Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I1 R1	1696	279.3	15874187	97.8%	15524954	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,175,525	(C)_6→7	coding (402/630 nt)	yqiE ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,175,519	1	.	C	96.6%	724.6 / 16.2	238	coding (408/630 nt)	yqiE	hypothetical protein
Reads supporting (aligned to +/- strand): ref base . (5/3); new base C (133/97); total (138/100)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.67e-01

Only 100 of 290 total aligned reads displayed.

CGAATATCTTCGTTTTCATCAGCCAGACCGTGAATACCGCTTGCGGTCGTGGCGTCCACTTCGCCCACCATAATTGACGAACGCTCACTGGT‑G‑‑CCCCCC‑GGGCTTGCCAGGAAACTTAACACCGGTTTGGTCCGTTTGACTATCAGTCCCGCCTCTTCAATCGCTTCGCGACGGGCAACA  >  NC_000913/3175427‑3175606

 gAATATCTTCGTTTTCATCAGCCAGACCGTGAATACCGCTTGCGGTCGTGGCGTCCACTTCGCCCACCATAATTGACGAACGCTCACTGGT‑G‑‑c                                                                                         <  1:451625/93‑1 (MQ=255)

 gAATATCTTCGTTTTCATCAGCCAGACCGTGAATACCGCTTGCGGTCGTGGCGTCCACTTCGCCCACCATAATTGACGAACGCTCACTGGT‑G‑‑c                                                                                         >  1:7304437/1‑93 (MQ=255)

  aaTATCTTCGTTTTCATCAGCCAGACCGTGAATACCGCTTGCGGTCGTGGCGTCCATTTCGCCCACCATAATTGACGAACGCTCACTGGT‑G‑‑cc                                                                                        <  1:3900076/93‑1 (MQ=255)

   atatCTTCTTTTTCATCAGCCAGCCCGTGAATACCGCTTGCGGTCGTGGCGTCCACTTCGCCCACCATAATTGACGAACGCTCACTGGT‑G‑‑ccc                                                                                       <  2:5736007/93‑1 (MQ=255)

    tatCTTCGTTTTCATCAGCCAGACCGTGAATACCGCTTGCGGTCGTGGCGTCCACTTCGCCCACCATAATTGACGAACGCTCACTGGT‑G‑‑cccc                                                                                      >  1:5891315/1‑93 (MQ=255)

     atCTTCGTTTTCATCAGCCAGACCGTGAATACCGCTTGCGGTCGTGGCGTCCACTTCGCCCACCATAATTGACGAACGCTCACTGGT‑G‑‑ccccc                                                                                     >  2:528629/1‑93 (MQ=255)

     atCTTCGTTTTCATCAGCCAGACCGTGAATACCGCTTGCGGTCGTGGCGTCCACTTCGCCCACCATAATTGACGAACGCTCACTGGT‑G‑‑ccccc                                                                                     >  2:7344907/1‑93 (MQ=255)

      tCTTCGTTTTCATCAGCCAGACCGTGAATACCGCTTGCGGTCGTGGCGTCCACTTCGCCCACCATAATTGACGAACGCTCACTGGT‑G‑‑cccccc                                                                                    >  2:2036651/1‑93 (MQ=255)

      tCTTCGTTTTCATCAGCCAGACCGTGAATACCGCTTGCGGTCGTGGCGTCCACTTCGCCCACCATAATTGACGAACGCTCACTGGT‑G‑‑cccccc                                                                                    >  1:2121954/1‑93 (MQ=255)

      tCTTCGTTTTCATCAGCCAGACCGTGAATACCGCTTGCGGTCGTGGCGTCCACTTCGCCCACCATAATTGACGAACGCTCACTGGG‑G‑‑cccccc                                                                                    >  1:6718535/1‑93 (MQ=255)

        ttCTTTTTCATCAGCCAGACCGTGAATCCCGCTGGCGGTCGTGGCGTCCACTTCGCCCACCATAATTGACGAACGCTCACTGGT‑G‑‑cccccccg                                                                                  <  1:1556360/93‑3 (MQ=255)

        ttCGTTTTCATCAGCCAGACCGTGAATACCGCTTGCGGTCGTGGCGTCCACTTCGCCCACCATAATTGACGAACGCTCACTGGT‑G‑‑cccccccg                                                                                  >  1:7042018/1‑91 (MQ=255)

             tttCATCAGCCAGACCGTGAATACCGCTTGCGGTCGTGGCGTCCACTTCGCCCACCATAATTGACGAACGCTCACTGGT‑GC‑CCCCCC‑GGGCtt                                                                             >  1:3646459/1‑93 (MQ=255)

               tcatcaCCCAGGCCGTGAATCCCGCTTGCGGTCGTGGCGCCCACTTCGCCCACCATAATTGACGAACGCTCACTGGT‑GC‑CCCCCC‑GGGCTTGc                                                                           <  2:3109070/93‑1 (MQ=255)

                  tcaGCCAGACCGTGAATACCGCTTGCGGTCGTGGCGTCCACTTCGCCCACCATAATTGACGAACGCTCACTGGT‑GC‑CCCCCC‑GGGCTTGCCAg                                                                        >  1:4551044/1‑93 (MQ=255)

                    aGCCAGACCGTGAATACCGCTTGCGGTCGTGGCGTCCACTTCGCCCACCATAATTGACGAACGCTCACTGGT‑GC‑CCCCCC‑GGGCTTGCCAGGa                                                                      >  2:1780060/1‑93 (MQ=255)

                     gCCAGACCGTGAATACCGCTTGCGGTCGTGGCGTCCACTTCGCCCACCATAATTGACGAACGCTCACTGGT‑GC‑CCCCCC‑GGGCTTGCCGGGaa                                                                     >  2:1442823/1‑93 (MQ=255)

                     gCCAGACCGTGAATACCGCTTGCGGTCGTGGCGTCCACTTCGCCCACCATAATTGACGAACGCTCACTGGT‑GC‑CCCCCC‑GGGCTTGCCAGGaa                                                                     >  2:3205241/1‑93 (MQ=255)

                     gCCAGACCGTGAATACCGCTTGCGGTCGTGGCGTCCACTTCGCCCACCATAATTGACGAACGCTCACTGGT‑GC‑CCCCCC‑GGGCTTGCCAGGaa                                                                     >  1:4372362/1‑93 (MQ=255)

                      ccAGACCGTGAATACCGCTTGCGGTCGTGGCGTCCACTTCGCCCACCATAATTGACGAACGCTCACTGGT‑GC‑CCCCCC‑GGGCTTGCCAGGaaa                                                                    <  1:4294057/93‑1 (MQ=255)

                       cAGACGGTGAATACCGATTGGGGTCGTGGCATCCACTTCGCCCACCATAATTGACGAACGCTCACTGGT‑GC‑CCCCCC‑GGGCTTGCCAGGAAAc                                                                   <  2:4437948/93‑1 (MQ=255)

                       cAGACCGTGAATACCGCTTGCGGTCGTGGCGTCCACTTCGCCCACCATAATTGACGAACGCTCACTGGT‑GCCCCCCCC‑GGGCTTGCCAGGaaa                                                                    >  1:5011711/1‑93 (MQ=255)

                       cAGACCGTGAATACCGCTTGCGGTCGTGGCGTCCACTTCGCCCACCATAATTGACGAACGCTCACTGGT‑GC‑CCCCCC‑GGGCTTGCCAGGAAAc                                                                   >  2:1719887/1‑93 (MQ=255)

                        aGACCGTGAATACCGCTTGCGGTCGTGGCGTCCACTTCGCCCACCATAATTGACGAACGCTCACTGGT‑GC‑CCCCCC‑GGGCTTGCCAGGAAACt                                                                  >  2:3492329/1‑93 (MQ=255)

                        aGACCGTGAATACCGCTTGCGGCCGTGGCGTCCACTTCGCTCACTCTAATTAACGAACGCTCACTGGG‑GT‑CCCCCC‑GGGCTTGCCAGGACACt                                                                  >  1:2404271/1‑93 (MQ=255)

                           ccGTGAATCCCGCTTGCGTTCGTGCCGTCCACTTCCCCCACCATAATTGACGAACGCTCACTGGT‑GC‑CCCCCC‑GGGCTTGCCAGGAAACTTaa                                                               <  1:5963308/93‑1 (MQ=255)

                           ccGTGAATACCGCTTGCGGTCGTGGCGTCCACTTCGCCCACCATAATTGACGAACGCTCACTGGT‑GC‑CCCCCC‑GGGCTTGCCAGGAAACTTaa                                                               >  1:4659853/1‑93 (MQ=255)

                             gTGATTCCCGCTGGCGGAGGTGGCGCCCACTTCGCCCACCATAATTGACGAACGCTCACTGGT‑GC‑CCCCCC‑GGGCTTGCCAGGAAACTTAaca                                                             <  2:5140636/93‑1 (MQ=255)

                             gTGAATACCGCTTGCGGTCGTGGCGTCCACTTCGCCCACCATAATTGACGAACGCTCACTGGT‑GCCCCCCCC‑GGGCTTGCCAGGAAACTTAac                                                              >  2:1346595/1‑93 (MQ=255)

                             gTGAATACCGCTTGCGGTCGTGGCGTCCACTTCGCCCACCATAATTGACGAACGCTCACTGGT‑GC‑CCCCCC‑GGGCTTGCCAGGAAACTTAaca                                                             >  2:2305459/1‑93 (MQ=255)

                              tGAATCCCGCTTGCGGTCGTGCCGTCCACTTCGCCCACCATAATTGACGAACGCTCACTGGT‑GCCCCCCCC‑GGGCTTGCCAGGAAACTTAaca                                                             <  1:1182375/93‑1 (MQ=255)

                               gAATACCGCTTGCGGTCGTGGCGTCCACTTCGCCCACCATAATTGACGAACGCTCACTGGT‑GC‑CCCCCC‑GGGCTGGCCAGTAAACTTAACGcc                                                           >  1:1122490/1‑93 (MQ=255)

