Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 1696 279.3 15874187 97.8% 15524954 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,284,393 A→G E161E (GAA→GAG)  agaI → putative galactosamine‑6‑phosphate isomerase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,284,3930AG99.6% 708.0 / ‑5.1 228E161E (GAA→GAG) agaIputative galactosamine‑6‑phosphate isomerase
Reads supporting (aligned to +/- strand):  ref base A (0/1);  new base G (120/107);  total (120/108)
Fisher's exact test for biased strand distribution p-value = 4.74e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.10e-01

Only 100 of 277 total aligned reads displayed.

GAACGGGTAACGAACCTGATTGCGCGCAAAGGCGGTCTGGATTTATGCGTTCTCGGATTGGGGAAAAACGG‑TCATCTTGGGCTGAACGAACCGGGAGAAAGCCTGCAACCGGCCTGCCATATCAGTCAACTTGATGCCAGAACACAGCAACATGAGATGTTAAAAACCGCGGGTCGCCC  >  NC_000913/3284304‑3284482
                                                                                          |                                                                                         
gAACGGGTAACGAACCTGATTGCGCGCAAAGGCGGTCTGGATTTATGCGTTCTCGGATTGGGGAAAAACGG‑TCATCTTGGGCTGAACGAGCCg                                                                                        >  2:2458509/1‑93 (MQ=255)
 aaCGGGTAACGAACCTGATTGCGCGCAAAGGCGGTCTGGATTTATGCGTTCTCGGATTGGGGAAAAACGG‑TCATCTTGGGCTGAACGAGCCgg                                                                                       <  1:2040696/93‑1 (MQ=255)
       tAACGAACCTGATTGCGCGCAAAGGCGGTCTGGATTTATGCGTTCTCGGATTGGGGAAAAACGG‑TCATCTTGGGCTGAACGAGCCGGGAGaaa                                                                                 <  1:5121466/93‑1 (MQ=255)
       tAACGAACCTGATTGCGCGCAAAGGCGGTCTGGATTTATGCGTTCTCGGATTGGGGAAAAACGG‑TCATCTTGGGCTGAACGAGCCGGGAGaaa                                                                                 >  1:877736/1‑93 (MQ=255)
       tAACGAACCTGATTGCGCGCAAAGGCGGTCTGGATTTATGCGTTCTCGGATTGGGGAAAAACGG‑TCATCTTGGGCTGAAAGAGCCGGGAGaaa                                                                                 >  1:3966181/1‑93 (MQ=255)
        aaCGAACCTGATTGCGCGCAAAGGCGGTCTGGATTTATGCGTTCTCGGATTGGGGAAAAACGG‑TCATCTTGGGCTGAACGAGCCGGGAGAAAg                                                                                <  1:210107/93‑1 (MQ=255)
        aaCGAACCTGATTGCGCGCAAAGGCGGTCTGGATTTATGCGTTCTCGGATTGGGGAAAAACGG‑TCATCTTGGGCTGAACGAGCCGGGAGAAAg                                                                                <  1:6192238/93‑1 (MQ=255)
          cGAACCTGATTGCGCGCAAAGGCGGTCTGGATTTATGCGTTCTCGGATTGGGGAAAAACGG‑TCATCTTGGGCTGAACGAGCCGGGAGAAAGcc                                                                              <  2:5786255/93‑1 (MQ=255)
          cGAACCTGATTGCGCGCAAAGGCGGTCTGGATTTATGCGTTCTCGGATTGGGGAAAAACGG‑TCATCTTGGGCTGAACGAGCCGGGAGAAAGcc                                                                              <  1:6606299/93‑1 (MQ=255)
            aaCCTGATTGCGCGCAAAGGCGGTCTGGATTTATGCGTTCTCGGATTGGGGAAAAACGG‑TCATCTTGGGCTGAACGAGCCGGGAGAAAGCCTg                                                                            >  1:1082677/1‑93 (MQ=255)
            aaCCTGATTGCGCGCAAAGGCGGTCTGGATTTATGCGTTCTCGGATTGGGGAAAAACGG‑TCATCTTGGGCTGAACGAGCCGGGAGAAAGCCTg                                                                            >  1:7205102/1‑93 (MQ=255)
             aCCTGATTGCGCGCAAAGGCGGTTTGGATTTATGCGTTCTCGGATTGGGGAAAAACGG‑TCATCTTGGGCTGAACGAGCCGGGAGAAAGCCTGc                                                                           <  1:6084694/93‑1 (MQ=255)
               cTGATTGCGCGCAAAGGCGGTCTGGATTTATGCGTTCTCGGATTGGGGAAAAACGG‑TCATCTTGGGCTGAACGAGCCGGGAGAAAGCCTGCaa                                                                         <  2:546238/93‑1 (MQ=255)
                tGATTGCGCGCAAAGGCGGTCTGGATTTATGCGTTCTCGGATTGGGGAAAAACGG‑TCATCTTGGGCTGAACGAGCCGGGAGAAAGCCTGCAAc                                                                        <  1:3186015/93‑1 (MQ=255)
                tGATTGCGCGCAAAGGCGGTCTGGATTTATGCGTTCTCGGATTGGGGAAAAACGG‑TCATCTTGGGCTGAACGAGCCGGGAGAAAGCCTGCAAc                                                                        <  1:5445652/93‑1 (MQ=255)
                 gATTGCGCGCAAAGGCGGTCTGGATTTATGCGTTCTCGGATTGGGGAAAAACGG‑TCATCTTGGGCTGAACGAGCCGGGAGAAAGCCTGCAAcc                                                                       <  1:7728271/93‑1 (MQ=255)
                  aTTGCGCGCAAAGGCGGTCTGGATTTATGCGTTCTCGGATTGGGGAAAAACGG‑TCATCTTGGGCTGAACGAGCCGGGAGAAAGCCTGCAACCg                                                                      <  2:1757555/93‑1 (MQ=255)
                  aTTGCGCGCAAAGGCGGTCTGGATTTATGCGTTCTCGGATTGGGGAAAAACGG‑TCATCTTGGGCTGAACGAGCCGGGAGAAAGCCTGCAACCg                                                                      >  2:4912119/1‑93 (MQ=255)
                  aTTGCGCGCAAAGGCGGTCTGGATTTATGCGTTCTCGGATTGGGGAAAAACGG‑TCATCTTGGGCTGAACGAGCCGGGAGAAAGCCTGCAACCg                                                                      <  1:5788438/93‑1 (MQ=255)
                   ttGCGCGCAAAGGCGGTCTGGATTTATGCGTTCTCGGATTGGGGAAAAACGG‑TCATCTTGGGCTGAACGAGCCGGGAGAAAGCCTGCAACCgg                                                                     >  1:2121668/1‑93 (MQ=255)
                     gcgcgcAAAGGCGGTCTGGATTTATGCGTTCTCGGATTGGGGAAAAACGG‑TCATCTTGGGCTGAACGAGCCGGGAGAAAGCCTGCAACCGGcc                                                                   <  2:1664206/93‑1 (MQ=255)
                     gcgcgcAAAGGCGGTCTGGATTTATGCGTTCTCGGATTGGGGAAAAACGG‑TCATCTTGGGCTGAACGAGCCGGGAGAAAGCCTGCAACCGGcc                                                                   <  2:1501241/93‑1 (MQ=255)
                      cgcgcAAAGGCGGTCTGGATTTATGCGTTCTCGGATTGGGGAAAAACGG‑TCATCTTGGGCTGAACGAGCCGGGAGAAAGCCTGCAACCGGCCt                                                                  >  2:154691/1‑93 (MQ=255)
                         gcAAAGGCGGTCTGGATTTATGCGTTCTCGGATTGGGGAAAAACGG‑TCATCTTGGGCTGAACGAGCCGGGAGAAAGCCTGCAACCGGCCTGcc                                                               >  1:5396687/1‑93 (MQ=255)
                          cAAAGGCGGTCTGTATTTATGCGTTCTCGGATTGGGGAAAAACGG‑TCATCTTGGGCTGAACGAGCCGGGAGAAAGCCTGCAACCGGCCTGCCa                                                              <  1:2895254/93‑1 (MQ=255)
                           aaaGGCGGTCTGGATTTATGCGTTCTCGGATTGGGGAAAAACGG‑TCATCTTGGGCTGAACGAGCCGGGAGAAAGCCTGCAACCGGCCTGCCat                                                             <  2:629431/93‑1 (MQ=255)
                           aaaGGCGGTCTGGATTTATGCGTTCTCGGATTGGGGAAAAACGG‑TCATCTTGGGCTGAACGAGCCGGGAGAAAGCCTGCAACCGGCCTGCCat                                                             >  2:6752530/1‑93 (MQ=255)
                           aaaGGCGGTCTGGATTTATGCGTTCTCGGATGGGGGAAAAACGG‑TCATCTGGGGCTGAACGAGCCGGGAGAAAGCCTGCAACCGGCCTGCCat                                                             <  2:2018030/93‑1 (MQ=255)
                             aGGCGGTCGGGATTTATGCGTTCTCGGATTGGGGAAAACCGG‑TCATCTTGGGCTGAACGAGCCGGGAGAAAGCCTGCAACCGGCCTGCCatat                                                           <  2:3250617/93‑1 (MQ=255)
