Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 1696 279.3 15874187 97.8% 15524954 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,804,224 A→G T157T (ACT→ACC)  rfaG ← glucosyltransferase I

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,804,2240AG99.7% 903.4 / ‑5.3 290T157T (ACT→ACC) rfaGglucosyltransferase I
Reads supporting (aligned to +/- strand):  ref base A (0/1);  new base G (173/116);  total (173/117)
Fisher's exact test for biased strand distribution p-value = 4.03e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.06e-01

Only 100 of 325 total aligned reads displayed.

GCGATAAATTTCACGGCTGTTTGGGATTTGCTCACTGTATTTTCTGTCCGGATAAATACCGGGAGGAAGAATTTGAAAACGTTCAGGTTCAGTTTGATAATGCTTCTGGAAATCGGCGATTTGCTTATCGGTCAGCATCATAAGTTTCGTCGATTTACCCTGCTCGAAAGTCGCTCGCTC  >  NC_000913/3804134‑3804313
                                                                                          |                                                                                         
  gATAAATTTCACGGCTGTTTGGGATTTGCTCACTGTATTTTCTGTCCGGATAAATACCGGGAGGAAGAATTTGAAAACGTTCAGGTTCGGttt                                                                                       <  1:4636797/93‑1 (MQ=255)
  gATAAATTTCACGGCTGTTTGGGATTTGCTCACTGTATTTTCTGTCCGGATAAATACCGGGAGGAAGAATTTGAAAACGTTCAGGTTCGGttt                                                                                       >  2:4148199/1‑93 (MQ=255)
  gATAAATTTCACGGCTGTTTGGGATTTGCTCACTGTATTTTCTGTCCGGATAAATACCGGGAGGAAGAATTTGAAAACGATCAGGTTCGGttt                                                                                       >  2:4472086/1‑93 (MQ=255)
   aTAAATTTCACGGCTGTTTGGGATTTGCTCACTGTATTTTCTGTCCGGATAAATACCGGGAGGAAGAATTTGAAAACGTTCAGGTTCGGTTTg                                                                                      >  2:1143605/1‑93 (MQ=255)
    tAAATTTCACGGGTGTTTGGGATTTGCTCACTGTATTTTCTGTCCGGATAAATACCGGGAGGAAGAATTTGAAAACGTTCAGGTTCGGTTTGa                                                                                     <  1:6326754/93‑1 (MQ=255)
       aTTTCACGGCTGTTTGGGATTTGCTCACTGTATTTTCTGTCCGGATAAATACCGGGAGGAAGAATTTGAAAACGTTCAGGTTCGGTTTGATaa                                                                                  <  2:7517257/93‑1 (MQ=255)
       aTTTCACGGCTGTTTGGGATTTGCTCACTGTATTTTCTGTCCGGATAAATACCGGGAGGAAGAATTTGAAAACGTTCAGGTTCGGTTTGATaa                                                                                  <  2:6213647/93‑1 (MQ=255)
       aTTTCACGGCTGTTTGGGATTTGCTCACTGTATTTTCTGTCCGGATAAATACCGGGAGGAAGAATTTGAAAACGTTCAGGTTCGGTTTGATaa                                                                                  >  2:3780320/1‑93 (MQ=255)
        tttCACGGCTGTTTGGGATTTGCTCACTGTATTTTCTGTCCGGATAAATACCGGGAGGAAGAATTTGAAAACGTTCAGGTTCGGTTTGATAAt                                                                                 >  2:5879942/1‑93 (MQ=255)
        tttCACGGCTGTTTGGGATTTGCTCACTGTATTTTCTGTCCGGATAAATACAGGGAGGAAGAATTTGAAAACGTTCAGGTTCGGTTTGATAAt                                                                                 <  2:6084022/93‑1 (MQ=255)
          tCACGGCTGTTTGGGATTTGCTCACTGTATTTTCTGTCCGGATAAATACCGGGAGGAAGAATTTGAAAACGTTCAGGTTCGGTTTGATAATGc                                                                               <  1:106833/93‑1 (MQ=255)
            aCGGCTGTTTGGGATTTGCTCACTGTATTTTCTGTCCGGATAAATACCGGGAGGAAGAATTTGAAAACGTTCAGGTTCGGTTTGATAATGCtt                                                                             >  2:5306157/1‑93 (MQ=255)
             cGGCTGTTTGGGATTTGCTCACTGTATTTTCTGTCCGGATAAATACCGGGAGGAAGAATTTGAAAACGTTCAGGTTCGGTTTGATAATGCTTc                                                                            <  1:667999/93‑1 (MQ=255)
             cGGCTGTTTGGGATTTGCTCACTGTATTTTCTGTCCGGATAAATACCGGGAGGAAGAATTTGAAAACGTTCAGGTTCGGTTTGATAATGCTTc                                                                            >  2:5495664/1‑93 (MQ=255)
             cGGCTGTTTGGGATTTGCTCACTGTATTTTCTGTCCGGATAAATACCGGGAGGAAGAATTTGAAAACGTTCAGGTTCGGTTTGATAATGCTTc                                                                            <  1:5263915/93‑1 (MQ=255)
             cGGCTGTTTGGGATTTGCTCACTGTATTTTCTGTCCGGATAAATACCGGGAGGAAGAATTTGAAAACGTTCAGGTTCGGTTTGATAATGCTTc                                                                            <  2:726942/93‑1 (MQ=255)
              ggCTGTTTGGGATTTGCTCACTGTATTTTCTGTCCGGATAAATACCGGGAGGAAGAATTTGAAAACGTTCAGGTTCGGTTTGATAATGCTTCt                                                                           <  2:3684734/93‑1 (MQ=255)
              ggCTGTTTGGGATTTGCTCACTGTATTTTCTGTCCGGATAAATACCGGGAGGAAGAATTTGAAAACGTTCAGGTTCGGTTTGATAATGCTTCt                                                                           <  2:1778671/93‑1 (MQ=255)
                cTGTTTGGGATTTGCTCACTGTATTTTCTGTCCGGATAAATACCGGGAGGAAGAATTTGAAAACGTTCAGGTTCGGTTTGATAATGCTTCTgg                                                                         >  1:1541509/1‑93 (MQ=255)
                 tGTTTGGGATTTGCTCACTGTATTTTCTGTCCGGATAAATACCGGGAGGAAGAATTTGAAAACGTTCAGGTTCGGTTTGATAATGCTTCTGGa                                                                        >  1:5389046/1‑93 (MQ=255)
                  gTTTGGGATTTGCTCACTGTATTTTCTGTCCGGATAAATACCGGGAGGAAGAATTTGAAAACGTTCAGGTTCGGTTTGATACTGCTTCTGGaa                                                                       >  1:4786126/1‑93 (MQ=255)
                  gTTTGGGATTTGCTCACTGTATTTTCTGTCCGGATAAATACCGGGAGGAAGAATTTGAAAACGTTCAGGTTCGGTTTGATAATGCTTCTGGaa                                                                       >  1:474908/1‑93 (MQ=255)
