Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 1696 279.3 15874187 97.8% 15524954 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,725,633 T→C L456L (CTA→CTG)  xylB ← xylulokinase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,725,6330TC99.6% 806.8 / ‑5.5 254L456L (CTA→CTG) xylBxylulokinase
Reads supporting (aligned to +/- strand):  ref base T (1/0);  new base C (197/55);  total (198/56)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.82e-01

Only 100 of 264 total aligned reads displayed.

GTTTACGCCATTAATGGCAGAAGTTGCTGATAGAGGCGACGGAACGTTTCTCGTCGTGGCTGATAAGCGGCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCC  >  NC_000913/3725544‑3725722
                                                                                         |                                                                                         
gTTTACGCCATTAATGGCAGAAGTTGCTGATAGAGGCGACGGAACGTTTCTCGTCGTGGCTGATAAGCGGCATAACGCTGCGCATCTGGCAGa                                                                                        >  1:6569026/1‑93 (MQ=255)
 tttACGCCATTAATGGCAGAAGTTGCTGATAGAGGCGACGGAACGTTTCTCGTCGTGGCTGATAAGCGGCATAACGCTGCGCATCTGGCAGAt                                                                                       >  2:7008636/1‑93 (MQ=255)
 tttACGCCATTAATGGCAGAAGTTGCTGATAGAGGCGACGGAACGTTTCTCGTCGTGGCTGATAAGCGGCATAACGCTGCGCATCTGGCAGAt                                                                                       >  2:6255482/1‑93 (MQ=255)
 tttACGCCATTAATGGCAGAAGTTGCTGATAGAGGCGACGGAACGTTTCTCGTCGTGGCTGATAAGCGGCATAACGCTGCGCATCTGGCAGAt                                                                                       >  1:6772076/1‑93 (MQ=255)
  ttACGCCATTAATGGCAGAAGTTGCTGATAGAGGCGACGGAACGTTTCTCGTCGTGGCTGATAAGCGGCATAACGCTGCGCATCTGGCAGCTg                                                                                      >  2:2676625/1‑93 (MQ=255)
  ttACGCCATTAATGGCAGAAGTTGCTGATAGAGGCGACGGAACGTTTCTCGTCGTGGCTGATAAGCGGCATAACGCTGCGCATCTGGCAGATg                                                                                      >  2:2353091/1‑93 (MQ=255)
     cGCCATTAATGGCAGAAGTTGCTGATAGAGGCGACGGAACGTTTCTCGTCGTGGCTGATAAGCGGCATAACGCTGCGCATCTGGCAGATGCGa                                                                                   >  1:4714139/1‑93 (MQ=255)
     cGCCATTAATGGCAGAAGTTGCTGATAGAGGCGACGGAACGTTTCTCGTCGTGGCTGATAAGCGGCATAACGCTGCGCATCTGGCAGATGCGa                                                                                   >  2:3131922/1‑93 (MQ=255)
      gCCATTAATGGCAGAAGTTGCTGATAGAGGCGACGGAACGTTTCTCGTCGTGGCTGATAAGCGGCATAACGCTGCGCATCTGGCAGATGCGAc                                                                                  >  2:4107332/1‑93 (MQ=255)
         aTTAATGGCAGAAGTTGCTGATAGAGGCGACGGAACGTTTCTCGTCGTGGCTGATAAGCGGCATAACGCTGCGCATCTGGCAGATGCGACTGt                                                                               >  1:7038278/1‑93 (MQ=255)
         aTTAATGGCAGAAGTTGCTGATAGAGGCGACGGAACGTTTCTCGTCGTGGCTGATAAGCGGCATAACGCTGCGCATCTGGCAGATGCGACTGt                                                                               >  1:3006526/1‑93 (MQ=255)
          ttAATGGCAGAAGTTGCTGATAGAGGCGACGGAACGTTTCTCGTCGTGGCTGATAAGCGGCATAACGCTGCGCATCTGGCAGATGCGACTGtt                                                                              >  2:5510936/1‑93 (MQ=255)
          ttAATGGCAGAAGTTGCTGATAGAGGCGACGGAACGTTTCTCGTCGTGGCTGATAAGCGGCATAACGCTGCGCATCTGGCAGATGCGACTGtt                                                                              >  1:4143767/1‑93 (MQ=255)
           tAATGGCAGAAGTTGCTGATAGAGGCGACGGAACGTTTCTCGTCGTGGCTGATAAGCGGCATAACGCTGCGCATCTGGCAGATGCGACTGTTc                                                                             >  2:7376060/1‑93 (MQ=255)
             aTGGCAGAAGTTGCTGATAGAGGCGACGGAACGTTTCTCGTCGTGGCTGATAAGCGGCATAACGCTGCGCATCTGGCAGATGCGACTGTTCTa                                                                           >  1:2388920/1‑93 (MQ=255)
              tGGCAGAAGTTGCTGATAGAGGCGACGGAACGTTTCTCGTCGTGGCTGATAAGCGGCATAACGCTGCGCATCTGGCAGATGCGACTGTTCTaa                                                                          >  1:7300981/1‑93 (MQ=255)
              tGGCAGAAGTTGCTGATAGAGGCGACGGAACGTTTCTCGTCGTGGCTGATAAGCGGCATAACGCTGCGCATCTGGCAGATGCGACTGTTCTaa                                                                          >  1:3209809/1‑93 (MQ=255)
                 cAGAAGTTGCTGATAGAGGCGACGGAACGTTTCTCGTCGTGGCTGATAAGCGGCATAACGCTGCGCATCTGGCAGATGCGACTGTTCTAACgg                                                                       >  1:3857568/1‑93 (MQ=255)
                  aGAAGTTGCTGATAGAGGCGACGGAACGTTTCTCGTCGTGGCTGATAAGCGGCATAACGCTGCGCATCTGGCAGATGCGACTGTTCTAACGGt                                                                      >  1:1749985/1‑93 (MQ=255)
                    aaGTTGCTGATAGAGGCGACGGAACGTTTCTCGTCGTGGCTGATAAGCGGCATAACGCTGCGCATCTGGCAGATGCGACTGTTCTAACGGTAg                                                                    <  2:2535385/93‑1 (MQ=255)
                     aGTTGCTGATAGAGGCGACGGAACGTTTCTCGTCGTGGCTGATAAGCGGCATAACGCTGCGCATCTGGCAGATGCGACTGTTCTAACGGTAGt                                                                   >  2:1094060/1‑93 (MQ=255)
                     aGTTGCTGATAGAGGCGACGGAACGTTTCTCGTCGTGGCTGATAAGCGGCATAACGCTGCGCATCTGGCAGATGCGACTGTTCTAACGGTAGt                                                                   <  1:395709/93‑1 (MQ=255)
                       ttGCTGATAGAGGCGACGGAACGTTTCTCGTCGTGGCTGATAAGCGGCATAACGCTGCGCATCTGGCAGATGCGACTGTTCTAACGGTAGTTg                                                                 >  1:1616303/1‑93 (MQ=255)
                       ttGCTGATAGAGGCGACGGAACGTTTCTCGTCGTGGCTGATAAGCGGCATAACGCTGCGCATCTGGCAGATGCGACTGTTCTAACGGTAGTTg                                                                 >  2:530647/1‑93 (MQ=255)
                       ttGCTGATAGAGGCGACGGAACGTTTCTCGTCGTGGCTGATAAGCGGCATAACGCTGCGCATCTGGCAGATGCGACTGTTCTAACGGTAGTTg                                                                 >  1:6130001/1‑93 (MQ=255)
                       ttGCTGATAGAGGCGACGGAACGTTTCTCGTCGTGGCTGATAAGCGGCATAACGCTGCGCATCTGGCAGATGCGACTGTTCTAACGGTAGTTg                                                                 >  2:4974010/1‑93 (MQ=255)
                        tGCTGATAGAGGCGACGGAACGTTTCTCGTCGTGGCTGATAAGCGGCATAACGCTGCGCATCTGGCAGATGCGACTGTTCTAACGGTAGTTGc                                                                <  1:7174126/93‑1 (MQ=255)
                         gCTGATAGAGGCGACGGAACGTTTCTCGTCGTGGCTGATAAGCGGCATAACGCTGCGCATCTGGCAGATGCGACTGTTCTAACGGTAGTTGCg                                                               >  1:354213/1‑93 (MQ=255)
                          cTGATAGAGGCGACGGAACGTTTCTCGTCGGGGCTGATAAGCGGCATAACGCTGCGCAACTGGCAGATGCGACTGTTCTAACTGTAGTTGCgg                                                              >  1:7631449/1‑93 (MQ=255)
