACGGTAGCAACCAGGTCTTTGACGCCGAAAT                       >  SRR4302543.4799481/1‑94 (MQ=60)
                                                                         TGCCGCCAACCATATCGGGCACGTACTGCGCATGGGGCCGGGGCAGGCGTTGCAATTGTTTGACGGTAGCAACCAGGTCTTTGACGCCGAAATT                      <  SRR4302543.1147556/94‑1 (MQ=60)
                                                                         TGCCGCCAACCATATCGGGCACGTACTGCGCATGGGGCCGGGGCAGGCGTTGCAATTGTTTGACGGTAGCAACCAGGTCTTTGACGCCGAAATT                      <  SRR4302543.5847247/94‑1 (MQ=60)
                                                                         TGCCGCCAACCATATCGGGCACGTACTGCGCATGGGGCCGGGGCAGGCGTTGCAATTGTTTGACGGTAGCAACCAGGTCTTTGACGCCGAAATT                      <  SRR4302543.3191567/94‑1 (MQ=60)
                                                                          GCCGCCAACCATATCGGGCACGTACTGCGCATGGGGCCGGGGCAGGCGTTGCAATTGTTTGACGGTAGCAACCAGGTCTTTGACGCCGAAATTA                     >  SRR4302543.2823696/1‑94 (MQ=60)
                                                                            CGCCAACCATATCGGGCGCGTACTGCGCATGGGGCCGGGGCAGGCGTTGCAATTGTTTGACGGTAGCAACCAGGTCTTTGACGCCGAAATTACC                   <  SRR4302543.1179651/94‑1 (MQ=60)
                                                                            CGCCAACCATATCGGGCACGTACTGCGCATGGGGCCGGGGCAGGCGTTGCAATTGTTTGACGGTAGCAACCAGGTCTTTGACGCCGAAATTACC                   <  SRR4302543.2519712/94‑1 (MQ=60)
                                                                            CGCCAACCATATCGGGCACGTACTGCGCATGGGGCCGGGGCAGGCGTTGCAATTGTTTGACGGTAGCAACCAGGTCTTTGACGCCGAAATTACC                   <  SRR4302543.6876228/94‑1 (MQ=60)
                                                                            CGCCAACCATATCGGGCACGTACTGCGCATGGGGCCGGGGCAGGCGTTGCAATTGTTTGACGGTAGCAACCAGGTCTTTGACGCCGAAATTACC                   <  SRR4302543.2246868/94‑1 (MQ=60)
                                                                             GCCAACCATATCGGGCACGTACTGCGCATGGGGCCGGGGCAGGCGTTGCAATTGTTTGACGGTAGCAACCAGGTCTTTGACGCCGAAATTACCA                  <  SRR4302543.6704944/94‑1 (MQ=60)
                                                                             GCCAACCATATCGGGCACGTACTGCGCATGGGGCCGGGGCAGGCGTTGCAATTGTTTGACGGTAGCAACCAGGTCTTTGACGCCGAAATTACCA                  <  SRR4302543.5883410/94‑1 (MQ=60)
                                                                             CCCAACCATATCGGGCACGTACTGCGCATGGGGCCGGGGCAGGCGTTGCAATTGTTTGACGGTAGCAACCAGGTCTTTGACGCCGAAATTACCA                  <  SRR4302543.4314673/94‑1 (MQ=60)
                                                                               CAACCATATCGGGCACGTACTGCGCATGGGGCCGGGGCAGGCGTTGCAATTGTTTGACGGTAGCAACCAGGTCTTTGACGCCGAAATTACCAGC                >  SRR4302543.6359225/1‑94 (MQ=60)
                                                                                AACCATATCGGGCACGTACTGCGCATGGGGCCGGGGCAGGCGTTGCAATTGTTTGACGGTAGCAACCAGGTCTTTGACGCCGAAATTACCAGCG               >  SRR4302543.3236466/1‑94 (MQ=60)
                                                                                AACCATATCGGGCACGTACTGCGCATGGGGCCGGGGCAGGCGTTGCAATTGTTTGACGGTAGCAACCAGGTCTTTGACGCCGAAATTACCAGCG               >  SRR4302543.3727864/1‑94 (MQ=60)
                                                                                  CCATATCGGGCACGTACTGCGCATGGGGCCGGGGCAGGCGTTGCAATTGTTTGACGGTAGCAACCAGGTCTTTGACGCCGAAATTACCAGCGCC             >  SRR4302543.4437009/1‑94 (MQ=60)
                                                                                  CCATATCGGGCACGTACTGCGCATGGGGCCGGGGCAGGCGTTGCAATTGTTTGACGGTAGCAACCAGGTCTTTGACGCCGAAATTACCAGCGCC             >  SRR4302543.660623/1‑94 (MQ=60)
                                                                                    ATATCGGGCACGTACTGCGCATGGGGCCGGGGCAGGCGTTGCAATTGTTTGACGGTAGCAACCAGGTCTTTGACGCCGAAATTACCAGCGCCAG           >  SRR4302543.4452526/1‑94 (MQ=60)
                                                                                    ATATCGGGCACGTACTGCGCATGGGGCCGGGGCAGGCGTTGCAATTGTTTGACGGTAGCAACCAGGTCTTTGACGCCGAAATTACCAGCGCCAG           >  SRR4302543.5577101/1‑94 (MQ=60)