                                 atcccGCTTGCGGTCGTGGCGTCCCCTTCGCCCACCATAATTGACGAACGCTCACTGGT‑GC‑CCCCCC‑GGGCTTGCCAGGAAACTTAACACCgg                                                         <  2:6400213/90‑1 (MQ=255)

                                 aTACCGCTTGCGGTCGTGGCGTCCACTTCGCCCACCATAATTGACGAACGCTCACTGGT‑GC‑CCCCCC‑GGGCTTGCCAGGAAACTTAACACCgg                                                         >  1:1691110/1‑93 (MQ=255)

                                   cccccTTGCGGTCGTGTCGCCCACTTCGCCCACCATAATTGACGAACGCTCACTGGT‑GC‑CCCCCC‑GGGCTTGCCAGGAAACTTAACACCGGtt                                                       <  1:4801911/89‑1 (MQ=255)

                                   cccGCTTGGGGTCGTGGCGTCCACTTCGCCCACCATAATTGACGAACGCTCACTGGT‑GC‑CCCCCC‑GGGCTTGCCAGGAAACTTAACACCGGtt                                                       <  2:2575056/92‑1 (MQ=255)

                                   aCCGCTTGCGGTCGTGGCGTCCACTTCGCCCACCATAATTGACGAACGCTCACTGGT‑GC‑CCCCCC‑GGGCTTGCCAGGAAACTTAACACCGGtt                                                       >  1:7734941/1‑93 (MQ=255)

                                    ccGCTTGCGGTCGTGGCGTCCCCTTCGCCCACCATAATTGACGAACGCTCACTGGT‑GC‑CCCCCC‑GGGCTTGCCAGGAAACTTAACACCGGttt                                                      <  1:3549400/93‑1 (MQ=255)

                                    ccGCTTGCGGTCGTGGCGTCCACTTCGCCCACCATAATTGACGAACGCTCACTGGT‑GC‑CCCCCC‑GGGCTTGCCAGGAAACTTAACACCGGttt                                                      <  1:2746735/93‑1 (MQ=255)

                                     cGCTTGCGGTGGGGGCTCCCCCTGCGCCCACCATAATTGACGAACGCTCACTGGT‑GC‑CCCCCC‑GGGCTTGCCAGGAAACTTAACACCGGTTTg                                                     <  1:3360525/93‑1 (MQ=255)

                                     cGCTTGCGGTCGTGGCGTCCACTTCGCCCACCATAATTGACGAACGCTCACTGGT‑GCCCCCCCC‑GGGCTTGCCAGGAAACTTAACACCGGttt                                                      >  1:5264413/1‑93 (MQ=255)

                                     cGCTTGCGGTCGTGGCGTCCACTTCGCCCACCATAATTGACGAACGCTCACTGGT‑GC‑CCCCCC‑GGGCTTGCCAGGAAACTTAACACCGGTTTg                                                     >  2:4936881/1‑93 (MQ=255)

                                      gCTTGCGGTCGTGGCGTCCACTTCGCCCACCATAATTGACGAACGCTCACTGGT‑GC‑CCCCCC‑GGGCTTGCCAGGAAACTTAACACCGGTTTgg                                                    >  2:7544975/1‑93 (MQ=255)

                                      gCTTGCGGTCGGGGCGTCCACTTCGCCCACCATAATTGACGAACGCTCACTGGT‑GC‑CCCCCC‑GGGCTTGCCAGGAAACTTAACACCGGTTTgg                                                    <  2:2436323/93‑1 (MQ=255)

                                      ccTTGCGGTGGTGGCGTCCACTTCGCCCACCATAATTGACGAACGCTCACTGGT‑GC‑CCCCCC‑GGGCTTGCCAGGAAACTTAACACCGGTTTgg                                                    <  1:1768701/92‑1 (MQ=255)

                                       cTTGCGGTGGTGGCTTCCACTCCGCCCACCATAATTGACGAACGCTCACTGGT‑GC‑CCCCCC‑GGGCTTGCCAGGAAACTTAACACCGGTTTGGt                                                   <  2:4769874/93‑1 (MQ=255)

                                       cTTGCGGTCGTGGCGTCCACTTCGCCCACCATAATTGACGAACGCTCACTGGT‑GC‑CCCCCC‑GGGCTTGCTAGGAAACTTAACACCGGTTTGGt                                                   >  1:985842/1‑93 (MQ=255)

                                       cTTGCGGTCGTGGCGTCCACTTCGCCCACCATAATTGACGAACGCTCACTGGT‑GC‑CCCCCC‑GGGCTTGCCAGGAAACTTAACACCGGTTTGGt                                                   >  1:7109735/1‑93 (MQ=255)

                                       cTTGCGGTCGTGGCGTCCACTTCGCCCACCATAATTGACGAACGCTCACTGGT‑GC‑CCCCCC‑GGGCTTGCCAGGAAACTTAACACCGGTTTGGt                                                   >  2:4259733/1‑93 (MQ=255)

                                        ttGCGGTCGTGGCGTCCCCTTCGCCCACCATAATTGACGAACGCTCACTGGT‑GC‑CCCCCC‑GGGCTTGCCAGGAAACTTAACACCGGTTTGGTc                                                  <  1:5450773/93‑1 (MQ=255)

                                        ttGCGGTCGTGGCGTCCACTTCGCCCACCATAATTGACGAACGCTCACTGGT‑GC‑CCCCCC‑GGGCTTGCCAGGAAACTTAACACCGGTTTGGTc                                                  >  1:6624980/1‑93 (MQ=255)

                                         tGCGGTCGTGGCGTCCCCTCCGCCCACCATAATTGACGAACGCTCACTGGT‑GC‑CCCCCC‑GGGCTTGCCAGGAAACTTAACACCGGTTTGGTcc                                                 <  2:6404100/93‑1 (MQ=255)

                                          gCGGTCGTGGCGTCCACTTCGCCCACCATAATTGACGAACGCTCACTGGT‑GC‑CCCCCC‑GGGCTTGCCAGGAAACTTAACACCGGTTTGGTCCg                                                >  2:7688246/1‑93 (MQ=255)

                                          gCGGTCGTGGCGTCCACTCCGCCCACCATAATTGACGAACGCTCACTGGT‑GC‑CCCCCC‑GGGCTTGCCAGGAAACTTAACACCGGTTTGGTCCg                                                <  2:4892035/93‑1 (MQ=255)

                                             gTCGTGGCGTCCACTTCGCCCACCATAATTGACGAACGCTCACTGGT‑GC‑CCCCCC‑GGGCTTGCCAGGAAACTTCACACCGGTTTGGTCCGttt                                             >  2:1844905/1‑93 (MQ=255)

                                             gTCGTGGCGTCCACTTCGCCCACCATAATTGACGAACGCTCACTGGT‑GC‑CCCCCC‑GGGCTTGCCAGGAAACTTAACACCGGTTTGGTCCGttt                                             >  2:5892236/1‑93 (MQ=255)

                                             gTCGTGGCGTCCACTTCGCCCACCATAATTGACGAACGCTCACTGGT‑GC‑CCCCCC‑GGGCTTGCCAGGAAACTTAACACCGGTTTGGTCCGttt                                             >  1:3164347/1‑93 (MQ=255)

                                               cGTGCCCTCCCCTTCGCCCACCATAATTGACGAACGCTCACTGGT‑GC‑CCCCCC‑GGGCTTGCCAGGAAACTTAACACCGGTTTGGTCCGTTTGa                                           <  2:1094803/93‑1 (MQ=255)

                                                gTGGCGTCCCCTTCGCCCACCATAATTGACGAACGCTCACTGGT‑GC‑CCCCCC‑GGGCTTGCCAGGAAACTTAACACCGGTTTGGTCCGTTTGAc                                          <  1:4466071/93‑1 (MQ=255)

                                                gTGGCGTCCACCTCGCCCACCATAATTGACGAACGCTCACTGGT‑GC‑CCCCCC‑GGGCTTGCCAGGAAACTTAACACCGGTTTGGTCCGTTTGAc                                          <  1:2811290/93‑1 (MQ=255)

                                                 tgccGTCCCCTTCGCCCACCATAATTGACGAACGCTCACTGGT‑GC‑CCCCCC‑GGGCTTGCCAGGAAACTTAACACCGGTTTGGTCCGTTTGACt                                         <  2:2203831/90‑1 (MQ=255)

                                                 tGGCGTCCACTTCGCCCACCATAATTGACGAACGCTCACTGGT‑GCCCCCCCC‑GGGCTTGCCAGGAAACTTAACACCGGTTTGGTCCGTTTGAc                                          >  1:3026827/1‑93 (MQ=255)

                                                  ggCGTCCCCTCCGCCCACCATAATTGACGAACGCTCACTGGT‑GCCCCCCCC‑GGGCTTGCCAGGAAACTTAACACCGGTTTGGTCCGTTTGACt                                         <  1:867454/93‑1 (MQ=255)

                                                   gCGTCCACTTCGCCCACCATAATTGACGAACGCTCACTGGT‑GC‑CCCCCC‑GGGCTTGCCAGGAAACTTAACACCGGTTTGGTCCGTTTGACTAt                                       >  1:6283633/1‑93 (MQ=255)

                                                     gTCCACTTCGCCCACCATAATTGACGAACGCTCACTGGT‑GC‑CCCCCC‑GGGCTTGCCAGGAAACTTAACACCGGTTTGGTCCGTTTGACTATCa                                     >  2:6541601/1‑93 (MQ=255)

                                                     ctCCACTTCGCCCACCATAATTGACGAACGCTCACTGGT‑GC‑CCCCCC‑GGGCTTGCCAGGAAACTTAACACCGGTTTGGTCCGTTTGACTATCa                                     <  2:3607964/92‑1 (MQ=255)

                                                      tCCACTTCGCCCACCATAATTGACGAACGCTCACTGGT‑GC‑CCCCCC‑GGGCTTGCCAGGAAACTTAACACCGGTTTGGTCCGTTTGACTATCAg                                    >  2:7851279/1‑93 (MQ=255)

