Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 1873 183.1 9376148 98.2% 9207377 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,309,164 A→G R54R (CGT→CGC)  rpsO ← 30S ribosomal subunit protein S15

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,309,1640AG100.0% 630.7 / NA 199R54R (CGT→CGC) rpsO30S ribosomal subunit protein S15
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (102/97);  total (102/97)

Only 100 of 215 total aligned reads displayed.

GCGCTCGATGAGCTGGGTGTAACGTGCTACGTCTTTACGTTTCAGGTAGTCGAGCAGTTTACGACGCTGAGAAACCATGCGCAGCAGACCACGACGGCTGTGGTGATCTTTTTTGTGCTCTGCAAAGTGGCCCTGCAGGTGGTTGATCTGTGCAGTCAGCAGTGCTACCTGAACTTCGGT  >  NC_000913/3309074‑3309253
                                                                                          |                                                                                         
 cgcTCGATGAGCTGGGTGTAACGTGCTACGTCTTTACGTTTCAGGTAGTCGAGCAGTTTACGACGCTGAGAAACCATGCGCAGCAGACCgcga                                                                                        <  1:1322713/93‑1 (MQ=255)
  gcTCGATGAGCTGGGTGTAACGTGCTACGTCTTTACGTTTCAGGTAGTCGAGCAGTTTACGACGCTGAGAAACCATGCGCAGCAGACCgcgac                                                                                       >  1:1118133/1‑93 (MQ=255)
  gcTCGATGAGCTGGGTGTAACGTGCTACGTCTTTACGTTTCAGGTAGTCGAGCAGTTTACGACGCTGAGAAACCATGCGCAGCAGACCgcgac                                                                                       <  1:4391929/93‑1 (MQ=255)
  gcTCGATGAGCTGGGTGTAACGTGCTACGTCTTTACGTTTCAGGTAGTCGAGCAGTTTACGACGCTGAGAAACCATGCGCAGCAGACCgcgac                                                                                       >  2:1346262/1‑93 (MQ=255)
  gcTCGATGAGCTGGGTGTAACGTGCTACGTCTTTACGTTTCAGGTAGTCGAGCAGTTTACGACGCTGAGAAACCATGCGCAGCAGACCgcgac                                                                                       <  2:3535988/93‑1 (MQ=255)
  gcTCGATGAGCTGGGTGTAACGTGCTACGTCTTTACGTTTCAGGTAGTCGAGCAGTTTACGACGCTGAGAAACCATGCGCAGCAGACCgcgac                                                                                       <  2:3722185/93‑1 (MQ=255)
      gATGAGCTGGGTGTAACGTGCTACGTCTTTACGTTTCAGGTAGTCGAGCAGTTTACGACGCTGAGAAACCATGCGCAGCAGACCGCGACGGCt                                                                                   >  2:2428962/1‑93 (MQ=255)
        tGAGCTGGGTGTAACGTGCTACGTCTTTACGTTTCAGGTAGTCGAGCAGTTTACGACGCTGAGAAACCATGCGCAGCAGACCGCGACGGCtgt                                                                                 <  2:885447/93‑1 (MQ=255)
           gCTGGGTGTAACGTGCTACGTCTTTACGTTTCAGGTAGTCGAGCAGTTTACGACGCTGAGAAACCATGCGCAGCAGACCGCGACGGCTgtggt                                                                              <  1:3272917/93‑1 (MQ=255)
             tGGGTGTAACGTGCTACGTCTTTACGTTTCAGGTAGTCGAGCAGTTTACGACGCTGAGAAACCATGCGCAGCAGACCGCGACGGCTGTGGTGa                                                                            <  1:3522629/93‑1 (MQ=255)
              gggTGTAACGTGCTACGTCTTTACGTTTCAGGTAGTCGAGCAGTTTACGACGCTGAGAAACCATGCGCAGCAGACCGCGACGGCTGTGGTGAt                                                                           >  1:2729827/1‑93 (MQ=255)
                 tgtAACGTGCTACGTCTTTACGTTTCAGGTAGTCGAGCAGTTTACGACGCTGAGAAACCATGCGCAGCAGACCGCGACGGCTGTGGTGATCtt                                                                        <  1:4028158/93‑1 (MQ=255)
                  gtAACGTGCTACGTCTTTACGTTTCAGGTAGTCGAGCAGTTTACGACGCTGAGAAACCATGCGCAGCAGACCGCGACGGCTGTGGTGATCttt                                                                       >  2:3851498/1‑93 (MQ=255)
                   tgaCGTGCTACGTCTTTACGTTTCAGGTAGCCGAGCAGTTTACGACGCTGAGAAACCACGCGCAGCAGACCGCGACGGCTGTGGTGATCtttt                                                                      >  1:4529109/3‑93 (MQ=255)
                    aaCGTGCTACGTCTTTACGTTTCAGGTAGTCGAGCAGTTTACGACGCTGAGAAACCATGCGCAGCAGACCGCGACGGCTGTGGTGATCttttt                                                                     >  1:1296752/1‑93 (MQ=255)
                    aaCGTGCTACGTCTTTACGTTTCAGGTAGTCGAGCAGTTTACGACGCTGAGAAACCATGCGCAGCAGACCGCGACGGCTGTGGTGATCttttt                                                                     >  2:2911489/1‑93 (MQ=255)
                    aaCGTGCTACGTCTTTACGTTTCAGGTAGTCGAGCAGTTTACGACGCTGAGAAACCATGCGCAGCAGACCGCGACGGCTGTGGTGATCttttt                                                                     <  1:3102219/93‑1 (MQ=255)
                    aaCGTGCTACGTCTTTACGTTTCAGGTAGTCGAGCAGTTTACGACGCTGAGAAACCATGCGCAGCAGACCGCGACGGCTGTGGTGATCttttt                                                                     <  2:1802350/93‑1 (MQ=255)
                     aCGTGCTACGTCTTTACGTTTCAGGTAGTCGAGCAGTTTACGACGCTGAGAAACCATGCGCAGCAGACCGCGACGGCTGTGGTGATCtttttt                                                                    <  2:3973747/93‑1 (MQ=255)
                      cGTGCTACGTCTTTACGTTTCAGGTAGTCGAGCAGTTTACGACGCTGAGAAACCATGCGCAGCAGACCGCGACGGCTGTGGTGATCTTTTTtg                                                                   <  1:2990623/93‑1 (MQ=255)
                      cGTGCTACGTCTTTACGTTTCAGGTAGTCGAGCAGTTTACGACGCTGAGAAACCATGCGCAGCAGACCGCGACGGCTGTGGTGATCTTTTTtg                                                                   >  2:4469182/1‑93 (MQ=255)
                        tGCTACGTCTTTACGTTTCAGGTAGTCGAGCAGTTTACGACGCTGAGAAACCATGCGCAGCAGACCGCGACGGCTGTGGTGATCTTTTTtgtg                                                                 <  1:1483015/93‑1 (MQ=255)
                         gCTACGTCTTTACGTTTCAGGTAGTCGAGCAGTTTACGACGCTGAGAAACCATGCGCAGCAGACCGCGACGGCTGTGGTGATCTTTTTTGTGc                                                                >  1:2262985/1‑93 (MQ=255)
                          cTACGTCTTTACGTTTCAGGTAGTCGAGCAGTTTACGACGCTGAGAAACCATGCGCAGCAGACCGCGACGGCTGTGGTGATCTTTTTTGTGct                                                               <  1:2367900/93‑1 (MQ=255)
                           tACGTCTTTACGTTTCAGGTAGTCGAGCAGTTTACGACGCTGAGAAACCATGCGCAGCAGACCGCGACGGCTGTGGTGATCTTTTTTGTGctc                                                              >  1:4537664/1‑93 (MQ=255)
                           tACGTCTTTACGTTTCAGGTAGTCGAGCAGTTTACGACGCTGAGAAACCATGCGCAGCAGACCGCGACGGCTGTGGTGATCTTTTTTGTGctc                                                              >  1:753216/1‑93 (MQ=255)
