Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I2 R1	1873	183.1	9376148	98.2%	9207377	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,175,525	(C)_6→7	coding (402/630 nt)	yqiE ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,175,519	1	.	C	92.5%	463.7 / 31.7	160	coding (408/630 nt)	yqiE	hypothetical protein
Reads supporting (aligned to +/- strand): ref base . (4/8); new base C (67/80); total (72/88)
Fisher's exact test for biased strand distribution p-value = 5.50e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.83e-01

Only 100 of 189 total aligned reads displayed.

GAATATCTTCGTTTTCATCAGCCAGACCGTGAATACCGCTTGCGGTCGTGGCGTCCACTTCGCCCACCATAATTGACGAACGCTCACTGGT‑G‑CCCCC‑CGGGCTTGCCAGGAAACTTAACACCGGTTTGGTCCGTTTGACTATCAGTCCCGCCTCTTCAATCGCTTCGCGACGGGCAACA  >  NC_000913/3175428‑3175606

   tctCTTCGTTTTCATCAGCCAGACCGTGAATACCGCTTGCGGTCTTGGCGTCCACTTCGCCCACCATAATTGACGAACGCTCACTGGT‑G‑cccc                                                                                      <  2:4235167/91‑1 (MQ=255)

   tatCTTCTTTTTCACCAGCCAGACCGTGAATACCGCTTGCGGTCGTGGCGTCCACTTCGCCCACCATAATTGACGAACGCTCACTGGT‑G‑cccc                                                                                      <  2:2487000/93‑1 (MQ=255)

   tatCTTCGTTTTCATCAGCCAGACCGTGAATACCGCTTGCGGTCGTGGCGTCCACTTCGCCCACCATAATTGACGAACGCTCACTGGT‑G‑cccc                                                                                      >  1:4202206/1‑93 (MQ=255)

   tatCTTCGTTTTCATCAGCCAGACCGTGAATACCGCTTGCGGTCGTGGCGTCCACTTCGCCCACAATAATTGACGAACGCTCACTGGT‑G‑cccc                                                                                      >  1:1008051/1‑93 (MQ=255)

    atCTTCGTTTTCATCAGCCAGACCGTGAATACCGCTTGCGGTCGTGGCGTCCACTTCGCCCACCATAATTGACGAACGCTCACTGGT‑G‑ccccc                                                                                     >  1:2640044/1‑93 (MQ=255)

    atCTTCGTTTTCATCAGCCAGACCGTGAATACCGCTTGCGGTCGTGGCGTCCACTTCGCCCACCATAATTGACGAACGCTCACTGGT‑G‑ccccc                                                                                     >  2:1435799/1‑93 (MQ=255)

     tCTTCGTTTTCATCAGCCAGCCCGTGAATCCCGCTTGCGGTCGTGGCGTCCACTTCGCCCACCATAATTGACGAACGCTCACTGGT‑G‑ccccc‑c                                                                                   <  2:4446644/93‑1 (MQ=255)

     tCTTCGTTTTCATCAGCCAGCCCGTGAATACCGCTTGCGGTCGTGGCGTCCCCTTCGCCCACCATAATTGACGAACGCTCACTGGT‑G‑ccccc‑c                                                                                   <  2:1762207/93‑1 (MQ=255)

     tCTTCGTTTTCATCAGCCAGACCGTGAATCCCGCTTGCGGTCGTGGCGTCCACTTCGCCCACCATAATTGACGAACGCTCACTGGT‑G‑ccccc‑c                                                                                   <  2:462683/93‑1 (MQ=255)

     tCTTCGTTTTCATCAGCCAGACCGTGAATACCGCTTGCGGTCGTGGCGTCCACTTCGCCCACCATAATTGACGAACGCTCACTGGT‑G‑ccccc‑c                                                                                   >  1:3592131/1‑93 (MQ=255)

     tCTTCGTTTTCATCAGCCAGACCGTGAATACCGCTTGCGGTCGTGGCGTCCACTTCGCCCACCATAATTGACGAACGCTCACTGGT‑G‑ccccc‑c                                                                                   >  1:4213438/1‑93 (MQ=255)

      tttCGTTTTCATCAGCCAGACCGTGAATACCGCTTGCGGTCGTGGCGTCCTCTCCGCCCACCATAATTGACGAACGCTCACTGGT‑G‑ccccc‑cc                                                                                  <  2:3501783/92‑2 (MQ=255)

       ttCTTTTGCATCAGCCGGACCGTGAATCCCGCTTGCGGTCGTGGCGTCCACTTCGCCCACCATAATTGACGAACGCTCACTGGT‑G‑ccccc‑ccg                                                                                 <  1:4444684/93‑3 (MQ=255)

        tCGTTTTCATCAGCCAGACCGTGAATCCCGCTTGCGGTCGTGGCGTCCCCTCCGCCCACCATAATTGACGAACGCTCACTGGT‑G‑CCCCC‑Ccgg                                                                                <  2:1528486/93‑1 (MQ=255)

        tCGTTTTCATCAGCCAGACCGTGAATACCGCTTGCGGTCGTGGCGTCCACTTCGCCCACCATAATTGACGAACGCTCACTGGT‑G‑CCCCC‑Ccgg                                                                                <  1:4478989/93‑1 (MQ=255)

        tCGTTTTCATCAGCCAGACCGTGAATACCGCTTGCGGTCGTGGCGTCCACTTCGCCCACCATAATTGACGAACGCTCACTGGT‑G‑CCCCC‑Ccgg                                                                                >  2:4521689/1‑93 (MQ=255)

        tCGTTTTCATCAGCCAGACCGTGAATACCGCTTGCGGTCGTGGCGTCCACTTCGCCCACCATAATTGACGAACGCTCACTGGT‑G‑CCCCC‑Ccgg                                                                                >  2:585318/1‑93 (MQ=255)

        tCGTTTTCATCAGCCAGACCGTGAATACCGCTTGCGGTCGTGGCGTCCACTCCGCCCACCATAATTGACGAACGCTCACTGGT‑G‑CCCCC‑Ccgg                                                                                <  2:2657898/93‑1 (MQ=255)

          gTTTTCATCAGCCAGACCGTGAATCCCGCTTGCGGTCGTGGCTCCCACTTCGCCCACCATAATTGACGAACGCTCACTGGT‑G‑CCCCC‑Ccgggc                                                                              <  1:2983759/93‑3 (MQ=255)

           ttttCATCAGCCAGCCGGTGAATCCCGCTTGCGGTCGTGGCGTCCACTTCGCCCACCATAATTGACGAACGCTCACTGGT‑GCCCCCC‑CGGGCt                                                                              <  2:1788918/93‑1 (MQ=255)

           ttttCATCAGCCAGACCGTGAATACCGCTTGCGGTCGTGGCGTCCACTTCGCCCACCATAATTGACGAACGCTCACTGGT‑GCCCCCC‑CGGGCt                                                                              >  1:4429624/1‑93 (MQ=255)

              tcatcaGCCAGACCGTGAATACCGCTTGCGGTCGTGGCGTCCACTTCGCCCACCATAATTGACGAACGCTCACTGGT‑GCCCCCC‑CGGGCTTGc                                                                           >  1:1467217/1‑93 (MQ=255)

               catcaGCCAAACCTGAAATACCGCTTGGGTTCGTGGCGTCCACTTCGCCCACCATAATTGACGAACGCTCACTGGT‑G‑CCCCCGCGGGCTTGcc                                                                          <  2:2869650/93‑1 (MQ=255)

                atcaGCCAGACCGTGAATACCGCTTGCGGTCGTGGCGTCCACTTCGCCCACCATAATTGACGAACGCTCACTGGT‑GCCCCCC‑CGGGCTTGCCa                                                                         <  1:1491657/93‑1 (MQ=255)

                atcaGCCAGACCGTGAATACCGCTTGCGGTCGTGGCGTCCACTTCGCCCACCATAATTGACGAACGCTCACTGGT‑GCCCCCC‑CGGGCTTGCCa                                                                         >  1:1346020/1‑93 (MQ=255)

                 tcaGCCAGACCGTGAATACCGCTTGCGGTCGTGGCGTCCACTTCGCCCACCATAATTGACGAACGCTCACTGGT‑GCCCCCC‑CGGGCTTGCCAg                                                                        >  1:2783493/1‑93 (MQ=255)

