Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I1 R1	1696	279.3	15874187	97.8%	15524954	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,159,481	A→G	M402T (ATG→ACG)	sufI ←	suppressor of ftsI

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,159,481	0	A	G	99.4%	1007.8 / ‑3.6	328	M402T (ATG→ACG)	sufI	suppressor of ftsI
Reads supporting (aligned to +/- strand): ref base A (1/1); new base G (143/183); total (144/184)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.69e-01

Only 100 of 342 total aligned reads displayed.

AGCTCCACTTGTCCGTCAACCCAAACGGTATCTTTCCAGCCTCTGTCTTCCGGGAACGGCATCGCGCCATTCACGTTACGGATCTGGAACATTACGCCTTCAATATGGAACGCTTGCGGCTCGTCCGCGCGTACCGTCCAGCG‑TTCCCACGTTCCTTGCTGCGCGGTGACATCAATACGG  >  NC_000913/3159391‑3159570

agcTCCACTTGTCCGTCACCCCAAACGGTAGCTTTCCAGCCTCTGTCTTCCGGGAACGGCATCGCGCCATTCACGTTACGGATCTGGAACGtt                                                                                          <  1:7904914/93‑1 (MQ=255)

agcTCCACTTGTCCGTCAACCCAAACGGTATCTTTCCAGCCTCTGTCTTCCGGGAACGGCATCGCGCCATTCACGTTACGGATCTGGAACGtt                                                                                          >  1:130181/1‑93 (MQ=255)

 gcTCCACTTGTCCGTCAACCCAAACGGTATCTTTCCAGCCTCTGTCTTCCGGGAACGGCATCGCGCCATTCACGTTACGGATCTGGAACGTTa                                                                                         >  2:4892863/1‑93 (MQ=255)

 gcTCCACTTGTCCGTCAACCCAAACGGTATCTTTCCAGCCTCTGTCTTCCGGGAACGGCATCGCGCCATTCACGTTACGGATCTGGAACGTTa                                                                                         <  1:7755072/93‑1 (MQ=255)

 gcTCCACTTGTCCGTCAACCCAAACGGTATCTTCCCAGCCTCTGTCTTCCGGGAACGGCATCGCGCCATTCACGTTACGGATCTGGAACGTTa                                                                                         <  1:2915144/93‑1 (MQ=255)

  cTCCACTTGTCCGTCAACCCAAACGGTATCTTTCCAGCCTCTGTCTTCCGGGAACGGCATCGCGCCATTCACGTTACGGATCTGGAACGTTAc                                                                                        <  1:4492814/93‑1 (MQ=255)

  cTCCACTTGTCCGTCAACCCAAACGGTATCTTTCCAGCCTCTGTCTTCCGGGAACGGCATCGCGCCATTCACGTTACGGATCTGGAACGTTAc                                                                                        >  1:2995231/1‑93 (MQ=255)

   tCCACTTGTCCGTCAACCCAAACGGTATCTTTCCAGCCTCTGTCTTCCGGGAACGGCATCGCGCCATTCACGTTACGGATCTGGAACGTTACg                                                                                       >  1:4000149/1‑93 (MQ=255)

   tCCACTTGTCCGTCAACCCAAACGGTATCTTTCCAGCCTCTGTCTTCCGGGAACGGCATCGCGCCATTCACGTTACGGATCTGGAACGTTACg                                                                                       >  2:6307436/1‑93 (MQ=255)

   tCCACTTGTCCGTCAACCCAAACGGTATCTTTCCAGCCTCTGTCTTCCGGGAACGGCATCGCGCCATTCACGTTACGGATCTGGAACGTTACg                                                                                       >  2:2458241/1‑93 (MQ=255)

    ccACTTGTCCGTCAACCCAAACGGTATCTTTCCAGCCTCTGTCTTCCGGGAACGGCATCGCGCCATTCACGTTACGGATCTGGAACGTTACGc                                                                                      >  1:6760017/1‑93 (MQ=255)

     cACTTGTCCGTCAACCCAAACGGTATCTTTCCAGCCTCTGTCTTCCGGGAACGGCATCGCGCCATTCACGTTACGGATCTGGAACGTTACGcc                                                                                     >  1:6538023/1‑93 (MQ=255)

      aCTTGTCCGTCAACCCAAACGGTATCTTTCCAGCCTCTGTCTTCCGGGAACGGCATCGCGCCATTCACGTTACGGATCTGGAACGTTACGCCt                                                                                    >  2:7623451/1‑93 (MQ=255)

      aCTTGTCCGTCAACCCAAACGGTATCTTTCCAGCCTCTGTCTTCCGGGAACGGCATCGCGCCATTCACGTTACGGATCTGGAACGTTACGCCt                                                                                    >  2:1242196/1‑93 (MQ=255)

      aCTTGTCCGTCAACCCAAACGGTATCTTTCCAGCCTCTGTCTTCCGGGAACGGCATCGCGCCATTCACGTTACGGATCTGGAACGTTACGCCt                                                                                    >  2:4584087/1‑93 (MQ=255)

       cTTGTCCGTCAACCCAAACGGTATCTTTCCAGCCTCTGTCTTCCGGGAACGGCATCGCGCCATTCACGTTACGGATCTGGAACGTTACGCCtt                                                                                   >  1:885847/1‑93 (MQ=255)

       cTTGTCCGTCAACCCAAACGGTATCTTTCCAGCCTCTGTCTTCCGGGAACGGCATCGCGCCATTCACGTTACGGATCTGGAACGTTACGCCtt                                                                                   >  2:5671983/1‑93 (MQ=255)

        ttGTCCGTCAACCCAAACGGTATCTTTCCAGCCTCTGTCTTCCGGGAACGGCATCGCGCCATTCACGTTACGGATCTGGAACGTTACGCCTTc                                                                                  >  1:7360469/1‑93 (MQ=255)

        ttGTCCGTCAACCCAAACGGTATCTTTCCAGCCTCTGTCTTCCGGGAACGGCATCGCGCCATTCACGTTACGGATCTGGAACGTTACGCCTTc                                                                                  >  1:6924192/1‑93 (MQ=255)

        ttGTCCGTCAACCCAAACGGTATCTTTCCAGCCTCTGTCTTCCGGGAACGGCATCGCGCCATTCACGTTACGGATCTGGAACGTTACGCCTTc                                                                                  >  2:3760005/1‑93 (MQ=255)

        ttGTCCGTCAACCCAAACGGTATCTTTCCAGCCTCTGTCTTCCGGGAACGGCATCGCGCCATTCACGTTACGGATCTGGAACGTTACGCCTTc                                                                                  >  1:1097365/1‑93 (MQ=255)

         tGTCCGTCAACCCAAACGGTATCTTTCCAGCCTCTGTCTTCCGGGAACGGCATCGCGCCATTCACGTTACAGATCTGGAACGTTACGCCTTCa                                                                                 >  2:6451145/1‑93 (MQ=255)

           tCCGTCAACCCAAACGGTATCTTTCCAGCCTCTGTCTTCCGGGAACGGCATCGCGCCATTCACGTTACGGATCTGGAACGTTACGCCTTCAat                                                                               >  2:1794283/1‑93 (MQ=255)

           tCCGTCAACCCAAACGGTATCTTTCCAGCCTCTGTCTTCCGGGAACGGCATCGCGCCATTCACGTTACGGATCTGGAACGTTACGCCTTCAat                                                                               >  2:735943/1‑93 (MQ=255)

            ccGTCACCCCAAACGGTATCTTTCCAGCCTCTGTCTTCCGGGAACGGCATCGCGCCATTCACGTTACGGATCTGGAACGTTACGCCTTCAata                                                                              <  1:4226861/93‑1 (MQ=255)

               tCAACCCAAACGGTATCTTTCCAGCCTCTGTCTTCCGGGAACGGCATCGCGCCATTCACGTTACGGATCTGGAACGTTACGCCTTCAATATgg                                                                           >  2:5558998/1‑93 (MQ=255)

                cAACCCAAACGGTATCTTTCCAGCCTCTGTCTTCCGGGAACGGCATCGCGCCATTCACGTTACGGATCTGGAACGTTACGCCTTCAATATGGa                                                                          <  2:7500065/93‑1 (MQ=255)

                cAACCCAAACGGTATCTTTCCAGCCTCTGTCTTCCGGGAACGGCATCGCGCCATTCACGTTACGGATCTGGAACGTTACGCCTTCAATATGGa                                                                          <  1:1862232/93‑1 (MQ=255)

                 accccAAACGGTATCTTTCCAGCCTCTGTCTTCCGGGAACGGCATCGCGCCATTCACGTTACGGATCTGGAACGTTACGCCTTCAATATGGaa                                                                         <  2:1339484/91‑1 (MQ=255)

                 aaCCCAAACGGTATTTTTCCAGCCTCTGTCTTCCGGGAACGGCATCGCGCCATTCACGTTACGGATCTGGAACGTTACGCCTTCAATATGGaa                                                                         <  1:2611696/93‑1 (MQ=255)

                 aaCCCAAACGGTATCTTTCCAGCCTCTGTCTTCCGGGAACGGCATCGCGCCATTCACGTTACGGATCTGGAACGTTACGCCTTCAATATGGaa                                                                         <  2:4343077/93‑1 (MQ=255)

                  aCCCAAACGGTATCTTTCCAGCCTCTGTCTTCCGGGAACGGCATCGCGCCATTCACGTTACGGATCTGGAACGTTACGCCTTCAATATGGAAc                                                                        <  1:5162066/93‑1 (MQ=255)

                    ccAAACGGTATCTTTCCAGCCTCTGTCTTCCGGGAACGGCATCGCGCCATTCACGTTACGGATCTGGAACGTTACGCCTTCAATATGGAACGc                                                                      <  1:534706/93‑1 (MQ=255)

                    ccAAACGGTATCTTTCCAGCCTCTGTCTTCCGGGAACGGCATCGCGCCATTCACGTTACGGATCTGGAACGTTACGCCTTCAATATGGAACGc                                                                      <  1:1538686/93‑1 (MQ=255)

                     cAAACGGTATCTTTCCAGCCTCTGTCTTCCGGGAACGGCATCGCGCCATTCACGTTACGGATCTGGAACGTTACGCCTTCAATATGGAACGCt                                                                     >  1:6002703/1‑93 (MQ=255)

                     cAAACGGTATCTTTCCAGCCTCTGTCTTCCGGGAACGGCATCGCGCCATTCACGTTACGGATCTGGAACGTTACGCCTTCAATATGGAACGCt                                                                     >  1:4982904/1‑93 (MQ=255)

                     cAAACGGTATCTTTCCAGCCTCTGTCTTCCGGGAACGGCATCGCGCCATTCACGTTACGGATCTGGAACGTTACGCCTTCAATATGGAACGCt                                                                     <  2:7459701/93‑1 (MQ=255)

                     cAAACGGTATCTTTCCAGCCTCTGTCTTCCGGGAACGGCATCGCGCCATTCACGTTACGGATCTGGAACGTTACGCCTTCAATATGGAACGCt                                                                     >  2:6573975/1‑93 (MQ=255)

                      aaaCGGTATCTTTCCAGCCTCTGTCTTCCGGGAACGGCATCGCGCCATTCACGTTACGGATCTGGAACGTTACGCCTTCAATATGGAACGCtt                                                                    >  1:1815416/1‑93 (MQ=255)

                       agcGGTATCTTTCCAGCCTCTGTCTTCCGGGAACGGCATCGCGCCATTCACGTTACGGATCTGGAACGTTACGCCTTCAATATGGAACGCTTg                                                                   <  1:7662209/91‑1 (MQ=255)

                       aaCGGTATCTTTCCAGCCTCTGTCTTCCGGGAACGGCATCGCGCCATTCACGTTACGGATCTGGAACGTTACGCCTTCAATATGGAACGCTTg                                                                   <  2:7174660/93‑1 (MQ=255)