                   tttGGGATTTGCTCACTGTATTTTCTGTCCGGATAAATACCGGGAGGAAGAATTTGAAAACGTTCAGGTTCGGTTTGATAATGCTTCTGGaaa                                                                      >  1:7243322/1‑93 (MQ=255)
                   tttGGGATTTGCTCACTGTATTTTCTGTCCGGATAAATACCGGGAGGAAGAATTTGAAAACGTTCAGGTTCGGTTTGATAATGCTTCTGGaaa                                                                      >  2:3412696/1‑93 (MQ=255)
                     tGGGATTTGCTCACTGTATTTTCTGTCCGGATAAATACCGGGAGGAAGAATTTGAAAACGTTCAGGTTCGGTTTGATAATGCTTCTGGAAATc                                                                    <  2:3027190/93‑1 (MQ=255)
                     tGGGATTTGCTCACTGTATTTTCTGTCCGGATAAATACCGGGAGGAAGAATTTGAAAACGTTCAGGTTCGGTTTGATAATGCTTCTGGAAATc                                                                    <  2:2644595/93‑1 (MQ=255)
                        gATTTGCTCACTGTATTTTCTGTCCGGATAAATACCGGGAGGAAGAATTTGAAAACGTTCAGGTTCGGTTTGATAATGCTTCTGGAAATCGGc                                                                 <  2:7836962/93‑1 (MQ=255)
                         aTTTGCTCACTGTATTTTCTGTCCGGATAAATACCGGGAGGAAGAATTTGAAAACGTTCAGGTTCGGTTTGATAATGCTTCTGGAAATCGGCg                                                                >  2:6317009/1‑93 (MQ=255)
                         aTTTGCTCACTGTATTTTCTGTCCGGATAAATACCGGGAGGAAGAATTTGAAAACGTTCAGGTTCGGTTTGATAATGCTTCTGGAAATCGGCg                                                                >  2:1609739/1‑93 (MQ=255)
                          tttGCTCACTGTATTTTCTGTCCGGATAAATACCGGGAGGAAGAATTTGAAAACGTTCAGGTTCGGTTTGATAATGCTTCTGGAAATCGGCGa                                                               <  2:4671345/93‑1 (MQ=255)
                            tGCTCACTGTATTTTCTGTCCGGATAAATACCGGGAGGAAGAATTTGAAAACGTTCAGGTTCGGTTTGATAATGCTTCTGGAAATCGGCGAtt                                                             <  1:5566896/93‑1 (MQ=255)
                               tCACTGTATTTTCTGTCCGGATAAATACCGGGAGGAAGAATTTGAAAACGTTCAGGTTCGGTTTGATAATGCTTCTGGAAATCGGCGATTTGc                                                          <  2:4997443/93‑1 (MQ=255)
                                cACTGTGTTTTATGTCCAGATAAATAACGGGAGGAAGAATTTGCAAACGTTCAGGTTCGGTTTGATAATGCTTCTGAAAATCGGCGATTTgat                                                         <  2:7046126/93‑3 (MQ=255)
                                cACTGTATTTTCTGTCCGGATAAATACCGGGAGGAAGAATTTGAAAACGTTCAGGTTCGGTTTGATAATGCTTCTGGAAATCGGCGATTTGCt                                                         <  1:3481881/93‑1 (MQ=255)
                                 aCTGTATTTTCTGTCCGGATAAATACCGGGAGGAAGAATTTGAAAACGTTCAGGTTCGGTTTGATAATGCTTCTGGAAATCGGCGATTTGCtt                                                        >  1:6387763/1‑93 (MQ=255)
                                 aCTGTATTTTCTGTCCGGATAAATACCGGGAGGAAGAATTTGAAAACGTTCAGGTTCGGTTTGATAATGCTTCTGGAAATCGGCGATTTGCtt                                                        >  2:2816608/1‑93 (MQ=255)
                                 aCTGTATTTTCTGTACGGATAACTACCGGGACGAAGAATTTGAAACCGTTCAGGTTCGGTTTGATAATGCTTCTGGAAATCGGCGATTTGCtt                                                        <  1:7770362/93‑1 (MQ=255)
                                  cTGTATTTTCTGTCCGGATAAATACCGGGAGGAAGAATTTGAAAACGTTCAGGTTCGGTTTGATAATGCTTCTGGAAATCGGCGATTTGCTTa                                                       >  1:1398943/1‑93 (MQ=255)
                                  cTGTATTTTCTGTCCGGATAAATACCGGGAGGAAGAATTTGAAAACGTTCAGGTTCGGTTTGATAATGCTTCTGGAAATCGGCGATTTGCTTa                                                       >  2:4904351/1‑93 (MQ=255)
                                  cTGTATTTTCTGTCCGGATAAATACCGGGAGGAAGAATTTGAAAACGTTCAGGTTCGGTTTGATAATGCTTCTGGAAATCGGCGATTTGCTTa                                                       <  2:1065235/93‑1 (MQ=255)
                                  cTGTATTTTCTGTCCGGATAAATACCGGGAGGAAGAATTTGAAAACGTTCAGGTTCGGTTTGATAATGCTTCTGGAAATCGGCGATTTGCTTa                                                       <  1:3890976/93‑1 (MQ=255)
                                   tGTATTTTCTGTCCGGATAAATACCGGGAGGAAGAATTTGAAAACGTTCAGGTTCGGTTTGATAATGCTTCTGGAAATCGGCGATTTGCTTag                                                      <  2:5617554/93‑2 (MQ=255)
                                   tGTATTTTCTGTCCGGATAAATACCGGGAGGAAGAATTTGAAAACGTTCAGGTTCGGTTTGATAATGCTTCTGGAAATCGGCGATTTGCTTAt                                                      <  2:6873964/93‑1 (MQ=255)
                                   tGTATTTTCTGTCCGGATAAATACCGGGAGGAAGAATTTGAAAACGTTCAGGTTCGGTTTGATAATGCTTCTGGAAATCGGCGATTTGCTTAt                                                      <  1:2703033/93‑1 (MQ=255)
                                   tGTATTTTCTGTCCGGATAAATACCGGGAGGAAGAATTTGAAAACGTTCAGGTTCGGGTTGATAATGCTTCTGGAAATCGGCGATTTGCTTAt                                                      >  1:3841609/1‑93 (MQ=255)
                                    gTATTTTCTGTCCGGATAAATACCGGGAGGAAGAATTTGAAAACGTTCAGGTTCGGTTTGATAATGCTTCTGGAAATCGGCGATTTGCTTATc                                                     >  2:5753208/1‑93 (MQ=255)
                                     tATTTTCTGTCCGGATAAATACCGGGAGGAAGAATTTGAAAACGTTCAGGTTCGGTTTGATAATGCTTCTGGAAATCGGCGATTTGCTTATCg                                                    >  1:2383220/1‑93 (MQ=255)
                                      aTTTTCTGTCCGGATAAATACCGGGAGGAAGAATTTGAAAACGTTCAGGTTCGGTTTGATAATGCTTCTGGAAATCGGCGATTTGCTTATCgg                                                   <  1:3213063/93‑1 (MQ=255)
                                       ttttCTGTCCGGATAAATACCGGGAGGAAGAATTTGAAAACGTTCAGGTTCGATTTGATAATGCTTCTGGAAATCGGCGATTTGCTTATCGGt                                                  <  2:6647482/93‑1 (MQ=255)