                 tcaCCCAGCCGGTGAATACCGCTTGCGGTCGTGGCGTCCCCTTCGCCCACCATAATTGACGAACGCTCACTGGT‑GCCCCCC‑CGGGCTTGCCAg                                                                        <  1:3441169/93‑1 (MQ=255)

                    gCCAGACCGTGAATACCGCTTGCGGTCGTGGCGTCCACTTCGCCCACCATAATTGACGAACGCTCACTGGT‑GCCCCCC‑CGGGCTTGCCAGGaa                                                                     <  1:4643446/93‑1 (MQ=255)

                    accAGCCCGTGAAGCCCGCTTGCGGTCGTGGCGTCCCCTTCGCCCACCATAATTGACGAACGCTCACTGGT‑GCCCCCC‑CGGGCTTGCCAGGaa                                                                     <  2:2116582/92‑1 (MQ=255)

                      cAGACCGTGAATCCCGCTTGCGGTCGTGGCGTCCACTTCGCCCACCATAATTGACGAACGCTCACTGGT‑GCCCCCC‑CGGGCTTGCCAGGAAAc                                                                   <  1:1689270/93‑1 (MQ=255)

                      cAGACCGTGAATACCGCTTGCGGTCGTGGCGTCCACTTCGCCCACCATAATTGACGAACGCTCACTGGT‑GCCCCCC‑CGGGCTTGCCAGGAAAc                                                                   >  2:2793293/1‑93 (MQ=255)

                      cAGACCGTGAATACCGCTTGCGGTCGTGGCGCCCACTTCGCCCACCATAATTGACGAACGCTCACTGGT‑GCCCCCC‑CGGGCTTGCCAGGAAAc                                                                   <  1:787058/93‑1 (MQ=255)

                       aGACGGTGAATCCCGTTGGCGGTCGTGGCGTCCCCTTCGCCCACCATAATTGACGAACGCTCACTGGT‑GCCCCCC‑CGGGCTTGCCAGGAAACt                                                                  <  2:2691991/93‑1 (MQ=255)

                       aGACCGTGAATCCCGTTTGCGGTCGTGGCGTCCACTTCGCCCACCATAATTGACGAACGCTCACTGGT‑GCCCCCC‑CGGGCTTGCCAGGAAACt                                                                  <  1:2312760/93‑1 (MQ=255)

                        gACCGTGAATACCGCTTGCGGTCGTGGCGTCCACTTCGCCCACCATAATTGACGAACGCTCACTGGT‑GCCCCCC‑CGGGCTTGCCAGGAAACtt                                                                 >  1:3172295/1‑93 (MQ=255)

                        gACCGTGAATACCGCTTGCGGTCGTGGCGTCCACTTCGCCCACCATAATTGACGAACGCTCACTGGT‑GCCCCCC‑CGGGCTTGCCAGGAAACtt                                                                 >  2:485748/1‑93 (MQ=255)

                         aCCGTGAATACCGCTTGCGGTCGTGGCGTCCACTTCGCCCACCATAATTGACGAACGCTCACTGGT‑GCCCCCC‑CGGGCTTGCCAGGAAACTTa                                                                >  2:1179775/1‑93 (MQ=255)

                          cggTGAACGCCGCTCGCGGTCGTGGCGGCCACTCCGCCCACCATAATTGACGAACGCTCACTGGT‑GCCCCCC‑CGGGCTTGCCAGGAAACTTaa                                                               <  2:3563112/91‑1 (MQ=255)

                          ccGTGAATACCGCTTGCGGTCGTGGCGTCCACTTCGCCAACCATAATTGACGAACGCTCACTGGT‑GCCCCCC‑CGGGCTTGCCAGGAAACTTaa                                                               <  1:1996729/93‑1 (MQ=255)

                           cGTGAATACCGTTGGCGGTGGTGGCTTCCTCTCCGCCCACCATAATTGACGAACGCTCACTGGT‑GCCCCCC‑CGGGCTTGCCAGGAAACTTAac                                                              <  1:4023340/93‑1 (MQ=255)

                           cGTGAATACCGCTTGCGGTCGTGGCGTCCACTTCGCCCACCATAATTGACGAACGCTCACTGGT‑GCCCCCC‑CGGGCTTGCCAGGAAACTTAac                                                              >  2:1929397/1‑93 (MQ=255)

                           cGTGAATACCGCTTGCGGTCGTGGCGTCCACTTCGCCCACCATAATTGACGAACGCTCACTGGT‑GCCCCCC‑CGGGCTTGCCAGGAAACTTAac                                                              >  1:475379/1‑93 (MQ=255)

                           cGTGAATACCGCTTGCGGTCGTGGCGTCCACTTCGCCCACCATAATTGACGAACGCTCACTGGT‑GCCCCCC‑CGGGCTTGCCAGGAAACTTAac                                                              >  2:3032850/1‑93 (MQ=255)

                            gTGAATACCGCTTGCGGTCGTGGCGTCCACTTCGCCCACCATAATTGACGAACGCTCACTGGT‑GCCCCCC‑CGGGCTTGCCAGGAAACTTAaca                                                             >  2:1035339/1‑93 (MQ=255)

                             tGAATACCGCTTGCGGTCGTGGCGTCCACTTCGCCCACCATAATTGACGAACGCTCACTGGT‑GCCCCCC‑CGGGCTTGCCAGGAAACTTAacac                                                            >  1:1012293/1‑93 (MQ=255)

                              gAATACCGCTTGCGGTCGTGGCGTCCACTTCGCCCACCATAATTGACGAACGCTCACTGGT‑GCCCCCC‑CGGGCTTGCCAGGACACTTAACAcc                                                           >  2:828874/1‑93 (MQ=255)

                              gAATACCGCTTGCGGTCGTGGCGTCCACTTCGCCCACCATAATTGACGAACGCTCACTGGT‑GCCCCCC‑CGGGCTTGCCAGGAAACTTAACAcc                                                           >  2:1082742/1‑93 (MQ=255)

                                aTACCGCTTGCGGTCGTGGCGTCCACTTCGCCCACCATAATTGACGAACGCTCACTGGT‑GCCCCCC‑CGGGCTTGCCAGGAAACTTAACACCgg                                                         >  2:934116/1‑93 (MQ=255)

                                aTACCGCTTGCGGTCGTGGCGTCCACTTCGCCCACCATAATTGACGAACGCTCACTGGT‑GCCCCCC‑CGGGCTTGCCAGGAAACTTAACACCgg                                                         >  1:1837910/1‑93 (MQ=255)

                                 tcccGCTGGCGGTGGTGGCGTCCACTCCGCCCACCATAATTGACGAACGCTCACTGGT‑GCCCCCC‑CGGGCTTGCCAGGAAACTTAACACCGGt                                                        <  2:1838137/91‑1 (MQ=255)

                                  aCCGCTTGCGGTCGTGGCGTCCACTTCGCCCACCATAATTGACGAACGCTCACTGGT‑GCCCCCC‑CGGGCTTGCCAGGAAACTTAACACCGGtt                                                       >  2:1905701/1‑93 (MQ=255)

                                    ggCTTGCGTGCGTGGCGCCCACTTCGCCCACCATAATTGACGAACGCTCACTGGT‑GCCCCCC‑CGGGCTTGCCAGGAAACTTAACACCGGTTTg                                                     <  1:2132764/92‑1 (MQ=255)

                                    cGCTTGCGTGCGTGGCGACCACTTCCCCCACCATAATTGACGAACGCTCACTGGT‑GCCCCCC‑CGGGCTTGCCAGGAAACTTAACACCGGTTTg                                                     <  2:2173736/93‑1 (MQ=255)

                                     ccTTGGGGTCTTGTCGTCCACTTCGCCCACCATAATTGACGAACGCTCACTGGT‑GCCCCCC‑CGGGCTTGCCAGGAAACTTAACACCGGTTTgg                                                    <  1:2222416/92‑1 (MQ=255)

                                       ttGCGGTCGTGGCGTCCACTTCGCCCACCATAATTGACGAACGCTCACTGGT‑GCCCCCC‑CGGGCTTGCCAGGAAACTTAACACCGGTTTGGTc                                                  >  2:2254256/1‑93 (MQ=255)

                                       ttGCGGTCGTGGCGTCCACTTCGCCCACCATAATTGACGAACGCTCACTGGT‑GCCCCCC‑CGGGCTTGCCAGGAAACTTAACACCGGTTTGGTc                                                  >  1:1899701/1‑93 (MQ=255)

