Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 1696 279.3 15874187 97.8% 15524954 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,496,430 T→C I322I (ATT→ATC)  cysG → uroporphyrinogen III methylase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,496,4300TC99.7% 963.3 / ‑5.6 305I322I (ATT→ATC) cysGuroporphyrinogen III methylase
Reads supporting (aligned to +/- strand):  ref base T (0/1);  new base C (131/173);  total (131/174)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.97e-01

Only 100 of 323 total aligned reads displayed.

AGGCAAACGCGTGGTGCGGCTGAAAGGTGGCGATCCGTTTATTTTTGGCCGTGGTGGCGAAGAGCTGGAAACACTGTGCAACGCGGGTATTCCGTTCTCGGTGGTTCCGGGTATTACCGCAGCTTCTGGTTGCTCTGCCTATTCGGGTATTCCACTCACGCATCGCGATTATGCCCAGAG  >  NC_000913/3496340‑3496519
                                                                                          |                                                                                         
 ggCAAACGCGTGGTGCGGCTGAAAGGTGGCGATCCGTTTATTTTTGGCCGTGGTGGCGAAGAGCTGGAAACACTGTGCAACGCGGGTATCCCg                                                                                        >  2:6238104/1‑93 (MQ=255)
    aaaCGCGTGGTGCGGCTGAAAGGTGGCGATCCGTTTATTTTTGGCCGTGGTGGCGAAGAGCTGGAAACACTGTGCAACGCGGGTATCCCGTtc                                                                                     <  2:5853318/93‑1 (MQ=255)
     aaCGCGTGGTGCGGCTGAAAGGTGGCGATCCGTTTATTTTTGGCCGTGGTGGCGAAGAGCTGGAAACACTGTGCAACGCGGGTATCCCGTtct                                                                                    <  1:1790713/93‑1 (MQ=255)
     aaCGCGTGGTGCGGCTGAAAGGTGGCGATCCGTTTATTTTTGGCCGTGGTGGCGAAGAGCTGGAAACACTGTGCAACGCGGGTATCCCGTtct                                                                                    >  1:4441734/1‑93 (MQ=255)
      aCGCGTGGTGCGGCTGAAAGGTGGCGATCCGTTTATTTTTGGCCGTGGTGGCGAAGAGCTGGAAACACTGTGCAACGCGGGTATCCCGTtctc                                                                                   >  2:4071495/1‑93 (MQ=255)
      aCGCGTGGTGCGGCTGAAAGGTGGCGATCCGTTTATTTTTGGCCGTGGTGGCGAAGAGCTGGAAACACTGTGCAACGCGGGTATCCCGTtctc                                                                                   >  1:6045444/1‑93 (MQ=255)
       cgcgTGGTGCGGCTGAAAGGTGGCGATCCGTTTATTTTTGGCCGTGGTGGCGAAGAGCTGGAAACACTGTGCAACGCGGGTATCCCGTTCTCg                                                                                  <  2:4270785/93‑1 (MQ=255)
        gcgTGGTGCGGCTGAAAGGTGGCGATCCGTTTATTTTTGGCCGTGGTGGCGAAGAGCTGGAAACACTGTGCAACGCGGGTATCCCGTTCTCgg                                                                                 >  1:1440300/1‑93 (MQ=255)
        gcgTGGTGCGGCTGAAAGGTGGCGATCCGTTTATTTTTGGCCGTGGTGGCGAAGAGCTGGAAACACTGTGCAACGCGGGTATCCCGTTCTCgg                                                                                 <  2:1210385/93‑1 (MQ=255)
             gtgCGGCTGAAAGGTGGCGATCCGTTTATTTTTGGCCGTGGTGGCGAAGAGCTGGAAACACTGTGCAACGCGGGTATCCCGTTCTCGGTGGtt                                                                            <  2:7112138/93‑1 (MQ=255)
             gtgCGGCTGAAAGGTGGCGATCCGTTTATTTTTGGCCGTGGTGGCGAAGAGCTGGAAACACTGTGCAACGCGGGTATCCCGTTCTCGGTGGtt                                                                            >  2:1889143/1‑93 (MQ=255)
             gtgCGGCTGAAAGGTGGCGATCCGTTTATTTTTGGCCGTGGTGGCGAAGAGCTGGAAACACTGTGCAACGCGGGTATCCCGTTCTCGGTGGtt                                                                            >  1:5457568/1‑93 (MQ=255)
              tgCGGCTGAAAGGTGGCGATCCGTTTATTTTTGGCCGTGGTGGCGAAGAGCTGGAAACACTGTGCAACGCGGGTATCCCGTTCTCGGTGGTTc                                                                           <  2:6738341/93‑1 (MQ=255)
                 ggCTGAAAGGTGGCGATCCGTTTATTTTTGGCCGTGGTGGCGAAGAGCTGGAAACACTGTGCAACGCGGGTATCCCGTTCTCGGTGGTTCCgg                                                                        <  2:609549/93‑1 (MQ=255)
                  gCTGAAAGGTGGCGATCCGTTTATTTTTGGCCGTGGTGGCGAAGAGCTGGAAACACTGTGCAACGCGGGTATCCCGTTCTCGGTGGTTCCggg                                                                       <  1:5701826/93‑1 (MQ=255)
                    tGAAAGGTGGCGATCCGTTTATTTTTGGCCGTGGTGGCGAAGAGCTGGAAACACTGTGCAACGCGGGTATCCCGTTCTCGGTGGTTCCgggca                                                                     <  2:3240204/93‑3 (MQ=255)
                    tGAAAGGTGGCGATCCGTTTATTTTTGGCCGTGGTGGCGAAGAGCTGGAAACACTGTGCAACGCGGGTATCCCGTTCTCGGTGGTTCCgggca                                                                     >  2:2772956/1‑91 (MQ=255)
                     gAAAGGTGGCGATCCGTTTATTTTTGGCCGTGGTGGCGAAGAGCTGGAAACACTGTGCAACGCGGGTATCCCGTTCTCGGTGGTTCCGGGCAt                                                                    >  2:1864919/1‑93 (MQ=255)
                     gAAAGGTGGCGATCCGTTTATTTTTGGCCGTGGTGGCGAAGAGCTGGAAACACTGTGCAACGCGGGTATCCCGTTCTCGGTGGTTCCGGGCAt                                                                    >  1:3995696/1‑93 (MQ=255)
                     gAAAGGTGGCGATCCGTTTATTTTTGGCCGTGGTGGCGAAGAGCTGGAAACACTGTGCAACGCGGGTATCCCGTTCTCGGTGGTTCCGGGCAt                                                                    >  1:2210032/1‑93 (MQ=255)
                     gAAAGGTGGCGATCCGTTTATTTTTGGCCGTGGTGGCGAAGAGCTGGAAACACTGTGCAACGCGGGTATCCCGTTCTCGGTGGTTCCGGGCAt                                                                    >  1:4965171/1‑93 (MQ=255)
                      aaaGGTGGCGATCCGTTTATTTTTGGCCGTGGTGGCGAAGAGCTGGAAACACTGTGCAACGCGGGTATCCCGTTCTCGGTGGTTCCGGGCAtt                                                                   <  2:1770183/93‑1 (MQ=255)
                      aaaGGTGGCGATCAGTTTATTTTTGGCCGTGGTGGCGAAGAGCTGGAAACACTGTGCAACGCGGGTATCCCGTTCTCGGTGGTTCCGGGCAtt                                                                   >  1:836549/1‑93 (MQ=255)
                       aaGGTGGCGATCCGTTTATTTTTGGCCGTGGTGGCGAAGAGCTGGAAACACTGTGCAACGCGGGTATCCCGTTCTCGGTGGTTCCGGGCATTa                                                                  >  1:7765278/1‑93 (MQ=255)
                       aaGGTGGCGATCCGTTTATTTTTGGCCGTGGTGGCGAAGAGCTGGAAACACTGTGCAACGCGGGTATCCCGTTCTCGGTGGTTCCGGGCATTa                                                                  >  2:6656031/1‑93 (MQ=255)
                        aGGTGGCGATCCGTTTATTTTTGGCCGTGGTGGCGAAGAGCTGGAAACACTGTGCAACGCGGGTATCCCGTTCTCGGTGGTTCCGGGCATTAc                                                                 >  2:1099000/1‑93 (MQ=255)
                        aGGTGGCGATCCGTTTATTTTTGGCCGTGGTGGCGAAGAGCTGGAAACACTGTGCAACGCGGGTATCCCGTTCTCGGTGGTTCCGGGCATTAc                                                                 >  2:4813761/1‑93 (MQ=255)
                         ggTGGCGATCCGTTTATTTTTGGCCGTGGTGGCGAAGAGCTGGAAACACTGTGCAACGCGGGTATCCCGTTCTCGGTGGTTCCGGGCATTAcc                                                                <  2:135444/93‑1 (MQ=255)
                          gTGGAGATCCGTTTATTTTTGGCCGTGGTGGCGAAGAGCTGGAAACACTGTGCAACGCGGGTATCCCGTTCTCGGTGGTTCCGGGCATTACCg                                                               >  1:7836703/1‑93 (MQ=255)