                           tACGTCTTTACGTTTCAGGTAGTCGAGCAGTTTACGACGCTGAGAAACCATGCGCAGCAGACCGCGACGGCTGTGGTGATCTTTTTTGTGctc                                                              >  2:3689467/1‑93 (MQ=255)
                            tcGTCTTTACGTTTCAGGTAGTCGAGCAGTTTACGACGCTGAGAAACCATGCGCAGCAGACCGCGACGGCTGTGGTGATCTTTTTTGTGctct                                                             <  1:231083/92‑1 (MQ=255)
                            aCGTCTTTACGTTTCAGGTAGTCGAGCAGTTTACGACGCTGGGAAACCATGCGCAGCAGACCGCGACGGCTGTGGTGATCTTTTTTGTGctct                                                             >  2:1928320/1‑93 (MQ=255)
                            aCGTCTTTACGTTTCAGGTAGTCGAGCAGTTTACGACGCTGAGAAACCATGCGCAGCAGACCGCGACGGCTGTGGTGATCTTTTTTGTGctct                                                             >  1:673020/1‑93 (MQ=255)
                            aCGTCTTTACGTTTCAGGTAGTCGAGCAGTTTACGACGCTGAGAAACCATGCGCAGCAGACCGCGACGGCTGTGGTGATCTTTTTTGTGctct                                                             <  2:2866914/93‑1 (MQ=255)
                            aCGTCTTTACGTTTCAGGTAGTCGAGCAGTTTACGACGCTGAGAAACCATGCGCAGCAGACCGCGACGGCTGTGGTGATCTTTTTTGTGctct                                                             <  1:1636861/93‑1 (MQ=255)
                             cGTCTTTACGTTTCAGGTAGTCGAGCAGTTTACGACGCTGAGAAACCATGCGCAGCAGACCGCGACGGCTGTGGTGATCTTTTTTGTGCTCTg                                                            >  1:1243445/1‑93 (MQ=255)
                               tCTTTACGTTTCAGGTAGTCGAGCAGTTTACGACGCTGAGAAACCATGCGCAGCAGACCGCGACGGCTGTGGTGATCTTTTTTGTGCTCTGCa                                                          >  2:2159251/1‑93 (MQ=255)
                               tCTTTACGTTTCAGGTAGGCGAGCAGTTTACGACGCTGAGAAACCATGCCCAGCAGACCGCGACGGCCGGGGGGGTCTTTTTTGTGCTCTGCa                                                          >  2:363506/1‑93 (MQ=255)
                                 tttACGTTTCAGGTAGTCGAGCAGTTTACGACGCTGAGAAACCATGCGCAGCAGACCGCGACGGCTGTGGTGATCTTTTTTGTGCTCTGCaaa                                                        >  2:3259386/1‑93 (MQ=255)
                                 tttACGTTTCAGGTAGTCGAGCAGTTTACGACGCTGAGAAACCATGCGCAGCAGACCGCGACGGCTGTGGTGATCTTTTTTGTGCTCTGCaaa                                                        <  1:1431926/93‑1 (MQ=255)
                                 tttACGTTTCAGGTAGTCGAGCAGTTTACGACGCTGAGAAACCATGCGCAGCAGACCGCGACGGCTGTGGTGATCTTTTTTGTGCTCTGCaaa                                                        <  2:1253956/93‑1 (MQ=255)
                                  ttACGTTTCAGGTAGTCGAGCAGTTTACGACGCTGAGAAACCATGCGCAGCAGACCGCGACGGCTGTGGTGATCTTTTTTGTGCTCTGCACAg                                                       >  1:141173/1‑93 (MQ=255)
                                  ttACGTTTCAGGTAGTCGAGCAGTTTACGACGCTGAGAAACCATGCGCAGCAGACCGCGACGGCTGTGGTGATCTTTTTTGTGCTCTGCAAAg                                                       >  1:2526430/1‑93 (MQ=255)
                                   tACGTTTCAGGTAGTCGAGCAGTTTACGACGCTGAGAAACCATGCGCAGCAGACCGCGACGGCTGTGGTGATCTTTTTTGTGCTCTGCAAAGt                                                      <  1:4569229/93‑1 (MQ=255)
                                   tACGTTTCAGGTAGTCGAGCAGTTTACGACGCTGAGAAACCATGCGCAGCAGACCGCGACGGCTGTGGTGATCTTTTTTGTGCTCTGCAAAGt                                                      <  1:3205679/93‑1 (MQ=255)
                                    aCGTTTCAGGTAGTCGAGCAGTTTACGACGCTGAGAAACCATGCGCAGCAGACCGCGACGGCTGTGGTGATCTTTTTTGTGCTCTGCAAAGTg                                                     >  1:1025911/1‑93 (MQ=255)
                                    aCGTTTCAGGTAGTCGAGCAGTTTACGACGCTGAGAAACCATGCGCAGCAGACCGCGACGGCTGTGGTGATCTTTTTTGTGCTCTGCAAAGTg                                                     <  2:1532705/93‑1 (MQ=255)
                                      gTTTCAGGTAGTCGAGCAGTTTACGACGCTGAGAAACCATGCGCAGCAGACCGCGACGGCTGTGGTGATCTTTTTTGTGCTCTGCAAAGTGGc                                                   >  1:2342297/1‑93 (MQ=255)
                                      gTTTCAGGTAGTCGAGCAGTTTACGACGCTGAGAAACCATGCGCAGCAGACCGCGACGGCTGTGGTGATCTTTTTTGTGCTCTGCAAAGTGGc                                                   >  1:4108914/1‑93 (MQ=255)
                                        ttCAGGTAGTCGAGCAGTTTACGACGCTGAGAAACCATGCGCAGCAGACCGCGACGGCTGTGGTGATCTTTTTTGTGCTCTGCAAAGTGGccc                                                 <  2:722531/93‑1 (MQ=255)
                                            ggTAGTCGAGCAGTTTACGACGCTGAGAAACCATGCGCAGCAGACCGCGACGGCTGTGGTGATCTTTTTTGTGCTCTGCAAAGTGGCCCTGCa                                             >  2:3457909/1‑93 (MQ=255)
                                            ggTAGTCGAGCAGTTTACGACGCTGAGAAACCATGCGCAGCAGACCGCGACGGCTGTGGTGATCTTTTTTGTGCTCTGCAAAGTGGCCCTGCa                                             <  2:3943676/93‑1 (MQ=255)
                                            ggTAGTCGAGCAGTTTACGACGCTGAGAAACCATGCGCAGCAGACCGCGACGGCTGTGGTGATCTTTTTTGTGCTCTGCAAAGTGGCCCTGCa                                             <  1:3152583/93‑1 (MQ=255)
                                            ggTAGTCGAGCAGTTTACGACGCTGAGAAACCATGCGCAGCAGACCGCGACGGCTGTGGTGATCTTTTTTGTGCTCTGCAAAGTGGCCCTGCa                                             >  1:3819705/1‑93 (MQ=255)
                                               aGTCGAGCAGTTTACGACGCTGAGAAACCATGCGCAGCAGACCGCGACGGCTGTGGTGATCTTTTTTGTGCTCTGCAAAGTGGCCCTGCAggt                                          >  1:4253142/1‑93 (MQ=255)
                                               aGTCGAGCAGTTTACGACGCTGAGAAACCATGCGCAGCAGACCGCGACGGCTGTGGTGATCTTTTTTGTGCTCTGCAAAGTGGCCCTGCAggt                                          <  1:986122/93‑1 (MQ=255)
                                               aGTCGAGCAGTTTACGACGCTGAGAAACCATGCGCAGCAGACCGCGACGGCTGTGGTGATCTTTTTTGTGCTCTGCAAAGTGGCCCTGCAggt                                          <  2:4334605/93‑1 (MQ=255)
                                               aGTCGAGCAGTTTACGACGCTGAGAAACCATGCGCAGCAGACCGCGACGGCTGTGGTGATCTTTTTTGTGCTCTGCAAAGTGGCCCTGCAggt                                          <  1:1579162/93‑1 (MQ=255)