                                        tGCGGTCGTGGCGTCCACTTCGCCCACCATAATTGACGAACGCTCACTGGT‑GCCCCCC‑CGGGCTTGCCAGGAAACTTAACACCGGTTTGGTcc                                                 >  1:2472929/1‑93 (MQ=255)

                                             tCGTGGCGTCCACTTCGCCCACCATAATTGACGAACGCTCACTGGT‑GCCCCCC‑CGGGCTTGCCAGGAAACTTAACACCGGTTTGGTCCGTTTg                                            >  2:3827827/1‑93 (MQ=255)

                                             ccGTGGCGCCCACTGCGCCCACCATAATTGACGAACGCTCACTGGT‑GCCCCCC‑CGGGCTTGCCAGGAAACTTAACACCGGTTTGGTCCGTTTg                                            <  1:885416/92‑1 (MQ=255)

                                                 ggCGTCCACTCCGCCCACCATAATTGACGAACGCTCACTGGT‑GCCCCCC‑CGGGCTTGCCAGGAAACTTAACACCGGTTTGGTCCGTTTGACTa                                        <  2:2005356/93‑1 (MQ=255)

                                                   cGTCCACTTCCCCCACCATAATTGACGAACGCGCACTGGT‑GCCCCCC‑CGGGCTTGCCAGGAAACTTAACACCGGTTTGGTCCGTTTGACTATc                                      <  1:1522198/93‑1 (MQ=255)

                                                    gTCCACTTCGCCCACCATAATTGACGAACGCTCACTGGT‑GCCCCCC‑CGGGCTTGCCAGGAAACTTAACACCGGTTTGGTCCGTTTGACTATCa                                     >  2:539802/1‑93 (MQ=255)

                                                    gTCCACTTCGCCCACCATAATTGACGAACGCTCACTGGT‑GCCCCCC‑CGGGCTTGCCAGGAAACTTAACACCGGTTTGGTCCGTTTGACTATCa                                     >  1:659744/1‑93 (MQ=255)

                                                     tCCACTTCGCCCACCATAATTGACGAACGCTCACTGGT‑GCCCCCC‑CGGGCTTGCCAGGAAACTTAACACCGGTTTGGTCCGTTTGACTATCAg                                    >  1:3846556/1‑93 (MQ=255)

                                                      ccccTTCGCCCACCATAATTGACGAACGCTCACTGGT‑GCCCCCC‑CGGGCTTGCCAGGAAACTTAACACCGGTTTGGTCCGTTTGACTATCAGt                                   <  1:2447944/90‑1 (MQ=255)

                                                      ccACTCCCCCCACCATAATTGACGAACGCTCACTGGT‑GCCCCCC‑CGGGCTTGCCAGGAAACTTAACACCGGTTTGGTCCGTTTGACTATCAGt                                   <  1:3377405/93‑1 (MQ=255)

                                                       cACTTCGCCCACCATAATTGACGAACGCTCACTGGT‑GCCCCCC‑CGGGCTTGCCAGGAAACTTAACACCGGTTTGGTCCGTTTGACTATCAGTc                                  <  1:4498921/93‑1 (MQ=255)

                                                         cTTCGCCCACCATAATTGACGAACGCTCACTGGT‑GCCCCCC‑CGGGCTTGCCAGGAAACTTAACACCGGTTTGGTCCGTTTGACTATCAGTccc                                >  2:3229805/1‑93 (MQ=255)

                                                          ctCGCCCACCATAATTGACGAACGCTCACTGGT‑GCCCCCC‑CGGGCTTGCCAGGAAACTTAACACCGGTTTGGTCCGTTTGACTATCAGTCCCg                               <  1:2582484/92‑1 (MQ=255)

                                                             ccccACCATAATTGACGAACGCTCACTGGT‑GCCCCCC‑CGGGCTTGCCAGGAAACTTAACACCGGTTTGGTCCGTTTGACTATCAGTCCCGCct                            <  2:2883740/92‑1 (MQ=255)

                                                              cccACCATAATTGACGAACGCTCACTGGT‑GCCCCCC‑CGGGCTTGCCAGGAAACTTAACACCGGTTTGGTCCGTTTGACTATCAGTCCCGCctc                           >  2:3152288/1‑93 (MQ=255)

                                                              cccACCATAATTGACGAACGCTCACTGGT‑GCCCCCC‑CGGGCTTGCCAGGAAACTTAACACCGGTTTGGTCCGTTTGACTATCAGTCCCGCctc                           >  1:2023583/1‑93 (MQ=255)

                                                               ccaccaTAATTGACTAACGCTCACTGGT‑GCCCCCC‑CGGGCTTGCCAGGAAACTTAACACCGGTTTGGTCCGTTTGACTATCAGTCCCGCctct                          <  1:4553171/93‑1 (MQ=255)

                                                                caccaTAATTGACGAACGCTCACTGGT‑GCCCCCC‑CGGGCTTGCCAGGAAACTTAACACCGGTTTGGTCCGTTTGACTATCAGTCCCGCCTCtt                         >  1:1567163/1‑93 (MQ=255)

                                                                caccaTAATTGACGAACGCTCACTGGT‑GCCCCCC‑CGGGCTTGCCAGGAAACTTAACACCGGTTTGGTCCGTTTGACTATCAGTCCCGCCTCtt                         >  1:964379/1‑93 (MQ=255)

                                                                caccaTAATTGACGAACGCTCACTGGT‑GCCCCCC‑CGGGCTTGCCAGGAAACTTAACACCGGTTTGGTCCGTTTGACTATCAGTCCCGCCTCtt                         >  1:4252039/1‑93 (MQ=255)

                                                                    aTAATTGACGAACGCTCACTGGT‑GCCCCCC‑CGGGCTTGCCAGGAAACTTAACACCGGTTTGGTCCGTTTGACTATCAGTCCCGCCTCTTCAAt                     <  2:3429387/93‑1 (MQ=255)

                                                                     tAATTGACGAACGCTCACTGGT‑GCCCCCC‑CGGGCTTGCCAGGAAACTTAACACCGGTTTGGTCCGTTTGACTATCAGTCCCGCCTCTTCAATc                    <  1:1969254/93‑1 (MQ=255)

                                                                     tAATTGACGAACGCTCACTGGT‑GCCCCCC‑CGGGCTTGCCAGGAAACTTAACACCGGGTTGGTCCGTTTGACTATCCGTCCCGCCTCTTCAATc                    >  1:4053408/1‑93 (MQ=255)

                                                                        ttGACGAACGCTCACTGGT‑GCCCCCC‑CGGGCTTGCCAGGAAACTTAACACCGGTTTGGTCCGTTTGACTATCAGTCCCGCCTCTTCAATCGCt                 <  1:3119357/93‑1 (MQ=255)

                                                                         tGACGAACGCTCACTGGT‑GCCCCCC‑CGGGCTTGCCAGGAAACTTAACCCCGGTTTGGTCCGTTTGACTATCAGTCCCGCCTCTTCAATCGCtt                <  2:4108264/93‑1 (MQ=255)

                                                                         tGACGAACGCTCACTGGT‑GCCCCCC‑CGGGCTTGCCAGGAAACTTAACACCGGTTTGGTCCGTTTGACTATCAGTCCCGCCTCTTCAATCGCtt                <  1:3128933/93‑1 (MQ=255)

                                                                         tGACGAACGCTCACTGGT‑GCCCCCC‑CGGGCTTGCCAGGAAACTTAACACCGGTTTGGTCCGTTTGACTATCAGTCCCGCCTCTTCAATCGCtt                <  2:3642747/93‑1 (MQ=255)

                                                                          gACGAACGCTCACTGGT‑GCCCCCC‑CGGGCTTGCCAGGAAACTTAACACCGGTTTGGTCCGTTTGACTATCAGTCCCGCCTCTTCAATCGCTTc               <  2:2073360/93‑1 (MQ=255)

                                                                            cGAACGCTCACTGGT‑GCCCCCC‑CGGGCTTGCCAGGAAACTTAACACCGGTTTGGTCCGTTTGACTATCAGTCCCGCCTCTTCAATCGCTTcgc             <  1:1827584/93‑1 (MQ=255)