                            ggCGATCCGTTTATTTTTGGCCGTGGTGGCGAAGAGCTGGAAACACTGTGCAACGCGGGAATCCCGTTCTCGGTGGTTCCGGGCATTACCGCa                                                             >  1:3491079/1‑93 (MQ=255)
                              cGATCCGTTTATTTTTGGCCGTGGTGGCGAAGAGCTGGAAACACTGTGCAACGCGGGTATCCCGTTCTCGGTGGTTCCGGGCATTACCGCAGc                                                           <  2:4182713/93‑1 (MQ=255)
                               gATCCGTTTATTTTTGGCCGTGGTGGCGAAGAGCTGGAAACACTGTGCAACGCGGGTATCCCGTTCTCGGTGGTTCCGGGCATTACCGCAGCt                                                          >  2:5514193/1‑93 (MQ=255)
                                 tCCGTTTATTTTTGGCCGTGGTGGCGAAGAGCTGGAAACACTGTGCAACGCGGGTATCCCGTTCTCGGTGGTTCCGGGCATTACCGCAGCTTc                                                        >  1:2802279/1‑93 (MQ=255)
                                 tCCGTTTATTTTTGGCCGTGGTGGCGAAGAGCTGGAAACACTGTGCAACGCGGGTATCCCGTTCTCGGTGGTTCCGGGCATTACCGCAGCTTc                                                        <  2:4887677/93‑1 (MQ=255)
                                 tCCGTTTATTTTTGGCCGTGGTGGCGAAGAGCTGGAAACACTGTGCAACGCGGGTATCCCGTTCTCGGTGGTTCCGGGCATTACCGCAGCTTc                                                        >  2:2261087/1‑93 (MQ=255)
                                   cGTTTATTTTTGGCCGTGGTGGCGAAGAGCTGGAAACACTGTGCAACGCGGGTATCCCGTTCTCGGTGGTTCCGGGCATTACCGCAGCTTCTg                                                      <  2:3485817/93‑1 (MQ=255)
                                    gTTTATTTTTGGCCGTGGTGGCGAAGAGCTGGAAACACTGTGCAACGCGGGTATCCCGTTCTCGGTGGTTCCGGGCATTACCGCAGCTTCTgg                                                     <  2:7070675/93‑1 (MQ=255)
                                     tttATTTTTGGCCGTGGTGGCGAAGAGCTGGAAACACTGTGCAACGCGGGTATCCCGTTCTCGGTGGTTCCGGGCATTACCGCAGCTTCTGGt                                                    <  1:6734428/93‑1 (MQ=255)
                                     tttATTTTTGGCCGTGGTGGCGAAGAGCTGGAAACACTGTGCAACGCGGGTATCCCGTTCTCGGTGGTTCCGGGCATTACCGCAGCTTCTGGt                                                    <  1:7253200/93‑1 (MQ=255)
                                     tttATTTTTGGCCGTGGTGGCGAAGAGCTGGAAACACTGTGCAACGCGGGTATCCCGTTCTCGGTGGTTCCGGGCATTACCGCAGCTTCTGGt                                                    <  1:6196934/93‑1 (MQ=255)
                                     tttATTTTTGGCCGTGGTGGCGAAGAGCTGGAAACACTGTGCAACGCGGGTATCCCGTTCTCGGTGGTTCCGGGCATTACCGCAGCTTCTGGt                                                    >  2:5114797/1‑93 (MQ=255)
                                     tttATTTTTGGCCGTGGTGGCGAAGAGCTGGAAACACTGTGCAACGCGGGTATCCCGTTCTCGGTGGTTCCGGGCATTACCGCAGCTTCTGGt                                                    >  1:4396434/1‑93 (MQ=255)
                                      ttATTTTTGGCCGTGGTGGCGAAGAGCTGGAAACACTGTGCAACGCGGGTATCCCGTTCTCGGTGGTTCCGGGCATTACCGCAGCTTCTGGtt                                                   >  2:5695852/1‑93 (MQ=255)
                                      ttATTTTTGGCCGTGGTGGCGAAGAGCTGGAAACACTGTGCAACGCGGGTATCCCGTTCTCGGTGGTTCCGGGCATTACCGCAGCTTCTGGtt                                                   <  1:3622699/93‑1 (MQ=255)
                                      ttATTTTTGGCCGAGGTGGCGAAGAGCTGGAAACACTGTGCAACGCGGGTATCCCGTTCTCGGTGGTTCCGGGCATTACCGCAGCGTCGGGtt                                                   >  2:1486234/1‑93 (MQ=255)
                                        aTTTTTGGCCGTGGTGGCGAAGAGCTGGAAACACTGTGCAACGCGGGTATCCCGTTCTCGGTGGTTCCGGGCATTACCGCAGCTTCTGGTTGc                                                 <  2:4379087/93‑1 (MQ=255)
                                        aTTTTTGGCCGTGGTGGCGAAGAGCTGGAAACACTGTGCAACGCGGGTATCCCGTTCTCGGTGGTTCCGGGCATTACCGCAGCTTCTGGTTGc                                                 <  2:6978913/93‑1 (MQ=255)
                                        aTTTTTGGCCGTGGTGGCGAAGAGCTGGAAACACTGTGCAACGCGGGTATCCCGTTCTCGGTGGTTCCGGGCATTACCGCAGCTTCTGGTTGc                                                 <  1:1322915/93‑1 (MQ=255)
                                          ttttGGCCGTGGTGGCGAAGAGCTGGAAACACTGTGCAACGCGGGTATCCCGTTCTCGGTGGTTCCGGGCATTACCGCAGCTTCTGGTTGctc                                               >  1:7549166/1‑93 (MQ=255)
                                          ttttGGCCGTGGTGGCGAAGAGCTGGAAACACTGTGCAACGCGGGTATCCCGTTCTCGGTGGTTCCGGGCATTACCGCAGCTTCTGGTTGctc                                               <  1:1599765/93‑1 (MQ=255)
                                              ggCCGTGGTGGCGAAGAGCTGGAAACACTGTGCAACGCGGGTATCCCGTTCTCGGTGGTTCCGGGCATTACCGCAGCTTCTGGTTGCTCTGcc                                           <  1:1129274/93‑1 (MQ=255)
                                                 cGTGGTGGCGAAGAGCTGGAAACACTGTGCAACGCGGGTATCCCGTTCTCGGTGGTTCCGGGCATTACCGCAGCTTCTGGTTGCTCTGCCTAt                                        <  2:4484362/93‑1 (MQ=255)
                                                  gtggtgGCGAAGAGCTGGAAACACTGTGCAACGCGGGTATCCCGTTCTCGGTGGTTCCGGGCATTACCGCAGCTTCTGGTTGCTCTGCCTAtt                                       >  2:5339349/1‑93 (MQ=255)
                                                  gtggtgGCGAAGAGCTGGAAACACTGTGCAACGCGGGTATCCCGTTCTCGGTGGTTCCGGGCATTACCGCAGCTTCTGGTTGCTCTGCCTAtt                                       >  2:6324226/1‑93 (MQ=255)
                                                  gtggtgGCGAAGAGCTGGAAACACTGTGCAACGCGGGTATCCCGTTCTCGGTGGTTCCGGGCATTACCGCAGCTTCTGGTTGCTCTGCCTAtt                                       <  1:3548998/93‑1 (MQ=255)
                                                   tggtggCGAAGAGCTGGAAACACTGTGCAACGCGGGTATCCCGTTCTCGGTGGTTCCGGGCATTACCGCAGCTTCTGGTTGCTCTGCCTATTc                                      >  1:1006254/1‑93 (MQ=255)
                                                   tggtggCGAAGAGCTGGAAACACTGTGCAACGCGGGTATCCCGTTCTCGGTGGTTCCGGGCATTACCGCAGCTTCTGGTTGCTCTGCCTATTc                                      <  1:6688576/93‑1 (MQ=255)
                                                    ggtggCGAAGAGCTGGAAACACTGTGCAACGCGGGTATCCCGTTCTCGGTGGTTCCGGGCATTACCGCAGCTTCTGGTTGCTCTGCCTATTCg                                     <  2:4660868/93‑1 (MQ=255)
                                                     gtggCGAAGAGCTGGAAACACTGTGCAACGCGGGTATCCCGTTCTCGGTGGTTCCGGGCATTACCGCAGCTTCTGGTTGCTCTGCCTATTCgg                                    <  2:1985701/93‑1 (MQ=255)
                                                      tggCGAAGAGCTGGAAACACTGTGCAACGCGGGTATCCCGTTCTCGGTGGTTCCGGGCATTACCGCAGCTTCTGGTTGCTCTGCCTATTCggg                                   >  2:2585850/1‑93 (MQ=255)
                                                      tggCGAAGAGCTGGAAACACTGTGCAACGCGGGTATCCCGTTCTCGGTGGTTCCGGGCATTACCGCAGCTTCTGGTTGCTCTGCCTATTCggg                                   >  1:5863248/1‑93 (MQ=255)
                                                         cGAAGAGCTGGAAACACTGTGCAACGCGGGTATCCCGTTCTCGGTGGTTCCGGGCATTACCGCAGCTTCTGGTTGCTCTGCCTATTCGGGTAt                                <  1:5305275/93‑1 (MQ=255)
                                                         cGAAGAGCTGGAAACACTGTGCAACGCGGGTATCCCGTTCTCGGTGGTTCCGGGCATTACCGCAGCTTCTGGTTGCTCTGCCTATTCGGGTAt                                <  2:2154897/93‑1 (MQ=255)