                              ggCGGTCTGGATTTATGCGTTCTCGGATTGGGGAAAAACGG‑TCATCTTGGGCTGAACGAGCCGGGAGAAAGCCTGCAACCGGCCTGCCATATc                                                          <  2:7619166/93‑1 (MQ=255)
                               gCGGTCTGGATTTATGCGTTCTGGGATTGGGGAAAAACGG‑TCATCTTGGGCTGAACGAGCCGGGAGAAAGCCTGCAACCGGCCTGCCATATCa                                                         <  2:7367189/93‑1 (MQ=255)
                                 ggTCTGGATTTATGCGTTCTCGGATTGGGGAAAAACGG‑TCATCTTGGGCTGAACGAGCCGGGAGAAAGCCTGCAACCGGCCTGCCATATCAGt                                                       <  1:3374037/93‑1 (MQ=255)
                                   tCTGGATTTATGCGTTCTCGGATTGGGGAAAAACGG‑TCATCTTGGGCTGAACGAGCCGGGAGAAAGCCTGCAACCGGCCTGCCATATCAGTCa                                                     <  2:1334756/93‑1 (MQ=255)
                                   tCTGGATTTATGCGTTCTCGGATTGGGGAAAAACGG‑TCATCTTGGGCTGAACGAGCCGGGAGAAAGCCTGCAACCGGCCTGCCATATCAGTCa                                                     >  1:2224444/1‑93 (MQ=255)
                                   tCTGGATTTATGCGTTCTCGGATTGGGGAAAAACGG‑TCATCTTGGGCTGAACGAGCCGGGAGAAAGCCTGCAACCGGCCTGCCATATCAGTCa                                                     >  1:4874348/1‑93 (MQ=255)
                                     tGGATTTATGCGTTCTCGGATTGGGGAAAAACGG‑TCATCTTGGGCTGAACGAGCCGGGAGAAAGCCTGCAACCGGCCTGCCATATCAGTCAAc                                                   <  1:2967051/93‑1 (MQ=255)
                                       gATTTATGCGTTCTCGGATTGGGGAAAAACGG‑TCATCTTGGGCTGAACGAGCCGGGAGAAAGCCTGCAACCGGCCTGCCATATCAGTCAACtt                                                 >  2:6046670/1‑93 (MQ=255)
                                       gATTTATGCGTTCTCGGATTGGGGAAAAACGG‑TCATCTTGGGCTGAACGAGCCGGGAGAAAGCCTGCAACCGGCCTGCCATATCAGTCAACtt                                                 >  1:1569457/1‑93 (MQ=255)
                                         tttATGCGTTCTCGTATGGGGGAAAAACGG‑TCATCTTGGGCTGAACGAGCCGGGAGAAAGCCTGCAACCGGCCTGCCATATCAGTCAACTTGa                                               <  2:6622065/93‑1 (MQ=255)
                                         tttATGCGTTCTCGGATTGGGGAAAAACGG‑TCATCTTGGGCTGAACGAGCCGGGAGAAAGCCTGCAACCGGCCTGCCATATCAGTCAACTTGa                                               >  2:113397/1‑93 (MQ=255)
                                         tttATGCGTTCTCGGATTGGGAAAAACCGG‑TCATCTGGGGCTGAACGAGCCGGGAGAAAGCCTGCAACCGGCCTGCCATATCAGTCAACTTGa                                               <  1:6305112/93‑1 (MQ=255)
                                         tttATGCGTTCTCGGATTGGGAAAAAACGG‑TCATCTTGGGCTGAACGAGCCGGGAGAAAGCCTGCAACCGGCCTGCCATATCAGTCAACTTGa                                               <  1:1370413/93‑1 (MQ=255)
                                         tttATGCGTCCTCGGATTGGGGAAAAACGG‑TCATCTTGGGCTGACCGAGCCGGGAGAAAGCCTGCAACCGGCCTGCCATATCAGTCAACTTGa                                               <  1:1961508/93‑1 (MQ=255)
                                          ttATGCGTTCGCGGATTGGGGAAAAACGG‑TCATCTTGGGCTGAACGAGCCGGGAGAAAGCCTGCAACCGGCCTGCCATATCAGTCAACTTGAt                                              <  1:5044081/93‑1 (MQ=255)
                                           tATGCGTTCTCGGATTGGGGAAAAACGG‑TCATCTTGGGCTGAACGAGCCGGGAGAAAGCCTGCAACCGGCCTGCCATATCAGTCAACTTGATg                                             >  1:4505480/1‑93 (MQ=255)
                                           tATGCGTTCTCGGATTGGGGAAAAACGG‑TCATCTTGGGCTGAACGAGCCGGGAGAAAGCCTGCAACCGGCCTGCCATATCAGTCAACTTGATg                                             <  2:4183090/93‑1 (MQ=255)
                                            ttGCGTTCTCGGATTGGGGAAAAACGG‑TCATCCTGGGCTGACCGAGCCGGGAGAAAGCCTGCAACCGGCCTGCCATATCAGTCAACTTGATGc                                            <  2:4029534/92‑1 (MQ=255)
                                            aTGCGTTCTCGGATTGGGGAAAAACGG‑TCATCTTGGGCTGAACGAGCCGGGAGAAAGCCTGCAACCGGCCTGCCATATCAGTCAACTTGATGc                                            >  1:1657041/1‑93 (MQ=255)
                                            aTGCGTTCTCGGATTGGGGAAAAACGG‑TCATCTTGGGCTGAACGAGCCGGGAGAAAGCCTGCAACCGGCCTGCCATATCAGTCAACTTGATGc                                            <  2:6077355/93‑1 (MQ=255)
                                             tGCGTTCTCGGATTGGGGAAAAACGG‑TCATCTTGGGCTGAACGAGCCGGGAGAAAGCCTGCAACCGGCCTGCCATATCAGTCAACTTGATGcc                                           >  2:7196638/1‑93 (MQ=255)
                                                gTTCTCGGATTGGGGAAAAACGG‑TCATCTTGGGCTGAACGAGCCGGGAGAAAGCCTGCAACCGGCCTGCTATATCAGTCAACTTGATGCCAGa                                        >  2:5354650/1‑93 (MQ=255)
                                                 ttCTCGGATTGGGGAAAAACGG‑TCATCTTGGGCTGAACGAGCCGGGAGAAAGCCTGCAACCGGCCTGCCATATCAGTCAACTTGATGCCAGaa                                       >  1:922413/1‑93 (MQ=255)
                                                 ttCTCGGATTGGGGAAAAACGG‑TCATCTTGGGCTGAACGAGCCGGGAGAAAGCCTGCAACCGGCCTGCCATATCAGTCAACTTGATGCCAGaa                                       >  2:6796579/1‑93 (MQ=255)
                                                  tctcGGATTGGGGAAAAACGG‑TCATCTTGGGCTGAACGAGCCGGGAGAAAGCCTGCAACCGGCCTGCCATATCAGTCAACTTGATGCCAGAac                                      >  2:6389791/1‑93 (MQ=255)
                                                  tctcGGATTGGGAAAAAGCGG‑TCATCTTGGGCTGAACGAGCCGGGAGAAAGCCTGCAACCGGCCTGCCATATCAGTCAACTTGATGCCAGAac                                      <  1:173689/93‑1 (MQ=255)
                                                    tgggATGGGGAAAAAACGG‑TCATCTTGGGCTGAACGAGCCGGGAGAAAGCCTGCAACCGGCCTGCCATATCAGTCAACTTGATGCCAGAacac                                    <  1:6515199/91‑1 (MQ=255)
                                                    tcGGATTGGGGAAAAACGG‑TCATCTGGGGCTGAAGGAGCCGGGAGAAAGCCTGCAACCGGCCTGCCATATCAGTCAACTTGATGCCAGAacac                                    <  2:2166745/93‑1 (MQ=255)
                                                    cgggATTGGGAAAAAACGG‑TCATCTGGGGCTGAACGAGCCGGGAGAAAGCCTGCACCCGGCCTGCCATATCAGTCAACTTGATGCCAGAacac                                    <  1:4573184/91‑1 (MQ=255)
                                                     cGGATTGGGGAAAAACGG‑TCATCTTGGGCTGAACGAGCCGGGAGAAAGCCTGCAACCGGCCTGCCATATCAGTCAACTTGATGCCAGAAcaca                                   >  2:5703228/1‑93 (MQ=255)
                                                     cGGATTGGGAAAAAACGG‑TCATCTGGGGCTGAACGAGCCGGGAGAAAGCCTGCAACCGGCCTGCCATATCAGTCAACTTGATGCCAGAAcaca                                   <  2:2596807/93‑1 (MQ=255)
                                                     cGGACTGGGGAAAACCGT‑TCACCTTGCGCGAATCGAGCCGGGAGAAAGCCTGCAACCGGCCTGCCATATCAGTCAACTTGATGCCAGAAcaca                                   <  1:6935790/93‑1 (MQ=255)
                                                      ggATTGGGGAAAAACGG‑TCATCTTGGGCTGAACGAGCCGGGAGAAAGCCTGCAACCGGCCTGCCATATCAGTCAACTTGATGCCAGAACACAg                                  >  2:2837057/1‑93 (MQ=255)
                                                       gATTGGGGAAAAACGG‑TCATCTTGGGCTGAACGAGCCGGGAGAAAGCCTGCAACCGGCCTGCCATATCAGTCAACTTGATGCCAGAACACAGc                                 >  1:6044350/1‑93 (MQ=255)
                                                       gATTGGGGAAAAACGG‑TCATCTTGGGCTGAACGAGCCGGGAGAAAGCCTGCAACCGGCCTGCCATATCAGTCAACTTGATGCCAGAACACAGc                                 >  2:5652968/1‑93 (MQ=255)