                                                                             gAACGCTCACTGGT‑GCCCCCC‑CGGGCTTGCCAGGAAACTTAACCCCGGTTTGGTCCGTTTGACTATCAGTCCCGCCTCTTCAATCGCTTcgcg            <  2:2288193/93‑1 (MQ=255)

                                                                               aCGCTCACTGGT‑GCCCCCC‑CGGGCTTGCCAGGAAACTTAACACCGGTTTGGTCCGTTTGACTATCAGTCCCGCCTCTTCAATCGCTTCGCGAc          <  2:1353648/93‑1 (MQ=255)

                                                                               aCGCTCACTGGT‑GCCCCCC‑CGGGCTTGCCAGGAAACTTAACACCGGTTTGGTCCGTTTGACTATCAGTCCCGCCTCTTCAATCGCTTCGCGAc          <  1:3643412/93‑1 (MQ=255)

                                                                                cGCTCACTGGT‑GCCCCCC‑CGGGCTTGCCAGGAAACTTAACACCGGTTTGGTCCGTTTGCCTATCAGTCCCGCCTCTTCAATCGCTTCGCGACg         <  1:3263876/93‑1 (MQ=255)

                                                                                 gCTCACTGGT‑GCCCCCC‑CGGGCTTGCCAGGAAACTTAACACCGGTTTGGTCCTTTTGACTATCAGTCCCGCCTCTTCAATCGCTTCGCGACgg        <  1:3723567/93‑1 (MQ=255)

                                                                                 gCTCACTGGT‑GCCCCCC‑CGGGCTTGCCAGGAAACTTAACACCGGTTTGGTCCGTTTGACTATCAGTCCCGCCTCTTCAATCGCTTCGCGACgg        >  1:2913892/1‑93 (MQ=255)

                                                                                  cTCACTGGTCC‑CCCCC‑CGGGCTTGCCAGGAAACTTACCACCGGTTTGGTCCGTTTGACTATCAGTCCCGCCTCTTCAATCGCTTCGCGACggg       <  2:1624959/93‑1 (MQ=255)

                                                                                  cTCACTGGT‑GCCCCCC‑CGGGCTTGCCAGGAAACTTAACACCGGTTTGGTCCGTTTGACTATCAGTCCCGCCTCTTCAATCGCTTCGCGACggg       >  1:43461/1‑93 (MQ=255)

                                                                                   tCACTGGT‑GCCCCCC‑CGGGCTTGCCAGGAAACTTAACACCGGTTTGGTCCGTTTGACTATCAGTCCCGCCTCTTCAATCGCTTCGCGACGGGc      >  2:903947/1‑93 (MQ=255)

                                                                                    cACTGGT‑GCCCCCC‑CGGGCTTGCCAGGAAACTTAACACCGGTTTGGTCCGTTTGACTATCAGTCCCGCCTCTTCAATCGCTTCGCGACGGGCa     >  2:622902/1‑93 (MQ=255)

                                                                                     aCTGGT‑GCCCCCC‑CGGGCTTGCCAGGAAACTTAACACCGGTTTGGTCCGTTTGACTATCAGTCCCGCCTCTTCAATCGCTTCGCGACGGGCaa    <  1:1095450/93‑1 (MQ=255)

                                                                                      cTGGT‑GCCCCCC‑CGGGCTTGCCAGGAAACTTAACACCGGTTTGGTCCGTTTGACTATCAGTCCCGCCTCTTCAATCGCTTCGCGACGGGCAAc   >  1:929967/1‑93 (MQ=255)

                                                                                      cTGGT‑GCCCCCC‑CGGGCTTGCCAGGAAACTTAACACCGGTTTGGTCCGTTTGACTATCAGTCCCGCCTCTTCAATCGCTTCGCGACGGGCAAc   <  1:719190/93‑1 (MQ=255)

                                                                                       tGGT‑GCCCCCC‑CGGGCTTGCCAGGAAACTTAACACCGGTTTGGTCCGTTTGACTATCAGTCCCGCCTCTTCAATCGCTTCGCGACGGGCAACa  <  1:3868787/93‑1 (MQ=255)

                                                                                       tGGT‑GCCCCCC‑CGGGCTTGCCAGGAAACTTAACACCGGTTTGGTCCGTTTGACTATCAGTCCCGCCTCTTCAATCGCTTCGCGACGGGCAACa  <  1:641031/93‑1 (MQ=255)

GAATATCTTCGTTTTCATCAGCCAGACCGTGAATACCGCTTGCGGTCGTGGCGTCCACTTCGCCCACCATAATTGACGAACGCTCACTGGT‑G‑CCCCC‑CGGGCTTGCCAGGAAACTTAACACCGGTTTGGTCCGTTTGACTATCAGTCCCGCCTCTTCAATCGCTTCGCGACGGGCAACA  >  NC_000913/3175428‑3175606

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 16 ≤` `ATCG/ATCG` `< 24 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

ATCTTCGTTTTCATCAGCCAGACCGTGAATACCGCTTGCGGTCGTGGCGTCCACTTCGCCCACCATAATTGACGAACGCTCACTGGT‑G‑CCCCCCGGG‑CTTGCCAGGAAACTTAACACCGGTTTGGTCCGTTTGACTATCAGTCCCGCCTCTTCAATCGCTTCGCGACGGGCAACATCTTCCACACT  >  NC_000913/3175432‑3175617

ATCTTCGTTTTCATCAGCCAGACCGTGAATACCGCTTGCGGTCGTGGCGTCCACTTCGCCCACCATAATTGACGAACGCTCACTGGT‑G‑CCCCCC                                                                                               >  SRR4302535.3617046/1‑94 (MQ=60)

ATCTTCGTTTTCATCAGCCAGACCGTGAATACCGCTTGCGGTCGTGGCGTCCACTTCGCCCACCATAATTGACGAACGCTCACTGGT‑G‑CCCCCC                                                                                               >  SRR4302535.4242274/1‑94 (MQ=60)

   TTCTTTTGCATCAGCCGGACCGTGAATCCCGCTTGCGGTCGTGGCGTCCACTTCGCCCACCATAATTGACGAACGCTCACTGGT‑G‑CCCCCCCGG                                                                                            <  SRR4302535.4475163/94‑1 (MQ=60)

    tctttttacacacccagcccgtaaatccctCTTGCGGTCGTGGCGTCCCCTTCGCCCACCATAATTGACGAACGCTCACTGGT‑GCCCCCCCGGG                                                                                            <  SRR4302535.858283/64‑1 (MQ=60)

    TCGTTTTCATCAGCCAGACCGTGAATACCGCTTGCGGTCGTGGCGTCCACTTCGCCCACCATAATTGACGAACGCTCACTGGT‑GCCCCCCCGGG                                                                                            <  SRR4302535.4509740/94‑1 (MQ=60)

      GTTTTCATCAGCCAGACCGTGAATCCCGCTTGCGGTCGTGGCTCCCACTTCGCCCACCATAATTGACGAACGCTCACTGGT‑GCCCCCCCGGG‑CT                                                                                         <  SRR4302535.3004688/94‑1 (MQ=60)

      GTTTTCATCAGCCAGACCGTGAATACCGCTTGCGGTCGTGGCGTCCACTTCGCCCACCATAATTGACGAACGCTCACTGGT‑GCCCCCCCGGG‑CT                                                                                         >  SRR4302535.4459974/1‑94 (MQ=60)

      GTTTTCATCAGCCAGACCGTGAATACCGCTTGCGGTCGTGGCGTCCACTTCGCCCACCATAATTGACGAACGCTCACTGGT‑GCCCCCCCGGG‑CT                                                                                         >  SRR4302535.3019080/1‑94 (MQ=60)

       TTTTCAACGGCCAGACCGTTAATCCCGTTCGCGGTCGTGGCGTCCACTTCCCCCACCATAATTGACGAACGCTCACTGGT‑GCCCCCCCGGG‑CTT                                                                                        <  SRR4302535.4243176/94‑1 (MQ=60)

         TTCATCAGCCAGACCGTGAATACCGCTTGCGGTCGTGGCGTCCACTTCGCCCACCATAATTGACGAACGCTCACTGGT‑GCCCCCCCGGG‑CTTGC                                                                                      >  SRR4302535.1477726/1‑94 (MQ=60)

           CATCAGCCAGACCGTGAATACCGCTTGCGGTCGTGGCGTCCACTTCGCCCACCATAATTGACGAACGCTCACTGGT‑GCCCCCCCGGG‑CTTGCCA                                                                                    >  SRR4302535.1355631/1‑94 (MQ=60)