                                                         cGAAGAGCTGGAAACACTGTGCAACGCGGGTATCCCGTTCTCGGTGGTTCCGGGCATTACCGCAGCTTCTGGTTGCTCTGCCTATTCGGGTAt                                <  1:6942000/93‑1 (MQ=255)
                                                          gAAGAGCTGGAAACACTGTGCAACGCGGGTATCCCGTTCTCGGTGGTTCCGGGCATTACCGCAGCTTCTGGTTGCTCTGCCTATTCGGGTAtt                               <  1:6346377/93‑1 (MQ=255)
                                                           aaGAGCTGGAAACACTGTGCAACGCGGGTATCCCGTTCTCGGTGGTTCCGGGCATTACCGCAGCTTCTGGTTGCTCTGCCTATTCGGGTATTc                              >  1:3256571/1‑93 (MQ=255)
                                                            agagCTGGAAACACTGTGCAACGCGGGTATCCCGTTCTCGGTGGTTCCGGGCATTACCGCAGCTTCTGGTTGCTCTGCCTATTCGGGTATTcc                             >  2:4719484/1‑93 (MQ=255)
                                                            agagCTGGAAACACTGTGCAACGCGGGTATCCCGTTCTCGGTGGTTCCGGGCATTACCGCAGCTTCTGGTTGCTCTGCCTATTCGGGTATTcc                             >  2:860127/1‑93 (MQ=255)
                                                            agagCTGGAAACACTGTGCAACGCGGGTATCCCGTTCTCGGTGGTTCCGGGCATTACCGCAGCTTCTGGTTGCTCTGCCTATTCGGGTATTcc                             <  2:3625018/93‑1 (MQ=255)
                                                            agagCTGGAAACACTGTGCAACGCCGGTATCCCGTTCTCGGTGGTTCCGGGCATTACCGCAGCTTCTGGTTGCTCTGCCTATTCGGGTATTcc                             <  1:6278588/93‑1 (MQ=255)
                                                              agCTGGAAACACTGTGCAACGCGGGTATCCCGTTCTCGGTGGTTCCGGGCATTACCGCAGCTTCTGGTTGCTCTGCCTATTCGGGTATTCCAc                           <  1:6826072/93‑1 (MQ=255)
                                                                  ggAAACACTGTGCAACGCGGGTATCCCGTTCTCGGTGGTTCCGGGCATTACCGCAGCTTCTGGTTGCTCTGCCTATTCGGGTATTCCACTCAc                       <  2:7905279/93‑1 (MQ=255)
                                                                  ggAAACACTGTGCAACGCGGGTATCCCGTTCTCGGTGGTTCCGGGCATTACCGCAGCTTCTGGTTGCTCTGCCTATTCGGGTATTCCACTCAc                       >  1:4768415/1‑93 (MQ=255)
                                                                   gAAACACTGTGCAACGCGGGTATCTCGTTCTCGGTGGTTCCGGGCATTACCGCAGCTTCTGGTTGCTCTGCCTATTCGGGTATTCCACTCACg                      >  2:3348686/1‑93 (MQ=255)
                                                                   gAAACACTGTGCAACGCGGGTATCCCGTTCTCGGTGGTTCCGGGCATTACCGCAGCTTCTGGTTGCTCTGCCTATTCGGGTATTCCACTCACg                      <  2:5018670/93‑1 (MQ=255)
                                                                    aaaCACTGTGCAACGCGGGTATCCCGTTCTCGGTGGTTCCGGGCATTACCGCAGCTTCTGGTTGCTCTGCCTATTCGGGTATTCCACTCACGc                     <  2:1449414/93‑1 (MQ=255)
                                                                     aaCACTGTGCAACGCGGGTATCCCGTTCTCGGTGGTTCCGGGCATTACCGCAGCTTCTGGTTGCTCTGCCTATTCGGGTATTCCACTCACGCa                    >  1:2444449/1‑93 (MQ=255)
                                                                     aaCACTGTGCAACGCGGGTATCCCGTTCTCGGTGGTTCCGGGCATTACCGCAGCTTCTGGTTGCTCTGCCTATTCGGGTATTCCACTCACGCa                    >  1:6439797/1‑93 (MQ=255)
                                                                      acacTGTGCAACGCGGGTATCCCGTTCTCGGTGGTTCCGGGCATTACCGCAGCTTCTGGTTGCTCTGCCTATTCGGGTATTCCACTCACGCAt                   <  1:695550/93‑1 (MQ=255)
                                                                       cacTGTGCAACGCGGGTATCCCGTTCTCGGTGGTTCCGGGCATTACCGCAGCTTCTGGTTGCTCTGCCTATTCGGGTATTCCACTCACGCATc                  >  2:2774301/1‑93 (MQ=255)
                                                                       cacTGTGCAACGCGGGTATCCCGTTCTCGGTGGTTCCGGGCATTACCGCAGCTTCTGGTTGCTCTGCCTATTCGGGTATTCCACTCACGCATc                  <  2:5764207/93‑1 (MQ=255)
                                                                       cacTGTGCAACGCAGGTATCCCGTTCTCGGTGGTTCCGGGCATTACCGCAGCTTCTGGTTGCTCTGCCTATTCGGGTATTCCACTCACGCATc                  <  1:6993060/93‑1 (MQ=255)
                                                                        acTGTGCAACGCGGGTATCCCGTTCTCGGTGGTTCCGGGCATTACCGCAGCTTCTGGTTGCTCTGCCTATTCGGGTATTCCACTCACGCATcg                 <  2:7651071/93‑1 (MQ=255)
                                                                              cAACGCTGGTATCCCGTTCTCGGTGGTTCCGGGCATTACCGCAGCTTCTGGTTGCTCTGCCTATTCGGGTATTCCACTCACGCATCGCGATTa           <  1:197015/93‑1 (MQ=255)
                                                                              cAACGCGGGTATCCGGTTCTCGGTGTGTCCGGGCATTACCGCAGCTTCTGGTTGCTCTGCCTATTCGGGTATTCCACTCACGCATCGCGATTa           <  2:4216896/93‑1 (MQ=255)
                                                                              cAACGCGGGTATCCCGTTCTCGGTGGTTCCGGGCATTACCGCAGCTTCTGGTTGCTCTGCCTATTCGGGTATTCCACTCACGCATCGCGATTa           <  2:3163916/93‑1 (MQ=255)
                                                                              cAACGCGGGTATCCCGTTCTCGGTGGTTCCGGGCATTACCGCAGCTTCTGGTTGCTCTGCCTATTCGGGTATTCCACTCACGCATCGCGATTa           <  2:3839649/93‑1 (MQ=255)
                                                                               aaCGCGGGTATCCCGTTCTCGGTGGTTCCGGGCATTACCGCAGCTTCTGGTTGCTCTGCCTATTCGGGTATTCCACTCACGCATCGCGATTAt          <  2:1670303/93‑1 (MQ=255)
                                                                                aCGCCGGTATCCCGGTCTCGGTGGGTCCGGGCATTACCGCAGCTTCTGGTTGCTCTGCCTATTCGGGGATTCCACTCACGCAGCGCGATTATg         <  2:4516952/93‑1 (MQ=255)
                                                                                 cgcgGGTATCCCGTTCTCGGTGGTTCCGGGCATTACCGCAGCTTCTGGTTGCTCTGCCTATTCGGGTATTCCACTCACGCATCGCGATTATGc        <  2:4627722/93‑1 (MQ=255)
                                                                                   cgGGTATCCCGTTCTCGGTGGTTCCGGGCATTACCGCAGCTTCTGGTTGCTCTGCCTATTCGGGTATTCCACTCACGCATCGCGATTATGccc      <  2:2409757/93‑1 (MQ=255)
                                                                                    gggTATCCCGTTCTCGGTGGTTCCGGGCATTACCGCAGCTTCTGGTTGCTCTGCCTATTCGGGTATTCCACTCACGCATCGCGATTATGCCCa     <  1:427924/93‑1 (MQ=255)
                                                                                    gggTATCCCGTTCTCGGTGGTTCCGGGCATTACCGCAGCTTCTGGTTGCTCTGCCTATTCGGGTATTCCACTCACGCATCGCGATTATGCCCa     <  2:6350319/93‑1 (MQ=255)
                                                                                      gTATCCCGTTCTCGGTGGTTCCGGGCATTACCGCAGCTTCTGGTTGCTCTGCCTATTCGGGTATTCCACTCACGCATCGCGATTATGCCCaga   >  1:4551304/1‑93 (MQ=255)
                                                                                      gTATCCCGTTCTCGGTGGTTCCGGGCATTACCGCAGCTTCTGGTTGCTCTGCCTATTCGGGTATTCCACTCACGCATCGCGATTATGCCCaga   >  1:5469130/1‑93 (MQ=255)
                                                                                      gTATCCCGTTCTCGGTGGTTCCGGGCATTACCGCAGCTTCTGGTTGCTCTGCCTATTCGGGTACTCCACTCACGCATCGCGATTATGCCCaga   <  1:6508614/93‑1 (MQ=255)
                                                                                      gTATCCCGTTCTCGGTGGTTCCGGGCATTACCGCAGCTTCTGGTTGATCTGCCTATTCGGGTATTCCACTCACGCATCGCGATTATGCCCaga   >  2:7575759/1‑93 (MQ=255)
