Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 1696 279.3 15874187 97.8% 15524954 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,309,164 A→G R54R (CGT→CGC)  rpsO ← 30S ribosomal subunit protein S15

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,309,1640AG99.4% 1073.8 / ‑3.9 345R54R (CGT→CGC) rpsO30S ribosomal subunit protein S15
Reads supporting (aligned to +/- strand):  ref base A (2/0);  new base G (173/170);  total (175/170)
Fisher's exact test for biased strand distribution p-value = 4.99e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.49e-01

Only 100 of 366 total aligned reads displayed.

CGCTCGATGAGCTGGGTGTAACGTGCTACGTCTTTACGTTTCAGGTAGTCGAGCAGTTTACGACGCTGAGAAACCATGCGCAGCAGACCACGACGGCTGTGGTGATCTTTTTTGTGCTCTGCAAAGTGGCCCTGCAGGTGGTTGATCTGTGCAGTCAGCAGTGCTACCTGAACTTCGGT  >  NC_000913/3309075‑3309253
                                                                                         |                                                                                         
cgcTCGATGAGCTGGGTGTAACGTGCTACGTCTTTACGTTTCAGGTAGTCGAGCAGTTTACGACGCTGAGAAACCATGCGCAGCAGACCgcga                                                                                        <  2:385477/93‑1 (MQ=255)
cgcTCGATGAGCTGGGTGTAACGTGCTACGTCTTTACGTTTCAGGTAGTCGAGCAGTTTACGACGCTGAGAAACCATGCGCAGCAGACCgcga                                                                                        <  1:7858798/93‑1 (MQ=255)
cgcTCGATGAGCTGGGTGTAACGTGCTACGTCTTTACGTTTCAGGTAGTCGAGCAGTTTACGACGCTGAGAAACCATGCGCAGCAGACCgcga                                                                                        <  2:4877560/93‑1 (MQ=255)
cgcTCGATGAGCTGGGTGTAACGTGCTACGTCTTTACGTTTCAGGTAGTCCAGCAGTTTACGACGCTGAGAAACCATGCGCAGCAGACCgcga                                                                                        <  1:6202917/93‑1 (MQ=255)
 gcTCGATGAGCTGGGTGTAACGTGCTACGTCTTTACGTTTCAGGTAGTCGAGCAGTTTACGACGCTGAGAAACCATGCGCAGCAGACCgcgac                                                                                       <  1:276355/93‑1 (MQ=255)
   tggATGAGCTGGGTGTAACGTGCTACGTCTTTACGTTTCAGGTAGTCGAGCAGTTTACGACGCTGAGAAACCATGCGCAGCAGACCGCGACgg                                                                                     <  1:5633696/91‑1 (MQ=255)
   tCGATGAGCTGGGTGTAACGTGCTACGTCTTTACGTTTCAGGTAGTCGAGCAGTTTACGACGCTGAGAAACCATGCGCAGCAGACCGCGACgg                                                                                     >  2:6246979/1‑93 (MQ=255)
    cGATGAGCTGGGTGTAACGTGCTACGTCTTTACGTTTCAGGTAGTCGAGCAGTTTACGACGCTGAGAAACCATGCGCAGCAGACCGCGACGGc                                                                                    <  1:7583611/93‑1 (MQ=255)
     gATGAGCTGGGTGTAACGTGCTACGTCTTTACGTTTCAGGTAGTCGAGCAGTTTACGACGCTGAGAAACCATGCGCAGCAGACCGCGACGGCt                                                                                   >  2:3341369/1‑93 (MQ=255)
     gATGAGCTGGGTGTAACGTGCTACGTCTTTACGTTTCAGGTAGTCGAGCAGTTTACGACGCTGAGAAACCATGCGCAGCAGACCGCGACGGCt                                                                                   <  1:937757/93‑1 (MQ=255)
     gATGAGCTGGGTGTAACGTGCTACGTCTTTACGTTTCAGGTAGTCGAGCAGTTTACGACGCTGAGAAACCATGCGCAGCAGACCGCGACGGCt                                                                                   >  1:7721080/1‑93 (MQ=255)
     gATGAGCTGGGTGTAACGTGCTACGTCTTTACGTTTCAGGTAGTCGAGCAGTTTACGACGCTGAGAAACCATGCGCAGCAGACCGCGACGGCt                                                                                   <  1:5920489/93‑1 (MQ=255)
      aTGAGCTGGGTGTAACGTGCTACGTCTTTACGTTTCAGGTAGTCGAGCAGTTTACGACGCTGAGAAACCATGCGCAGCAGACCGCGACGGCtg                                                                                  >  2:5975493/1‑93 (MQ=255)
       tGAGCTGGGTGTAACGTGCTACGTCTTTACGTTTCAGGTAGTCGAGCAGTTTACGACGCTGAGAAACCATGCGCAGCAGACCGCGACGGCtgt                                                                                 <  1:7141976/93‑1 (MQ=255)
       tGAGCTGGGTGTAACGTGCTACGTCTTTACGTTTCAGGTAGTCGAGCAGTTTACGACGCTGAGAAACCATGCGCAGCAGACCGCGACGGCtgt                                                                                 >  1:522566/1‑93 (MQ=255)
       tGAGCTGGGTGTAACGTGCTACGTCTTTACGTTTCAGGTAGTCGAGCAGTTTACGACGCTGAGAAACCATGCGCAGCAGACCGCGACGGCtgt                                                                                 <  2:1465428/93‑1 (MQ=255)
       tGAGCTGGGTGTAACGTGCTACGTCTTTACGTTTCAGGTAGTCGAGCAGTTTACGACGCTGAGAAACCATGCGCAGCAGACCGCGACGGCtgt                                                                                 <  1:2284952/93‑1 (MQ=255)
       tGAGCTGGGTGTAACGTGCTACGTCTTTACGTTTCAGGTAGTCGAGCAGTTTACGACGCTGAGAAACCATGCGCAGCAGACCGCGACGGCtgt                                                                                 >  1:6257470/1‑93 (MQ=255)
       tGAGCTGGGTGTAACGTGCTACGTCTTTACGTTTCAGGTAGTCGAGCAGTTTACGACGCTGAGAAACCATGCGCAGCAGACCGCGACGGCtgt                                                                                 >  2:7314000/1‑93 (MQ=255)
       tGAGCTGGGTGTAACGTGCTACGTCTTTACGTTTCAGGTAGTCGAGCAGTTTACGACGCTGAGAAACCATGCGCAGCAGACCGCGACGGCtgt                                                                                 <  1:7428709/93‑1 (MQ=255)
          gCTGGGTGTAACGTGCTACGTCTTTACGTTTCAGGTAGTCGAGCAGTTTACGACGCTGAGAAACCATGCGCAGCAGACCGCGACGGCTgtggt                                                                              >  1:2583419/1‑93 (MQ=255)
          gCTGGGTGTAACGTGCTACGTCTTTACGTTTCAGGTAGTCGAGCAGTTTACGACGCTGAGAAACCATGCGCAGCAGACCGCGACGGCTgtggt                                                                              <  2:3986329/93‑1 (MQ=255)
          gCTGGGTGTAACGTGCTACGTCTTTACGTTTCAGGTAGTCGAGCAGTTTACGACGCTGAGAAACCATGCGCAGCAGACCGCGACGGCTgtggt                                                                              <  2:3594550/93‑1 (MQ=255)
          gCTGGGTGTAACGTGCTACGTCTTTACGTTTCAGGTAGTCGAGCAGTTTACGACGCTGAGAAACCATGCGCAGCAGACCGCGACGGCTgtggg                                                                              >  2:588425/1‑92 (MQ=255)
          gCTGGGTGTAACGTGCTACGTCTTTACGTTTCAGGTAGTCGAGCAGTTTACGACGCTGAGAAACCATGCGCAGCAGACCGCGACGGCTgtggg                                                                              >  2:217916/1‑92 (MQ=255)
           cTGGGTGTAACGTGCTACGTCTTTACGTTTCAGGTAGTCGAGCAGTTTACGACGCTGAGAAACCATGCGCAGCAGACCGCGACGGCTgtggtg                                                                             >  1:1060388/1‑93 (MQ=255)
            tGGGTGTAACGTGCTACGTCTTTACGTTTCAGGTAGTCGAGCAGTTTACGACGCTGAGAAACCATGCGCAGCAGACCGCGACGGCTGTGGTGa                                                                            >  2:1823667/1‑93 (MQ=255)
              ggTGTAACGTGCTACGTCTTTACGTTTCAGGTAGTCGAGCAGTTTACGACGCTGAGAAACCATGCGCAGCAGACCGCGACGGCTGTGGTGATc                                                                          <  2:6508879/93‑1 (MQ=255)
               gtgtAACGTGCTACGTCTTTACGTTTCAGGTAGTCGAGCAGTTTACGACGCTGAGAAACCATGCGCAGCAGACCGCGACGGCTGTGGTGATCt                                                                         >  2:2415506/1‑93 (MQ=255)
                tgtAACGTGCTACGTCTTTACGTTTCAGGTAGTCGAGCAGTTTACGACGCTGAGAAACCATGCGCAGCAGACCGCGACGGCTGTGGTGATCtt                                                                        <  1:4906959/93‑1 (MQ=255)
                 gtAACGTGCTACGTCTTTACGTTTCAGGTAGTCGAGCAGTTTACGACGCTGAGAAACCATGCGCAGCAGACCGCGACGGCTGTGGTGATCttt                                                                       <  2:4438104/93‑1 (MQ=255)
                  tAACGTGCTACGTCTTTACGTTTCAGGTAGTCGAGCAGTTTACGACGCTGAGAAACCATGCGCAGCAGACCGCGACGGCTGTGGTGATCtttt                                                                      >  1:1856437/1‑93 (MQ=255)
                   aaCGTGCTACGTCTTTACGTTTCAGGTAGTCGAGCAGTTTACGACGCTGAGAAACCATGCGCAGCAGACCGCGACGGCTGTGGTGATCttttt                                                                     >  1:1354356/1‑93 (MQ=255)
                   aaCGTGCTACGTCTTTACGTTTCAGGTAGTCGAGCAGTTTACGACGCTGAGAAACCATGCGCAGCAGACCGCGACGGCTGTGGTGATCttttt                                                                     >  1:5112285/1‑93 (MQ=255)
                   aaCGTGCTACGTCTTTACGTTTCAGGTAGTCGAGCAGTTTACGACGCTGAGAAACCATGCGCAGCAGACCGCGACGGCTGTGGTGATCttttt                                                                     <  2:5243725/93‑1 (MQ=255)
                   aaCGTGCTACGTCTTTACGTTTCAGGTAGTCGAGCAGTTTACGACGCTGAGAAACCATGCGCAGCAGACCGCGACGGCTGTGGTGATCttttt                                                                     >  2:5450633/1‑93 (MQ=255)