                            gATAGAGGCGACGGAACGTTTCTCGTCGTGGCTGATAAGCGGCATAACGCTGCGCATCTGGCAGATGCGACTGTTCTAACGGTAGTTGCGGca                                                            >  1:5356666/1‑93 (MQ=255)
                             aTAGAGGCGACGGAACGTTTCTCGTCGTGGCTGATAAGCGGCATAACGCTGCGCATCTGGCAGATGCGACTGTTCTAACGGTAGTTGCGGcaa                                                           >  2:6711245/1‑93 (MQ=255)
                             aTAGAGGCGACGGAACGTTTCTCGTCGTGGCTGATAAGCGGCATAACGCTGCGCATCTGGCAGATGCGACTGTTCTAACGGTAGTTGCGGcaa                                                           <  1:6403371/93‑1 (MQ=255)
                             aTAGAGGCGACGGAACGTTTCTCGTCGTGGCTGATAAGCGGCATAACGCTGAGCATCTGGCAGATGCGACTGTTCTAACGGTAGTTGCGGcaa                                                           >  2:3763312/1‑93 (MQ=255)
                              tAGAGGCGACGGAACGTTTCTCGTCGTGGCTGATAAGCGGCATAACGCTGCGCATCTGGCAGATGCGACTGTTCTAACGGTAGTTGGGGcaac                                                          >  2:3620081/1‑93 (MQ=255)
                              tAGAGGCGACGGAACGTTTCTCGTCGTGGCTGATAAGCGGCATAACGCTGCGCATCTGGCAGATGCGACTGTTCTAACGGTAGTTGCGGcaac                                                          >  2:5730521/1‑93 (MQ=255)
                              tAGAGGCGACGGAACGTTTCTCGTCGTGGCTGATAAGCGGCATAACGCTGCGCATCTGGCAGATGCGACTGTTCTAACGGTAGTTGCGGcaac                                                          >  1:1577449/1‑93 (MQ=255)
                               agagGCGACGGAACGTTTCTCGTCGTGGCTGATAAGCGGCATAACGCTGCGCATCTGGCAGATGCGACTGTTCTAACGGTAGTTGCGGcaaca                                                         >  1:5618761/1‑93 (MQ=255)
                                gagGCGACGGAACGTTTCTCGTCGTGGCTGATAAGCGGCATAACGCTGCGCATCTGGCAGATGCGACTGTTCTAACGGTAGTTGCGGcaacaa                                                        >  2:7789120/1‑93 (MQ=255)
                                gagGCGACGGAACGTTTCTCGTCGTGGCTGATAAGCGGCATAACGCTGCGCATCTGGCAGATGCGACTGTTCTAACGGTAGTTGCGGcaacaa                                                        >  1:7023831/1‑93 (MQ=255)
                                  ggCGACGGAACGTTTCTCGTCGTGGCTGATAAGCGGCATAACGCTGCGCATCTGGCAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAAtt                                                      >  1:1355572/1‑93 (MQ=255)
                                      gcGGAACGTTTCTCGTCGTGGCTGATAAGCGGCATAACGCTGCGCATCTGGCAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAAt                                                  <  1:6765509/92‑1 (MQ=255)
                                      aCGGAACGTTTCTCGTCGTGGCTGATAAGCGGCATAACGCTGCGCATCTGGCAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAAt                                                  >  2:3549031/1‑93 (MQ=255)
                                       cGGAACGTTTCTCGTCGTGGCTGATAAGCGGCATAACGCTGCGCATCTGGCAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATg                                                 >  2:7847128/1‑93 (MQ=255)
                                        ggAACGTTTCTCGTCGTGGCTGATAAGCGGCATAACGCTGCGCATCTGGCAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGa                                                >  1:4986377/1‑93 (MQ=255)
                                        ggAACGTTTCTCGTCGTGGCTGATAAGCGGCATAACGCTGCGCATCTGGCAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGa                                                >  1:3355663/1‑93 (MQ=255)
                                         gAACGTTTCTCGTCGTGGCTGATAAGCGGCATAACGCTGCGCATCTGGCAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAg                                               >  1:5539559/1‑93 (MQ=255)
                                         gAACGTTTCTCGTCGTGGCTGATAAGCGGCATAACGCTGCGCATCTGGCAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAg                                               <  2:1801612/93‑1 (MQ=255)
                                         gAACGTTTCTCGTCGTGGCTGATAAGCGGCATAACGCTGCGCATCTGGCAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAg                                               >  2:4711475/1‑93 (MQ=255)
                                         gAACGTTTCTCGTCGTGGCTGATAAGCGGCATAACGCTGCGCATCTGGCAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAg                                               >  2:2303338/1‑93 (MQ=255)
                                         gAACGTTTCTCGTCGTGGCTGATAAGCGGCATAACGCTGCGCATCTGGCAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAg                                               >  1:5722230/1‑93 (MQ=255)
                                         gAACGTTTCTCGTCGTGGCTGATAAGCGGAATAACGCTGCGCATCTGGCAGAGGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAg                                               >  1:1127029/1‑93 (MQ=255)
                                          aaCGTTTCTCGTCGTGGCTGATAAGCGGCATAACGCTGCGCATCTGGCAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGc                                              >  2:2814282/1‑93 (MQ=255)
                                            cGTTTCTCGTCGTGGCTGATAAGCGGCATAACGCTGCGCATCTGGCAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGa                                            >  1:5375809/1‑93 (MQ=255)
                                             gTTTCTCGTCGTGGCTGATAAGCGGCATAACGCTGCGCATCTGGCAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGAt                                           >  1:5046187/1‑93 (MQ=255)
                                              tttCTCGTCGTGGCTGATAAGCGGCATAACGCTGCGCATCTGGCAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTTAATGAGCGAtt                                          >  2:5594461/1‑93 (MQ=255)
                                              tttCTCGTCGTGGCTGATAAGCGGCATAACGCTGCGCATCTGGCAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGAtt                                          >  2:3852535/1‑93 (MQ=255)
                                              tttCTCGTCGTGGCTGATAAGCGGCATAACGCTGCGCATCTGGCAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGAtt                                          >  2:7488301/1‑93 (MQ=255)
                                              tttCTCGTCGTGGCTGATAAGCGGCATAACGCTGCGCATCTGGCAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGAtt                                          <  1:6050160/93‑1 (MQ=255)
                                              tttCTCGTCGTGGCTGATAAGCGGCATAACGCTGCGCATCTGGCAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGAtt                                          >  2:2979144/1‑93 (MQ=255)
                                                  tcgtcgTGGCTGATAAGCGGCATAACGCTGCGCATCTGGCAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTtctc                                      >  2:1589306/1‑93 (MQ=255)