                                               aGTCGAGCAGTTTACGACGCTGAGAAACCATGCGCAGCAGACCGCGACGGCTGTGGTGATCTTTTTTGTGCTCTGCAAAGTGGCCCTGCAggt                                          <  2:3175309/93‑1 (MQ=255)
                                                 tCGAGCAGTTTACGACGCTGAGAAACCATGCGCAGCAGACCGCGACAGCTGTGGTGATCTTTTTTGTGCTCTGCAAAGTGGCCCTGCAggtgg                                        <  2:2965638/93‑1 (MQ=255)
                                                  cGAGCAGTTTACGACGCTGAGAAACCATGCGCAGCAGACCGCGACGGCTGTGGTGATCTTTTTTGTGCTCTGCAAAGTGGCCCTGCAggtggt                                       >  2:1768408/1‑93 (MQ=255)
                                                   gAGCAGTTTACGACGCTGAGAAACCATGCGCAGCAGACCGCGACGGCTGTGGTGATCTTTTTTGTGCTCTGCAAAGTGGCCCTGCAGGTGGtt                                      >  1:2691847/1‑93 (MQ=255)
                                                    aGCAGTTTACGACGCTGAGAAACCATGCGCAGCAGACCGCGACGGCTGTGGTGATCTTTTTTGTGCTCTGCAAAGTGGCCCTGCAGGTGGTTg                                     >  1:484582/1‑93 (MQ=255)
                                                     gCAGTTTACGACGCTGAGAAACCATGCGCAGCAGACCGCGACGGCTGTGGTGATCTTTTTTGTGCTCTGCAAAGTGGCCCTGCAGGTGGTTGa                                    <  2:648508/93‑1 (MQ=255)
                                                     gCAGTTTACGACGCTGAGAAACCATGCGCAGCAGACCGCGACGGCTGTGGTGATCTTTTTTGTGCTCTGCAAAGTGGCCCTGCAGGTGGTTGa                                    >  2:1630671/1‑93 (MQ=255)
                                                       aGTTTACGACGCTGAGAAACCATGCGCAGCAGACCGCGACGGCTGTGGTGATCTTTTTTGTGCTCTGCAAAGTGGCCCTGCAGGTGGTTGATc                                  <  2:2571404/93‑1 (MQ=255)
                                                        gTTTACGACGCTGAGAAACCATGCGCAGCAGACCGCGACGGCTGTGGTGATCTTTTTTGTGCTCTGCAAAGTGGCCCTGCAGGTGGTTGATCt                                 >  1:1263837/1‑93 (MQ=255)
                                                         tttACGACGCTGAGAAACCATGCGCAGCAGACCGCGACGGCTGTGGTGATCTTTTTTGTGCTCTGCAAAGTGGCCCTGCAGGTGGTTGCTCtg                                >  1:835137/1‑93 (MQ=255)
                                                         tttACGACGCTGAGAAACCATGCGCAGCAGACCGCGACGGCTGTGGTGATCTTTTTTGTGCTCTGCAAAGTGGCCCTGCAGGTGGTTGATCtg                                >  1:2511353/1‑93 (MQ=255)
                                                          ttACGACTCTAAGAACCGATGCGCAGCAGACCGCGACGGCTGTGGTGATCTTTTTTGTGCTCTGCAAAGTGGCTCTGCATGTGGTTGATCtgt                               <  2:3003754/93‑1 (MQ=255)
                                                          ttACGACGCTGAGAAACCATGCGCAGCAGACCGCGACGGCTGTGGTGATCTTTTTTGTGCTCTGCAAAGTGGCCCTGCAGGTGGTTGATCtgt                               <  2:2709552/93‑1 (MQ=255)
                                                          ttACGACGCTGAGAAACCATGCGCAGCAGACCGCGACGGCTGTGGTGATCTTTTTTGTGCTCTGCAAAGTGGCCCTGCAGGTGGTTGATCtgt                               <  2:1960409/93‑1 (MQ=255)
                                                            acgacgCTGAGAAACCATGCGCAGCAGCCCGCGACGGCTGTGGTGATCTTTTTTGTGCTCTGCAAAGTGGCACTGCAGGTGGTTGATCTGTGc                             <  2:231545/93‑1 (MQ=255)
                                                            acgacgCTGAGAAACCATGCGCAGCAGACCGCGACGGCTGTGGTGATCTTTTTTGTGCTCTGCAAAGTGGCCCTGCAGGTGGTTGATCTGTGc                             >  2:1539836/1‑93 (MQ=255)
                                                             cgacgCTGAGAAACCATGCGCAGCAGACCGCGACGGCTGTGGTGATCTTTTTTGTGCTCTGCAAAGTGGCCCTGCAGGTGGTTGATCTGTGCa                            <  2:3304569/93‑1 (MQ=255)
                                                             cgacgCTGAGAAACCATGCGCAGCAGACCGCGACGGCTGTGGTGATCTTTTTTGTGCTCTGCAAAGTGGCCCTGCAGGTGGTTGATCTGTGCa                            <  1:734534/93‑1 (MQ=255)
                                                              gacgCTGAGAAACCATGCGCAGCAGACCGCGACGGCTGTGGTGATCTTTTTTGTGCTCTGCAAAGTGGCCCTGCAGGTGGTTGATCTGTGCAg                           <  1:4145093/93‑1 (MQ=255)
                                                              gacgCTGAGAAACCATGCGCAGCAGACCGCGACGGCTGTGGTGATCTTTTTTGTGCTCTGCAAAGTGGCCCTGCAGGTGGTTGATCTGTGCAg                           >  1:685847/1‑93 (MQ=255)
                                                                cgCTGAGAAACCATGCGCAGCAGACCGCGACGGCTGTGGTGATCTTTTTTGTGCTCTGCAAAGTGGCCCTGCAGGTGGTTGATCTGTGCAGTc                         <  1:1962354/93‑1 (MQ=255)
                                                                 gCTGAGAAACCATGCGCAGCAGACCGCGACGGCTGTGGTGATCTTTTTTGTGCTCTGCAAAGTGGCCCTGCAGGTGGTTGATCTGTGCAGTca                        <  1:1216063/93‑1 (MQ=255)
                                                                 gCTGAGAAACCATGCGCAGCAGACCGCGACGGCTGTGGTGATCTTTTTTGTGCTCTGCAAAGTGGCCCTGCAGGTGGTTGATCTGTGCAGTca                        >  2:419029/1‑93 (MQ=255)
                                                                  cTGAGAAACCATGCGCAGCAGACCGCGACGGCTGTGGTGATCTTTTTTGTGCTCTGCAAAGTGGCCCTGCAGGTGGTTGATCTGTGCAGTcag                       >  2:602563/1‑93 (MQ=255)
                                                                   tGAGAAACCATGCGCAGCAGACCGCGACGGCTGTGGTGATCTTTTTTGTGCTCTGCAAAGTGGCCCTGCAGGTGGTTGATCTGTGCAGTcagc                      >  2:825515/1‑93 (MQ=255)
                                                                   tGAGAAACCATGCGCAGCAGACCGCGACGGCTGTGGTGATCTTTTTTGTGCTCTGCAAAGTGGCCCTGCAGGTGGTTGATCTGTGCAGTcagc                      >  2:960686/1‑93 (MQ=255)
                                                                   tGAGAAACCATGCGCAGCAGACCGCGACGGCTGTGGTGATCTTTTTTGTGCTCTGCAAAGTGGCCCTGCAGGTGGTTGATCTGTGCAGTcagc                      >  2:438135/1‑93 (MQ=255)
                                                                    gagaAACCATGCGCAGCAGACCGCGAGGGCTGTGGTGATCTTTTTTGTGCTCTGCAAAGTGGCCCTGCAGGTGGTTGATCTGTGCAGTcagca                     >  2:2752598/1‑93 (MQ=255)
                                                                    gagaAACCATGCGCAGCAGACCGCGACGGCTGTGGTGATCTTTTTTGTGCTCTGCAAAGTGGCCCTGCAGGTGGTTGATCTGTGCAGTcagca                     >  1:1149848/1‑93 (MQ=255)
                                                                    gagaAACCATGCGCAGCAGACCGCGACGGCTGTGGTGATCTTTTTTGTGCTCTGCAAAGTGGCCCTGCAGGTGGTTGATCTGTGCAGTcagca                     >  2:1032261/1‑93 (MQ=255)