                    aCGTGCTACGTCTTTACGTTTCAGGTAGTCGAGCAGTTTACGACGCTGAGAAACCATGCGCAGCAGACCGCGACGGCTGTGGTGATCtttttt                                                                    >  1:3766850/1‑93 (MQ=255)
                    aCGTGCTACGTCTTTACGTTTCAGGTAGTCGAGCAGTTTACGACGCTGAGAAACCATGCGCAGCAGACCGCGACGGCTGTGGTGATCtttttt                                                                    <  2:466712/93‑1 (MQ=255)
                      gTGCTACGTCTTTACGTTTCAGGTAGTCGAGCAGTTTACGACGCTGAGAAACCATGCGCAGCAGACCGCGACGGCTGTGGTGATCTTTTTtgt                                                                  >  1:4046805/1‑93 (MQ=255)
                       tGCTACGTCTTTACGTTTCAGGTAGTCGTGCAGTTTACGACGCTGAGAAACCATGCGCAGCAGACCGCGATGGCTGTGGTGATCTTTTTtgtg                                                                 <  2:2536497/93‑1 (MQ=255)
                       tGCTACGTCTTTACGTTTCAGGTAGTCGAGCAGTTTACGACGCTGAGAAACCATGCGCAGCAGACCGCGACGGCTGTGGTGATCTTTTTtgtg                                                                 <  1:4812499/93‑1 (MQ=255)
                        gCTACGTCTTTACGTTTCAGGTAGTCGAGCAGTTTACGACGCTGAGAAACCATGCGCAGCAGACCGCGACGGCTGTGGTGATCTTTTTtgtat                                                                >  1:3875664/1‑91 (MQ=255)
                         cTACGTCTTTACGTTTCAGGTAGTCGAGCAGTTTACGACGCTGAGAAACCATGCGCAGCAGACCGCGACGGCTGTGGTGATCTTTTTTGTGct                                                               >  2:739288/1‑93 (MQ=255)
                          tACGTCTTTACGTTTCAGGTAGTCGAGCAGTTTACGACGCTGAGAAACCATGCGCAGCAGACCGCGACGGCTGTGGTGATCTTTTTTGTGctc                                                              <  1:1094208/93‑1 (MQ=255)
                          tACGTCTTTACGTTTCAGGTAGTCGAGCAGTTTACGACGCTGAGAAACCATGCGACGCAGAACGCGACGGCTGTGGTGATCTTTTTTGTGctt                                                              >  1:3291248/1‑92 (MQ=255)
                           aCGTCTTTACGTTTCAGGTAGTCGAGCAGTTTACGACGCTGAGAAACCATGCGCAGCAGACCGCGACGGCTGTGGTGATCTTTTTTGTGctct                                                             <  2:7697504/93‑1 (MQ=255)
                             gTCTTTACGTTTCAGGTAGTCGAGCAGTTTACGACGCTGAGAAACCATGCGCAGCAGACCGCGACGGCTGTGGTGATCTTTTTTGTGCTCTGc                                                           >  1:6832661/1‑93 (MQ=255)
                             gTCTTTACGTTTCAGGTAGTCGAGCAGTTTACGACGCTGAGAAACCATGCGCAGCAGACCGCGACGGCTGTGGTGATCTTTTTTGTGCTCTGc                                                           <  1:6917876/93‑1 (MQ=255)
                              tCTTTACGTTTCAGGTAGTCGAGCAGTTTACGACGCTGAGAAACCATGCGCAGCAGACCGCGACGGCTGTGGTGATCTTTTTTGTGCTCTGCa                                                          >  1:1531865/1‑93 (MQ=255)
                                tttACGTTTCAGGTAGTCGAGCAGTTTACGACGCTGATAAACCATGCGCAGCAGACCGCGACGGCTGTGGTGATCTTTTTTGTGCTCTGCaaa                                                        <  1:733941/93‑1 (MQ=255)
                                tttACGTTTCAGGTAGTCGAGCAGTTTACGACGCTGAGAAACCATGCGCAGCAGACCGCGACGGCTGTGGTGATCTTTTTTGTGCTCTGCaaa                                                        >  1:4703247/1‑93 (MQ=255)
                                tttACGTTTCAGGTAGTCGAGCAGTTTACGACGCTGAGAAACCATGCGCAGCAGACCGCGACGGCTGTGGTGATCTTTTTTGTGCTCTGCaaa                                                        >  1:3941649/1‑93 (MQ=255)
                                tttACGTTTCAGGTAGTCGAGCAGTTTACGACGCTGAGAAACCATGCGCAGCAGACCGCGACGGCTGTGGTGATCTTTTTTGTGCTCTGCaaa                                                        >  2:1068311/1‑93 (MQ=255)
                                 ttACGTTTCAGGTAGTCGAGCAGTTTACGACGCTGAGAAACCATGCGCAGCAGACCGCGACGGCTGTGGTGATCTTTTTTGTGCTCTGCAAAg                                                       >  1:3603852/1‑93 (MQ=255)
                                 ttACGTTTCAGGTAGTCGAGCAGTTTACGACGCTGAGAAACCATGCGCAGCAGACCGCGACGGCTGTGGTGATCTTTTTTGTGCTCTGCAAAg                                                       >  2:2803588/1‑93 (MQ=255)
                                  tACGTTTCAGGTAGTCGAGCAGTTTACGACGCTGAGAAACCATGCGCAGCAGACCGCGACGGCTGTGGTGATCTTTTTTGTGCTCTGCAAAGt                                                      >  2:6795951/1‑93 (MQ=255)
                                    cGTTTCAGGTAGTCGAGCAGTTTACGACGCTGAGAAACCATGCGCAGCAGACCGCGACGGCTGTGGTGATCTTTTTTGTGCTCTGCAAAGTgg                                                    >  2:569701/1‑93 (MQ=255)
                                      tttCAGGTAGTCGAGCAGTTTACGACGCTGAGAAACCATGCGCAGCAGACCGCGACGGCTGTGGTGATCTTTTTTGTGCTCTGCAAAGTGGcc                                                  >  1:7916210/1‑93 (MQ=255)
                                      tttCAGGTAGTCGAGCAGTTTACGACGCTGAGAAACCATGCGCAGCAGACCGCGACGGCTGTGGTGATCTTTTTTGTGCTCTGCAAAGTGGcc                                                  <  1:6403479/93‑1 (MQ=255)
                                      tttCAGGTAGTCGAGCAGTTTACGACGCTGAGAAACCATGCGCAGCAGACCGCGACGGCTGTGGTGATCTTTTTTGTGCTCTGCAAAGTGGcc                                                  <  2:3136240/93‑1 (MQ=255)
                                      tttCAGGTAGTCGAGCAGTTTACGACGCTGAGAAACCATGCGCAGCAGACCGCGACGGCTGTGGTGATCTTTTTTGTGCTCTGCAAAGTGGcc                                                  <  2:7069335/93‑1 (MQ=255)
                                        tCAGGTAGTCGAGCAGTTTACGACGCTGAGAAACCATGCGCAGCAGACCGCGACGGCTGTGGTGATCTTTTTTGTGCTCTGCAAAGTGGCCCt                                                <  1:7297028/93‑1 (MQ=255)
                                           ggTAGTCGAGCAGTTTACGACGCTGAGAAACCATGCGCAGCAGACCGCGACGGCTGTGGTGATCTTTTTTGTGCTCTGCAAAGTGGCCCTGCa                                             <  1:7190578/93‑1 (MQ=255)
                                           ggTAGTCGAGCAGTTTACGACGCTGAGAAACCATGCGCAGCAGACCGCGACGGCTGTGGTGATCTTTTTTGTGCTCTGCAAAGTGGCCCTGCa                                             >  1:7750966/1‑93 (MQ=255)
                                            gTAGTCGAGCAGTTTACGACGCTGAGAAACCATGCGCAGCAGACCGCGACGGCTGTGGTGATCTTTTTTGTGCTCTGCAAAGTGGCCCTGCAg                                            <  1:6064796/93‑1 (MQ=255)
                                             tAGTCGAGCAGTTTACGACGCTGAGAAACCATGCGCAGCAGACCGCGACGGCTGTGGTGATCTTTTTTGTGCTCTGCAAAGTGGCCCTGCAgg                                           >  2:6182597/1‑93 (MQ=255)
                                              aGTCGAGCAGTTTACGACGCTGAGAAACCATGCGCAGCAGACCGCGACGGCTGTGGTGATCTTTTTTGTGCTCTGCAAAGTGGCCCTGCAggt                                          <  2:3111969/93‑1 (MQ=255)
                                                 cGAGCAGTTTACGACGCTGAGAAACCATGCGCAGCAGACCGCGACGGCTGTGGTGATCTTTTTTGTGCTCTGCAAAGTGGCCCTGCAggtggt                                       >  2:1601221/1‑93 (MQ=255)
                                                 cGAGCAGTTTACGACGCTGAGAAACCATGCGCAGCAGACCGCGACGGCTGTGGTGATCTTTTTTGTGCTCTGCAAAGTGGCCCTGCAggtggt                                       >  1:2960937/1‑93 (MQ=255)
                                                    gCAGTTTACGACGCTGAGAAACCATGCGCAGCAGACCGCGACGGCTGTGGTGATCTTTTTTGTGCTCTGCAAAGTGGCCCTGCAGGTGGTTGa                                    >  1:1238827/1‑93 (MQ=255)
                                                      aGTTTACGACGCTGAGAAACCATGCGCAGCAGACCGCGACGGCTGTGGTGATCTTTTTTGTGCTCTGCAAAGTGGCCCTGCAGGTGGTTGATc                                  <  1:6445169/93‑1 (MQ=255)
                                                      aGTTTACGACGCTGAGAAACCATGCGCAGCAGACCGCGACGGCTGTGGTGATCTTTTTTGTGCTCTGCAAAGTGGCCCTGCAGGTGGTTGATc                                  <  2:1978938/93‑1 (MQ=255)
                                                      aGTTTACGACGCTGAGAAACCATGCGCAGCAGACCGCGACGGCTGTGGTGATCTTTTTTGTGCTCTGCAAAGTGGCCCTGCAGGTGGTTGATc                                  <  1:5295376/93‑1 (MQ=255)
                                                      aGTTTACGACGCTGAGAAACCATGCGCAGCAGACCGCGACGGCTGTGGTGATCTTTTTTGTGCTCTGCAAAGTGGCCCTGCAGGTGGTTGATc                                  <  1:2458254/93‑1 (MQ=255)
                                                       gTTTACGACGCTGAGAAACCATGCGCAGCAGACCGCGACGGCTGTGGTGATCTTTTTTGTGCTCTGCAAAGTGGCCCTGCAGGTGGTTGATCt                                 <  1:3000661/93‑1 (MQ=255)
                                                        tttACGACGCTGAGAAACCATGCGCAGCAGACCGCGACGGCTGTGGTGATCTTTTTTGTGCTCTGCAAAGTGGCCCTGCAGGTGGTTGATCtg                                >  2:818670/1‑93 (MQ=255)
                                                        tttACGACGCTGAGAAACCATGCGCAGCAGACCGCGACGGCTGTGGTGATCTTTTTTGTGCTCTGCAAAGTGGCCCTGCAGGTGGTTGATCtg                                >  2:2656810/1‑93 (MQ=255)