                                                                                       tATCCCGTTCTCGGTGGTTCCGGGCATTACCGCAGCTTCTGGTTGCTCTGCCTATTCGGGTATTCCACTCACGCATCGCGATTATGCCCagag  <  1:4700695/93‑1 (MQ=255)
                                                                                       tATCCCGTTCTCGGTGGTTCCGGGCATTACCGCAGCTTCTGGTTGCTCTGCCTATTCGGGTATTCCACTCACGCATCGCGATTATGCCCagag  <  2:2607144/93‑1 (MQ=255)
                                                                                       tATCCCGTTCTCGGTGGTTCCGGGCATTACCGCAGCTTCTGGTTGCTCTGCCTATTCGGGTATTCCACTCACGCATCGCGATTATGCCCagag  <  2:7336034/93‑1 (MQ=255)
                                                                                          |                                                                                         
AGGCAAACGCGTGGTGCGGCTGAAAGGTGGCGATCCGTTTATTTTTGGCCGTGGTGGCGAAGAGCTGGAAACACTGTGCAACGCGGGTATTCCGTTCTCGGTGGTTCCGGGTATTACCGCAGCTTCTGGTTGCTCTGCCTATTCGGGTATTCCACTCACGCATCGCGATTATGCCCAGAG  >  NC_000913/3496340‑3496519

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
AAAAGGCAAACGCGTGGTGCGGCTGAAAGGTGGCGATCCGTTTATTTTTGGCCGTGGTGGCGAAGAGCTGGAAACACTGTGCAACGCGGGTATTCCGTTCTCGGTGGTTCCGGGTATTACCGCAGCTTCTGGTTGCTCTGCCTATTCGGGTATTCCACTCACGCATCGCGATTATGCCCAGAGCGTA  >  NC_000913/3496337‑3496523
                                                                                             |                                                                                             
AAAAGGCAAACGCGTGGTGCGGCTGAAAGGTGGCGATCCGTTTATTTTTGGCCGTGGTGGCGAAGAGCTGGAAACACTGTGCAACGCGGGTATC                                                                                               <  SRR4302543.3398137/94‑1 (MQ=60)
  AAGGCAAACGCGTGGTGCGGCTGAAAGGTGGCGATCCGTTTATTTTTGGCCGTGGTGGCGAAGAGCTGGAAACACTGTGCAACGCGGGTATCCC                                                                                             <  SRR4302543.2393928/94‑1 (MQ=60)
  AAGGCAAACGCGTGGTGCGGCTGAAAGGTGGCGATCCGTTTATTTTTGGCCGTGGTGGCGAAGAGCTGGAAACACTGTGCAACGCGGGTATCCC                                                                                             >  SRR4302543.591034/1‑94 (MQ=60)
  AAGGCAAACGCGTGGTGCGGCTGAAAGGTGGCGATCCGTTTATTTTTGGCCGTGGTGGCGAAGAGCTGGAAACACTGTGCAACGCGGGTATCCC                                                                                             >  SRR4302543.591036/1‑94 (MQ=60)
      CAAACGCGTGGTGCGGCTGAAAGGTGGCGATCCGTTTATTTTTGGCCGTGGTGGCGAAGAGCTGGAAACACTGTGCAACGCGGGTATCCCGTTC                                                                                         >  SRR4302543.5953061/1‑94 (MQ=60)
       AAACGCGTGGTGCGGCTGAAAGGTGGCGATCCGTTTATTTTTGGCCGTGGTGGCGAAGAGCTGGAAACACTGTGCAACGCGGGTATCCCGTTCT                                                                                        >  SRR4302543.7926490/1‑94 (MQ=60)
       AAACGCGTGGTGCGGCTGAAAGGTGGCGATCCGTTTATTTTTGGCCGTGGTGGCGAAGAGCTGGAAACACTGTGCAACGCGGGTATCCCGTTCT                                                                                        >  SRR4302543.4481953/1‑94 (MQ=60)
       AAACGCGTGGTGCGGCTGAAAGGTGGCGATCCGTTTATTTTTGGCCGTGGTGGCGAAGAGCTGGAAACACTGTGCAACGCGGGTATCCCGTTCT                                                                                        <  SRR4302543.5203943/94‑1 (MQ=60)
        aactcgggtggctgcctaaagggggctgTCCGTGTATTTTTGGCCGTGGTGGCGAAGAGCTGGAAACACTGTGCAACGCGGGTATCCCGTTCTC                                                                                       <  SRR4302543.1806740/66‑1 (MQ=60)
        AACGCGTGGTGCGGCTGAAAGGTGGCGATCCGTTTATTTTTGGCCGTGGTGGCGAAGAGCTGGAAACACTGTGCAACGCGGGTATCCCGTTCTC                                                                                       <  SRR4302543.1806739/94‑1 (MQ=60)
        AACGCGTGGTGCGGCTGAAAGGTGGCGATCCGTTTATTTTTGGCCGTGGTGGCGAAGAGCTGGAAACACTGTGCAACGCGGGTATCCCGTTCTC                                                                                       >  SRR4302543.6102669/1‑94 (MQ=60)
         ACGCGTGGTGCGGCTGAAAGGTGGCGATCCGTTTATTTTTGGCCGTGGTGGCGAAGAGCTGGAAACACTGTGCAACGCGGGTATCCCGTTCTCG                                                                                      <  SRR4302543.6136638/94‑1 (MQ=60)
          CGCGTGGTGCGGCTGAAAGGTGGCGATCCGTTTATTTTTGGCCGTGGTGGCGAAGAGCTGGAAACACTGTGCAACGCGGGTATCCCGTTCTCGG                                                                                     >  SRR4302543.1453315/1‑94 (MQ=60)
             GTGGTGCGGCTGAAAGGTGGCGATCCGTTTATTTTTGGCCGTGGTGGCGAAGAGCTGGAAACACTGTGCAACGCGGGTATCCCGTTCTCGGTGG                                                                                  >  SRR4302543.945509/1‑94 (MQ=60)
               GGTGCGGCTGAAAGGTGGCGATCCGTTTATTTTTGGCCGTGGTGGCGAAGAGCTGGAAACACTGTGCAACGCGGGTATCCCGTTCTCGGTGGTT                                                                                <  SRR4302543.7495443/94‑1 (MQ=60)
               GGTGCGGCTGAAAGGTGGCGATCCGTTTATTTTTGGCCGTGGTGGCGAAGAGCTGGAAACACTGTGCAACGCGGGTATCCCGTTCTCGGTGGTT                                                                                <  SRR4302543.1601471/94‑1 (MQ=60)
               GGTGCGGCTGAAAGGTGGCGATCCGTTTATTTTTGGCCGTGGTGGCGAAGAGCTGGAAACACTGTGCAACGCGGGTATCCCGTTCTCGGTGGTT                                                                                >  SRR4302543.4602784/1‑94 (MQ=60)
               GGTGCGGCTGAAAGGTGGCGATCCGTTTATTTTTGGCCGTGGTGGCGAAGAGCTGGAAACACTGTGCAACGCGGGTATCCCGTTCTCGGTGGTT                                                                                >  SRR4302543.5507040/1‑94 (MQ=60)
               GGTGCGGCTGAAACGTGGCGATCCGTTTATTTTTGGCCGTGGTGGCGAAGAGCTGGAAACACTGTGCAACGCGGGTATCCCGTTCTCGGTGGTT                                                                                <  SRR4302543.532470/94‑1 (MQ=60)
                GTGCGGCTGAAAGGTGGCGATCCGTTTATTTTTGGCCGTGGTGGCGAAGAGCTGGAAACACTGTGCAACGCGGGTATCCCGTTCTCGGTGGTTC                                                                               >  SRR4302543.4504159/1‑94 (MQ=60)
                GTGCGGCTGAAAGGTGGCGATCCGTTTATTTTTGGCCGTGGTGGCGAAGAGCTGGAAACACTGTGCAACGCGGGTATCCCGTTCTCGGTGGTTC                                                                               <  SRR4302543.3572880/94‑1 (MQ=60)
                 TGCGGCTGAAAGGTGGCGATCCGTTTATTTTTGGCCGTGGTGGCGAAGAGCTGGAAACACTGTGCAACGCGGGTATCCCGTTCTCGGTGGTTCC                                                                              <  SRR4302543.7630407/94‑1 (MQ=60)
                 TGCGGCTGAAAGGTGGCGATCCGTTTATTTTTGGCCGTGGTGGCGAAGAGCTGGAAACACTGTGCAACGCGGGTATCCCGTTCTCGGTGGTTCC                                                                              <  SRR4302543.7802143/94‑1 (MQ=60)
                   CGGCTGAAAGGTGGCGATCCGTTTATTTTTGGCCGTGGTGGCGAAGAGCTGGAAACACTGTGCAACGCGGGTATCCCGTTCTCGGTGGTTCCGG                                                                            <  SRR4302543.7015432/94‑1 (MQ=60)
                    GGCTGAAAGGTGGCGATCCGTTTATTTTTGGCCGTGGTGGCGAAGAGCTGGAAACACTGTGCAACGCGGGTATCCCGTTCTCGGTGGTTCCGGG                                                                           <  SRR4302543.1710934/94‑1 (MQ=60)