                                                  tcgtcgTGGCTGATAAGCGGCATAACGCTGCGCATCTGGCAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTtctc                                      >  1:243312/1‑93 (MQ=255)
                                                   cgtcgtGGCTGATAAGCGGCATAACGCTGCGCATCTGGCAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTctct                                     >  2:6847076/1‑93 (MQ=255)
                                                    gtcgtGGCTGATAAGCGGCATAACGCTGCGCATCTGGCAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTg                                    <  2:606507/93‑1 (MQ=255)
                                                     tcgtGGCTGATAAGCGGCATAACGCTGCGCATCTGGCAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTgg                                   >  2:275138/1‑93 (MQ=255)
                                                      cgtGGCTGATAAGCGGCATAACGCTGCGCATCTGGCAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGa                                  <  1:3102139/93‑1 (MQ=255)
                                                      cgtGGCTGATAAGCGGCATAACGCTGCGCATCTGGCAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGa                                  >  2:1983061/1‑93 (MQ=255)
                                                       gtGGCTGATAAGCGGCATAACGCTGCGCATCTGGCAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGAt                                 >  1:4625615/1‑93 (MQ=255)
                                                       gtGGCTGATAAGCGGCATAACGCTGCGCATCTGGCAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGAt                                 >  1:6642629/1‑93 (MQ=255)
                                                       gtGGCTGATAAGCGGCATAACGCTGCGCATCTGGCAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATCAGCGAATTTTCTGGAt                                 >  1:1014107/1‑93 (MQ=255)
                                                        tGGCTGATAAGCGGCATAACGCTGCGCATCTGGCAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGAtt                                >  2:2803859/1‑93 (MQ=255)
                                                         ggCTGATAAGCGGCATAACGCTGCGCATCTGGCAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTc                               <  1:4487602/93‑1 (MQ=255)
                                                         ggCTGATAAGCGGCATAACGCTGCGCATCTGGCAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTc                               <  2:5881821/93‑1 (MQ=255)
                                                          gCTGATAAGCGGCATAACGCTGCGCATCTGGCAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTcg                              >  2:5906447/1‑93 (MQ=255)
                                                          gCTGATAAGCGGCATAACGCTGCGCATCTGGCAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTcg                              >  2:4257243/1‑93 (MQ=255)
                                                          gCTGATAAGCGGCATAACGCTGCGCATCTGGCAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTcg                              <  2:4462077/93‑1 (MQ=255)
                                                          gCTGATAAGCGGCATAACGCTGCGCATCTGGCAGATGCGACTGTTCTAACGGTAGTCACGGCAACCATTCAATAAGCGATTTCTCTGGAttac                              >  1:2641984/1‑91 (MQ=255)
                                                           cTGATAAGCGGCATAACGCTGCGCATCTGGCAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGGGATTTCTCTGGATTcgc                             >  2:168488/1‑93 (MQ=255)
                                                            tGATAAGCGGCATAACGATGCGCATCTGGTAGATGCGACTGCTCTAACGGGAGTTAAGGAAACAATTGAATGAGCGCTTTCTCAGGAGTcgca                            >  2:5096809/1‑92 (MQ=255)
                                                             gATAAGCGGCATAACGCTGCGCATCTGGCAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTtgccg                           >  1:5712562/1‑93 (MQ=255)
                                                               tAAGCGGCATAACGCTGCGCATCTGGCAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCcgcg                         >  1:1975174/1‑93 (MQ=255)
                                                                aaGCGGCATAACGCTGCGCATCTGGCCGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGACCGATTTCTCTGGATTCGACGCGa                        >  1:2487777/1‑93 (MQ=255)
                                                                aaGCGGCATAACGCTGCGCATCTGGCAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGa                        >  1:2703981/1‑93 (MQ=255)
                                                                 aGCGGCATAACGCTGCGCATCTGGCAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGAt                       <  2:7690082/93‑1 (MQ=255)
                                                                  gCGGCATAACGCTGCGCATCTGGCAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATc                      <  2:1738913/93‑1 (MQ=255)
                                                                  gCGGCATAACGCTGCGCATCTGGCAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATc                      <  1:5973721/93‑1 (MQ=255)
                                                                    ggCATAACGCTGCGCATCTGGCAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTg                    <  2:5347657/93‑1 (MQ=255)
                                                                    ggCATAACGCTGCGCATCTGGCAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTg                    >  2:4166879/1‑93 (MQ=255)
                                                                       aTAACGCTGCGCATCTGGCAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTgcgc                 >  2:6432999/1‑93 (MQ=255)
                                                                        tAACGCTGCGCATCTGGCAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGcc                >  1:873430/1‑93 (MQ=255)
                                                                         aaCGCTGCGCATCTGGCAGATACGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCa               >  1:6457699/1‑93 (MQ=255)
                                                                          aCGCTGCGCATCTGGCAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAg              >  1:4271184/1‑93 (MQ=255)
                                                                            gCTGCGCATCTGGCAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGcc            <  1:3131923/93‑1 (MQ=255)
                                                                              tGCGCATCTGGCAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCtt          <  1:7448872/93‑1 (MQ=255)
                                                                              tGCGCATCTGGCAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCtt          <  1:2849980/93‑1 (MQ=255)