                                          tCTGTCCGGATAAATACCGGGAGGAAGAATTTGAAAACGTTCAGGTTCGGTTTGATAATGCTTCTGGAAATCGGCGATTTTCTTATCGGTCAg                                               >  2:1988543/1‑93 (MQ=255)
                                           cTGTCCGGATAAATACCGGGAGGAAGAATTTGAAAACGTTCAGGTTCGGTTTGATAATGCTTCTGGAAATCGGCGATTTGCTTATCGGTCAGc                                              >  2:6444985/1‑93 (MQ=255)
                                            tGTCCGGATAAATACCGGGAGGAAGAATTTGAAAACGTTCAGGTTCGGTTTGATAATGCTTCTGGAAATCGGCGATTTGCTTATCGGTCAGca                                             >  2:7642341/1‑93 (MQ=255)
                                               ccGGATAAATACCGGGAGGAAGAATTTGAAAACGTTCAGGTTCGGTTTGATAATGCTTCTGGAAATCGGCGATTTGCTTATCGGTCAGcatca                                          <  2:821445/93‑1 (MQ=255)
                                               ccGGATAAATACCGGGAGGAAGAATTTGAAAACGTTCAGGTTCGGTTTGATAATGCTTCTGGAAATCGGCGATTTGCTTATCGGTCAGcatca                                          <  1:6024755/93‑1 (MQ=255)
                                                cGGATAAATACCGGGAGGAAGAATTTGAAAACGTTCAGGTTCGGTTTGATAATGCTTCTGGAAATCGGCGATTTGCTTATCGGTCAGcatcat                                         >  2:127689/1‑93 (MQ=255)
                                                  gATAAATACCGGGAGGAAGAATTTGAAAACGTTCAGGTTCGGTTTGATAATGCTTCTGGAAATCGGCGATTTGCTTATCGGTCAGCATCATaa                                       >  1:5138167/1‑93 (MQ=255)
                                                  gATAAATACCGGGAGGAAGAATTTGAAAACGTTCAGGTTCGGTTTGATAATGCTTCTGGAAATCGGCGATTTGCTTATCGGTCAGCATCATaa                                       >  1:5830147/1‑93 (MQ=255)
                                                  gATAAATACCGGGAGGAAGAATTTGAAAACGTTCAGGTTCGGTTTGATAATGCTTCTGGAAATCGGCGATTTGCTTATCGGTCAGCATCATaa                                       >  1:7596910/1‑93 (MQ=255)
                                                    tAAATACCGGGAGGAAGAATTTGAAAACGTTCAGGTTCGGTTTGATAATGCTTCTGGAAATCGGCGATTTGCTTATCGGTCAGCATCATAAGt                                     >  1:119300/1‑93 (MQ=255)
                                                    tAAATACCGGGAGGAAGAATTTGAAAACGTTCAGGTTCGGTTTGATAATGCTTCTGGAAATCGGCGATTTGCTTATCGGTCAGCATCATAAGt                                     >  1:7361272/1‑93 (MQ=255)
                                                     aaaTACCGGGAGGAAGAATTTGAAAACGTTCAGGTTCGGTTTGATAATGCTTCTGGAAATCGGCGATTTGCTTATCGGTCAGCATCATAAGtt                                    >  2:7079148/1‑93 (MQ=255)
                                                         aCCGGGAGGAAGAATTTGAAAACGTTCAGGTTCGGTTTGATAATGCTTCTGGAAATCGGCGATTTGCTTATCGGTCAGCATCATAAGTTtcgt                                <  2:1464137/93‑1 (MQ=255)
                                                          ccGGGAGGAAGAATTTGAAAACGTTCAGGTTCGGTTTGATAATGCTTCTGGAAATCGGCGATTTGCTTATCGGTCAGCATCATAAGTTtcgtc                               <  2:5346664/93‑1 (MQ=255)
                                                           cGGGAGGAAGAATTTGAAAACGTTCAGGTTCGGTTTGATAATGCTTCTGGAAATCGGCGATTTGCTTATCGGTCAGCATCATAAGTTtcgtcg                              >  2:2553298/1‑93 (MQ=255)
                                                           cGGGAGGAAGAATTTGAAAACGTTCAGGTTCGGTTTGATAATGCTTCTGGAAATCGGCGATTTGCTTATCGGTCAGCATCATAAGTTtcgtcg                              >  1:6631903/1‑93 (MQ=255)
                                                            gggAGGAAGAATTTGAAAACGTTCAGGTTCGGTTTGATAATGCTTCTGGAAATCGGCGATTTGCTTATCGGTCAGCATCATAAGTTTCGTCGa                             >  1:7553801/1‑93 (MQ=255)
                                                             ggaggaATAATTTGAAAACGTTCAGGTTCGGTTTGATAATGCTTCTGGAAATCGGCGATTTGCTTATCGGTCAGCATCATAAGTTTCGTCGAt                            <  1:3677611/93‑1 (MQ=255)
                                                             ggaggaAGAATTTGAAAACGTTCAGGTTCGGTTTGATAATGCTTCTGGAAATCGGCGATTTGCTTATCGGTCAGCATCATAAGTTTCGTCGAt                            <  2:2362586/93‑1 (MQ=255)
                                                              gaggaAGAATTTGAAAACGTTCAGGTTCGGTTTGATAATGCTTCTGGAAATCGGCGATTTGCTTATCGGTCAGCATCATAAGTTTCGTCGAtt                           <  1:5538970/93‑1 (MQ=255)
                                                              gaggaAGAATTTGAAAACGTTCAGGTTCGGTTTGATAATGCTTCTGGAAATCGGCGATTTGCTTATCGGTCAGCATCATAAGTTTCGTCGAtt                           >  1:5004180/1‑93 (MQ=255)
                                                                ggaAGAATTTGAAAACGTTCAGGTTCGGTTTGATAATGCTTCTGGAAATCGGCGATTTGCTTATCGGTCAGCATCATAAGTTTCGTCGATTTa                         >  2:1899448/1‑93 (MQ=255)
                                                                 gaagaaTTTGAAAACGTTCAGGTTCGGTTTGATAATGCTTCTGGAAATCGGCGATTTGCTTATCGGTCAGCATCATAAGTTTCGTCGATTTAc                        >  2:4844253/1‑93 (MQ=255)
                                                                 gaagaaTTTGAAAACGTTCAGGTTCGGTTTGATAATGCTTCTGGAAATCGGCGATTTGCTTATCGGTCAGCATCATAAGTTTCGTCGATTTAc                        >  2:246928/1‑93 (MQ=255)
                                                                 gaagaaTTTGAAAACGTTCAGGTTCGGTTTGATAATGCTTCTGGAAATCGGCGATTTGCTTATCGGTCAGCATCATAAGTTTCGTCGATTTAc                        >  1:4573490/1‑93 (MQ=255)
                                                                   agaaTTTGAGAACGTTCAGGTTCGGTTTGATAATGCTTCTGGAAATCGGCGATTTGCTTATCGGTCAGCATCATAAGTTTCGTCGATTTAccc                      <  1:5913293/93‑1 (MQ=255)