                                                        atgggggAAAAACGT‑TCATCTGGGGCTGAACGAGCCGGGAGAAAGCCTGCAACCGGCCTGCCATATCAGTCAACTTGATGCCAGAACACAGCa                                <  2:2928193/90‑1 (MQ=255)
                                                         ttGGGGAAAAACGG‑TCATCTTGGGCTGAACGAGCCGGGAGAAAGCCTGCAACCGGCCTGCCATATCAGTCAACTTGATGCCAGAACACAGCaa                               >  2:7018003/1‑93 (MQ=255)
                                                         ttGGGGAAAAACGG‑TCATCTTGGGCTGAACGAGCCGGGAGAAAGCCTGCAACCGGCCTGCCATATCAGTCAACTTGATGCCAGAACACAGCaa                               >  1:4085020/1‑93 (MQ=255)
                                                         ttGGGGAAAAACGG‑TCATCTTGGGCTGAACGAGCCGGGAGAAAGCCTGCAACCGGCCTGCCATATCAGTCAACTTGATGCCAGAACACAGCaa                               >  2:1890695/1‑93 (MQ=255)
                                                         tgggggAAAAACGG‑TCATCTTGGGCTGAACGAGCCGGGAGAAAGCCTGCAACCGGCCTGCCATATCAGTCAACTTGATGCCAGAACACAGCaa                               <  2:3936146/91‑1 (MQ=255)
                                                            gggAAAAACGG‑TCATCTTGGGCTGAACGAGCCGGGAGAAAGCCTGCAACCGGCCTGCCATATCAGTCAACTTGATGCCAGAACACAGACACAt                            >  1:6287899/1‑93 (MQ=255)
                                                              gAAAACCGG‑TCATCTTGGGCGGAACGAGCCGGGAGAAAGCCTGCAACCGGCCTGCCATATCAGTCAACTTGATGCCAGAACACAGCAACATga                          <  1:5752902/93‑1 (MQ=255)
                                                               aaaaaCGG‑TCATCTTGGGCTGAACGAGCCGGGAGAAAGCCTGCAACCGGCCTGCCATATCAGTCAACTTGATGCCAAAACACAGCAACAttaa                         >  2:6578627/1‑90 (MQ=255)
                                                                aaaaCGGTTCATCTGGGGCTGACGGAGCCGGGAGAAAGCCTGCAACCGGCCTGCCATATCAGTCAACTTGATGCCAGAACACAGCAACATgag                         <  2:5901834/93‑1 (MQ=255)
                                                                 aagcGG‑TCGTCTGGGGCTGAACGAGCCGGGAGAAAGCCTGCAACCGGCCTGCCATATCAGTCAACTTGATGCCAGAACACAGCAACATGAGAt                       <  1:3549928/90‑1 (MQ=255)
                                                                   aCGG‑TCATCTGGGGCTGAACGAGCCGGGAGAAAGCCTGCAACGGGCCTGCCATATCAGTCAACTTGATGCCAGAACACAGCAACATGAGATGt                     <  2:5011224/93‑1 (MQ=255)
                                                                     gg‑TCATCTTGGGCTGAGGGAGCCGGGAGAAAGCCTGCAACCGGCCTGCCATATCAGTCAACTTGATGCCAGAACACAGCAACATGAGATGTTa                   <  2:4655829/93‑1 (MQ=255)
                                                                      g‑TCATCTTGGGCTGAACGAGCCGGGAGAAAGCCTGCAACCGGCCTGCCATATCAGTCAACTTGATGCCAGAACACAGCAACATGAGATGTTaa                  >  2:2627820/1‑93 (MQ=255)
                                                                      g‑TCATCTGGGGCGGAACGAGCCGGGAGAAAGCCTGCAACCGGCCTGCCATATCAGTCAACTTGATGCCAGAACACAGCAACATGAGATGTTaa                  <  1:2414481/93‑1 (MQ=255)
                                                                       gtaaTCTGGGGCTGAACGAGCCGGGAGAAAGCCTGCAACCGGCCTGCCATATCAGTCAACTTGATGCCAGAACACAGCAACATGAGATGTTaa                  <  2:827621/90‑1 (MQ=255)
                                                                        tCATCTTGGGCTGAACGAGCCGGGAGAAAGCCTGCAACCGGCCTGCCATATCAGTCAACTTGATGCCAGAACACAGCAACATGAGATGTTaaa                 >  1:3793799/1‑93 (MQ=255)
                                                                         cATTTTGGGCTGAACGAGCCGGGAGAAAGCCTGCACCCGGCCTGCCATATCAGTCAACTTGATGCCAGAACACAGCAACATGAGATGTTaaaa                <  2:1079490/93‑1 (MQ=255)
                                                                         atccTTGGGCGGAGCGAGCCGGGAGAAAGCCTGCAACCGGCCTGCCATATCAGTCAACTTGATGCCAGAACACAGCAACATGAGATGTTaaaa                <  1:2914085/90‑1 (MQ=255)
                                                                          aTCTTGGGCTGAACGAGCCGGGAGAAAGCCTGCAACCGGCCTGCCATATCAGTCAACTTGATGCCAGAACACAGCAACATGAGATGTTaaaaa               >  1:2716698/1‑93 (MQ=255)
                                                                           tCTTGGGCTGAACGAGCCGGGAGAAAGCCTGCAACCGGCCTGCCATATCAGTCAACTTGATGCCAGCACACAGCAACATGAGATGTTAAAAAc              >  1:6903352/1‑93 (MQ=255)
                                                                           tCTTGGGCTGAACGAGCCGGGAGAAAGCCTGCAACCGGCCTGCCATATCAGTCAACTTGATGCCAGAACACAGCAACATGAGATGTTAAAAAc              >  2:5134450/1‑93 (MQ=255)
                                                                           tCTTGGGCTGAACGAGCCGGGAGAAAGCCTGCAACCGGCCTGCCATATCAGTCAACTTGATGCCAGAACACAGCAACATGAGATGTTAAAAAc              >  1:5569469/1‑93 (MQ=255)
                                                                           tCTTGGGCTGAACGAGCCGGGAGAAAGCCTGCAACCGGCCTGCCATATCAGTCAACTTGATGCCAGAACACAGCAACATGAGATGTTAAAAAc              >  1:5868818/1‑93 (MQ=255)
                                                                           tCTGGGGCTGAGCGAGCCGGGAGAAAGCCTGCAACCGGCCTGCCATATCAGTCAACTTGATGCCAGAACACAGCAACATGAGATGTTAAAAAc              <  2:3156335/93‑1 (MQ=255)
                                                                            ttggggCTGAGCGAGCCGGGAGAAAGCCTGCAACCGGCCTGCCATATCAGTCAACTTGATGCCAGAACACAGCAACATGAGATGTTAAAAAcc             <  1:7279440/90‑1 (MQ=255)
                                                                             ttGGGCTGAACGAGCCGGGAGAAAGCCTGCAACCGGCCTGCCATATCAGTCAACTTGATGCCAGAACACAGCAACATGAGATGTTAAAAACcg            >  2:1273068/1‑93 (MQ=255)
                                                                              tGGGCTGACCGAGCCGGGAAAAAGCCTGCAACCGGCCTGCCATATCAGTCAACTTGATGCCAGAACACAGCAACATGAGATGTTAAAAACcgc           <  1:7538273/93‑1 (MQ=255)
                                                                              ggggCTAAACGAGCCGGGAGAAAGCCTGCAACCGGCCTGCCATATCAGTCAACTTGATGCCAGAACACAGCAACATGAGATGTTAAAAACcgc           <  1:7720173/92‑1 (MQ=255)
                                                                                  cTGAACGAGCCGGGAGAAAGCCTGCAACCGGCCTGCCATATCAGTCAACTTGATGCCAGAACACAGCAACATGAGATGTTAAAAACCGCGGGt       >  2:3361623/1‑93 (MQ=255)
                                                                                   tGAACGAGCGGGGAGAAAGCCTGCAACCGGCCTGCCATATCAGTCAACTTGATGCCAGAACACAGCAACATGAGATGTTAAAAACCGCGGGTc      <  1:7077338/93‑1 (MQ=255)
                                                                                   tGAACGAGCCGGGAGAAAGCCTGCAACCGGCCTGCCATATCAGTCAACTTGATGCCAGAACACAGCAACATGAGATGTTAAAAACCGCGGGTc      >  2:2385809/1‑93 (MQ=255)
                                                                                    gaacgagcCGGGAGAAAGGCTGCAACCGGCCTGCCATATCAGTCAACTTGATGCCAGAACACAGCAACATGAGATGTTAAAAACCGCGGGTcc     >  1:3493191/1‑92 (MQ=255)
                                                                                    gaacgagcCGGGAGAAAGCCTGCAACCGGCCTGCCATATCAGTCAACTTGATGCCAGAACACAGCAACATGAGATGTTAAAAACCGCGGGTCg     >  1:1197207/1‑93 (MQ=255)
                                                                                    gaacgagcCGGGAGAAAGCCTGCAACCGGCCTGCCATATCAGTCAACTTGATGCCAGAACACAGCAACATGAGATGTTAAAAACCGCGGGTCg     >  2:6426321/1‑93 (MQ=255)