                                                       ccACTTCGCCCACCATAATTGACGAACGCTCACTGGT‑GC‑CCCCCC‑GGGCTTGCCAGGAAACTTAACACCGGTTTGGTCCGTTTGACTATCAGt                                   >  2:7787896/1‑93 (MQ=255)

                                                       ccACTTCGCCCACCATAATTGACGAACGCTCACTGGT‑GC‑CCCCCC‑GGGCTTGCCAGGAAACTTAACACCGGTTTGGTCCGTTTGACTATCAGt                                   >  2:3710697/1‑93 (MQ=255)

                                                       ccACTTCGCCCACCATAATTGACGAACGCTCACTGGT‑GC‑CCCCCC‑GGGCTTGCCAGGAAACTTAACACCGGTTTGGTCCGTTTGACTATCAGt                                   >  2:5417904/1‑93 (MQ=255)

                                                         ccTTCGCCCACCATAATTGACGAACGCTCACTGGT‑GCCCCCCCC‑GGGCTTGCCAGGAAACTTAACACCGGTTTGGTCCGTTTGACTATCAGTc                                  <  1:2005343/92‑1 (MQ=255)

                                                         ccTTCGCCCACCATAATTGACGAACGCTCACTGGT‑GC‑CCCCCC‑GGGCTTGCCAGGAAACTTAACACCGGTTTGGTCCGTTTGACTATCAGTcc                                 <  2:1285745/92‑1 (MQ=255)

                                                         ccTTCGCCCACCATAATTGACGAACGCTCACTGGT‑GC‑CCCCCC‑GGGCTTGCCAGGAAACTTAACACCGGTTTGGTCCGTTTGACTATCAGTcc                                 <  2:6213136/92‑1 (MQ=255)

                                                         aCTTCGCCCACCATAATTGACGAACGCTCACTGGT‑GCCCCCCCC‑GGGCTTGCCAGGAAACTTAACACCGGTTTGGTCCGTTTGACTATCAGTc                                  >  1:4135538/1‑93 (MQ=255)

                                                         aCTTCGCCCACCATAATTGACGAACGCTCACTGGT‑GC‑CCCCCC‑GGGCTTGCCAGGAAACTTAACACCGGTTTGGTCCGTTTGACTATCAGTcc                                 >  1:3116471/1‑93 (MQ=255)

                                                           ttCGCCCACCATAATTGACGAACGCTCACTGGT‑GC‑CCCCCC‑GGGCTTGCCAGGAAACTTAACACCGGTTTGGTCCGTTTGACTATCAGTCCCg                               >  2:343410/1‑93 (MQ=255)

                                                            tcccccACCATAATTGACGAACGCTCACTGGTCC‑‑CCCCCC‑GGGCTTGCCAGGAAACTTAACACCGGTTTGGTCCGTTTGACTATCAGTCCCGc                              <  1:5143836/90‑1 (MQ=255)

                                                             cccccACCATAATTGACGAACGCTCACTGGT‑GC‑CCCCCC‑GGGCTTGCCAGGAAACTTAACACCGGTTTGGTCCGTTTGACTATCAGTCCCGcc                             <  2:5545925/91‑1 (MQ=255)

                                                                  accaTAATTGACGAACGCTCACTGGG‑GC‑CCCCCC‑GGGCTTGCCAGGAAACTTAACACCGGTTTGGTCCGTTTGACTATCAGTCCCGCCTCTTc                        >  1:5635739/1‑93 (MQ=255)

                                                                   ccaTAATTGACGAACGCTCACTGGT‑GC‑CCCCCC‑GGGCTTGCCAGGAAACTTAACACCGGTTTGGTCCGTTTGACTATCAGTCCCGCCTCTTCa                       >  1:7220375/1‑93 (MQ=255)

                                                                    caTAATTGACGAACGCTCACTGGT‑GC‑CCCCCC‑GGGCTTGCCAGGAAACTTAACACCGGTTTGGTCCGTTTGACTATCAGTCCCGCCTCTTCaa                      >  1:2530375/1‑93 (MQ=255)

                                                                    caTAATTGACGAACGCTCACTGGT‑GC‑CCCCCC‑GGGCTTGCCAGGAAACTTAACACCGGTTTGGTCCGTTTGACTATCAGTCCCGCCTCTTCaa                      >  2:4144291/1‑93 (MQ=255)

                                                                       aaTTGACGAACGCTCACTGGT‑GC‑CCCCCC‑GGGCTTGCCAGGAAACTTAACACCGGTTTGGTCCGTTTGACTATCAGTCCCGCCTCTTCAATCg                   >  1:7068253/1‑93 (MQ=255)

                                                                          tggcGAACGCTCACTGGTCC‑‑CCCCCC‑GGGCTTGCCAGGAAACTTAACACCGGTTTGGTCCGTTTGACTATCAGTCCCGCCTCTTCAATCGCtt                <  2:468870/90‑1 (MQ=255)

                                                                          tGACGACCGCTCACTGGTCC‑‑CCCCCC‑GGGCTTCCCAGGAAACTTAACACCGGTTTGGTCCGTTTGACTATCAGTCCCGCCTCTTCAATCGCtt                <  2:859548/93‑1 (MQ=255)

                                                                           gACGAACGCTCACTGGG‑GC‑CCCCCC‑GGGCTTGCCAGGAAACTTAACACCGGTTTGGTCCGTTTGACTATCAGTCCCGCCTCTTCAATCGCTTc               <  1:580260/93‑1 (MQ=255)

                                                                           gACGAACGCTCACGGGT‑GC‑CCCCCC‑GGGCTTGCCAGGAAACTTAACACCGGTTTGGTCCGTTTGACTATCAGTCCCGCCTCTTCAATCGCTTc               <  1:4086210/93‑1 (MQ=255)

                                                                            aCGAACGCTCACTGGT‑GCCCCCCCC‑GGGCTTGCCAGGAAACTTAACACCGGTTTGGTCCGTTTGACTATCAGTCCCGCCTCTTCAATCGCTTc               <  1:3999551/93‑1 (MQ=255)

                                                                             cGAACGCTCACTGGT‑GC‑CCCCCC‑GGGCTTGCCAGGAAACTTAACACCGGTTTGGTCCGTTTGACTATCAGTCCCGCCTCTTCAATCGCTTcgc             <  2:4881869/93‑1 (MQ=255)

                                                                             cGAACGCTCACTGGT‑GC‑CCCCCC‑GGGCTTGCCAGGAAACTTAACACCGGTTTGGTCCGTTTGACTATCAGTCCCGCCTCTTCAATCGCTTcgc             <  2:2527605/93‑1 (MQ=255)

                                                                              gAACGCTCACTGGT‑G‑‑CCCCCC‑GGGCTTGCCAGGAAACTTAACACCGGTTTGGTCCGTTTGACTATCAGTCCCGCCTCTTCAATCGCTTCGCGa           >  2:1982936/1‑93 (MQ=255)

                                                                               accgctcgctGGT‑G‑‑CCCCCCGGGGCTTGCGAGGAAACTTAGCGCGGGTTTGGTCCGTTTGACTATCAGTCCCGCCTCTTCAATCGCTTCGCGa           <  1:3316826/91‑1 (MQ=255)

                                                                               aaCGCTCACTGGT‑GC‑CCCCCC‑GGGCTTGCCAGGAAACTTAACACCGGTTTGGTCCGTTTGACTATCAGTCCCGCCTCTTCAATCGCTTCGCGa           <  2:3909287/93‑1 (MQ=255)

                                                                               aaCGCTCACTGGT‑GC‑CCCCCC‑GGGCTTGCCAGGAAACTTAACACCGGTTTGGTCCGTTTGACTATCAGTCCCGCCTCTTCAATCGCTTCGCGa           <  1:2900033/93‑1 (MQ=255)

                                                                                     cACTGGT‑GC‑CCCCCC‑GGGCTTGCCAGGAAACTTAACACCGGTTTGGTCCGTTTGACTATCAGTCCCGCCTCTTCAATCGCTTCGCGACGGGCa     >  2:3316674/1‑93 (MQ=255)

                                                                                      ccTGGT‑GC‑CCCCCC‑GGGCTTGCCAGGAAACTTAACACCGGTTTGGTCCGTTTGACTATCAGTCCCGCCTCTTCAATCGCTTCGCGACGGGCaa    <  2:6679688/92‑1 (MQ=255)

                                                                                      aCTGGT‑GC‑CCCCCC‑GGGCTTGCCAGGAAACTTAACACCGGTTTGGTCCGTTTGACTATCAGTCCCGCCTCTTCAATCGCTTCGCGCCGGGCac    >  2:3138223/1‑92 (MQ=255)

                                                                                       cTGGT‑GC‑CCCCCC‑GGGCTTGCCAGGAAACTTAACACCGGTTTGGTCCGTTTGACTATCAGTCCCGCCTCTTCAATCGCTTCGCGACGGGCAAc   >  2:2971958/1‑93 (MQ=255)

                                                                                       cTGGT‑GC‑CCCCCC‑GGGCTTGCCAGGAAACTTAACACCGGTTTGGTCCGTTTGACTATCAGTCCCGCCTCTTCAATCGCTTCGCGACGGGCAAc   >  1:5554/1‑93 (MQ=255)

                                                                                        tGGT‑GC‑CCCCCC‑GGGCTTGCCAGGAAACTTAACACCGGTTTGGTCCGTTTGACTATCAGTCCCGCCTCTTCAATCGCTTCGCGACGGGCAACa  >  2:3079502/1‑93 (MQ=255)

CGAATATCTTCGTTTTCATCAGCCAGACCGTGAATACCGCTTGCGGTCGTGGCGTCCACTTCGCCCACCATAATTGACGAACGCTCACTGGT‑G‑‑CCCCCC‑GGGCTTGCCAGGAAACTTAACACCGGTTTGGTCCGTTTGACTATCAGTCCCGCCTCTTCAATCGCTTCGCGACGGGCAACA  >  NC_000913/3175427‑3175606