                        aCGGTATCTTCCCAGCCTCTGTCTTCCGGGAACGGCATCGCGCCATTCACGTTACGGATCTGGAACGTTACGCCTTCAATATGGAACGCTTGc                                                                  <  1:484374/93‑1 (MQ=255)

                         cGGTATCTTTCCAGCCTCTGTCTTCCGGGAACGGCATCGCGCCATTCACGTTACGGATCTGGAACGTTACGCCTTCAATATGGAACGCTTGCg                                                                 <  2:41420/93‑1 (MQ=255)

                         cGGTATCTTTCCAGCCTCTGTCTTCCGGGAACGGCATCGCGCCATTCACGTTACGGATCTGGAACGTTACGCCTTCAATATGGAACGCTTGCg                                                                 >  1:5734616/1‑93 (MQ=255)

                          ggTATCTTTCCAGCCTCTGTCTTCCGGGAACGGCATCGCGCCATTCACGTTACGGATCTGGAACGTTACGCCTTCAATATGGAACGCTTGCgg                                                                <  2:10160/93‑1 (MQ=255)

                          ggTATCTTTCCAGCCTCTGTCTTCCGGAAACGGCATCGCGCCATTCACGTTACGGATCTGGAACGTTACGCCTTCAATATGGAACGCTTGCgg                                                                <  2:3415268/93‑1 (MQ=255)

                           gTATCTTTCCAGCCTCTGTCTTCCGGGAACGGCATCGCGCCATTCACGTTACGGATCTGGAACGTTACGCCTTCAATATGGAACGCTTGCGGc                                                               >  2:7114325/1‑93 (MQ=255)

                            tATCTTTCCAGCCTCTGTCTTCCGGGAACGGCATCGCGCCATTCACGTTACGGATCTGGAACGTTACGCCTTCAATATGGAACGCTTGCGGCt                                                              >  1:5314810/1‑93 (MQ=255)

                              tCTTTCCAGCCTCTGTCTTCCGGGAACGGCATCGCGCCATTCACGTTACGGATCTGGAACGTTACGCCTTCAATATGGAACGCTTGCGGCTcc                                                            >  2:6432586/1‑92 (MQ=255)

                               ttttCCAGCCTCTGTCTTCCGGGAACGGCATCGCGCCATTCACGTTACGGATCTGGAACGTTACGCCTTCAATATGGAACGCTTGCGGCTCGt                                                           <  1:1132969/92‑1 (MQ=255)

                               cTTTCCAGCCTCTGTCTTCCGGGAACGGCATCGCGCCATTCACGTTACGGATCTGGAACGTTACGCCTTCAATATGGAACGCTTGCGGCTccc                                                           >  2:4005706/1‑91 (MQ=255)

                                tttCCAGCCTCTGTCTTCCGGGAACGGCATCGCGCCATTCACGTTACGGATCTGGAACGTTACGCCTTCAATATGGAACGCTTGCGGctcctc                                                          >  2:7715984/1‑93 (MQ=255)

                                tttCCAGCCTCTGTCTTCCGGGAACGGCATCGCGCCATTCACGTTACGGATCTGGAACGTTACGCCTTCAATATGGAACGCTTGCGGCTCGTc                                                          <  1:5639020/93‑1 (MQ=255)

                                 ttCCAGCCTCTGTCTTCCGGGAACGGCATCGCGCCATTCACGTTACGGATCTGGAACGTTACGCCTTCAATATGGAACGCTTGCGGCTCGttc                                                         >  1:5863552/1‑91 (MQ=255)

                                 ttCCAGCCTCTGTCTTCCGGGAACGGCATCGCGCCATTCACGTTACGGATCTGGAACGTTACGCCTTCAATATGGAACGCTTGCGGCTCGTcc                                                         <  1:7298581/93‑1 (MQ=255)

                                     aGCCTCTGTCTTCCGGGAACGGCATCGCGCCATTCACGTTACGGATCTGGAACGTTACGCCTTCAATATGGAACGCTTGCGGCTCGTCcgcgc                                                     <  2:6091175/93‑1 (MQ=255)

                                     aGCCTCTGTCTTCCGGGAACGGCATCGCGCCATTCACGTTACGGATCTGGAACGTTACGCCTTCAATATGGAACGCTTGCGGCTCGTCcgcgc                                                     <  2:2811117/93‑1 (MQ=255)

                                      gCCTCTGTCTTCCGGGAACGGCATCGCGCCATTCACGTTACGGATCTGGAACGTTACGCCTTCAATATGGAACGCTTGCGGCTCCTCcccgcg                                                    >  1:3017369/1‑93 (MQ=255)

                                         tctGTCTTCCGGGAACGGCATCGCGCCATTCACGTTACGGATCTGGAACGTTACGCCTTCAATATGGAACGCTTGCGGCTCCTCcgcccgcgc                                                 >  2:2606050/1‑90 (MQ=255)

                                           tGTCTTCCGGGAACGGCATCGCGCCATTCACGTTACGGATCTGGAACGTTACGCCTTCAATATGGAACGCTTGCGGCTCCCCCCCGCGGACCg                                               >  1:4306794/1‑93 (MQ=255)

                                               ttCCGGGAACGGCATCGCGCCATTCACGTTACGGATCTGGAACGTTACGCCTTCAATATGGAACGCTTGCGGCTCGTCCGCGCGTACCGTCCa                                           <  2:237924/93‑1 (MQ=255)

                                                tCCGGGAACGGCATCGCGCCATTCACGTTACGGATCTGGAACGTTACGCCTTCAATATGGAACGCTTGCGGCTCGTCCGCGCGTACCGTCCAg                                          <  2:1937652/93‑1 (MQ=255)

                                                 ccGGGAACGGCATCGCGCCATTCACGTTACGGATCTGGAACGTTACGCCTTCAATATGGAACGCTTGCGGCTCGCCCGCCCGTACCGTcccac                                         >  2:3040444/1‑90 (MQ=255)

                                                  cGGGAACGGCATCGCGCCATTCACGTTACGGATCTGGAACGTTACGCCTTCAATATGGAACGCTTGCGGCTCGTCCGCGCGTACCGTCCAGCg                                        <  1:2200756/93‑1 (MQ=255)

                                                      aaCGGCATCGCGCCATTCACGTTACGGATCTGGAACATTACGCCTTCAATATGGAACGCTTGCGGCTCCTCCCCCCCGACCCTCCAGCggttc                                    >  1:5473770/1‑89 (MQ=255)

                                                       aCGGCATCGCGCCATTCACGTTACGGATCTGGAACGTTACGCCTTCAATATGGAACGCTTGCGGCTCGTCCGCGCGTACCGTCCAGCG‑TTccc                                  <  2:7254616/93‑1 (MQ=255)

                                                        cGGCATCGCTCCATTCACGTTAGGGAACTGGAACGTTACGCCTTCAATATGGAACGCTTGCGGCTCGTCCGCGCGTACCGTCCAGCG‑TTCCCa                                 <  2:2349734/93‑1 (MQ=255)

                                                         ggCATCGCGCCATTCACGTTACGGATCTGGAACGTTACGCCTTCAATATGGAACGCTTGCGGCTCGTCCGCGCGTACCGTCCAGCG‑TTCCCAc                                <  1:214582/93‑1 (MQ=255)

                                                          gCATCGCGCCATTCACGTTATGGATCTGGAACGTTACGCCTTCAATATGGAACGCTTGCGGCTCCGCCCCGCCGACCGTCCACCGCTTccccc                                >  2:1885255/1‑91 (MQ=255)

                                                           cATCGCGCCATTCACGTTACGGATCTGGAACGTTACGCCTTCAATATGGAACGCTTGCGGCTCGTCCGCGCGTACCGTCCAGCG‑TTCCCACGt                              <  2:362480/93‑1 (MQ=255)

                                                               gcgcCATTCACGTTACGGATCTGGAACGTTACGCCTTCAATATGGAACGCTTGCGGCTCGTCCGCGCGTACCGTCCAGCG‑TTCCCACGTTCCt                          <  1:362965/93‑1 (MQ=255)

                                                               gcgcCATTCACGTTACGGATCTGGAACGTTACGCCTTCAATATGGAACGCTTGCGGCTCGTCCGCGCGTACCGTCCAGCG‑TTCCCACGTTCCt                          <  1:3279949/93‑1 (MQ=255)

                                                               gcgcCATTCACGTTACGGATCTGGAACGTTACGCCTTCAATATGGAACGCTTGCGGCTCGTCCGCGCGTACCGTCCAGCG‑TTCCCACGTTCCt                          <  1:7512761/93‑1 (MQ=255)

                                                               ccgcCATTCACGTTACGGATCTGGAACGTTACGCCTTCAATATGGAACGCTTGCGGCTCGTCCGCGCGTACCGTCCAGCG‑TTCCCACGTTCCt                          <  2:6758782/92‑1 (MQ=255)

                                                                cgcCATTCACGTTACGGATCTGGAACGTTACGCCTTCAATATGGAACGCTTGCGGCTCGTCCGCGCGTACCGTCCAGCG‑TTCCCACGTTCCtt                         <  2:783057/93‑1 (MQ=255)

                                                                  ccATTCACGTTACGGATCTGGAACGTTACGCCTTCAATATGGAACGCTTGCGGCTCCTCCGCGCGCAGCGTCCCGCG‑GTTCCACCTTcccttc                       >  2:5293896/1‑89 (MQ=255)