                                                                        aaCCATGCGCAGCAGACCGCGACGGCTGTGGTGATCTTTTTTGTGCTCTGCAAAGTGGCCCTGCAGGTGGTTGATCTGTGCAGTCAGCAGTGc                 >  2:1435317/1‑93 (MQ=255)
                                                                         aCCATGCGCAGCAGACCGCGACGGCTGTGGTGATCTTTTTTGTGCTCTGCAAAGTGGCCCTGCAGGTGGTTGATCTGTGCAGTCAGCAGTGCt                >  1:3398115/1‑93 (MQ=255)
                                                                                 cagcagACCGCGACGGCTGTGGTGATCTTTTTTGTGCTCTGCAAAGTGGCCCTGCAGGTGGTTGATCTGTGCAGTCAGCAGTGCTACCTGAAc        >  1:1667678/1‑93 (MQ=255)
                                                                                 cagcagACCGCGACGGCTGTGGTGATCTTTTTTGTGCTCTGCAAAGTGGCCCTGCAGGTGGTTGATCTGTGCAGTCAGCAGTGCTACCTGAAc        <  2:1170675/93‑1 (MQ=255)
                                                                                 cagcagACCGCGACGGCTGTGGTGATCTTTTTTGTGCTCTGCAAAGTGGCCCTGCAGGTGGTTGATCTGTGCAGTCAGCAGTGCTACCTGAAc        <  1:3342421/93‑1 (MQ=255)
                                                                                 cagcagACCGCGACGGCTGTGGTGATCTTTTTTGTGCTCTGCAAAGTGGCCCTGCAGGTGGTTGATCTGTGCAGTCAGCAGTGCTACCTGAAc        >  1:1994579/1‑93 (MQ=255)
                                                                                 cagcagACCGCGACGGCTGTGGTGATCTTTTTTGTGCTCTGCAAAGTGGCCCTGCAGGTGGTTGATCTGTGCAGTCAGCAGTGCTACCTGAAc        <  2:2098280/93‑1 (MQ=255)
                                                                                 cagcagACCGCGACGGCTGTGGTGATCTTTTTTGTGCTCTGCAAAGTGGCCCTGCAGGTGGTTGATCTGTGCAGTCAGCAGTGCTACCTGAAc        <  1:4447712/93‑1 (MQ=255)
                                                                                  agcagACCGCGACGGCTGTGGTGATCTTTTTTGTGCTCTGCAAAGTGGCCCTGCAGGTGGTTGATCTGTGCAGTCAGCAGTGCTACCTGAACt       >  1:2551774/1‑93 (MQ=255)
                                                                                   gcagACCGCGACGGCTGTGGTGATCTTTTTTGTGCTCTGCAGAGTGGCCCTGCAGGTGGTTGATCTGTGCAGTCAGCAGTGCTACCTGAACtt      >  1:379766/1‑93 (MQ=255)
                                                                                   gcagACCGCGACGGCTGTGGTGATCTTTTTTGTGCTCTGCAAAGTGGCCCTGCAGGTGGTTGATCTGTGCAGTCAGCAGTGCTACCTGAACtt      >  2:35303/1‑93 (MQ=255)
                                                                                    cagACCGCGACGGCTGTGGTGATCTTTTTTGTGCTCTGCAAAGTGGCCCTGCAGGTGGTTGATCTGTGCAGTCAGCAGTGCTACCTGAACTTc     <  2:526477/93‑1 (MQ=255)
                                                                                      gACCGCGACGGCTGTGGTGATCTTTTTTGTGCTCTGCAAAGTGGCCCTGCAGGTGGTTGATCTGTGCAGTCAGCAGTGCTACCTGAACTTCgg   >  2:3740130/1‑93 (MQ=255)
                                                                                      gACCGCGACGGCTGTGGTGATCTTTTTTGTGCTCTGCAAAGTGGCCCTGCAGGTGGTTGATCTGTGCAGTCAGCAGTGCTACCTGAACTTCgg   >  1:4347746/1‑93 (MQ=255)
                                                                                       aCCGCGACGGCTGTGGTGATCTTTTTTGTGCTCTGCAAAGTGGCCCTGCAGGTGGTTGATCTGTGCAGTCAGCAGTGCTACCTGAACTTCGGt  <  1:1821133/93‑1 (MQ=255)
                                                                                          |                                                                                         
GCGCTCGATGAGCTGGGTGTAACGTGCTACGTCTTTACGTTTCAGGTAGTCGAGCAGTTTACGACGCTGAGAAACCATGCGCAGCAGACCACGACGGCTGTGGTGATCTTTTTTGTGCTCTGCAAAGTGGCCCTGCAGGTGGTTGATCTGTGCAGTCAGCAGTGCTACCTGAACTTCGGT  >  NC_000913/3309074‑3309253

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CAGGCGCTCGATGAGCTGGGTGTAACGTGCTACGTCTTTACGTTTCAGGTAGTCGAGCAGTTTACGACGCTGAGAAACCATGCGCAGCAGACCACGACGGCTGTGGTGATCTTTTTTGTGCTCTGCAAAGTGGCCCTGCAGGTGGTTGATCTGTGCAGTCAGCAGTGCTACCTGAACTTCGGTAGAA  >  NC_000913/3309071‑3309257
                                                                                             |                                                                                             
CAGGCGCTCGATGAGCTGGGTGTAACGTGCTACGTCTTTACGTTTCAGGTAGTCGAGCAGTTTACGACGCTGAGAAACCATGCGCAGCAGACCG                                                                                               <  SRR4302535.1651542/94‑1 (MQ=60)
CAGGCGCTCGATGAGCTGGGTGTAACGTGCTACGTCTTTACGTTTCAGGTAGTCGAGCAGTTTACGACGCTGAGAAACCATGCGCAGCAGACCG                                                                                               <  SRR4302535.842554/94‑1 (MQ=60)
  GGCGCTCGATGAGCTGGGTGTAACGTGCTACGTCTTTACGTTTCAGGTAGTCGAGCAGTTTACGACGCTGAGAAACCATGCGCAGCAGACCGCG                                                                                             <  SRR4302535.1608142/94‑1 (MQ=60)
  GGCGCTCGATGAGCTGGGTGTAACGTGCTACGTCTTTACGTTTCAGGTAGTCGAGCAGTTTACGACGCTGAGAAACCATGCGCAGCAGACCGCG                                                                                             <  SRR4302535.759302/94‑1 (MQ=60)
    CGCTCGATGAGCTGGGTGTAACGTGCTACGTCTTTACGTTTCAGGTAGTCGAGCAGTTTACGACGCTGAGAAACCATGCGCAGCAGACCGCGAC                                                                                           >  SRR4302535.1126109/1‑94 (MQ=60)
    CGCTCGATGAGCTGGGTGTAACGTGCTACGTCTTTACGTTTCAGGTAGTCGAGCAGTTTACGACGCTGAGAAACCATGCGCAGCAGACCGCGAC                                                                                           <  SRR4302535.1332154/94‑1 (MQ=60)
     GCTCGATGAGCTGGGTGTAACGTGCTACGTCTTTACGTTTCAGGTAGTCGAGCAGTTTACGACGCTGAGAAACCATGCGCAGCAGACCGCGACG                                                                                          <  SRR4302535.684845/94‑1 (MQ=60)
     GCTCGATGAGCTGGGTGTAACGTGCTACGTCTTTACGTTTCAGGTAGTCGAGCAGTTTACGACGCTGAGAAACCATGCGCAGCAGACCGCGACG                                                                                          >  SRR4302535.756797/1‑94 (MQ=60)
     GCTCGATGAGCTGGGTGTAACGTGCTACGTCTTTACGTTTCAGGTAGTCGAGCAGTTTACGACGCTGAGAAACCATGCGCAGCAGACCGCGACG                                                                                          <  SRR4302535.4421979/94‑1 (MQ=60)
     GCTCGATGAGCTGGGTGTAACGTGCTACGTCTTTACGTTTCAGGTAGTCGAGCAGTTTACGACGCTGAGAAACCATGCGCAGCAGACCGCGACG                                                                                          <  SRR4302535.1436360/94‑1 (MQ=60)