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 18 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

ATCTTCGTTTTCATCAGCCAGACCGTGAATACCGCTTGCGGTCGTGGCGTCCACTTCGCCCACCATAATTGACGAACGCTCACTGGT‑G‑‑CCCCCC‑GGGCTTGCCAGGAAACTTAACACCGGTTTGGTCCGTTTGACTATCAGTCCCGCCTCTTCAATCGCTTCGCGACGGGCAACATCTTCCACACTT  >  NC_000913/3175432‑3175618

ATCTTCGTTTTCATCAGCCAGACCGTGAATACCGCTTGCGGTCGTGGCGTCCACTTCGCCCACCATAATTGACGAACGCTCACTGGT‑G‑‑CCCCCC                                                                                                >  SRR4302543.4617714/1‑94 (MQ=60)

ATCTTCGTTTTCATCAGCCAGACCGTGAATACCGCTTGCGGTCGTGGCGTCCACTTCGCCCACCATAATTGACGAACGCTCACTGGT‑G‑‑CCCCCC                                                                                                >  SRR4302543.2140956/1‑94 (MQ=60)

ATCTTCGTTTTCATCAGCCAGACCGTGAATACCGCTTGCGGTCGTGGCGTCCACTTCGCCCACCATAATTGACGAACGCTCACTGGT‑G‑‑CCCCCC                                                                                                >  SRR4302543.770947/1‑94 (MQ=60)

ATCTTCGTTTTCATCAGCCAGACCGTGAATACCGCTTGCGGTCGTGGCGTCCACTTCGCCCACCATAATTGACGAACGCTCACTGGT‑G‑‑CCCCCC                                                                                                >  SRR4302543.7046713/1‑94 (MQ=60)

ATCTTCGTTTTCATCAGCCAGACCGTGAATACCGCTTGCGGTCGTGGCGTCCACTTCGCCCACCATAATTGACGAACGCTCACTGGT‑G‑‑CCCCCC                                                                                                >  SRR4302543.4496593/1‑94 (MQ=60)

ATCTTCGTTTTCATCAGCCAGACCGTGAATACCGCTTGCGGTCGTGGCGTCCACTTCGCCCACCATAATTGACGAACGCTCACTGGG‑G‑‑CCCCCC                                                                                                >  SRR4302543.6782034/1‑94 (MQ=60)

 TCTTCGTTTTCATCAGCCAGACCGTGAATACCGCTTGCGGTCGTGGCGTCCACTTCGCCCACCATAATTGACGAACGCTCACTGGT‑G‑‑CCCCCC‑C                                                                                              <  SRR4302543.4262548/94‑1 (MQ=60)

 TCTTCGTTTTCATCAGCCAGACCGTGAATACCGCTTGCGGTCGTGGCGTCCACTTCGCCCACCATAATTGACGAACGCTCACTGGT‑G‑‑CCCCCC‑C                                                                                              >  SRR4302543.6149406/1‑94 (MQ=60)

  CTTCGTTTTCATCAGCCAGACCGTGAATCCCGCTTGCGGTCGTGGCGTCCACTTCGCCCACCATAATTGACGAACGCTCACTGGT‑G‑‑CCCCCC‑CG                                                                                             <  SRR4302543.2368463/94‑1 (MQ=60)

  CTTCGTTTTCATCAGCCAGACCGTGAATACCGCTTGCGGTCGTGTCGTCCACTTCGCCCACCATAATTAACGAACGCTCACTGGA‑G‑‑CCCCCC‑AG                                                                                             >  SRR4302543.2280591/1‑94 (MQ=60)

  CTTCGTTTTCATCAGCCAGACCGTGAATACCGCTTGCGGTCGTGGCGTCCACTTCGCCCACCATAATTGACGAACGCTCACTGGT‑G‑‑CCCCCC‑CG                                                                                             >  SRR4302543.7110059/1‑94 (MQ=60)

  CTTCGTTTTCATCAGCCAGACCGTGAATACCGCTTGCGGTCGTGGCGTCCACTTCGCCCACCATAATTGACGAACGCTCACTGGT‑G‑‑CCCCCC‑CG                                                                                             <  SRR4302543.4521253/94‑1 (MQ=60)

   TTCTTTTTCATCAGCCAGACCGTGAATCCCGCTGGCGGTCGTGGCGTCCACTTCGCCCACCATAATTGACGAACGCTCACTGGT‑G‑‑CCCCCC‑CGG                                                                                            <  SRR4302543.1570271/94‑1 (MQ=60)

    TCTTTTGCATCAGCCAGCCCGTGAATCCCGCTTGCGGTCGTGGCGTCCACTTCGCCCACCATAATTGACGAACGCTCACTGGT‑GC‑CCCCCC‑GGG                                                                                            <  SRR4302543.3567004/94‑1 (MQ=60)

    TCGTTTTCATCAGCCAGACCGTGAATCCCGCTTGCGGTCGTGGCGTCCACTTCGCCCACCATAATTGACGAACGCTCACTGGT‑GC‑CCCCCC‑GGG                                                                                            <  SRR4302543.735541/94‑1 (MQ=60)

      gctatcatcagccaaacggtgaaaacagttacggatcgtggccccccgcTCGCCCACCATAATTGACGAACGCTCACTGGT‑GC‑CCCCCC‑GGGCT                                                                                          <  SRR4302543.3922643/45‑1 (MQ=60)

      GTTTTCATCAGCCAGACCGTGAATACCGCTTGCGGTCGTGGCGTCCACTTCGCCCACCATAATTGACGAACGCTCACTGGT‑GC‑CCCCCC‑GGGCT                                                                                          >  SRR4302543.4518422/1‑94 (MQ=60)

      GTTTTCATCAGCCAGACCGTGAATACCGCTTGCGGTCGTGGCGTCCACTTCGCCCACCATAATTGACGAACGCTCACTGGT‑GC‑CCCCCC‑GGGCT                                                                                          >  SRR4302543.4557824/1‑94 (MQ=60)

       TTTTCATCAGCCAGACCGTGAATACCGCTTGCGGTCGTGGCGTCCACTTCGCCCACCATAATTGACGAACGCTCACTGGT‑GC‑CCCCCC‑GGGCTT                                                                                         >  SRR4302543.3678519/1‑94 (MQ=60)

       TTTTCATCAGCCAGACCGTGAATACCGCTTGCGGTCGTGGCGTCCACTTCGCCCACCATAATTGACGAACGCTCACTGGT‑GC‑CCCCCC‑GGGCTT                                                                                         >  SRR4302543.4053256/1‑94 (MQ=60)

         TTCATCAGCCAGACCGTGAATACCGCTTGCGGTCGTGGCGTCCACTTCGCCCACCATAATTGACGAACGCTCACTGGT‑GC‑CCCCCC‑GGGCTTGC                                                                                       >  SRR4302543.7205557/1‑94 (MQ=60)

          TCATCAGCCAGACCGTGAATACCGCTTGCGGTCGTGGCGTCCACTTCGCCCACCATAATTGACGAACGCTCACTGGT‑GC‑CCCCCC‑GGGCTTGCC                                                                                      >  SRR4302543.6663891/1‑94 (MQ=60)

            ATCAGCCAGACCGTGAATACCGCTTGCGGTCGTGGCGTCCACTTCGCCCACCATAATTGACGAACGCTCACTGGT‑GC‑CCCCCC‑GGGCTTGCCAG                                                                                    >  SRR4302543.4592492/1‑94 (MQ=60)

            ATCAGCCAGACCGTGAATACCGCTTGCGGTCGTGGCGTCCACTTCGCCCACCATAATTGACGAACGCTCACTGGT‑GC‑CCCCCC‑GGGCTTGCCAG                                                                                    >  SRR4302543.596130/1‑94 (MQ=60)

            ATCAGCCAGACCGTGAATACCGCTTGCGGTCGTGGCGTCCACTTCGCCCACCATAATTGACGAACGCTCACTGGT‑GC‑CCCCCC‑GGGCTTGCCAG                                                                                    >  SRR4302543.6000274/1‑94 (MQ=60)

             TCAGCCAGCCCGTGAATACCGCTTGCGGTCGTGGCGTCCACTTCGCCCACCATAATTGACGAACGCTCACTGGT‑GC‑CCCCCC‑GGGCTTGCCAGG                                                                                   <  SRR4302543.5521347/94‑1 (MQ=60)

             TCAGCCAGACGGTGAATCCCGTTTTGGGTCGTGGCGTCTCTTTCGCCCACCATAATTGACGAACGCTCACTGGT‑GC‑CCCCCC‑GGGCTTGCCAGG                                                                                   <  SRR4302543.5521346/94‑1 (MQ=46)

              CAGCCAGACCGTGAATACCGCTTGCGGTCGTGGCGTCCACTTCGCCCACCATAATTGACGAACGCTCACTGGT‑G‑‑CCCCCCccgggttgcccgga                                                                                  >  SRR4302543.6020658/1‑80 (MQ=60)

               CGCCAGACCGTGAATACCGCTTGCGGTCGTGGCGTCCACTCCGCCCACCATAATTGACGAACGCTCACTGGT‑GC‑CCCCCC‑GGGCTTGCCAGGAA                                                                                 <  SRR4302543.2085030/94‑1 (MQ=60)

               AGCCAGACCGTGAATACCGCTTGCGGTCGTGGCGTCCACTTCGCCCACCATAATTGACGAACGCTCACTGGT‑GC‑CCCCCC‑GGGCTTGCCAGGAA                                                                                 >  SRR4302543.4411960/1‑94 (MQ=60)

               AGCCAGACCGTGAATACCGCTTGCGGTCGTGGCGTCCACTTCGCCCACCATAATTGACGAACGCTCACTGGT‑GC‑CCCCCC‑GGGCTTGCCAGGAA                                                                                 >  SRR4302543.491716/1‑94 (MQ=60)

