Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 1696 279.3 15874187 97.8% 15524954 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,596,295 A→G V332A (GTG→GCG)  b3460 ← LivJ

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,596,2950AG99.8% 1378.3 / ‑5.3 437V332A (GTG→GCG) b3460LivJ
Reads supporting (aligned to +/- strand):  ref base A (1/0);  new base G (185/250);  total (187/250)
Fisher's exact test for biased strand distribution p-value = 4.27e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.77e-01

Only 100 of 456 total aligned reads displayed.

GTGCCGTTGGCGTGCCAGTCAAATACGCCGAACTCAAAGCCTTTCAGATCGCCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCACGGAGTTCGCTTTCAGGTATTTGGCGATTTCAGCCGGATCGTCAGACTGATTCAGGCCCGCCTGCAAAGATTGCAGCGCGGCGTAGGTG  >  NC_000913/3596205‑3596384
                                                                                          |                                                                                         
gtgccgtTGGCGTGCCAGTCAAATACGCCGAACTCAAAGCCTTTCAGATCGCCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCcgcg                                                                                         <  2:2492297/93‑1 (MQ=255)
gtgccgtTGGCGTGCCAGTCAAATACGCCGAACTCAAAGCCTTTCAGATCGCCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCcgcg                                                                                         >  1:5650071/1‑93 (MQ=255)
gtgccgtTGGCGTGCCAGTCAAATACGCCGAACTCAAAGCCTTTCAGATCGCCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCcgcg                                                                                         >  1:4962489/1‑93 (MQ=255)
 tgccgtTGGCGTGCCAGTCAAATACGCCGAACTCAAAGCCTTTCAGATCGCCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCGCgg                                                                                        >  2:6439960/1‑93 (MQ=255)
 tgccgtTGGCGTGCCAGTCAAATACGCCGAACTCAAAGCCTTTCAGATCGCCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCGCgg                                                                                        >  1:1701277/1‑93 (MQ=255)
  gccgtTGGCGTGCCAGTCAAATACGCCGAACTCAAAGCCTTTCAGATCGCCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCGCGGa                                                                                       <  1:7776490/93‑1 (MQ=255)
     gtTGGCGTGCCAGTCAAATACGCCGAACTCAAAGCCTTTCAGATCGCCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCGCGGAGtt                                                                                    <  1:2497639/93‑1 (MQ=255)
     gtTGGCGTGCCAGTCAAATACGCCGAACTCAAAGCCTTTCAGATCGCCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCGCGGAGtt                                                                                    <  2:7518773/93‑1 (MQ=255)
      ttGGCGTGCCAGTCAAATACGCCGAACTCAAAGCCTTTCAGATCGCCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCGCGGAGTTc                                                                                   >  1:2720715/1‑93 (MQ=255)
          cGTGCCAGTCAAATACGCCGAACTCAAAGCCTTTCAGATCGCCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCGCGGAGTTCGCtt                                                                               <  2:2840704/93‑1 (MQ=255)
           gTGCCAGTCAAATACGCCGAACTGAAAGCCTTTCAGATCGCCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCGCGGAGTTCGCttt                                                                              <  2:5639191/93‑1 (MQ=255)
           gTGCCAGTCAAATACGCCGAACTCAAAGCCTTTCAGATCGCCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCGCGGAGTTCGCttt                                                                              >  1:1801458/1‑93 (MQ=255)
           gTGCCAGTCAAATACGCCGAACTCAAAGCCTTTCAGATCGCCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCGCGGAGTTCGCttt                                                                              >  1:3996151/1‑93 (MQ=255)
            tGCCAGTCAAATACGCCGAACTCAAAGCCTTTCAGATCGCCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCGCGGAGTTCGCTTTc                                                                             <  1:4784493/93‑1 (MQ=255)
            tGCCAGTCAAATACGCCGAACTCAAAGCCTTTCAGATCGCCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCGCGGAGTTCGCTTTc                                                                             <  1:4084247/93‑1 (MQ=255)
            tGCCAGTCAAATACGCCGAACTCAAAGCCTTTCAGATCGCCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCGCGGAGTTCGCTTTc                                                                             >  1:3065465/1‑93 (MQ=255)
             gCCAGTCAAATACGCCGAACTCAAAGCCTTTCAGATCGCCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCGCGGAGTTCGCTTTCa                                                                            <  2:2348492/93‑1 (MQ=255)
                aGTCAAATACGCCGAACTCAAAGCCTTTCAGATCGCCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCGCGGAGTTCGCTTTCAGGt                                                                         >  1:1011369/1‑93 (MQ=255)
                    aaaTACGCCGAACTCAAAGCCTTTCAGATCGCCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCGCGGAGTTCGCTTTCAGGTAttt                                                                     <  2:310826/93‑1 (MQ=255)
                    aaaTACGCCGAACTCAAAGCCTTTCAGATCGCCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCGCGGAGTTCGCTTTCAGGTAttt                                                                     <  2:6152261/93‑1 (MQ=255)
                     aaTACGCCGAACTCAAAGCCTTTCAGATCGCCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCGCGGAGTTCGCTTTCAGGTATTTg                                                                    >  2:635322/1‑93 (MQ=255)
                      aTACGCCGAACTCAAAGCCTTTCAGATCGCCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCGCGGAGTTCGCTTTCAGGTATTTgg                                                                   >  2:6235569/1‑93 (MQ=255)
                       tACGCCGAACTCAAAGCCTTTCAGATCGCCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCGCGGAGTTCGCTTTCAGGTATTTGGc                                                                  <  1:590869/93‑1 (MQ=255)
                       tACGCCGAACTCAAAGCCTTTCAGATCGCCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCGCGGAGTTCGCTTTCAGGTATTTGGc                                                                  >  2:6783545/1‑93 (MQ=255)
                       tACGCCGAACTCAAAGCCTTTCAGATCGCCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCGCGGAGTTCGCTTTCAGGTATTTGGc                                                                  <  2:1662902/93‑1 (MQ=255)
                         cGCCGAACTCAAAGCCTTTCAGATCGCCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCGCGGAGTTCGCTTTCAGGTATTTGGCGa                                                                <  2:7276606/93‑1 (MQ=255)
                          gCCGAACTCAAAGCCTTTCAGATCGCCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCGCGGAGTTCGCTTTCAGGTATTTGGCGAt                                                               >  2:3161058/1‑93 (MQ=255)
                          gCCGAACTCAAAGCCTTTCAGATCGCCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCGCGGAGTTCGCTTTCAGGTATTTGGCGAt                                                               <  1:5319891/93‑1 (MQ=255)
                            cGAACTCAAAGCCTTTCAGATCGCCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCGCGGAGTTCGCTTTCAGGTATTTGGCGAttt                                                             >  1:4230296/1‑93 (MQ=255)
                             gAACTCAAAGCCTTTCAGATCGCCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCGCGGAGTTCGCTTTCAGGTATTTGGCGATTTc                                                            >  2:5559704/1‑93 (MQ=255)
                              aaCTCAAAGCCTTTCAGATCGCCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCGCGGAGTTCGCTTTCAGGTATTTGGCGATTTCa                                                           <  1:3323746/93‑1 (MQ=255)
                               aCTCAAAGCCTTTCAGATCGCCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCGCGGAGTTCGCTTTCAGGTATTTGGCGATTTCAg                                                          >  2:6629172/1‑93 (MQ=255)
                               aCTCAAAGCCTTTCAGATCGCCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCGCGGAGTTCGCTTTCAGGTATTTGGCGATTTCAg                                                          >  1:6379299/1‑93 (MQ=255)
                                cTCAAAGCCTTTCAGATCGCCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCGCGGAGTTCGCTTTCAGGTATTTGGCGATTTCAGc                                                         >  1:2811682/1‑93 (MQ=255)
                                cTCAAAGCCTTTCAGATCGCCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCGCGGAGTTCGCTTTCAGGTATTTGGCGATTTCAGc                                                         <  1:3786312/93‑1 (MQ=255)
                                  cAAAGCCTTTCAGATCGCCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCGCGGAGTTCGCTTTCAGGTATTTGGCGATTTCAGCCg                                                       >  2:6062817/1‑93 (MQ=255)
                                   aaaGCCTTTCAGATCGCCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCGCGGAGTTCGCTTTCAGGTATTTGGCGATTTCAGCCgg                                                      <  1:1450578/93‑1 (MQ=255)
                                    aaGCCTTTCAGATCGCCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCGCGGAGTTCGCTTTCAGGTATTTGGCGATTTCAGCCGGa                                                     >  2:5197419/1‑93 (MQ=255)
                                    aaGCCTTTCAGATCGCCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCGCGGAGTTCGCTTTCAGGTATTTGGCGATTTCAGCCGGa                                                     >  2:5060873/1‑93 (MQ=255)
                                    aaGCCTTTCAGATCGCCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCGCGGAGTTCGCTTTCAGGTATTTGGCGATTTCAGCCGGa                                                     <  2:6997941/93‑1 (MQ=255)
                                     aGCCTTTCAGATCGCCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCGCGGAGTTCGCTTTCAGGTATTTGGCGATTTCAGCCGGAt                                                    <  1:7506753/93‑1 (MQ=255)
                                       ccTTTCAGATCGCCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCGCGGAGTTCGCTTTCAGGTATTTGGCGATTTCAGCCGGATCg                                                  <  1:232429/93‑1 (MQ=255)
                                       ccTTTCAGATCGCCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCGCGGAGTTCGCTTTCAGGTATTTGGCGATTTCAGCCGGATCg                                                  <  2:3819866/93‑1 (MQ=255)
                                         tttCAGATCGCCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCGCGGAGTTCGCTTTCAGGTATTTGGCGATTTCAGCCGGATCGTc                                                <  1:6719925/93‑1 (MQ=255)
                                         tttCAGATCGCCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCGCGGAGTTCGCTTTCAGGTATTTGGCGATTTCAGCCGGATCGTc                                                >  2:2053954/1‑93 (MQ=255)
                                         tttCAGATCGCCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCGCGGAGTTCGCTTTCAGGTATTTGGCGATTTCAGCCGGATCGTc                                                >  2:4260076/1‑93 (MQ=255)
                                         tttCAGATCGCCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCGCGGAGTTCGCTTTCAGGTATTTGGCGATTTCAGCCGGATCGTc                                                >  2:7883967/1‑93 (MQ=255)
                                          ttCAGATCGCCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCGCGGAGTTCGCTTTCAGGTATTTGGCGATTTCAGCCGGATCGTCa                                               <  2:2631481/93‑1 (MQ=255)
                                           tCAGATCGCCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCGCGGAGTTCGCTTTCAGGTATTTGGCGATTTCAGCCGGATCGTCAg                                              >  1:2047904/1‑93 (MQ=255)
                                            cAGATCGCCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCGCGGAGTTCGCTTTCAGGTATTTGGCGATTTCAGCCGGATCGTCAGa                                             >  1:606571/1‑93 (MQ=255)
                                              gATCGCCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCGCGGAGTTCGCTTTCAGGTATTTGGCGATTTCAGCCGGATCGTCAGACt                                           >  1:1753472/1‑93 (MQ=255)
                                               aTCGCCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCGCGGAGTTCGCTTTCAGGTATTTGGCGATTTCAGCCGGATCGTCAGACTg                                          <  1:114151/93‑1 (MQ=255)
                                                tCGCCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCGCGGAGTTCGCTTTCAGGTATTTGGCGATTTCAGCCGGATCGTCAGACTGa                                         <  2:3979908/93‑1 (MQ=255)
                                                tCGCCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCGCGGAGTTCGCTTTCAGGTATTTGGCGATTTCAGCCGGATCGTCAGACTGa                                         <  1:1205958/93‑1 (MQ=255)
                                                tCGCCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGGATCCGCGGAGTTCGCTTTCAGGTATTTGGCGATTTCAGCCGGATCGTCAGACTGa                                         >  1:7406613/1‑93 (MQ=255)
                                                 cGCCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCTCGGAGTTCGCTTTCAGGTATTTGGCGATTTCAGCCGGATCGTCAGACTGAt                                        >  1:3747059/1‑93 (MQ=255)
                                                  gCCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCGCGGAGTTCGCTTTCAGGTATTTGGCGATTTCAGCCGGATCGTCAGACTGAtt                                       >  1:3466685/1‑93 (MQ=255)
                                                  gCCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCGCGGAGTTCGCTTTCAGGTATTTGGCGATTTCAGCCGGATCGTCAGACTGAtt                                       <  1:415481/93‑1 (MQ=255)
                                                  gCCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCGCGGAGTTCGCTTTCAGGTATTTGGCGATTTCAGCCGGATCGTCAGACTGAtt                                       <  1:5071064/93‑1 (MQ=255)
                                                  gCCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCGCGGAGTTCGCTTTCAGGTATTTGGCGATTTCAGCCGGATCGTCAGACTGAtt                                       <  2:1031143/93‑1 (MQ=255)
                                                   ccTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCGCGGAGTTCGCTTTCAGGTATTTGGCGATTTCAGCCGGATCGTCAGACTGATTc                                      <  1:3577155/93‑1 (MQ=255)
                                                   ccTTTCTCATCCCAGGTCAGCGGCCCCATTACGGTATCCGCGGAGTTCGCTTTCAGGTATTTGGCGATTTCAGCCGGATCGTCAGACTGATTc                                      <  2:1265496/93‑1 (MQ=255)
                                                    cTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCGCGGAGTTCGCTTTCAGGTATTTGGCGATTTCAGCCGGATCGTCAGACTGATTCa                                     <  1:2928904/93‑1 (MQ=255)
                                                    cTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCGCGGAGTTCGCTTTCAGGTATTTGGCGATTTCAGCCGGATCGTCAGACTGATTCa                                     <  1:5567753/93‑1 (MQ=255)
                                                    cTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCGCGGAGTTCGCTTTCAGGTATTTGGCGATTTCAGCCGGATCGTCAGACTGATTCa                                     <  1:368167/93‑1 (MQ=255)
                                                    cTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCGCGGAATTCGCTTTCAGGTATTTGGCGATTTCAGCCGGATCGTCAGACTGATTCa                                     <  1:1027563/93‑1 (MQ=255)
                                                     tttCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCGCGGAGTTCGCTTTCAGGTATTTGGCGATTTCAGCCGGATCGTCAGACTGATTCAg                                    <  1:163554/93‑1 (MQ=255)
                                                     tttCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCGCGGAGTTCGCTTTCAGGTATTTGGCGATTTCAGCCGGATCGTCAGACTGATTCAg                                    >  1:1899370/1‑93 (MQ=255)
                                                     tttCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCGCGGAGTTCGCTTTCAGGTATTTGGCGATTTCAGCCGGATCGTCAGACTGATTCAg                                    >  1:2401838/1‑93 (MQ=255)
                                                       tctcATCCCAGGTCAGCGGTCCCATTACGGTATCCGCGGAGTTCGCTTTCAGGTATTTGGCGATTTCAGCCGGATCGTCAGACTGATTCAGGc                                  <  1:69618/93‑1 (MQ=255)
                                                       tctcATCCCAGGTCAGCGGTCCCATTACGGTATCCGCGGAGTTCGCTTTCAGGTATTTGGCGATTTCAGCCGGATCGTCAGACTGATTCAGGc                                  >  2:3752388/1‑93 (MQ=255)
                                                        ctcATCCCAGGTCAGCGGTCCCATTACGGTATCCGCGGAGTTCGCTTTCAGGTATTTGGCGATTTCAGCCGGATCGTCAGACTGATTCAGGcc                                 <  2:7460567/93‑1 (MQ=255)
                                                            tCCCAGGTCAGCGGTCCCATTACGGTATCCGCGGAGTTCGCTTTCAGGTATTTGGCGATTTCAGCCGGATCGTCAGACTGATTCAGGCCCGcc                             <  2:4087343/93‑1 (MQ=255)
                                                            tCCCAGGTCAGCGGTCCCATTACGGTATCCGCGGAGTTCGCTTTCAGGTATTTGGCGATTTCAGCCGGATCGTCAGACTGATTCAGGCCCGcc                             >  2:4426797/1‑93 (MQ=255)
                                                               cAGGTCAGCGGTCCCATTACGGTATCCGCGGAGTTCGCTTTCAGGTATTTGGCGATTTCAGCCGGATCGTCAGACTGATTCAGGCCCGCCTGc                          >  2:3514853/1‑93 (MQ=255)