       TCGATGAGCTGGGTGTAACGTGCTACGTCTTTACGTTTCAGGTAGTCGAGCAGTTTACGACGCTGAGAAACCATGCGCAGCAGACCGCGACGGC                                                                                        <  SRR4302535.1823395/94‑1 (MQ=60)
        CGATGAGCTGGGTGTAACGTGCTACGTCTTTACGTTTCAGGTAGTCGAGCAGTTTACGACGCTGAGAAACCATGCGCAGCAGACCGCGACGGCT                                                                                       <  SRR4302535.1348279/94‑1 (MQ=60)
             AGCTGGGTGTAACGTGCTACGTCTTTACGTTTCAGGTAGTCGAGCAGTTTACGACGCTGAGAAACCATGCGCAGCAGACCGCGACGGCTGTGGT                                                                                  >  SRR4302535.1270745/1‑94 (MQ=60)
             AGCTGGGTGTAACGTGCTACGTCTTTACGTTTCAGGTAGTCGAGCAGTTTACGACGCTGAGAAACCATGCGCAGCAGACCGCGACGGCTGTGGG                                                                                  >  SRR4302535.1311886/1‑94 (MQ=60)
              GCTGGGTGTAACGTGCTACGTCTTTACGTTTCAGGTAGTCGAGCAGTTTACGACGCTGAGAAACCATGCGCAGCAGACCGCGACGGCTGTGGTG                                                                                 <  SRR4302535.3295651/94‑1 (MQ=60)
               CTGGGTGTAACGTGCTACGTCTTTACGTTTCAGGTAGTCGAGCAGTTTACGACGCTGAGAAACCATGCGCAGCAGACCGCGACGGCTGTGGTGA                                                                                >  SRR4302535.2075777/1‑94 (MQ=60)
               CTGGGTGTAACGTGCTACGTCTTTACGTTTCAGGTAGTCGAGCAGTTTACGACGCTGAGAAACCATGCGCAGCAGACCGCGACGGCTGTGGTGA                                                                                >  SRR4302535.3440445/1‑94 (MQ=60)
                TGGGTGTAACGTGCTACGTCTTTACGTTTCAGGTAGTCGAGCAGTTTACGACGCTGAGAAACCATGCGCAGCAGACCGCGACGGCTGTGGTGAT                                                                               <  SRR4302535.3547093/94‑1 (MQ=60)
                TGGGTGTAACGTGCTACGTCTTTACGTTTCAGGTAGTCGAGCAGTTTACGACGCTGAGAAACCATGCGCAGCAGACCGCGACGGCTGTGGTGAT                                                                               <  SRR4302535.2000697/94‑1 (MQ=60)
                TGGGTGTAACGTGCTACGTCTTTACGTTTCAGGTAGTCGAGCAGTTTACGACGCTGAGAAACCATGCGCAGCAGACCGCGACGGCTGTGGTGAT                                                                               >  SRR4302535.2749316/1‑94 (MQ=60)
                  GGTGTAACGTGCTACGTCTTTACGTTTCAGGTAGTCGAGCAGTTTACGACGCTGAGAAACCATGCGCAGCAGACCGCGACGGCTGTGGTGATGT                                                                             >  SRR4302535.1310582/1‑94 (MQ=60)
                    TGTAACGTGCTACGTCTTTACGTTTCAGGTAGTCGAGCAGTTTACGACGCTGAGAAACCATGCGCAGCAGACCGCGACGGCTGTGGTGATCTTT                                                                           <  SRR4302535.4055720/94‑1 (MQ=60)
                     GTGACGTGCTACGTCTTTACGTTTCAGGTAGCCGAGCAGTTTACGACGCTGAGAAACCACGCGCAGCAGACCGCGACGGCTGTGGTGATCTTTT                                                                          >  SRR4302535.4560120/1‑94 (MQ=60)
                      TAACGTGCTACGTCTTTACGTTTCAGGTAGTCGAGCAGTTTACGACGCTGAGAAACCATGCGCAGCAGACCGCGACGGCTGTGGTGATCTTTTT                                                                         >  SRR4302535.1306003/1‑94 (MQ=60)
                       AACGTGCTACGTCTTTACGTTTCAGGTAGTCGAGCAGTTTACGACGCTGAGAAACCATGCGCAGCAGACCGCGACGGCTGTGGTGATCTTTTTT                                                                        <  SRR4302535.3123813/94‑1 (MQ=60)
                       AACGTGCTACGTCTTTACGTTTCAGGTAGTCGAGCAGTTTACGACGCTGAGAAACCATGCGCAGCAGACCGCGACGGCTGTGGTGATCTTTTTT                                                                        >  SRR4302535.1662751/1‑94 (MQ=60)
                       AACGTGCTACGTCTTTACGTTTCAGGTAGTCGAGCAGTTTACGACGCTGAGAAACCATGCGCAGCAGACCGCGACGGCTGTAGTGATCTTTTTT                                                                        >  SRR4302535.1152917/1‑94 (MQ=60)
                         CGTGCTACGTCTTTACGTTTCAGGTAGTCGAGCAGTTTACGACGCTGAGAAACCATGCGCAGCAGACCGCGACGGCTGTGGTGATCTTTTTTGT                                                                      <  SRR4302535.3011595/94‑1 (MQ=60)
                          GTGCTACGTCTTTACGTTTCAGGTAGTCGAGCAGTTTACGACGCTGAGAAACCATGCGCAGCAGACCGCGACGGCTGTGGTGATCTTTTTTGTG                                                                     >  SRR4302535.1751409/1‑94 (MQ=60)
                          GTGCTACGTCTTTACGTTTCAGGTAGTCGAGCAGTTTACGACGCTGAGAAACCATGCGCAGCAGACCGCGACGGCTGTGGTGATCTTTTTTGTG                                                                     <  SRR4302535.1279037/94‑1 (MQ=60)
                           TGCTACGTCTTTACGTTTCAGGTAGTCGAGCAGTTTACGACGCTGAGAAACCATGCGCAGCAGACCGCGACGGCTGTGGTGATCTTTTTTGTGC                                                                    <  SRR4302535.1493620/94‑1 (MQ=60)
                           TGCTACGTCTTTACGTTTCAGGTAGTCGAGCAGTTTACGACGCTGAGAAACCATGCGCAGCAGACCGCGACGGCTGTGGTGATCTTTTTTGTGC                                                                    >  SRR4302535.2279106/1‑94 (MQ=60)
                             CTACGTCTTTACGTTTCAGGTAGTCGAGCAGTTTACGACGCTGAGAAACCATGCGCAGCAGACCGCGACGGCTGTGGTGATCTTTTTTGTGCTC                                                                  >  SRR4302535.4568707/1‑94 (MQ=60)
                             CTACGTCTTTACGTTTCAGGTAGTCGAGCAGTTTACGACGCTGAGAAACCATGCGCAGCAGACCGCGACGGCTGTGGTGATCTTTTTTGTGCTC                                                                  >  SRR4302535.758518/1‑94 (MQ=60)
                             CTACGTCTTTACGTTTCAGGTAGTCGAGCAGTTTACGACGCTGAGAAACCATGCGCAGCAGACCGCGACGGCTGTGGTGATCTTTTTTGTGCTC                                                                  <  SRR4302535.2384900/94‑1 (MQ=60)
                              TACGTCTTTACGTTTCAGGTAGTCGAGCAGTTTACGACGCTGAGAAACCATGCGCAGCAGACCGCGACGGCTGTGGTGATCTTTTTTGTGCTCT                                                                 >  SRR4302535.677716/1‑94 (MQ=60)
                               TCGTCTTTACGTTTCAGGTAGTCGAGCAGTTTACGACGCTGAGAAACCATGCGCAGCAGACCGCGACGGCTGTGGTGATCTTTTTTGTGCTCTG                                                                <  SRR4302535.232678/94‑1 (MQ=60)
                               ACGTCTTTACGTTTCAGGTAGTCGAGCAGTTTACGACGCTGAGAAACCATGCGCAGCAGACCGCGACGGCTGTGGTGATCTTTTTTGTGCTCTG                                                                <  SRR4302535.1648684/94‑1 (MQ=60)