                GCCAGACCGTGAATACCGCTTGCGGTCGTGGCGCCCCCTTCGCCCACCATAATTGACGAACGCTCACTGGT‑GCCCCCCCC‑GGGCTTGCCAGGAA                                                                                 <  SRR4302543.789520/94‑1 (MQ=60)

                 CCAGACCGTGAATACCGCTTGCGGTCGTGGCGTCCACTTCGCCCACCATAATTGACGAACGCTCACTGGT‑GCCCCCCCC‑GGGCTTGCCAGGAAA                                                                                >  SRR4302543.5057324/1‑94 (MQ=60)

                 CCAGACCGTGAATACCGCTTGCGGTCGTGGCGTCCACTTCGCCCACCATAATTGACGAACGCTCACTGGT‑GC‑CCCCCC‑GGGCTTGCCAGGAAAC                                                                               <  SRR4302543.4333091/94‑1 (MQ=60)

                  CAGACCGTGAATCCCCCTTGCGGTCGTGGCGTCCCCTTCGCCCACCATAATTGACGAACGCTCACTGGT‑GC‑CCCCCC‑GGGCTTGCCAGGAAACT                                                                              <  SRR4302543.1739577/94‑1 (MQ=60)

                  CAGACCGTGAATACCGCTTGCGGTCGTGGCGTCCACTTCGCCCACCATAATTGACGAACGCTCACTGGT‑GC‑CCCCCC‑GGGCTTGCCAGGAAACT                                                                              >  SRR4302543.6525103/1‑94 (MQ=60)

                  CAGACCGTGAATACCGCTTGCGGTCGTGGCGTCCACTTCGCCCACCATAATTGACGAACGCTCACTGGT‑GC‑CCCCCC‑GCGCTTGCCAGGAAACT                                                                              >  SRR4302543.7163447/1‑94 (MQ=60)

                  CAGACCGTGAATACCGCTTGCGGCCGTGGCGTCCACTTCGCTCACTCTAATTAACGAACGCTCACTGGG‑GT‑CCCCCC‑GGGCTTGCCAGGACACT                                                                              >  SRR4302543.2426016/1‑94 (MQ=60)

                   AGACCGTGAATACCGCTTGCGGTCGTGGCGTCCACTTCGCCCACCATAATTGACGAACGCTCACTGGT‑GCCCCCCCC‑GGGCTTGCCAGGAAACT                                                                              >  SRR4302543.5209951/1‑94 (MQ=60)

                     ACCGTGAATACCGCTTGCGGTCGTGGCGTCCACTTCGCCCACCATAATTGACGAACGCTCACTGGT‑GC‑CCCCCC‑GGGCTTGCCAGGAAACTTAA                                                                           >  SRR4302543.4702299/1‑94 (MQ=60)

                     ACCGTGAATACCGCTTGCGGTCGTGGCGTCCACTTCGCCCACCATAATTGACGAACGCTCACTGGT‑GC‑CCCCCC‑GGGCTTGCCAGGAAACTTAA                                                                           >  SRR4302543.4702297/1‑94 (MQ=60)

                     ACCGTGAATACCGCTTGCGGTCGTGGCGTCCACTTCGCCCACCATAATTGACGAACGCTCACTGGT‑GC‑CCCCCC‑GGGCTTGCCAGGAAACTTAA                                                                           >  SRR4302543.5334884/1‑94 (MQ=60)

                     ACCGTGAATACCGCTTGCGGTCGTGGCGTCCACTTCGCCCACCATAATTGACGAACGCTCACTGGT‑GC‑CCCCCC‑GGGCTTGCCAGGAAACTTAA                                                                           >  SRR4302543.2942011/1‑94 (MQ=60)

                      CCGTGAATCCCGCTTGCGTTCGTGCCGTCCACTTCCCCCACCATAATTGACGAACGCTCACTGGT‑GC‑CCCCCC‑GGGCTTGCCAGGAAACTTAAC                                                                          <  SRR4302543.6017913/94‑1 (MQ=60)

                      CCGTGAATCCCGCTTGCGGTCGTGGCGTCCACTTCGCCCACCATAATTGACGAACGCTCACTGGT‑GC‑CCCCCC‑GGGCTTGCCAGGAAACTTAAC                                                                          <  SRR4302543.5474744/94‑1 (MQ=60)

                       CGTGAATACCGCTTGCGGTCGTGGCGTCCACTTCGCCCAACATAATTGACGAACGCTAACTGGT‑GC‑CCCCCC‑GGGCTTGCCAGGAAACTacaca                                                                         >  SRR4302543.5931891/1‑89 (MQ=60)

                         nnnatccncCTTGCGGTNGTGNCNTCCCCTTCCCCCACCATAATTGACGAACGCTCACTGGT‑GC‑CCCCCC‑GGGCTTGCCAGGAAACTTAACACC                                                                       <  SRR4302543.5644209/85‑1 (MQ=60)

                         TGAATCCCGCTTGCGGTCGTGCCGTCCACTTCGCCCACCATAATTGACGAACGCTCACTGGT‑GCCCCCCCC‑GGGCTTGCCAGGAAACTTAACAC                                                                        <  SRR4302543.1193061/94‑1 (MQ=60)

                         TGAATACCGCTTGCGGTCGTGGCGTCCACTTCGCCCACCATAATTGACGAACGCTCACTGGT‑GC‑CCCCCC‑GGGCTGGCCAGTAAACTTAACGCC                                                                       >  SRR4302543.1132675/1‑94 (MQ=60)

                          aaacaccgccgGCGGTCGTGGCGTCCACTCCGCCCACCATAATTGACGAACTCTCACTGGT‑GC‑CCCCCC‑GGGCTTGCCAGGAAACTTAACACCG                                                                      <  SRR4302543.4933291/83‑1 (MQ=60)

                          GAATACCGCTTGCGGTCGTGGCGTCCACTTCGCCCACCATAATTGACGAACGCTCACTGGT‑GCCCCCCCC‑GGGCTTGCCAGGAAACTTAACACC                                                                       >  SRR4302543.7134470/1‑94 (MQ=60)

                          GAATACCGCTTGCGGTCGTGGCGTCCACTTCGCCCACCATAATTGACGAACGCTCACTGGT‑GC‑CCCCCC‑GGGCTTGCCAGGAAACTTAACACCG                                                                      >  SRR4302543.2810484/1‑94 (MQ=60)

                          GAATACCGCTTGCGGTCGTGGCGTCCACTTCGCCCACCATAATTGACGAACGCTCACTGGT‑GC‑CCCCCC‑GGGCTTGCCAGGAAACTTAACACCG                                                                      >  SRR4302543.194618/1‑94 (MQ=60)

                          GAAGACCGCTTGCGGTCGTGGCGTCCACTCCGCCCACCATAATTGACGAACGCTCACTGGT‑GC‑CCCCCC‑GGGCTTGCCAGGAAACTTAACACCG                                                                      <  SRR4302543.3371376/94‑1 (MQ=60)

                           AATACCGCTTGCGGTCGTGGCGTCCACTTCGCCCACCATAATTGACGAACGCTCACTGGT‑GC‑CCCCCC‑GGGCTTGCCAGGAAACTTAACACCGG                                                                     >  SRR4302543.1706288/1‑94 (MQ=60)

                            ATACCGCTTGCGGTCGTGGCGTCCACTTCCCCCACCATAATTGACGAACGCTCACTGGT‑GC‑CCCCCC‑GGGCTTGCCAGGAAACTTAACACCGGT                                                                    <  SRR4302543.5072674/94‑1 (MQ=60)

                             TACCGCTTGCGGTCGTGGCGTCCACTTCGCCCACCATAATTGACGAACGCTCACTGGT‑GC‑CCCCCC‑GGGCTTGCCAGGAAACTTAACACCGGTT                                                                   >  SRR4302543.7808030/1‑94 (MQ=60)

                              ccccCTTGCGGTCGTGTCGCCCACTTCGCCCACCATAATTGACGAACGCTCACTGGT‑GC‑CCCCCC‑GGGCTTGCCAGGAAACTTAACACCGGTTT                                                                  <  SRR4302543.4845794/90‑1 (MQ=60)

                               CCGCTTGCGGTCGTGGCGTCCCCTTCGCCCACCATAATTGACGAACGCTCACTGGT‑GC‑CCCCCC‑GGGCTTGCCAGGAAACTTAACACCGGTTTG                                                                 <  SRR4302543.3580896/94‑1 (MQ=60)

                               CCGCTTGCGGTCGTGGCGTCCACTTCGCCCACCATAATTGACGAACGCTCACTGGT‑GCCCCCCCC‑GGGCTTGCCAGGAAACTTAACACCGGTTT                                                                  >  SRR4302543.5311908/1‑94 (MQ=60)

                               CCGCTTGCGGTCGTGGCGTCCACTTCGCCCACCATAATTGACGAACGCTCACTGGT‑GC‑CCCCCC‑GGGCTTGCCAGGAAACTTAACACCGGTTTG                                                                 <  SRR4302543.2444981/94‑1 (MQ=60)

                               CCGCTTGCGGTCGTGGCGTCCACTTCGCCCACCATAATTGACGAACGCTCACTGGT‑GC‑CCCCCC‑GGGCTTGCCAGGAAACTTAACACCGGTTTG                                                                 <  SRR4302543.2771709/94‑1 (MQ=60)

                               CCGCTTGCGGTCGTGGCGTCCACTCCGCCCACCATAATTGACGAACGCTCACTGGT‑GC‑CCCCCC‑GGGCTTGCCATGAAACTTAACACCGGTTTG                                                                 <  SRR4302543.4651272/94‑1 (MQ=60)

                                cgtttccgtTCGTGGCGTCCACTGCGCCCACCATAATTGACGAACGCTCACTGGT‑GC‑CCCCCC‑GGGCTTGCCAGGAAACTTAACACCGGTTTGG                                                                <  SRR4302543.998670/85‑1 (MQ=60)