                                                               cAGGTCAGCGGTCCCATTACGGTATCCGCGGAGTTCGCTTTCAGGTATTTGGCGATTTCAGCCGGATCGTCAGACTGATTCAGGCCCGCCTGc                          >  1:383617/1‑93 (MQ=255)
                                                                 ggTCAGCGGTCCCATTACGGTATCCGCGGAGTTCGCTTTCAGGTATTTGGCGATTTCAGCCGGATCGTCAGACTGATTCAGGCCCGCCTGCaa                        >  1:6563858/1‑93 (MQ=255)
                                                                 cgTCAGCGGTCACATTACGGTATCCGCGGAGTTCGCTTTCAGGTATTTGGGGATTTCAGCCGGATCGTCAGACTGATTCAGGCCCGCCTGCaa                        <  2:5933206/92‑1 (MQ=255)
                                                                  gTCAGCGGTCCCATTACGGTATCCGCGGAGTTCGCTTTCAGGTATTTGGCGATTTCAGCCGGATCGTCAGACTGATTCAGGCCCGCCTGCaaa                       >  2:5146019/1‑93 (MQ=255)
                                                                  gTCAGCGGTCCCATTACGGTATCCGCGGAGTTCGCTTTCAGGTATTTGGCGATTTCAGCCGGATCGTCAGACTGATTCAGGCCCGCCTGCaaa                       >  1:7914643/1‑93 (MQ=255)
                                                                    cAGCGGTCCCATTACGGTATCCGCGGAGTTCGCTTTCAGGTATTTGGCGATTTCAGCCGGATCGTCAGACTGATTCAGGCCCGCCTGCAAAGa                     <  1:724824/93‑1 (MQ=255)
                                                                    cAGCGGTCCCATTACGGTATCCGCGGAGTTCGCTTTCAGGTATTTGGCGATTTCAGCCGGATCGTCAGACTGATTCAGGCCCGCCTGCAAAGa                     <  1:927068/93‑1 (MQ=255)
                                                                      gCGGTCCCATTACGGTATCCGCGGAGTTCGCTTTCAGGTATTTGGCGATTTCAGCCGGATCGTCAGACTGATTCAGGCCCGCCTGCAAAGAtt                   <  2:4758846/93‑1 (MQ=255)
                                                                      gCGGTCCCATTACGGGATCCGCGGAGTTCGCTTTCAGGTATTTGGCGATTTCAGCCGGATCGTCAGACTGATTCAGGCACGCCCGCAAAGAtt                   >  2:578869/1‑93 (MQ=255)
                                                                        ggTCCCATTACGGTATCCGCGGAGTTCGCTTTCAGGTAGTTGGCGATTTCAGCCGGATCGTCAGACTGATTCAGGCCCGCCTGCAAAGATTGc                 <  2:4618925/93‑1 (MQ=255)
                                                                         gTCCCATTACGGTATCCGCGGAGTTCGCTTTCAGGTATTTGGCGATTTCAGCCGGATCGTCAGACTGATTCAGGCCCGCCTGCAAAGATTGCa                <  2:876699/93‑1 (MQ=255)
                                                                         gTCCCATTACGGTATCCGCGGAGTTCGCTTTCAGGTATTTGGCGATTTCAGCCGGATCGTCAGACTGATTCAGGCCCGCCTGCAAAGATTGCa                >  2:1542289/1‑93 (MQ=255)
                                                                         gTCCCATTACGGTATCCGCGGAGTTCGCTTTCAGGTATTTGGCGATTTCAGCCGGATCGTCAGACTGATTCAGGCCCGCCTGCAAAGATTGCa                >  2:5249109/1‑93 (MQ=255)
                                                                           ctcATTACGGTATCCGCGGAGTTCGCTTGCAGGTATTTGGCGATTTCAGCCGGATCGTCAGACTGATTCAGGCCCGCCTGCAAAGATTGCAgc              <  2:3350676/91‑1 (MQ=255)
                                                                            ccATTACGGTATCCGCGGAGTTCGCTTTCAGGTATTTGGCGATTTCAGCCGGATCGTCAGACTGATTCAGGCCCGCCTGCAAAGATTGCAgcg             >  1:4604364/1‑93 (MQ=255)
                                                                             cATTACGGTATCCGCGGAGTTCGCTTTCAGGTATTTGGCGATTTCAGCCGGATCGTCAGACTGATTCAGGCCCGCCTGCAAAGATTGCAgcgc            <  2:1973771/93‑1 (MQ=255)
                                                                             cATTACGGTATCCGCGGAGTTCGCTTTCAGGTATTTGGCGATTTCAGCCGGATCGTCAGACTGATTCAGGCCCGCCTGCAAAGATTGCAgcgc            <  2:2270204/93‑1 (MQ=255)
                                                                              aTTACGGTATCCGCGGAGTTCGCTTTCAGGTATTTGGCGATTTCAGCCGGATCGTCAGACTGATTCACTGCCGCCTGCAAAGATTGCAGcgcg           >  1:4461830/1‑93 (MQ=255)
                                                                               ttACGGTATCCACGGAGTTCGCTTTCAGGTATTTGGCGATTTCAGCCGGATCGTCAGACTGATTCAGGCCCGCCTGCAAAGATTGCAGCgcgg          >  2:5430097/1‑93 (MQ=255)
                                                                                  cGGTATCCGCGGAGTTCGCTTTCAGGTATTTGGCGATTTCAGCCGGATCGTCAGACTGATTCAGGCCCGCCTGCAAAGATTGCAGCGCGGCGt       <  2:2901626/93‑1 (MQ=255)
                                                                                   ggTATCCGCGGAGTTCGCTTTCAGGTATTTGGCGATTTCAGCCGGATCGTCAGACTGATTCAGGCCCGCCTGCAAAGATTGCAGCGCGGCGTa      >  2:2147706/1‑93 (MQ=255)
                                                                                    gTATCCGCGGAGTTCGCTTTCAGGTATTTGGCGATTTCAGCCGGATCGTCAGACTGATTCAGGCCCGCCTGCAAAGATTGCAGCGCGGCGTAg     <  2:3723965/93‑1 (MQ=255)
                                                                                       tCCGCGGAGTTCGCTTTCAGGTATTTGGCGATTTCAGCCGGATCGTCAGACTGATTCAGGCCCGCCTGCAAAGATTGCAGCGCGGCGTAggtg  <  2:6564207/93‑1 (MQ=255)
                                                                                       tCCGCGGAGTTCGCTTTCAGGTATTTGGCGATTTCAGCCGGATCGTCAGACTGATTCAGGCCCGCCTGCAAAGATTGCAGCGCGGCGTAggtg  <  2:6705351/93‑1 (MQ=255)
                                                                                       tCCGCGGAGTTCGCTTTCAGGTATTTGGCGATTTCAGCCGGATCGTCAGACTGATTCAGGCCCGCCTGCAAAGATTGCAGCGCGGCGTAggtg  >  1:4334023/1‑93 (MQ=255)
                                                                                          |                                                                                         
GTGCCGTTGGCGTGCCAGTCAAATACGCCGAACTCAAAGCCTTTCAGATCGCCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCACGGAGTTCGCTTTCAGGTATTTGGCGATTTCAGCCGGATCGTCAGACTGATTCAGGCCCGCCTGCAAAGATTGCAGCGCGGCGTAGGTG  >  NC_000913/3596205‑3596384

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output Only 200 of 237 total aligned reads displayed.
GCCGTGCCGTTGGCGTGCCAGTCAAATACGCCGAACTCAAAGCCTTTCAGATCGCCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCACGGAGTTCGCTTTCAGGTATTTGGCGATTTCAGCCGGATCGTCAGACTGATTCAGGCCCGCCTGCAAAGATTGCAGCGCGGCGTAGGTGGTCC  >  NC_000913/3596202‑3596388
                                                                                             |                                                                                             
GCCGTGCCGTTGGCGTGCCAGTCAAATACGCCGAACTCAAAGCCTTTCAGATCGCCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCG                                                                                               >  SRR4302543.2616019/1‑94 (MQ=60)
GCCGTGCCGTTGGCGTGCCAGTCAAATACGCCGAACTCAAAGCCTTTCAGATCGCCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCG                                                                                               <  SRR4302543.1149550/94‑1 (MQ=60)
 CCGTGCCGTTGGCGTGCCAGTCAAATACGCCGAACTCAAAGCCTTTCAGATCGCCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCGC                                                                                              <  SRR4302543.3853394/94‑1 (MQ=60)
  CGTGCCGTTGGCGTGCCAGTCAAATACGCCGAACTCAAAGCCTTTCAGATCGCCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCGCG                                                                                             >  SRR4302543.5701578/1‑94 (MQ=60)
  CGTGCCGTTGGCGTGCCAGTCAAATACGCCGAACTCAAAGCCTTTCAGATCGCCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCGCG                                                                                             <  SRR4302543.7202024/94‑1 (MQ=60)
  CGTGCCGTTGGCGTGCCAGTCAAATACGCCGAACTCAAAGCCTTTCAGATCGCCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCGCG                                                                                             >  SRR4302543.5007664/1‑94 (MQ=60)
  CGTGCCGTTGGCGTGCCAGTCAAATACGCCGAACTCAAAGCCTTTCAGATCGCCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCGCG                                                                                             >  SRR4302543.889571/1‑94 (MQ=60)
  CGTGCCGTTGGCGTGCCAGTCAAATACGCCGAACTCAAAGCCTTTCAGATCGCCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCGCG                                                                                             <  SRR4302543.4065075/94‑1 (MQ=60)
   GTGCCGTTGGCGTGCCAGTCAAATACGCCGAACTCAAAGCCTTTCAGATCGCCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCGCGG                                                                                            >  SRR4302543.1716520/1‑94 (MQ=60)
     TCCGTTGGCGTGCCAGTCAAATACGCCGAACTCAAAGCCTTTCAGATCGCCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCGCGGAG                                                                                          <  SRR4302543.1935821/94‑1 (MQ=60)
     GCCGTTGGCGTGCCAGTCAAATACGCCGAACTCAAAGCCTTTCAGATCGCCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCGCGGAG                                                                                          <  SRR4302543.7720285/94‑1 (MQ=60)
     GCCGTTGGCGTGCCAGTCAAATACGCCGAACTCAAAGCCTTTCAGATCGCCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCGCGGAG                                                                                          <  SRR4302543.4212007/94‑1 (MQ=60)