                                                                     ttCACGTTACGGATCTGGAACGTTACGCCTTCAATATGGAACGCTTGCGGCTCGTCCGCGCGTACCGTCCAGCG‑TTCCCACGTTCCTtgctgc                    <  2:4996972/93‑1 (MQ=255)

                                                                     ttCACGTTACGGATCTGGAACGTTACGCCTTCAATATGGAACGCTTGCGGCTCGTCCCCGCGTACCGTCCAGCG‑GTTCCCCCCTCCTttctgc                    >  1:2815441/1‑93 (MQ=255)

                                                                     ttCACGTTACGGATCTGGAACGTTACGCCTTCAATATGGAACGCTTGCGGCTCCTCCGCGCGGACCGTCCACCG‑GTCCCCCGTTCCTtgctgc                    >  1:1722248/1‑93 (MQ=255)

                                                                     ttCACGTTACGGAGCTGGAACGTTACGCCTTCAATATGGAACGCTTGCGGCTCGGCCGCGCGTACCGTCCAGCG‑TTCCCACGTTCCTtgctgc                    <  1:6382462/93‑1 (MQ=255)

                                                                       cACGTTACGGATCTGGACCGTTACGCCTTCAATATGGAACGCTTGCGGCTCGTCCGCGCGTACCGTCCAGCG‑TTCCCACGTTCCTTGCTgcgc                  <  2:4279892/93‑1 (MQ=255)

                                                                        aCGTTACGGATCTGGAACGTTACGCCTTCAATATGGAACGCTTGCGGCTCCTCCGCGCGTACCGTCCAGCG‑TTCCCACCCTCCTTGCTGcgcg                 >  2:4733570/1‑93 (MQ=255)

                                                                           ttACGGATCTGGAACGTTACGCCTTCAATATGGAACGCTTGCGGCTCGTCCGCGCGTACCGTCCAGCG‑TTCCCACGTTCCTTGCTGCGCGGTg              <  2:4815781/93‑1 (MQ=255)

                                                                            tACGGATCTGGAACGTTACGCCTTCAATATGGAACGCTTGCGGCTCGTCCGCGCGTACCGTCCAGCG‑TTCCCACGTTCCTTGCTGCGCGGTGa             <  2:3920334/93‑1 (MQ=255)

                                                                              cGGATCTGGAACGTTACGCCTTCAATATGGAACGCTTGCGGCTCGTCCGCGCGTACCGTCCAGCG‑TTCCCACGTTCCTTGCTGCGCGGTGACa           <  1:6241708/93‑1 (MQ=255)

                                                                               ggATCTGGAACGTTACGCCTTCAATATGGAACGCTTGCGGCTCGTCCGCGCGTACCGTCCAGCG‑TTCCCACGTTCCTTGCTGCGCGGTGACAt          <  1:1028390/93‑1 (MQ=255)

                                                                                gATCTGGAACGTTACGCCTTCAATATGGAACGCTTGCGGCTCGTCCGCGCGTACCGTCCAGCG‑TTCCCACGTTCCTTGCTGCGCGGTGACATc         <  2:2859597/93‑1 (MQ=255)

                                                                                  tCTGGAACGTTACGCCTTCAATATGGAACGCTTGCGGCTCGTCCGCGCGTACCGTCCAGCG‑TTCCCACGTTCCTTGCTGCGCGGTGACATCaa       <  1:3169775/93‑1 (MQ=255)

                                                                                    tGGAACGTTACGCCTTCAATATGGAACGCTTGCGGCTCGTCCGCGCGTACCGTCCAGCG‑TTCCCACGTTCCTTGCTGCGCGGTGACATCAATa     <  2:1568497/93‑1 (MQ=255)

                                                                                    tGGAACGTTACGCCTTCAATATGGAACGCTTGCGGCTCGTCCGCGCGTACCGTCCAGCG‑TTCCCACGTTCCTTGCTGCGCGGTGACATCAATa     <  2:5858347/93‑1 (MQ=255)

                                                                                    tGGAACGTTACGCCTTCAATATGGAACGCTTGCGGCTCGTCCGCGCGTACCGTCCAGCG‑TTCCCACGTTCCTTGCTGCGCGGTGACATCAATa     <  2:3734980/93‑1 (MQ=255)

                                                                                    tGGAACGTTACGCCTTCAATATGGAACGCTTGCGGCTCGTCCGCGCGTACCGTCCAGCG‑TTCCCACGTTCCTTGCTGCGCGGTGACATCAATa     <  1:4878739/93‑1 (MQ=255)

                                                                                    tGGAACGTTACGCCTTCAATATGGAACGCTTGCGGCTCGTCCGCGCGTACCGTCCAGCG‑TTCCCACGTTCCTTGCTGCGCGGTGACATCAATa     <  1:337325/93‑1 (MQ=255)

                                                                                    tGGAACGTTACGCCTTCAATATGGAACGCTTGCGGCTCGTCCGCGCGTACCGTCCAGCG‑TTCCCACGTTCCTTGCTGCGCGGTGACATCAATa     <  2:5391/93‑1 (MQ=255)

                                                                                    tGGAACGTTACGCCTTCAATATGGAACGCTTGCGGCTCGTCCGCGCGTACCGTCCAGCG‑TTCCCACGTTCCTTGCTGCGCGGTGACATCAATa     <  2:1119869/93‑1 (MQ=255)

                                                                                     ggAACGTTACGCCTTCAATATGGAACGCTTGCGGCTCGTCCGCGCGTACCGTCCAGCG‑TTCCCACGTTCCTTGCTGCGCGGTGACATCAATAc    <  2:7056666/93‑1 (MQ=255)

                                                                                     ggAACGTTACGCCTTCAATATGGAACGCTTGCGGCTCGTCCGCGCGTACCGTCCAGCG‑TTCCCACGTTCCTTGCTGCGCGGTGACATCAATAc    <  1:1239790/93‑1 (MQ=255)

                                                                                      gAACGTTACGCCTTCAATATGGAACGCTTGCGGCTCCTCCCCGCGTACCGTCCAGCG‑TTCCCACGTTCCTTGCTGCGCGGGGGCATCAATACg   >  2:2682656/1‑93 (MQ=255)

                                                                                       aaCGTTACGCCTTCAATATGGAACGCTTGCGGCTCGTCCGCGCGTACCGTCCAGCG‑TTCCCACGTTCCTTGCTGCGCGGTGACATCAATACgg  <  1:1604111/93‑1 (MQ=255)

                                                                                       aaCGTTACGCCTTCAATATGGAACGCTTGCGGCTCGCCCGCGCGGACCGTCCCGCG‑GTCCCCCCTTCCTTTCTGCGCGGGGGCATCAATAAgg  >  2:215767/1‑93 (MQ=255)

AGCTCCACTTGTCCGTCAACCCAAACGGTATCTTTCCAGCCTCTGTCTTCCGGGAACGGCATCGCGCCATTCACGTTACGGATCTGGAACATTACGCCTTCAATATGGAACGCTTGCGGCTCGTCCGCGCGTACCGTCCAGCG‑TTCCCACGTTCCTTGCTGCGCGGTGACATCAATACGG  >  NC_000913/3159391‑3159570

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 20 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output Only 200 of 210 total aligned reads displayed.

AGCAGCTCCACTTGTCCGTCAACCCAAACGGTATCTTTCCAGCCTCTGTCTTCCGGGAACGGCATCGCGCCATTCACGTTACGGATCTGGAACATTACGCCTTCAATATGGAACGCTTGCGGCTCGTCCGCGCGTACCGTCCAGCGTTCCCACGTTCCTTGCTGCGCGGTGACATCAATACGGTTGA  >  NC_000913/3159388‑3159574

AGCAGCTCCACTTGTCCGTCAACCCAAACGGTATCTTTCCAGCCTCTGTCTTCCGGGAACGGCATCGCGCCATTCACGTTACGGATCTGGAACG                                                                                               <  SRR4302543.3226298/94‑1 (MQ=60)

AGCAGCTCCACTTGTCCGTCAACCCAAACGGTATCTTTCCAGCCTCTGTCTTCCGGGAACGGCATCGCGCCATTCACGTTACGGATCTGGAACG                                                                                               <  SRR4302543.3226300/94‑1 (MQ=60)

AGCAGCTCCACTTGTCCGTCAACCCAAACGGTATCTTTCCAGCCTCTGTCTTCCGGGAACGGCATCGCGCCATTCACGTTACGGATCTGGAACG                                                                                               <  SRR4302543.4352596/94‑1 (MQ=60)

 CCAGCTCCACTTGTCCGTCACCCCAACCGGTATCTTTCCAGCCTCTGTCTTCCGGGAACGGCATCGCGCCATTCACGTTACGGATCTGGAACGT                                                                                              <  SRR4302543.7023713/94‑1 (MQ=60)

  CAGCTCCACTTGTCCGTCAACCCAAACGGTATCTTTCCAGCCTCTGTCTTCCGGGAACGGCATCGCGCCATTCACGTTACGGATCTGGAACGTT                                                                                             >  SRR4302543.131347/1‑94 (MQ=60)

  CAGCTCCACTTGTCCGTCAACCCAAACGGTATCTTTCCAGCCTCTGTCTTCCGGGAACGGCATCGCGCCATTCACGTTACGGATCTGGAACGTT                                                                                             >  SRR4302543.7758572/1‑94 (MQ=60)

  CAGCTCCACTTGTCCGTCAACCCAAACGGTATCTTTCCAGCCTCTGTCTTCCGGGAACGGCATCGCGCCATTCACGTTACGGATCTGGAACGTT                                                                                             <  SRR4302543.3588825/94‑1 (MQ=60)

  CAGCTCCACTTGTCCGTCAACCCAAACGGTATCTTTCCAGCCTCTGTCTTCCGGGAACGGCATCGCGCCATTCACGTTACGGATCTGGAACGTT                                                                                             <  SRR4302543.1296979/94‑1 (MQ=60)

   AGCTCCACTTGTCCGTCACCCCAAACGGTAGCTTTCCAGCCTCTGTCTTCCGGGAACGGCATCGCGCCATTCACGTTACGGATCTGGAACGTTA                                                                                            <  SRR4302543.7979198/94‑1 (MQ=60)

    GCTCCACTTGTCCGTCAACCCAAACGGTATCTTTCCAGCCTCTGTCTTCCGGGAACGGCATCGCGCCATTCACGTTACGGATCTGGAACGTTAC                                                                                           >  SRR4302543.1130018/1‑94 (MQ=60)

    GCTCCACTTGTCCGTCAACCCAAACGGTATCTTTCCAGCCTCTGTCTTCCGGGAACGGCATCGCGCCATTCACGTTACGGATCTGGAACGTTAC                                                                                           >  SRR4302543.1767195/1‑94 (MQ=60)