                    GGCTGAAAGGTGGCGATCCGTTTATTTTTGGCCGTGGTGGCGAAGAGCTGGAAACACTGTGCAACGCGGGTATCCCGTTCTCGGTGGTTCCGGG                                                                           <  SRR4302543.4331936/94‑1 (MQ=60)
                     GCTGAAAGGTGGCGATCCGTTTATTTTTGGCCGTGGTGGCGAAGAGCTGGAAACACTGTGCAACGCGGGTATCCCGTTCTCGGTGGTTCCGGGC                                                                          <  SRR4302543.5753834/94‑1 (MQ=60)
                      CTGAAAGGTGGCGATCCGTTTATTTTTGGCCGTGGTGGCGAAGAGCTGGAAACACTGTGCAACGCGGGTATCCCGTTCTCGGTGGTTCCGGGCA                                                                         >  SRR4302543.6982967/1‑94 (MQ=60)
                       TGAAAGGTGGCGATCCGTTTATTTTTGGCCGTGGTGGCGAAGAGCTGGAAACACTGTGCAACGCGGGTATCCCGTTCTCGGTGGTTCCGGGCAT                                                                        <  SRR4302543.7463499/94‑1 (MQ=60)
                       TGAAAGGTGGCGATCCGTTTATTTTTGGCCGTGGTGGCGAAGAGCTGGAAACACTGTGCAACGCGGGTATCCCGTTCTCGGTGGTTCCGGGCAT                                                                        >  SRR4302543.5514793/1‑94 (MQ=60)
                       TGAAAGGTGGCGATCCGTTTATTTTTGGCCGTGGTGGCGAAGAGCTGGAAACACTGTGCAACGCGGGTATCCCGTTCTCGGTGGTTCCGGGCAT                                                                        >  SRR4302543.5010368/1‑94 (MQ=60)
                       TGAAAGGTGGCGATCCGTTTATTTTTGGCCGTGGTGGCGAAGAGCTGGAAACACTGTGCAACGCGGGTATCCCGTTCTCGGTGGTTCCGGGCAT                                                                        >  SRR4302543.4031300/1‑94 (MQ=60)
                       TGAAAGGTGGCGATCCGTTTATTTTTGGCCGTGGTGGCGAAGAGCTGGAAACACTGTGCAACGCGGGTATCCCGTTCTCGGTGGTTCCGGGCAT                                                                        >  SRR4302543.2229939/1‑94 (MQ=60)
                        GAAAGGTGGCGATCAGTTTATTTTTGGCCGTGGTGGCGAAGAGCTGGAAACACTGTGCAACGCGGGTATCCCGTTCTCGGTGGTTCCGGGCATT                                                                       >  SRR4302543.843932/1‑94 (MQ=60)
                         AAAGGTGGCGATCCGTTTATTTTTGGCCGTGGTGGCGAAGAGCTGGAAACACTGTGCAACGCGGGTATCCCGTTCTCGGTGGTTCCGGGCATTA                                                                      >  SRR4302543.7838555/1‑94 (MQ=60)
                           AGGTGGCGATCCGTTTATTTTTGGCCGTGGTGGCGAAGAGCTGGAAACACTGTGCAACGCGGGTATCCCGTTCTCGGTGGTTCCGGGCATTACC                                                                    >  SRR4302543.3272528/1‑94 (MQ=60)
                            GGTGGAGATCCGTTTATTTTTGGCCGTGGTGGCGAAGAGCTGGAAACACTGTGCAACGCGGGTATCCCGTTCTCGGTGGTTCCGGGCATTACCG                                                                   >  SRR4302543.7910466/1‑94 (MQ=60)
                              TGGCGATCCGTTTATTTTTGGCCGTGGTGGCGAAGAGCTGGAAACACTGTGCAACGCGGGAATCCCGTTCTCGGTGGTTCCGGGCATTACCGCA                                                                 >  SRR4302543.3522327/1‑94 (MQ=60)
                                GCGATCCGTTTATTTTTGGCCGTGGTGGCGAAGAGCTGGAAACACTGTGCAACGCGGGTATCCCGTTCTCGGTGGTTCCGGGCATTACCGCAGC                                                               <  SRR4302543.6429632/94‑1 (MQ=60)
                                 CGATCCGTTTATTTTTGGCCGTGGTGGCGAAGAGCTGGAAACACTGTGCAACGCGGGTATCCCGTTCTCGGTGGTTCCGGGCATTACCGCAGCT                                                              <  SRR4302543.6328388/94‑1 (MQ=60)
                                  GATCCGTTTATTTTTGGCCGTGGTGGCGAAGAGCTGGAAACACTGTGCAACGCGGGTATCCCGTTCTCGGTGGTTCCGGGCATTACCGCAGCTT                                                             >  SRR4302543.5436915/1‑94 (MQ=60)
                                  GATCCGTTTATTTTTGGCCGTGGTGGCGAAGAGCTGGAAACACTGTGCAACGCGGGTATCCCGTTCTCGGTGGTTCCGGGCATTACCGCAGCTT                                                             >  SRR4302543.4364652/1‑94 (MQ=60)
                                  GATCCGTTTATTTTTGGCCGTGGTGGCGAAGAGCTGGAAACACTGTGCAACGCGGGTATCCCGTTCTCGGTGGTTCCGGGCATTACCGCAGCTT                                                             >  SRR4302543.72875/1‑94 (MQ=60)
                                   ATCCGTTTATTTTTGGCCGTGGTGGCGAAGAGCTGGAAACACTGTGCAACGCGGGTATCCCGTTCTCGGTGGTTCCGGGCATTACCGCAGCTTC                                                            >  SRR4302543.2827905/1‑94 (MQ=60)
                                    TCCGTTTATTTTTGGCCGTGGTGGCGAAGAGCTGGAAACACTGTGCAACGCGGGTATCCCGTTCTCGGTGGTTCCTGGCATTACCGCAGCTTCT                                                           >  SRR4302543.5592293/1‑94 (MQ=60)
                                    TCCGTTTATTTTTGGCCGTGGTGGCGAAGAGCTGGAAACACTGTGCAACGCGGGTATCCCGTTCTCGGTGGTTCCGGGCATTACCGCAGCTTCT                                                           <  SRR4302543.2854993/94‑1 (MQ=60)
                                    TCCGTTTATTTTTGGCCGTGGTGGCGAAGAGCTGGAAACACTGTGCAACGCGGGTATCCCGTTCTCGGTGGTTCCGGGCATTACCGCAGCTTCT                                                           <  SRR4302543.6659204/94‑1 (MQ=60)
                                     CCGTTTATTTTTGGCCGTGGTGGCGAAGAGCTGGAAACACTGTGCAACGCGGGTATCCCGTTCTCGGTGGTTCCGGGCATTACCGCAGCTTCTG                                                          >  SRR4302543.4866396/1‑94 (MQ=60)
                                     CCGTTTATTTTTGGCCGTGGTGGCGAAGAGCTGGAAACACTGTGCAACGCGGGTATCCCGTTCTCGGTGGTTCCGGGCATTACCGCAGCTTCTG                                                          <  SRR4302543.6783202/94‑1 (MQ=60)
                                     CCGTTTATTTTTGGCCGTGGTGGCGAAGAGCTGGAAACACTGTGCAACGCGGGTATCCCGTTCTCGGTGGTTCCGGGCATTACCGCAGCTTCTG                                                          <  SRR4302543.825890/94‑1 (MQ=60)
                                      CGTTTATTTTTGGCCGTGGTGGCGAAGAGCTGGAAACACTGTGCAACGCGGGTATCCCGTTCTCGGTGGTTCCGGGCATTACCGCAGCTTCTGG                                                         <  SRR4302543.3571953/94‑1 (MQ=60)
                                       GTTTATTTTTGGCCGTGGTGGCTAAGAGCTGGAAACACTGTGCAACGCGGGTATCCCGTTCTCGGTGGTTCCGGGCATTACCGCAGCTTCTGGT                                                        >  SRR4302543.1391166/1‑94 (MQ=60)
                                       GTTTATTTTTGGCCGTGGTGGCGAAGAGCTGGAAACACTGTGCAACGCGGGTATCCCGTTCTCGGTGGTTCCGGGCATTACCGCAGCTTCTGGT                                                        >  SRR4302543.4768600/1‑94 (MQ=60)
                                       GTTTATTTTTGGCCGTGGTGGCGAAGAGCTGGAAACACTGTGCAACGCGGGTATCCCGTTCTCGGTGGTTCCGGGCATTACCGCAGCTTCTGGT                                                        <  SRR4302543.6413895/94‑1 (MQ=60)
                                       GTTTATTTTTGGCCGTGGTGGCGAAGAGCTGGAAACACTGTGCAACGCGGGTATCCCGTTCTCGGTGGTTCCGGGCATTACCGCAGCTTCTGGT                                                        >  SRR4302543.4436290/1‑94 (MQ=60)
                                        TTTATTTTTGGCCGTGGTGGCGAAGAGCTGGAAACACTGTGCAACGCGGGTATCCCGTTCTCGGTGGTTCCGGGCATTACCGCAGCTTCTGGTT                                                       <  SRR4302543.7323332/94‑1 (MQ=60)
                                        TTTATTTTTGGCCGTGGTGGCGAAGAGCTGGAAACACTGTGCAACGCGGGTATCCCGTTCTCGGTGGTTCCGGGCATTACCGCAGCTTCTGGTT                                                       <  SRR4302543.6255466/94‑1 (MQ=60)