                                                                                    gaacgagcCGGGAGAAAGCCTGCAACCGGCCTGCCATATCAGTCAACTTGATGCCAGAACACAGCAACATGAGATGTTAAAAACCGCGGGTCg     >  1:7832257/1‑93 (MQ=255)
                                                                                      acgagcGGGGAGAAAGCCTGCACCCGGCCTGCCATATCAGTCAACTTGATGCCAGAACACAGCAACATGAGATGTTAAAAACCGCGGGTCAcc   <  2:4322727/93‑1 (MQ=255)
                                                                                          |                                                                                         
GAACGGGTAACGAACCTGATTGCGCGCAAAGGCGGTCTGGATTTATGCGTTCTCGGATTGGGGAAAAACGG‑TCATCTTGGGCTGAACGAACCGGGAGAAAGCCTGCAACCGGCCTGCCATATCAGTCAACTTGATGCCAGAACACAGCAACATGAGATGTTAAAAACCGCGGGTCGCCC  >  NC_000913/3284304‑3284482

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GTGCGAACGGGTAACGAACCTGATTGCGCGCAAAGGCGGTCTGGATTTATGCGTTCTCGGATTGGGGAAAAACGGTCATCTTGGGCTGAACGAACCGGGAGAAAGCCTGCAACCGGCCTGCCATATCAGTCAACTTGATGCCAGAACACAGCAACATGAGATGTTAAAAACCGCGGGTCGCCCCGT  >  NC_000913/3284300‑3284485
                                                                                             |                                                                                            
GTGCGAACGGGTAACGAACCTGATTGCGCGCAAAGGCGGTCTGGATTTATGCGTTCTCGGATTGGGGAAAAACGGTCATCTTGGGCTGAACGAG                                                                                              <  SRR4302543.6616100/94‑1 (MQ=60)
GTGCGAACGGGTAACGAACCTGATTGCGCGCAAAGGCGGTCTGGATTTATGCGTTCTCGGATTGGGGAAAAACGGTCATCTTGGGCTGAACGAG                                                                                              <  SRR4302543.6616099/94‑1 (MQ=60)
GTGCGAACGGGTAACGAACCTGATTGCGCGCAAAGGCGGTCTGGATTTATGCGTTCTCGGATTGGGGAAAAACGGTCATCTTGGGCTGAACGAG                                                                                              <  SRR4302543.536628/94‑1 (MQ=60)
 GGCGAACGGGTAACGAACCTGATTGCGCGCAAAGGCGGTCTGGATTTATGCGTTCTCGGATTGGGGAAAAACGGTCATCTTGGGCTGAACGAGC                                                                                             <  SRR4302543.6768501/94‑1 (MQ=60)
   CGAACGGGTAACGAACCTGATTGCGCGCAAAGGCGGTCTGGATTTATGCGTTCTCGGATTGGGGAAAAACGGTCATCTTGGGCTGAACGAGCCG                                                                                           >  SRR4302543.5078511/1‑94 (MQ=60)
    GAACGGGTAACGAACCTGATTGCGCGCAAAGGCGGTCTGGATTTATGCGTTCTCGGATTGGGGAAAAACGGTCATCTTGGGCTGAACGAGCCGG                                                                                          >  SRR4302543.19640/1‑94 (MQ=60)
     AACGGGTAACGAACCTGATTGCGCGCAAAGGCGGTCTGGATTTATGCGTTCTCGGATTGGGGAAAAACGGTCATCTTGGGCTGAACGAGCCGGG                                                                                         >  SRR4302543.3369803/1‑94 (MQ=60)
     AACGGGTAACGAACCTGATTGCGCGCAAAGGCGGTCTGGATTTATGCGTTCTCGGATTGGGGAAAAACGGTCATCTTGGGCTGAACGAGCCGGG                                                                                         <  SRR4302543.2059002/94‑1 (MQ=60)
     AACGGGTAACGAACCTGATTGCGCGCAAAGGCGGTCTGGATTTATGCGTTCTCGGATTGGGGAAAAACGGTCATCTTGGGCTGAACGAGCCGGG                                                                                         >  SRR4302543.6919449/1‑94 (MQ=60)
     AACGGGTAACGAACCTGATTGCGCGCAAAGGCGGTCTGGATTTATGCGTTCTCGGATTGGGGAAAAACGGTCATCTTGGGCTGAACGAGCCGGG                                                                                         <  SRR4302543.2059001/94‑1 (MQ=60)
      ACGGGTAACGAGCCTGATTGCGCGCAAAGGCGGTCTGGATTTATGCGTTCTCGGATTGGGGAAAAACGGTCATCTTGGGCTGAACGAGCCGGGA                                                                                        <  SRR4302543.2143955/94‑1 (MQ=60)
       CGGGTAACGAACCTGATTGCGCGCAAAGGCGGTCTGGATTTATGCGTTCTCGGATTGGGGAAAAACGGTCATCTTGGGCTGAACGAGCCGGGAG                                                                                       >  SRR4302543.3966227/1‑94 (MQ=60)
          GTAACGAACCTGATTGCGCGCAAAGGCGGTCTGGATTTATGCGTTCTCGGATTGGGGAAAAACGGTCATCTTGGGCTGAACGAGCCGGGAGAAA                                                                                    >  SRR4302543.885533/1‑94 (MQ=60)
          GTAACGAACCTGATTGCGCGCAAAGGCGGTCTGGATTTATGCGTTCTCGGATTGGGGAAAAACGGTCATCTTGGGCTGAACGAGCCGGGAGAAA                                                                                    <  SRR4302543.7277914/94‑1 (MQ=60)
          GTAACGAACCTGATTGCGCGCAAAGGCGGTCTGGATTTATGCGTTCTCGGATTGGGGAAAAACGGTCATCTTGGGCTGAAAGAGCCGGGAGAAA                                                                                    >  SRR4302543.4001318/1‑94 (MQ=60)
           TAACGAACCTGATTGCGCGCAAAGGCGGTCTGGATTTATGCGTTCTCGGATTGGGGAAAAACGGTCATCTTGGGCTGAACGAGCCGGGAGAAAG                                                                                   <  SRR4302543.933702/94‑1 (MQ=60)
           TAACGAACCTGATTGCGCGCAAAGGCGGTCTGGATTTATGCGTTCTCGGATTGGGGAAAAACGGTCATCTTGGGCTGAACGAGCCGGGAGAAAG                                                                                   <  SRR4302543.5167773/94‑1 (MQ=60)
           TAACGAACCTGATTGCGCGCAAAGGCGGTCTGGATTTATGCGTTCTCGGATTGGGGAAAAACGGTCATCTTGGGCTGAACGAGCCGGGAGAAAG                                                                                   >  SRR4302543.7898244/1‑94 (MQ=60)
            AACGAACCTGATTGCGCGCAAAGGCGGTCTGGATTTATGCGTTCTCGGATTGGGGAAAAACGGTCATCTTGGGCTGAACGAGCCGGGAGAAAGC                                                                                  <  SRR4302543.6250743/94‑1 (MQ=60)
            AACGAACCTGATTGCGCGCAAAGGCGGTCTGGATTTATGCGTTCTCGGATTGGGGAAAAACGGTCATCTTGGGCTGAACGAGCCGGGAGAAAGC                                                                                  <  SRR4302543.211898/94‑1 (MQ=60)
            AACGAACCTGATTGCGCGCAAAGGCGGTCTGGATTTATGCGTTCTCGGATTGGGGAAAAACGGTCATCTTGGGCTGAACGAGCCGGGAGAAAGC                                                                                  >  SRR4302543.2767585/1‑94 (MQ=60)
             ACGAACCTGATTGCGCGCAAAGGCGGTCTGGATTTATGCGTTCTCGGATTGGGGAAAAACGGTCATCTTGGGCTGAACGAGCCGGGAGAAAGCC                                                                                 >  SRR4302543.2704412/1‑94 (MQ=60)
              CGAACCTGATTGCGCGCAAAGGCGGTCTGGATTTATGCGTTCTCGGATTGGGGAAAAACGGTCATCTTGGGCTGAACGAGCCGGGAGAAAGCCT                                                                                >  SRR4302543.1915839/1‑94 (MQ=60)
              CGAACCTGATTGCGCGCAAAGGCGGTCTGGATTTATGCGTTCTCGGATTGGGGAAAAACGGTCATCTTGGGCTGAACGAGCCGGGAGAAAGCCT                                                                                <  SRR4302543.6668564/94‑1 (MQ=60)
               GAACCTGATTGCGCGCAAAGGCGGTCTGGATTTATGCGTTCTCGGATTGGGGAAAAACGGTCATCTTGGGCTGAACGAGCCGGGAGAAAGCCTG                                                                               <  SRR4302543.5295498/94‑1 (MQ=60)
               GAACCTGATTGCGCGCAAAGGCGGTCTGGATTTATGCGTTCTCGGATTGGGGAAAAACGGTCATCTTGGGCTGAACGAGCCGGGAGAAAGCCTG                                                                               >  SRR4302543.7274955/1‑94 (MQ=60)