    GCTCCACTTGTCCGTCAACCCAAACGGTATCTTTCCAGCCTCTGTCTTCCGGGAACGGCATCGCGCCATTCACGTTACGGATCTGGAACGTTAC                                                                                           <  SRR4302543.429997/94‑1 (MQ=60)

    GCTCCACTTGTCCGTCAACCCAAACGGTATCTTTCCAGCCTCTGTCTTCCGGGAACGGCATCGCGCCATTCACGTTACGGATCTGGAACGTTAC                                                                                           <  SRR4302543.3926759/94‑1 (MQ=60)

    GCTCCACTTGTCCGTCAACCCAAACGGTATCTTTCCAGCCTCTGTCTTCCGGGAACGGCATCGCGCCATTCACGTTACGGATCTGGAACGTTAC                                                                                           <  SRR4302543.7828281/94‑1 (MQ=60)

    GCTCCACTTGTCCGTCAACCCAAACGGTATCTTTCCAGCCTCTGTCTTCCGGGAACGGCATCGCGCCATTCACGTTACGGATCTGGAACGTTAC                                                                                           >  SRR4302543.6271184/1‑94 (MQ=60)

    GCTCCACTTGTCCGTCAACCCAAACGGTATCTTTCCAGCCTCTGTCTTCCGGGAACGGCATCGCGCCATTCACGTTACGGATCTGGAACGTTAC                                                                                           >  SRR4302543.2818375/1‑94 (MQ=60)

    GCTCCACTTGTCCGTCAACCCAAACGGTATCTTTCCAGCCTCTGTCTTCCGGGAACGGCATCGCGCCATTCACGTTACGGATCTGGAACGTTAC                                                                                           >  SRR4302543.3022408/1‑94 (MQ=60)

    GCTCCACTTGTCCGTCAACCCAAACGGTATCTTTCCAGCCTCTGTCTTCCGGGAACGGCATCGCGCCATTCACGTTACGGATCTGGAACGTTAC                                                                                           >  SRR4302543.3296561/1‑94 (MQ=60)

    GCTCCACTTGTCCGTCAACCCAAACGGTATCTTCCCAGCCTCTGTCTTCCGGGAACGGCATCGCGCCATTCACGTTACGGATCTGGAACGTTAC                                                                                           <  SRR4302543.2941634/94‑1 (MQ=60)

     CTCCACTTGTCCGTCAACCCAAACGGTATCTTTCCAGCCTCTGTCTTCCGGGAACGGCATCGCGCCATTCACGTTACGGATCTGGAACGTTACG                                                                                          >  SRR4302543.6868848/1‑94 (MQ=60)

     CTCCACTTGTCCGTCAACCCAAACGGTATCTTTCCAGCCTCTGTCTTCCGGGAACGGCATCGCGCCATTCACGTTACGGATCTGGAACGTTACG                                                                                          >  SRR4302543.4035825/1‑94 (MQ=60)

     CTCCACTTGTCCGTCAACCCAAACGGTATCTTTCCAGCCTCTGTCTTCCGGGAACGGCATCGCGCCATTCACGTTACGGATCTGGAACGTTACG                                                                                          <  SRR4302543.1862652/94‑1 (MQ=60)

     CTCCACTTGTCCGTCAACCCAAACGGTATCTTTCCAGCCTCTGTCTTCCGGGAACGGCATCGCGCCATTCACGTTACGGATCTGGAACGTTACG                                                                                          <  SRR4302543.4533642/94‑1 (MQ=60)

      TCCACTTGTCCGTCAACCCAAACGGTATCTTTCCAGCCTCTGTCTTCCGGGAACGGCATCGCGCCATTCACGTTACGGATCTGGAACGTTACGC                                                                                         >  SRR4302543.6823862/1‑94 (MQ=60)

      TCCACTTGTCCGTCAACCCAAACGGTATCTTTCCAGCCTCTGTCTTCCGGGAACGGCATCGCGCCATTCACGTTACGGATCTGGAACGTTACGC                                                                                         >  SRR4302543.6115276/1‑94 (MQ=60)

       CCACTTGTCCGTCAACCCAAACGGTATCTTTCCAGCCTCTGTCTTCCGGGAACGGCATCGCGCCATTCACGTTACGGATCTGGAACGTTACGCC                                                                                        >  SRR4302543.6599570/1‑94 (MQ=60)

       CCACTTGTCCGTCAACCCAAACGGTATCTTTCCAGCCTCTGTCTTCCGGGAACGGCATCGCGCCATTCACGTTACGGATCTGGAACGTTACGCC                                                                                        >  SRR4302543.4955043/1‑94 (MQ=60)

        CACTTGTCCGTCAACCCAAACGGTATCTTTCCAGCCTCTGTCTTCCGGGAACGGCATCGCGCCATTCACGTTACGGATCTGGAACGTTACGCCT                                                                                       >  SRR4302543.7900761/1‑94 (MQ=60)

        CACTTGTCCGTCAACCCAAACGGTATCTTTCCAGCCTCTGTCTTCCGGGAACGGCATCGCGCCATTCACGTTACGGATCTGGAACGTTACGCCT                                                                                       >  SRR4302543.7764340/1‑94 (MQ=60)

        CACTTGTCCGTCAACCCAAACGGTATCTTTCCAGCCTCTGTCTTCCGGGAACGGCATCGCGCCATTCACGTTACGGATCTGGAACGTTACGCCT                                                                                       >  SRR4302543.2952187/1‑94 (MQ=60)

         cgTTGTCCGTCACCCCAAACGGTATCTTTCCAGCCTCTGTCTTCCGGGAACGGCATCGCGCCATTCACGTTACGGATCTGGAACGTTACGCCTT                                                                                      <  SRR4302543.5959952/92‑1 (MQ=60)

         ACTTGTCCGTCAACCCAAACGGTATCTTTCCAGCCTCTGTCTTCCGGGAACGGCATCGCGCCATTCACGTTACGGATCTGGAACGTTACGCCTT                                                                                      >  SRR4302543.893696/1‑94 (MQ=60)

          CTTGTCCGTCAACCCAAACGGTATCTTTCCAGCCTCTGTCTTCCGGGAACGGCATCGCGCCATTCACGTTACGGATCTGGAACGTTACGCCTTC                                                                                     >  SRR4302543.1294994/1‑94 (MQ=60)

          CTTGTCCGTCAACCCAAACGGTATCTTTCCAGCCTCTGTCTTCCGGGAACGGCATCGCGCCATTCACGTTACGGATCTGGAACGTTACGCCTTC                                                                                     >  SRR4302543.1107244/1‑94 (MQ=60)

          CTTGTCCGTCAACCCAAACGGTATCTTTCCAGCCTCTGTCTTCCGGGAACGGCATCGCGCCATTCACGTTACGGATCTGGAACGTTACGCCTTC                                                                                     >  SRR4302543.6989299/1‑94 (MQ=60)

          CTTGTCCGTCAACCCAAACGGTATCTTTCCAGCCTCTGTCTTCCGGGAACGGCATCGCGCCATTCACGTTACGGATCTGGAACGTTACGCCTTC                                                                                     >  SRR4302543.5204862/1‑94 (MQ=60)

           TTGTCCGTCAACCCAAACGGTATCTTTCCAGCCTCTGTCTTCCGGGAACGGCATCGCGCCATTCACGTTACGGATCTGGAACGTTACGCCTTCA                                                                                    <  SRR4302543.7936774/94‑1 (MQ=60)

             GTCCGTCAACCCAAACGGTATCTTTCCAGCCTCTGTCTTCCGGGAACGGCATCGCGCCATTCACGTTACGGATCTGGAACGTTACGCCTTCAAT                                                                                  <  SRR4302543.3031347/94‑1 (MQ=60)

               CCGTCACCCCAAACGGTATCTTTCCAGCCTCTGTCTTCCGGGAACGGCATCGCGCCATTCACGTTACGGATCTGGAACGTTACGCCTTCAATAT                                                                                <  SRR4302543.4265201/94‑1 (MQ=60)

               CCGTCAACCCAAACGGTATCTTTCCAGCCTCTGTCTTCCGGGAACGGCATCGCGCCATTCACGTTACGGATCNGGAACGTTACGCCTTCAATAT                                                                                >  SRR4302543.1270338/1‑94 (MQ=60)

                CGTCAACCCAAACGGTATCTTTCCAGCCTCTGTCTTCCGGGAACGGCATCGCGCCATTCACGTTACGGATCTGGAACGTTACGCCTTCAATATG                                                                               <  SRR4302543.4962810/94‑1 (MQ=60)

                CGTCAACCCAAACGGTATCTTTCCAGCCTCTGTCTTCCGGGAACGGCATCGCGCCATTCACGTTACGGATCTGGAACGTTACGCCTTCAATATG                                                                               >  SRR4302543.7628538/1‑94 (MQ=60)

                CGTCAACCCAAACGGTATCTTTCCAGCCTCTGTCTTCCGGGAACGGCATCGCGCCATTCACGTTACGGATCTGGAACGTTACGCCTTCAATATG                                                                               <  SRR4302543.5938889/94‑1 (MQ=60)

                 GTCAACCCAAACGGTATCTTTCCAGCCTCTGTCTTCCGGGAACGGCATCGCGCCATTCACGTTACGGATCTGGAACGTTACGCCTTCAATATGG                                                                              >  SRR4302543.749605/1‑94 (MQ=60)

                 GTCAACCCAAACGGTATCTTTCCAGCCTCTGTCTTCCGGGAACGGCATCGCGCCATTCACGTTACGGATCTGGAACGTTACGCCTTCAATATGG                                                                              >  SRR4302543.1592983/1‑94 (MQ=60)

                 GTCAACCCAAACGGTATCTTTCCAGCCTCTGTCTTCCGGGAACGGCATCGCGCCATTCACGTTACGGATCTGGAACGTTACGCCTTCAATATGG                                                                              >  SRR4302543.5568186/1‑94 (MQ=60)

                   CAACCCAAACGGTATCTTTCCAGCCTCTGTCTTCCGGGAACGGCATCGCGCCATTCACGTTACGGATCTGGAACGTTACGCCTTCAATATGGAC                                                                            >  SRR4302543.2158847/1‑94 (MQ=60)

                   CAACCCAAACGGTATCTTTCCAGCCTCTGTCTTCCGGGAACGGCATCGCGCCATTCACGTTACGGATCTGGAACGTTACGCCTTCAATATGGAA                                                                            <  SRR4302543.1878960/94‑1 (MQ=60)

                    ACCCCAAACGGTATCTTTCCAGCCTCTGTCTTCCGGGAACGGCATCGCGCCATTCACGTTACGGATCTGGAACGTTACGCCTTCAATATGGAAC                                                                           <  SRR4302543.7855823/94‑1 (MQ=60)