                                                         ttACGACGCTGAGAAACCATGCGCAGCAGACCGCGACGGCTGTGGTGATCTTTTTTGTGCTCTGCAAAGTGGCCCTGCAGGTGGTTGATCtgt                               >  1:4266263/1‑93 (MQ=255)
                                                            cgacgCTGAGAAACCATGCGCAGCAGACCGCGACGGCTGTGGTGATCTTTTTTGTGCTCTGCAAAGTGGCCCTGCAGGTGGTTGATCTGTGCa                            <  1:5695058/93‑1 (MQ=255)
                                                                  tGAGAAACCATGCGCAGCAGACCGCGACGGCTGTGGTGATCTTTTTTGTGCTCTGCAAAGTGGCCCTGCAGGTGGTTGATCTGTGCAGTcagc                      >  1:1429751/1‑93 (MQ=255)
                                                                  tGAGAAACCATGCGCAGCAGACCGCGACGGCTGTGGTGATCTTTTTTGTGCTCTGCAAAGTGGCCCTGCAGGTGGTTGATCTGTGCAGTcagc                      <  2:5290836/93‑1 (MQ=255)
                                                                  tGAGAAACCATGCGCAGCAGACCGCGACGGCTGTGGTGATCTTTTTTGTGCTCTGCAAAGTGGCCCTGCAGGAGGTTGATCTGTGCAGTcagc                      >  2:4067975/1‑93 (MQ=255)
                                                                   gagaAACCATGCGCAGCAGACCGCGACGGCTGTGGTGATCTTTTTTGTGCTCTGCAAAGTGGCCCTGCAGGTGGTTGATCTGTGCAGTcagca                     >  1:4320631/1‑93 (MQ=255)
                                                                    agaAACCATGCGCAGCAGACCGCGACGGCTGTGGTGATCTTTTTTGTGCTCTGCAAAGTGGCCCTGCAGGTGGTTGATCTGTGCAGTcagcag                    <  2:4496083/93‑1 (MQ=255)
                                                                     gaAACCATGCGCAGCAGACCGCGACGGCTGTGGTGATCTTTTTTGTGCTCTGCAAAGTGGCCCTGCAGGTGGTTGATCTGTGCAGTCAGCAGt                   >  1:3160249/1‑93 (MQ=255)
                                                                      aaaCCATGCGCAGCAGACCGCGACGGCTGTGGTGATCTTTTTTGTGCTCTGCAAAGTGGCCCTGCAGGTGGTTGATCTGTGCAGTCAGCAGTg                  <  2:3952405/93‑1 (MQ=255)
                                                                       aaCCATGCGCAGCAGACCGCGACGGCTGTGGTGATCTTTTTTGTGCTCTGCAAAGTGGCCCTGCAGGTGGTTGATCTGTGCAGTCAGCAGTGc                 >  2:4771509/1‑93 (MQ=255)
                                                                         ccATGCGCAGCAGACCGCGACGGCTGTGGTGATCTTTTTTGTGCTCTGCAAAGTGGCCCTGCAGGTGGTTGATCTGTGCAGTCAGCAGTGCTa               >  1:2223346/1‑93 (MQ=255)
                                                                          cATGCGCAGCAGTCCGCGACGGCTGTGGTGATCTTTTTTGTGCTCTGCAAAGTGGCCCTGCAGGTGGTTGATCTGTGCAGTCAGCAGTGCTAc              <  1:1401177/93‑1 (MQ=255)
                                                                           aTGCGCAGCAGACCGCGACGGCTGTGGTGATCTTTTTTGTGCTCTGCAAAGTGGCCCTGCAGGTGGTTGATCTGTGCAGTCAGCAGTGCTAcc             >  2:2989020/1‑93 (MQ=255)
                                                                           aTGCGCAGCAGACCGCGACGGCTGTGGTGATCTTTTTTGTGCTCTGCAAAGTGGCCCTGCAGGTGGTTGATCTGTGCAGTCAGCAGTGCTAcc             >  2:6930190/1‑93 (MQ=255)
                                                                            tGCGCAGCAGACCGCGACGGCTGTGGTGATCTTTTTTGTGCTCTGCAAAGTGGCCCTGCAGGTGGTTGATCTGTGCAGTCAGCAGTGCTACCt            >  1:4618808/1‑93 (MQ=255)
                                                                             gcgcAGCAGACCGCGACGGCTGTGGTGATCTTTTTTGTGCTCTGCAAAGTGGCCCTGCAGGTGGTTGATCTGTGCAGTCAGCAGTGCTACCTg           <  2:7143304/93‑1 (MQ=255)
                                                                                  gcagACCGCGACGGCTGTGGTGATCTTTTTTGTGCTCTGCAAAGTGGCCCTGCAGGTGGTTGATCTGTGCAGTCAGCAGTGCTACCTGaa         <  2:1360209/90‑1 (MQ=255)
                                                                                  gcagACAGCGACGGCTGTGGTGATCTTTTTTGTGCTCTGCAAAGTGGCCCTGCAGGTGGTTGATCTGTGCAGTCAGCAGTGCTACCTGAACtt      <  2:609989/93‑1 (MQ=255)
                                                                                   cagACCGCGACGGCTGTGGTGATCTTTTTTGTGCTCTGCAAAGTGGCCCTGCAGGTGGTTGATCTGTGCAGTCAGCAGTGCTACCTGAACTTc     <  1:31/93‑1 (MQ=255)
                                                                                     gACCGCGACGGCTGTGGTGATCTTTTTTGTGCTCTGCAAAGTGGCCCTGCAGGTGGTTGATCTGTGCAGTCAGCAGTGCTACCTGAACTTCgg   <  1:3435487/93‑1 (MQ=255)
                                                                                     gACCGCGACGGCTGTGGTGATCTTTTTTGTGCTCTGCAAAGTGGCCCTGCAGGTGGTTGATCTGTGCAGTCAGCAGTGCTACCTGAACTTCgg   <  2:686550/93‑1 (MQ=255)
                                                                                     gACCGCGACGGCTGTGGTGATCTTTTTTGTGCTCTGCAAAGTGGCCCTGCAGGTGGTTGATCTGTGCAGTCAGCAGTGCTACCTGAACTTCgg   <  1:1185273/93‑1 (MQ=255)
                                                                                      aCCGCGACGGCTGTGGTGATCTTTTTTGTGCTCTGCAAAGTGGCCCTGCAGGTGGTTGATCTGTGCAGTCAGCAGTGCTACCTGAACTTCGGt  >  1:5480787/1‑93 (MQ=255)
                                                                                         |                                                                                         
CGCTCGATGAGCTGGGTGTAACGTGCTACGTCTTTACGTTTCAGGTAGTCGAGCAGTTTACGACGCTGAGAAACCATGCGCAGCAGACCACGACGGCTGTGGTGATCTTTTTTGTGCTCTGCAAAGTGGCCCTGCAGGTGGTTGATCTGTGCAGTCAGCAGTGCTACCTGAACTTCGGT  >  NC_000913/3309075‑3309253

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output Only 200 of 212 total aligned reads displayed.
CAGGCGCTCGATGAGCTGGGTGTAACGTGCTACGTCTTTACGTTTCAGGTAGTCGAGCAGTTTACGACGCTGAGAAACCATGCGCAGCAGACCACGACGGCTGTGGTGATCTTTTTTGTGCTCTGCAAAGTGGCCCTGCAGGTGGTTGATCTGTGCAGTCAGCAGTGCTACCTGAACTTCGGTAGA  >  NC_000913/3309071‑3309256
                                                                                             |                                                                                            
CAGGCGCTCGATGAGCTGGGTGTAACGTGCTACGTCTTTACGTTTCAGGTAGTCGAGCAGTTTACGACGCTGAGAAACCATGCGCAGCAGACCG                                                                                              <  SRR4302543.7254612/94‑1 (MQ=60)
CAGGCGCTCGATGAGCTGGGTGTAACGTGCTACGTCTTTACGTTTCAGGTAGTCGAGCAGTTTACGACGCTGAGAAACCATGCGCAGCAGACCG                                                                                              >  SRR4302543.6823373/1‑94 (MQ=60)
CAGGCGCTCGATGAGCTGGGTGTAACGTGCTACGTCTTTACGTTTCAGGTAGTCGAGCAGTTTACGACGCTGAGAAACCATGCGCAGCAGACCG                                                                                              >  SRR4302543.7807240/1‑94 (MQ=60)
 AGGCGCTCGATGAGCTGGGTGTAACGTGCTACGTCTTTACGTTTCAGGTAGTCGAGCAGTTTACGACGCTGAGAAACCATGCGCAGCAGACCGC                                                                                             <  SRR4302543.7607367/94‑1 (MQ=60)
 AGGCGCTCGATGAGCTGGGTGTAACGTGCTACGTCTTTACGTTTCAGGTAGTCGAGCAGTTTACGACGCTGAGAAACCATGCGCAGCAGACCGC                                                                                             >  SRR4302543.1268033/1‑94 (MQ=60)
 AGGCGCTCGATGAGCTGGGTGTAACGTGCTACGTCTTTACGTTTCAGGTAGTCGAGCAGTTTACGACGCTGAGAAACCATGCGCAGCAGACCGC                                                                                             <  SRR4302543.3180422/94‑1 (MQ=60)
    CGCTCGATGAGCTGGGTGTAACGTGCTACGTCTTTACGTTTCAGGTAGTCGAGCAGTTTACGACGCTGAGAAACCATGCGCAGCAGACCGCGAC                                                                                          <  SRR4302543.7509621/94‑1 (MQ=60)
    CGCTCGATGAGCTGGGTGTAACGTGCTACGTCTTTACGTTTCAGGTAGTCGAGCAGTTTACGACGCTGAGAAACCATGCGCAGCAGACCGCGAC                                                                                          <  SRR4302543.5303242/94‑1 (MQ=60)
    CGCTCGATGAGCTGGGTGTAACGTGCTACGTCTTTACGTTTCAGGTAGTCGAGCAGTTTACGACGCTGAGAAACCATGCGCAGCAGACCGCGAC                                                                                          <  SRR4302543.7932828/94‑1 (MQ=60)
    CGCTCGATGAGCTGGGTGTAACGTGCTACGTCTTTACGTTTCAGGTAGTCGAGCAGTTTACGACGCTGAGAAACCATGCGCAGCAGACCGCGAC                                                                                          <  SRR4302543.7892440/94‑1 (MQ=60)
    CGCTCGATGAGCTGGGTGTAACGTGCTACGTCTTTACGTTTCAGGTAGTCGAGCAGTTTACGACGCTGAGAAACCATGCGCAGCAGACCGCGAC                                                                                          >  SRR4302543.3162025/1‑94 (MQ=60)
    CGCTCGATGAGCTGGGTGTAACGTGCTACGTCTTTACGTTTCAGGTAGTCCAGCAGTTTACGACGCTGAGAAACCATGCGCAGCAGACCGCGAC                                                                                          <  SRR4302543.6261493/94‑1 (MQ=60)