                                cgcttgcggtgggggctccccctgCGCCCACCATAATTGACGAACGCTCACTGGT‑GC‑CCCCCC‑GGGCTTGCCAGGAAACTTAACACCGGTTTGG                                                                <  SRR4302543.3390748/70‑1 (MQ=60)

                                CGCTTGCGGTCGTGGCGTCCACTTCGCCCACCATAATTGACGAACGCTCACTGGT‑GC‑CCCCCC‑GGGCTTGCCAGGAAACTTAACACCGGTTTGG                                                                >  SRR4302543.4140611/1‑94 (MQ=60)

                                CGCTTGCGGTCGTGGCGTCCACTTCGCCCACCATAATTGACGAACGCTCACTGGT‑GC‑CCCCCC‑GGGCTTGCCAGGAAACTTAACACCGGTTTGG                                                                >  SRR4302543.1517555/1‑94 (MQ=60)

                                 GCTTGCGGTCGTGGCGTCCACTTCGCCCACCATAATTGACGAACGCTCACTGGT‑GC‑CCCCCC‑GGGCTTGCTAGGAAACTTAACACCGGTTTGGT                                                               >  SRR4302543.994542/1‑94 (MQ=60)

                                 GCTTGCGGTCGTGGCGTCCACTTCGCCCACCATAATTGACGAACGCTCACTGGT‑GC‑CCCCCC‑GGGCTTGCTAGGAAACTTAACACCGGTTTGGT                                                               >  SRR4302543.994541/1‑94 (MQ=60)

                                 GCTTGCGGTCGTGGCGTCCACTTCGCCCACCATAATTGACGAACGCTCACTGGT‑GC‑CCCCCC‑GGGCTTGCCAGGAAACTTAACACCGGTTTGGT                                                               >  SRR4302543.2627626/1‑94 (MQ=60)

                                 GCTTGCGGTCGTGGCGTCCACTTCGCCCACCATAATTGACGAACGCTCACTGGT‑GC‑CCCCCC‑GGGCTTGCCAGGAAACTTAACACCGGTTTGGT                                                               >  SRR4302543.7178805/1‑94 (MQ=60)

                                 GCTTGCGGTCGTGGCGTCCACTCCGCCCACCATAATTGACGAACGCTCACTGGT‑GC‑CCCCCC‑GGGCTTGCCAGGAAACTTAACACCGGTTTGGT                                                               <  SRR4302543.7139027/94‑1 (MQ=60)

                                 CCTTGCGGTGGTGGCGTCCACTTCGCCCACCATAATTGACGAACGCTCACTGGT‑GC‑CCCCCC‑GGGCTTGCCAGGAAACTTAACACCGGTTTGGT                                                               <  SRR4302543.1784515/94‑1 (MQ=60)

                                  CTTGCGGTCGTGGCGTCCACTTCGCCCACCATAATTGACGAACGCTCACTGGT‑GC‑CCCCCC‑GGGCTTGCCAGGAAACTTAACACCGGTTTGGTC                                                              >  SRR4302543.6687459/1‑94 (MQ=60)

                                  CTTGCGGTCGTGGCGTCCACTTCGCCCACCATAATTAACGAACGCTCACTGGG‑GC‑CCCCCC‑ATGCTTCCCAGGAAACTTAACAACGGTTTGGTC                                                              >  SRR4302543.2778313/1‑94 (MQ=60)

                                   TTGCGGTCGTGGCGTCCCCTTCGCCCACCATAATTGACGAACGCTCACTGGT‑GC‑CCCCCC‑GGGCTTGCCAGGAAACTTAACACCGGTTTGGTCC                                                             <  SRR4302543.5500212/94‑1 (MQ=60)

                                    TGCGGTCGTGGCGTCCACTTCGCCCACCATAATTGACGAACGCTCACTGGT‑GC‑CCCCCC‑GGGCTTGCCAGGAAACTTAACACCGGTTTGGTCCG                                                            >  SRR4302543.3175204/1‑94 (MQ=60)

                                      CGGTCGTGGCCTCCCCTTCGCCCACCATAATTGACGAACGCTCACTGGT‑GC‑CCCCCC‑GGGCTTGCCAGGAAACTTAACACCGGTTTGGTCCGTT                                                          <  SRR4302543.1149711/94‑1 (MQ=60)

                                       GGTCGTGGCGTCCACTTCGCCCACCATAATTGACGAACGCTCACTGGT‑GC‑CCCCCC‑GGGCTTGCCAGGAAACTTAACACCGGTTTGGTCCGTTT                                                         >  SRR4302543.3192835/1‑94 (MQ=60)

                                       GGTCGTGGCGTCCACTTCGCCCACCATAATTGACGAACGCTCACTGGT‑GA‑CCCCCC‑GGGCTTGCCAGGAAACTTCACACCGGTTTGGGCCGTTT                                                         >  SRR4302543.3192834/1‑94 (MQ=60)

                                        GTCGTGGCGTCCCCTCCGCCCACCATAATTGACGAACGCTCACTGGT‑GC‑CCCCCC‑GGGCTTGCCAGGAAACTTAACACCGGTTTGGTCCGTTTG                                                        <  SRR4302543.617943/94‑1 (MQ=60)

                                        GTCGTGGCGTCCACTTCGCCCACCATAATTGACGAACGCTCACTGGT‑GC‑CCCCCC‑GGGCTTGCCAGGAAACTTAACACCGGTTTGGTCCGTTTG                                                        >  SRR4302543.6773297/1‑94 (MQ=60)

                                         TCGGGGCGCCCACTTCGCCCACCATAATTGACGAACGCTCACTGGT‑GC‑CCCCCC‑GGGCTTGCCAGGAAACTTAACACCGGTTTGGTCCGTTTGA                                                       <  SRR4302543.2121036/94‑1 (MQ=60)

                                         GCGTGGCGTCCACTCCGCCCACCATAATTGACGAACGCTCACTGGT‑GC‑CCCCCC‑GGGCTTGCCAGGAAACTTAACACCGGTTTGGTCCGTTTGA                                                       <  SRR4302543.4360943/94‑1 (MQ=60)

                                           GTGGCGTCCCCTTCGCCCACCATAATTGACGAACGCTCACTGGT‑GC‑CCCCCC‑GGGCTTGCCAGGAAACTTAACACCGGTTTGGTCCGTTTGACT                                                     <  SRR4302543.4506553/94‑1 (MQ=60)

                                           GTGGCGTCCACTTCGCCCACCATAATTGACGAACGCTCACTGGT‑GCCCCCCCC‑GGGCTTGCCAGGAAACTTAACACCGGTTTGGTCCGTTTGAC                                                      >  SRR4302543.3054174/1‑94 (MQ=60)

                                           GTGGCGTCCACTTCCCCCACCATAATTGACGAACGCTCACTGGT‑GC‑CCCCCC‑GGGCTTGCCAGGAAACTTAACACCGGTTTGGTCCGTTTGACT                                                     <  SRR4302543.580673/94‑1 (MQ=60)

                                           GTGGCGTCCACCTCGCCCACCATAATTGACGAACGCTCACTGGT‑GC‑CCCCCC‑GGGCTTGCCAGGAAACTTAACACCGGTTTGGTCCGTTTGACT                                                     <  SRR4302543.2837028/94‑1 (MQ=60)

                                             GGCGTCCCCTCCGCCCACCATAATTGACGAACGCTCACTGGT‑GCCCCCCCC‑GGGCTTGCCAGGAAACTTAACACCGGTTTGGTCCGTTTGACTA                                                    <  SRR4302543.875206/94‑1 (MQ=60)

                                             GGCGTCCACTTCGCCCACCATAATTGACGAACGCTCACTGGT‑GC‑CCCCCC‑GGGCTTGCCAGGAAACTTAACACCGGTTTGGTCCGTTTGACTAT                                                   >  SRR4302543.6342833/1‑94 (MQ=60)

                                               CGTCCACTTCGCCCACCATAATTGACGAACGCTCACTGGT‑GC‑CCCCCC‑GGGCTTGCCAGGAAACTTAACACCGGTTTGGTCCGTTTGACTATCA                                                 <  SRR4302543.3552870/94‑1 (MQ=60)

                                               CGTCCACTTCGCCCACCATAATTGACGAACGCTCACTGGT‑GC‑CCCCCC‑GGGCTTGCCAGGAAACTTAACACCGGTTTGGTCCGTTTGACTATCA                                                 >  SRR4302543.2878829/1‑94 (MQ=60)

                                               CGTCCACTTCGCCCACCATAATTGACGAACGCTCACTGGT‑GC‑CCCCCC‑GGGCTTGCCAGGAAACTTAACACCGGTTTGGTCCGTTTGACTATCA                                                 >  SRR4302543.3673452/1‑94 (MQ=60)

                                               CGCCCCCTTCCCCCACCATAATTGACGAACGCTCACTGGT‑GC‑CCCCCC‑GGGCTTGCCAGGAAACTTAACACCGGTTTGGTCCGTTTGACTATCA                                                 <  SRR4302543.4007352/94‑1 (MQ=60)

                                                GTCCACTTCGCCCACCATAATTGACGAACGCTCACTGGT‑GC‑CCCCCC‑GGGCTTGCCAGGAAACTTAACACCGGTTTGGTCCGTTTGACTATCAG                                                >  SRR4302543.5876210/1‑94 (MQ=60)

                                                   CACTTCGCCCACCATAATTGACGAACGCTCACTGGT‑GCCCCCCCC‑GGGCTTGCCAGGAAACTTAACACCGGTTTGGTCCGTTTGACTATCAGTC                                              >  SRR4302543.4172861/1‑94 (MQ=60)

                                                   CACTTCGCCCACCATAATTGACGAACGCTCACTGGT‑GC‑CCCCCC‑GGGCTTGCCAGGAAACTTAACACCGGTTTGGTCCGTTTGACTATCAGTCC                                             >  SRR4302543.3144503/1‑94 (MQ=60)

                                                   CACTTCGCCCACCATAATTGACGAACGCTCACTGGT‑GC‑CCCCCC‑GGGCTTGCCAGGAAACTTAACACCGGTTTGGTCCGTTTGACTATCAGTCC                                             >  SRR4302543.4161480/1‑94 (MQ=60)