                    AACCCAAACGGTATTTTTCCAGCCTCTGTCTTCCGGGAACGGCATCGCGCCATTCACGTTACGGATCTGGAACGTTACGCCTTCAATATGGAAC                                                                           <  SRR4302543.2635395/94‑1 (MQ=60)

                    AACCCAAACGGTATCTTTCCAGCCTCTGTCTTCCGGGAACGGCATCGCGCCATTCACGTTACGGATCTGGAACGTTACGCCTTCAATATGGAAC                                                                           <  SRR4302543.3240429/94‑1 (MQ=60)

                    AACCCAAACGGTATCTTTCCAGCCTCTGTCTTCCGGGAACGGCATCGCGCCATTCACGTTACGGATCTGGAACGTTACGCCTTCAATATGGAAC                                                                           <  SRR4302543.177399/94‑1 (MQ=60)

                    AACCCAAACGGTATCTTTCCAGCCTCTGTCTTCCGGGAACGGCATCGCGCCATTCACGTTACGGATCTGGAACGTTACGCCTTCAATATGGAAC                                                                           >  SRR4302543.1332369/1‑94 (MQ=60)

                     ACCCAACCGGTATCTTTCCAGCCTCTGTCTTCCGGGAACGGCATCGCGCCATTCTCGTTACGGATCTGGAACGTTACGCCTTCAATATGGAACG                                                                          <  SRR4302543.6811401/94‑1 (MQ=60)

                     ACCCAAACGGTATCTTTCCAGCCTCTGTCTTCCGGGAACGGCATCGCGCCATTCACGTTACGGATCTGGAACGTTACGCCTTCAATATGGAACG                                                                          <  SRR4302543.5208711/94‑1 (MQ=60)

                      CCCAAACGGTATCTTTCCAGCCTCTGTCTTCCGGGAACGGCATCGCGCCATTCACGTTACGGATCTGGAACGTTACGCCTTCAATATGGAACGC                                                                         >  SRR4302543.1191510/1‑94 (MQ=60)

                       CCAAACGGTATCTTTCCAGCCTCTGTCTTCCGGGAACGGCATCGCGCCATTCACGTTACGGATCTGGAACGTTACGCCTTCAATATGGAACGCT                                                                        <  SRR4302543.3360609/94‑1 (MQ=60)

                       CCAAACGGTATCTTTCCAGCCTCTGTCTTCCGGGAACGGCATCGCGCCATTCACGTTACGGATCTGGAACGTTACGCCTTCAATATGGAACGCT                                                                        >  SRR4302543.5028262/1‑94 (MQ=60)

                       CCAAACGGTATCTTTCCAGCCTCTGTCTTCCGGGAACGGCATCGCGCCATTCACGTTACGGATCTGGAACGTTACGCCTTCAATATGGAACGCT                                                                        >  SRR4302543.6057894/1‑94 (MQ=60)

                       CCAAACGGTATCTTTCCAGCCTCTGTCTTCCGGGAACGGCATCGCGCCATTCACGTTACGGATCTGGAACGTTACGCCTTCAATATGGAACGCT                                                                        <  SRR4302543.539657/94‑1 (MQ=60)

                       CCAAACGGTATCTTTCCAGCCTCTGTCTTCCGGGAACGGCATCGCGCCATTCACGTTACGGATCTGGAACGTTACGCCTTCAATATGGAACGCT                                                                        <  SRR4302543.539656/94‑1 (MQ=60)

                        CAAACGGTATCTTTCCAGCCTCTGTCTTCCGGGAACGGCATCGCGCCATTCACGTTACGGATCTGGAACGTTACGCCTTCAATATGGAACGCTT                                                                       <  SRR4302543.4908937/94‑1 (MQ=60)

                        CAAACGGTATCTTTCCAGCCTCTGTCTTCCGGGAACGGCATCGCGCCATTCACGTTACGGATCTGGAACGTTACGCCTTCAATATGGAACGCTT                                                                       >  SRR4302543.1831701/1‑94 (MQ=60)

                         AAACGGTATCTTTCCAGCCTCTGTCTTCCGGGAACGGCATCGCGCCATTCACGTTACGGATCTGGAACGTTACGCCTTCAATATGGAACGCTTG                                                                      >  SRR4302543.1326638/1‑94 (MQ=60)

                         AAACGGTATCTTTCCAGCCTCTGTCTTCCGGGAACGGCATCGCGCCATTCACGTTACGGATCTGGAACGTTACGCCTTCAATATGGAACGCTTG                                                                      >  SRR4302543.1095927/1‑94 (MQ=60)

                         AAACGGTATCTTTCCAGCCTCTGTCTTCCGGGAACGGCATCGCGCCATTCACGTTACGGATCTGGAACGTTACGCCTTCAATATGGAACGCTTG                                                                      >  SRR4302543.1641080/1‑94 (MQ=60)

                         AAACGGTATCTTTCCAGCCTCTGTCTTCCGGGAACGGCATCGCGCCATTCACGTTACGGATCTGGAACGTTACGCCTTCAATATGGAACGCTTG                                                                      >  SRR4302543.2199788/1‑94 (MQ=60)

                          AGCGGTATCTTTCCAGCCTCTGTCTTCCGGGAACGGCATCGCGCCATTCACGTTACGGATCTGGAACGTTACGCCTTCAATATGGAACGCTTGC                                                                     <  SRR4302543.7734830/94‑1 (MQ=60)

                           ACGGTATCTTTCCAGCCTCTGTCTTCCGGGAACGGCATCGCGCCATTCACGTTACGGATCTGGAACGTTACGCCTTCAATATGGAACGCTTGCG                                                                    >  SRR4302543.5786929/1‑94 (MQ=60)

                           ACGGTATCTTCCCAGCCTCTGTCTTCCGGGAACGGCATCGCGCCATTCACGTTACGGATCTGGAACGTTACGCCTTCAATATGGAACGCTTGCG                                                                    <  SRR4302543.488853/94‑1 (MQ=60)

                            CGGTATCTTTCCAGCCTCTGTCTTCCGGGAACGGCATCGCGCCATTCACGTTACGGATCTGGAACGTTACGCCTTCAATATGGAACGCTTGCGG                                                                   <  SRR4302543.900145/94‑1 (MQ=60)

                            CGGTATCTTTCCAGCCTCTGTCTTCCGGGAACGGCATCGCGCCATTCACGTTACGGATCTGGAACGTTACGCCTTCAATATGGAACGCTTGCGG                                                                   >  SRR4302543.5491464/1‑94 (MQ=60)

                            CGGTATCTTTCCAGCCTCTGTCTTCCGGGAACGGCATCGCGCCATTCACGTTACGGATCTGGAACGTTACGCCTTCAATATGGAACGCTTGCGG                                                                   >  SRR4302543.1075595/1‑94 (MQ=60)

                            CGGTATCTTTCCAGCCTCTGTCTTCCGGGAACGGCATCGCGCCATTCACGTTACGGATCTGGAACGTTACGCCTTCAATATGGAACGCTTGCGG                                                                   <  SRR4302543.5470476/94‑1 (MQ=60)

                             GGTATCTTTCCAGCCTCTGTCTTCCGGGAACGGCATCGCGCCATTCACGTTACGGATCTGGAACGTTACGCCTTCAATATGGAACGCTTGCGGC                                                                  >  SRR4302543.4084810/1‑94 (MQ=60)

                             GGTATCTTTCCAGCCTCTGTCTTCCGGGAACGGCATCGCGCCATTCACGTTACGGATCTGGAACGTTACGCCTTCAATATGGAACGCTTGCGGC                                                                  >  SRR4302543.7349406/1‑94 (MQ=60)

                              GTATCTTTCCAGCCTCTGTCTTCCGGGAACGGCATCGCGCCATTCACGTTACGGATCTGGAACGTTACGCCTTCAATATGGAACGCTTGCGGCT                                                                 >  SRR4302543.6103125/1‑94 (MQ=60)

                              GTATCTTTCCAGCCTCTGTCTTCCGGGAACGGCATCGCGCCATTCACGTTACGGATCTGGAACGTTACGCCTTCAATATGGAACGCTTGCGGCT                                                                 >  SRR4302543.5362828/1‑94 (MQ=60)

                                ATCTTTCCAGCCTCTGTCTTCCGGGAACGGCATCGCGCCATTCACGTTACGGATCTGGAACGTTACGCCTTCAATATGGAACGCTTGCGGCTCC                                                               >  SRR4302543.1161220/1‑94 (MQ=60)

                                  TTTTCCAGCCTCTGTCTTCCGGGAACGGCATCGCGCCATTCACGTTACGGATCTGGAACGTTACGCCTTCAATATGGAACGCTTGCGGCTCGTC                                                             <  SRR4302543.1143257/94‑1 (MQ=60)

                                  CTTTCCAGCCTCTGTCTTCCGGGAACGGCATCGCGCCATTCACGTTACGGATCTGGAACGTTACGCCTTCAATATGGAACGCTTGCGGCTCCTC                                                             >  SRR4302543.2937432/1‑94 (MQ=60)

                                   TTTCCAGCCTCTGTCTTCCGGGAACGGCATCGCGCCATTCACGTTACGGATCTGGAACGTTACGCCTTCAATATGGAACGCTTGCGGCTCGTTC                                                            >  SRR4302543.5916920/1‑94 (MQ=60)

                                   TTTCCAGCCTCTGTCTTCCGGGAACGGCATCGCGCCATTCACGTTACGGATCTGGAACGTTACGCCTTCAATATGGAACGCTTGCGGCTCGTCC                                                            <  SRR4302543.5690427/94‑1 (MQ=60)

                                   TTGCCAGCCTCTGTCTTCCGCGAACGGCATCGCACCTTTCACGTTACGGATCTGGAACATTACGCCTTCAATATGGAACGCTTGCGGCTCGTCC                                                            <  SRR4302543.5480808/94‑1 (MQ=60)

                                    TTCCCGCCTCAGTCTTCCGGGAACGGCATCACGCCATACACGTTACGGATCTAAAACGATACGCCTTCAAAATGGAACGCTTGCGGCTCGTCCG                                                           <  SRR4302543.7108186/94‑1 (MQ=53)

                                    TTCCAGCCTCTGTCTTCCGGGAACGGCATCGCGCCATTCACGTTACGGATCTGGAACGTTACGCCTTCAATATGGAACGCTTGCGGCTCccccc                                                           >  SRR4302543.6039542/1‑89 (MQ=60)

                                    TTCCAGCCTCTGTCTTCCGGGAACGGCATCGCGCCATTCACGTTACGGATCTGGAACGTTACGCCTTCAATATGGAACGCTTGCGGCTCccccc                                                           >  SRR4302543.1682134/1‑89 (MQ=60)