                                        TTTATTTTTGGCCGTGGTGGCGAAGAGCTGGAAACACTGTGCAACGCGGGTATCCCGTTCTCGGTGGTTCCGGGCATTACCGCAGCTTCTGGTT                                                       <  SRR4302543.3639083/94‑1 (MQ=60)
                                        TTTATTTTTGGCCGTGGTGGCGAAGAGCTGGAAACACTGTGCAACGCGGGTATCCCGTTCTCGGTGGTTCCGGGCATTACCGCAGCTTCTGGTT                                                       <  SRR4302543.6798049/94‑1 (MQ=60)
                                         TTATTTTTGGCCGTGGTGGCGAAGAGCTGGAAACACTGTGCAACGCGGGTATCCCGTTCTCGGTGGTTCCGGGCATTACCGCAGCTTCTGGTTG                                                      <  SRR4302543.3654620/94‑1 (MQ=60)
                                          TATTTTTGGCCGTGGTGGCGAAGAGCTGGAAACACTGTGCAACGCGGGTATCCCGTTCTCGGTGGTTCCGGGCATTACCGCAGCTTCTGGTTGC                                                     >  SRR4302543.4076054/1‑94 (MQ=60)
                                           ATTTTTGGCCGTGGTGGCGAAGAGCTGGAAACACTGTGCAACGCGGGTATCCCGTTCTCGGTGGTTCCGGGCATTACCGCAGCTTCTGGTTGCT                                                    <  SRR4302543.1334663/94‑1 (MQ=60)
                                           ATTTTTGGCCGTGGTGGCGAAGAGCTGGAAACACTGTGCAACGCGGGTATCCCGTTCTCGGTGGTTCCGGGCATTACCGCAGCTTCTGGTTGCT                                                    <  SRR4302543.688866/94‑1 (MQ=60)
                                            TTTTTGGCCGTGGTGGCGAAGAGCTGGAAACACTGTGCAACGCGGGTATCCCGTTCTCGGTGGTTCCGGGCATTACCGCAGCTTCTGGTTGCTC                                                   >  SRR4302543.7621077/1‑94 (MQ=60)
                                             TTTTGGCCGTGGTGGCGAAGAGCTGGAAACACTGTGCAACGCGGGTATCCCGTTCTCGGTGGTTCCGGGCATTACCGCAGCTTCTGGTTGCTCT                                                  <  SRR4302543.1614037/94‑1 (MQ=60)
                                              TTTGGCCGTGGTGGCGAAGAGCTGGAAACACTGTGCAACGCGGGTATCCCGTTCTCGGTGGTTCCGGGCATTACCGCAGCTTCTGGTTGCTCTG                                                 <  SRR4302543.3946850/94‑1 (MQ=60)
                                               TTGGCCGTGGTGGCGAAGAGCTGGAAACACTGTGCAACGCGGGTATCCCGTTCTCGGTGGTTCCGGGCATTACCGCAGCTTCTGGTTGCTCTGC                                                <  SRR4302543.1580879/94‑1 (MQ=60)
                                                 GGCCGTGGTGGCGAAGAGCTGGAAACACTGTGCAACGCGGGTATCCCGTTCTCGGTGGTTCCGGGCATTACCGCAGCTTCTGGTTGCTCTGCCT                                              <  SRR4302543.1139524/94‑1 (MQ=60)
                                                    CGTGGTGGCGAAGAGCTGGAAACACTGTGCAACGCGGGTATCCCGTTCTCGGTGGTTCCGGGCATTACCGCAGCTTCTGGTTGCTCTGCCTATT                                           >  SRR4302543.1329144/1‑94 (MQ=60)
                                                    CGTGGTGGCGAAGAGCTGGAAACACTGTGCAACGCGGGTATCCCGTTCTCGGTGGTTCCGGGCATTACCGCAGCTTCTGGTTGCTCTGCCTATT                                           <  SRR4302543.1674773/94‑1 (MQ=60)
                                                    CGTGGTGGCGAAGAGCTGGAAACACTGTGCAACGCGGGTATCCCGTTCTCGGTGGTTCCGGGCATTACCGCAGCTTCTGGTTGCTCTGCCTATT                                           <  SRR4302543.7355788/94‑1 (MQ=60)
                                                     GTGGTGGCGAAGAGCTGGAAACACTGTGCAACGCGGGTATCCCGTTCTCGGTGGTTCCGGGCATTACCGCAGCTTCTGGTTGCTCTGCCTATTC                                          <  SRR4302543.2139701/94‑1 (MQ=60)
                                                     GTGGTGGCGAAGAGCTGGAAACACTGTGCAACGCGGGTATCCCGTTCTCGGTGGTTCCGGGCATTACCGCAGCTTCTGGTTGCTCTGCCTATTC                                          >  SRR4302543.1015156/1‑94 (MQ=60)
                                                     GTGGTGGCGAAGAGCTGGAAACACTGTGCAACGCGGGTATCCCGTTCTCGGTGGTTCCGGGCATTACCGCAGCTTCTGGTTGCTCTGCCTATTC                                          >  SRR4302543.1015157/1‑94 (MQ=60)
                                                     GTGGTGGCGAAGAGCTGGAAACACTGTGCAACGCGGGTATCCCGTTCTCGGTGGTTCCGGGCATTACCGCAGCTTCTGGTTGCTCTGCCTATTC                                          <  SRR4302543.3580491/94‑1 (MQ=60)
                                                     GTGGTGGCGAAGAGCTGGAAACACTGTGCAACGCGGGTATCCCGTTCTCGGTGGTTCCGGGCATTACCGCAGCTTCTGGTTGCTCTGCCTATTC                                          <  SRR4302543.2538303/94‑1 (MQ=60)
                                                      TGGTGGCGAAGAGCTGGAAACACTGTGCAACGCGGGTATCCCGTTCTCGGTGGTTCCGGGCATTACCGCAGCTTCTGGTTGCTCTGCCTATTCG                                         <  SRR4302543.3357340/94‑1 (MQ=60)
                                                      TGGTGGCGAAGAGCTGGAAACACTGTGCAACGCGGGTATCCCGTTCTCGGTGGTTCCGGGCATTACCGCAGCTTCTGGTTGCTCTGCCTATTCG                                         <  SRR4302543.6751712/94‑1 (MQ=60)
                                                        GTGGCGAAGAGCTGGAAACCCTGTGCAACGCGGGTATCCCGTTCTCGGTGGTTCCGGGCATTACCGCAGCTTCTGGTTGCTCTGCCTATTCGGG                                       <  SRR4302543.3405667/94‑1 (MQ=60)
                                                        GTGGCGAAGAGCTGGAAACACTGTGCAACGCGGGTATCCCGTTCTCGGTGGTTCCGGGCATTACCGCAGCTTCTGGTTGCTCTGCCTATTCGGG                                       >  SRR4302543.5916614/1‑94 (MQ=60)
                                                        GTGGCGAAGAGCTGGAAACACTGTGCAACGCGGGTATCCCGTTCTCGGTGGTTCCGGGCATTACCGCAGCTTCTGGTTGCTCTGCCTATTCGGG                                       >  SRR4302543.5582729/1‑94 (MQ=60)
                                                          GGCGAAGAGCTGGAAACACTGTGCAACGCGGGTATCCCGTTCTCGGTGGTTCCGGGCATTACCGCAGCTTCTGGTTGCTCTGCCTATTCGGGTA                                     <  SRR4302543.5515187/94‑1 (MQ=60)
                                                           GCGAAGAGCTGGAAACACTGTGCAACGCGGGTATCCCGTTCTCGGTGGTTCCGGGCATTACCGCAGCTTCTGGTTGCTCTGCCTATTCGGGTAT                                    >  SRR4302543.140281/1‑94 (MQ=60)
                                                           GCGAAGAGCTGGAAACACTGTGCAACGCGGGTATCCCGTTCTCGGTGGTTCCGGGCATTACCGCAGCTTCTGGTTGCTCTGCCTATTCGGGTAT                                    <  SRR4302543.4444658/94‑1 (MQ=60)
                                                           GCGAAGAGCTGGAAACACTGTGCAACGCGGGTATCCCGTTCTCGGTGGTTCCGGGCATTACCGCAGCTTCTGGTTGCTCTGCCTATTCGGGTAT                                    <  SRR4302543.1024791/94‑1 (MQ=60)
                                                            CGAAGAGCTGGAAACACTGTGCAACGCGGGTATCCCGTTCTCGGTGGTTCCGGGCATTACCGCAGCTTCTGGTTGCTCTGCCTATTCGGGTATT                                   >  SRR4302543.6872616/1‑94 (MQ=60)
                                                            CGAAGAGCTGGAAACACTGTGCAACGCGGGTATCCCGTTCTCGGTGGTTCCGGGCATTACCGCAGCTTCTGGTTGCTCTGCCTATTCGGGTATT                                   <  SRR4302543.7007303/94‑1 (MQ=60)
                                                            CGAAGAGCTGGAAACACTGTGCAACGCGGGTATCCCGTTCTCGGTGGTTCCGGGCATTACCGCAGCTTCTGGTTGCTCTGCCTATTCGGGTATT                                   >  SRR4302543.4648732/1‑94 (MQ=60)
                                                            CGAAGAGCTGGAAACACTGTGCAACGCGGGTATCCCGTTCTCGGTGGTTCCGGGCATTACCGCAGCTTCTGGTTGCTCTGCCTATTCGGGTATT                                   <  SRR4302543.5353181/94‑1 (MQ=60)