                                                    CCTTCGCCCACCATAATTGACGAACGCTCACTGGT‑GCCCCCCCC‑GGGCTTGCCAGGAAACTTAACACCGGTTTGGTCCGTTTGACTATCAGTCC                                             <  SRR4302543.2023363/94‑1 (MQ=60)

                                                    CCTTCGCCCACCATAATTGACGAACGCTCACTGGT‑GC‑CCCCCC‑GGGCTTGCCAGGAAACTTAACACCGGTTTGGTCCGTTTGACTATCAGTCCC                                            <  SRR4302543.179073/94‑1 (MQ=60)

                                                    ACTTCGCCCACCATAATTGACGAACGCTCACTGGT‑GC‑CCCCCC‑GGGCTTGCCAGGAAACTTAACACCGGTTTGGTCCGTTTGACTATCAGTCCC                                            >  SRR4302543.2661931/1‑94 (MQ=60)

                                                     CTCCGCCCACCATAATTGACGAACGCTCACTGGT‑GC‑CCCCCC‑GGGCTTGCCAGGAAACTTAACACCGGTTTGGTCCGTTTGACTATCAGTCCCG                                           <  SRR4302543.3486067/94‑1 (MQ=60)

                                                       TCCCCCACCATAATTGACGAACGCTCACTGGTCC‑‑CCCCCC‑GGGCTTGCCAGGAAACTTAACACCGGTTTGGTCCGTTTGACTATCAGTCCCGCC                                         <  SRR4302543.5190341/94‑1 (MQ=60)

                                                       CCGCCCACCATAATTGACGAACGCTCACTGGT‑GC‑CCCCCC‑GGGCTTGCCAGGAAACTTAACACCGGTTTGGTCCGTTTGACTATCAGTCCCGCC                                         <  SRR4302543.5297111/94‑1 (MQ=60)

                                                        CGCCCACCATAATTGACGAACGCTCACTGGT‑GC‑CCCCCC‑GGGCTTGCCAGGAAACTTAACACCGGTTTGGTCCGTTTGACTATCAGTCCCGCCT                                        <  SRR4302543.3073453/94‑1 (MQ=60)

                                                        CCCCCACCATAATTGACGAACGCTCACTGGT‑GC‑CCCCCC‑GGGCTTGCCAGGAAACTTAACACCGGTTTGGTCCGTTTGACTATCAGTCCCGCCT                                        <  SRR4302543.5655479/94‑1 (MQ=60)

                                                         GCCCACCATAATTGACGAACGCTCACTGGT‑GC‑CCCCCC‑GGGCTTGCCAGGAAACTTAACACCGGTTTGGTCCGTTTGACTATCAGTCCCGCCTC                                       >  SRR4302543.4281665/1‑94 (MQ=60)

                                                          CCCACCATAATTGACGAACGCTCACTGGT‑GC‑CCCCCC‑GGGCTTGCCAGGAAACTTAACACCGGTTTGGTCCGTTTGACTATCAGTCCCGCCTCT                                      >  SRR4302543.4859783/1‑94 (MQ=60)

                                                           CCACCATAATTGACGAACGCTCACTGGT‑GC‑CCCCCC‑GGGCTTGCCAGGAAACTTAACACCGGTTTGGTCCGTTTGACTATCAGTCCCGCCTCTT                                     >  SRR4302543.3063676/1‑94 (MQ=60)

                                                            CACCATAATTGACGAACGCTCACTGGG‑GC‑CCCCCC‑GGGCTTGCCAGGAAACTTAACACCGGTTTGGTCCGTTTGACTATCAGTCCCGCCTCTTC                                    >  SRR4302543.5687108/1‑94 (MQ=60)

                                                             ACCATAATTGACGAACGCTCACTGGT‑GC‑CCCCCC‑GGGCTTGCCAGGAAACTTAACACCGGTTTGGTCCGTTTGACTATCAGTCCCGCCTCTTCA                                   >  SRR4302543.3983481/1‑94 (MQ=60)

                                                             ACCATAATTGACGAACGCTCACTGGT‑GC‑CCCCCC‑GGGCTTGCCAGGAAACTTAACACCGGTTTGGTCCGTTTGACTATCAGTCCCGCCTCTTCA                                   >  SRR4302543.7290356/1‑94 (MQ=60)

                                                              CCATAATTGACGAACGCTCACTGGT‑GC‑CCCCCC‑GGGCTTGCCAGGAAACTTAACACCGGTTTGGTCCGTTTGACTATCAGTCCCGCCTCTTCAA                                  >  SRR4302543.2553402/1‑94 (MQ=60)

                                                                 TAATTGACGAACGCTCACTGGT‑GC‑CCCCCC‑GGGCTTGCCAGGAAACTTAACACCGGTTTGGTCCGTTTGACTATCAGTCCCGCCTCTTCAATCG                               >  SRR4302543.7136883/1‑94 (MQ=60)

                                                                   ATTGACGAACGCTCACTGGT‑GC‑CCCCCC‑GGGCTTGCCAGGAAACTTAACACCGGTTTGGTCCGTTTGACTATCAGTCCCGCCTCTTCAATCGCT                             >  SRR4302543.3738226/1‑94 (MQ=60)

                                                                   ATTGACGAACGCTCACTGGT‑GC‑CCCCCC‑GGGCTTGCCAGGAAACTTAACACCGGTTTGGTCCGTTTGACTATCAGTCCCGCCTCTTCAATCGCT                             >  SRR4302543.955369/1‑94 (MQ=60)

                                                                    TTGACGAACGCTCACTGGT‑GC‑CCCCCC‑GGGCTTGCCAGGAAACTTAACACCGGTTTGGTCCGTTTGACTATCAGTCCCGCCTCTTCAATCGCTT                            <  SRR4302543.2808003/94‑1 (MQ=60)

                                                                     TGCCGACCGCTCACTGGT‑GC‑CCCCCC‑GGGCTTGCCAGGAAACTTAACACCGGTTTGGTCCGTTTGACTATCAGTCCCGCCTCTTCAATCGCTTC                           <  SRR4302543.3372311/94‑1 (MQ=60)

                                                                     TGACGAACGCTCACTGGT‑GC‑CCCCCC‑GGGCTTGCCAGGAAACTTAACACCGGTTTGGTCCGTTTGACTATCAGTCCCGCCTCTTCAATCGCTTC                           >  SRR4302543.4808134/1‑94 (MQ=60)

                                                                     TGACGAACGCTCACTGGT‑GC‑CCCCCC‑GGGCTTGCCAGGAAACTTAACACCGGTTTGGTCCGTTTGACTATCAGTCCCGCCTCTTCAATCGCTTC                           <  SRR4302543.458455/94‑1 (MQ=60)

                                                                      GACGAACGCTCACTGGG‑GC‑CCCCCC‑GGGCTTGCCAGGAAACTTAACACCGGTTTGGTCCGTTTGACTATCAGTCCCGCCTCTTCAATCGCTTCG                          <  SRR4302543.585562/94‑1 (MQ=60)

                                                                      GACGAACGCTCACGGGT‑GC‑CCCCCC‑GGGCTTGCCAGGAAACTTAACACCGGTTTGGTCCGTTTGACTATCAGTCCCGCCTCTTCAATCGCTTCG                          <  SRR4302543.4122849/94‑1 (MQ=60)

                                                                       ACGAACGCTCACTGGT‑GCCCCCCCC‑GGGCTTGCCAGGAAACTTAACACCGGTTTGGTCCGTTTGACTATCAGTCCCGCCTCTTCAATCGCTTCG                          >  SRR4302543.2498518/1‑94 (MQ=60)

                                                                       ACGAACGCTCACTGGT‑GCCCCCCCC‑GGGCTTGCCAGGAAACTTAACACCGGTTTGGTCCGTTTGACTATCAGTCCCGCCTCTTCAATCGCTTCG                          <  SRR4302543.4035219/94‑1 (MQ=60)

                                                                       ACGAACGCTCACTGGT‑GC‑CCCCCC‑GGGCTTGCCAGGAAACTTAACACCGGTTTGGTCCGTTTGACTATCAGTCCCGCCTCTTCAATCGCTTCGC                         <  SRR4302543.5763803/94‑1 (MQ=60)

                                                                          ACCGCTCGCTGGT‑G‑‑CCCCCCGGGGCTTGCGAGGAAACTTAGCGCGGGTTTGGTCCGTTTGACTATCAGTCCCGCCTCTTCAATCGCTTCGCGAC                      <  SRR4302543.3346757/94‑1 (MQ=60)

                                                                          AACGCTCACTGGT‑GC‑CCCCCC‑GGGCTTGCCAGGAAACTTAACACCGGTTTGGTCCGTTTGACTATCAGTCCCGCCTCTTCAATCGCTTCGCGAC                      <  SRR4302543.2926400/94‑1 (MQ=60)

                                                                            CGCTCACTGGT‑GC‑CCCCCC‑GGGCTTGCCAGGAAACTTAACACCGGTTTGGTCCGTTTGACTATCAGTCCCGCCTCTTCAATCGCTTCGCGACGG                    <  SRR4302543.7709796/94‑1 (MQ=60)

                                                                            CGCTCACTGGT‑GC‑CCCCCC‑GGGCTTGCCAGGAAACTTAACACCGGTTTGGTCCGTTTGACTATCAGTCCCGCCTCTTCAATCGCTTCGCGACGG                    >  SRR4302543.3971210/1‑94 (MQ=60)

                                                                              CTCACTGGT‑GC‑CCCCCC‑GGGCTTGCCAGGAAACTTAACACCGGTTTGGTCCGTTTGACTATCAGTCCCGCCTCTTCAATCGCTTCGAGACGGGC                  >  SRR4302543.823270/1‑94 (MQ=60)