                                                                              tGCGCATCTGGCAGATGCGACTGTTCTAACGGGAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCACAGCGATCTGCGCCAGCCtt          >  2:2185567/1‑93 (MQ=255)
                                                                               gcgcATCTGGCAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTtg         <  2:4896486/93‑1 (MQ=255)
                                                                                cgcATCTGGCAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTtgc        <  2:923206/93‑1 (MQ=255)
                                                                                 gcATCTGGCAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTtgct       <  1:2212126/93‑1 (MQ=255)
                                                                                   aTCTGGCAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTtgctgc     <  2:4610502/93‑1 (MQ=255)
                                                                                      tGGCAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGcc  >  2:1299977/1‑93 (MQ=255)
                                                                                         |                                                                                         
GTTTACGCCATTAATGGCAGAAGTTGCTGATAGAGGCGACGGAACGTTTCTCGTCGTGGCTGATAAGCGGCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCC  >  NC_000913/3725544‑3725722

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
TAACGTTTACGCCATTAATGGCAGAAGTTGCTGATAGAGGCGACGGAACGTTTCTCGTCGTGGCTGATAAGCGGCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGT  >  NC_000913/3725540‑3725726
                                                                                             |                                                                                             
TAACGTTTACGCCATTAATGGCAGAAGTTGCTGATAGAGGCGACGGAACGTTTCTCGTCGTGGCTGATAAGCGGCATAACGCTGCGCATCTGGC                                                                                               >  SRR4302543.401931/1‑94 (MQ=60)
TAACGTTTACGCCATTAATGGCAGAAGTTGCTGATAGAGGCGACGGAACGTTTCTCGTCGTGGCTGATAAGACGCATAACGCTGCCCATCTGGC                                                                                               >  SRR4302543.401932/1‑94 (MQ=60)
 AACGTTTACGCCATTAATGGCAGAAGTTGCTGATAGAGGCGACGGAACGTTTCTCGTCGTGGCTGATAAGCGGCATAACGCTGCGCATCTGGCA                                                                                              <  SRR4302543.5450940/94‑1 (MQ=60)
 AACGTTTACGCCATTAATGGCAGAAGTTGCTGATAGAGGCGACGGAACGTTTCTCGTCGTGGCTGATAAGCGGCATAACGCTGCGCATCTGGCA                                                                                              >  SRR4302543.455960/1‑94 (MQ=60)
  ACGTTTACGCCATTAATGGCAGAAGTTGCTGATAGAGGCGACGGAACGTTTCTCGTCGTGGCTGATAAGCGGCATAACGCTGCGCATCTGGCAG                                                                                             <  SRR4302543.5118485/94‑1 (MQ=60)
   CGTTTACGCCATTAATGGCAGAAGTTGCTGATAGAGGCGACGGAACGTTTCTCGTCGTGGCTGATAAGCGGCATAACGCTGCGCATCTGGCAGA                                                                                            >  SRR4302543.6114550/1‑94 (MQ=60)
   CGTTTACGCCATTAATGGCAGAAGTTGCTGATAGAGGCGACGGAACGTTTCTCGTCGTGGCTGATAAGCGGCATAACGCTGCGCATCTGGCAGA                                                                                            >  SRR4302543.6630867/1‑94 (MQ=60)
   CGTTTACGCCATTAATGGCAGAAGTTGCTGATAGAGGCGACGGAACGTTTCTCGTCGTGGCTGATAAGCGGCATAACGCTGCGCATCTGGCAGA                                                                                            >  SRR4302543.1568954/1‑94 (MQ=60)
    GTTTACGCCATTAATGGCAGAAGTTGCTGATAGAGGCGACGGAACGTTTCTCGTCGTGGCTGATAAGCGGCATAACGCTGCGCATCTGGCAGAT                                                                                           >  SRR4302543.6836049/1‑94 (MQ=60)
    GTTTACGCCATTAATGGCAGAAGTTGCTGATAGAGGCGACGGAACGTTTCTCGTCGTGGCTGATAAGCGGCATAACGCTGCGCATCTGGCAGAT                                                                                           >  SRR4302543.2413116/1‑94 (MQ=60)
     TTTACGCCATTAATGGCAGAAGTTGCTGATAGAGGCGACGGAACGTTTCTCGTCGTGGCTGATAAGCGGCATAACGCTGCGCATCTGGCAGATG                                                                                          >  SRR4302543.6274507/1‑94 (MQ=60)
       TACGCCATTAATGGCAGAAGTTGCTGATAGAGGCGACGGAACGTTTCTCGTCGTGGCTGATAAGCGGCATAACGCTGCGCATCTGGCAGATGCG                                                                                        <  SRR4302543.3468610/94‑1 (MQ=60)
        ACGCCATTAATGGCAGAAGTTGCTGATAGAGGCGACGGAACGTTTCTCGTCGTGGCTGATAAGCGGCATAACGCTGCGCATCTGGCAGATGCGC                                                                                       >  SRR4302543.5305893/1‑94 (MQ=60)
        ACGCCATTAATGGCAGAAGTTGCTGATAGAGGCGACGGAACGTTTCTCGTCGTGGCTGATAAGCGGCATAACGCTGCGCATCTGGCAGATGCGA                                                                                       >  SRR4302543.4757214/1‑94 (MQ=60)
          GCCATTAATGGCAGAAGTTGCTGATAGAGGCGACGGAACGCTTCTCGTCGTGGCTGATAAGCGGCATAACGCTGCGCATCTGGCAGATGCGACT                                                                                     >  SRR4302543.7574285/1‑94 (MQ=60)
           CCATTAATGGCAGAAGTTGCTGATAGAGGCGACGGAACGTTTCTCGTCGTGGCTGATAAGCGGCATAACGCTGCGCATCTGGCAGATGCGACTG                                                                                    >  SRR4302543.7740179/1‑94 (MQ=60)
            CATTAATGGCAGAAGTTGCTGATAGAGGCGACGGAACGTTTCTCGTCGTGGCTGATAAGCGGCATAACGCTGCGCATCTGGCAGATGCGACTGT                                                                                   >  SRR4302543.6829987/1‑94 (MQ=60)
            CATTAATGGCAGAAGTTGCTGATAGAGGCGACGGAACGTTTCTCGTCGTGGCTGATAAGCGGCATAACGCTGCGCATCTGGCAGATGCGACTGT                                                                                   >  SRR4302543.5409666/1‑94 (MQ=60)
            CATTAATGGCAGAAGTTGCTGATAGAGGCGACGGAACGTTTCTCGTCGTGGCTGATAAGCGGCATAACGCTGCGCATCTGGCAGATGCGACTGT                                                                                   >  SRR4302543.7106147/1‑94 (MQ=60)
            CATTAATGGCAGAAGTTGCTGATAGAGGCGACGGAACGTTTCTCGTCGTGGCTGATAAGCGGCATAACGCTGCGCATCTGGCAGATGCGACTGT                                                                                   >  SRR4302543.2202515/1‑94 (MQ=60)
            CATTAATGGCAGAAGTTGCTGATAGAGGCGACGGAACGTTTCTCGTCGTGGCTGATAAGCGGCATAACGCTGCGCATCTGGCAGATGCGACTGT                                                                                   >  SRR4302543.3033764/1‑94 (MQ=60)
             ATTAATGGCAGAAGTTGCTGATAGAGGCGACGGAACGTTTCTCGTCGTGGCTGATAAGCGGCATAACGCTGCGCATCTGGCAGATGCGACTGTT                                                                                  >  SRR4302543.5741678/1‑94 (MQ=60)