                                                                                     TATCGGGCACGTACTGCGCATGGGGCCGGGGCAGGCGTTGCAATTGTTTGACGGTAGCAACCAGGTCTTTGACGCCGAAATTACCAGCGCCAGC          >  SRR4302543.6617043/1‑94 (MQ=60)
                                                                                      ATCGGGCACGTACTGCGCATGGGGCCGGGGCAGGCGTTGCAATTGTTTGACGGTAGCAATCAGGTCTTTGACGCCGAAATTACCAGCGCCAGCA         >  SRR4302543.4877001/1‑94 (MQ=60)
                                                                                      ATCGGGCACGTACTGCGCATGGGGCCGGGGCAGGCGTTGCAATTGTTTGACGGTAGCAACCAGGTCTTTGACGCCGAAATTACCAGCGCCAGCA         <  SRR4302543.3156797/94‑1 (MQ=60)
                                                                                      ATCGGGCACGTACTGCGCATGGGGCCGGGGCAGGCGTTGCAATTGTTTGACGGTAGCAACCAGGTCTTTGACGCCGAAATTACCAGCGCCAGCA         <  SRR4302543.7361939/94‑1 (MQ=60)
                                                                                       TCGGGCACGTACTGCGCATGGGGCCGGGGCAGGCGTTGCAATTGTTTGACGGTAGCAACCAGGTCTTTGACGCCTAAATTACCAGCGCCAGCAA        >  SRR4302543.1169014/1‑94 (MQ=60)
                                                                                       TCGGGCACGTACTGCGCATGGGGCCGGGGCAGGCGTTGCAATTGTTTGACGGTAGCAACCAGGTCTTTGACGCCGAAATTACCAGCGCCAGCAA        <  SRR4302543.3684906/94‑1 (MQ=60)
                                                                                        CGGGCACGTACTGCGCATGGGGCCGGGGCAGGCGTTGCAATTGTTTGACGGTAGCAACCAGGTCTTTGACGCCGAAATTACCAGCGCCAGCAAA       >  SRR4302543.3066959/1‑94 (MQ=60)
                                                                                        CGGGCACGTACTGCGCATGGGGCCGGGGCAGGCGTTGCAATTGTTTGACGGTAGCAACCAGGTCTTTGACGCCGAAATTACCAGCGCCAGCAAA       <  SRR4302543.2739858/94‑1 (MQ=60)
                                                                                         GGGCACGTACTGCGCATGGGGCCGGGGCAGGCGTTGCAATTGTTTGACGGTAGCAACCAGGTCTTTGACGCCGAAATTACCAGCGCCAGCAAAA      >  SRR4302543.7489188/1‑94 (MQ=60)
                                                                                         GGGCACGTACTGCGCATGGGGCCGGGGCAGGCGTTGCAATTGTTTGACGGTAGCAACCAGGTCTTTGACGCCGAAATTACCAGCGCCAGCAAAA      >  SRR4302543.2216139/1‑94 (MQ=60)
                                                                                           GCACGTACTGCGCATGGGGCCGGGGCAGGCGTTGCAATTGTTTGACGGTAGCAACCAGGTCTTTGACGCCGAAATTACCAGCGCCAGCAAAAAA    <  SRR4302543.1631597/94‑1 (MQ=60)
                                                                                            CACGTACTGCGCATGGGGCCGGGGCAGGCGTTGCAATTGTTTGACGGTAGCAACCAGGTCTTTGACGCCGAAATTACCAGCGCCAGCAAAAAAA   <  SRR4302543.565274/94‑1 (MQ=60)
                                                                                            CACGTACTGCGCATGGGGCCGGGGCAGGCGTTGCAATTGTTTGACGGTAGCAACCAGGTCTTTGACGCCGAAATTACCAGCGCCAGCAAAAAAA   <  SRR4302543.6700165/94‑1 (MQ=60)
                                                                                            CACGTACTGCGCATGGGGCCGGGGCAGGCGTTGCAATTGTTTGACGGTAGCAACCAGGTCTTTGACGCCGAAATTACCAGCGCCAGCAAAAAAA   >  SRR4302543.1748618/1‑94 (MQ=60)
                                                                                             ACGTACTGCGCATGGGGCCGGGGCAGGCGTTGCAATTGTTTGACGGTAGCAACCAGGTCTTTGACGCCGAAATTACCAGCGCCAGCAAAAAAAG  <  SRR4302543.7450688/94‑1 (MQ=60)
                                                                                             ACGTACTGCGCATGGGGCCGGGGCAGGCGTTGCAATTGTTTGACGGTAGCAACCAGGTCTTTGACGCCGAAATTACCAGCGCCAGCAAAAAAAG  <  SRR4302543.3115751/94‑1 (MQ=60)
                                                                                             |                                                                                             
TAACTATGCGTATCCCCCGCATTTATCATCCTGAACCACTGACCAGCCATTCTCACATCGCGCTTTGCGAAGATGCCGCCAACCATATCGGGCGCGTACTGCGCATGGGGCCGGGGCAGGCGTTGCAATTGTTTGACGGTAGCAACCAGGTCTTTGACGCCGAAATTACCAGCGCCAGCAAAAAAAG  >  NC_000913/3089148‑3089334

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