     GCTCGATGAGCTGGGTGTAACGTGCTACGTCTTTACGTTTCAGGTAGTCGAGCAGTTTACGACGCTGAGAAACCATGCGCAGCAGACCGCGACG                                                                                         <  SRR4302543.4577521/94‑1 (MQ=60)
     GCTCGATGAGCTGGGTGTAACGTGCTACGTCTTTACGTTTCAGGTAGTCGAGCAGTTTACGACGCTGAGAAACCATGCGCAGCAGACCGCGACG                                                                                         <  SRR4302543.278795/94‑1 (MQ=60)
     GCTCGATGAGCTGGGTGTAACGTGCTACGTCTTTACGTTTCAGGTAGTCGAGCAGTTTACGACGCTGAGAAACCATGCGCAGCAGACCGCGACG                                                                                         <  SRR4302543.3046981/94‑1 (MQ=60)
       TGGATGAGCTGGGTGTAACGTGCTACGTCTTTACGTTTCAGGTAGTCGAGCAGTTTACGACGCTGAGAAACCATGCGCAGCAGACCGCGACGGC                                                                                       <  SRR4302543.5685042/94‑1 (MQ=60)
       TCGATGAGCTGGGTGTAACGTGCTACGTCTTTACGTTTCAGGTAGTCGAGCAGTTTACGACGCTGAGAAACCATGCGCAGCAGACCGCGACGGC                                                                                       <  SRR4302543.856529/94‑1 (MQ=60)
       TCGATGAGCTGGGTGTAACGTGCTACGTCTTTACGTTTCAGGTAGTCGAGCAGTTTACGACGCTGAGAAACCATGCGCAGCAGACCGCGACGGC                                                                                       >  SRR4302543.7420811/1‑94 (MQ=60)
        CGATGAGCTGGGTGTAACGTGCTACGTCTTTACGTTTCAGGTAGTCGAGCAGTTTACGACGCTGAGAAACCATGCGCAGCAGACCGCGACGGCT                                                                                      >  SRR4302543.7794088/1‑94 (MQ=60)
        CGATGAGCTGGGTGTAACGTGCTACGTCTTTACGTTTCAGGTAGTCGAGCAGTTTACGACGCTGAGAAACCATGCGCAGCAGACCGCGACGGCT                                                                                      <  SRR4302543.7655692/94‑1 (MQ=60)
        CGATGAGCTGGGTGTAACGTGCTACGTCTTTACGTTTCAGGTAGTCGAGCAGTTTACGACGCTGAGAAACCATGCGCAGCAGACCGCGACGGCT                                                                                      <  SRR4302543.7940962/94‑1 (MQ=60)
        CGATGAGCTGGGTGTAACGTGCTACGTCTTTACGTTTCAGGTAGTCGAGCAGTTTACGACGCTGAGAAACCATGCGCAGCAGACCGCGACGGCT                                                                                      <  SRR4302543.6892749/94‑1 (MQ=60)
        CGATGAGCTGGGTGTAACGTGCTACGTCTTTACGTTTCAGGTAGTCGAGCAGTTTACGACGCTGAGAAACCATGCGCAGCAGACCGCGACGGCT                                                                                      <  SRR4302543.5231104/94‑1 (MQ=60)
        CGATGAGCTGGGTGTAACGTGCTACGTCTTTACGTTTCAGGTAGTCGAGCAGTTTACGACGCTGAGAAACCATGCGCAGCAGACCGCGACGGCT                                                                                      <  SRR4302543.7225432/94‑1 (MQ=60)
        CGATGAGCTGGGTGTAACGTGCTACGTCTTTACGTTTCAGGTAGTCGAGCAGTTTACGACGCTGAGAAACCATGCGCAGCAGACCGCGACGGCT                                                                                      >  SRR4302543.7391732/1‑94 (MQ=60)
        CGATGAGCTGGGTGTAACGTGCTACGTCTGTACGTTTCAGGTAGTCGAGCAGTTTACGACGCTGAGAAACCATGCGCAGCAGACCGCGACGGCT                                                                                      <  SRR4302543.4782583/94‑1 (MQ=60)
         GATGAGCTGGGTGTAACGTGCTACGTCTTTACGTTTCAGGTAGTCGAGCAGTTTACGACGCTGAGAAACCATGCGCAGCAGACCGCGACGGCTG                                                                                     <  SRR4302543.5974572/94‑1 (MQ=60)
         GATGAGCTGGGTGTAACGTGCTACGTCTTTACGTTTCAGGTAGTCGAGCAGTTTACGACGCTGAGAAACCATGCGCAGCAGACCGCGACGGCTG                                                                                     <  SRR4302543.945951/94‑1 (MQ=60)
          ATGAGCTGGGTGTAACGTGCTACGTCTTTACGTTTCAGGTAGTCGAGCAGTTTACGACGCTGAGCAACCATGCGCAGCAGACCGCGACGGCTGT                                                                                    >  SRR4302543.1352504/1‑94 (MQ=60)
          ATGAGCTGGGTGTAACGTGCTACGTCTTTACGTTTCAGGTAGTCGAGCAGTTTACGACGCTGAGAAACCATGCGCAGCAGACCGCGACGGCTGT                                                                                    >  SRR4302543.6316494/1‑94 (MQ=60)
          ATGAGCTGGGTGTAACGTGCTACGTCTTTACGTTTCAGGTAGTCGAGCAGTTTACGACGCTGAGAAACCATGCGCAGCAGACCGCGACGGCTGT                                                                                    >  SRR4302543.3434795/1‑94 (MQ=60)
          ATGAGCTGGGTGTAACGTGCTACGTCTTTACGTTTCAGGTAGTCGAGCAGTTTACGACGCTGAGAAACCATGCGCAGCAGACCGCGACGGCTGT                                                                                    >  SRR4302543.527409/1‑94 (MQ=60)
          ATGAGCTGGGTGTAACGTGCTACGTCTTTACGTTTCAGGTAGTCGAGCAGTTTACGACGCTGAGAAACCATGCGCAGCAGACCGCGACGGCTGT                                                                                    >  SRR4302543.1429376/1‑94 (MQ=60)
          ATGAGCTGGGTGTAACGTGCTACGCCTTTACGTTTCAGGTAGTCGAGCAGTTTACGACGCTGAGAAACCATGCGCAGCAGACCGCGACGGCTGT                                                                                    <  SRR4302543.2488421/94‑1 (MQ=60)
           TGAGCTGGGTGTAACGTGCTACGTCTTTACGTTTCAGGTAGTCGAGCAGTTTACGACGCTGAGAAACCATGCGCAGCAGACCGCGACGGCTGTG                                                                                   <  SRR4302543.7230900/94‑1 (MQ=60)
           TGAGCTGGGTGTAACGTGCTACGTCTTTACGTTTCAGGTAGTCGAGCAGTTTACGACGCTGAGAAACCATGCGCAGCAGACCGCGACGGCTGTG                                                                                   <  SRR4302543.7211375/94‑1 (MQ=60)
           TGAGCTGGGTGTAACGTGCTACGTCTTTACGTTTCAGGTAGTCGAGCAGTTTACGACGCTGAGAAACCATGCGCAGCAGACCGCGACGGCTGTG                                                                                   <  SRR4302543.2305824/94‑1 (MQ=60)
           TGAGCTGGGTGTAACGTGCTACGTCTTTACGTTTCAGGTAGTCGAGCAGTTTACGACGCTGAGAAACCATGCGCAGCAGACCGCGACGGCTGTG                                                                                   <  SRR4302543.7499941/94‑1 (MQ=60)
            GAGCTGGGTGTAACGTGCTACGTCTTTACGTTTCAGGTAGTCGAGCAGTTTACGACGCTGAGAAACCATGCGCAGCAGACCGCGACGGCTGTGG                                                                                  <  SRR4302543.4962673/94‑1 (MQ=60)
            GAGCTGGGTGTAACGTGCTACGTCTTTACGTTTCAGGTAGTCGAGCAGTTTACGACGCTGAGAAACCATGCGCAGCAGACCGCGACGGCTGTGG                                                                                  <  SRR4302543.7823842/94‑1 (MQ=60)
             AGCTGGGTGTAACGTGCTACGTCTTTACGTTTCAGGTAGTCGAGCAGTTTACGACGCTGAGAAACCATGCGCAGCAGACCGCGACGGCTGTGGT                                                                                 >  SRR4302543.2606924/1‑94 (MQ=60)
              GCTGGGTGTAACGTGCTACGTCTTTACGTTTCAGGTAGTCGAGCAGTTTACGACGCTGAGAAACCATGCGCAGCAGACCGCGACGGCTGTGGTG                                                                                <  SRR4302543.925235/94‑1 (MQ=60)
              GCTGGGTGTAACGTGCTACGTCTTTACGTTTCAGGTAGTCGAGCAGTTTACGACGCTGAGAAACCATGCGCAGCAGACCGCGACGGCTGTGGTG                                                                                >  SRR4302543.1069899/1‑94 (MQ=60)
               CTGGGTGTAACGTGCTACGTCTTTACGTTTCAGGTAGTCGAGCAGTTTACGACGCTGAGAAACCATGCGCAGCAGACCGCGACGGCTGTGGTGA                                                                               >  SRR4302543.3019646/1‑94 (MQ=60)
               CTGGGTGTAACGTGCTACGTCTTTACGTTTCAGGTAGTCGAGCAGTTTACGACGCTGAGAAACCATGCGCAGCAGACCGCGACGGCTGTGGTGA                                                                               <  SRR4302543.6437741/94‑1 (MQ=60)
               CTGGGTGTAACGTGCTACGTCTTTACGTTTCAGGTAGTCGAGCAGTTTACGACGCTGAGAAACCATGCGCAGCAGACCGCGACGGCTGTGGTGA                                                                               >  SRR4302543.5597067/1‑94 (MQ=60)
               CTGGGTGTAACGTGCTACGTCTTTACGTTTCAGGTAGTCGAGCAGTTTACGACGCTGAGAAACCATGCGCAGCAGACCGCGACGGCTGTGGTGA                                                                               >  SRR4302543.6503930/1‑94 (MQ=60)
                TGGGTGTAACGTGCTACGTCTTTACGTTTCAGGTAGTCGAGCAGTTTACGACGCTGAGAAACCATGCGCAGCAGACCGCGACGGCTGTGGTGAT                                                                              >  SRR4302543.3360370/1‑94 (MQ=60)
                TGGGTGTAACGTGCTACGTCTTTACGTTTCAGGTAGTCGAGCAGTTTACGACGCTGAGAAACCATGCGCAGCAGACCGCGACGGCTGTGGTGAT                                                                              <  SRR4302543.4899990/94‑1 (MQ=60)
                TGGGTGTAACGTGCTACGTCTTTACGTTTCAGGTAGTCGAGCAGTTTAAGACGCTGAGAAACCATGCGCAGCAGACCGCGACGGCTGTGGTGAT                                                                              <  SRR4302543.3533815/94‑1 (MQ=60)