                                                             GAAGAGCTGGAAACACTGTGCAACGCGGGTATCCCGTTCTCGGTGGTTCCGGGCATTACCGCAGCTTCTGGTTGCTCTGCCTATTCGGGTATTC                                  <  SRR4302543.6585689/94‑1 (MQ=60)
                                                             GAAGAGCTGGAAACACTGTGCAACGCGGGTATCCCGTTCTCGGTGGTTCCGGGCATTACCGCAGCTTCTGGTTGCTCTGCCTATTCGGGTATTC                                  <  SRR4302543.6406049/94‑1 (MQ=60)
                                                             GAAGAGCTGGAAACACTGTGCAACGCGGGTATCCCGTTCTCGGTGGTTCCGGGCATTACCGCAGCTTCTGGTTGCTCTGCCTATTCGGGTATTC                                  >  SRR4302543.3286009/1‑94 (MQ=60)
                                                              AAGAGCTGGAAACACTGTGCAACGCGGGTATCCCGTTCTCGGTGGTTCCGGGCATTACCGCAGCTTCTGGTTGCTCTGCCTATTCGGGTATTCC                                 <  SRR4302543.3677247/94‑1 (MQ=60)
                                                              AAGAGCTGGAAACACTGTGCAACGCGGGTATCCCGTTCTCGGTGGTTCCGGGCATTACCGCAGCTTCTGGTTGCTCTGCCTATTCGGGTATTCC                                 >  SRR4302543.7015598/1‑94 (MQ=60)
                                                               AGAGCTGGAAACACTGTGCAACGCGGGTATCCCGTTCTCGGTGGTTCCGGGCATTACCGCAGCTTCTGGTTGCTCTGCCTATTCGGGTATTCCA                                >  SRR4302543.259883/1‑94 (MQ=60)
                                                               AGAGCTGGAAACACTGTGCAACGCCGGTATCCCGTTCTCGGTGGTTCCGGGCATTACCGCAGCTTCTGGTTGCTCTGCCTATTCGGGTATTCCA                                <  SRR4302543.6337771/94‑1 (MQ=60)
                                                                 AGCTGGAAACACTGTGCAACGCGGGTATCCCGTTCTCGGTGGTTCCGGGCATTACCGCAGCTTCTGGTTGCTCTGCCTATTCGGGTATTCCACT                              <  SRR4302543.6890515/94‑1 (MQ=60)
                                                                  GCTGGAAACACTGTGCAACGCGGGTATCCCGTTCTCGGTGGTTCCTGGCATTACCGCAGCTTCTGGTTGCTCTGCCTATTCGGGTATTCCACTC                             >  SRR4302543.7050755/1‑94 (MQ=60)
                                                                   CTGGAAACACTGTGCAACGCGGGTATCCCGTTCTCGGTGGTTCCGGGCATTACCGCAGCTTCTGGTTGCTCTGCCTATTCGGGTATTCCACTCA                            >  SRR4302543.597311/1‑94 (MQ=60)
                                                                    TGGAAACACTGTGCAACGCGGGTATCCCGTTCTCGGTGGTTCCGGGCATTACCGCAGCTTCTGGTTGCTCTGCCTATTCGGGTATTCCACTCAC                           >  SRR4302543.4811998/1‑94 (MQ=60)
                                                                    TGGAAACACTGTGCAACGCGGGTATCCCGTTCTCGGTGGTTCCGGGCATTACCGCAGCTTCTGGTTGCTCTGCCTATTCGGGTATTCCACTCAC                           <  SRR4302543.4744634/94‑1 (MQ=60)
                                                                    TGGAAACACTGTGCAACGCGGGTATCCCGTTCTCGGTGGTTCCGGGCATTACCGCAGCTTCTGGTTGCTCTGCCTATTCGGGTATTCCACTCAC                           >  SRR4302543.7867311/1‑94 (MQ=60)
                                                                    TGGAAACACTGTGCAACGCGGGTATCCCGTTCTCGGTGGTTCCGGGCATTACCGCAGCTTCTGGTTGCTCTGCCTATTCGGGTATTCCACTCAC                           <  SRR4302543.5425970/94‑1 (MQ=60)
                                                                     GGAAACACTGTGCAACGCGGGTATCCCGTTCTCGGTGGTTCCGGGCATTACCGCAGCTTCTGGTTGCTCTGCCTATTCGGGTATTCCACTCACG                          >  SRR4302543.4942258/1‑94 (MQ=60)
                                                                      GAAACACTGTGCAACGCGGGTATCCCGTTCTCGGTGGTTCCGGGCATTACCGCAGCTTCTGGTTGCTCTGCCTATTCGGGTATTCCACTCACGC                         >  SRR4302543.1713415/1‑94 (MQ=60)
                                                                       AAACACTGTGCAACGCGGGTATCCCGTTCTCGGTGGTTCCGGGCATTACCGCAGCTTCTGGTTGCTCTGCCTATTCGGGTATTCCACTCACGCA                        >  SRR4302543.2466482/1‑94 (MQ=60)
                                                                       AAACACTGTGCAACGCGGGTATCCCGTTCTCGGTGGTTCCGGGCATTACCGCAGCTTCTGGTTGCTCTGCCTATTCGGGTATTCCACTCACGCA                        >  SRR4302543.7000409/1‑94 (MQ=60)
                                                                       AAACACTGTGCAACGCGGGTATCCCGTTCTCGGTGGTTCCGGGCATTACCGCAGCTTCTGGTTGCTCTGCCTATTCGGGTATTCCACTCACGCA                        >  SRR4302543.2352054/1‑94 (MQ=60)
                                                                       AAACACTGTGCAACGCGGGTATCCCGTTCTCGGTGGTTCCGGGCATTACCGCAGCTTCTGGTTGCTCTGCCTATTCGGGTATTCCACTCACGCA                        >  SRR4302543.6500470/1‑94 (MQ=60)
                                                                        AACACTGTGCAACGCGGGTATCCCGTTCTCGGTGGTTCCGGGCATTACCGCAGCTTCTGGTTGCTCTGCCTATTCGGGTATTCCACTCACGCAT                       >  SRR4302543.4572750/1‑94 (MQ=60)
                                                                        AACACTGTGCAACGCGGGTATCCCGTTCTCGGTGGTTCCGGGCATTACCGCAGCTTCTGGTTGCTCTGCCTATTCGGGTATTCCACTCACGCAT                       >  SRR4302543.941098/1‑94 (MQ=60)
                                                                         ACACTGTGCAACGCGGGTATCCCGTTCTCGGTGGTTCCGGGCATTACCGCAGCTTCTGGTTGCTCTGCCTATTCGGGTATTCCACTCACGCATC                      >  SRR4302543.6288954/1‑94 (MQ=60)
                                                                         ACACTGTGCAACGCGGGTATCCCGTTCTCGGTGGTTCCGGGCATTACCGCAGCTTCTGGTTGCTCTGCCTATTCGGGTATTCCACTCACGCATC                      <  SRR4302543.701924/94‑1 (MQ=60)
                                                                          CACTGTGCAACGCGGGTATTCCGTTCTCGGTGGTTCCGGGTATTACCGCAGCTTCTGGTTGCTCTGCCTATTCGGGTATTCCACTCACGCATCG                     <  SRR4302543.70567/94‑1 (MQ=60)
                                                                          CACTGTGCAACGCGGGTATCCCGTTCTCGGTGGTTCCGGGCATTACCGCAGCTTCTGGTTGCTCTGCCTATTCGGGTATTCCACTCATGCATCG                     >  SRR4302543.5212503/1‑94 (MQ=60)
                                                                          CACTGTGCAACGCGGGTATCCCGTTCTCGGTGGTTCCGGGCATTACCGCAGCTTCTGGTTGCTCTGCCTATTCGGGTATTCCACTCACCCATCG                     >  SRR4302543.6484589/1‑94 (MQ=60)
                                                                          CACTGTGCAACGCAGGTATCCCGTTCTCGGTGGTTCCGGGCATTACCGCAGCTTCTGGTTGCTCTGCCTATTCGGGTATTCCACTCACGCATCG                     <  SRR4302543.7058903/94‑1 (MQ=60)
                                                                           ACTGTGCAACGCGGGTATCCCGTTCTCGGTGGTTCCGGGCATTACCGCAGCTTCTGGTTGCTCTGCCTATTCGGGTATTCCACTCACGCATCGC                    >  SRR4302543.1860093/1‑94 (MQ=60)
                                                                            CTGTGCAACGCGGGTATCCCGTTCTCGGTGGTTCCGGGCATTACCGCAGCTTCTGGTTGCTCTGCCTATTCGGGTATTCCACTCACGCATCGCG                   <  SRR4302543.6846757/94‑1 (MQ=60)
                                                                            CTGTGCAACGCGGGTATCCCGTTCTCGGTGGTTCCGGGCATTACCGCAGCTTCTGGTTGCTCTGCCTATTCGGGTATTCCACTCACGCATCGCG                   <  SRR4302543.7889696/94‑1 (MQ=60)
                                                                             TGTGCAACGCGGGTATCCCGTTCTCGGTGGTTCCGGGCATTACCGCAGCTTCTGGTTGCTCTGCCTATTCGGGTATTCCACTCACGCATCGCGA                  >  SRR4302543.6007037/1‑94 (MQ=60)