             ATTAATGGCAGAAGTTGCTGATAGAGGCGACGGAACGTTTCTCGTCGTGGCTGATAAGCGGCATAACGCTGCGCATCTGGCAGATGCGACTGTT                                                                                  >  SRR4302543.4181199/1‑94 (MQ=60)
               TAATGGCAGAAGTTGCTGATAGAGGCGACGGAACGTTTCTCGTCGTGGCTGATAAGCGGCATAACGCTGCGCATCTGGCAGATGCGACTGTTCT                                                                                >  SRR4302543.682824/1‑94 (MQ=60)
                AATGGCAGAAGTTGCTGATAGAGGCGACGGAACGTTTCTCGTCGTGGCTGATAAGCGGCATAACGCTGCGCATCTGGCAGATGCGACTGTTCTA                                                                               >  SRR4302543.2410546/1‑94 (MQ=60)
                 ATGGCAGAAGTTGCTGATAGAGGCGACGGAACGTTTCTCGTCGTGGCTGATAAGCGGCATAACGCTGCGCATCTGGCAGATGCGACTGTTCTAA                                                                              >  SRR4302543.7371402/1‑94 (MQ=60)
                 ATGGCAGAAGTTGCTGATAGAGGCGACGGAACGTTTCTCGTCGTGGCTGATAAGCGGCATAACGCTGCGCATCTGGCAGATGCGACTGTTCTAA                                                                              >  SRR4302543.3238784/1‑94 (MQ=60)
                  TGGCAGAAGTTGCTGATAGAGGCGACGGAACGTTTCTCGTCGTGGCTGATAAGCGGCATAACGCTGCGCATCTGGCAGATGCGACTGTTCTAAC                                                                             >  SRR4302543.5819910/1‑94 (MQ=60)
                   GGCAGAAGTTGCTGATAGAGGCGACGGAACGTTTCTCGTCGTGGCTGATAAGCGGCATAACGCTGCGCATCTGGCAGATGCGACTGTTCTAACG                                                                            <  SRR4302543.6946899/94‑1 (MQ=60)
                    GCAGAAGTTGCTGATAGAGGCGACGGAACGTTTCTCGTCGTGGCTGATAAGCGGCATAACGCTGCGCATCTGGCAGATGCGACTGTTCTAACGG                                                                           >  SRR4302543.3891646/1‑94 (MQ=60)
                     CAGAAGTTGCTGATAGAGGCGACGGAACGTTTCTCGTCGTGGCTGATAAGCGGCATAACGCTGCGCATCTGGCAGATGCGACTGTTCTAACGGT                                                                          >  SRR4302543.1765618/1‑94 (MQ=60)
                      AGAAGTTGCTGATAGAGGCGACGGAACGTTTCTCGTCGTGGCTGATAAGCGGCATAACGCTGCGCATCTGGCAGATGCGACTGTTCTAACGGTA                                                                         <  SRR4302543.575235/94‑1 (MQ=60)
                       GAAGTTGCTGATAGAGGCGACGGAACGTTTCTCGTCGTGGCTGATAAGCGGCATAACGCTGCGCATCTGGCAGATGCGACTGTTCTAACGGTAG                                                                        <  SRR4302543.2383340/94‑1 (MQ=60)
                        AAGTTGCTGATAGAGGCGACGGAACGTTTCTCGTCGTGGCTGATAAGCGGCATAACGCTGCGCATCTGGCAGATGCGACTGTTCTAACGGTAGT                                                                       >  SRR4302543.3446395/1‑94 (MQ=60)
                         AGTTGCTGATAGAGGCGACGGAACGTTTCTCGTCGTGGCTGATAAGCGGCATAACGCTGCGCATCTGGCAGATGCGACTGTTCTAACGGTAGTT                                                                      >  SRR4302543.5071700/1‑94 (MQ=60)
                         AGTTGCTGATAGAGGCGACGGAACGTTTCTCGTCGTGGCTGATAAGCGGCATAACGCTGCGCATCTGGCAGATGCGACTGTTCTAACGGTAGTT                                                                      <  SRR4302543.399364/94‑1 (MQ=60)
                          GTTGCTGATAGAGGCGACGGAACGTTTCTCGTCGTGGCTGATAAGCGGCATAACGCTGCGCATCTGGCAGATGCGACTGTTCTAACGGTAGTTG                                                                     >  SRR4302543.1630712/1‑94 (MQ=60)
                          GTTGCTGATAGAGGCGACGGAACGTTTCTCGTCGTGGCTGATAAGCGGCATAACGCTGCGCATCTGGCAGATGCGACTGTTCTAACGGTAGTTG                                                                     >  SRR4302543.6188062/1‑94 (MQ=60)
                            TGCTGATAGAGGCGACGGAACGTTTCTCGTCGTGGCTGATAAGCGGCATAACGCTGCGCATCTGGCAGATGCGACTGTTCTAACGGTAGTTGCG                                                                   >  SRR4302543.357484/1‑94 (MQ=60)
                            TGCTGATAGAGGCGACGGAACGTTTCTCGTCGTGGCTGATAAGCGGCATAACGCTGCGCATCTGGCAGATGCGACTGTTCTAACGGTAGTTGCG                                                                   <  SRR4302543.7243772/94‑1 (MQ=60)
                             GCTGATAGAGGCGACGGAACGTTTCTCGTCGGGGCTGATAAGCGGCATAACGCTGCGCAACTGGCAGATGCGACTGTTCTAACTGTAGTTGCGG                                                                  >  SRR4302543.7703864/1‑94 (MQ=60)
                              CTGATAGAGGCGACGGAACGTTTCTCGTCATGGCTGATAAGCGGCATAACGCTGCGCATCTGGCAGATGCGACTGTTCTAAAGGTAGATGCGGC                                                                 >  SRR4302543.3237587/1‑94 (MQ=60)
                               TGATAGAGGCGACGGAACGTTTCTCGTCGTGGCTGATAAGCGGCATAACGCTGCGCATCTGGCAGATGCGACTGTTCTAACGGTAGTTGCGGCA                                                                >  SRR4302543.5405149/1‑94 (MQ=60)
                                 ATAGAGGCGACGGAACGTTTCTCGTCGTGGCTGATAAGCGGCATAACGCTGCGCATCTGGCAGATGCGACTGTTCTAACGGTAGTTGCGGCAAC                                                              <  SRR4302543.6463664/94‑1 (MQ=60)
                                 ATAGAGGCGACGGAACGTTTCTCGTCGTGGCTGATAAGCGGCATAACGCTGCGCATCTGGCAGATGCGACTGTTCTAACGGTAGTTGCGGCAAC                                                              >  SRR4302543.1591525/1‑94 (MQ=60)
                                  TAGAGGCGACGGAACGTTTCTCGTCGTGGCTGATAAGCGGCATAACGCTGCGCATCTGGCAGATGCGACTGTTCTAACGGTAGTTGCGGCAACA                                                             <  SRR4302543.3070521/94‑1 (MQ=60)
                                  TAGAGGCGACGGAACGTTTCTCGTCGTGGCTGATAAGCGGCATAACGCTGCGCATCTGGCAGATGCGACTGTTCTAACGGTAGTTGCGGCAACA                                                             >  SRR4302543.5669961/1‑94 (MQ=60)
                                   AGAGGCGACGGAACGTTTCTCGTCGTGGCTGATAAGCGGCATAACGCTGCGCATCTGGCAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAA                                                            >  SRR4302543.6506481/1‑94 (MQ=60)
                                   AGAGGCGACGGAACGTTTCTCGTCGTGGCTGATAAGCGGCATAACGCTGCGCATCTGGCAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAA                                                            >  SRR4302543.159646/1‑94 (MQ=60)
                                   AGAGGCGACGGAACGTTTCTCGTCGTGGCTGATAAGCGGCATAACGCTGCGCATCTGGCAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAA                                                            >  SRR4302543.7091164/1‑94 (MQ=60)
                                   AGAGGCGACGGAACGTTTCTCGTCGTGGCTGATAAGCGGCATAACGCTGCGCATCTGGCAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAA                                                            <  SRR4302543.6404265/94‑1 (MQ=60)
                                   AGAGGCGACGGAACGTTTCTCGTCGTGGCTGATAAGCGGCATAACGCTGCGCATCTGGCAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAA                                                            >  SRR4302543.6068247/1‑94 (MQ=60)