                 GGGTGTAACGTGCTACGTCTTTACGTTTCAGGTAGTCGAGCAGTTTACGACGCTGAGAAACCATGCGCAGCAGACCGCGACGGCTGTGGTGATT                                                                             >  SRR4302543.267737/1‑94 (MQ=60)
                 GGGTGTAACGTGCTACGTCTTTACGTTTCAGGTAGTCGAGCAGTTTACGACGCTGAGAAACCATGCGCAGCAGACCGCGACGGCTGTGGTGATC                                                                             >  SRR4302543.1764464/1‑94 (MQ=60)
                 GGGTGTAACGTGCTACGTATTTACGTTTCAGGTAGTCGAGCAGTTTACGACGCTGAGAAACCATGCGCAGCAGACCGCGACGGCTGTGGTGATC                                                                             >  SRR4302543.1475228/1‑94 (MQ=60)
                  GGTGTAACGTGCTACGTCTTTACGTTTCAGGTAGTCGAGCAGTTTACGACGCTGAGAAACCATGCGCAGCAGACCGCGACGGCTGTTGTGATCT                                                                            >  SRR4302543.7346458/1‑94 (MQ=60)
                   GTGTAACGTGCTACGTCTTTACGTTTCAGGTAGTCGAGCAGTTTACGACGCTGAGAAACCATGCGCAGCAGACCGCGACGGCTGTGGTGATCTT                                                                           <  SRR4302543.1354825/94‑1 (MQ=60)
                   GTGTAACGTGCTACGTCTTTACGTTTCAGGTAGTCGAGCAGTTTACGACGCTGAGAAACCATGCGCAGCAGACCGCGACGGCTGTGGTGATCTT                                                                           <  SRR4302543.7607095/94‑1 (MQ=60)
                   GTGTAACGTGCTACGTCTTTACGTTTCAGGTAGTCGAGCAGTTTACGACGCTGAGAAACCATGCGCAGCAGACCGCGACGGCTGTGGTGATCTT                                                                           >  SRR4302543.6962803/1‑94 (MQ=60)
                    TGTAACGTGCTACGTCTTTACGTTTCAGGTAGTCGAGCAGTTTACGACGCTGAGAAACCATGCGCAGCAGACCGCGACGGCTGTGGTGATCTTT                                                                          <  SRR4302543.4951679/94‑1 (MQ=60)
                     GTAACGTGCTACGTCTTTACGTTTCAGGTAGTCGAGCAGTTTACGACGCTGAGAAACCATGCGCAGCAGACCGCGACGGCTGTGGTGATCTTTT                                                                         >  SRR4302543.1873125/1‑94 (MQ=60)
                      TAACGTGCTACGTCTTTACGTTTCAGGTAGTCGAGCAGTTTACGACGCTGAGAAACCATGCGCAGCAGACCGCGACGGCTGTGGTGATCTTTTT                                                                        >  SRR4302543.5158539/1‑94 (MQ=60)
                      TAACGTGCTACGTCTTTACGTTTCAGGTAGTCGAGCAGTTTACGACGCTGAGAAACCATGCGCAGCAGACCGCGACGGCTGTGGTGATCTTTTT                                                                        >  SRR4302543.1366470/1‑94 (MQ=60)
                      TAACGTGCTACGTCTTTACGTTTCAGGTAGTCGAGCAGTTTACGACGCTGAGAAACCATGCGCAGCAGACCGCGACGGCTGTGGTGATCTTTTT                                                                        >  SRR4302543.1366471/1‑94 (MQ=60)
                      TAACGTGCTACGTCTTTACGTTTCAGGTAGTCGAGCAGTTTACGACGCTGAGAAACCATGCGCAGCAGACCGCGACGGCTGTGGTGATCTTTTT                                                                        >  SRR4302543.2287423/1‑94 (MQ=60)
                       AACGTGCTACGTCTTTACGTTTCAGGTAGTCGAGCAGTTTACGACGCTGAGAAACCATGCGCAGCAGACCGCGACGGCTGTGGTGATCTTTTTT                                                                       >  SRR4302543.3800009/1‑94 (MQ=60)
                        ACGTGCTACGTCTTTACGTTTCAGGTAGTCGAGCAGTTTACGACGCTGAGAAACCATGCGCAGCAGACCGCGACGGCTGTGGTGATCTTTTTTG                                                                      <  SRR4302543.7230968/94‑1 (MQ=60)
                        ACGTGCTACGTCTTTACGTTTCAGGTAGTCGAGCAGTTTACGACGCTGAGAAACCATGCGCAGCAGACCGCGACGGCTGTGGTGATCTTTTTTG                                                                      >  SRR4302543.6920708/1‑94 (MQ=60)
                         CGTGCTACGTCTTTACGTTTCAGGTAGTCGAGCAGTTTACGACGCTGAGAAACCATGCGCAGCAGACCGCGACGGCTGTGGTGATCTTTTTTGT                                                                     >  SRR4302543.4083021/1‑94 (MQ=60)
                         CGTGCTACGTCTTTACGTTTCAGGTAGTCGAGCAGTTTACGACGCTGAGAAACCATGCGCAGCAGACCGCGACGGCTGTGGTGATCTTTTTTGT                                                                     >  SRR4302543.6966236/1‑94 (MQ=60)
                          GTGCTACGTCTTTACGTTTCAGGTAGTCGAGCAGTTTACGACGCTGAGAAACCATGCGCAGCAGACCGCGACGGCTGTGGTGATCTTTTTTGTG                                                                    >  SRR4302543.5733226/1‑94 (MQ=60)
                          GTGCTACGTCTTTACGTTTCAGGTAGTCGAGCAGTTTACGACGCTGAGAAACCATGCGCAGCAGACCGCGACGGCTGTGGTGATCTTTTTTGTG                                                                    <  SRR4302543.7515975/94‑1 (MQ=60)
                          GTGCTACGTCTTTACGTTTCAGGTAGTCGAGCAGTTTACGACGCTGAGAAACCATGCGCAGCAGACCGCGACGGCTGTGGTGATCTTTTTTGTG                                                                    <  SRR4302543.544775/94‑1 (MQ=60)
                          GTGCTACGTCTTTACGTTTCAGGTAGTCGAGCAGTTTACGACGCTGAGAAACCATGCGCAGCAGACCGCGACGGCTGTGGTGATCTTTTTTGTG                                                                    <  SRR4302543.3607535/94‑1 (MQ=60)
                          GTGCTACGTCTTTACGTTTCAGGTAGTCGAGCAGTTTACGACGCTGAGAAACCATGCGCAGCAGACCGCGACGGCTGTGGTGATCTTTTTTGTG                                                                    >  SRR4302543.4669421/1‑94 (MQ=60)
                           TGCTACGTCTTTACGTTTCAGGTAGTCGAGCAGTTTACGACGCTGAGAAACCATGCGCAGCAGACCGCGACGGCTGTGGTGATCTTTTTTGTat                                                                   >  SRR4302543.3909880/1‑92 (MQ=60)
                           TGCTACGTCTTTACGTTTCAGGTAGTCGAGCAGTTTACGACGCTGAGAAACCATGCGCAGCAGACCGCGACGGCTGTGGTGATCTTTTTTGTGC                                                                   <  SRR4302543.4856445/94‑1 (MQ=60)
                            GCTACGTCTTTACGTTTCAGGTAGTCGAGCAGTTTACGACGCTGAGAAACCATGCGCAGCAGACCGCGACGGCTGTGGTGATCTTTTTTGTGCT                                                                  >  SRR4302543.5449989/1‑94 (MQ=60)
                            GCTACGTCTTTACGTTTCAGGTAGTCGAGCAGTTTACGACGCTGAGAAACCATGCGCAGCAGACCGCGACGGCTGTGGTGATCTTTTTTGTGCT                                                                  >  SRR4302543.4896297/1‑94 (MQ=60)
                             CTACGTCTTTACGTTTCAGGTAGTCGAGCAGTTTACGACGCTGAGAAACCATGCGCAGCAGACCGCGACGGCTGTGGTGATCTTTTTTGTGCTC                                                                 >  SRR4302543.631235/1‑94 (MQ=60)
                             CTACGTCTTTACGTTTCAGGTAGTCGAGCAGTTTACGACGCTGAGAAACCATGCGACGCAGAACGCGACGGCTGTGGTGATCTTTTTTGTGCTT                                                                 >  SRR4302543.3320951/1‑94 (MQ=60)
                              TACGTCTTTACGTTTCAGGTAGTCGAGCAGTTTACGACGCTGAGAAACCATGCGCAGCAGACCGCGACGGCTGTGGTGATCTTTTTTGTGCTCT                                                                <  SRR4302543.1104049/94‑1 (MQ=60)
                              TACGTCTTTACGTTTCAGGTAGTCGAGCAGTTTACGACGCTGAGAAACCATGCGCAGCAGACCGCGACGGCTGTGGTGATCTTTTTTGTGCTCT                                                                <  SRR4302543.3894578/94‑1 (MQ=60)
                              TACGTCTTTACGTTTCAGGTAGTCGAGCAGTTTACGACGCTGAGAAACCATGCGCAGCAGACCGCGACGGCTGTGGTGATCTTTTTTGTGCTCT                                                                <  SRR4302543.1119522/94‑1 (MQ=60)
                              TACGTCTTTACGTTTCAGGTAGTCGAGCAGTTTACGACGCTGAGAAACCATGCGCAGCAGACCGCGACGGCTGTGGTGATCTTTTTTGTGCTCT                                                                >  SRR4302543.7660214/1‑94 (MQ=60)
                                CGTCTTTACGTTTCAGGTAGTCGAGCAGTTTACGACGCTGAGAAACCATGCGCAGCAGACCGCGACGGCTGTGGTGATCTTTTTTGTGCTCTGC                                                              >  SRR4302543.6897166/1‑94 (MQ=60)
                                CGTCTTTACGTTTCAGGTAGTCGAGCAGTTTACGACGCTGAGAAACCATGCGCAGCAGACCGCGACGGCTGTGGTGATCTTTTTTGTGCTCTGC                                                              <  SRR4302543.7287781/94‑1 (MQ=60)
                                CGTCTTTACGTTTCAGGTAGTCGAGCAGTTTACGACGCTGAGAAACCATGCGCAGCAGACCGCGACGGCTGTGGTGATCTTTTTTGTGCTCTGC                                                              >  SRR4302543.1994586/1‑94 (MQ=60)
                                CGTCTTTACGTTTCAGGTAGTCGAGCAGTTTACGACGCTGAGAAACCATGCGCAGCAGACCGCGACGGCTGTGGTGATCTTTTTTGTGCTCTGC                                                              >  SRR4302543.5605880/1‑94 (MQ=60)
                                 GTCTTTACGTTTCAGGTAGTCGAGCAGTTTACGACGCTGAGAAACCATGCGCAGCAGACCGCGACGGCTGTGGTGATCTTTTTTGTGCTCTGCA                                                             >  SRR4302543.1545595/1‑94 (MQ=60)