     GCCGTTGGCGTGCCAGTCAAATACGCCGAACTCAAAGCCTTTCAGATCGCCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCGCGGAG                                                                                          <  SRR4302543.7849832/94‑1 (MQ=60)
     GCCGTTGGCGTGCCAGTCAAATACGCCGAACTCAAAGCCTTTCAGATCGCCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCGCGGAG                                                                                          <  SRR4302543.1610242/94‑1 (MQ=60)
     GCCGTTGGCGTGCCAGTCAAATACGCCGAACTCAAAGCCTTTCAGATCGCCTTTCACATCCCAGGTCAGCGGTCCCATTACGGTATCCGCGGAG                                                                                          <  SRR4302543.7791880/94‑1 (MQ=60)
      CCGTTGGCGTGCCAGTCAAATACGCCGAACTCAAAGCCTTTCAGATCGCCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCGCGGAGT                                                                                         >  SRR4302543.729400/1‑94 (MQ=60)
      CCGTTGGCGTGCCAGTCAAATACGCCGAACTCAAAGCCTTTCAGATCGCCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCGCGGAGT                                                                                         >  SRR4302543.5084575/1‑94 (MQ=60)
       CGTTGGCGTGCCAGTCAAATACGCCGAACTCAAAGCCTTTCAGATCGCCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCGCGGAGTT                                                                                        <  SRR4302543.4746455/94‑1 (MQ=60)
       CGTTGGCGTGCCAGTCAAATACGCCGAACTCAAAGCCTTTCAGATCGCCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCGCGGAGTT                                                                                        <  SRR4302543.3826870/94‑1 (MQ=60)
       CGTTGGCGTGCCAGTCAAATACGCCGAACTCAAAGCCTTTCAGATCGCCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCGCGGAGTT                                                                                        <  SRR4302543.3827074/94‑1 (MQ=60)
        GTTGGCGTGCCAGTCAAATACGCCGAACTCAAAGCCTTTCAGATCGCCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCGCGGAGTTC                                                                                       >  SRR4302543.2307464/1‑94 (MQ=60)
        GTTGGCGTGCCAGTCAAATACGCCGAACTCAAAGCCTTTCAGATCGCCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCGCGGAGTTC                                                                                       >  SRR4302543.2745454/1‑94 (MQ=60)
           GGCGTGCCAGTCAAATACGCCGAACTCAAAGCCTTTCAGATCGCCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCGCGGAGTTCGCT                                                                                    <  SRR4302543.169584/94‑1 (MQ=60)
           GGCGTGCCAGTCAAATACGCCGAACTCAAAGCCTTTCAGATCGCCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCGCGGAGGTCGCT                                                                                    >  SRR4302543.1770511/1‑94 (MQ=60)
            GCGTGCCAGTCAAATACGCCGAACTCAAAGCCTTTCAGATCGCCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCGCGGAGTTCGCTT                                                                                   <  SRR4302543.3560783/94‑1 (MQ=60)
            GCGTGCCAGTCAAATACGCCGAACTCAAAGCCTTTCAGATCGCCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCGCGGAGTTCGCTT                                                                                   <  SRR4302543.1147940/94‑1 (MQ=60)
            GCGTGCCAGTCAAATACGCCGAACTCAAAGCCTTTCAGATCGCCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCGCGGAGTTCGCTT                                                                                   <  SRR4302543.3062688/94‑1 (MQ=60)
             CGTGCCAGTCAAATACGCCGAACTCAAAGCCTTTCAGATCGCCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCGCGGAGTTCGCTTT                                                                                  >  SRR4302543.4031761/1‑94 (MQ=60)
             CGTGCCAGTCAAATACGCCGAACTCAAAGCCTTTCAGATCGCCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCGCGGAGTTCGCTTT                                                                                  >  SRR4302543.1817586/1‑94 (MQ=60)
              GTGCCAGTCAAATACGCCGAACTCAAAGCCTTTCAGATCGCCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCGCGGAGTTCGCTTTC                                                                                 <  SRR4302543.6048680/94‑1 (MQ=60)
              GTGCCAGTCAAATACGCCGAACTCAAAGCCTTTCAGATCGCCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCGCGGAGTTCGCTTTC                                                                                 >  SRR4302543.6623388/1‑94 (MQ=60)
               TGCCAGTCAAATACGCCGAACTCAAAGCCTTTCAGATCGCCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCGCGGAGTTCGCTTTCA                                                                                <  SRR4302543.4828226/94‑1 (MQ=60)
               TGCCAGTCAAATACGCCGAACTCAAAGCCTTTCAGATCGCCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCGCGGAGTTCGCTTTCA                                                                                <  SRR4302543.4120863/94‑1 (MQ=60)
                GCCAGTCAAATACGCCGAACTCAAAGCCTTTCAGATCGCCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCGCGGAGTTCGCTTTCAG                                                                               <  SRR4302543.1547430/94‑1 (MQ=60)
                  CAGTCAAATACGCCGAACTCAAAGCCTTTCAGATCGCCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCGCGGAGTTCGCTTTCAGGT                                                                             >  SRR4302543.1020317/1‑94 (MQ=60)
                  CAGTCAAATACGCCGAACTCAAAGCCTTTCAGATCGCCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCGCGGAGTTCGCTTTCAGGT                                                                             >  SRR4302543.5774547/1‑94 (MQ=60)
                  CAGTCAAATACGCCGAACTCAAAGCCTTTCAGATCGCCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCGCGGAGTTCGCTTTCAGGT                                                                             >  SRR4302543.5596248/1‑94 (MQ=60)
                  CAGTCAAATACGCCGAACTCAAAGCCTTTCAGATCGCCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCGCGGAGTTCGCTTTCAGGT                                                                             >  SRR4302543.6574/1‑94 (MQ=60)
                    GTCAAATACGCCGAACTCAAAGCCTTTCAGATCGCCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCGCGGAGTTCGCTTTCAGGTAT                                                                           <  SRR4302543.2704544/94‑1 (MQ=60)
                      CAAATACGCCGAACTCAAAGCCTTTCAGATCGCCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCGCTGAGTTCGCTTTCAGGTATTT                                                                         >  SRR4302543.7928738/1‑94 (MQ=60)
                      CAAATACGCCGAACTCAAAGCCTTTCAGATCGCCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCGCGGAGTTCGCTTTCAGGTATTT                                                                         >  SRR4302543.890205/1‑94 (MQ=60)
                      CAAATACGCCGAACTCAAAGCCTTTCAGATCGCCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCGCGGAGTTCGCTTTCAGGTATTT                                                                         <  SRR4302543.1678742/94‑1 (MQ=60)
                      CAAATACGCCGAACGCAAAGCCTTTCAGATCGCCTTTCTCACCCCAGGGCAGCGGGCCCATTACGGTATCCGCGGAGTTCGCTTTCAGGTATTT                                                                         <  SRR4302543.7703690/94‑1 (MQ=60)
                       AAATACGCCGAACTCAAAGCCTTTCAGATCGCCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCGCGGAGTTCGCTTTCAGGTATTTG                                                                        <  SRR4302543.976636/94‑1 (MQ=60)
                       AAATACGCCGAACTCAAAGCCTTTCAGATCGCCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCGCGGAGTTCGCTTTCAGGTATTTG                                                                        >  SRR4302543.2797055/1‑94 (MQ=60)
                       AAATACGCCGAACTCAAAGCCTTTCAGATCGCCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCGCGGAGTTCGCTTTCAGGTATTTG                                                                        <  SRR4302543.7427137/94‑1 (MQ=60)
                       AAATACGCCGAACTCAAAGCCTTTCAGATCGCCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCGCGGAGTTCGCTTTCAGGTATTTG                                                                        >  SRR4302543.1875106/1‑94 (MQ=60)
                       AAATACGCCGAACTCAAAGCCTTTCAGATCGCCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCGCGGAGTTCGCTTTCAGGTATTTG                                                                        >  SRR4302543.1992610/1‑94 (MQ=60)
                       AAATACGCCGAACTCAAAGCCTTTCAGATCGCCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCGCGGAGTTCGCTTTCAGGTATTTG                                                                        >  SRR4302543.2111159/1‑94 (MQ=60)