               GAACCTGATTGCGCGCAAAGGCGGTCTGGATTTATGCGTTCTCGGATTGGGGAAAAACGGTCATCTTGGGCTGAACGAGCCGGGAGAAAGCCTG                                                                               >  SRR4302543.1092406/1‑94 (MQ=60)
                 ACCTGATTGCGCGCAAAGGCGGTTTGGATTTATGCGTTCTCGGATTGGGGAAAAACGGTCATCTTGGGCTGAACGAGCCGGGAGAAAGCCTGCA                                                                             <  SRR4302543.6142359/94‑1 (MQ=60)
                 ACCTGATTGCGAGCGAAGGCGGTCTGGATAAATGCAATAGCGAATTGGGGAAAAACGGTCATATTGGGCTGAACGAGCCGGGAGAAAGCCTGCA                                                                             <  SRR4302543.7087736/94‑1 (MQ=36)
                  CCTGATTGCGCGCAAAGGCGGTCTGGATTTATGCGTTCTCGGATTGGGGAAAAACGGTCATCTTGGGCTGAACGAGCCGGGAGAAAGCCTGCAA                                                                            >  SRR4302543.2132353/1‑94 (MQ=60)
                   CTGATTGCGCGCAAAGGCGGTCTGGATTTATGCGTTCTCGGATTGGGGAAAAACGGTCATCTTGGGCTGAACGAGCCGGGAGAAAGCCTGCAAC                                                                           <  SRR4302543.3755270/94‑1 (MQ=60)
                    TGATTGCGCGCAAAGGCGGTCTGGATTTATGCGTTCTCGGATTGGGGAAAAACGGTCATCTTGGGCTGAACGAGCCGGGAGAAAGCCTGCAACC                                                                          <  SRR4302543.3214749/94‑1 (MQ=60)
                    TGATTGCGCGCAAAGGCGGTCTGGATTTATGCGTTCTCGGATTGGGGAAAAACGGTCATCTTGGGCTGAACGAGCCGGGAGAAAGCCTGCAACC                                                                          <  SRR4302543.5809703/94‑1 (MQ=60)
                    TGATTGCGCGCAAAGGCGGTCTGGATTTATGCGTTCTCGGATTGGGGAAAAACGGTCATCTTGGGCTGAACGAGCCGGGAGAAAGCCTGCAACC                                                                          <  SRR4302543.5495070/94‑1 (MQ=60)
                    TGATTGCGCGCAAAGGCGGTCTGGATTTATGCGTTCTCGGATTGGGGAAAAACGGTCATCTTGGGCTGAACGAGCCGGGAGAAAGCCTGCAACC                                                                          <  SRR4302543.7045969/94‑1 (MQ=60)
                     GATTGCGCGCAAAGGCGGTCTGGATTTATGCGTTCTCGGATTGGGGAAAAACGGTCATCTTGGGCTGAGCGAGCCGGGAGAAAGCCTGCAACCG                                                                         <  SRR4302543.7185818/94‑1 (MQ=60)
                     GATTGCGCGCAAAGGCGGTCTGGATTTATGCGTTCTCGGATTGGGGAAAAACGGTCATCTTGGGCTGAACGAGCCGGGAGAAAGCCTGCAACCG                                                                         <  SRR4302543.5871284/94‑1 (MQ=60)
                     GATTGCGCGCAAAGGCGGTCTGGATTTATGCGTTCTCGGATTGGGGAAAAACGGTCATCTTGGGCTGAACGAGCCGGGAGAAAGCCTGCAACCG                                                                         <  SRR4302543.567101/94‑1 (MQ=60)
                     GATTGCGCGCAAAGGCGGTCTGGATTTATGCGTTCTCGGATTGGGGAAAAACGGTCATCTTGGGCTGAACGAGCCGGGAGAAAGCCTGCAACCG                                                                         <  SRR4302543.7658751/94‑1 (MQ=60)
                     GATTGCGCGCAAAGGCGGTCTGGATTTATGCGTTCTCGGATTGGGGAAAAACGGTCATCTTGGGCTGAACGAGCCGGGAGAAAGCCTGCAACCG                                                                         <  SRR4302543.7801317/94‑1 (MQ=60)
                     GATTGCGCGCAAAGGCGGTCTGGATTTATGCGTTCTCGGATTGGGGAAAAACGGTCATCTTGGGCTGAACGAGCCGGGAGAAAGCCTGCAACCG                                                                         <  SRR4302543.5457679/94‑1 (MQ=60)
                      ATTGCGCGCAAAGGCGGTCTGGATTTATGCGTTCTCGGATTGGGGAAAAACGGTCATCTTGGGCTGAACGAGCCGGGAGAAAGCCTGCAACCGG                                                                        >  SRR4302543.2140666/1‑94 (MQ=60)
                      ATTGCGCGCAAAGGCGGTCTGGATTTATGCGTTCTCGGATTGGGGAAAAACGGTCATCTTGGGCTGAACGAGCCGGGAGAAAGCCTGCAACCGG                                                                        <  SRR4302543.5841106/94‑1 (MQ=60)
                         GCGCGCAAAGGCGGTGTGGATTTATGCGTTCTGGTATTGGGGAAAAACGGTCATCTTGGGCTGAACGAGCCGGGAGAAAGCCTGCAACCGGCCT                                                                     <  SRR4302543.5545220/94‑1 (MQ=60)
                         GCGCGCAAAGGCGGTCTGGATTTATGCGTTCTCGGATTGGGGAAAAACGGTCATCTTGGGCTGAACGAGCCGGGAGAAAGCCTGCAACCGGCCT                                                                     <  SRR4302543.6870823/94‑1 (MQ=60)
                         GCGCGCAAAGGCGGTCTGGATTTATGCGTTCTCGGATTGGGGAAAAACGGTCATCTTGGGCTGAACGAGCCGGGAGAAAGCCTGCAACCGGCCT                                                                     <  SRR4302543.1590269/94‑1 (MQ=60)
                         GCGCGCAAAGGCGGTCTGGATTTATGCGTTCTCGGATTGGGGAAAAACGGTCATCTTGGGCTGAACGAACCGGGAGAAAGCCTGCAACCGGCCT                                                                     <  SRR4302543.3397238/94‑1 (MQ=60)
                           GCGCAAAGGCGGTCTGGATTTATGCGTTCTCGGATTGGGGAAAAACGGTCATCTTGGGCTGAACGAGCCGGGAGAAAGCCTGCAACCGGCCTGC                                                                   <  SRR4302543.2114761/94‑1 (MQ=60)
                           GCGCAAAGGCGGTCTGGATTTATGCGTTCTCGGATTGGGGAAAAACGGTCATCTGGGGCTGAACGAGCCGGGAGAAAGCCTGCAACCGGCCTGC                                                                   <  SRR4302543.4019405/94‑1 (MQ=60)
                            CGCAAAGGCGGTCTGGATTTATGCGTTCTCGGATTGGGGAAAAACGGTCATCTTGGGCTGAACGAGCCGGGAGAAAGCCTGCAACCGGCCTGCC                                                                  >  SRR4302543.3434333/1‑94 (MQ=60)
                            CGCAAAGGCGGTCTGGATTTATGCGTTCTCGGATTGGGGAAAAACGGTCATCTTGGGCTGAACGAGCCGGGAGAAAGCCTGCAACCGGCCTGCC                                                                  >  SRR4302543.5445761/1‑94 (MQ=60)
                             GCAAAGGCGGTCTGGATTTATGCGTTCTCGGATTGGGGAAAAACGGTCATCTTGGGCTGAACGAGCCGGGAGAAAGCCTGCAACCGGCCTGCCA                                                                 >  SRR4302543.3910685/1‑94 (MQ=60)
                             GCAAAGGCGGTCTGGATTTATGCGTTCTCGGATTGGGGAAAAACGGTCATCTTGGGCTGAACGAGCCGGGAGAAAGCCTGCAACCGGCCTGCCA                                                                 >  SRR4302543.6989922/1‑94 (MQ=60)
                             GCAAAGGCGGTCTGGATTTATGCGTTCTCGGATTGGGGAAAAACGGTCATCTTGGGCTGAACGAGCCGGGAGAAAGCCTGCAACCGGCCTGCCA                                                                 >  SRR4302543.7034903/1‑94 (MQ=60)
                              CAAAGGCGGTCTGTATTTATGCGTTCTCGGATTGGGGAAAAACGGTCATCTTGGGCTGAACGAGCCGGGAGAAAGCCTGCAACCGGCCTGCCAT                                                                <  SRR4302543.2921578/94‑1 (MQ=60)
                              CAAAGGCGGTCTGGATTTATGCGTTCTCGGATTGGGGAAAAACGGTCATCTTGGGCTGAACGAGCCGGGAGAAAGCCTGCAACCGGCCTGCCAT                                                                <  SRR4302543.4792977/94‑1 (MQ=60)
                              CAAAGGCGGTCTGGATTTATGCGTTCTCGGATTGGGGAAAAACGGTCATCTTGGGCTGAACGAGCCGGGAGAAAGCCTGCAACCGGCCTGCCAT                                                                >  SRR4302543.1001338/1‑94 (MQ=60)
                               AAAGGCGGTCTGGATTTATGCGTTCTCGGATTGGGGAAAAACGGTCATCTTGGGCTGAACGAGCCGGGAGAAAGCCTGCAACCGGCCTGCCATA                                                               >  SRR4302543.158959/1‑94 (MQ=60)
                               AAAGGCGGTCTGGATTTATGCGTTCTCGGATTGGGGAAAAACGGTCATCTTGGGCTGAACGAGCCGGGAGAAAGCCTGCAACCGGCCTGCCATA                                                               >  SRR4302543.802878/1‑94 (MQ=60)