                                 GTCTTTACGTTTCAGGTAGTCGAGCAGTTTACGACGCTGAGAAACCATGCGCAGCAGACCGCGACGGCTGTGGTGATCTTTTTTGTGCTCTGCA                                                             >  SRR4302543.3937665/1‑94 (MQ=60)
                                 GTCTTTACGTTTCAGGTAGTCGAGCAGTTTACGACGCTGAGAAACCATGCGCAGCAGACCGCGACGGCTGTGGTGATCTTTTTTGTGCTCTGCA                                                             <  SRR4302543.6982912/94‑1 (MQ=60)
                                   CTTTACGTTTCAGGTAGTCGAGCAGTTTACGACGCTGAGAAACCATGCGCAGCAGACCGCGACGGCTGTGGTGATCTTTTTTGTGCTCTGCAAA                                                           >  SRR4302543.4746218/1‑94 (MQ=60)
                                    TTTACGTTTCAGGTAGTCGAGCAGTTTACGACGCTGATAAACCATGCGCAGCAGACCGCGACGGCTGTGGTGATCTTTTTTGTGCTCTGCAAAG                                                          <  SRR4302543.740514/94‑1 (MQ=60)
                                    TTTACGTTTCAGGTAGTCGAGCAGTTTACGACGCTGAGAAACCATGCGCAGCAGACCGCGACGGCTGTGGTGATCTTTTTTGTGCTCTGCAAAG                                                          <  SRR4302543.3847858/94‑1 (MQ=60)
                                    TTTACGTTTCAGGTAGTCGAGCAGTTTACGACGCTGAGAAACCATGCGCAGCAGACCGCGACGGCTGTGGTGATCTTTTTTGTGCTCTGCAAAG                                                          <  SRR4302543.43892/94‑1 (MQ=60)
                                    TTTACGTTTCAGGTAGTCGAGCAGTTTACGACGCTGAGAAACCATGCGCAGCAGACCGCGACGGCTGTGGTGATCTTTTTTGTGCTCTGCAAAG                                                          >  SRR4302543.3704268/1‑94 (MQ=60)
                                    TTTACGTTTCAGGTAGTCGAGCAGTTTACGACGCTGAGAAACCATGCGCAGCAGACCGCGACGGCTGTGGTGATCTTTTTTGTGCTCTGCAAAG                                                          >  SRR4302543.3635656/1‑94 (MQ=60)
                                    TTTACGTTTCAGGTAGTCGAGCAGTTTACGACGCTGAGAAACCATGCGCAGCAGACCGCGACGGCTGTGGTGATCTTTTTTGTGCTCTGCAAAG                                                          <  SRR4302543.2941047/94‑1 (MQ=60)
                                     TTACGTTTCAGGTAGTCGAGCAGTTTACGACGCTGAGAAACCATGCGCAGCAGACCGCGACGGCTGTGGTGATCTTTTTTGTGCTCTGCAAAGT                                                         <  SRR4302543.2264709/94‑1 (MQ=60)
                                     TTACGTTTCAGGTAGTCGAGCAGTTTACGACGCTGAGAAACCATGCGCAGCAGACCGCGACGGCTGTGGTGATCTTTTTTGTGCTCTGCAAAGT                                                         <  SRR4302543.589350/94‑1 (MQ=60)
                                      TACGTTTCAGGTAGTCGAGCAGTTTACGACGCTGAGAAACCATGCGCAGCAGACCGCGACGGCTGTGGTGATCTTTTTTGTGCTCTGCAAAGTG                                                        <  SRR4302543.4310211/94‑1 (MQ=60)
                                      TACGTTTCAGGTAGTCGAGCAGTTTACGACGCTGAGAAACCATGCGCAGCAGACCGCGACGGCTGTGGTGATCTTTTTTGTGCTCTGCAAAGTG                                                        >  SRR4302543.3096083/1‑94 (MQ=60)
                                       ACGTTTCAGGTAGTCGAGCAGTTTACGACGCTGAGAAACCATGCGCAGCAGACCGCGACGGCTGTGGTGATCTTTTTTGTGCTCTGCAAAGTGG                                                       <  SRR4302543.6497309/94‑1 (MQ=60)
                                        CGTTTCAGGTAGTCGAGCAGTTTACGACGCTGAGAAACCATGCGCAGCAGACCGCGACGGCTGTGGTGATCTTTTTTGTGCTCTGCAAAGTGGC                                                      >  SRR4302543.4199917/1‑94 (MQ=60)
                                        CGTTTCAGGTAGTCGAGCAGTTTACGACGCTGAGAAACCATGCGCAGCAGACCGCGACGGCTGTGGTGATCTTTTTTGTGCTCTGCAAAGTGGC                                                      <  SRR4302543.1101492/94‑1 (MQ=60)
                                         GTTTCAGGTAGTCGAGCAGTTTACGACGCTGAGAAACCATGCGCAGCAGACCGCGACGGCTGTGGTGATCTTTTTTGTGCTCTGCAAAGTGGCC                                                     <  SRR4302543.5475540/94‑1 (MQ=60)
                                         GTTTCAGGTAGTCGAGCAGTTTACGACGCTGAGAAACCATGCGCAGCAGACCGCGACGGCTGTGGTGATCTTTTTTGTGCTCTGCAAAGTGGCC                                                     >  SRR4302543.7990570/1‑94 (MQ=60)
                                         GTTTCAGGTAGTCGAGCAGTTTACGACGCTGAGAAACCATGCGCAGCAGACCGCGACGGCTGTGGTGATCTTTTTTGTGCTCTGCAAAGTGGAC                                                     >  SRR4302543.6876451/1‑94 (MQ=60)
                                          TTTCAGGTAGTCGAGCAGTTTACGACGCTGAGAAACCATGCGCAGCAGACCGCGACGGCTGTGGTGATCTTTTTTGTGCTCTGCAAAGTGGCCC                                                    <  SRR4302543.6463773/94‑1 (MQ=60)
                                           TTCAGGTAGTCGAGCAGTTTACGACGCTGAGAAACCATGCGCAGCAGACCGCGACGGCTGTGGTGATCTTTTTTGTGCTCTGCAAAGTGGCCCT                                                   >  SRR4302543.5979958/1‑94 (MQ=60)
                                           TTCAGGTAGTCGAGCAGTTTACGACGCTGAGAAACCATGCGCAGCAGACCGCGACGGCTGTGGTGATCTTTTTTGTGCTCTGCAAAGTGGCCCT                                                   <  SRR4302543.6399501/94‑1 (MQ=60)
                                            TCAGGTAGTCGAGCAGTTTACGACGCTGAGAAACCATGCGCAGCAGACCGCGACGGCTGTGGTGATCTTTTTTGTGCTCTGCAAAGTGGCCCTG                                                  <  SRR4302543.7367429/94‑1 (MQ=60)
                                            TCAGGTAGTCGAGCAGTTTACGACGCTGAGAAACCATGCGCAGCAGACCGCGACGGCTGTGGTGATCTTTTTTGTGCTCTGCAAAGTGGCCCTG                                                  <  SRR4302543.6403206/94‑1 (MQ=60)
                                            TCAGGTAGTCGAGCAGTTTACGACGCTGAGAAACCATGCGCAGCAGACCGCGACGGCTGTGGTGATCTTTTTTGTGCTCTGCAAAGTGGCCCTG                                                  <  SRR4302543.794300/94‑1 (MQ=60)
                                             CAGGTAGTCGAGCAGTTTACGACGCTGAGAAACCATGCGCAGCAGACCGCGACGGCTGTGGTGATCTTTTTTGTGCTCTGCAAAGTGGCCCTGC                                                 >  SRR4302543.846558/1‑94 (MQ=60)
                                             CAGGTAGTCGAGCAGTTTACGACGCTGAGAAACCATGCGCAGCAGACCGCGACGGCTGTGGTGATCTTTTTTGTGCTCTGCAAAGTGGCCCTGC                                                 >  SRR4302543.356961/1‑94 (MQ=60)
                                              AGGTAGTCGAGCAGTTTACGACGCTGAGAAACCATGCGCAGCAGACCGCGACGGCTGTGGTGATCTTTTTTGTGCTCTGCAAAGTGGCCCTGCA                                                >  SRR4302543.3941031/1‑94 (MQ=60)
                                              AGGTAGTCGAGCAGTTTACGACGCTGAGAAACCATGCGCAGCAGACCGCGACGGCTGTGGTGATCTTTTTTGTGCTCTGCAAAGTGGCCCTGCA                                                >  SRR4302543.7824157/1‑94 (MQ=60)
                                              AGGTAGTCGAGCAGTTTACGACGCTGAGAAACCATGCGCAGCAGACCGCGACGGCTGTGGTGATCTTTTTTGTGCTCTGCAAAGTGGCCCTGCA                                                >  SRR4302543.4811905/1‑94 (MQ=60)
                                               GGTAGTCGAGCAGTTTACGACGCTGAGAAACCATGCGCAGCAGACCGCGACGGCTGTGGTGATCTTTTTTGTGCTCTGCAAAGTGGCCCTGCAG                                               <  SRR4302543.5046754/94‑1 (MQ=60)
                                               GGTAGTCGAGCAGTTTACGACGCTGAGAAACCATGCGCAGCAGACCGCGACGGCTGTGGTGATCTTTTTTGTGCTCTGCAAAGTGGCCCTGCAG                                               <  SRR4302543.7260318/94‑1 (MQ=60)
                                               GGTAGTCGAGCAGTTTACGACGCTGAGAAACCATGCGCAGCAGACCGCGACGGCTGTGGTGATCTTTTTTGTGCTCTGCAAAGTGGCCCTGCAG                                               <  SRR4302543.6310577/94‑1 (MQ=60)
                                                GTAGTCGAGCAGTTTACGACGCTGAGAAACCATGCGCAGCAGACCGCGACGGCTGTGGTGATCTTTTTTGTGCTCTGCAAAGTGGCCCTGCAGG                                              <  SRR4302543.6122269/94‑1 (MQ=60)
                                                  AGTCGAGCAGTTTACGACGCTGAGAAACCATGCGCAGCAGACCGCGACGGCTGTGGTGATCTTTTTTGTGCTCTGCAAAGTGGCCCTGCAGGTG                                            >  SRR4302543.1076126/1‑94 (MQ=60)
                                                  AGTCGAGCAGTTTACGACGCTGAGAAACCATGCGCAGCAGACCGCGACGGCTGTGGTGATCTTTTTTGTGCTCTGCAAAGTGGCCCTGCAGGTG                                            <  SRR4302543.404895/94‑1 (MQ=60)
                                                  AGTCGAGCAGTTTACGACGCTGAGAAACCATGCGCAGCAGACCGCGACGGCTGTGGTGATCTTTTTTGTGCTCTGCAAAGTGGCCCTGCAGGTG                                            <  SRR4302543.5907593/94‑1 (MQ=60)
                                                    TCGAGCAGTTTACGACGCTGAGAAACCATGCGCAGCAGACCGCGACGGCTGTGGTGATCTTTTTTGTGCTCTGCAAAGTGGCCCTGCAGGTGGT                                          >  SRR4302543.2987819/1‑94 (MQ=60)