                         ATACGCCGAACTCAAAGCCTTTCAGATCGCCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCGCCGAGTTCGCTTTCAGGTATTTGGC                                                                      >  SRR4302543.4204432/1‑94 (MQ=60)
                          TACGCCGAACTCAAAGCCTTTCAGATCGCCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCGCGGAGTTCGCTTTCAGGTATTTGGCG                                                                     <  SRR4302543.596297/94‑1 (MQ=60)
                          TACGCCGAACTCAAAGCCTTTCAGATCGCCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCGCGGAGTTCGCTTTCAGGTATTTGGCG                                                                     <  SRR4302543.2213021/94‑1 (MQ=60)
                            CGCCGAACTCAAAGCCTTTCAGATCGCCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCGCGGAGTTCGCTTTCAGGTATTTGGCGAT                                                                   >  SRR4302543.1519306/1‑94 (MQ=60)
                             GCCGAACTCAAAGCCTTTCAGATCGCCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCGCGGAGTTCGCTTTCAGGTATTTGGCGATT                                                                  <  SRR4302543.5367958/94‑1 (MQ=60)
                              CCGAACTCAAAGCCTTTCAGATCGCCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCGCGGAGTTCGCTTTCAGGTATTTGGCGATTT                                                                 <  SRR4302543.1038860/94‑1 (MQ=60)
                              CCGAACTCAAAGCCTTTCAGATCGCCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCGCGGAGTTCGCTTTCAGGTATTTGGCGATTT                                                                 >  SRR4302543.4268670/1‑94 (MQ=60)
                               CGAACTCAAAGCCTTTCAGATCGCCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCGCGGAGTTCGCTTTCAGGTATTTGGCGATTTC                                                                >  SRR4302543.6330559/1‑94 (MQ=60)
                               CGAACTCAAAGCCTTTCAGATCGCCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCGCGGAGTTCGCTTTCAGGTATTTGGCGATTTC                                                                >  SRR4302543.1361124/1‑94 (MQ=60)
                                GAACTCAAAGCCTTTCAGATCGCCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCGCGGAGTTCGCTTTCAGGTATTTGGCGATTTCA                                                               <  SRR4302543.440617/94‑1 (MQ=60)
                                GAACTCAAAGCCTTTCAGATCGCCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCGCGGAGTTCGCTTTCAGGTATTTGGCGATTTCA                                                               <  SRR4302543.4608397/94‑1 (MQ=60)
                                GAACTCAAAGCCTTTCAGATCGCCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCGCGGAGTTCGCTTTCAGGTATTTGGCGATTTCA                                                               <  SRR4302543.4303071/94‑1 (MQ=60)
                                CAACTCAAAGCCTTTCAGATCGCCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCGCGGAGTTCGCTTTCAGGTATTTGGCGATTTCA                                                               <  SRR4302543.2640658/94‑1 (MQ=60)
                                 AACTCAAAGCCTTTCAGATCGCCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCGCGGAGTTCGCTTTCAGGTATTTGGCGATTTCAG                                                              <  SRR4302543.3060767/94‑1 (MQ=60)
                                 AACTCAAAGCCTTTCAGATCGCCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCGCGGAGTTCGCTTTCAGGTATTTGGCGATTTCAG                                                              >  SRR4302543.6439324/1‑94 (MQ=60)
                                 AACTCAAAGCCTTTCAGATCGCCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCGCGGAGTTCGCTTTCAGGTATTTGGCGATTTCAG                                                              <  SRR4302543.3353729/94‑1 (MQ=60)
                                 AACTCAAAGCCTTTCAGATCGCCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCGCGGAGTTCGCTTTCAGGTATTTGGCGATTTCAG                                                              <  SRR4302543.3449141/94‑1 (MQ=60)
                                  ACTCAAAGCCTTTCAGATCGCCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCGCGGAGTTCGCTTTCAGGTATTTGGCGATTTCAGC                                                             >  SRR4302543.2837427/1‑94 (MQ=60)
                                   CTCAAAGCCTTTCAGATCGCCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCGCGGAGTTCGCTTTCAGGTATTTGGCGATTTCAGCC                                                            <  SRR4302543.3819666/94‑1 (MQ=60)
                                   CTCAAAGCCTTTCAGATCGCCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCGCGGAGTTCGCTTTCAGGTATTTGGCGATTTCAGCC                                                            >  SRR4302543.169610/1‑94 (MQ=60)
                                   CTCAAAGCCTTTCAGATCGCCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCGCGGAGTTCGCTTTCAGGTATTTGGCGATTTCAGCC                                                            <  SRR4302543.1784311/94‑1 (MQ=60)
                                   CTCAAAGCCTTTCAGATCGCCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCGCGGAGTTCGCTTTCAGGTATTTGGCGATTTCAGCC                                                            <  SRR4302543.2357361/94‑1 (MQ=60)
                                   CTCAAAGCCTTTCAGATCGCCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCGCGGAGTTCGCTTTCAGGTATTTGGCGATTTCAGCC                                                            >  SRR4302543.5314569/1‑94 (MQ=60)
                                   CTCAAAGCCTTTCAGATCGCCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGGATCCGCGGAGTTCGCTTTCAGGTATTTGGCGATTTCAGCC                                                            >  SRR4302543.4303974/1‑94 (MQ=60)
                                    TCAAAGCCTTTCAGATCGCCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCGCGGAGTTCGCTTTCAGGTATTTGGCGATTTCAGCCG                                                           <  SRR4302543.1262859/94‑1 (MQ=60)
                                    TCAAAGCCTTTCAGATCGCCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCGCGGAGTTCGCTTTCAGGTATTTGGCGATTTCAGCCG                                                           <  SRR4302543.1111333/94‑1 (MQ=60)
                                     CAAAGCCTTTCAGATCGCCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCGCGGAGTTCGCTTTCAGGTATTTGGCGATTTCAGCCGG                                                          >  SRR4302543.4156717/1‑94 (MQ=60)
                                       AAGCCTTTCAGATCGTCTTTCTCATGCCAGGTCAGCGGTCCCATTACGGTATCCGCGGAGTTCGCTTTCAGGTATTTGGCGATTTCAGCCGGAT                                                        <  SRR4302543.6649285/94‑1 (MQ=60)
                                       AAGCCTTTCAGATCGCCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCGCGGAGTTCGCTTTCAGGTATTTGGCGATTTCAGCCGGAT                                                        <  SRR4302543.4065453/94‑1 (MQ=60)
                                       AAGCCTTTCAGATCGCCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCGCGGAGTTCGCTTTCAGGTATTTGGCGATTTCAGCCGGAT                                                        <  SRR4302543.5731657/94‑1 (MQ=60)
                                        AGCCTTTCAGATCGCCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCGCGGAGTTCGCTTTCAGGTATTTGGCGATTTCAGCCGGATC                                                       <  SRR4302543.7578498/94‑1 (MQ=60)
                                         GCCTTTCAGATCGCCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCGCGGAGTTCGCTTTCAGGTATTTGGCGATTTCAGCCGGATCG                                                      <  SRR4302543.1855026/94‑1 (MQ=60)
                                         GCCTTTCAGATCGCCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCGCGGAGTTCGCTTTCAGGTATTTGGCGATTTCAGCCGGATCG                                                      <  SRR4302543.5777060/94‑1 (MQ=60)
                                          CCTTTCAGATCGCCTTTCTCATCCCAGGTCAGCGGTCCCATTCCGGCATCCGCGGAGTTCGCTTTCAGGTATTTGGCGCTTTCAGCCGGATCGT                                                     >  SRR4302543.3488487/1‑94 (MQ=60)
                                          CCTTTCAGATCGCCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCGCGGAGTTCGCTTTCAGGTATTTGGCGATTTCAGCCGGATCGT                                                     <  SRR4302543.234436/94‑1 (MQ=60)
                                           CTTTCAGATCGCCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCGCGGAGTTCGCTTTCAGGTATTTGGCGATTTCAGCCGGATCGTC                                                    >  SRR4302543.3517755/1‑94 (MQ=60)
                                           CTTTCAGATCGCCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCGCGGAGTTCGCTTTCAGGTATTTGGCGATTTCAGCCGGATCGTC                                                    >  SRR4302543.4046523/1‑94 (MQ=60)