                                AAGGCGGTCTGGATTTATGCGTTCTCGGATTGGGGAAAAACGGTCATCTTGGGCTGAACGAGCCGGGAGAAAGCCTGCAACCGGCCTGCCATAT                                                              >  SRR4302543.6207618/1‑94 (MQ=60)
                                AAGGCGGTCTGGATTTATGCGTTCTCGGATTGGGGAAAAACGGTCATCTTGGGCTGAACGAGCCGGGAGAAAGCCTGCAACCGGCCTGCCATAT                                                              <  SRR4302543.5133545/94‑1 (MQ=60)
                                  GGCGGTCTGGATTTATGCGTTCTCGGATTGGGGAAAAACGTTCATCTTGGGCTGAACGAGCCGGGAGAAAGCCTGCAACCGGCCTGCCATATCA                                                            <  SRR4302543.2289063/94‑1 (MQ=60)
                                   GCGGTCTGGATTTATGCGTTCTCGGATTGGGGAAAAACGGTCATCTTGGGCTGAACGAGCCGGGAGAAAGCCTGCAACCGGCCTGCCATATCAG                                                           >  SRR4302543.3139415/1‑94 (MQ=60)
                                   GCGGTCTGGAGTTATGCGTTCTCGGATTGGGGAAAAACGGTCATCTTGGGCTGAACGAGCCGGGAGAAAGCCTGCAACCGGCCTGCCATATCAG                                                           <  SRR4302543.7555080/94‑1 (MQ=60)
                                    CGGTCTGGATTTATGCGTTCTCGGATTGGGGAAAAACGGTCATCTTGGGCTGAACGAGCCGGGAGAAAGCCTGCAACCGGCCTGCCATATCAGT                                                          <  SRR4302543.5452238/94‑1 (MQ=60)
                                     GGTGTGGTTTTATGGGTTCTCGGATTGGGGAAAACGGGTCATCTGGGGCTGAACGAGCCGGGAGAAAGCCTGCAACCGGCCTGCCATATCAGTC                                                         <  SRR4302543.6308785/94‑1 (MQ=60)
                                     GGTCTGGATTTATGCGTTCTCGGATTGGGGAAAAACGGTCATCTTGGGCTGAACGAGCCGGGAGAAAGCCTGCAACCGGCCTGCCATATCAGTC                                                         <  SRR4302543.3404332/94‑1 (MQ=60)
                                     GGTCTGGATTTATGCGTTCTCGGATTGGGGAAAAACGGTCATCTTGGGCTGAACGAGCCGGGAGAAAGCCTGCAACCGGCCTGCCATATCAGTC                                                         <  SRR4302543.1310563/94‑1 (MQ=60)
                                     GGTCTGGATTTATGCGTTCTCGGATTGGGGAAAAACGGTCATCTTGGGCTGAACGAGCCGGGAGAAAGCCTGCAACCGGCCTGCCATATCAGTC                                                         >  SRR4302543.1701477/1‑94 (MQ=60)
                                      GTCTGGATTTATGCGTTCTCGGATTGGGGAAAAACGGTCATCTTGGGCTGAACGAGCCGGGAGAAAGCCTGCAACCGGCCTGCCATATCAGTCA                                                        >  SRR4302543.4918778/1‑94 (MQ=60)
                                      GTCTGGATTTATGCGTTCTCGGATTGGGGAAAAACGGTCATCTTGGGCTGAACGAGCCGGGAGAAAGCCTGCAACCGGCCTGCCATATCAGTCA                                                        >  SRR4302543.2244523/1‑94 (MQ=60)
                                       TCTGGATTTATGCGTTCTCGGATTGGGGAAAAACGGTCATCTTGGGCTGAACGAGCCGGGAGAAAGCCTGCAACCGGCCTGCCATATCAGTCAA                                                       <  SRR4302543.7847615/94‑1 (MQ=60)
                                        CTGGATTTATGCGTTCTCGGATTGGGGAAAAACGGTCATCTTGGGCTGAACGAGCCGGGAGAAAGCCTGCAACCGGCCTGCCATATCAGTCAAC                                                      >  SRR4302543.5931517/1‑94 (MQ=60)
                                         TGGATTTATGCGTTCTCGGATTGGGGAAAAACGGTCATCTTGGGCTGAACGAGCCGGGAGAAAGCCTGCAACCGGCCTGCCATATCAGTCAACT                                                     <  SRR4302543.4889885/94‑1 (MQ=60)
                                         TGGATTTATGCGTTCTCGGATTGGGGAAAAACGGTCATCTTGGGCTGAACGAGCCGGGAGAAAGCCTGCAACCGGCCTGCCATATCAGTCAACT                                                     <  SRR4302543.2993994/94‑1 (MQ=60)
                                          GGATTTATGCGTTCTCGGATTGGGGAAAAACGGTCATCTTGGGCTGAACGAGCCGGGAGAAAGCCTGCAACCGGCCTGCCATATCAGTCAACTT                                                    >  SRR4302543.6623605/1‑94 (MQ=60)
                                          GGATTTATGCGTTCTCGGATTGGGGAAAAACGGTCATCTTGGGCTGAACGAGCCGGGAGAAAGCCTGCAACCGGCCTGCCATATCAGTCAACTT                                                    >  SRR4302543.1583470/1‑94 (MQ=60)
                                          GGATTTATGCGTTCTCGGATTGGGGAAAAACGGTCATCTTGGGCTGAACGAGCCGGGAGAAAGCCTGCAACCGGCCTGCCATATCAGTCAACTT                                                    >  SRR4302543.3199198/1‑94 (MQ=60)
                                           GATTTATGCGTTCTCGGATTGGGGAAAAACGGTCATCTTGGGCTGAACGAGCCGGGAGAAAGCCTGCAACCGGCCTGCCATATCAGTCAACTTG                                                   >  SRR4302543.2759886/1‑94 (MQ=60)
                                            attttggggtcctcggtttggaaaatatcgtccctctgGGGCTGAAGGAGCCGGGAGAAAGCCTGAAACCGGCCTGCCATATCAGTCAACTTGA                                                  <  SRR4302543.3046761/56‑1 (MQ=59)
                                            ATTTATGCGTTCTCGGATTGGGGAAAAACGGTCATCTTGGGCTGAACGAGCCGGGAGAAAGCCTGCAACCGGCCTGCCATATCAGTCAACTTGA                                                  >  SRR4302543.901470/1‑94 (MQ=60)
                                             TTTATGCGTTCTCGGATTGGGAAAAACCGGTCATCTGGGGCTGAACGAGCCGGGAGAAAGCCTGCAACCGGCCTGCCATATCAGTCAACTTGAT                                                 <  SRR4302543.6364423/94‑1 (MQ=60)
                                             TTTATGCGTTCTCGGATTGGGAAAAAACGGTCATCTTGGGCTGAACGAGCCGGGAGAAAGCCTGCAACCGGCCTGCCATATCAGTCAACTTGAT                                                 <  SRR4302543.1382717/94‑1 (MQ=60)
                                             TTTATGCGTCCTCGGATTGGGGAAAAACGGTCATCTTGGGCTGACCGAGCCGGGAGAAAGCCTGCAACCGGCCTGCCATATCAGTCAACTTGAT                                                 <  SRR4302543.1979102/94‑1 (MQ=60)
                                              TTATGCGTTCTCGGATTGGGGAAAAACGGTCATCTTGGGCTGAACGAGCCGGGAGAAAGCCTGCAACCGGCCTGCCATATCAGTCAACTTGATG                                                >  SRR4302543.4546444/1‑94 (MQ=60)
                                              TTATGCGTTCGCGGATTGGGGAAAAACGGTCATCTTGGGCTGAACGAGCCGGGAGAAAGCCTGCAACCGGCCTGCCATATCAGTCAACTTGATG                                                <  SRR4302543.5089946/94‑1 (MQ=60)
                                               TATGGGTTCTCGGTTTGGGGAAAAACGGCCATCTGGGGCTAAACGAGCCGGGAGAAAGCCTGCACCCGGCCTGCCATATCAGTCAACTTGATGC                                               <  SRR4302543.50118/94‑1 (MQ=60)
                                               TATGCGTTCTCGGATTGGGGAAAAACGGTCATCTTGGGCTGAACGAGCCGGGAGAAAGCCTGCAACCGGCCTGCCATATCAGTCAACTTGATGC                                               >  SRR4302543.1671874/1‑94 (MQ=60)
                                                ATGCGTTCTCGGATTGGGGAAAAACGGTCATCTTGGGCTGAACGAGCCGGGAGAAAGCCTGCAACCGGCCTGCCATATCAGTCAACTTGATGCC                                              >  SRR4302543.2959577/1‑94 (MQ=60)
                                                 TGGGTTCTCGGATGGGGAAAAAACGGTCATCTTGGGCTGAACGAGCCGGGAGAAAGCCTGCAACCGGCCTGCCATATCAGTCAACTTGATGCCA                                             <  SRR4302543.5953063/94‑1 (MQ=60)
                                                 TGCTTTCTCGGTTGGGGCAAAAACGGTCATCTTGGGCTGCACGAGCCGGGAGAAAGCCTGCAACCGGCCTGCCATATCAGTCAACTTGATGCCA                                             <  SRR4302543.2931237/94‑1 (MQ=60)