                                                     CGAGCAGTTTACGACGCTGAGAAACCATGCGCAGCAGACCGCGACGGCTGTGGTGATCTTTTTTGTGCTCTGCAAAGTGGCCCTGCAGGTGGTT                                         <  SRR4302543.2695571/94‑1 (MQ=60)
                                                     CGAGCAGTTTACGACGCTGAGAAACCATGCGCAGCAGACCGCGACGGCTGTGGTGATCTTTTTTGTGCTCTGCAAAGTGGCCCTGCAGGTGGTT                                         >  SRR4302543.6115305/1‑94 (MQ=60)
                                                     CGAGCAGTTTACGACGCTGAGAAACCATGCGCAGCAGACCGCGACGGCTGTGGTGATATTTTTTGTGCTCTGCAAAGTGGCCCTGCAGGTGGTT                                         <  SRR4302543.1800624/94‑1 (MQ=60)
                                                      GAGCAGTTTACGACGCTGAGAAACCATGCGCAGCAGACCGCGACGGCTGTGGTGATCTTTTTTGTGCTCTGCAAAGTGGCCCTGCAGGTGGTTG                                        >  SRR4302543.2771232/1‑94 (MQ=60)
                                                      GAGCAGTTTACGACGCTGAGAAACCATGCGCAGCAGACCGCGACGGCTGTGGTGATCTTTTTTGTGCTCTGCAAAGTGGCCCTGCAGGTGGTTG                                        <  SRR4302543.5174709/94‑1 (MQ=60)
                                                       AGCAGTTTACGACGCTGAGAAACCATGCGCAGCAGACCGCGACGGCTGTGGTGATCTTTTTTGTGCTCTGCAAAGTGGCCCTGCAGGTGGTTGA                                       >  SRR4302543.1249965/1‑94 (MQ=60)
                                                         CAGTTTACGACGCTGAGAAACCATGCGCAGCAGACCGCGACGGCTGTGGTGATCTTTTTTGTGCTCTGCAAAGTGGCCCTGCAGGTGGTTGATC                                     <  SRR4302543.5935508/94‑1 (MQ=60)
                                                          AGTTTACGACGCTGAGAAACCATGCGCAGCAGACCGCGACGGCTGTGGTGATCTTTTTTGTGCTCTGCAAAGTGGCCCTGCAGGTGGTTGATCT                                    >  SRR4302543.2841534/1‑94 (MQ=60)
                                                          AGTTTACGACGCTGAGAAACCATGCGCAGCAGACCGCGACGGCTGTGGTGATCTTTTTTGTGCTCTGCAAAGTGGCCCTGCAGGTGGTTGATCT                                    <  SRR4302543.2480422/94‑1 (MQ=60)
                                                          AGTTTACGACGCTGAGAAACCATGCGCAGCAGACCGCGACGGCTGTGGTGATCTTTTTTGTGCTCTGCAAAGTGGCCCTGCAGGTGGTTGATCT                                    <  SRR4302543.6505887/94‑1 (MQ=60)
                                                          AGTTTACGACGCTGAGAAACCATGCGCAGCAGACCGCGACGGCTGTGGTGATCTTTTTTGTGCTCTGCAAAGTGGCCCTGCAGGTGGTTGATCT                                    <  SRR4302543.5343187/94‑1 (MQ=60)
                                                          AGTTTACGACGCTGAGAAACCATGCGCAGCAGACCGCGACGGCGGTGGTGATCTTTTTTGTGCTCTGCAAAGTGGCCCTGCAGGTGGTTGATCT                                    >  SRR4302543.4126322/1‑94 (MQ=60)
                                                           GTTTACGACGCTGAGAAACCATGCGCAGCAGACCGCGACGGCTGTGGTGATCTTTTTTGTGCTCTGCAAAGTGGCCCTGCAGGTGGTTGATCTG                                   >  SRR4302543.4318014/1‑94 (MQ=60)
                                                           GTTTACGACGCTGAGAAACCATGCGCAGCAGACCGCGACGGCTGTGGTGATCTTTTTTGTGCTCTGCAAAGTGGCCCTGCAGGTGGTTGATCTG                                   >  SRR4302543.1318003/1‑94 (MQ=60)
                                                           GTTTACGACGCTGAGAAACCATGCGCAGCAGACCGCGACGGCTGTGGTGATCTTTTTTGTGCTCTGCAAAGTGGCCCTGCAGGTGGTTGATCTG                                   >  SRR4302543.2364647/1‑94 (MQ=60)
                                                           GTTTACGACGCTGAGAAACCATGCGCAGCAGACCGCGACGGCTGTGGTGATCTTTTTTGTGCTCTGCAAAGTGGCCCTGCAGGTGGTTGATCTG                                   <  SRR4302543.3027866/94‑1 (MQ=60)
                                                           GTTTACGACGCTGAGAAACCATGCGCAGCAGACCGCGACGGCTGTGGTGATCTTTTTTGTGCTCTGCAAAGTGGCCCTGCAGGTGGTTGATCTG                                   <  SRR4302543.3027860/94‑1 (MQ=60)
                                                           GTTTACGACGCTGAGAAACCATGCGCAGCAGACCGCGACGGCTGTGGTGATCTTTTTTGTGCTCTGCAAAGTGGCCCTGCAGGTGGTTGATCTG                                   >  SRR4302543.2034758/1‑94 (MQ=60)
                                                           GTTTACGACGCTGAGAAACCATGCGCAGCAGACCGCGACGGCTGTGGTGATCTTTTTTGTGCTCTGCAAAGTGGCCCTGCAGGTGGTTGATCTG                                   >  SRR4302543.3132505/1‑94 (MQ=60)
                                                           GTTTACGACGCTGAGAAACCATGCGCAGCAGACCGCGACGGCTGTGGTGATCTTTTTTGTGCTCTGCAAAGTGGCCCTGCAGGTGGTTGATCTG                                   >  SRR4302543.61262/1‑94 (MQ=60)
                                                            TTTACGACGCTGAGAAACCATGCGCAGCAGACCGCGACGGCTGTGGTGATCTTTTTTGTGCTCTGCAAAGTGGCCCTGCAGGTGGTTGATCTGT                                  >  SRR4302543.4305015/1‑94 (MQ=60)
                                                             TTACGACGCTGAGAAACCATGCGCAGCAGACCGCGACGGCTGTGGTGATCTTTTTTGTGCTCTGCAAAGTGGCCCTGCAGGTGGTTGATCTGTG                                 <  SRR4302543.3051278/94‑1 (MQ=60)
                                                               ACGACGCTGAGAAACCATGCGCAGCAGACCGCGACGGCTGTGGTGATCTTTTTTGTGCTCTGCAAAGTGGCCCTGCAGGTGGTTGATCTGTGCA                               <  SRR4302543.2901175/94‑1 (MQ=60)
                                                               ACGACGCTGAGAAACCATGCGCAGCAGACCGCGACGGCTGTGGTGATCTTTTTTGTGCTCTGCAAAGTGGCCCTGCAGGTGGTTGATCTGTGCA                               <  SRR4302543.2426881/94‑1 (MQ=60)
                                                               ACGACGCTGAGAAACCATGCGCAGCAGACCGCGACGGCTGTGGTGATCTTTTTTGTGCTCTGCAAAGTGGCCCTGCAGGTGGTTGATCTGTGCA                               >  SRR4302543.2524224/1‑94 (MQ=60)
                                                                CGACGCTGAGAAACCATGCGCAGCAGACCGCGACGGCTGTGGTGATCTTTTTTGTGCTCTGCAAAGTGGCCCTGCAGGTGGTTGATCTGTGCAG                              <  SRR4302543.5747018/94‑1 (MQ=60)
                                                                CGACGCTGAGAAACCATGCGCAGCAGACCGCGACGGCTGTGGTGATCTTTTTTGTGCTCTGCAAAGTGGCCCTGCAGGTGGTTGATCTGTGCAG                              >  SRR4302543.4280003/1‑94 (MQ=60)
                                                                CGACGCTGAGAAACCATGCGCAGCAGACCGCGACGGCTGTGGTGATCTTTTTTGTGCTCTGCAAAGTGGCCCTGCAGGTGGTTGATCTGTGCAG                              >  SRR4302543.3288373/1‑94 (MQ=60)
                                                                 GACGCTGAGAAACCATGCGCAGCAGACCGCGACGGCTGTGGTGATCTTTTTTGTGCTCTGCAAAGTGGCCCTGCAGGTGGTTGATCTGTGCAGT                             <  SRR4302543.5132782/94‑1 (MQ=60)
                                                                  ACGCTGAGAAACCATGCGCAGCAGACCGCGACGGCTGTGGTGATCTTTTTTGTGCTCTGCAAAGTGGCCCTGCAGGTGGTTGATCTGTGCAGTC                            <  SRR4302543.4700803/94‑1 (MQ=60)
                                                                   CGCTGAGAAACCATGCGCAGCAGACCGCGACGGCTGTGGTGATCTTTTTTGTGCTCTGCAAAGTGGCCCTGCAGGTGGTTGATCTGTGCAGTCA                           <  SRR4302543.7016550/94‑1 (MQ=60)
                                                                   CGCTGAGAAACCATGCGCAGCAGACCGCGACGGCTGTGGTGATCTTTTTTGTGCTCTGCAAAGTGGCCCTGCAGGTGGTTGATCTGTGCAGTCA                           >  SRR4302543.6266116/1‑94 (MQ=60)
                                                                    GCTGAGAAACCATGCGCAGCAGACCGCGACGGCTGTGGTGATCTTTTTTGTGCTCTGCAAAGTGGCCCTGCAGGTGGTTGATCTGTGCAGTCAG                          <  SRR4302543.7932367/94‑1 (MQ=60)
                                                                    GCTGAGAAACCATGCGCAGCAGACCGCGACGGCTGTGGTGATCTTTTTTGTGCTCTGCAAAGTGGCCCTGCAGGTGGTTGATCTGTGCAGTCAG                          <  SRR4302543.1181740/94‑1 (MQ=60)
                                                                    GCTGAGAAACCATGCGCAGCAGACCGCGACGGCTGTGGTGATCTTTTTTGTGCTCTGCAAAGTGGCCCTGCAGGGGGTTGATCTGTGCAGTCAG                          >  SRR4302543.1442548/1‑94 (MQ=60)
                                                                     CTGTGAAACCATGCGCAGCAGACCGCGACGGCTGTGGTGATCTTTTTTGTGCTCTGCAAAGTGGCCCTGCAGGTGGTTGATCTGTGCAGTCAGC                         >  SRR4302543.39249/1‑94 (MQ=60)
                                                                     CTGAGAAACCATGCGCAGCAGACCGCGACGGCTGTGGTGATCTTTTTTGTGCTCTGCAAAGTGGCCCTGCAGGTGGTTGATCTGTGCAGTCAGC                         >  SRR4302543.4321132/1‑94 (MQ=60)
                                                                     CTGAGAAACCATGCGCAGCAGACCGCGACGGCTGTGGTGATCTTTTTTGTGCTCTGCAAAGTGGCCCTGCAGGTGGTTGATCTGTGCAGTCAGC                         >  SRR4302543.1442683/1‑94 (MQ=60)