                                                                               TGCAACGCGGGTATCCCGTTCTCGGTGGTTCCGGGCATTACCGCAGCTTCTGGTTGCTCTGCCTATTCGGGTATTCCACTCACGCATCGCGATT                >  SRR4302543.61832/1‑94 (MQ=60)
                                                                               TGCAACGCGGGTATCCCGTTCTCGGTGGTTCCGGGCATTACCGCAGCTTCTGGTTGCTCTGCCTATTCGGGTATTCCACTCACGCATCGCGATT                <  SRR4302543.2004450/94‑1 (MQ=60)
                                                                               TGCAACGCGGGTATCCCGTTCTCGGTGGTTCCGGGCATTACCGCAGCTTCTGGTTGCTCTGCCTATTCGGGTATTCCACTCACGCATCGCGATT                >  SRR4302543.6760125/1‑94 (MQ=60)
                                                                                 CAACGCTGGTATCCCGTTCTCGGTGGTTCCGGGCATTACCGCAGCTTCTGGTTGCTCTGCCTATTCGGGTATTCCACTCACGCATCGCGATTAT              <  SRR4302543.198682/94‑1 (MQ=60)
                                                                                 CAACGCGGGTATCCCGTTCTCGGTGGTTCCGGGCATTACCGCAGCTTCTGGTTGCTCTGCCTATTCGGGTATTCCCCTCACGCATCGCGATTAT              <  SRR4302543.2654999/94‑1 (MQ=60)
                                                                                 CAACGCGGGTATCCCGTTCTCGGTGGTTCCGGGCATTACCGCAGCTTCTGGTTGCTCTGCCTATTCGGGTATTCCACTCACGCATCGCGATTAT              >  SRR4302543.3776573/1‑94 (MQ=60)
                                                                                 CAACGCGGGTATCCCGTTCTCGGTGGTTCCGGGCATTACCGCAGCTTCTGGTTGCTCTGCCTATTCGGGTATTCCACTCACGCATCGCGATTAT              <  SRR4302543.4466079/94‑1 (MQ=60)
                                                                                 CAACGCGGGTATCCCGTTCTCGGTGGTTCCGGGCATTACCGCAGCTTCTGGTTGCTCTGCCTATTCGGGTATTCCACTCACGCATCGCGATTAT              <  SRR4302543.639554/94‑1 (MQ=60)
                                                                                  AACGCTGGTATCCCGTGCTCGGTGGTTCCGGGCATTACCGCAGCTTCTGGTTGCTCTGCCTATTCGGGTATTCCACTCACGCATCGCGATTATG             <  SRR4302543.1980019/94‑1 (MQ=60)
                                                                                   ACGCGGGTATCCCGTTCTCGGTGGTTCCGGGCATTACCGCAGCTTCTGGTTGCTCTGCCTATTCGGGTATTCCACTCACGCATCGCGATTATGC            >  SRR4302543.4514247/1‑94 (MQ=60)
                                                                                    CGCGGGTATCCCGTTCTCGGTGGTTCCGGGCATTACCGCAGCTTCTGGTTGCTCTGCCTATTCGGGTATTCCACTCACGCATCGCGATTATGCC           <  SRR4302543.1254466/94‑1 (MQ=60)
                                                                                     GCGGGTATCCCGTTCTCGGTGGTTCCGGGCATTACCGCAGCTTCTGGTTGCTCTGCCTATTCGGGTATTCCACTCACGCATCGCGATTATGCCC          <  SRR4302543.3598796/94‑1 (MQ=60)
                                                                                      CGGGTATCTCGTTCTCGGTGGTTCCGGGCATTACCGCAGCTTCTGGTTGCTCTGCCTATTCGGGTATTCCACTCACGCATCGCGATTATGCCCA         <  SRR4302543.2450312/94‑1 (MQ=60)
                                                                                      CGGGTATCCCGTTCTCGGTGGTTCCGGGCATTACCGCAGCTTCTGGTTGCTCTGCCTATTCGGGTATTCCACTCACGCATCGCGATTATGCCCA         <  SRR4302543.4116211/94‑1 (MQ=60)
                                                                                      CGGGTATCCCGTTCTCGGTGGTTCCGGGCATTACCGCAGCTTCTGGTTGCTCTGCCTATTCGGGTATTCCACTCACGCATCGCGATTATGCCCA         >  SRR4302543.6361837/1‑94 (MQ=60)
                                                                                      CGGGTATCCCGTTCTCGGTGGTTCCGGGCATTACCGCAGCTTCTGGTTGCTCTGCCTATTCGGGTATTCCACTCACGCATCGCGATTATGCCCA         >  SRR4302543.65350/1‑94 (MQ=60)
                                                                                       GGGTATCCCGTTCTCGGTGGTTCCGGGCATTACCGCAGCTTCTGGTTGCTCTGCCTATTCGGGTATTCCACTCACGCATCGCGATTATGCCCAG        >  SRR4302543.7133798/1‑94 (MQ=60)
                                                                                       GGGTATCCCGTTCTCGGTGGTTCCGGGCATTACCGCAGCTTCTGGTTGCTCTGCCTATTCGGGTATTCCACTCACGCATCGCGATTATGCCCAG        <  SRR4302543.431933/94‑1 (MQ=60)
                                                                                        GGTATCCCGTTCTCGGTGGTTCCGGGCATTACCGCAGCTTCTGGTTGCTCTGCCTATTCGGGTATTCCACTCACGCATCGCGATTATGCCCAGA       >  SRR4302543.4592753/1‑94 (MQ=60)
                                                                                        GGTATCCCGTTCTCGGTGGTTCCGGGCATTACCGCAGCTTCTGGTTGCTCTGCCTATTCGGGTATTCCACTCACGCATCGCGATTATGCCCAGA       >  SRR4302543.5518667/1‑94 (MQ=60)
                                                                                         GTATCCCGTTCTCGGTGGTTCCGGGCATTACCGCAGCTTCTGGTTGCTCTGCCTATTCGGGTATTCCACTCACGCATCGCGATTATGCCCAGAG      <  SRR4302543.6568442/94‑1 (MQ=60)
                                                                                         GTATCCCGTTCTCGGTGGTTCCGGGCATTACCGCAGCTTCTGGTTGCTCTGCCTATTCGGGTACTCCACTCACGCATCGCGATTATGCCCAGAG      <  SRR4302543.6569922/94‑1 (MQ=60)
                                                                                          TATCCCGTTCTCGGTGGTTCCGGGCATTACCGCAGCTTCTGGTTGCTCTGCCTATTCGGGTATTCCACTCACGCATCGCGATTATGCCCAGAGC     <  SRR4302543.4743626/94‑1 (MQ=60)
                                                                                           ATCCCGTTCTCGGTGGTTCCGGGCATTACCGCAGCTTCTGGTTGCTCTGCCTATTCGGGTATTCCACTCACGCATCGCGATTATGCCCAGAGCG    <  SRR4302543.4238626/94‑1 (MQ=60)
                                                                                           ATCCCGTTCTCGGTGGTTCCGGGCATTACCGCAGCTTCTGGTTGCTCTGCCTATTCGGGTATTCCACTCACGCATCGCGATTATGCCCAGAGCG    <  SRR4302543.350962/94‑1 (MQ=60)
                                                                                             CCCGTTCTCGGTGGTTCCGGGCATTACCGCAGCTTCTGGTTGCTCTGCCTATTCGGGTATTCCACTCACGCATCGCGATTATGCCCAGAGCGTA  >  SRR4302543.4313418/1‑94 (MQ=60)
                                                                                             CCCGTTCTCGGTGGTTCCGGGCATTACCGCAGCTTCTGGTTGCTCTGCCTATTCGGGTATTCCACTCACGCATCGCGATTATGCCCAGAGCGTA  <  SRR4302543.6770610/94‑1 (MQ=60)
                                                                                             CCCGTTCTCGGTGGTTCCGGGCATTACCGCAGCTTCTGGTTGCTCTGCCTATTCGGGTATTCCACTCACGCATCGCGATTATGCCCAGAGCGTA  >  SRR4302543.3370178/1‑94 (MQ=60)
                                                                                             CCCGTTCTCGGTGGTTCCGGGCATTACCGCAGCTTCTGGTTGCTCTGCCTATTCGGGTATTCCACTCACGCATCGCGATTATGCCCAGAGCGTA  >  SRR4302543.4436980/1‑94 (MQ=60)
                                                                                             |                                                                                             
AAAAGGCAAACGCGTGGTGCGGCTGAAAGGTGGCGATCCGTTTATTTTTGGCCGTGGTGGCGAAGAGCTGGAAACACTGTGCAACGCGGGTATTCCGTTCTCGGTGGTTCCGGGTATTACCGCAGCTTCTGGTTGCTCTGCCTATTCGGGTATTCCACTCACGCATCGCGATTATGCCCAGAGCGTA  >  NC_000913/3496337‑3496523

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 9 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