                                                                   agaaTTTGAAAACGTTCAGGTTCGGTTTGATAATGCTTCTGGAAATCGGCGATTTGCTTATCGGTCAGCATCATAAGTTTCGTCGATTTAccc                      <  2:6160891/93‑1 (MQ=255)
                                                                   agaaTTTGAAAACGTTCAGGTTCGGTTTGATAATGCTTCTGGAAATCGGCGATTTGCTTATCGGTCAGCATCATAAGTTTCGTCGATTTAccc                      <  1:4433893/93‑1 (MQ=255)
                                                                   agaaTTTGAAAACGTTCAGGTTCGGTTTGATAATGCTTCTGGAAATCGGCGATTTGCTTATCGGTCAGCATCATAAGTTTCGTCGATTTAccc                      <  2:6731862/93‑1 (MQ=255)
                                                                   agaaTTTGAAAACGTTCAGGTTCGGTTTGATAATGCTTCTGGAAATCGGCGATTTGCTTATCGGTCAGCATCATAAGTTTCGTCGATTTAccc                      >  1:2148097/1‑93 (MQ=255)
                                                                      aTTTGAAAACGTTCAGGTTCGGTTTGATAATGCTTCTGGAAATCGGCGATTTGCTTATCGGTCAGCATCATAAGTTTCGTCGATTTACCCTGc                   >  2:7141039/1‑93 (MQ=255)
                                                                        ttGAAAACGTTCAGGTTCGGTTTGATAATGCTTCTGGAAATCGGCGATTTGCTTATCGGTCAGCATCATAAGTTTCGTCGATTTACCCTGCTc                 >  2:3592737/1‑93 (MQ=255)
                                                                         tGAAAACGTTCAGGTTCGGTTTGATAATGCTTCTGGAAATCGGCGATTTGCTTATCGGTCAGCATCATAAGTTTCGTCGATTTACCCTGCTCg                >  1:1871546/1‑93 (MQ=255)
                                                                          gAAAACGTTCAGGTTCGGTTTGATAATGCTTCTGGAAATCGGCGATTTGCTTATCGGTCAGCATCATAAGTTTCGTCGATTTACCCTGCTCGa               >  2:3194367/1‑93 (MQ=255)
                                                                            aaaCGTTCAGGTTCGGTTTGATAATGCTTCTGGCAATCGGCGATTTGCTTATCGGTCAGCATCATAAGTTTCGTCGATTTACCCTGCTCGaaa             >  1:3991459/1‑93 (MQ=255)
                                                                            aaaCGTTCAGGTTCGGTTTGATAATGCTTCTGGAAATCGGCGATTTGCTTATCGGTCAGCATCATAAGTTTCGTCGATTTACCCTGCTCGaaa             >  1:6903820/1‑93 (MQ=255)
                                                                            aaaCGTTCAGGTTCGGTTTGATAATGCTTCTGGAAATCGGCGATTTGCTTATCGGTCAGCATCATAAGTTTCGTCGATTTACCCTGCTCGaaa             <  2:3293672/93‑1 (MQ=255)
                                                                            aaaCGTTCAGGTTCGGTTTGATAATGCTTCTGGAAATCGGCGATTTGCTTATCGGTCAGCATCATAAGTTTCATCGATTTACCCTGCTCGaaa             <  2:1571815/93‑1 (MQ=255)
                                                                            aaaCGTTCAGGTTCGGTTTGATAATGCTTCTGGAAATCGGCGATTTGCTTATCGGTCAGCATATTAAGTTTCGTCGATTTACCCTGCTCGaaa             >  1:6730495/1‑93 (MQ=255)
                                                                             aaCGTTCAGGTTCGGTTTGATAATGCTTCTGGAAATCGGCGATTTGCTTATCGGTCAGCATCATAAGTTTCGTCGATTTACCCTGCTCGAAAg            <  2:6234414/93‑1 (MQ=255)
                                                                             aaCGTTCAGGTTCGGTTTGATAATGCTTCTGGAAATCGGCGATTTGCTTATCGGTCAGCATCATAAGTTTCGTCGATTTACCCTGCTCGAAAg            >  1:3607789/1‑93 (MQ=255)
                                                                              aCGTTCAGGTTCGGTTTGATAATGCTTCTGGAAATCGGCGATTTGCTTATCGGTCAGCATCATAAGTTTCGTCGATTTACCCTGCTCGAAAGt           >  1:801904/1‑93 (MQ=255)
                                                                               cGTTCAGGTTCGGTTTGATAATGCTTCTGGAAATCGGCGATTTGCTTATCGGTCAGCATCATAAGTTTCGTCGATTTACCCTGCTCGAAAGtc          <  1:1934650/93‑1 (MQ=255)
                                                                               cGTTCAGGTTCGGTTTGATAATGCTTCTGGAAATCGGCGATTTGCTTATCGGTCAGCATCATAAGTTTCGTCGATTTACCCTGCTCGAAAGtc          >  2:3845201/1‑93 (MQ=255)
                                                                                gttcaggttcggTTTTATAATGCTTCTGGAAATCGGCGATTTGCTTATCGGTCAGCATCATAAGTTTCGTCGATTTACCCTGCTCGAAAGtcg         >  2:7230551/1‑93 (MQ=255)
                                                                                gttcaggttcggTTTGATAATGCTTCTGGAAATCGGCGATTTGCTTATCGGTCAGCATCATAAGTTTCGTCGATTTACCCTGCTCGAAAGtcg         >  1:773923/1‑93 (MQ=255)
                                                                                   caggttcggTTTGATAATGCTTCTGGAAATCGGCGATTTGCTTATCGGTCAGCATCATAAGTTTCGTCGATTTACCCTGCTCGAAAGtcgctc      >  2:2148139/1‑93 (MQ=255)
                                                                                   caggttcggTTTGATAATGCTTCTGGAAATCGGCGATTTGCTTATCGGTCAGCATCATAAGTTTCGTCGATTTACCCTGCTCGAAAGtcgctc      <  2:594517/93‑1 (MQ=255)
                                                                                   caggttcggTTTGATAATGCTTCTGGAAATCGGCGATTTGCTTATCGGTCAGCATCATAAGTTTCGTCGATTTACCCTGCTCGAAAGtcgctc      >  1:6557639/1‑93 (MQ=255)
                                                                                   caggttcggTTTGATAATGCTTCTGGAAATCGGCGATTTGCTTATCGGTCAGCATCATAAGTTTCGTCGAGTTACTCTGCTCGAAAGtcgctc      >  1:7044831/1‑93 (MQ=255)
                                                                                     ggttcggTTTGATAATGCTTCTGGAAATCGGCGATTTGCTTATCGGTCAGCATCATAAGTTTCGTCGATGTACCCTGCTCGAAAGtcgctcgc    >  2:4289756/1‑93 (MQ=255)
                                                                                          |                                                                                         
GCGATAAATTTCACGGCTGTTTGGGATTTGCTCACTGTATTTTCTGTCCGGATAAATACCGGGAGGAAGAATTTGAAAACGTTCAGGTTCAGTTTGATAATGCTTCTGGAAATCGGCGATTTGCTTATCGGTCAGCATCATAAGTTTCGTCGATTTACCCTGCTCGAAAGTCGCTCGCTC  >  NC_000913/3804134‑3804313