                                                                     CTGAGAAACCATGCGCAGCAGACCGCGACGGCTGTGGTGATCTTTTTTGTGCTCTGCAAAGTGGCCCTGCAGGTGGTTGATCTGTGCAGTCAGC                         <  SRR4302543.3282407/94‑1 (MQ=60)
                                                                      TGAGAAACCATGCGCAGCAGACCGCGACGGCTGTGGTGATCTTTTTTGTGCTCTGCAAAGTGGCCCTGCAGGTGGTTGATCTGTGCAGTCAGCA                        >  SRR4302543.4359862/1‑94 (MQ=60)
                                                                       GAGAAACCATGCGCAGCAGACCGCGACGGCTGTGGTGATCTTTTTTGTGCTCTGCAAAGTGGCCCTGCAGGTGGTTGATCTGTGCAGTCAGCAG                       <  SRR4302543.1186458/94‑1 (MQ=60)
                                                                       GAGAAACCATGCGCAGCAGACCGCGACGGCTGTGGTGATCTTTTTTGTGCTCTGCAAAGTGGCCCTGCAGGTGGTTGATCTGTGCAGTCAGCAG                       <  SRR4302543.7794207/94‑1 (MQ=60)
                                                                       GAGAAACCATGCGCAGCAGACCGCGACGGCTGTGGTGATCTTTTTTGTGCTCTGCAAAGTGGCCCTGCAGGTGGTTGATCTGTGCAGTCAGCAG                       <  SRR4302543.4043642/94‑1 (MQ=60)
                                                                       GAGAAACCATGCGCAGCAGACCGCGACGGCTGTGGTGATCTTTTTTGTGCTCTGCAAAGTGGCCCTGCAGGTGGTTGATCTGTGCAGTCAGCAG                       <  SRR4302543.7726340/94‑1 (MQ=60)
                                                                       GAGAAACCATGCGCAGCAGACCACGACGGCTGTGGTGATCTTTTTTGTGCTCTGCAAAGTGGCCCTGCAGGTGGTTGATCTGTGCAGTCAGCAG                       >  SRR4302543.5241054/1‑94 (MQ=60)
                                                                        AGAAACCATGCGCAGCAGACCGCGACGGCTGTGGTGATCTTTTTTGTGCTCTGCAAAGTGGCCCTGCAGGTGGTTGATCTGTGCAGTCAGCAGT                      >  SRR4302543.3188704/1‑94 (MQ=60)
                                                                        AGAAACCATGCGCAGCAGACCGCGACGGCTGTGGTGATCTTTTTTGTGCTCTGCAAAGTGGCCCTGCAGGTGGTTGATCTGTGCAGTCAGCAGT                      <  SRR4302543.1243227/94‑1 (MQ=60)
                                                                        AGAAACCATGCGCAGCAGACCGCGACGGCTGTGGTGATCTTTTTTGTGCTCTGCAAAGTGGCCCTGCAGGTGGTTGATCTGTGCAGTCAGCAGT                      <  SRR4302543.7422815/94‑1 (MQ=60)
                                                                        AGAAACCATGCGCAGCAGACCGCGACGGCTGTGGTGATCTTTTTTGTGCTCTGCAAAGTGGCCCTGCAGGTGGTTGATCTGTGCAGTCAGCAGT                      <  SRR4302543.1403315/94‑1 (MQ=60)
                                                                         GAAACCATGCGCAGCAGACCGCGACGGCTGTGGTGATCTTTTTTGTGCTCTGCAAAGTGGCCCTGCAGGTGGTTGATCTGTGCAGTCAGCAGTG                     >  SRR4302543.5737412/1‑94 (MQ=60)
                                                                         GAAACCATGCGCAGCAGACCGCGACGGCTGTGGTGATCTTTTTTGTGCTCTGCAAAGTGGCCCTGCAGGTGGTTGATCTGTGCAGTCAGCAGTG                     <  SRR4302543.1198578/94‑1 (MQ=60)
                                                                         GAAACCATGCGCAGCAGACCGCGACGGCTGTGGTGATCTTTTTTGTGCTCTGCAAAGTGGCCCTGCAGGTGGTTGATCTGTGCAGTCAGCAGTG                     <  SRR4302543.480859/94‑1 (MQ=60)
                                                                           AACCATGCGCAGCAGACCGCGACGGCTGTGGTGATCTTTTTTGTGCTCTGCAAAGTGGCCCTGCAGGTGGTTGATCTGTGCAGTCAGCAGTGCT                   >  SRR4302543.3768738/1‑94 (MQ=60)
                                                                           AACCATGCGCAGCAGACCGCGACGGCTGTGGTGATCTTTTTTGTGCTCTGCAAAGTGGCCCTGCAGGTGGTTGATCTGTGCAGTCAGCAGTGCT                   >  SRR4302543.7972646/1‑94 (MQ=60)
                                                                           AACCATGCGCAGCAGACCGCGACGGCTGTGGTGATCTTTTTTGTGCTCTGCAAAGTGGCCCTGCAGGTGGTTGATCTGTGCAGTCAGCAGTGCT                   >  SRR4302543.5684791/1‑94 (MQ=60)
                                                                            ACCATGCGCAGCAGACCGCGACGGCTGTGGTGATCTTTTTTGTGCTCTGCAAAGTGGCCCTGCAGGTGGTTGATCTGTGCAGTCAGCAGTGCTA                  >  SRR4302543.2243415/1‑94 (MQ=60)
                                                                             CCATGCGCAGCAGACCGCGACGGCTGTGGTGATCTTTTTTGTGCTCTGCAAAGTGGCCCTGCAGGTGGTTGATCTGTGCAGTCAGCAGTGCTAC                 <  SRR4302543.7384487/94‑1 (MQ=60)
                                                                              CATGCGCAGCAGTCCGCGACGGCTGTGGTGATCTTTTTTGTGCTCTGCAAAGTGGCCCTGCAGGTGGTTGATCTGTGCAGTCAGCAGTGCTACC                <  SRR4302543.1413822/94‑1 (MQ=60)
                                                                              CATGCGCAGCAGACCGCGACGGCTGTGGTGATCTTTTTTGTGCTCTGCAAAGTGGCCCTGCAGGTGGTTGATCTGTGCAGTCAGCAGTGCTACC                >  SRR4302543.1489673/1‑94 (MQ=60)
                                                                              CATGCGCAGCAGACCGCGACGGCTGTGGTGATCTTTTTTGTGCTCTGCAAAGTGGCCCTGCAGGTGGTTGATCTGTGCAGTCAGCAGTGCTACC                <  SRR4302543.7460860/94‑1 (MQ=60)
                                                                               ATGCGCAGCAGACCGCGACGGCTGTGGTGATCTTTTTTGTGCTCTGCAAAGTGGCCCTGCAGGTGGTTGATCTGTGCAGTCAGCAGTGCTACCT               >  SRR4302543.4660770/1‑94 (MQ=60)
                                                                                 GCGCAGCAGACCGCGACTGCTGTGGTGATCTTTTGTGTGCTCTGCAAAGTGGCCCTGCAGGTGGTTGATCTGTGCAGTCAGCAGTGCTACCTGA             <  SRR4302543.1726207/94‑1 (MQ=60)
                                                                                  CGCAGCAGACCGCGACGGCTGTGGTGATCTTTTTTGTGCTCTGCAAAGTGGCCCTGCAGGTGGTTGATCTGTGCAGTCAGCAGTGCTACCTGAA            <  SRR4302543.1494488/94‑1 (MQ=60)
                                                                                      GCAGACCGCGACGGCTGTGGTGATCTTTTTTGTGCTCTGCAAAGTGGCCCTGCAGGTGGTTGATCTGTGCAGTCAGCAGTGCTACCTGAACTTC        >  SRR4302543.3134399/1‑94 (MQ=60)
                                                                                      GCAGACCGCGACGGCTGTGGTGATCTTTTTTGTGCTCTGCAAAGTGGCCCTGCAGGTGGTTGATCTGTGCAGTCAGCAGTGCTACCTGAACTTC        <  SRR4302543.6142108/94‑1 (MQ=60)
                                                                                       CAGACCGCGACGGCTGTGGTGATCTTTTTTGTGCTCTGCAAAGTGGCCCTGCAGGTGGTTGATCTGTGCAGTCAGCAGTGCTACCTGAACTTCG       <  SRR4302543.32/94‑1 (MQ=60)
                                                                                        AGACCGCGACGGCTGTGGTGATCTTTTTTGTGCTCTGCAAAGTGGCCCTGCAGGTGGTTGATCTGTGCAGTCAGCAGTGCTACCTGAACTTCGG      <  SRR4302543.3865207/94‑1 (MQ=60)
                                                                                        AGACCGCGACGGCTGTGGTGATCTTTTTTGTGCTCTGCAAAGTGGCCCTGCAGGTGGTTGATCTGTGCAGTCAGCAGTGCTACCTGAACTTCGG      >  SRR4302543.238487/1‑94 (MQ=60)
                                                                                        AGACCGCGACGGCTGTGGTGATCTTTTTTGTGCTCTGCAAAGTGGCCCTGCAGGTGGTTGAGCTGTGCAGTCAGCAGTGCTACCTGAACTTCGG      >  SRR4302543.7126722/1‑94 (MQ=60)
                                                                                         GACCGCGACGGCTGTGGTGATCTTTTTTGTGCTCTGCAAAGTGGCCCTGCAGGTGGTTGATCTGTGCAGTCAGCAGTGCTACCTGAACTTCGGT     <  SRR4302543.1195971/94‑1 (MQ=60)
                                                                                         GACCGCGACGGCTGTGGTGATCTTTTTTGTGCTCTGCAAAGTGGCCCTGCAGGTGGTTGATCTGTGCAGTCAGCAGTGCTACCTGAACTTCGGT     <  SRR4302543.3466238/94‑1 (MQ=60)
                                                                                         GACCGCGACGGCTGTGGTGATCTTTTTTGTGCTCTGCAAAGTGGCCCTGCAGGTGGTTGATCTGTGCAGTCAGCAGTGCTACCTGAACTTCGGT     >  SRR4302543.5530462/1‑94 (MQ=60)
                                                                                          ACCGCGACGGCTGTGGTGATCTTTTTTGTGCTCTGCAAAGTGGCCCTGCAGGTGGTTGATCTGTGCAGTCAGCAGTGCTACCTGAACTTCGGTA    >  SRR4302543.7320601/1‑94 (MQ=60)
                                                                                            CGCGACGGCTGTGGTGATCTTTTTTGTGCTCTGCAAAGTGGCCCTGCAGGTGGTTGATCTGTGCAGTCAGCAGTGCTACCTGAACTTCGGTAGA  <  SRR4302543.3903551/94‑1 (MQ=60)
                                                                                             |                                                                                            
CAGGCGCTCGATGAGCTGGGTGTAACGTGCTACGTCTTTACGTTTCAGGTAGTCGAGCAGTTTACGACGCTGAGAAACCATGCGCAGCAGACCACGACGGCTGTGGTGATCTTTTTTGTGCTCTGCAAAGTGGCCCTGCAGGTGGTTGATCTGTGCAGTCAGCAGTGCTACCTGAACTTCGGTAGA  >  NC_000913/3309071‑3309256

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