                                    GAGGCGACGGAACGTTTCTCGTCGTGGCTGATAAGCGGCATAACGCTGCGCATCTGGCAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAAT                                                           <  SRR4302543.6628519/94‑1 (MQ=60)
                                     AGGCGACGGAACGTTTCTCGTCGTGGCTGATAAGCGGCATAACGCTGCGCATCTGGCAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATT                                                          >  SRR4302543.1367698/1‑94 (MQ=60)
                                        CGACGGAACGTTTCTCGTCGTGGCTGATAAGCGGCATAACGCTGCGCATCTGGCAGATGCGACTGTTCTAACGGTAGGTGCGGCAACAATTCAA                                                       >  SRR4302543.5422912/1‑94 (MQ=60)
                                         GACGGAACGTTTCTCGTCGTGGCTGATAAGCGGCATAACGCTGCGCATCTGGCAGATGCGACTGTTCTAACGGTAGTTGCGGCAACACTTCAAT                                                      >  SRR4302543.6676611/1‑94 (MQ=60)
                                         GACGGAACGTTTCTCGTCGTGGCTGATAAGCGGCATAACGCTGCGCATCTGGCAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAAT                                                      >  SRR4302543.6676610/1‑94 (MQ=60)
                                         GACGGAACGTTTCTCGTCGTGGCTGATAAGCGGCATAACGCTGCGCATCTGGCAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAAT                                                      >  SRR4302543.2859936/1‑94 (MQ=60)
                                          GCGGAACGTTTCTCGTCGTGGCTGATAAGCGGCATAACGCTGCGCATCTGGCAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATG                                                     <  SRR4302543.6829402/94‑1 (MQ=60)
                                          ACGGAACGTTTCTCGTCGTGGCTGATAAGCGGCATAACGCTGCGCATCTGGCAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATG                                                     <  SRR4302543.1041139/94‑1 (MQ=60)
                                           CGGAACGTTTCTCGTCGTGGCTGATAAGCGGCATAACGCTGCGCATCTGGCAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGA                                                    >  SRR4302543.3385864/1‑94 (MQ=60)
                                           CGGAACGTTTCTCGTCGTGGCTGATAAGCGGCATAACGCTGCGCATCTGGCAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGA                                                    >  SRR4302543.7615948/1‑94 (MQ=60)
                                           CGGAACGTTTCTCGTCGTGGCTGATAAGCGGCATAACGCTGCGCATCTGGCAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGA                                                    >  SRR4302543.7497152/1‑94 (MQ=60)
                                           CGGAACGTTTCTCGTCGTGGCTGATAAGCGGCATAACGCTGCGCATCTGGCAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGA                                                    >  SRR4302543.5031764/1‑94 (MQ=60)
                                           CGGAACGTTTCTCGTCGTGGCTGATAAGCGGCATAACGCTGCGCATCTGGCAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGA                                                    >  SRR4302543.2821318/1‑94 (MQ=60)
                                            GGAACGTTTCTCGTCGTGGCTGATAAGCGGCATAACGCTGCGCATCTGGCAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAG                                                   >  SRR4302543.5774444/1‑94 (MQ=60)
                                            GGAACGTTTCTCGTCGTGGCTGATAAGCGGCATAACGCTGCGCATCTGGCAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAG                                                   >  SRR4302543.5774439/1‑94 (MQ=60)
                                            GGAACGTTTCTCGTCGTGGCTGATAAGCGGCATAACGCTGCGCATCTGGCAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAG                                                   >  SRR4302543.5589927/1‑94 (MQ=60)
                                            GGAACGTTTCTCGTCGTGGCTGATAAGCGGCATAACGCTGCGCATCTGGCAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAG                                                   >  SRR4302543.6776174/1‑94 (MQ=60)
                                            GGAACGTTTCTCGTCGTGGCTGATAAGCGGAATAACGCTGCGCATCTGGCAGAGGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAG                                                   >  SRR4302543.1137258/1‑94 (MQ=60)
                                              AACGTTTCTCGTCGTGGCTGATAAGCGGCATAACGCTGCGCATCTGGCAGATGCGACTGTTCTAACGGTAGTTGCTGCAACAATTCAACGAGCG                                                 >  SRR4302543.498709/1‑94 (MQ=60)
                                               ACGTTTCTCGTCGTGGCTGATAAGCGGCATAACGCTGCGCATCTGGCAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGA                                                >  SRR4302543.5424511/1‑94 (MQ=60)
                                               ACGTTTCTCGTCGTGGCTGATAAGCGGCATAACGCTGCGCATCTGGCAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGA                                                >  SRR4302543.2254009/1‑94 (MQ=60)
                                                CGTTTCTCGTCGTGGCTGATAAGCGGCATAACGCTGCGCATCTGGCAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGAT                                               >  SRR4302543.5092071/1‑94 (MQ=60)
                                                 GTTTCTCGTCGTGGCTGATAAGCGGCATAACGCTGCGCATCTGGCAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATT                                              >  SRR4302543.3144378/1‑94 (MQ=60)
                                                 GTTTCTCGTCGTGGCTGATAAGCGGCATAACGCTGCGCATCTGGCAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATT                                              >  SRR4302543.3144377/1‑94 (MQ=60)
                                                  TTTCTCGTCGTGGCTGATAAGCGGCATAACGCTGCGCATCTGGCAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTT                                             <  SRR4302543.6107427/94‑1 (MQ=60)
                                                   TTCTCGTCGTGGCTGATAAGCGGCATAACGCTGCGCATCTGGCAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTC                                            >  SRR4302543.6561969/1‑94 (MQ=60)
                                                   TTCTCGTCGTGGCTGATAAGCGGCATAACGCTGCGCATCTGGCAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTC                                            >  SRR4302543.2476289/1‑94 (MQ=60)
                                                     CTCGTCGTGGCTGATAAGCGGCATAACGCTGCGCATCTGGCAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTC                                          >  SRR4302543.245420/1‑94 (MQ=60)
                                                     CTCGTCGTGGCTGATAAGCGGCATAACGCTGCGCATCTGGCAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTC                                          >  SRR4302543.2190176/1‑94 (MQ=60)