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GGCGATAAATTTCACGGCTGTTTGGGATTTGCTCACTGTATTTTCTGTCCGGATAAATACCGGGAGGAAGAATTTGAAAACGTTCAGGTTCAGTTTGATAATGCTTCTGGAAATCGGCGATTTGCTTATCGGTCAGCATCATAAGTTTCGTCGATTTACCCTGCTCGAAAGTCGCTCGCTCAAAT  >  NC_000913/3804133‑3804317
                                                                                           |                                                                                             
GGCGATAAATTTCACGGCTGTTTGGGATTTGCTCACTGTATTTTCTGTCCGGATAAATACCGGGAGGAAGAATTTGAAAACGTTCAGGTTCGGT                                                                                             >  SRR4302543.3662216/1‑94 (MQ=60)
  CGATAAATTTCACGGCTGTTTGGGATTTGCTCACTGTATTTTCTGTCCGGCTAAATACCGGGAGGAAGAATTTGAAAACGTTCAGGTTCGGTTT                                                                                           >  SRR4302543.1987885/1‑94 (MQ=60)
  CGATAAATTTCACGGCTGTTTGGGATTTGCTCACTGTATTTTCTGTCCGGATAAATACCGGGAGGAAGAATTTGAATACGTTCAGGTTCGGTTT                                                                                           >  SRR4302543.7076589/1‑94 (MQ=60)
  CGATAAATTTCACGGCTGTTTGGGATTTGCTCACTGTATTTTCTGTCCGGATAAATACCGGGAGGAAGAATTTGAAAACGTTCAGGTTCGGTTT                                                                                           >  SRR4302543.7943068/1‑94 (MQ=60)
  CGATAAATTTCACGGCTGTTTGGGATTTGCTCACTGTATTTTCTGTCCGGATAAATACCGGGAGGAAGAATTTGAAAACGTTCAGGTTCGGTTT                                                                                           >  SRR4302543.5597010/1‑94 (MQ=60)
  CGATAAATTTCACGGCTGTTTGGGATTTGCTCACTGTATTTTCTGTCCGGATAAATACCGGGAGGAAGAATTTGAAAACGTTCAGGTTCGGTTT                                                                                           <  SRR4302543.701367/94‑1 (MQ=60)
   GATAAATTTCACGGCTGTTTGGGATTTGCTCACTGTATTTTCTGTCCGGATAAATACCGGGAGGAAGAATTTGAAAACGTTCAGGTTCGGTTTG                                                                                          <  SRR4302543.4678964/94‑1 (MQ=60)
     TAAATTTCACGGGTGTTTGGGATTTGCTCACTGTATTTTCTGTCCGGATAAATACCGGGAGGAAGAATTTGAAAACGTTCAGGTTCGGTTTGAT                                                                                        <  SRR4302543.6386254/94‑1 (MQ=60)
      AAATTTCACGGCTGTTTGGGATTTGCTCACTGTATTTTCTGTCCGGATAAATACCGGGAGGAAGAATTTGAAAACGTTCAGGTTCGGTTTGATA                                                                                       <  SRR4302543.7732436/94‑1 (MQ=60)
       AATTTCACGGCTGTTTGGGATTTGCTCACTGTATTTTCTGTCCGGATAAATACCGGGAGGAAGAATTTGAAAACGTTCAGGTTCGGTTTGATAA                                                                                      >  SRR4302543.4808825/1‑94 (MQ=60)
         TTTCACGGCTGTTTGGGATTTGCTCACTGTATTTTCTGTCCGGATAAATACCGGGAGGAAGAATTTGAAAACGTTCAGGTTCGGTTTGATAATG                                                                                    >  SRR4302543.624489/1‑94 (MQ=60)
           TCACGGCTGTTTGGGATTTGCTCACTGTATTTTCTGTCCGGATAAATACCGGGAGGAAGAATTTGAAAACGTTCAGGTTCGGTTTGATAATGCT                                                                                  <  SRR4302543.107813/94‑1 (MQ=60)
            CACGGCTGTTTGGGATTTGCTCACTGTATTTTCTGTCCGGATAAATACCGGGAGGAAGAATTTGAAAACGTTCAGGTTCGGTTTGATAATGCTT                                                                                 >  SRR4302543.3750817/1‑94 (MQ=60)
            CACGGCTGTTTGGGATTTGCTCACTGTATTTTCTGTCCGGATAAATACCGGGAGGAAGAATTTGAAAACGTTCAGGTTCGGTTTGATAATGCTT                                                                                 >  SRR4302543.259489/1‑94 (MQ=60)
            CACGGCTGTTTGGGATTTGCTCACTGTATTTTCGGTCCGGATAAATACCGGGAGGAAGAATTTGAAAACGTTCAGGTTCGGTTTGATAATGCTT                                                                                 <  SRR4302543.7709889/94‑1 (MQ=60)
              CGGCTGTTTGGGATTTGCTCACTGTATTTTCTGTCCGGATAAATACCGGGAGGAAGAATTTGAAAACGTTCAGGTTCGGTTTGATAATGCTTCT                                                                               <  SRR4302543.674149/94‑1 (MQ=60)
              CGGCTGTTTGGGATTTGCTCACTGTATTTTCTGTCCGGATAAATACCGGGAGGAAGAATTTGAAAACGTTCAGGTTCGGTTTGATAATGCTTCT                                                                               <  SRR4302543.5311405/94‑1 (MQ=60)
              CGGCTGTTTGGGATTTGCTCACTGTATTTTCTGTCCGGATAAATACCGGGAGGAAGAATTTGAAAACGTTCAGGTTCGGTTTGATAATGCTTCT                                                                               <  SRR4302543.5311406/94‑1 (MQ=60)
              CGGCTGTGTGGGATTTGCTCACTGTATTTTCTGTCCGGATAAATACCGGGAGGAAGAATTTGAAAACGTTCAGGTTCGGTTTGATAATGCTTCT                                                                               <  SRR4302543.674393/94‑1 (MQ=60)
              CAGCTGTTTGGGATTTGCTCACTGTATTTTCTGTCCGGATAAATACCGGGAGGAAGAATTTGAAAACGTTCAGGTTCGGTTTGATAATGCTTCT                                                                               <  SRR4302543.4829878/94‑1 (MQ=60)
               GGCTGTTTGGGATTTGCTCACTGTATTTTCTGTCCGGATAAATACCGGGAGGAAGAATTTGAAAACGTTCAGGTTCGGTTTGATAATGCTTCTG                                                                              >  SRR4302543.6412128/1‑94 (MQ=60)
                GCTGTTTGGGATTTGCTCACTGTATTTTCTGTCCGGATAAATACCGGGAGGAAGAATTTGAAAACGTTCAGGTTCGGTTTGATAATGCTTCTGG                                                                             >  SRR4302543.1555313/1‑94 (MQ=60)
                GCTGTTTGGGATTTGCTCACTGTATTTTCTGTCCGGATAAATACCGGGAGGAAGAATTTGAAAACGTTCAGGTTCGGTTTGATAATGCTTCTGG                                                                             <  SRR4302543.4302572/94‑1 (MQ=60)