            ATCAGCCAGACCGTGAATACCGCTTGCGGTCGTGGCGTCCACTTCGCCCACCATAATTGACGAACGCTCACTGGT‑GCCCCCCCGGG‑CTTGCCAG                                                                                   >  SRR4302535.2803300/1‑94 (MQ=60)

            ATCAGCCAGACCGTGAATACCGCTTGCGGTCGTGGCGTCCACTTCGCCCACCATAATTGACGAACGCTCACTGGT‑GCCCCCCCGGG‑CTTGCCAG                                                                                   <  SRR4302535.1502371/94‑1 (MQ=60)

             TCACCCAGCCGGTGAATACCGCTTGCGGTCGTGGCGTCCCCTTCGCCCACCATAATTGACGAACGCTCACTGGT‑GCCCCCCCGGG‑CTTGCCAGG                                                                                  <  SRR4302535.3465098/94‑1 (MQ=60)

               AGCCAGACCGTGAATACCGCTTGCGGTCGTGGCGTCCACTTCGCCCACCATAATTGACGAACGCTCACTGGC‑GCCCCCCCGGG‑CTTGCCAGGAA                                                                                >  SRR4302535.2007629/1‑94 (MQ=60)

                GCCAGACCGTGAATACCGCTTGCGGTCGTGGCGTCCACTTCGCCCACCATAATTGACGAACGCTCACTGGT‑GCCCCCCCGGG‑CTTGCCAGGAAA                                                                               <  SRR4302535.4674918/94‑1 (MQ=60)

                 CCAGACCGTGAATACCGCTTGCGGTCGTGGCGTCCACTTCGCCCACCATAATTGACGAACGCTCACTGGT‑GCCCCCCCCGG‑CTTGCCAGGAAAC                                                                              >  SRR4302535.3536152/1‑94 (MQ=60)

                  CAGCCCGTAAATCCCGCTTGCGGTCGTGGCGTCCCCTTCGCCCACCATAATTGACGAACGCTCACTGGT‑GCCCCCCCGGG‑CTTGCCAGGAAACT                                                                             <  SRR4302535.3754179/94‑1 (MQ=60)

                  CAGACCGTGAATCCCGCTTGCGGTCGTGGCGTCCACTTCGCCCACCATAATTGACGAACGCTCACTGGT‑GCCCCCCCGGG‑CTTGCCAGGAAACT                                                                             <  SRR4302535.1701495/94‑1 (MQ=60)

                  CAGACCGTGAATACCGCTTGCGGTCGTGGCGCCCACTTCGCCCACCATAATTGACGAACGCTCACTGGT‑GCCCCCCCGGG‑CTTGCCAGGAAACT                                                                             <  SRR4302535.792578/94‑1 (MQ=60)

                   AGCCCGTGAATACCGCTTGCGGTCGTGGCGTCCCCTTCCCCCACCATAATTGACGAACGCTCACTGGT‑GCCCCCCCGGG‑CTTGCCAGGAAACTT                                                                            <  SRR4302535.3400504/94‑1 (MQ=60)

                   AGACCGTGAATCCCGTTTGCGGTCGTGGCGTCCACTTCGCCCACCATAATTGACGAACGCTCACTGGT‑GCCCCCCCGGG‑CTTGCCAGGAAACTT                                                                            <  SRR4302535.2329313/94‑1 (MQ=60)

                   AGACCGTGAATACCGCTTGCGGTCGTGGCGTCCACTTCGCCCACCATAATTGACGAACGCTCACTGGT‑GCCCCCCCGGG‑CTTGCCAGGAAACTT                                                                            >  SRR4302535.3194254/1‑94 (MQ=60)

                   AGACCGTGAATACCGCTTGCGGTCGTGGCGTCCACTTCGCCCACCATAATTGACGAACGCTCACTGGT‑GCCCCCCCGGG‑CTTGCCAGGAAACTT                                                                            >  SRR4302535.3674641/1‑94 (MQ=60)

                      CCGTGAATACCGCTTGCGGTCGTGGCGTCCACTTCGCCCACCATAATTGACGAACGCTCACTGGT‑GCCCCCCCGGG‑CTTGCCAGGAAACTTAAC                                                                         >  SRR4302535.478714/1‑94 (MQ=60)

                      CCGTGAATACCGCTTGCGGTCGTGGCGTCCACTTCGCCAACCATAATTGACGAACGCTCACTGGT‑GCCCCCCCGGG‑CTTGCCAGGAAACTTAAC                                                                         <  SRR4302535.2011103/94‑1 (MQ=60)

                       CGTGAATACCGTTGGCGGTGGTGGCTTCCTCTCCGCCCACCATAATTGACGAACGCTCACTGGT‑GCCCCCCCGGG‑CTTGCCAGGAAACTTAACA                                                                        <  SRR4302535.4050866/94‑1 (MQ=60)

                        GTGAATACCGCTTGCGGTCGTGGCGTCCACTTCGCCCACCATAATTGACGAACGCTCACTGGT‑GCCCCCCCGGG‑CTTGCCAGGAAACTTAACAC                                                                       >  SRR4302535.1019491/1‑94 (MQ=60)

                         TGAATACCGCTTGCGGTCGTGGCGTCCACTTCGCCCACCATAATTGACGAACGCTCACTGGT‑GACCCCCCGGG‑CTTGCCAGGAAACTTAACACC                                                                      >  SRR4302535.1738492/1‑94 (MQ=60)

                          GAATCCCGCTTGCGGTCGTGGCGTCCACTTCGCCCACCATAATTGACGAACGCTCACTGGT‑GCCCCCCCGGG‑CTTGCCAGGAAACTTAACACCG                                                                     <  SRR4302535.4522407/94‑1 (MQ=60)

                           AATACCGCTTGCGGTCGTGGCGTCCACTTCGCCCACCATAATTGACGAACGCTCACTGGT‑GCCCCCCCGGG‑CTTGCCAGGAAACTTAACACCGG                                                                    >  SRR4302535.1851169/1‑94 (MQ=60)

                            ATCCCGCTTGCGGTCGTGGCGTCCACTTCGCCCACCATAATTGACGAACGCTCACTGGT‑GCCCCCCCGGG‑CTTGCCAGGAAACTTAACACCGGT                                                                   <  SRR4302535.2652989/94‑1 (MQ=60)

                              CCCGCTTGCGGTCGTGGCGTCCCCTCCGCCCACCATAATTGACGAACGCTCACTGGT‑GCCCCCCCGGG‑CTTGCCAGGAAACTTAACACCGGTTT                                                                 <  SRR4302535.84853/94‑1 (MQ=60)

                              ACCGCTGGCGTTCGTGGCGCCCACTGCGCCCACCATAATTGACGAACGCTCACTGGT‑GCCCCCCCGGG‑CTTGCCAGGAAACTTAACACCGGTTT                                                                 <  SRR4302535.2305243/94‑1 (MQ=60)

                               CCGCTGGCGGCCGTGTCGCCCACTTCCCCCACCATAATTGACGAACGCTCACTGGT‑GCCCCCCCGGG‑CTTGCCAGGAAACTTAACACCGGTTTG                                                                <  SRR4302535.449947/94‑1 (MQ=60)

                                ggcttgcgtgCGTGGCGCCCACTTCGCCCACCATAATTGACGAACGCTCACTGGT‑GCCCCCCCGGG‑CTTGCCAGGAAACTTAACACCGGTTTGG                                                               <  SRR4302535.2148137/84‑1 (MQ=60)

                                CGCTTGCGGTCGTGGCGTCCACTCCGCCCACCATAATTGACGAACGCTCACTGGT‑GCCCCCCCGGG‑CTTGCCAGGAAACTTAACACCGGTTTGG                                                               <  SRR4302535.1507184/94‑1 (MQ=60)

                                 ccttggggtcttgtCGTCCACTTCGCCCACCATAATTGACGAACGCTCACTGGT‑GCCCCCCCGGG‑CTTGCCAGGAAACTTAACACCGGTTTGGT                                                              <  SRR4302535.2238235/80‑1 (MQ=60)

                                  CTTGCGGTCGTGGCGTCCACTTCGCCCACCATAATTGACGAACGCTCACTGGT‑GCCCCCCCGGG‑CTTGCCAGGAAACTTAACACCGGTTTGGTC                                                             >  SRR4302535.2716535/1‑94 (MQ=60)