                                           CTTTCAGATCGCCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCGCGGAGTTCGCTTTCAGGTATTTGGCGATTTCAGCCGGATCGTC                                                    >  SRR4302543.888957/1‑94 (MQ=60)
                                            TTTCAGATCGCCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCGCGGAGTTCGCTTTCAGGTATTTGGCGATTTCAGCCGGATCGTCC                                                   >  SRR4302543.5588014/1‑94 (MQ=60)
                                            TTTCAGATCGCCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCGCGGAGTTCGCTTTCAGGTATTTGGCGATTTCAGCCGGATCGTCA                                                   <  SRR4302543.6783438/94‑1 (MQ=60)
                                            TTTCAGATCGCCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCGCGGAGTTCGCTTTCAGGTATTTGGCGATTTCAGCCGGATCGTCA                                                   >  SRR4302543.3291999/1‑94 (MQ=60)
                                             TTCAGATCGCCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCGCGGAGTTCGCTTTCAGGTATTTGGCGATTTCAGCCGGATCGTCAG                                                  <  SRR4302543.3752082/94‑1 (MQ=60)
                                             TTCAGATCGCCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCGCGGAGTTCGCTTTCAGGTATTTGGCGATTTCAGCCGGATCGTCAG                                                  >  SRR4302543.2066252/1‑94 (MQ=60)
                                              TCAGATCGCCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCGCGGAGTTCGCTTTCAGGTATTTGGCGATTTCAGCCGGATCGTCAGA                                                 >  SRR4302543.612114/1‑94 (MQ=60)
                                               gtctcggcattcctgctgaaccgctcttccgatctccactcTATCCGCGGAGTTCGCTTTCAGGTATTTGGCGATTTCAGCCGGATCGTCAGAC                                                <  SRR4302543.511720/53‑1 (MQ=60)
                                               CAGATCGCCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCGCGGAGTTCGCTTTCAGGTATTTGGCGATTTCAGCCGGATCGTCAGAC                                                <  SRR4302543.2770992/94‑1 (MQ=60)
                                               CAGATCGCCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCGCGGAGTTCGCTTTCAGGTATTTGGCGATTTCAGCCGGATCGTCAGAC                                                <  SRR4302543.3980078/94‑1 (MQ=60)
                                                AGATCGCCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCGCGGAGTTCGCTTTCAGGTATTTGGCGATTTCAGCCGGATCGTCAGACT                                               <  SRR4302543.1029839/94‑1 (MQ=60)
                                                AGATCGCCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCGCGGAGTTCGCTTTCAGGTATTTGGCGATTTCAGCCGGATCGTCAGACT                                               >  SRR4302543.4552743/1‑94 (MQ=60)
                                                AGATCGCCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCGCGGAGTTCGCTTTCAGGTATTTGGCGATTTCAGCCGGATCGTCAGACT                                               <  SRR4302543.6185326/94‑1 (MQ=60)
                                                AGATCGCCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCGCGGAGTTCGCTTTCAGGTATTTGGCGATTTCAGCCGGATCGTCAGACT                                               <  SRR4302543.4165123/94‑1 (MQ=60)
                                                AGATCGCCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCGCGGAGTTCGCTTTCAGGTATTTGGCGATTTCAGCCGGATCGTCAGACT                                               >  SRR4302543.1769135/1‑94 (MQ=60)
                                                AGATCGCCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCGCGGAGTTCGCTTTCAGGTATTTGGCGATTTCAGCCGGATCGTCAGACT                                               >  SRR4302543.3196680/1‑94 (MQ=60)
                                                AGATCGCCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCGCGGAGTTCGCTTTCAGGTATTTGGCGATTTCAGCCGGATCGTCAGACT                                               <  SRR4302543.7557852/94‑1 (MQ=60)
                                                 GATCGCCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCGCGGAGTTCGCTTTCAGGTATTTGGCGATTTCAGCCGGATCGTCAGACTG                                              <  SRR4302543.2451900/94‑1 (MQ=60)
                                                 GATCGCCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCGCGGAGTTCGCTTTCAGGTATTTGGCGATTTCAGCCGGATCGTCAGACTG                                              >  SRR4302543.3811276/1‑94 (MQ=60)
                                                  ATCGCCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCGCGGAGTTCGCTTTCAGGTATTTGGCGATTTCAGCCGGATCGTCAGACTGA                                             >  SRR4302543.4700131/1‑94 (MQ=60)
                                                  ATCGCCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCGCGGAGTTCGCTTTCAGGTATTTGGCGATTTCAGCCGGATCGTCAGACTGA                                             <  SRR4302543.115189/94‑1 (MQ=60)
                                                  ATCGCCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGGATCCGCGGAGTTCGCTTTCAGGTATTTGGCGATTTCAGCCGGATCGTCAGACTGA                                             >  SRR4302543.7477692/1‑94 (MQ=60)
                                                   TCGCCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCTCGGAGTTCGCTTTCAGGTATTTGGCGATTTCAGCCGGATCGTCAGACTGAT                                            >  SRR4302543.3780021/1‑94 (MQ=60)
                                                   TCGCCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCGCGGAGTTCGCTTTCAGGTATTTGGCGATTTCAGCCGGATCGTCAGACTGAT                                            <  SRR4302543.1216807/94‑1 (MQ=60)
                                                    CGCCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCGCGGAGTTCGCTTTCAGGTATTTGGCGATTTCAGCCGGATCGTCAGACTGATT                                           <  SRR4302543.6880556/94‑1 (MQ=60)
                                                    CGCCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCGCGGAGTTCGCTTTCAGGTATTTGGCGATTTCAGCCGGATCGTCAGACTGATT                                           >  SRR4302543.3497735/1‑94 (MQ=60)
                                                     GCCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCGCGGAGTTCGCTTTCAGGTATTTGGCGATTTCAGCCGGATCGTCAGACTGATTC                                          <  SRR4302543.5117091/94‑1 (MQ=60)
                                                     GCCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCGCGGAGTTCGCTTTCAGGTATTTGGCGATTTCAGCCGGATCGTCAGACTGATTC                                          <  SRR4302543.3677156/94‑1 (MQ=60)
                                                     GCCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCGCGGAGTTCGCTTTCAGGTATTTGGCGATTTCAGCCGGATCGTCAGACTGATTC                                          <  SRR4302543.2508464/94‑1 (MQ=60)
                                                     GCCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCGCGGAGTTCGCTTTCAGGTATTTGGCGATTTCAGCCGGATCGTCAGACTGATTC                                          <  SRR4302543.503410/94‑1 (MQ=60)
                                                     GCCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCGCGGAGTTCGCTTTCAGGTATTTGGCGATTTCAGCCGGATCGTCAGACTGATTC                                          >  SRR4302543.1288491/1‑94 (MQ=60)
                                                     GCCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCGCGGAGTTCGCTTTCAGGTATTTGGCGATTTCAGCCGGATCGTCAGACTGATTC                                          <  SRR4302543.419365/94‑1 (MQ=60)
                                                     GCCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCGCGGAGTTCGCTTTCAGGTATTTGGCGATTTCAGCCGGATCGTCAGACTGATTC                                          <  SRR4302543.609844/94‑1 (MQ=60)
                                                      CCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCGCGGAGTTCGCTTTCAGGTATTTGGCGATTTCAGCCGGATCGTCAGACTGATTCA                                         <  SRR4302543.6146374/94‑1 (MQ=60)
                                                      CCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCGCGGAGTTCGCTTTCAGGTATTTGGCGATTTCAGCCGGATCGTCAGACTGATTCA                                         <  SRR4302543.3608763/94‑1 (MQ=60)
                                                      CCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGGATCCGCGGAGTTCGCTTTCAGGTATTTGGCGATTTCAGCCGGATCGTCAGACTGATTCA                                         >  SRR4302543.4298165/1‑94 (MQ=60)
                                                       CTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCGCGGAGTTCGCTTTCAGGTATTTGGCGATTTCAGCCGGATCGTCAGACTGATTCAG                                        >  SRR4302543.2423553/1‑94 (MQ=60)