                 CTGTTTGGGATTTGCTCACTGTATTTTCTGTCCGGATAAATACCGGGAGGAAGAATTTGAAAACGTTCAGGTTCGGTTTGATAATGCTTCTGGA                                                                            >  SRR4302543.3888100/1‑94 (MQ=60)
                 CTGTTTGGGATTTGCTCACTGTATTTTCTGTCCGGATAAATACCGGGAGGAAGAATTTGAAAACGTTCAGGTTCGGTTTGATAATGCTTCTGGA                                                                            >  SRR4302543.5438021/1‑94 (MQ=60)
                 CTGTTTGGGATTTGCTCACTGTATTTTCTGTCCGGATAAATACCGGGAGGAAGAATTTGAAAACGTTCAGGTTCGGTTTGATAATGCTTCTGGA                                                                            >  SRR4302543.5438022/1‑94 (MQ=60)
                 CTGTTTGGGATTTGCTCACTGTATTTTCTGTCCGGATAAATACCGGGAGGAAGAATTTGAAAACGTTCAGGTTCGGTTTGATAATGCTTCTGGA                                                                            >  SRR4302543.6634838/1‑94 (MQ=60)
                  TGTTTGGGATTTGCTCACTGTATTTTCTGTCCGGATAAATACCGGGAGGAAGAATTTGAAAACGTTCAGGTTCGGTTTGATACTGCTTCTGGAA                                                                           >  SRR4302543.4829873/1‑94 (MQ=60)
                  TGTTTGGGATTTGCTCACTGTATTTTCTGTCCGGATAAATACCGGGAGGAAGAATTTGAAAACGTTCAGGTTCGGTTTGATAATGCTTCTGGAA                                                                           >  SRR4302543.5625279/1‑94 (MQ=60)
                  TGTTTGGGATTTGCTCACTGTATTTTCTGTCCGGATAAATACCGGGAGGAAGAATTTGAAAACGTTCAGGTTCGGTTTGATAATGCTTCTGGAA                                                                           >  SRR4302543.479303/1‑94 (MQ=60)
                  TGTTTGGGATTTGCTCACTGTATTTTCTGTCCGGATAAATACCGGGAGGAAGAATTTGAAAACGTTCAGGTTCGGTTTGATAATGCTTCTGGAA                                                                           >  SRR4302543.4829872/1‑94 (MQ=60)
                  TGTTTGGGATTTGCTCACTGTATTTTCTGTCCGGATAAATACCGGGAGGAAGAATTTGAAAACGTTCAGGTTCGGTTTGATAATGCTTCTGGAA                                                                           >  SRR4302543.6524743/1‑94 (MQ=60)
                   GTTTGGGATTTGCTCACTGTATTTTCTGTCCGGATAAATACCGGGAGGAAGAATTTGAAAACGTTCAGGTTCGGTTTGATAATGCTTCTGGAAA                                                                          >  SRR4302543.2739180/1‑94 (MQ=60)
                   GTTTGGGATTTGCTCACTGTATTTTCTGTCCGGATAAATACCGGGAGGAAGAATTTGAAAACGTTCAGGTTCGGTTTGATAATGCTTCTGGAAA                                                                          >  SRR4302543.7313412/1‑94 (MQ=60)
                    TTTGGGATTTGCTCACTGTATTTTCTGTCCGGATAAATACCGGGAGGAAGAATTTGAAAACGTTCAGGTTCGGTTTGATAATGCTTCTGGAAAT                                                                         <  SRR4302543.5977601/94‑1 (MQ=60)
                      TGGGATTTGCTCACTGTATTTTCTGTCCGGATAAATACCGGGAGGAAGAATTTGAAAACGTTCAGGTTCGGTTTGATAATGCTTCTGGAAATCG                                                                       >  SRR4302543.7911980/1‑94 (MQ=60)
                         GATTTGCTCACTGTATTTTCTGTCCGGATAAATACCGGGAGGAAGAATTTGAAAACGTTCAGGTTCGGTTTGATAATGCTTCTGGAAATCGGCG                                                                    <  SRR4302543.934857/94‑1 (MQ=60)
                          ATTTGCTCACTGTATTTTCTGTCCGGATAAATACCGGGAGGAAGAATTTGAAAACGTTCAGGTTCGGTTTGATAATGCTTCTGGAAATCGGCGA                                                                   <  SRR4302543.1898205/94‑1 (MQ=60)
                           TTTGCTCACTGTATTTTCTGTCCGGATAAATACCGGGAGGAAGAATTTGAAAACGTTCAGGTTCGGTTTGATAATGCTTCTGGAAATCGGCGAT                                                                  <  SRR4302543.7563878/94‑1 (MQ=60)
                            TTGCTCACTGTATTTTCTGTCCGGATAAATACCGGGAGGAAGAATTTGAAAACGTTCAGGTTCGGTTTGATAATGCTTCTGGAAATCGGCGATT                                                                 <  SRR4302543.7832266/94‑1 (MQ=60)
                            TTGCTCACTGTATTTTCTGTCCGGATAAATACCGGGAGGAAGAATTTGAAAACGTTCAGGTTCGGTTTGATAATGCTTCTGGAAATCGGCGATT                                                                 >  SRR4302543.3568709/1‑94 (MQ=60)
                            TTGCTCACTGTATTTTCTGTCCGGATAAATACCGGGAGGAAGAATTTGAAAACGTTCAGGTTCGGTTTGATAATGCTTCTGGAAATCGGCGATT                                                                 >  SRR4302543.1571189/1‑94 (MQ=60)
                             TGCTCACTGTATTTTCTGTCCGGATAAATACCGGGAGGAAGAATTTGAAAACGTTCAGGTTCGGTTTGATAATGCTTCTGGAAATCGGCGATTT                                                                <  SRR4302543.5617544/94‑1 (MQ=60)
                               CTCACTGTATTTTCTGTCCGGATAAATACCGGGAGGAAGAATTTGAAAACGTTCAGGTTCGGTTTGATAATGCTTCTGGAAATCGGCGATTTGT                                                              >  SRR4302543.1360884/1‑94 (MQ=60)
                               CTCACTGTATTTTCTGTCCGGATAAATACCGGGAGGAAGAATTTGAAAACGTTCAGGTTCGGTTTGATAATGCTTCTGGAAATCGGCGATTTGC                                                              <  SRR4302543.1083457/94‑1 (MQ=60)
                               CTCACTGTATTTTCTGTCCGGATAAATACCGGGAGGAAGAATTTGAAAACGTTCAGGATCGGTTTGATAATGCTTCTGGAAATCGGCGATTTGC                                                              >  SRR4302543.352493/1‑94 (MQ=60)
                                TCACTGTATTTTCTGTCCGGATAAATACCGGGAGGAAGAATTTGAAAACGTTCAGGTTCGGTTTGATAATGCTTCTGGAAATCGGCGATTTGCT                                                             >  SRR4302543.3646128/1‑94 (MQ=60)
                                TCACTGTATTTTCTGTCCGGATAAATACCGGGAGGAAGAATTTGAAAACGTTCAGGTTCGGTTTGATAATGCTTCTGGAAATCGGCGATTTGCT                                                             >  SRR4302543.1596197/1‑94 (MQ=60)
                                TCACTGTATTTTCTGTCCGGATAAATACCGGGAGGAAGAATCTGAAAACGTTCAGGTTCGGTTTGATAATGCTTCTGGAAATCGGCGATTTGCT                                                             >  SRR4302543.5004694/1‑94 (MQ=60)