                                  CTTGCGGTCGTGGCGTCCACTTCGCCCACCATAATTGACGAACGCTCACTGGT‑GCCCCCCCGGG‑CTTGCCAGGAAACTTAACACCGGTTTGGTC                                                             >  SRR4302535.1913373/1‑94 (MQ=60)

                                   TTGCGGTCGTGGCGTCCACTTCGCCCACCATAATTGACGAACGCTCACTGGT‑GCCCCCCCGGG‑CTTGCCAGGAAACTTAACACCGGTTTGGTCC                                                            >  SRR4302535.2490764/1‑94 (MQ=60)

                                    TGCGGTCGTGGCGTCCACTTCGCCCACCATAATTGACGAACGCTCACTGGT‑GCCCCCCCGGG‑CTTGCCAGGAAACTTAACACCGGTTTGGTCCG                                                           <  SRR4302535.1575286/94‑1 (MQ=60)

                                        GTCGTGGCGTCCACTTCGCCCACCATAATTGACGAACGCTCACTGGT‑GCCCCCCCGGG‑CTTGCCAGGAAACTTAACACCGGTTTGGTCCGTTTG                                                       >  SRR4302535.919832/1‑94 (MQ=60)

                                         CCGTGGCGCCCACTGCGCCCACCATAATTGACGAACGCTCACTGGT‑GCCCCCCCGGG‑CTTGCCAGGAAACTTAACACCGGTTTGGTCCGTTTGA                                                      <  SRR4302535.891681/94‑1 (MQ=60)

                                          CGTGGCGTCCACTTCGCCCACCATAATTGACGAACGCTCACTGGT‑GCCCCCCCGGG‑CTTGCCAGGAAACTTAACACCGGTTTGGTCCGTTTGAC                                                     >  SRR4302535.4459575/1‑94 (MQ=60)

                                           gtggcttcccctcccCCCACCATAATTGACGAACGCTCACTGGT‑GCCCCCCCGGG‑CTTGCCAGGAAACTTAACACCGGTTTGGTCCGTTTGACT                                                    <  SRR4302535.4014045/79‑1 (MQ=60)

                                           GTGGCGTCCACTTCGCCCACCATAATTGACGAACGCTCACTGGT‑GCCCCCCCGGG‑CTTGCCAGGAAACTTAACACCGGTTTGGTCCGTTTGACT                                                    >  SRR4302535.1488323/1‑94 (MQ=60)

                                           GTGGCGTCCACTTCGCCCACCATAATTGACGAACGCTCACTGGT‑GCCCCCCCGGG‑CTTGCCAGGAAACTTAACACCGGTTTGGTCCGTTTGACT                                                    >  SRR4302535.3892369/1‑94 (MQ=60)

                                            gggcctccgCTTCGCCCACCATAATTGACGAACGCTCACTGGT‑GCCCCCCCGGG‑CTTGCCAGGAAACTTAACACCGGTTTGGTCCGTTTGACTA                                                   <  SRR4302535.671276/85‑1 (MQ=60)

                                             GGCGCCCACTTCGCCCACCATAATTGACGAACGCTCACTGGT‑GCCCCCCCGGG‑CTTGCCAGGAAACTTAACACCGGTTTGGTCCGTTTGACTAT                                                  <  SRR4302535.3180945/94‑1 (MQ=60)

                                              gcccccccttccCCCACCATAATTGACGAACGCTCACTGGT‑GCCCCCCCGGG‑CTTGCCAGGAAACTTAACACCGGTTTGGTCCGTTTGACTATC                                                 <  SRR4302535.3367245/82‑1 (MQ=60)

                                               CGTCCACTTCGCCCACCATAATTGACGAACGCTCACTGGT‑GCCCCCCCGGG‑CTTGCCAGGAAACTTAACACCGGTTTGGTCCGTTTGACTATCA                                                >  SRR4302535.1939071/1‑94 (MQ=60)

                                               CGTCCACTTCGCCCACCATAATTGACGAACGCTCACTGGT‑GCCCCCCCGGG‑CTTGCCAGGAAACTTAACACCGGTTTGGTCCGTTTGACTATCA                                                >  SRR4302535.664347/1‑94 (MQ=60)

                                               CGTCCACTTCCCCCACCATAATTGACGAACGCGCACTGGT‑GCCCCCCCGGG‑CTTGCCAGGAAACTTAACACCGGTTTGGTCCGTTTGACTATCA                                                <  SRR4302535.1533161/94‑1 (MQ=60)

                                                GTCCACTTCGCCCACCATAATTGACGAACGCTCACTGGT‑GCCCCCCCGGG‑CTTGCCAGGAAACTTAACACCGGTTTGGTCCGTTTGACTATCAG                                               >  SRR4302535.3873038/1‑94 (MQ=60)

                                                 TCCACTTCCCCCACCATAATTGACGAACGCTCACTGGT‑GCCCCCCCGGG‑CTTGCCAGGAAACTTAACACCGGTTTGGTCCGTTTGACTATCAGT                                              <  SRR4302535.4123105/94‑1 (MQ=60)

                                                  CCCCTTCGCCCACCATAATTGACGAACGCTCACTGGT‑GCCCCCCCGGG‑CTTGCCAGGAAACTTAACACCGGTTTGGTCCGTTTGACTATCAGTC                                             <  SRR4302535.2465582/94‑1 (MQ=60)

                                                  CCACTTCGCCCACCATAATTGACGAACGCTCACTGGT‑GCCCCCCCGGG‑CTTGCCAGGAAACTTAACACCGGTTTGGTCCGTTTGACTATCAGTC                                             >  SRR4302535.2446125/1‑94 (MQ=60)

                                                  CCACTCCCCCCACCATAATTGACGAACGCTCACTGGT‑GCCCCCCCGGG‑CTTGCCAGGAAACTTAACACCGGTTTGGTCCGTTTGACTATCAGTC                                             <  SRR4302535.3400889/94‑1 (MQ=60)

                                                   CACTTCGCCCACCATAATTGACGAACGCTCACTGGT‑GCCCCCCCGGG‑CTTGCCAGGAAACTTAACACCGGTTTGGTCCGTTTGACTATCAGTCC                                            >  SRR4302535.1391928/1‑94 (MQ=60)

                                                   CACTTCGCCCACCATAATTGACGAACGCTCACTGGT‑GCCCCCCCGGG‑CTTGCCAGGAAACTTAACACCGGTTTGGTCCGTTTGACTATCAGTCC                                            <  SRR4302535.4529810/94‑1 (MQ=60)

                                                    ctTTCGCCCACCATAATTGACGAACGCTCACTGGT‑GCCCCCCCGGG‑CTTGCCAGGAAACTTAACACCGGTTTGGTCCGTTTGACTATCAGTCCC                                           <  SRR4302535.299738/92‑1 (MQ=60)

                                                    ACTTCGCCCACCATAATTGACGAACGCTCACTGGT‑GCCCCCCCGGG‑CTTGCCAGGAAACTTAACACCGGTTTGGTCCGTTTGACTATCAGTCCC                                           >  SRR4302535.3645931/1‑94 (MQ=60)

                                                     ctcccCCCACCATAATTGACGAACGCTCACTGGT‑GCCCCCCCGGG‑CTTGCCAGGAAACTTAACACCGGTTTGGTCCGTTTGACTATCAGTCCCG                                          <  SRR4302535.446617/89‑1 (MQ=60)

                                                      CTCGCCCACCATAATTGACGAACGCTCACTGGT‑GCCCCCCCGGG‑CTTGCCAGGAAACTTAACACCGGTTTGGTCCGTTTGACTATCAGTCCCGC                                         <  SRR4302535.2601037/94‑1 (MQ=60)

                                                         GCCCACCATAATTGACGAACGCTCACTGGT‑GCCCCCCCGGG‑CTTGCCAGGAAACTTAACACCGGTTTGGTCCGTTTGACTATCAGTCCCGCCTC                                      >  SRR4302535.2038167/1‑94 (MQ=60)

                                                         CCCCACCATAATTGACGAACGCTCACTGGT‑GCCCCCCCGGG‑CTTGCCAGGAAACTTAACACCGGTTTGGTCCGTTTGACTATCAGTCCCGCCTC                                      <  SRR4302535.654101/94‑1 (MQ=60)