                                                    GTTCTCGGATTGGGGAAAAACGGTCATCTTGGGCTGAACGAGCCGGGAGAAAGCCTGCAACCGGCCTGCCATATCAGTCAACTTGATGCCAGAA                                          >  SRR4302543.930484/1‑94 (MQ=60)
                                                     TTCTCGGATTGGGGAAAAACGGTCATCTTGGGCTGAACGAGCCGGGAGAAAGCCTGCAACCGGCCTGCCATATCAGTCAACTTGATGCCAGAAC                                         >  SRR4302543.2415896/1‑94 (MQ=60)
                                                      TCTCGGATTGGGAAAAAGCGGTCATCTTGGGCTGAACGAGCCGGGAGAAAGCCTGCAACCGGCCTGCCATATCAGTCAACTTGATGCCAGAACA                                        <  SRR4302543.175174/94‑1 (MQ=60)
                                                       CTCGGGTTGGGGAAAAACGGTCACTTTGGGTTAATCGAGCCGGAAGAAAGCCTGCAACCGGCCTGCCATATCAGTCAACTTGATGCCAGAACAC                                       <  SRR4302543.1097031/94‑1 (MQ=60)
                                                        cgGGATTGGGAAAAAACGGTCATCTGGGGCTGAACGAGCCGGGAGAAAGCCTGCACCCGGCCTGCCATATCAGTCAACTTGATGCCAGAACACA                                      <  SRR4302543.4614768/92‑1 (MQ=60)
                                                        TGGGATGGGGAAAAAACGGTCATCTTGGGCTGAACGAGCCGGGAGAAAGCCTGCAACCGGCCTGCCATATCAGTCAACTTGATGCCAGAACACA                                      <  SRR4302543.6576564/94‑1 (MQ=60)
                                                         cgtattgggaaaaaccggccccctggcacgaAACGACCCGGGAAAAACCCTGCAACCGCCCTGCCATATCAGTCAACTTGATGCCAGAACACAG                                     <  SRR4302543.7001030/63‑1 (MQ=58)
                                                         GGGATGGGGGAAAAACGGTCATCTTGGGCTGAACGAGCCGGGAGAAAGCCTGCAACCGGCCTGCCATATCAGTCAACTTGATGCCAGAACACAG                                     <  SRR4302543.6359224/94‑1 (MQ=60)
                                                         CGGATTGGGGAAAAACGGTCATCTTGGGCTGAACGAGCCGGGAGAAAGCCTGCAACCGGCCTGCCATATCAGTCAACTTGATGCCAGAACACAG                                     >  SRR4302543.2978703/1‑94 (MQ=60)
                                                          GGATTGGGGAAAAACGGTCATCTTGGGCTGAACGAGCCGGGAGAAAGCCTGCAACCGGCCTGCCATATCAGTCAACTTGATGCCAGAACACAGC                                    >  SRR4302543.6101564/1‑94 (MQ=60)
                                                          GGATTGGGGAAAAACGGTCATCTTGGGCTGAACGAGCCGGGAGAAAGCCTGCAACCGGCCTGCCATATCAGTCAACTTGATGCCAGAACACAGC                                    >  SRR4302543.6101565/1‑94 (MQ=60)
                                                            ATTGGGGAAAAACGGTCATCTTGGGCTGAACGAGCCGGGAGAAAGCCTGCAACCGGCCTGCCATATCAGTCAACTTGATGCCAGAACACAGCAA                                  >  SRR4302543.4121645/1‑94 (MQ=60)
                                                             TGGGGGAAAAACGGTCATCTTGGGCTGAACGAGCCGGGAGAAAGCCTGCAACCGGCCTGCCATATCAGTCAACTTGATGCCAGAACACAGCAAC                                 <  SRR4302543.4567806/94‑1 (MQ=60)
                                                               GGGGAAAAACGGTCATCTTGGGCTGAACGAGCCGGGAGAAAGCCTGCAACCGGCCTGCCATATCAGTCAACTTGATGCCAGAACACAGACACAT                               >  SRR4302543.6347120/1‑94 (MQ=60)
                                                               GGGGAAAAACGGTCATCTGGGGCTGAACGAGCCGGGAGAAAGCCTGCAACCGGCCTGCCATATCAGTCAACTTGATGCCAGAACACAGCAACAT                               <  SRR4302543.6241811/94‑1 (MQ=60)
                                                                GGGAAAAACGGTCATCTTGGGCTGAACGAGCCGGGAGAAAGCCTGCAACCGGCCTGCCCTATCAGTCAACTTGATGCCAGAACACAGCAACATG                              >  SRR4302543.5597314/1‑94 (MQ=60)
                                                                GGGAAAAACGGTCATCTGGGGCTGAACGAGCCGGGAGAAAGCCTGCAACCGGCCTGCCATATCAGTCAACTTGATGCCAGAACACAGCAACATG                              <  SRR4302543.3773701/94‑1 (MQ=60)
                                                                 GGAAAAACGGTCATCTTGGGCTGAACGAGCCGGGAGAAAGCCTGCAACCGGCCTGCCATATCAGTCAACTTGATGCCAGAACACAGCAACATGA                             >  SRR4302543.1428841/1‑94 (MQ=60)
                                                                  GAAAACCGGTCATCTTGGGCGGAACGAGCCGGGAGAAAGCCTGCAACCGGCCTGCCATATCAGTCAACTTGATGCCAGAACACAGCAACATGAG                            <  SRR4302543.5805330/94‑1 (MQ=60)
                                                                  GAAAAAGGGTCATCTGGGGCTGACCGAGCCGGGAGAAAGCCTGCACCCGGCCTGCCATATCAGTCAACTTGATGCCAGAACACAGCAACATGAG                            <  SRR4302543.273014/94‑1 (MQ=60)
                                                                   AAAAACGGTCATCTTGGGCTGAACGAGCCGGGAGAAAGCCTGCAACCGGCCTGCCATATCAGTCAACTTGATGCCAGAACACAGCAACATGAGA                           >  SRR4302543.4594742/1‑94 (MQ=60)
                                                                    AAAACGGTCATCTTGGGCTGAACGAGCCGGGAGAAAGCCTGCAACCGGCCTGCCATATCAGTCAACTTGATGCCAGAACACAGCAACATGAGAT                          <  SRR4302543.6352266/94‑1 (MQ=60)
                                                                     AAGCGGTCGTCTGGGGCTGAACGAGCCGGGAGAAAGCCTGCAACCGGCCTGCCATATCAGTCAACTTGATGCCAGAACACAGCAACATGAGATG                         <  SRR4302543.3581425/94‑1 (MQ=60)
                                                                     AAACGTTCATCTTGGGCTGAACGAGCCGGGAGAAAGCCTGCAACCGGCCTGCCATATCAGTCAACTTGATGCCAGAACACAGCAACATGAGATG                         <  SRR4302543.3580813/94‑1 (MQ=60)
                                                                     AAACGGTCATCTTGGGCTGAACGAGCCGGGAGAAAGCCTGCAACCGGCCTGCCATATCAGTCAACTTGATGCCAGAACACAGCAACATGAGATG                         >  SRR4302543.3568968/1‑94 (MQ=60)
                                                                     AAACGGTCATCTTGGGCTGAACGAGCCGGGAGAAAGCCTGCAACCGGCCTGCCATATCAGTCAACTTGATGCCAGAACACAGCAACATGAGATG                         <  SRR4302543.7275260/94‑1 (MQ=60)
                                                                       ACGGTAATCTTGGGCGGAACGAGCCGGGAGAAAGCCTGCAACCGGCCTGCCATATCAGTCAACTTGATGCCAGAACACAGCAACATGAGATGTT                       <  SRR4302543.6491952/94‑1 (MQ=60)
                                                                        CGGTCATCTGGGGCTAAACGAGCCGGGAGAAAGCCTGCAACCGGCCTGCCATATCAGTCAACTTGATGCCAGAACACAGCAACATGAGATGTTA                      <  SRR4302543.5438643/94‑1 (MQ=60)
                                                                         GGTCATCTTGGGCTGAACGAGCCGGGAGAAAGCCTGCAACCGGCCTGCCATATCAGTCAACTTGATGCCAGAACACAGCAACATGAGATGTTAA                     >  SRR4302543.6440888/1‑94 (MQ=60)
                                                                          GTCATCTTGGGCTGAACGAGCCGGGAGAAAGCCTGCAACCGGCCTGCCATATCAGTCAACTTGATGCCAGAACACAGCAACATGAGATGTTAAA                    >  SRR4302543.3827224/1‑94 (MQ=60)
                                                                          GTCATCTGGGGCTGAACGAGCCGGGAGAAAGCCTGCAACCGGCCTGCCATATCAGTCAACTTGATGCCAGAACACAGCAACATGAGATGTTAAA                    <  SRR4302543.2196629/94‑1 (MQ=60)
                                                                          GTCATCTGGGGCGGAACGAGCCGGGAGAAAGCCTGCAACCGGCCTGCCATATCAGTCAACTTGATGCCAGAACACAGCAACATGAGATGTTAAA                    <  SRR4302543.2436296/94‑1 (MQ=60)