                                    TTCCAGCCTCTGTCTTCCGGGAACGGCATCGCGCCATTCACGTTACGGATCTGGAACGTTACGCCTTCAATATGGAACGCTTGCGGCTCGTCCG                                                           <  SRR4302543.7368988/94‑1 (MQ=60)

                                    TTCCAGCCTCTGTCTTCCGGGAACGGCATCGCGCCATTCACGTTACGGATCTGGAACGTTACGCCTTCAATATGGAACGCTTGCGGCTCGTCCC                                                           >  SRR4302543.2793545/1‑94 (MQ=60)

                                     TCCAGCCTCTGTCTTCCGGGAACGGCATCGCGCCATTCACGTTACGGATCTGGAACGTTACGCCTTCAATATGGAACGCTTGCGGCTCGTCCGC                                                          <  SRR4302543.5357865/94‑1 (MQ=60)

                                      CCAGCCTCTGTCTTCCGGGAACGGCATCGCGCCATTCACGTTACGGATCTGGAACGTTACGCCTTCAATATGGAACGCTTGCGGCTCccccccc                                                         >  SRR4302543.2939175/1‑87 (MQ=60)

                                      CCAGCCTCTGTCTTCCGGGAACGGCATCGCGCCATTCACGTTACGGATCTGGAACGTTACGCCTTCAATATGGAACGCTTGCGGCTCccccccc                                                         >  SRR4302543.4168450/1‑87 (MQ=60)

                                      CCAGCCTCTGTCTTCCGGGAACGGCATCGCGCCATTCACGTTACGGATCTGGAACGTTACGCCTTCAATATGGAACGCTTGCGGCTCCTCCGCG                                                         >  SRR4302543.7228662/1‑94 (MQ=60)

                                       CAGCCTCTGTCTTCCGGGAACGGCATCGCGCCATTCACGTTACGGATCTGGAACGTTACGCCTTCAATATGGAACGCTTGCGGCTCcgcccccc                                                        >  SRR4302543.7640191/1‑86 (MQ=60)

                                        AGCCTCTGTCTTCCGGGAACGGCATCGCGCCATTCACGTTACGGATCTGGAACGTTACGCCTTCAATATGGAACGCTTGCGGCTCCTCCCCGCG                                                       >  SRR4302543.3044657/1‑94 (MQ=60)

                                          CCTCTGTCTTCCGGGAACGGCATCGCGCCATTCACGTTACGGATCTGGAACGTTACGCCTTCAATATGGAACGCTTGCGGCTCCTCCGCGgggc                                                     >  SRR4302543.6321440/1‑90 (MQ=60)

                                           CTCTGTCTTCCGGGAACGGCATCGCGCCATTCACGTTACGGATCTGGAACGTTACGCCTTCAATATGGAACGCTTGCGGCTCccccccgccgac                                                    >  SRR4302543.3505644/1‑82 (MQ=60)

                                           CTCTGTCTTCCGGGAACGGCATCGCGCCATTCACGTTACGGATCTGGAACGTTACGCCTTCAATATGGAACGCTTGCGGCTCGTCCGCGCGTAC                                                    <  SRR4302543.4430883/94‑1 (MQ=60)

                                           CTCTGTCTTCCGGGAACGGCATCGCGCCATTCACGTTACGGATCTGGAACGTTACGCCTTCAATATGGAACGCTTGCGGCTCCCCCGCGCGGAC                                                    >  SRR4302543.3952313/1‑94 (MQ=60)

                                            TCTGTCTTCCGGGAACGGCATCGCGCCATTCACGTTACGGATCTGGAACGTTACGCCTTCAATATGGAACGCTTGCGGCTCGTCCGCGCGTACC                                                   <  SRR4302543.1722099/94‑1 (MQ=60)

                                             CTGTCTTCCGGGAACGGCATCGCGCCATTCACGTTACGGATCTGGAACGTTACGCCTTCAATATGGAACGCTTGCGGCTCccccccgcggaccg                                                  >  SRR4302543.4345922/1‑80 (MQ=60)

                                             CTGTCTTCCGGGAACGGCATCGCGCCATTCACGTTACGGATCTGGAACGTTACGCCTTCAATATGGAACGCTTGCGGCTCccccccgcgcaccc                                                  >  SRR4302543.2514512/1‑80 (MQ=60)

                                             CTGTCTTCCGGGAACGGCATCGCGCCATTCACGTTACGGATCTGGAACGTTACGCCTTCAATATGGAACGCTTGCGGCTCGTCCGCGCGTACCG                                                  <  SRR4302543.5901262/94‑1 (MQ=60)

                                              TGTCTTCCGGGAACGGCATCGCGCCATTCACGTTACGGATCTGGAACGTTACGCCTTCAATATGGAACGCTTGCGGCTCcccccccccggcccc                                                 >  SRR4302543.1902297/1‑79 (MQ=60)

                                              TGTCTTCCGGGAACGGCATCGCGCCATTCACGTTACGGATCTGGAACGTTACGCCTTCAATATGGAACGCTTGCGGCTCcccccccccggcccc                                                 >  SRR4302543.3279566/1‑79 (MQ=60)

                                               GTCTTCCGGGAACGGCATCGCGCCATTCACGTTACGGATCTGGAACGTTACGCCTTCAATATGGAACGCTTGCGGCTCccccccgcgcgccgcc                                                >  SRR4302543.1567276/1‑78 (MQ=60)

                                               GTCTTCCGGGAACGGCATCGCGCCATTCACGTTACGGATCTGGAACGTTACGCCTTCAATATGGAACGCTTGCGGCTCccccccccggcacgtc                                                >  SRR4302543.2486963/1‑78 (MQ=60)

                                               GTCTTCCGGGAACGGCATCGCGCCATTCACGTTACGGATCTGGAACGTTACGCCTTCAATATGGAACGCTTGCGGCTCcaccccccgcgcccgc                                                >  SRR4302543.3211102/1‑78 (MQ=60)

                                               GTCTTCCGGGAACGGCATCGCGCCATTCACGTTACGGATCTGGAACGTTACGCCTTCAATATGGAACGCTTGCGGCTCCCCCGCCCGCACCGTC                                                >  SRR4302543.1533443/1‑94 (MQ=60)

                                               GGCTTCCGGGAACGGCATCGCGCCATTCACGTTACGGATCTGGAACGTTACGCCTTCAATATGGAACGCTTGCGGCTCGTCCGCGCGTACCGTC                                                <  SRR4302543.4474779/94‑1 (MQ=60)

                                                TCTTCCGGGAACGGCATCGCGCCCTTCCCGTTACGGATCTGGAACGTTACGCCTTCAATATGGAACGCTTGCGGCTCctgctcgcgaacactcc                                               >  SRR4302543.434383/1‑77 (MQ=60)

                                                TCTTCCGGGAACGGCATCGCGCCATTCACGTTACGGATCTGGAACGTTACGCCTTCAATATGGAACGCTTGCGGCTCGTCCGCGCGTACCGTCC                                               <  SRR4302543.2190538/94‑1 (MQ=60)

                                                   TCCGGGAACGGCATCGCGCCATTCACGTTACGGATCTGGAACGTTACGCCTTCAATATGGAACGCTTGCGGCTCGTCCGCGCGTACCGTCCAGC                                            <  SRR4302543.6819687/94‑1 (MQ=60)

                                                    CCGGGAACGGCATCGCGCCATTCACGTTACGGATCTGGAACGTTACGCCTTCAATATGGAACGCTTGCGGCTCGTCCGCGCGTACCGTCCAGCG                                           <  SRR4302543.4201273/94‑1 (MQ=60)

                                                     CGGGAACGGCATCGCGCCATTCACGTTACGGATCTGGAACGTTACGCCTTCAATATGGAACGCTTGCGGCTCGTCCGCGCGTACCGTCCAGCGT                                          <  SRR4302543.2220558/94‑1 (MQ=60)

                                                      GGGAACGGCATCGCGCCATTCACGTTACGGATCTGGAACGTTACGCCTTCAATATGGAACGCTTGCGGCTCGTCCGCGCGcaccgccccgcggt                                         >  SRR4302543.6437303/1‑80 (MQ=60)

                                                       GGAACGGCATCGCGCCATTCACGTTACGGATCTGGAACGTTACGCCTTCAATATGGAACGCTTGCGGCTCGTCCGCGCGTACCGTCCAGCGTTC                                        <  SRR4302543.4592761/94‑1 (MQ=60)

                                                       GGAACGGCATCGCGCCATTCACGTTACGGATCTGGAACGTTACGCCTTCAATATGGAACGCTTGCGGCTCGTCCGCGCGTACCGTCCAGCGTTC                                        <  SRR4302543.1895932/94‑1 (MQ=60)

                                                       GGAACGGCATCGCGCCATTCACGTTACGGATCTGGAACGTTACGCCTTCAATATGGAACGCTTGCGGCTCCGCCGCGCGGGCCGTCCAGCGgtt                                        >  SRR4302543.3026492/1‑91 (MQ=60)

                                                        GAACGGCATCGCGCCATTCACGTTACGGATCTGGAACATTACGCCTTCAATATGGAACGCTTGCGGCTCctcccccccgaccctccagcggttc                                       >  SRR4302543.5523369/1‑69 (MQ=60)

                                                         AACGGCATCGCGCCATTCACGTTACGGATCTGGAACGTTACGCCTTCAATATGGAACGCTTGCGGCTCctccccgcggcacacccccgcgtccc                                      >  SRR4302543.6720004/1‑68 (MQ=60)

                                                         AACGGCATCGCGCCATTCACGTTACGGATCTGGAACGTTACGCCTTCAATATGGAACGCTTGCGGCTCGTCCGCGCGggcactcccgcggtgtc                                      >  SRR4302543.1747041/1‑77 (MQ=60)

                                                         AACGGCATCGCGCCATTCACGTTACGGATCTGGAACGTTACGCCTTCAATATGGAACGCTTGCGGCTCGTCCGCGCGTACCGTCCAGCGTTCCC                                      <  SRR4302543.7385286/94‑1 (MQ=60)

                                                          ACGGCATCGCGCCATTCACGTTACGGATCTGGAACGTTACGCCTTCAATATGGAACGCTTGCGGCTCGTCCGCGCGTACCGTCCAGCGTTCCCA                                     <  SRR4302543.2074636/94‑1 (MQ=60)

                                                           CGGCATCGCGCCATTCACGTTACGGATCTGGAACGTTACGCCTTCAATATGGAACGCTTGCGGCTCGTCCGCGCGTACCGTCCAGCGTTCCCAC                                    <  SRR4302543.3077231/94‑1 (MQ=60)