                                 CACTGTATTTTCTGTCCGGATAAATACCGGGAGGAAGAATTTGAAAACGTTCAGGTTCGGTTTGATAATGCTTCTGGAAATCGGCGATTTGCTT                                                            >  SRR4302543.6447893/1‑94 (MQ=60)
                                 CACTGTATTTTCTGTCCGGATAAATACCGGGAGGAAGAATTTGAAAACGTTCAGGTTCGGTTTGATAATGCTTCTGGAAATCGGCGATTTGCTT                                                            <  SRR4302543.3513065/94‑1 (MQ=60)
                                  ACTGTATTTTCTGTCCGGATAAATACCGGGAGGAAGAATTTGAAAACGTTCAGGTTCGGTTTGATAATGCTTCTGGAAATCGGCGATTTGCTTA                                                           >  SRR4302543.4014525/1‑94 (MQ=60)
                                  ACTGTATTTTCTGTCCGGATAAATACCGGGAGGAAGAATTTGAAAACGTTCAGGTTCGGTTTGATAATGCTTCTGGAAATCGGCGATTTGCTTA                                                           <  SRR4302543.6591335/94‑1 (MQ=60)
                                  ACTGTATTTTCTGTCCGGATAAATACCGGGAGGAAGAATTTGAAAACGTTCAGGTTCGGTTTGATAATGCTTCTGGAAATCGGCGATTTGCTTA                                                           >  SRR4302543.1411563/1‑94 (MQ=60)
                                  ACTGTATTTTCTGTCCGGATAAATACCGGGAGGAAGAATTTGAAAACGTTCAGGTTCGGTTTGATAATGCTTCTGGAAATCGGCGATTTGCTTA                                                           <  SRR4302543.5127042/94‑1 (MQ=60)
                                  ACTGTATTTTCTGTACGGATAACTACCGGGACGAAGAATTTGAAACCGTTCAGGTTCGGTTTGATAATGCTTCTGGAAATCGGCGATTTGCTTA                                                           <  SRR4302543.7843669/94‑1 (MQ=60)
                                   CTGTATTTTCTGTCCGGATAAATACCGGGAGGAAGAATTTGAAAACGTTCAGGTTCGGTTTGATAATGCTTCTGGAAATCGGCGATTTGCTTAT                                                          >  SRR4302543.7498291/1‑94 (MQ=60)
                                   CTGTATTTTCTGTCCGGATAAATACCGGGAGGAAGAATTTGAAAACGTTCAGGTTCGGTTTGATAATGCTTCTGGAAATCGGCGATTTGCTTAT                                                          >  SRR4302543.1918933/1‑94 (MQ=60)
                                   CTGTATTTTCTGTCCGGATAAATACCGGGAGGAAGAATTTGAAAACGTTCAGGTTCGGTTTGATAATGCTTCTGGAAATCGGCGATTTGCTTAT                                                          >  SRR4302543.6718676/1‑94 (MQ=60)
                                   CTGTATTTTCTGTCCGGATAAATACCGGGAGGAAGAATTTGAAAACGTTCAGGTTCGGTTTGATAATGCTTCTGGAAATCGGCGATTTGCTTAT                                                          >  SRR4302543.5899926/1‑94 (MQ=60)
                                   CTGTATTTTCTGTCCGGATAAATACCGGGAGGAAGAATTTGAAAACGTTCAGGTTCGGTTTGATAATGCTTCTGGAAATCGGCGATTTGCTTAT                                                          <  SRR4302543.3925327/94‑1 (MQ=60)
                                   CTGTATTTTCTGTCCGGATAAATACCGGGAGGAAGAATTTGAAAACGTTCAGGTTCGGTTTGATAATGCTTCTGGAAATCGGCGATTTGCTTAT                                                          >  SRR4302543.6083067/1‑94 (MQ=60)
                                   CTGTATTTTCTGTCCGGATAAATACCGGGAGGAAGAATTTGAAAACGTTCAGGTTCGGGTTGATAATGCTTCTGGAAATCGGCGATTTGCTTAT                                                          >  SRR4302543.3875529/1‑94 (MQ=60)
                                    TGTATTTTCTGTCCGGATAAATACCGGGAGGAAGAATTTGAAAACGTTCAGGTTCGGTTTGATAATGCTTCTGGAAATCGGCGATTTGCTTATC                                                         >  SRR4302543.5947575/1‑94 (MQ=60)
                                    TGTATTTTCTGTCCGGATAAATACCGGGAGGAAGAATTTGAAAACGTTCAGGTTCGGTTTGATAATGCTTCTGGAAATCGGCGATTTGCTTATC                                                         <  SRR4302543.2727557/94‑1 (MQ=60)
                                     GTATTTTCTGTCCGGATAAATACCGGGAGGAAGAATTTGAAAACGTTCAGGTTCGGTTTGATAATGCTTCTGGAAATCGGCGATTTGCTTATCG                                                        >  SRR4302543.2404800/1‑94 (MQ=60)
                                      TATTTTCTGTCCGGATAAATACCGGGAGGAAGAATTTGAAAACGTTCAGGTTCGGTTTGATAATGCTTCTGGAAATCGGCGATTTGCTTATCGG                                                       <  SRR4302543.6575868/94‑1 (MQ=60)
                                      TATTTTCTGTCCGGATAAATACCGGGAGGAAGAATTTGAAAACGTTCAGGTTCGGTTTGATAATGCTTCTGGAAATCGGCGATTTGCTTATCGG                                                       <  SRR4302543.3940113/94‑1 (MQ=60)
                                       ATTTTCTGTCCGGATAAATACCGGGAGGAAGAATTTGAAAACGTTCAGGTTCGGTTTGATAATGCTTCTGGAAATCGGCGATTTGCTTATCGGT                                                      >  SRR4302543.5349304/1‑94 (MQ=60)
                                       ATTTTCTGTCCGGATAAATACCGGGAGGAAGAATTTGAAAACGTTCAGGTTCGGTTTGATAATGCTTCTGGAAATCGGCGATTTGCTTATCGGT                                                      <  SRR4302543.3242078/94‑1 (MQ=60)
                                        TTTTCTGTCCGGATAAATACCGGGAGGAAGAATTTGAAAACGTTCAGGTTCGGTTTGATAATGCTTCTGGAAATCGGCGATTTGCTTATCGGTC                                                     <  SRR4302543.4635905/94‑1 (MQ=60)
                                         TTTCTGTCCGGATAAATACCGGGAGGAAGAATTTGAAAACGTTCAGGTTCGGTTTGATAATGCTTCTGGAAATCGGCGATTTGCTTATCGGTCA                                                    >  SRR4302543.3909884/1‑94 (MQ=60)
                                         TTTCTGTCCGGATAAATACCGGGAGGAAGAATTTGAAAACGTTCAGGTTCGGTTTGATAATGCTTCTGGAAATCGGCGATTTGCTTATCGGTCA                                                    >  SRR4302543.4479766/1‑94 (MQ=60)
                                          TTCTGTCCGGATAAATACCGGGAGGAAGAATTTGAAAACGTTCAGGTTCGGTTTGATAATGCTTCTGGAAATCGGCGATTTGCTTATCGGTCAG                                                   >  SRR4302543.5684936/1‑94 (MQ=60)