                               ACGTCTTTACGTTTCAGGTAGTCGAGCAGTTTACGACGCTGAGAAACCATGCGCAGCAGACCGCGACGGCTGTGGTGATCTTTTTTGTGCTCTG                                                                >  SRR4302535.1252325/1‑94 (MQ=60)
                                CGTCTTTACGTTTCAGGTAGTCGAGCAGTTTACGACGCTGAGAAACCATGCGCAGCAGACCGCGACGGCTGTGGTGATCTTTTTTGTGCTCTGC                                                               >  SRR4302535.3221523/1‑94 (MQ=60)
                                 GTCTTTACGTTTCAGGTAGTCGAGCAGTTTACGACGCTGAGAAACCATGCGCAGCAGACCGCGACGGCTGTGGTGATCTTTTTTGTGCTCTGCA                                                              >  SRR4302535.3063000/1‑94 (MQ=60)
                                    TTTACGTTTCAGGTAGTCGAGCAGTTTACGACGCTGAGAAACCATGCGCAGCAGACCGCGACGGCTGTGGTGATCTTTTTTGTGCTCTGCACAG                                                           >  SRR4302535.142165/1‑94 (MQ=60)
                                    TTTACGTTTCAGGTAGTCGAGCAGTTTACGACGCTGAGAAACCATGCGCAGCAGACCGCGACGGCTGTGGTGATCTTTTTTGTGCTCTGCAAAG                                                           <  SRR4302535.1442147/94‑1 (MQ=60)
                                    TTTACGTTTCAGGTAGTCGAGCAGTTTACGACGCTGAGAAACCATGCGCAGCAGACCGCGACGGCTGTGGTGATCTTTTTTGTGCTCTGCAAAG                                                           <  SRR4302535.4396058/94‑1 (MQ=60)
                                    TTTACGTTTCAGGTAGTCGAGCAGTTTACGACGCTGAGAAACCATGCGCAGCAGACCGCGACGGCTGTGGTGATCTTTTTTGTGCTCTGCAAAG                                                           >  SRR4302535.2544631/1‑94 (MQ=60)
                                    TTTACGTTTCAGGTAGTCGAGCAGTTTACGACGCTGAGAAACCATGCGCAGCAGACCGCGACGGCTGTGGTGATCTTTTTTGTGCTCTGCAAAG                                                           <  SRR4302535.1250923/94‑1 (MQ=60)
                                     TTACGTTTCAGGTAGTCGAGCAGTTTACGACGCTGAGAAACCATGCGCAGCAGACCGCGACGGCTGTGGTGATCTTTTTTGTGCTCTGCAAAGT                                                          <  SRR4302535.4158312/94‑1 (MQ=60)
                                     TTACGTTTCAGGTAGTCGAGCAGTTTACGACGCTGAGAAACCATGCGCAGCAGACCGCGACGGCTGTGGTGATCTTTTTTGTGCTCTGCAAAGT                                                          >  SRR4302535.4560791/1‑94 (MQ=60)
                                      TACGTTTCAGGTAGTCGAGCAGTTTACGACGCTGAGAAACCATGCGCAGCAGACCGCGACGGCTGTGGTGATCTTTTTTGTGCTCTGCAAAGTG                                                         <  SRR4302535.230128/94‑1 (MQ=60)
                                      TACGTTTCAGGTAGTCGAGCAGTTTACGACGCTGAGAAACCATGCGCAGCAGACCGCGACGGCTGTGGTGATCTTTTTTGTGCTCTGCAAAGTG                                                         <  SRR4302535.4600389/94‑1 (MQ=60)
                                      TACGTTTCAGGTAGTCGAGCAGTTTACGACGCTGAGAAACCATGCGCAGCAGACCGCGACGGCTGTGGTGATCTTTTTTGTGCTCTGCAAAGTG                                                         <  SRR4302535.3227879/94‑1 (MQ=60)
                                      TACGTTTCAGGTAGTCGAGCAGTTTACGACGCTGAGAAACCATGCGCAGCAGACCGCGACGGCTGTGGTGATCTTTTTTGTGCTCTGCAAAGTG                                                         >  SRR4302535.1033197/1‑94 (MQ=60)
                                        CGTTTCAGGTAGTCGAGCAGTTTACGACGCTGAGAAACCATGCGCAGCAGACCGCGACGGCTGTGGTGATCTTTTTTGTGCTCTGCAAAGTGGC                                                       >  SRR4302535.2359058/1‑94 (MQ=60)
                                        CGTTTCAGGTAGTCGAGCAGTTTACGACGCTGAGAAACCATGCGCAGCAGACCGCGACGGCTGTGGTGATCTTTTTTGTGCTCTGCAAAGTGGC                                                       >  SRR4302535.4137046/1‑94 (MQ=60)
                                          TTTCAGGTAGTCGAGCAGTTTACGACGCTGAGAAACCATGCGCAGCAGACCGCGACGGCTGTGGTGATCTTTTTTGTGCTCTGCAAAGTGGCCC                                                     <  SRR4302535.3274831/94‑1 (MQ=60)
                                            TCAGGTAGTCGAGCAGTTTACGACGCTGAGAAACCATGCGCAGCAGACCGCGACGGCTTTGGTGATCTTTTTTGTGCTCTGCAAAGTGGCCCTG                                                   >  SRR4302535.186430/1‑94 (MQ=60)
                                             CAGGTAGTCGAGCAGTTTACGACGCTGAGAAACCATGCGCAGCAGACCGCGACGGCTGTGGTGATCTTTTTTGTGCTCTGCAAAGTGGCCCTGC                                                  >  SRR4302535.1059079/1‑94 (MQ=60)
                                              AGGTAGTCGAGCAGTTTACGACGCTGAGAAACCATGCGCAGCAGACCGCGACGGCTGTGGTGATCTTTTTTGTGCTCTGCAAAGTGGCCCTGCA                                                 >  SRR4302535.3846061/1‑94 (MQ=60)
                                               GGTAGTCGAGCAGTTTACGACGCTGAGAAACCATGCGCAGCAGACCGCGACGGCTGTGGTGATCTTTTTTGTGCTCTGCAAAGTGGCCCTGCAG                                                <  SRR4302535.2719428/94‑1 (MQ=60)
                                               GGTAGTCGAGCAGTTTACGACGCTGAGAAACCATGCGCAGCAGACCGCGACGGCTGTGGTGATCTTTTTTGTGCTCTGCAAAGTGGCCCTGCAG                                                <  SRR4302535.3174490/94‑1 (MQ=60)
                                                 TAGTCGAGCAGTTTACGACGCTGAGAAACCATGCGCAGCAGACCGCGACGGCTGTGGTGATCTTTTTTGTGCTCTGCAAAGTGGCCCTGCAGGT                                              <  SRR4302535.2781082/94‑1 (MQ=60)
                                                 TAGTCGAGCAGTTTACGACGCTGAGAAACCATGCGCAGCAGACCGCGACGGCTGTGGTGATCTTTTTTGTGCTCTGCAAAGTGGCCCTGCAGGT                                              >  SRR4302535.4282285/1‑94 (MQ=60)
                                                  AGTCGAGCAGTTTACGACGCTGAGAAACCATGCGCAGCAGACCGCGACGGCTGTGGTGATCTTTTTTGTGCTCTGCAAAGTGGCCCTGCAGGTG                                             <  SRR4302535.993127/94‑1 (MQ=60)
                                                  AGTCGAGCAGTTTACGACGCTGAGAAACCATGCGCAGCAGACCGCGACGGCTGTGGTGATCTTTTTTGTGCTCTGCAAAGTGGCCCTGCAGGTG                                             <  SRR4302535.2465503/94‑1 (MQ=60)
                                                  AGTCGAGCAGTTTACGACGCTGAGAAACCATGCGCAGCAGACCGCGACGGCTGTGGTGATCTTTTTTGTGCTCTGCAAAGTGGCCCTGCAGGTG                                             <  SRR4302535.1590560/94‑1 (MQ=60)
                                                   GTCGAGCAGTTTACGACGCTGAGAAACCATGCGCAGCAGACCGCGACGGCTGTGGTGATCTTTTTTGTGCTCTGCAAAGTGGCCCTGCAGGTGG                                            >  SRR4302535.254359/1‑94 (MQ=60)