                                                            GGCATCGCGCCATTCACGTTACGGATCTGGAACGTTACGCCTTCAATATGGAACGCTTGCGGCTCGTCCGCGCGTACCGTCCAGCGTTCCCACG                                   <  SRR4302543.216419/94‑1 (MQ=60)

                                                              CATCGCGCCATTCACGTTACGGATCTGGAACGTTACGCCTTCAATATGGAACGCTTGCGGCTCccccgcgcggccccttccccgttccctccac                                 >  SRR4302543.2710653/1‑63 (MQ=60)

                                                              CATCGCGCCATTCACGTTACGGATCTGGAACGTTACGCCTTCAATATGGAACGCTTGCGGCTCCTCCGCGCGgtcccgcccacgctacccccgt                                 >  SRR4302543.4061359/1‑72 (MQ=60)

                                                               ATCGCGCCATTCACGTTACGGATCTGGAACGTTACGCCTTCAATATGGAACGCTTGCGGCTCcgccggcggggccgtgccgccgtcccgccgcc                                >  SRR4302543.6029789/1‑62 (MQ=60)

                                                               ATCGCGCCATTCACGTTACGGATCTGGAACGTTACGCCTTCAATATGGAACGCTTGCGGCTCGTCCGCGCCTACCGTCCCGCCTTCCCACcctc                                >  SRR4302543.7521774/1‑90 (MQ=60)

                                                                 CGCGCCATTCACGTTACGGATCTGGAACGTTACGCCTTCAATATGGAACGCTTGCGGCTCGTCCGCGCGTACCGTCCAGCGTTCCCACGTTCCT                              <  SRR4302543.5743198/94‑1 (MQ=60)

                                                                 CGCGCCATTCACGTTACGGATCTGGAACGTTACGCCTTCAATATGGAACGCTTGCGGCTCCTCCGCGCGTACCGTCCCGCGTTCCCcccctcct                              >  SRR4302543.1884250/1‑86 (MQ=60)

                                                                  GCGCCATTCACGTTACGGATCTGGAACGTTACGCCTTCAATATGGAACGCTTGCGGCTCGTCCGCGCGTACCGTCCAGCGTTCCCACGTTCCTT                             <  SRR4302543.366323/94‑1 (MQ=60)

                                                                  GCGCCATTCACGTTACGGATCTGGAACGTTACGCCTTCAATATGGAACGCTTGCGGCTCGTCCGCGCGTACCGTCCAGCGTTCCCACGTTCCTT                             <  SRR4302543.366322/94‑1 (MQ=60)

                                                                  GCGCCATTCACGTTACGGATCTGGAACGTTACGCCTTCAATATGGAACGCTTGCGGCTCGTCCGCGCGTACCGTCCAGCGTTCCCACGTTCCTT                             <  SRR4302543.7584520/94‑1 (MQ=60)

                                                                  GCGCCATTCACGTTACGGATCTGGAACGTTACGCCTTCAATATGGAACGCTTGCGGCTCGTCCGCGCGTACCGTCCAGCGTTCCCACGTTCCTT                             <  SRR4302543.3309565/94‑1 (MQ=60)

                                                                  GCGCCATTCACGTTACGGATCTGGAACGTTACGCCTTCAATATGGAACGCTTGCGGCTCGTCCGCGCGTACCGTCCAGCGTTCCCACGTTCCTT                             <  SRR4302543.1150352/94‑1 (MQ=60)

                                                                  GCGCCATTCACGTTACGGATCTGGAACGTTACGCCTTCAATATGGAACGCTTGCGGCTCGTCCGCGCGTACCGTCCAGCGTTCCCACGTTCCTT                             <  SRR4302543.6878271/94‑1 (MQ=60)

                                                                  GCGCCATTCACGTTACGGATCTGGAACGTTACGCCTTCAATATGGAACGCTTGCGGCTCGCCCGCGCGgcacgtccccgcttccccccctcctt                             >  SRR4302543.6451754/1‑68 (MQ=60)

                                                                   CGCCATTCACGTTACGGATCTGGAACGTTACGCCTTCAATATGGAACGCTTGCGGCTCGTCCGCGCGTACCGTCCAGCGTTCCCACGTTCCTTG                            <  SRR4302543.3494943/94‑1 (MQ=60)

                                                                   CGCCATTCACGTTACGGATCTGGAACGTTACGCCTTCAATATGGAACGCTTGCGGCTCCTCCGCGCGTcgcggccccgctgtcccccttccctt                            >  SRR4302543.3129553/1‑68 (MQ=60)

                                                                     CCATTCACGTTACGGATCTGGAACGTTACGCCTTCAATATGGAACGCTTGCGGCTCCCCCGCGCGTACCcgccagcggtcccccccccctctct                          >  SRR4302543.791935/1‑69 (MQ=60)

                                                                     CCATTCACGTTACGGATCTGGAACGTTACGCCTTCAATATGGAACGCTCGCGGCTCcctcccgcggaccgttcctcgttctccccctcccttcc                          >  SRR4302543.7106575/1‑56 (MQ=60)

                                                                      CATTCACGTTACGGATCTGGAACGTTACGCCTTCAATATGGAACGCTTGCGGCTCGTtcgcccctatcggtccaaggtcccgcgcttctttctc                         >  SRR4302543.6370927/1‑57 (MQ=60)

                                                                      CATTCACGTTACGGATCTGGAACGTTACGCCTTCAATATGGAACGCTTGCGGCTCCCCCCCGCGGACCGTCCAGCGTTCCCACCTTCCTctctg                         >  SRR4302543.3803704/1‑89 (MQ=60)

                                                                       ATTCACGTTACGGATCTGGAACGTTACGCCTTCAATATGGAACGCTTGCGGCTCctccccccggaccgtcccgccttccccccctccttgctgc                        >  SRR4302543.5642304/1‑54 (MQ=60)

                                                                       ATTCACGTTACGGATCTGGAACGTTACGCCTTCAATATGGAACGCTTGCGGCTCGTCCGCGCGTACCGTCCAccggttccacctttccttctgc                        >  SRR4302543.4733820/1‑72 (MQ=60)

                                                                       ATTCACGTTACGGATCTGGAACGTTACGCCTTCAATATGGAACGCTTGCGGCTCGTCCCCGCGTACCGTCCAGCGgttcccccctcctttctgc                        >  SRR4302543.2841227/1‑75 (MQ=60)

                                                                       ATTCACGTTACGGATCTGGAACGTTACGCCTTCAATATGGAACGCTTGCGGCTCCTCCGCGCGTACCGTCCCGCGTTCCCcccctccttgcttc                        >  SRR4302543.6571809/1‑80 (MQ=60)

                                                                       ATTCACGTTACGGATCTGGAACGTTACGCCTTCAATATGGAACGCTTGCGGCTCCTCCGCGCGGACCGTCCACCGGTCCCCCGTTCCTTGCTGC                        >  SRR4302543.1737607/1‑94 (MQ=60)

                                                                       ATTCACGTTACGGATCTGGAACGTTACGCCTTCAATATGGAACGCTTGCGGCTCCTCCCCGCGGACCGTCCACCGTTCCCccctttctttctgc                        >  SRR4302543.7288314/1‑80 (MQ=60)

                                                                        TTCACGTTACGGAGCTGGAACGTTACGCCTTCAATATGGAACGCTTGCGGCTCGGCCGCGCGTACCGTCCAGCGTTCCCACGTTCCTTGCTGCG                       <  SRR4302543.6442530/94‑1 (MQ=60)

                                                                         TCACGTTACGGATCTGGAACGTTACGCCTTCAATATGGAACGCTTGCGGCTCctgcccgtgagcgcgccgccggtccgccagtcctttctcgac                      >  SRR4302543.6178896/1‑52 (MQ=60)

                                                                            CGTTACGGATCTGGAACGTTACGCCTTCAATATGGAACGCTTGCGGCTCGTCCGCGCGTACCGTCCAGCGTTCCCACGTTCCTTGCTGCGCGGT                   <  SRR4302543.5208872/94‑1 (MQ=60)

                                                                            CGTTACGGATCTGGAACGTTACGCCTTCAATATGGAACGCTTGCGGCTCGGCCCCGCGTACCGTCCAGCGgtcccccctttctttctgcgcggg                   >  SRR4302543.4517302/1‑70 (MQ=60)

                                                                             GTTACGGATCTGGACCGTTCCGCCTTCAATATGAAACGCTTGCGGCTCGTCCGCGCGTACCGTCCAGCGTTCCCACGTTCCTTGCTGCGCGGTG                  <  SRR4302543.7723630/94‑1 (MQ=60)

                                                                             GTTACGGATCTGGAACGTTACGCCTTCAATATGGAACGCTTGCGGCTCccccccgcggaccgtcccgcgtttccccgctcctttcttcgccgcg                  >  SRR4302543.5381400/1‑48 (MQ=60)

                                                                              TTACGGATCTGGAACGTTACGCCTTCAATATGGAACGCTTGagactcgtcgcccggcgccgctgcaagtttgcacgcttccttcggccgggggc                 >  SRR4302543.7213403/1‑41 (MQ=60)

                                                                              TTACGGATCTGGAACGTTACGCCTTCAATATGGAACGCTTGCGGCTCGTCCGCGCGTACCGTCCAGCGTTCCCACGTTCCTTGCTGCGCGGTGA                 <  SRR4302543.5847215/94‑1 (MQ=60)

                                                                              GTACGGATCTGGAACGTTACGCCTTCAATATGGAACGCTTGCGGCTCGTCCGCGCGTACCGTCCAGCGTTCCCACGTTCCTTGCTGCGCGGTGA                 <  SRR4302543.1759178/94‑1 (MQ=60)

                                                                               TACGGATCTGGAACGTTACGCCTTCAATATGGAACGCTTGCGGCTCGTCCGCGCGTACCGTCCAGCGTTCCCACGTTCCTTGCTGCGCGGTGAC                <  SRR4302543.2880243/94‑1 (MQ=60)

                                                                               TACGGATCTGGAACGTTACGCCTTCAATATGGAACGCTTGCGGCTCGTCCGCGCGTACCGTCCAGCGTTCCCACGTTCCTTGCTGCGCGGTGAC                <  SRR4302543.156367/94‑1 (MQ=60)

                                                                               TACGGATCTGGAACGTTACGCCTTCAATATGGAACGCTTGCGGCTCCTCCGCGCGGACCGTCCCGCGGTCCCCCCCTCCTTTCTGCGCGGTGAC                >  SRR4302543.2230594/1‑94 (MQ=53)

                                                                                TCGGATCTGGAACGTTACGCCTTCAATATGGAACGCTTGCGGCTCGTCCGCGCGTACCGTCCAGCGTTCCCACGTCCCTTGCTGCGCGGTGACA               <  SRR4302543.3063386/94‑1 (MQ=60)