                                                                               TCACTGGT‑GC‑CCCCCC‑GGGTTTGCCAGGAACCTTAACACCGGTTTGGTCCGTTTGACTATCAGTCCCGCCTCTTCAATCGCTTCGCGACGGGCA                 <  SRR4302543.1291872/94‑1 (MQ=60)

                                                                               TCACTGGT‑GC‑CCCCCC‑GGGCTTGCCAGGAAACTTAACACCGGTTTGGTCCGTTTGACTATCAGTCCCGCCTCTTCAATCGCTTCGCGACGGGCA                 >  SRR4302543.1757555/1‑94 (MQ=60)

                                                                                CACTGGT‑GC‑CCCCCC‑GGGCTTGCCAGGAAACTTAACACCGGTTTGGTCCGTTTGACTATCAGTCCCGCCTCTTCAATCGCTTCGCGACGGGCAA                >  SRR4302543.1682686/1‑94 (MQ=60)

                                                                                 ACTGGT‑GCCCCCCCC‑GGGCTTGCCAGGAAACTTAACACCGGTTTGGTCCGTTTGACTATCAGTCCCGCCTCTTCAATCGCTTCGCGACGGGCAA                >  SRR4302543.2270548/1‑94 (MQ=60)

                                                                                 ACTGGT‑GC‑CCCCCC‑GGGCTTGCCAGGAAACTTAACACCGGTTTGGTCCGTTTGACTATCAGTCCCGCCTCTTCAATCGCTTCGCGACGGGCAAC               >  SRR4302543.7319298/1‑94 (MQ=60)

                                                                                 ACTGGT‑GC‑CCCCCC‑GGGCTTGCCAGGAAACTTAACACCGGTTTGGTCCGTTTGACTATCAGTCCCGCCTCTTCAATCGCTTCGCGACGGGCAAC               >  SRR4302543.5604/1‑94 (MQ=60)

                                                                                 ACTGGT‑GC‑CCCCCC‑GGGCTTGCCAGGAAACTTAACACCGGTTTGGTCCGTTTGACTATCAGTCCCGCCTCTTCAATCGCTTCGCGACGGGCAAC               >  SRR4302543.5602/1‑94 (MQ=60)

                                                                                   TGGT‑GC‑CCCCCC‑GGGCTTGCCAGGAAACTTAACACCGGTTTGGTCCGTTTGACTATCAGTCCCGCCTCTTCAATCGCTTCGCGACGGGCAACAT             >  SRR4302543.678710/1‑94 (MQ=60)

                                                                                   TGGT‑GC‑CCCCCC‑GGGCTTGCCAGGAAACTTAACACCGGTTTGGTCCGTTTGACTATCAGTCCCGCCTCTTCAATCGCTTCGCGACGGGCAACAT             <  SRR4302543.5935447/94‑1 (MQ=60)

                                                                                    GGT‑GC‑CCCCCC‑GGCCTTGCCAGGAAACTTAACACCGGTTTGGTCGGTTTGACTATCAGTCCCGCCTCTTCAATCGCTTCGCGACGGGCAACATC            <  SRR4302543.7168901/94‑1 (MQ=60)

                                                                                     tggtccCCCCCC‑GGGCTTGCCAGGAAACTTAACACCGGTTTGGTCCGTTTGACTATCAGTCCCGCCTCTTCAATCGCTTCGCGACGGGCAACAT             <  SRR4302543.2968623/88‑1 (MQ=60)

                                                                                     cggcgcCCCCCC‑GGGCTTGCCGGGAAACTTAACACCGGTTGGTTCCGTTTGACTATCAGTCCCGCCTCTTCAATCGCTTCGCGACGGGCAACAT             <  SRR4302543.6617963/88‑1 (MQ=60)

                                                                                     aggtccCCCCCC‑GGGCTTGCGAGGAAACTTACCACGGGTTTGGGCCTTTTGACTAACAGTCCCGCCTCTTCAATCGCTTCGCGACGGGCAACAT             <  SRR4302543.5935446/88‑1 (MQ=60)

                                                                                     GT‑GC‑CCCCCC‑GGGCTTGCCAGGAACCTTAACACGGGTTTGGTCCGTTTGCCTATCAGTCCCGCCTCTTCAATCGCTTCGCGACGGGCAACATCT           <  SRR4302543.4951662/94‑1 (MQ=60)

                                                                                     GT‑GC‑CCCCCC‑GGGCTTGCCAGGAAACTTAACACCGGTTTGGTCCGTTTGACTATCAGTCCCGCCTCTTCAATCGCTTCGCGACGGGCAACATCT           <  SRR4302543.2215681/94‑1 (MQ=60)

                                                                                      T‑G‑‑CCCCCCGGGGCTTGCCAGAAAACTTAACCCCGGTTTGGTCCGTTTGACTATCAGTCCCGCCTCTTCAATCGCTTCGCGACGGGCAACATCTT          <  SRR4302543.6969877/94‑1 (MQ=60)

                                                                                       gtccCCCCCC‑GGGCTTTCCCAGAAACCTAACACCGGTTTGGTCCGTTTGACTATCAGTCCCGCCTCTTCAATCGCTTCGCGACGGGCAACATCT           <  SRR4302543.2215680/90‑1 (MQ=60)

                                                                                        gccCCCCCC‑GGGCTTGCCAGGAAACTTAACACCGGTTTGGGCCGTTTGACTATCAGTCCCGCCTCTTCAATCGCTTCGCGACGGGCAACATCTT          <  SRR4302543.6969878/91‑1 (MQ=60)

                                                                                        C‑‑CCCCCC‑GGGCTTGCCAGGAAACTTAACACCGGTTTGGTCCGTTTGACTATCAGTCCCGCCTCTTCAATCGCTTCGCGACGGGCAACATCTTCC        >  SRR4302543.6260108/1‑94 (MQ=60)

                                                                                         gcCCCCCC‑GGGCTTGCCAGAAAACTTAACACCGGTTTGGTCCGTTTGACTATCAGTCCCGCCTCTTCAATCGCTTCGCGACGGGCAACATCTTC         <  SRR4302543.7706024/92‑1 (MQ=60)

                                                                                            CCCCC‑GGGCTTGCCAGGAAACTTAACACCGGTTTGGTCCGTTTGACTATCAGTCCCGCCTCTTCAATCGCTTCGCGACGGGCAACATCTTCCAC      >  SRR4302543.6650729/1‑94 (MQ=60)

                                                                                            CCCCC‑GGGCTTGCCAGGAAACTTAACACCGGTTTGGTCCGTTTGACTATCAGTCCCGCCTCTTCAATCGCTTCGCGACGGGCAACATCTTCCAC      <  SRR4302543.2650419/94‑1 (MQ=60)

                                                                                             CCCC‑GGGCTTGCCAGGAAACTTAACACCGGTTTGGTCCGTTTGACTATCAGTCCCGCCTCTTCAATCGCTTCGCGACGGGCAACATCTTCCACA     >  SRR4302543.5836490/1‑94 (MQ=60)

                                                                                             CCCC‑GGGCTTGCCAGGAAACTTAACACCGGTTTGGTCCGTTTGACTATCAGTCCCGCCTCTTCAATCGCTTCGCGACGGGCAACATCTTCCACA     >  SRR4302543.7658803/1‑94 (MQ=60)

                                                                                              CCC‑GGGCTTGCCAGGAAACTTAACACCGGTTTGGTCCGTTTGACTATCAGTCCCGCCTCTTCAATCGCTTCGCGACGGGCAACATCTTCCACAC    <  SRR4302543.4018669/94‑1 (MQ=60)

                                                                                              CCC‑GGGCTTGCCAGGAAACTTAACACCGGTTTGGTCCGTTTGACTATCAGTCCCGCCTCTTCAATCGCTTCGCGACGGGCAACATCTTCCACAC    >  SRR4302543.3043750/1‑94 (MQ=60)

                                                                                               CC‑GGGCTTGCCAGGAAACTTAACCCCGGTTTGGGCCGTTTGACTATCAGTCCCGCCTCTTCAATCGCTTCGCGACGGGCAACATCTTCCACACT   <  SRR4302543.460877/94‑1 (MQ=60)

                                                                                               CC‑GGGCTTGCCAGGAAACTTAACACCGGTTTGGTCCGTTTGACTATCAGTCCCGCCTCTTCAATCGCTTCGCGACGGGCAACATCTTCCACACT   >  SRR4302543.239373/1‑94 (MQ=60)

                                                                                                C‑GGGCTTTCCAGGAAACTTAACACCGGTTTGGTCCGTTTGACTATCAGTCCCGCCTCTTCAATCGCTTCGCGACGGGCAACATCTTCCACACTT  <  SRR4302543.6284538/94‑1 (MQ=60)

                                                                                                C‑GGGCTTGCCAGGAAACTTAACACCGTTTTGGCCGGTTTTACTATCAGTCCCGCCTCTTCAATCGCTTCGCGACGGGCAACATCTTCCACACTT  <  SRR4302543.1293959/94‑1 (MQ=60)

                                                                                                C‑GGGCTTGCCAGGAAACTTAACACCGGTTTGGTCCGTTTGACTATCAGTCCCGCCTCTTCAATCGCTTCGCGACGGGCAACATCTTCCACACTT  >  SRR4302543.5167708/1‑94 (MQ=60)

                                                                                                C‑GGGCTTGCCAGGAAACTTAACACCGGTTTGGTCCGTTTGACTATCAGTCCCGCCTCTTCAATCGCTTCGCGACGGGCAACATCTTCCACACTT  <  SRR4302543.6284540/94‑1 (MQ=60)

ATCTTCGTTTTCATCAGCCAGACCGTGAATACCGCTTGCGGTCGTGGCGTCCACTTCGCCCACCATAATTGACGAACGCTCACTGGT‑G‑‑CCCCCC‑GGGCTTGCCAGGAAACTTAACACCGGTTTGGTCCGTTTGACTATCAGTCCCGCCTCTTCAATCGCTTCGCGACGGGCAACATCTTCCACACTT  >  NC_000913/3175432‑3175618

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 2 ≤` `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 31 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`