                                                     CGAGCAGTTTACGACGCTGAGAAACCATGCGCAGCAGACCGCGACGGCTGTGGTGATCTTTTTTGTGCTCTGCAAAGTGGCCCTGCAGGTGGTT                                          >  SRR4302535.2711089/1‑94 (MQ=60)
                                                     CGAGCAGTTTACGACGCTGAGAAACCATGCGCAGCAGACCGCGACGGCTGTGGTGATCTTTTTTGTGCTCTGCAAAGTGGCCCTGCAGGTGGTT                                          <  SRR4302535.4588546/94‑1 (MQ=60)
                                                     CGAGCAGTTTACGACGCTGAGAAACCATGCGCAGCAGACCGCGACGGCTGTGGTGATCTTTTTTGTGCTCTGCAAAGTGGCCCTGCAGGTGGTT                                          >  SRR4302535.1544920/1‑94 (MQ=60)
                                                      GAGCAGTTTACGACGCTGAGAAACCATGCGCAGCAGACCGCGACGGCTGTGGTGATCTTTTTTGTGCTCTGCAAAGTGGCCCTGCAGGTGGTTG                                         >  SRR4302535.487980/1‑94 (MQ=60)
                                                      GAGCAGTTTACGACGCTGAGAAACCATGCGCAGCAGACCGCGACGGCTGTGGTGATCTTTTTTGTGCTCTGCAAAGTGGCCCTGCAGGTGGTTG                                         >  SRR4302535.3686848/1‑94 (MQ=60)
                                                        GCAGTTTACGACGCTGAGAAACCATGCGCAGCAGACCGCGACGGCTGTGGTGATCTTTTTTGTGCTCTGCAAAGTGGCCCTGCAGGTGGTTGAT                                       >  SRR4302535.1208114/1‑94 (MQ=60)
                                                         CAGTTTACGACGCTGAGAAACCATGCGCAGCAGACCGCGACGGCTGTGGTGATCTTTTTTGTGCTCTGCAAAGTGGCCCTGCAGGTGGTTGATC                                      <  SRR4302535.4285778/94‑1 (MQ=60)
                                                         CAGTTTACGACGCTGAGAAACCATGCGCAGCAGACCGCGACGGCTGTGGTGATCTTTTTTGTGCTCTGCAAAGTGGCCCTGCAGGTGGTTGATC                                      <  SRR4302535.2376732/94‑1 (MQ=60)
                                                         CAGTTTACGACGCTGAGAAACCATGCGCAGCAGACCGCGACGGCTGTGGTGATCTTTTTTGTGCTCTGCAAAGTGGCCCTGCAGGTGGTTGATC                                      >  SRR4302535.4529759/1‑94 (MQ=60)
                                                         CAGTTTACGACGCTGAGAAACCATGCGCAGCAGACCGCGACGGCTGTGGTGATCTTTTTTGTGCTCTGCAAAGTGGCCCTGCAGGTGGTTGATC                                      >  SRR4302535.873142/1‑94 (MQ=60)
                                                          AGTTTACGACGCTGAGAAACCATGCGCAGCAGACCGCGACGGCTGTGGTGATCTTTTTTGTGCTCTGCAAAGTGGCCCTGCAGGTGGTTGATCT                                     >  SRR4302535.1272878/1‑94 (MQ=60)
                                                           GTTTACGACGCTGAGAAACCATGCGCAGCAGACCGCGACGGCTGTGGTGATCTTTTTTGTGCTCTGCAAAGTGGCCCTGCAGGTGGTTGCTCTG                                    >  SRR4302535.840994/1‑94 (MQ=60)
                                                           GTTTACGACGCTGAGAAACCATGCGCAGCAGACCGCGACGGCTGTGGTGATCTTTTTTGTGCTCTGCAAAGTGGCCCTGCAGGTGGTTGATCTG                                    >  SRR4302535.2529453/1‑94 (MQ=60)
                                                             TTACGACGCTGAGAAACCATGCGCAGCAGACCGCGACGGCTGTGGTGATCTTTTTTGTGCTCTGCAAAGTGGCCCTGCAGGTGGCTGATCTGTG                                  <  SRR4302535.2352512/94‑1 (MQ=60)
                                                                CGACGCTGAGAAACCATGCGCAGCAGACCGCGACGGCTGTGGTGATCTTTTTTGTGCTCTGCAAAGTGGCCCTGCAGGTGGTTGATCTGTGCAG                               >  SRR4302535.690651/1‑94 (MQ=60)
                                                                CGACGCTGAGAAACCATGCGCAGCAGACCGCGACGGCTGTGGTGATCTTTTTTGTGCTCTGCAAAGTGGCCCTGCAGGTGGTTGATCTGTGCAG                               <  SRR4302535.739698/94‑1 (MQ=60)
                                                                CGACGCTGAGAAACCATGCGCAGCAGACCGCGACGGCTGTGGTGATCTTTTTTGTGCTCTGCAAAGTGGCCCTGCAGGTGGTTGATCTGTGCAG                               >  SRR4302535.3131700/1‑94 (MQ=60)
                                                                 GACGCTGAGAAACCATGCGCAGCAGACCGCGACGGCTGTGGTGATCTTTTTTGTGCTCTGCAAAGTGGCCCTGCAGGTGGTTGATCTGTGCAGT                              <  SRR4302535.4173477/94‑1 (MQ=60)
                                                                   CGCTGAGAAACCATGCGCAGCAGACCGCGACGGCTGTGGTGATCTTTTTTGTGCTCTGCAAAGTGGCCCTGCAGGTGGTTGATCTGTGCAGTCA                            >  SRR4302535.716319/1‑94 (MQ=60)
                                                                   CGCTGAGAAACCATGCGCAGCAGACCGCGACGGCTGTGGTGATCTTTTTTGTGCTCTGCAAAGTGGCCCTGCAGGTGGTTGATCTGTGCAGTCA                            <  SRR4302535.1976503/94‑1 (MQ=60)
                                                                   CGCTGAGAAACCATGCGCAGCAGACCGCGACGGCTGTGGTGATCTTTTTTGTGCTCTGCAAAGTGGCCCTGCAGGTGGTTGATCTGTGCAGTCA                            >  SRR4302535.3063322/1‑94 (MQ=60)
                                                                    GCTGAGAAACCATGCGCAGCAGACCGCGACGGCTGTGGTGATCTTTTTTGTGCTCTGCAAAGTGGCCCTGCAGGTGGTTGATCTGTGCAGTCAG                           <  SRR4302535.1224787/94‑1 (MQ=60)
                                                                      TGAGAAACCATGCGCAGCAGACCGCGACGGCTGTGGTGATCTTTTTTGTGCTCTGCAAAGTGGCCCTGCAGGTGGTTGATCTGTGCAGTCAGCA                         >  SRR4302535.1158039/1‑94 (MQ=60)
                                                                      TGAGAAACCATGCGCAGCAGACCGCGACGGCTGTGGTGATCTTTTTTGTGCTCTGCAAAGTGGCCCTGCAGGTGGTTGATCTGTGCAGTCAGCA                         >  SRR4302535.3374322/1‑94 (MQ=60)
                                                                       GAGAAACCATGCGCAGCAGACCGCGACGGCTGTGGTGATCTTTTTTGTGCTCTGCAAAGTGGCCCTGCAGGTGGTTGATCTGTGCAGTCAGCAG                        <  SRR4302535.2565482/94‑1 (MQ=60)
                                                                        AGAAACCATGCGCAGCAGACCGCGACGGCTGTGGTGATCTTTTTTGTGCTCTGCAAAGTGGCCCTGCAGGTGGTTGATCTGTGCAGTCAGCAGT                       >  SRR4302535.1487359/1‑94 (MQ=60)
                                                                        AGAAACCATGCGCAGCAGACCGCGACGGCTGTGGTGATCTTTTTTGTGCTCTGCAAAGTGGCCCTGCAGGTGGTTGATCTGTGCAGTCAGCAGT                       >  SRR4302535.1616211/1‑94 (MQ=60)