                                                       CTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCGCGGAGTTCGCTTTCAGGTATTTGGCGATTTCAGCCGGATCGTCAGACTGATTCAG                                        <  SRR4302543.7407917/94‑1 (MQ=60)
                                                       CTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCGCGGAGTTCGCTTTCAGGTATTTGGCGATTTCAGCCGGATCGTCAGACTGATTCAG                                        >  SRR4302543.1916462/1‑94 (MQ=60)
                                                       CTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCGCGGAGTTCGCTTTCAGGTATTTGGCGATTTCAGCCGGATCGTCAGACTGATTCAG                                        <  SRR4302543.5618408/94‑1 (MQ=60)
                                                       CTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCGCGGAGTTCGCTTTCAGGTATTTGGCGATTTCAGCCGGATCGTCAGACTGATTCAG                                        <  SRR4302543.3534147/94‑1 (MQ=60)
                                                       CTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCGCGGAGTTCGCTTTCAGGTATTTGGCGATTTCAGCCGGATCGTCAGACTGATTCAG                                        >  SRR4302543.7383835/1‑94 (MQ=60)
                                                       CTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCGCGGAGTTCGCTTTCAGGTATTTGGCGATTTCAGCCGGATCGTCAGACTGATTCAG                                        <  SRR4302543.371566/94‑1 (MQ=60)
                                                       CTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCGCGGAGTTCGCTTTCAGGTATTTGGCGATTTCAGCCGGATCGTCAGACTGATTCAG                                        <  SRR4302543.3812833/94‑1 (MQ=60)
                                                       CTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCGCGGAGTTCGCTTTCAGGTATTTGGCGATTTCAGCCGGATCGTCAGACTGATTCAG                                        >  SRR4302543.6747801/1‑94 (MQ=60)
                                                       CTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCGCGGAGTTCGCTTTCAGGTATTTGGCGATTTCAGCCGGATCGTCAGACTGATTCAG                                        <  SRR4302543.2955509/94‑1 (MQ=60)
                                                       CTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCGCGGAATTCGCTTTCAGGTATTTGGCGATTTCAGCCGGATCGTCAGACTGATTCAG                                        <  SRR4302543.1036667/94‑1 (MQ=60)
                                                        TTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCGCGGAGTTCGCTTTCAGGTATTTGGCGATTTCAGCCGGATCGTCAGACTGATTCAGG                                       <  SRR4302543.2462385/94‑1 (MQ=60)
                                                        TTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCGCGGAGTTCGCTTTCAGGTATTTGGCGATTTCAGCCGGATCGTCAGACTGATTCAGG                                       <  SRR4302543.2758281/94‑1 (MQ=60)
                                                          TCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCGCGGAGTTCGCTTTCAGGTATTTGGCGATTTCAGCCGGATCGTCAGACTGATTCAGGCC                                     <  SRR4302543.70253/94‑1 (MQ=60)
                                                           CTCATCCCAGGTCAGCGGTCCCATTACGGTATCCGCGGAGTTCGCTTTCAGGTATTTGGCGATTTCAGCCGGATCGTCAGACTGATTCAGGCCC                                    <  SRR4302543.2500533/94‑1 (MQ=60)
                                                           CTCATCCCAGGTCAGCGGTCCCATTACGGTATCCGCGGAGTTCGCTTTCAGGTATTTGGCGATTTCAGCCGGATCGTCAGACTGATTCAGGCCC                                    <  SRR4302543.3129405/94‑1 (MQ=60)
                                                             CATCCCAGGTCAGCGGTCCCATTACGGTATCCGCGGAGTTCGCTTTCAGGTATTTGGCGATTTCAGCCGGATCGTCAGACTGATTCAGGCCCGC                                  >  SRR4302543.2364031/1‑94 (MQ=60)
                                                              ATCCCAGGTCAGCGGTCCCATTACGGTATCCGCGGAGTTCGCTTTCAGGTATTTGGCGATTTCAGCCGGATCGTCAGACTGATTCAGGCCCGCC                                 >  SRR4302543.3695537/1‑94 (MQ=60)
                                                              ATCCCAGGTCAGCGGCCCCATTACGGGATCAGCGGAGTTCGCTTTCAGGTATTTGGCGATTTCAGCCGCATCGGCAGACTGATTCAGGCCCGCC                                 >  SRR4302543.6894559/1‑94 (MQ=60)
                                                               TCCCAGGTCAGCGGTCCCATTACGGTATCCGCGGAGTTCGCTTTCAGGTATTTGGCGATTTCAGCCGGATCGTCAGACTGATTCAGGCCCGCCT                                >  SRR4302543.5178765/1‑94 (MQ=60)
                                                               TCCCAGGTCAGCGGTCCCATTACGGTATCCGCGGAGTTCGCTTTCAGGTATTTGGCGATTTCAGCCGGATCGTCAGACTGATTCAGGCCCGCCT                                <  SRR4302543.1699536/94‑1 (MQ=60)
                                                               TCCCAGGTCAGCGGTCCCATTACGGTATCCGCGGAGTTCGCTTTCAGGTATTTGGCGATTTCAGCCGGATCGTCAGACTGATTCAGGCCCGCCT                                <  SRR4302543.419174/94‑1 (MQ=60)
                                                                CCCAGGTCAGCGGTCCCATTACGGTATCCGCGGAGTTCGCTTTCAGGTATTTGGCGATTTCAGCCGGATCGTCAGACTGATTCAGGCCCGCCTG                               <  SRR4302543.7374111/94‑1 (MQ=60)
                                                                CCCAGGTCAGCGGTCCCATTACGGTATCCGCGGAGTTCGCTTTCAGGTATTTGGCGATTTCAGCCGGATCGTCAGACTGATTCAGGCCCGCCTG                               >  SRR4302543.4904823/1‑94 (MQ=60)
                                                                CCCAGGTCAGCGGTCCCATTACGGTATCCGCGGAGTTCGCTTTCAGGTATTTGGCGATTTCAGCCGGATCGTCAGACTGATTCAGGCCCGCCTG                               >  SRR4302543.1029064/1‑94 (MQ=60)
                                                                 CCAGGTCAGCGGTCCCATTACGGTATCCGCGGAGTTCGCTTTCAGGTATTTGGCGATTTCAGCCGGATCGTCAGACTGATTCAGGCCCGCCTGC                              >  SRR4302543.3874631/1‑94 (MQ=60)
                                                                 CCAGGTCAGCGGTCCCATTACGGTATCCGCGGAGTTCGCTTTCAGGTATTTGGCGATTTCAGCCGGATCGTCAGACTGATTCAGGCCCGCCTGC                              >  SRR4302543.387162/1‑94 (MQ=60)
                                                                 CCAGGTCAGCGGTCCCATTACGGTATCCGCGGAGTTCGCTTTCAGGTATTTGGCGATTTCAGCCGGATCGTCAGACTGATTCAGGCCCGCCTGC                              >  SRR4302543.4386300/1‑94 (MQ=60)
                                                                  CAGGTCAGCGGTCCCATTACGGTATCCGCGGAGTTCGCTTTCAGGTATTTGGCGATTTCAGCCGGATCGTCAGACTGATTCAGGCCCGCCTGCA                             <  SRR4302543.1020554/94‑1 (MQ=60)
                                                                  CAGGTCAGCGGTCCCATTACGGTATCCGCGGAGTTCGCTTTCAGGTATTTGGCGATTTCAGCCGGATCGTCAGACTGATTCAGGCCCGCCTGCA                             >  SRR4302543.2971267/1‑94 (MQ=60)
                                                                   AGGTCAGCGGTCCCATTACGGTATCCGCGGAGTTCGCTTTCAGGTATTTGGCGATTTCAGCCGGATCGTCAGACTGATTCAGGCCCGCCTGCAA                            <  SRR4302543.4981546/94‑1 (MQ=60)
                                                                   AGGTCAGCGGTCCCATTACGGTATCCGCGGAGTTCGCTTTCAGGTATTTGGCGATTTCAGCCGGATCGTCAGACTGATTCAGGCCCGCCTGCAA                            >  SRR4302543.6625622/1‑94 (MQ=60)
                                                                   AGGTCAGCGGTCCCATTACGGTATCCGCGGAGTTCGCTTTCAGGTATTTGGCGATTTCAGCCGGATCGTCAGACTGATTCAGGCCCGCCTGCAA                            <  SRR4302543.3330327/94‑1 (MQ=60)
                                                                    GGTCAGCGGTCCCATTACGGTATCCGCGGAGTTCGCTTTCAGGTATTTGGCGATTTCAGCCGGATCGTCAGACTGATTCAGGCCCGCCTGCAAA                           >  SRR4302543.7988994/1‑94 (MQ=60)
                                                                    GGTCAGCGGTCCCATTACGGTATCCGCGGAGTTCGCTTTCAGGTATTTGGCGATTTCAGCCGGATCGTCAGACTGATTCAGGCCCGCCTGCAAA                           <  SRR4302543.1803166/94‑1 (MQ=60)
                                                                      TCAGCGGTCCCATTACGGTATCCGCGGAGTTCGCTTTCAGGTATTTGGCGATTTCAGCCGAATCGTCAGACTGATTCAGGCCCGCCTGCAAAGA                         <  SRR4302543.2871212/94‑1 (MQ=60)
                                                                       CAGCGGTCCCATTACGGTATCCGCGGAGTTCGCTTTCAGGTATTTGGCGATTTCAGCCGGATCGTCAGACTGATTCAGGCCCGCCTGCAAAGAT                        <  SRR4302543.6938729/94‑1 (MQ=60)
                                                                       CAGCGGTCCCATTACGGTATCCGCGGAGTTCGCTTTCAGGTATTTGGCGATTTCAGCCGGATCGTCAGACTGATTCAGGCCCGCCTGCAAAGAT                        <  SRR4302543.935174/94‑1 (MQ=60)
                                                                       CAGCGGTCCCATTACGGTATCCGCGGAGTTCGCTTTCAGGTATTTGGCGATTTCAGCCGGATCGTCAGACTGATTCAGGCCCGCCTGCAAAGAT                        >  SRR4302543.789755/1‑94 (MQ=60)
                                                                       CAGCGGTCCCATTACGGTATCCGCGGAGTTCGCTTTCAGGTATTTGGCGATTTCAGCCGGATCGTCAGACTGATTCAGGCCCGCCTGCAAAGAT                        <  SRR4302543.4120293/94‑1 (MQ=60)