                                                                                ACGGATCTGGAACGTTACGCCTTCAATATGGAACGCTTGCGGCTCGTCCGCGCGTACCGTCCAGCGTTCCCACGTTCCTTGCTGCGCGGTGACA               <  SRR4302543.4202240/94‑1 (MQ=60)

                                                                                ACGGATCTGGAACGTTACGCCTTCAATATGGAACGCTTGCGGCTCGTCCGCGCGTACCGTCCAGCGTTCCCACGTTCCTTGCTGCGCGGTGACA               <  SRR4302543.80295/94‑1 (MQ=60)

                                                                                 CGGATCTGGAACGTTACGCCTTCAATATGGAACGCTTGCGGCTCGgccccgcgcaaccttccgcgttccccccatccctttcgcgcggtgtcat              >  SRR4302543.5727864/1‑45 (MQ=60)

                                                                                 CGGATCTGGAACGTTACGCCTTCAATATGGAACGCTTGCGGCTCGTCCGCGCGTACCGTCCAGCGTTCCCACGTTCCTTGCTGCGCGGTGACAT              <  SRR4302543.6300598/94‑1 (MQ=60)

                                                                                  GGATCTGGAACGTTACGCCTTCAATATGGAACGCTTGCGGCTCGTCCGCGCGTACCGTCCAGCGTTCCCACGTTCCTTGCTGCGCGGTGACATC             <  SRR4302543.1037501/94‑1 (MQ=60)

                                                                                   GATCTGGAACGTTACGCCTTCAATATGGAACGCTTGCGGCTCGTCCGCGCGTACCGTCCAGCGTTCCCACGTTCCTTGCTGCGCGGTGACATCA            <  SRR4302543.4897961/94‑1 (MQ=60)

                                                                                     TCTGGAACGTTACGCCTTCAATATGGAACGCTTGCGGCTCGTCCGCGCGTACCGTCCAGCGTTCCCACGTTCCTTGCTGCGCGGTGACATCAAT          <  SRR4302543.3198324/94‑1 (MQ=60)

                                                                                     ACTGGAACGTTACGCCGTCAATATGGAACGCTTGCGGCTCGTCCGCGCGTACCGTCCAGCGTTCCCACGTTCCTTGCTGCGCGGTGACATCAAT          <  SRR4302543.3198323/94‑1 (MQ=60)

                                                                                      CTGGAACGTTACGCCTTCAATATGGAACGCTTGCGGCTCGTCCGCTCGTACCGTCCAGCGTTCCCACGTTCCTTGCTGCGCGGTGACATCAATA         <  SRR4302543.1000580/94‑1 (MQ=60)

                                                                                       TGGAACGTTACGCCTTCAATATGGAACGCTTGCGGCTCccccgcggcgaccgcgctagctgtcccccgttcctggttcgtcgggtactcaatac        >  SRR4302543.3534407/1‑38 (MQ=60)

                                                                                       TGGAACGTTACGCCTTCAATATGGAACGCTTGCGGCTCccccccgcggacccgccagcgggcccccggtccttgcctcgcggggacatacataa        >  SRR4302543.5269303/1‑38 (MQ=60)

                                                                                       TGGAACGTTACGCCTTCAATATGGAACGCTTGCGGCTCGTCCGCGCGTACCGTCCAGCGTTCCCACGTTCCTTGCTGCGCGGTGACATCAATAC        <  SRR4302543.340421/94‑1 (MQ=60)

                                                                                       TGGAACGTTACGCCTTCAATATGGAACGCTTGCGGCTCGTCCGCGCGTACCGTCCAGCGTTCCCACGTTCCTTGCTGCGCGGTGACATCAATAC        <  SRR4302543.4923206/94‑1 (MQ=60)

                                                                                       TGGAACGTTACGCCTTCAATATGGAACGCTTGCGGCTCGTCCGCGCGTACCGTCCAGCGTTCCCACGTTCCTTGCTGCGCGGTGACATCAATAC        <  SRR4302543.5013052/94‑1 (MQ=60)

                                                                                       TGGAACGTTACGCCTTCAATATGGAACGCTTGCGGCTCGTCCGCCCCTACCGTCCAcagttgccccgccccttcttgggcggtggcaccaattc        >  SRR4302543.7416121/1‑56 (MQ=59)

                                                                                       TGGAACGTTACGCCTTCAATATGGAACGCTTGCGGCTCCCCCGCGCGTACCGTCCAGCGTTCCCCCGTTCCTTGCTGCGCGGTGACATCAATAC        >  SRR4302543.7983749/1‑94 (MQ=60)

                                                                                        GGAACGTTACGCCTTCAATATGGAACGCTTGCGGCTCGTCCGCGCGTACCGTCCAGCGTTCCCACGTTCCTTGCTGCGCGGTGACATCAATACG       <  SRR4302543.6715360/94‑1 (MQ=60)

                                                                                        GGAACGTTACGCCTTCAATATGGAACGCTTGCGGCTCGTCCGCGCGTACCGTCCAGCGTTCCCACGTTCCTTGCTGCGCGGTGACATCAATACG       <  SRR4302543.755899/94‑1 (MQ=60)

                                                                                        GGAACGTTACGCCTTCAATATGGAACGCTTGCGGCTCGTCCGCGCGTACCGTCCAGCGTTCCCACGTTCCTTGCTGCGCGGTGACATCAATACG       <  SRR4302543.1250932/94‑1 (MQ=60)

                                                                                        GGAACGTTACGCCTTCAATATGGAACGCTTGCGGCTCGTCCGCGCGTACCGTCCAGCGTTCCCACGTTCCTTGCTGCGCGGTGACATCAATACG       <  SRR4302543.2232771/94‑1 (MQ=60)

                                                                                        GGAACGTTACGCCTTCAATATGGAACGCTTGCGGCTCCTCCGCCCGGACCCTCCAGCGGTCCCACGCTCCTTtcttcgcgggggcaacaaaaag       >  SRR4302543.3022282/1‑72 (MQ=35)

                                                                                        CGAACGTTACGCCTTCAATATGGAACGCTTGCGGCTCGTCCGCGCGTACCGTCCAGCGTTCCCACGTTCCTTGCTGCGCGGTGACATCAATACG       <  SRR4302543.3360532/94‑1 (MQ=60)

                                                                                         GAACGTTACGCCTTCAATATGGAACGCTTGCGGCTCGTCCGCGCGTACCGTCCAGCGTTCCCACGTTCCTTGCTGCGCGGTGACATCAATACGG      <  SRR4302543.6765081/94‑1 (MQ=60)

                                                                                         GAACGTTACGCCTTCAATATGGAACGCTTGCGGCTCGTCCGCGCGTACCGTCCAGCGTTCCCACGTTCCTTGCTGCGCGGTGACATCAATACGG      <  SRR4302543.5353638/94‑1 (MQ=60)

                                                                                          AACGTTACGCCTTCAATATGGAACGCTTGCGGCTCGTCCGCGCGTACCGTCCAGCGTTCCCACGTTCCTTGCTGCGCGGTGACATCAATACGGT     <  SRR4302543.1838498/94‑1 (MQ=60)

                                                                                          AACGTTACGCCTTCAATATGGAACGCTTGCGGCTCGTCCGCGCGTACCGTCCAGCGTTCCCACGTTCCTTGCTGCGCGGTGACATCAATACGGT     <  SRR4302543.1618429/94‑1 (MQ=60)

                                                                                          AACGTTACGCCTTCAATATGGAACGCTTGCGGCTCGTCCGCGCGTACCGTCCAGCGTTCCCACGTTCCTTGCTGCGCGGTGACATCAATACGGT     <  SRR4302543.1109311/94‑1 (MQ=60)

                                                                                          AACGTTACGCCTTCAATATGGAACGCTTGCGGCTCCTCCGCCCCGACCCTCCAGCGTTCCCCCCCTCCTTGCTGCGCGGGGACATacaaaacgt     >  SRR4302543.6173751/1‑85 (MQ=29)

                                                                                          AACGTTACGCCTTCAATATGGAACGCTTGCGGCTCCTCCCCGCGTACCGTCCAGCGTTTCCACGCTCCTTTCTGCGCGGGGACATCAATACGGT     >  SRR4302543.4554296/1‑94 (MQ=60)

                                                                                            CGTTACGCCTTCAATATGGAACGCTTGCGGCTCGTCCGCGCGTACCGTCCAGCGTTCCCACGTTCCTTGCTGCGCGGTGACATCAATACGGTTG   <  SRR4302543.1626910/94‑1 (MQ=60)

                                                                                            CGTTACGCCTTCAATATGGAACGCTTGCGGCTCGTCCGCGCGTACCGTCCAGCGTTCCCACGTTCCTTGCTGCGCGGTGACATCAATACGGTTG   <  SRR4302543.5004930/94‑1 (MQ=60)

                                                                                            CGTTACGCCTTCAATATGGAACGCTTGCGGCTCGTCCCCGCGGACCGTCCCGCGTTCCCcccttcccttctgcgcggggacaccaatacagttt   >  SRR4302543.1430625/1‑59 (MQ=59)

                                                                                            CATTACGCCTTCAATATGGAACGCTTGCGGCTCGgccccgcgttcctcccacggttcacccggcccatgctgcgccgtggaatcaacaacgttg   >  SRR4302543.4699063/1‑34 (MQ=60)

                                                                                             GTTACGCCTTTAATATGGAACGCTTGCGGCTCGTCCGCGCGTACCGTCCAGCGTTCCCACGTTCCTTGCTGCGCGGTGACATCAATACGGTTGA  <  SRR4302543.3810560/94‑1 (MQ=60)

                                                                                             GTTACGCCTTCAATATGGAACGCTTGCGGCTCCTCCGCCCGTCCCGTCCcgcgctcccaacctccctgcctctcggtgaccttcatacagtttc  >  SRR4302543.3759662/1‑49 (MQ=60)

                                                                                             GTTACGCCTTCAATATGGAACGCTTGCGGCTCCTCCCCGCGTACCGTCCAGCGTTCCCCCGTTCCTTGCTGCGCGGGGACATCAATACGGTTGA  >  SRR4302543.4012997/1‑94 (MQ=60)

AGCAGCTCCACTTGTCCGTCAACCCAAACGGTATCTTTCCAGCCTCTGTCTTCCGGGAACGGCATCGCGCCATTCACGTTACGGATCTGGAACATTACGCCTTCAATATGGAACGCTTGCGGCTCGTCCGCGCGTACCGTCCAGCGTTCCCACGTTCCTTGCTGCGCGGTGACATCAATACGGTTGA  >  NC_000913/3159388‑3159574

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 2 ≤` `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`