                                                       CGTCGTGGCTGATAAGCGGCATAACGCTGCGCATCTGGCAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTG                                        <  SRR4302543.4161236/94‑1 (MQ=60)
                                                       CGTCGTGGCTGATAAGCGGCATAACGCTGCGCATCTGGCAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTG                                        >  SRR4302543.3974197/1‑94 (MQ=60)
                                                         TCGTGGCTGATAAGCGGCATAACGCTGCGCATCTGGCAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGA                                      >  SRR4302543.4604021/1‑94 (MQ=60)
                                                          CGTGGCTGATAAGCGGCATAACGCTGCGCATCTGGCAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGAT                                     <  SRR4302543.3130037/94‑1 (MQ=60)
                                                          CGTGGCTGATAAGCGGCATAACGCTGCGCATCTGGCAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGAT                                     >  SRR4302543.6705314/1‑94 (MQ=60)
                                                          CGTGGCTGATAAGCGGCATAACGCTGCGCATCTGGCAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGAT                                     >  SRR4302543.4667639/1‑94 (MQ=60)
                                                          CGTGGCTGATAAGCGGCATAACGCTGCGCATCTGGCAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCACTGGAT                                     <  SRR4302543.159103/94‑1 (MQ=60)
                                                          CGTGGCTGATAAGCGGCATAACGCTGCGCATCTGGCAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATCAGCGAATTTTCTGGAT                                     >  SRR4302543.1023079/1‑94 (MQ=60)
                                                           GTGGCTGATAAGCGGCATAACGCTGCGCATCTGGCAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATT                                    <  SRR4302543.5595016/94‑1 (MQ=60)
                                                           GTGGCTGATAAGCGGCATAACGCTGCGCATCTGGCAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATT                                    <  SRR4302543.3962841/94‑1 (MQ=60)
                                                            TGGCTGATAAGCGGCATAACGCTGCGCATCTGGCAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTC                                   >  SRR4302543.5662256/1‑94 (MQ=60)
                                                            TGGCTGATAAGCGGCATAACGCTGCGCAACTCGCAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCaaattctctacattc                                   >  SRR4302543.7448189/1‑79 (MQ=60)
                                                             GGCTGATAAGCGGCATAACGCTGCGCATCTGGCAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCGATGAGCGCTTTCTCTGGATTCG                                  >  SRR4302543.7139192/1‑94 (MQ=60)
                                                             GGCTGATAAGCGGCATAACGCTGCGCATCTGGCAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCG                                  <  SRR4302543.4528364/94‑1 (MQ=60)
                                                             GGCTGATAAGCGGCATAACGCTGCGCATCTGGCAGATGCGACTGTTCTAACGGTAGTCACGGCAACCATTCAATAAGCGATTTCTCTGGATTac                                  >  SRR4302543.2665912/1‑92 (MQ=60)
                                                               CTGATAAGCGGCATAACGCTGCGCATCTGGCAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTAGCC                                >  SRR4302543.4718776/1‑94 (MQ=60)
                                                                TGATAAGCGGCATAACGCTGCGCATCTGGCAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTTGCCG                               >  SRR4302543.5764681/1‑94 (MQ=60)
                                                                  ATAAGCGGCATAACGCTGCGCATCTGGCAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCG                             >  SRR4302543.7335961/1‑94 (MQ=60)
                                                                  ATAAGCGGCATAACGCTGCGCATCTGGCAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCG                             >  SRR4302543.4251924/1‑94 (MQ=60)
                                                                  ATAAGCGGCATAACGCTGCGCATCTGGCAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCG                             >  SRR4302543.524005/1‑94 (MQ=60)
                                                                  ATAAGCGGCATAACGCTGCGCATCTGGCAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCG                             >  SRR4302543.1992884/1‑94 (MQ=60)
                                                                  ATAAGCGGCATAACGCTGCGCATCTGGCAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCG                             >  SRR4302543.4552861/1‑94 (MQ=60)
                                                                  ATAAGCGGCATAACGCTGCGCATCTGGCAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCG                             >  SRR4302543.4394341/1‑94 (MQ=60)
                                                                   TAAGCGGCATAACGCTGCGCATCTGGCCGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGACCGATTTCTCTGGATTCGACGCGA                            >  SRR4302543.2510319/1‑94 (MQ=60)
                                                                   TAAGCGGCATAACGCTGCGCATCTGGCAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGA                            >  SRR4302543.2728514/1‑94 (MQ=60)
                                                                    AAGCGGCATAACGCTGCGCATCTGGCAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGAT                           >  SRR4302543.870029/1‑94 (MQ=60)
                                                                    AAGCGGCATAACGCTGCGCATCTGGCAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGAT                           >  SRR4302543.552012/1‑94 (MQ=60)
                                                                      GCGGCATAACGCTGCGCATCTGGCAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCT                         <  SRR4302543.1255804/94‑1 (MQ=60)
                                                                      GCGGCATAACGCTGCGCATCTGGCAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCT                         <  SRR4302543.6028438/94‑1 (MQ=60)
                                                                      GCGGCATAACGCTGCGCATCTGGCAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCT                         >  SRR4302543.2552930/1‑94 (MQ=60)
                                                                      ACGGCAGAACGCTGCGCATCTGGGAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCT                         <  SRR4302543.6028439/94‑1 (MQ=60)