                                                           CCACCATAATTGACTAACGCTCACTGGT‑GCCCCCCCGGG‑CTTGCCAGGAAACTTAACACCGGTTTGGTCCGTTTGACTATCAGTCCCGCCTCTT                                    <  SRR4302535.4584282/94‑1 (MQ=60)

                                                           CCACCATAATTGACGAACGCTCACTGGT‑GCCCCCCCGGG‑CTTGCCAGGAAACTTAACACCGGTTTGGTCCGTTTGACTATCAGTCCCGCCTCTT                                    >  SRR4302535.971232/1‑94 (MQ=60)

                                                           CCACCATAATTGACGAACGCTCACTGGT‑GCCCCCCCGGG‑CTTGCCAGGAAACTTAACACCGGTTTGGTCCGTTTGACTATCAGTCCCGCCTCTT                                    >  SRR4302535.4281175/1‑94 (MQ=60)

                                                           CCACCATAATTGACGAACGCTCACTGGT‑GCCCCCCCGGG‑CTTGCCAGGAAACTTAACACCGGTTTGGTCCGTTTGACTATCAGTCCCGCCTCTT                                    >  SRR4302535.4713050/1‑94 (MQ=60)

                                                           CCACCATAATTGACGAACGCTCACTGGT‑GCCCCCCCGGG‑CTTGCCAGGAAACTTAACACCGGTTTGGTCCGTTTGACTATCAGTCCCGCCTCTT                                    >  SRR4302535.1578471/1‑94 (MQ=60)

                                                           CCACCATAATTGACGAACGCTCACTGGT‑GCCCCCCCGGG‑CGTGCCAGGAAACTTAACACCGGTTTGGTCCGTTTGACTATCAGTCCCGCCTCTT                                    >  SRR4302535.3409782/1‑94 (MQ=60)

                                                             CCCATAATTGACGAACGCTCACTGGT‑GCCCCCCCGGG‑CTTGCCAGGAAACTTAACACCGGTTTGGTCCGTTTGACTATCAGTCCCGCCTCTTCA                                  <  SRR4302535.792114/94‑1 (MQ=60)

                                                               CATAATTGACGAACGCTCACTGGT‑GCCCCCCCGGG‑CTTGCCAGGAAACTTAACACCGGTTTGGTCCGTTTGACTATCAGTCCCGCCTCTTCAAT                                <  SRR4302535.1129926/94‑1 (MQ=60)

                                                                ATAATTGACGAACGCTCACTGGT‑GCCCCCCCGGG‑CTTGCCAGGAAACTTAACACCGGGTTGGTCCGTTTGACTATCCGTCCCGCCTCTTCAATC                               >  SRR4302535.4081164/1‑94 (MQ=60)

                                                                 TAATTGCCCAACCCTCTCTGGT‑G‑CCCCCCGGGCCTTGCCAGGAACCTTACCACCGGTTTGGTCCGTTTGACTATCAGTCCCGCCTCTTCAATCG                              <  SRR4302535.207509/94‑1 (MQ=60)

                                                                 TAATTGACGAACGCTCACTGGT‑GCCCCCCCGGG‑CTTGCCAGGAAACTTAACACCGGTTTGGTCCGTTTGACTATCAGTCCCGCCTCTTCAATCG                              <  SRR4302535.2907226/94‑1 (MQ=60)

                                                                 TAATTGACGAACGCTCACTGGT‑GCCCCCCCGGG‑CTTGCCAGGAAACTTAACACCGGTTTGGTCCGTTTGACTATCAGTCCCGCCTCTTCAATCG                              <  SRR4302535.1983441/94‑1 (MQ=60)

                                                                  AATTGACGAACGCTCACTGGT‑GCCCCCCCGGG‑CTTGCCAGGAAACTTAACACCGGTTTGGTCCGTTTGACTATCAGTCCCGCCTCTTCAATCGC                             <  SRR4302535.614650/94‑1 (MQ=60)

                                                                   ATTGACGAACGCTCACTGGT‑GCCCCCCCGGG‑CTTGCCAGGAAACTTAACACCGGTTTGGTCCGTTTGACTATCAGTCCCGCCTCTTCAATCGCT                            >  SRR4302535.4653571/1‑94 (MQ=60)

                                                                   ATTGACGAACGCTCACTGGT‑G‑CCCCCCGGG‑CTTGCCAGGAAACTTAACACCGGTTTGGTCCGGTTGACTATCAGTCCCGCCTCTTCCATCGCTT                           >  SRR4302535.1808229/1‑94 (MQ=60)

                                                                    TTGACGAACGCTCACTGGT‑GCCCCCCCGGG‑CTTGCCAGGAAACTTAACACCGGTTTGGTCCGTTTGACTATCAGTCCCGCCTCTTCAATCGCTT                           <  SRR4302535.3141074/94‑1 (MQ=60)

                                                                     TGACGAACGCTCACTGGT‑GCCCCCCCGGG‑CTTGCCAGGAAACTTAACACCGGTTTGGTCCGTTTGACTATCAGTCCCGCCTCTTCAATCGCTTC                          <  SRR4302535.3150724/94‑1 (MQ=60)

                                                                        CGAACGCTCACTGGTCC‑CCCCCCGGG‑CTTGCCAGGAAACTTAACACCGGTTTGGTCCGTTTGACTATCAGTCCCGCCTCTTCAATCGCTTCGCG                       <  SRR4302535.4293787/94‑1 (MQ=60)

                                                                        CGAACGCTCACTGGT‑GCCCCCCCGGG‑CTTGCCAGGAAACTTAACACCGGTTTGGTCCGTTTGACTATCAGTCCCGCCTCTTCAATCGCTTCGCG                       <  SRR4302535.4239352/94‑1 (MQ=60)

                                                                        CGAACGCTCACTGGT‑GCCCCCCCGGG‑CTTGCCAGGAAACTTAACACCGGTTTGGTCCGTTTGACTATCAGTCCCGCCTCTTCAATCGCTTCGCG                       <  SRR4302535.1840782/94‑1 (MQ=60)

                                                                           ACGCTCACTGGT‑GCCCCCCCGGG‑CTTGCCAGGAAACTTAACACCGGTTTGGTCCGTTTGACTATCAGTCCCGCCTCTTCAATCGCTTCGCGACG                    <  SRR4302535.3668644/94‑1 (MQ=60)

                                                                            CGCTCACTGGT‑GCCCCCCCGGG‑CTTGCCAGGAAACTTAACACCGGTTTGGTCCGTTTGCCTATCAGTCCCGCCTCTTCAATCGCTTCGCGACGG                   <  SRR4302535.3286544/94‑1 (MQ=60)

                                                                            CGCTCACTGGT‑GCCCCCCCGGG‑CTTGCCAGGAAACTTAACACCGGTTTGGTCCGTTTGACTATCAGTCCCGCCTCTTCAATCGCTTCGCGACGG                   >  SRR4302535.2934476/1‑94 (MQ=60)

                                                                            CGCTCACTGGT‑GCCCCCCCGGG‑CTTGCCAGGAAACTTAACACCGGTTTGGTCCGTTTGACTATCAGTCCCGCCTCTTCAATCGCTTCGCGACGG                   <  SRR4302535.2211385/94‑1 (MQ=60)

                                                                             GCTCACTGGT‑GCCCCCCCGGG‑CTTGCCAGGAAACTTAACACCGGTTTGGTCCTTTTGACTATCAGTCCCGCCTCTTCAATCGCTTCGCGACGGG                  <  SRR4302535.3749362/94‑1 (MQ=60)

                                                                             GCTCACTGGT‑GCCCCCCCGGG‑CTTGCCAGGAAACTTAACACCGGTTTGGTCCGTTTGACTATCAGTCCCGCCTCTTCAATCGCTTCGCGACGGG                  >  SRR4302535.43789/1‑94 (MQ=60)

                                                                             GCTCACTGGT‑GCCCCCCCGGG‑CTTGCCAGGAAACTTAACACCGGTTTGGTCCGTTTGACTATCAGTCCCGCCTCTTCAATCGCTTCGCGACGGG                  >  SRR4302535.2052056/1‑94 (MQ=60)

                                                                             GCTCACTGGT‑GCCCCCCCGGG‑CTTGCCAGGAAACTTAACACCGGTTTGGTCCGTTTGACTATCAGTCCCGCCTCTTCAATCGCTTCGCGACGGG                  >  SRR4302535.2017032/1‑94 (MQ=60)