                                                                            gatctgggcCTGAACGAGCCGGGAGAAAGCCTGCAACCGGCCTGCCATATCAGTCAACTTGATGCCAGAACACAGCAACATGAGATGTTAAAAA                  <  SRR4302543.6273875/85‑1 (MQ=60)
                                                                            CATCTTGGGCTGAACGAGCCGGGAGAAAGCCTGCAACCGGCCTGCCATATCAGTCAACTTGATGCCAGAACACAGCAACATGAGATGTTAAAAA                  >  SRR4302543.2741383/1‑94 (MQ=60)
                                                                            CATCGTGGGCTGAACGAGCCGGGAGAAAGCCTGCAACCGGCCTGCCATATCAGTCAACTTGATGCCAGAACACAGCAACATGAGATGTTAAAAA                  <  SRR4302543.7651531/94‑1 (MQ=60)
                                                                             ATCTTGGGCTGAACGAGCCGGGAGAAAGCCTGCAACCGGCCTGCCATATCAGTCAACTTGATGCCAGCACACAGCAACATGAGATGTTAAAAAC                 >  SRR4302543.6968222/1‑94 (MQ=60)
                                                                             ATCTTGGGCTGAACGAGCCGGGAGAAAGCCTGCAACCGGCCTGCCATATCAGTCAACTTGATGCCAGAACACAGCAACATGAGATGTTAAAAAC                 >  SRR4302543.7497108/1‑94 (MQ=60)
                                                                             ATCTTGGGCTGAACGAGCCGGGAGAAAGCCTGCAACCGGCCTGCCATATCAGTCAACTTGATGCCAGAACACAGCAACATGAGATGTTAAAAAC                 >  SRR4302543.5620146/1‑94 (MQ=60)
                                                                             ATCTTGGGCTGAACGAGCCGGGAGAAAGCCTGCAACCGGCCTGCCATATCAGTCAACTTGATGCCAGAACACAGCAACATGAGATGTTAAAAAC                 >  SRR4302543.6968223/1‑94 (MQ=60)
                                                                             ATCTTGGGCTGAACGAGCCGGGAGAAAGCCTGCAACCGGCCTGCCATATCAGTCAACTTGATGCCAGAACACAGCAACATGAGATGTTAAAAAC                 >  SRR4302543.5922253/1‑94 (MQ=60)
                                                                             ATCTTGGGCGGATCGACCCGGGAGAAACCCTGCAACCGGCCTGCCATATCAGTCAACTTGATGCCAGAACACAGCAACATGAGATGTTAAAAAC                 <  SRR4302543.869111/94‑1 (MQ=60)
                                                                               ttgGGGCTGAGCGAGCCGGGAGAAAGCCTGCAACCGGCCTGCCATATCAGTCAACTTGATGCCAGAACACAGCAACATGAGATGTTAAAAACCG               <  SRR4302543.7349744/91‑1 (MQ=60)
                                                                               CTTGGGCTGAACGAGCCGGGAGAAAGCCTGCAACCGGCCTGCCATATCAGTCAACTTGATGCCAGAACACAGCAACATGAGATGTTAAAAACCG               >  SRR4302543.2000642/1‑94 (MQ=60)
                                                                               CTTGGGCTGAACGAGCCGGGAGAAAGCCTGCAACCGGCCTGCCATATCAGTCAACTTGATGCCAGAACACAGCAACATGAGATGTTAAAAACCG               >  SRR4302543.2023710/1‑94 (MQ=60)
                                                                                 TGGGCTGACCGAGCCGGGAAAAAGCCTGCAACCGGCCTGCCATATCAGTCAACTTGATGCCAGAACACAGCAACATGAGATGTTAAAAACCGCG             <  SRR4302543.7610133/94‑1 (MQ=60)
                                                                                 TGGGCTGAAGGAGCCGGGAGAAAGCCTGCAACCGGCCTGCCATATCAGTCAACTTGATGCCAGAACACAGCAACATGAGATGTTAAAAACCGCG             <  SRR4302543.911201/94‑1 (MQ=60)
                                                                                 GGGGCTGAACGAGCCGGGAGAAAGCCTGCAACCGGCCTGCCATATCAGTCAACTTGATGCCAGAACACAGCAACATGAGATGTTAAAAACCGCG             <  SRR4302543.7793178/94‑1 (MQ=60)
                                                                                 GGGGCTAAACGAGCCGGGAGAAAGCCTGCAACCGGCCTGCCATATCAGTCAACTTGATGCCAGAACACAGCAACATGAGATGTTAAAAACCGCG             <  SRR4302543.7793177/94‑1 (MQ=60)
                                                                                  GGGCTGAACGAGCCGGGAGAAAGCCTGCAACCGGCCTGCCATATCAGTCAACTTGATGCCAGAACACAGCAACATGAGATGTTAAAAACCGCGG            >  SRR4302543.155184/1‑94 (MQ=60)
                                                                                  GGGCTAAGCGAGCCGGGAGAAAGCCTGCAACCGGCCTGCCATATCAGTCAACTTGATGCCAGAACACAGCAACATGAGATGTTAAAAACCGCGG            <  SRR4302543.4313414/94‑1 (MQ=60)
                                                                                     CTGAACGAGCCGGGAGAAAGCCTGCAACCGGCCTGCCATATCAGTCAACTTGATGCCAGAACACAGCAACATGAGATGTTAAAAACCGCGGGTC         >  SRR4302543.1318919/1‑94 (MQ=60)
                                                                                      TGAACGAGCGGGGAGAAAGCCTGCAACCGGCCTGCCATATCAGTCAACTTGATGCCAGAACACAGCAACATGAGATGTTAAAAACCGCGGGTCG        <  SRR4302543.7146067/94‑1 (MQ=60)
                                                                                      TGAACGAGCCGGGAGAAAGGCTGCAACCGGCCTGCCATATCAGTCAACTTGATGCCAGAACACAGCAACATGAGATGTTAAAAACCGCGGGTCC        >  SRR4302543.3524452/1‑94 (MQ=60)
                                                                                      TGAACGAGCCGGGAGAAAGCCTGCAACCGGCCTGCCATATCAGTCAACTTGATGCCAGAACACAGCAACATGAGATGTTAAAAACCGCGGGTCG        >  SRR4302543.1207989/1‑94 (MQ=60)
                                                                                      TGAACGAGCCGGGAGAAAGCCTGCAACCGGCCTGCCATATCAGTCAACTTGATGCCAGAACACAGCAACATGAGATGTTAAAAACCGCGGGTCG        >  SRR4302543.7905997/1‑94 (MQ=60)
                                                                                      TGAACGAGCCGGGAGAAACCCTGCACCCGGCCTGCCATATCAGTCAACTTGATGCCAGAACACAGCAACATGAGATGTTAAAAACCGCGGGTCG        <  SRR4302543.5813277/94‑1 (MQ=60)
                                                                                        AACGAGCCGGGAGAAAGCCTGCAACCGGCCTGCCATATCAGTCAACTTGATGCCAGAACACAGCAACATGAGATGTTAAAAACCGCGGGTCGCC      >  SRR4302543.5908724/1‑94 (MQ=60)
                                                                                        AACGAGCCGGGAGAAAGCCTGCAACCGGCCTGCCATATCAGTCAACTTGATGCCAGAACACAGCAACATGAGATGTTAAAAACCGCGGGTCGCC      >  SRR4302543.4375432/1‑94 (MQ=60)
                                                                                          CGAGCCGGGAGAAAGCCTGCAACCGGCCTGCCATATCAGTCAACTTGATGCCAGAACACAGCAACATGAGATGTTAAAAACCGCGGGTCGCCCC    >  SRR4302543.2576152/1‑94 (MQ=60)
                                                                                          CGAGCCGGGAGAAAGCCTGCAACCGGCCTGCCATATCAGTCAACTTGATGCCAGAACACAGCAACATGAGATGTTAAAAACCGCGGGTCGCCCC    >  SRR4302543.2839928/1‑94 (MQ=60)
                                                                                           GAGCCGGGAGAAAGCCTGCACCCGGCCTGCCATATCAGTCAACTTGATGCCAGAACACAGCAACATGAGATGTTAAAAACCGCGGGTCGCCCCG   <  SRR4302543.6733692/94‑1 (MQ=60)
                                                                                            AGCCGGGAGAAAGCCTGCAACCGGCCTGCCATATCAGTCAACTTGATGCCAGAACACAGCAACATGAGATGTTAAAAACCGCGGGTCGCCCCGT  >  SRR4302543.2683806/1‑94 (MQ=60)
                                                                                             |                                                                                            
GTGCGAACGGGTAACGAACCTGATTGCGCGCAAAGGCGGTCTGGATTTATGCGTTCTCGGATTGGGGAAAAACGGTCATCTTGGGCTGAACGAACCGGGAGAAAGCCTGCAACCGGCCTGCCATATCAGTCAACTTGATGCCAGAACACAGCAACATGAGATGTTAAAAACCGCGGGTCGCCCCGT  >  NC_000913/3284300‑3284485

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