                                                                        GGCATAACGCTGCGCATCTGGCAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGC                       >  SRR4302543.3000371/1‑94 (MQ=60)
                                                                          CATAACGCTGCGCATCTGGCAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGC                     >  SRR4302543.3992628/1‑94 (MQ=60)
                                                                          CATAACGCTGCGCATCTGGCAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGC                     >  SRR4302543.7205305/1‑94 (MQ=60)
                                                                          CATAACGCTGCGCATCTGGCAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGC                     <  SRR4302543.1214398/94‑1 (MQ=60)
                                                                           ATAACGCTGCGCATCTGGCAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGagca                    >  SRR4302543.489774/1‑90 (MQ=60)
                                                                           ATAACGCTGCGCATCTGGCAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCC                    >  SRR4302543.881210/1‑94 (MQ=60)
                                                                            TAACGCTGCGCATCTGGCAGATACGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCA                   >  SRR4302543.6518548/1‑94 (MQ=60)
                                                                             AACGCTGCGCATCTGGCAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAG                  >  SRR4302543.3833779/1‑94 (MQ=60)
                                                                             AACGCTGCGCATCTGGCAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAG                  >  SRR4302543.4309999/1‑94 (MQ=60)
                                                                               CGCTGCGCATCTGGCAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCC                >  SRR4302543.1658112/1‑94 (MQ=60)
                                                                               CGCTGCGCATCTGGCAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCC                <  SRR4302543.1674451/94‑1 (MQ=60)
                                                                               CGCTGCGCATCTGGCAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCC                >  SRR4302543.7091920/1‑94 (MQ=60)
                                                                                GCTGCTCATCTGGTAGATGAGACTGTTCTAACGGTGGTTGCGGCAACAATTCCATGGGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCT               <  SRR4302543.3112056/94‑1 (MQ=60)
                                                                                GCTGCGCATCTGGCAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCT               >  SRR4302543.7317281/1‑94 (MQ=60)
                                                                                GCTGCGCATCTGGCAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCT               <  SRR4302543.3112057/94‑1 (MQ=60)
                                                                                GCTGCGCATCTGGCAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCT               <  SRR4302543.3160092/94‑1 (MQ=60)
                                                                                 CTGCGCATCTGGCAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTT              >  SRR4302543.3333106/1‑94 (MQ=60)
                                                                                  TGCGCATCTGGCAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTG             <  SRR4302543.7520256/94‑1 (MQ=60)
                                                                                  TGCGCATCTGGCAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTG             <  SRR4302543.2875997/94‑1 (MQ=60)
                                                                                  TGCGCATCTGGCAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTG             >  SRR4302543.6873368/1‑94 (MQ=60)
                                                                                   GCGCATCTGGCAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGC            <  SRR4302543.2574758/94‑1 (MQ=60)
                                                                                     GCATCTGGCAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTG          <  SRR4302543.2232052/94‑1 (MQ=60)
                                                                                      CATCTGGCAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCTCCGCGATCTCCGCCAacatgcctcg         >  SRR4302543.2038303/1‑84 (MQ=60)
                                                                                      CATCTGGCAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGC         <  SRR4302543.2711703/94‑1 (MQ=60)
                                                                                       ATCTGGCAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCG        >  SRR4302543.3366132/1‑94 (MQ=60)
                                                                                       ATCTGGCAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCG        >  SRR4302543.4429542/1‑94 (MQ=60)
                                                                                       ATCTGGCAGATGCGACTGTTCTAACGGGAGTTGCAGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCCGCCTTGCTGAG        >  SRR4302543.1791965/1‑94 (MQ=60)
                                                                                         CTGGCAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCC      >  SRR4302543.247806/1‑94 (MQ=60)
                                                                                         CTGGCAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCC      >  SRR4302543.5844393/1‑94 (MQ=60)
                                                                                           GGCAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCA    >  SRR4302543.7887569/1‑94 (MQ=60)
                                                                                           GGCAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCA    >  SRR4302543.5850630/1‑94 (MQ=60)
                                                                                             CAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGT  >  SRR4302543.6784097/1‑94 (MQ=60)
                                                                                             |                                                                                             
TAACGTTTACGCCATTAATGGCAGAAGTTGCTGATAGAGGCGACGGAACGTTTCTCGTCGTGGCTGATAAGCGGCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGT  >  NC_000913/3725540‑3725726

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