                                                                              CTCACTGGT‑GCCCCCCCGGG‑CTTGCCAGGAAACTTAACACCGGTTTGGTCCGTTTGACTATCAGTCCCGCCTCTTCAATCGCTTCGCGACGGGC                 <  SRR4302535.3143453/94‑1 (MQ=60)

                                                                                CACTGGT‑GCCCCCCCGGG‑CTTGCCAGGAAACTTAACACCGGTTTGGTCCGTTTGACTATCAGTCCCGCCTCTTCAATCGCTTCGCGACGGGCAA               >  SRR4302535.4465920/1‑94 (MQ=60)

                                                                                 ACTGGT‑GCCCCCCCGGG‑CTTGCCAGGAAACTTAACACCGGTTTGGTCCGTTTGACTATCAGTCCCGCCTCTTCAATCGCTTCGCGACGGGCAAC              >  SRR4302535.936545/1‑94 (MQ=60)

                                                                                 ACTGGT‑GCCCCCCCGGG‑CTTGCCAGGAAACTTAACACCGGTTTGGTCCGTTTGACTATCAGTCCCGCCTCTTCAATCGCTTCGCGACGGGCAAC              <  SRR4302535.1103245/94‑1 (MQ=60)

                                                                                  CTGGT‑GCCCCCCCGGG‑CTTGCCAGGAAACTTAACACCGGTTTGGTCCGTTTGACTATCAGTCCCGCCTCTTCAATCGCTTCGCGACGGGCAACA             <  SRR4302535.724244/94‑1 (MQ=60)

                                                                                   cgggtccCCCCCCGGG‑CTTGCCAGGAATCTTAACCCCGTTTTGGCCCGTTTGACTATCAGTCCCGCCTCTTCAATCGCTTCGCGACGGGCAACA             <  SRR4302535.3659300/87‑1 (MQ=60)

                                                                                   TGGT‑GCCCCCCCGGG‑CTTGCCAGGAAACTTAACACCGGTTTGGTCCGTTTGACTATCAGTCCCGCCTCTTCAATCGCTTCGCGACGGGCAACAT            >  SRR4302535.2299147/1‑94 (MQ=60)

                                                                                   TGGT‑GCCCCCCCGGG‑CTTGCCAGGAAACTTAACACCGGTTTGGTCCGTTTGACTATCAGTCCCGCCTCTTCAATCGCTTCGCGACGGGCAACAT            <  SRR4302535.3895381/94‑1 (MQ=60)

                                                                                   TGGT‑GCCCCCCCGGG‑CTTGCCAGGAAACTTAACACCGGTTTGGTCCGTTTGACTATCAGTCCCGCCTCTTCAATCGCTTCGCGACGGGCAACAT            <  SRR4302535.645509/94‑1 (MQ=60)

                                                                                   TGGT‑GCCCCCCCGGG‑CTTGCCAGGAAACTTAACACCGGTTTGGTCCGTTTGACTATCAGTCCCGCCTCTTCAATCGCTTCGCGACGGGCAACAT            <  SRR4302535.1061106/94‑1 (MQ=60)

                                                                                   TGGT‑GCCCCCCCGGG‑CTTGCCAGGAAACTTAACACCGGTTTGGTCCGTTTGACTATCAGTCCCGCCTCTTCAATCGCTTAGCGACGGGCCACAT            >  SRR4302535.4222338/1‑94 (MQ=60)

                                                                                    GGT‑GCCCCCCCGGG‑CTTGCCAGGAAACTTAACACCGGTTTGGTCCGTTTGACTATCAGTCCCGCCTCTTCAATCGCTTCGCGACGGGCAACATC           <  SRR4302535.1125616/94‑1 (MQ=60)

                                                                                    GGT‑GCCCCCCCGGG‑CTTGCCAGGAAACTTAACACCGGTTTGGTCCGTTTGACTATCAGTCCCGCCTCTTCAATCGCTTCGCGACGGGCAACATC           >  SRR4302535.2422489/1‑94 (MQ=60)

                                                                                     GT‑GCCCCCCCGGG‑CTTGCCAGGAAACTTAACACCGGTTTGGTCCGTTTGACTATCAGTCCCGCCTCTTCAATCGCTTCGCGACGGGCAACATCT          <  SRR4302535.1473833/94‑1 (MQ=60)

                                                                                       tgcCCCCCCGGG‑CTTGCCAGGAAACTTAACACCGGTTTGGTCCGTTTGACTATCAGTCCCGCCTCTTCAATCGCTTCGCGACGGGCAACATCTT         <  SRR4302535.4213958/91‑1 (MQ=60)

                                                                                       ggcCCCCCCGGG‑CTTGCCAGGAAACTTAACACCGGTTTGGTCCGTTTGACTATCAGTCCCGCCTCTTCAATCGCTTCGCGACGGGCAACATCTT         <  SRR4302535.3858165/91‑1 (MQ=60)

                                                                                        gcCCCCCCGGG‑CTTGCCAGGAAACTTAACACCGGTTTGGTCCGTTTGACTATCAGTCCCGCCTCTTCAATCGCTTCGCGACGGGCAACATCTTC        >  SRR4302535.3770561/3‑94 (MQ=60)

                                                                                        ccCCCCCCGGG‑CTTGCCAGGAAACTTAACACCGGTTTGGTCCGTTTGACTATCAGTCCCGCCTCTTCAATCGCTTCGCGACGGGCAACATCTTC        <  SRR4302535.2603880/92‑1 (MQ=60)

                                                                                        C‑CCCCCCGGG‑CTGGCCAGAAAACTTAACACCGGTTTGGTCCTTTTGCCTATCAGTCCCGCCCCTTCAATCGCTTCGCGACGGGCAACATCTTCC       <  SRR4302535.3763438/94‑1 (MQ=60)

                                                                                          CCCCCCGGG‑CTTGCCAGGAAACTTAACACCGGTTTGGTCCGTTTGACTATCAGTCCCGCCTCTTCAATCGCTTCGCGACGGGCAACATCTTCCA      >  SRR4302535.263154/1‑94 (MQ=60)

                                                                                          CCCCCCGGG‑CTTGCCAGGAAACTTAACACCGGTTTGGTCCGTTTGACTATCAGTCCCGCCTCTTCAATCGCTTCGCGACGGGCAACATCTTCCA      >  SRR4302535.4316806/1‑94 (MQ=60)

                                                                                          CCCCCCGGG‑CTTGCCAGGAAACTTAACACCGGTTTGGTCCGTTTGACTATCAGTCCCGCCTCTTCAATCGCTTCGCGACGGGCAACATCTTCCA      >  SRR4302535.3800543/1‑94 (MQ=60)

                                                                                          CCCCCCGGG‑CTTGCCAGGAAACTTAACACCGGTTTGGTCCGTTTGACTATCAGTCCCGCCTCTTCAATCGCTTCGCGACGGGCAACATCTTCCA      >  SRR4302535.1046170/1‑94 (MQ=60)

                                                                                           CCCCCGGG‑CTTGCCAGGAAACTTAACACCGGTTTGGTCCGTTTGACTATCAGTCCCGCCTCTTCAATCGCTTCGCGACGGGCAACATCTTCCAC     >  SRR4302535.1778380/1‑94 (MQ=60)

                                                                                             CCCGGG‑CTTGCCAGGAAACTTAACACCGGTTTGGTCCGTTGGACTATCAGTCCCGCCTCTTCAATCGCTTCGCGACGGGCAACATCTTCCACAC   >  SRR4302535.3408619/1‑94 (MQ=60)

                                                                                              CCGGG‑CTTGCCAGGAAACTTAACACCTGTTTGGGCCGTTTGACTATCAGTCCCGCCTCTTCAATCGCTTCGCGACGGGCAACATCTTCCACACT  <  SRR4302535.2277231/94‑1 (MQ=60)

ATCTTCGTTTTCATCAGCCAGACCGTGAATACCGCTTGCGGTCGTGGCGTCCACTTCGCCCACCATAATTGACGAACGCTCACTGGT‑G‑CCCCCCGGG‑CTTGCCAGGAAACTTAACACCGGTTTGGTCCGTTTGACTATCAGTCCCGCCTCTTCAATCGCTTCGCGACGGGCAACATCTTCCACACT  >  NC_000913/3175432‑3175617

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 2 ≤` `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 31 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`