                                                                        AGAAACCATGCGCAGCAGACCGCGACGGCTGTGGTGATCTTTTTTGTGCTCTGCAAAGTGGCCCTGCAGGTGGTTGATCTGTGCAGTCAGCAGT                       >  SRR4302535.4313162/1‑94 (MQ=60)
                                                                         GAAACCATGCGCAGCAGACCGCGACGGCTGTGGTGATCTTTTTTGTGCTCTGCAAAGTGGCCCTGCAGGTGGTTGATCTGTGCAGTCAGCAGTG                      <  SRR4302535.2602039/94‑1 (MQ=60)
                                                                          AAACCATGCGCAGCAGACCGCGACGGCTGTGGTGATCTTTTTTGTGCTCTGCAAAGTGGCCCTGCAGGTGGTTGATCTGTGCAGTCAGCAGTGC                     >  SRR4302535.409399/1‑94 (MQ=60)
                                                                          AAACCATGCGCAGCAGACCGCGACGGCTGTGGTGATCTTTTTTGTGCTCTGCAAAGTGGCCCTGCAGGTGGTTGATCTGTGCAGTCAGCAGTGC                     >  SRR4302535.665367/1‑94 (MQ=60)
                                                                           AACCATGCGCAGCAGACCGCGACGGCTGTGGTGATCTTTTTTGTGCTCTGCAAAGTGGCCCTGCAGGTGGTTGATCTGTGCAGTCAGCAGTGCT                    >  SRR4302535.3421759/1‑94 (MQ=60)
                                                                             CCATGCGCAGCAGACCGCGACGGCTGTGGTGATCTTTTTTGTGCTCTGCAAAGTGGCCCTGCAGGTGGTTGATCTGTGCAGTCAGCAGTGCTAC                  <  SRR4302535.3841109/94‑1 (MQ=60)
                                                                                   GCAGCAGACCGCGACGGCTGTGGTGATCTTTTTTGTGCTCTGCAAAGTGGCCCTGCAGGTGGTTGATCTGTGCAGTCAGCAGTGCTACCTGAAC            >  SRR4302535.2008943/1‑94 (MQ=60)
                                                                                   GCAGCAGACCGCGACGGCTGTGGTGATCTTTTTTGTGCTCTGCAAAGTGGCCCTGCAGGTGGTTGATCTGTGCAGTCAGCAGTGCTACCTGAAC            >  SRR4302535.1679747/1‑94 (MQ=60)
                                                                                    CAGCAGACCGCGACGGCTGTGGTGATCTTTTTTGTGCTCTGCAAAGTGGCCCTGCAGGTGGTTGATCTGTGCAGTCAGCAGTGCTACCTGAACT           <  SRR4302535.496576/94‑1 (MQ=60)
                                                                                    CAGCAGACCGCGACGGCTGTGGTGATCTTTTTTGTGCTCTGCAAAGTGGCCCTGCAGGTGGTTGATCTGTGCAGTCAGCAGTGCTACCTGAACT           <  SRR4302535.3365711/94‑1 (MQ=60)
                                                                                    CAGCAGACCGCGACGGCTGTGGTGATCTTTTTTGTGCTCTGCAAAGTGGCCCTGCAGGTGGTTGATCTGTGCAGTCAGCAGTGCTACCTGAACT           >  SRR4302535.2570147/1‑94 (MQ=60)
                                                                                    CAGCAGACCGCGACGGCTGTGGTGATCTTTTTTGTGCTCTGCAAAGTGGCCCTGCAGGTGGTTGATCTGTGCAGTCAGCAGTGCTACCTGAACT           <  SRR4302535.4478211/94‑1 (MQ=60)
                                                                                    CAGCAGACCGCGACGGCTGTGGTGATCTTTTTTGTGCTCTGCAAAGTGGCCCTGCAGGTGGTTGATCTGTGCAGTCAGCAGTGCTACCTGAACT           <  SRR4302535.4478210/94‑1 (MQ=60)
                                                                                     AGCAGACCGCGACGGCTGTGGTGATCTTTTTTGTGCTCTGCAGAGTGGCCCTGCAGGTGGTTGATCTGTGCAGTCAGCAGTGCTACCTGAACTT          >  SRR4302535.382395/1‑94 (MQ=60)
                                                                                     AGCAGACCGCGACGGCTGTGGTGATCTTTTTTGTGCTCTGCAAAGTGGCCCTGCAGGTGGTTGATCTGTGCAGTCAGCAGTGCTACCTGAACTT          >  SRR4302535.3989773/1‑94 (MQ=60)
                                                                                     AGCAGACCGCGACGGCTGTGGTGATCTTTTTTGTGCTCTGCAAAGTGGCCCTGCAGGTGGTTGATCTGTGCAGTCAGCAGTGCTACCTGAACTT          >  SRR4302535.2379364/1‑94 (MQ=60)
                                                                                       CAGACCGCGACGGCTGTGGTGATCTTTTTTGTGCTCTGCAAAGTGGCCCTGCAGGTGGTTGATCTGTGCAGTCAGCAGTGCTACCTGAACTTCG        >  SRR4302535.332657/1‑94 (MQ=60)
                                                                                        AGACCGCGACGGCTGTGGTGATCTTTTTTGTGCTCTGCAAAGTGGCCCTGCAGGTGGTTGATCTGTGCAGTCAGCAGTGCTACCTGAACTTCGG       >  SRR4302535.424554/1‑94 (MQ=60)
                                                                                        AGACCGCGACGGCTGTGGTGATCTTTTTTGTGCTCTGCAAAGTGGCCCTGCAGGTGGTTGATCTGTGCAGTCAGCAGTGCTACCTGAACTTCGG       >  SRR4302535.4377491/1‑94 (MQ=60)
                                                                                          ACCGCGACGGCTGTGGTGATCTTTTTTGTGCTCTGCAAAGTGGCCCTGCAGGTGGTTGATCTGTGCAGTTAGCAGTGCTACCTGAACTTCGGTA     <  SRR4302535.4697736/94‑1 (MQ=60)
                                                                                          ACCGCGACGGCTGTGGTGATCTTTTTTGTGCTCTGCAAAGTGGCCCTGCAGGTGGTTGATCTGTGCAGTCAGCAGTGCTACCTGAACTTCGGTA     <  SRR4302535.1834289/94‑1 (MQ=60)
                                                                                          ACCGCGACGGCTGTGGTGATCTTTTTTGTGCTCTGCAAAGTGGCCCTGCAGGTGGTTGATCTGTGCAGTCAGCAGTGCTACCTGAACTTCGGTA     <  SRR4302535.3754736/94‑1 (MQ=60)
                                                                                          ACCGCGACGGCTGTGGTGATCTTTTTTGTGCTCTGCAAAGTGGCCCTGCAGGTGGTTGATCTGTGCAGTCAGCAGTGCTACCTGAACTTCGGTA     <  SRR4302535.77812/94‑1 (MQ=60)
                                                                                            CGCGACGGCTGTGGTGATCTTTTTTGTGCTCTGCAAAGTGGCCCTGCAGGTGGTTGATCTGTGCAGTCAGCAGTGCTACCTGAACTTCGGTAGA   >  SRR4302535.4205837/1‑94 (MQ=60)
                                                                                            CGCGACGGCTGTGGTGATCTTTTTTGTGCTCTGCAAAGTGGCCCTGCAGGTGGTTGATCTGTGCAGTCAGCAGTGCTACCTGAACTTCGGTAGA   <  SRR4302535.4348240/94‑1 (MQ=60)
                                                                                             GCGACGGCTGTGGTGATCTTTTTTGTGCTCTGCAAAGTGGCCCTGCAGGTGGTTGATCTGTGCAGTCAGCAGTGCTACCTGAACTTCGGTAGAA  <  SRR4302535.3789343/94‑1 (MQ=60)
                                                                                             |                                                                                             
CAGGCGCTCGATGAGCTGGGTGTAACGTGCTACGTCTTTACGTTTCAGGTAGTCGAGCAGTTTACGACGCTGAGAAACCATGCGCAGCAGACCACGACGGCTGTGGTGATCTTTTTTGTGCTCTGCAAAGTGGCCCTGCAGGTGGTTGATCTGTGCAGTCAGCAGTGCTACCTGAACTTCGGTAGAA  >  NC_000913/3309071‑3309257

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