                                                                        AGCGGTCCCATTACGGTATCCGCGGAGTTCGCTTTCAGGTATTTGGCGATTTCAGCCGGATCGTCAGACTGATTCAGGCCCGCCTGCAAAGATT                       <  SRR4302543.631947/94‑1 (MQ=60)
                                                                           GGTCCCATTACGGTATCCGCGGAGTTTGCTTTCAGGTATTTGGCGATTTCAGCCGGATCGTCAGACTGATTCAGGCCCGCCTGCAAAGATCGCA                    >  SRR4302543.1723515/1‑94 (MQ=60)
                                                                           GGTCCCATTACGGTATCCGCGGAGTTCGCTTTCAGGTATTTGGCGATTTCAGCCGGATCGTCAGACTGATTCAGGCCCGCCTGCAAAGATTGCA                    >  SRR4302543.2575103/1‑94 (MQ=60)
                                                                            GTCCCATTACGGTATCCGCGGAGTTCGCTTTCAGGTATTTGGCGATTTCAGCCGGATCGTCAGACTGATTCAGGCCCGCCTGCAAAGATTGCAG                   <  SRR4302543.3833451/94‑1 (MQ=60)
                                                                            GTCCCATTACGGTATCCGCGGAGTTCGCTTTCAGGTATTTGGCGATTTCAGCCGGATCGTCAGACTGATTCAGGCCCGCCTGCAAAGATTGCAG                   <  SRR4302543.7133544/94‑1 (MQ=60)
                                                                            GTCCCATTACGGTATCCGCGGAGTTCGCTTTCAGGTATTTGGCGATTTCAGCCGGATCGTCAGACTGATTCAGGCCCGCCTGCAAAGATTGCAG                   <  SRR4302543.5684184/94‑1 (MQ=60)
                                                                            GTCCCATTACGGTATCCGCGGAGTTCGCTTTCAGGTATTTGGCGATTTAAGCCGGATCGTCAGACTGATTCAGGCCCGCCTGCAAAGATTGCAG                   <  SRR4302543.5666046/94‑1 (MQ=60)
                                                                            GTCCCA‑TACGGTATCCGCGGAGTTCGCTTTCAGGTATTCGGCGATTTCAGCCGGATCGTCAGACTGATTCAGGCCCGCCTGCAAAGATTGCAGC                  <  SRR4302543.1174571/94‑1 (MQ=60)
                                                                             TCCCATTACGGTATCCGCGGAGTTCGCTTTCAGGTATTTGGCGATTTCAGCCGGATCGTCAGACTGATTCAGGCCCGCCTGCAAAGATTGCAGC                  >  SRR4302543.2943963/1‑94 (MQ=60)
                                                                              CCCATTACGGTATCCGCGGAGTTCGCTTTCAGGTATTTGGCGATTTCAGCCGGATCGTCAGACTGATTCAGGCCCGCCTGCAAAGATTGCAGCG                 >  SRR4302543.4646192/1‑94 (MQ=60)
                                                                              CCCATTACGGTATCCGCGGAGTTCGCTTTCAGGTATTTGGCGATTTCAGCCGGATCGTCAGACTGATTCAGGCCCGCCTGCAAAGATTGCAGCG                 <  SRR4302543.7117377/94‑1 (MQ=60)
                                                                               CCATTACGGTATCCGCGGAGTTCGCTTTCAGGTATTTGGCGATTTCAGCCGGATCGTCAGACTGATTCAGGCGCGCCTGCAGAGAGTGCAGCGG                >  SRR4302543.4609186/1‑94 (MQ=60)
                                                                               CCATTACGGTATCCGCGGAGTTCGCTTTCAGGTATTTGGCGATTTCAGCCGGATCGTCAGACTGATTCAGGCCCGCCTGCAAAGATTGCAGCGC                >  SRR4302543.1195015/1‑94 (MQ=60)
                                                                                CATTACGGTATCCGCGGAGTTCGCTTTCAGGTATTTGGCGATTTCAGCCGGATCGTCAGACTGATTCAGGCCCGCCTGCAAAGATTGCAGCGCG               >  SRR4302543.1767695/1‑94 (MQ=60)
                                                                                CATTACGGTATCCGCGGAGTTCGCTTTCAGGTATTTGGCGATTTCAGCCGGATCGTCAGACTGATTCAGGCCCGCCTGCAAAGATTGCAGCGCG               <  SRR4302543.753379/94‑1 (MQ=60)
                                                                                CATTACGGTATCCGCGGAGTTCGCTTTCAGGTATTTGGCGATTTCAGCCGGATCGTCAGACTGATTCAGGCCCGCCTGCAAAGATTGCAGCGCG               <  SRR4302543.301100/94‑1 (MQ=60)
                                                                                CATTACGGTATCCGCGGAGTTCGCTTTCAGGTATTTGGCGATTTCAGCCGGATCGTCAGACTGATTCAGGCCCGCCTGCAAAGATTGCAGCGCG               <  SRR4302543.4395367/94‑1 (MQ=60)
                                                                                CATTACGGTATCCGCGGAGTTCGCTTTCAGGTATTTGGCGATTTCAGCCGGATCGTCAGACTGATTCACTGCCGCCTGCAAAGATTGCAGCGCG               >  SRR4302543.4502257/1‑94 (MQ=60)
                                                                                CATTACGGTATCCGCGGAGGTCGCTTTCAGGTATTTGGCGATTTCAGCCGGATCGTCAGACTGATTCAGGCCCGCCTGCAAAGATTGCAGCGCG               <  SRR4302543.1954475/94‑1 (MQ=60)
                                                                                 ATTACGGTATCCGCGGAGTTCGCTTTCAGGTATTTGGCGATTTCAGCCGGATCGTCAGACTGATTCAGGCCCGCCTGCAAAGATTGCAGCGCGG              >  SRR4302543.5643592/1‑94 (MQ=60)
                                                                                  TTACGGTATCCGCGGAGTTCGCTTTCAGGTATTTGGCGATTTCAGCCGGATCGTCAGACTGATTCAGGCCCGCCTGCAAAGATTGCAGCGCGGC             >  SRR4302543.3776617/1‑94 (MQ=60)
                                                                                  TTACGGTATCCGCGGAGTTCGCTTTCAGGTATTTGGCGATTTCAGCCGGATCGTCAGACTGATTCAGGCCCGCCTGCAAAGATTGCAGCGCGGC             >  SRR4302543.6655534/1‑94 (MQ=60)
                                                                                   TACGGTATCCGCGGAGTTCGCTTTCAGGTATTTGGCGATTTCAGCCGGATCGTCAGACTGATTCAGGCCCGCCTGCAAAGATTGCAGCGCGGCG            <  SRR4302543.3975558/94‑1 (MQ=60)
                                                                                   TACGGTATCCGCGGAGTTCGCTTTCAGGTATTTGGCGATTTCAGCCGGATCGTCAGACTGATTCAGGCCCGCCTGCAAAGATTGCAGCGCGGCG            <  SRR4302543.3757783/94‑1 (MQ=60)
                                                                                   TACGGTATCCGCGGAGTTCGCTTTCAGGTATTTGGCGATTTCAGCCGGATCGTCAGACTGATTCAGGCCCGCCTGCAAAGATTGCAGCGCGGCG            >  SRR4302543.7973943/1‑94 (MQ=60)
                                                                                    ACGGTATCCGCGGAGTTCGCTTTCAGGTATTTGGCGATTTCAGCCGGATCGTCAGACTGATTCAGGCCCGCCTGCAAAGATTGCAGCGCGGCGT           <  SRR4302543.4834495/94‑1 (MQ=60)
                                                                                    ACGGTATCCGCGGAGTTCGCTTTCAGGTATTTGGCGATTTCAGCCGGATCGTCAGACTGATTCAGGCCCGACTGCAAAGATTGCAGCGCGGCGT           >  SRR4302543.1878429/1‑94 (MQ=60)
                                                                                       GTATCCGCGGAGTTCGCTTTCAGGTATTTGGCGATTTCAGCCGGATCGTCAGACTGATTCAGGCCCGCCTGCAAAGATTGCAGCGCGGCGTAGG        >  SRR4302543.3882584/1‑94 (MQ=60)
                                                                                        TATCCGCGGAGTTCGCTTTCAGGTATTTGGCGATTTCAGCCGGATCGTCAGACTGATTCAGGCCCGCCTGCAAAGATTGCAGCGCGGCGTAGGT       <  SRR4302543.4868683/94‑1 (MQ=60)
                                                                                         ATCCGCGGAGTTCGCTTTCAGGTATTTGGCGATTTCAGCCGGATCGTCAGACTGATTCAGGCCCGCCTGCAAAGATTGCAGCGCGGCGTAGGTG      <  SRR4302543.3622886/94‑1 (MQ=60)
                                                                                         ATCCGCGGAGTTCGCTTTCAGGTATTTGGCGATTTCAGCCGGATCGTCAGACTGATTCAGGCCCGCCTGCAAAGATTGCAGCGCGGCGTAGGTG      >  SRR4302543.4373342/1‑94 (MQ=60)
                                                                                         ATCCGCGGAGTTCGCTTTCAGGTATTTGGCGATTTCAGCCGGATCGTCAGACTGATTCAGGCCCGCCTGCAAAGATTGCAGCGCGGCGTAGGTG      >  SRR4302543.544840/1‑94 (MQ=60)
                                                                                         ATCCGCGGAGTTCGCTTTCAGGGATTTGGCGATTTCAGCCGGATCGTCAGACTGATTCAGGCCCGCCTGCAAAGATTGCAGCGCGGCGTAGGTG      >  SRR4302543.1382361/1‑94 (MQ=60)
                                                                                           CCGCGGAGTTCGCTTTCAGGTGTTTGGCGATTTCAGCCGGATCGTCAGACTGATTCAGGCCCGCCTGCAAAGATTGCAGCGCGGCGTAGGTGGT    <  SRR4302543.1831795/94‑1 (MQ=60)
                                                                                           CCGCGGAGTTCGCTTTCAGGTATTTGGCGATTTCAGCCGGATCGTCAGACTGATTCAGGCCCGCCTGCAAAGATTGCAGCGCGGCGTAGGTGGT    <  SRR4302543.6915731/94‑1 (MQ=60)
                                                                                           CCGCGGAGTTCGCTTTCAGGTATTGGGCGATTTCAGCCGGATCGTCAGACTGATTCAGGCCCGCCTGCAAAGATTGCAGCGCGGCGTAGGTGGT    <  SRR4302543.1560755/94‑1 (MQ=60)
                                                                                            CGCGGAGTTCGCTTTCAGGTATTTGGCGATTTCAGCCGGATCGTCAGACTGATTCAGGCCCGCCTGCAAAGATTGCAGCGCGGCGTAGGTGGTC   >  SRR4302543.2273278/1‑94 (MQ=60)
                                                                                             GCGGAGTTCGCTTTCAGGTATTTGGCGATTTCAGCCGGATCGTCAGACTGATTCAGGCCCGCCTGCAAAGATTGCAGCGCGGCGTAGGTGGTCC  <  SRR4302543.148771/94‑1 (MQ=60)
                                                                                             |                                                                                             
GCCGTGCCGTTGGCGTGCCAGTCAAATACGCCGAACTCAAAGCCTTTCAGATCGCCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCACGGAGTTCGCTTTCAGGTATTTGGCGATTTCAGCCGGATCGTCAGACTGATTCAGGCCCGCCTGCAAAGATTGCAGCGCGGCGTAGGTGGTCC  >  NC_000913/3596202‑3596388

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 9 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