                                           TCTGTCCGGATAAATACCGGGAGGAAGAATTTGAAAACGTTCAGGTTCGGTTTGATAATGCTTCTGGAAATCGGCGATTTGCTTATCGGTCAGC                                                  >  SRR4302543.7634692/1‑94 (MQ=60)
                                           TCTGTCCGGATAAATACCGGGAGGAAGAATTTGAAAACGTTCAGGTTCGGTTTGATAATGCTTCTGGAAATCGGCGATTTGCTTATCGGTCAGC                                                  >  SRR4302543.5592082/1‑94 (MQ=60)
                                           TCTGTCCGGATAAATACCGGGAGGAAGAATTTGAAAACGTTCAGGTTCGGCTCGATAATGCTTCTTGAAATCGGCGAATTGTTTATCGGTGAGC                                                  >  SRR4302543.7634691/1‑94 (MQ=60)
                                             TGTCCGGATAAATACCGGGAGGAAGAATTTGAAAACGTTCAGGTTCGGTTTGATAATGCTTCTGGAAATCGGCGATTTGCTTATCGGTCAGCAT                                                >  SRR4302543.1514329/1‑94 (MQ=60)
                                               TCCGGATAAATACCGGGAGGAAGAATTTGAAAACGTTCAGGTTCGGTTTGATAATGCTTCTGGAAATCGGCGATTTGCTTATCGGTCAGCATCA                                              >  SRR4302543.458457/1‑94 (MQ=60)
                                               TCCGGATAAATACCGGGAGGAAGAATTTGAAAACGTTCAGGTTCGGTTTGATAATGCTTCTGGAAATCGGCGATTTGCTTATCGGTCAGCATCA                                              >  SRR4302543.5297550/1‑94 (MQ=60)
                                                CCGGATAAATACCGGGAGGAAGAATTTGAAAACGTTCAGGTTCGGTTTGATAATGCTTCTGGAAATCGGCGATTTGCTTATCGGTCAGCATCAT                                             <  SRR4302543.6080806/94‑1 (MQ=60)
                                                CCGGATAAATACCGGGAGGAAGAATTTGAAAACGTTCAGGTTCGGTTTGATAATGCTTCTGGAAATCGGCGATTTGCTTATCGGTCAGCATCAT                                             <  SRR4302543.7437415/94‑1 (MQ=60)
                                                CCGGATAAATACCGGGAGGAAGAATTTGAAAACGTTCAGGTTCGGTTTGATAATGCTTCTGGAAATCGGCGATTTGCTTATCGGTCAGCATCAT                                             <  SRR4302543.6870257/94‑1 (MQ=60)
                                                 CGGATAAATACCGGGAGGAAGAATTTGAAAACGTTCAGGTTCGGTTTGATAATGCTTCTGGAAATCGGCGATTTGCTTATCGGTCAGCATCATA                                            >  SRR4302543.674311/1‑94 (MQ=60)
                                                  GGATAAATACCGGGAGGAAGAATTTGAAAACGTTCAGGTTCGGTTTGATAATGCTTCTGGAAATCGGCGATTTGCTTATCGGTCAGCATCATAA                                           >  SRR4302543.7669070/1‑94 (MQ=60)
                                                  GGATAAATACCGGGAGGAAGAATTTGAAAACGTTCAGGTTCGGTTTGATAATGCTTCTGGAAATCGGCGATTTGCTTATCGGTCAGCATCATAA                                           <  SRR4302543.7214846/94‑1 (MQ=60)
                                                  GGATAAATACCGGGAGGAAGAATTTGAAAACGTTCAGGTTCGGTTTGATAATGCTTCTGGAAATCGGCGATTTGCTTATCGGTCAGCATCATAA                                           <  SRR4302543.260940/94‑1 (MQ=60)
                                                  GGATAAATACCGGGAGGAAGAATTTGAAAACGTTCAGGTTCGGTTTGATAATGCTTCTGGAAATCGGCGATTTGCTTATCGGTCAGCATCATAA                                           >  SRR4302543.8007405/1‑94 (MQ=60)
                                                  GGATAAATACCGGGAGGAAGAATTTGAAAACGTTCAGGTTCGGTTTGATAATGCTTCTGGAAATCGGCGATTTGCTTATCGGTCAGCATCATAA                                           >  SRR4302543.5883172/1‑94 (MQ=60)
                                                  GGATAAATACCGGGAGGAAGAATTTGAAAACGTTCAGGTTCGGTTTGATAATGCTTCTGGAAATCGGCGATTTGCTTATCGGTCAGCATCATAA                                           >  SRR4302543.5184615/1‑94 (MQ=60)
                                                   GATAAATACCGGGAGGAAGAATTTGAAAACGTTCAGGTTCGGTTTGGTAATGCTTCTGGAAATCGGCGATTTGCTTATCGGTCAGCATCATAAG                                          >  SRR4302543.5953328/1‑94 (MQ=60)
                                                    ATAAATACCGGGAGGAAGAATTTGAAAACGTTCAGGTTCGGTTTGATAATGCTTCTGGAAATCGGCGATTTGCTTATCGGTCAGCATCATAAGT                                         >  SRR4302543.7432061/1‑94 (MQ=60)
                                                    ATAAATACCGGGAGGAAGAATTTGAAAACGTTCAGGTTCGGTTTGATAATGCTTCTGGAAATCGGCGATTTGCTTATCGGTCAGCATCATAAGT                                         >  SRR4302543.120377/1‑94 (MQ=60)
                                                    ATAAATACCGGGAGGAAGAATTTGAAAACGTTCAGGTTCGGTTTGATAATGCTTCTGGAAATCGGCGATTTGCTTATCGGTCAGCATCATAAGT                                         <  SRR4302543.3329374/94‑1 (MQ=60)
                                                      AAATACCGGGAGGAAGAATTTGAAAACGTTCAGGTTCGGTTTGATAATGCTTCTGGAAATCGGCGATTTGCTTATCGGTCAGCATCATAAGTTT                                       <  SRR4302543.3395394/94‑1 (MQ=60)
                                                        ATACCGGGAGGAAGAATTTGAAAACGTTCAGGTTCGGTTTGATAATGCTTCTGGAAATCGGCGATTTGCTTATCGGTCAGCATCATAAGTTTCG                                     >  SRR4302543.6743015/1‑94 (MQ=60)
                                                         TACCGGGAGGAAGAATTTGAAAACGTTCAGGTTCGGTTTGATAATGCTTCTGGAAATCGGCGATTTGCTTATCGGTCAGCATCATAAGTTTCGT                                    <  SRR4302543.4247169/94‑1 (MQ=60)
                                                           CCGGGAGGAAGAATTTGAAAACGTTCAGGTTCGGTTTGATAATGCTTCTGGAAATCGGCGATTTGCTTATCGGTCAGCATCATAAGTTTCGTCG                                  >  SRR4302543.6694478/1‑94 (MQ=60)
                                                           CCGGGAGGAAGAATTTGAAAACGTTCAGGTTCGGTTTGATAATGCTTCTGGAAATCGGCGATTTGCTTATCGGTCAGCATCATAAGTTTCGTCG                                  <  SRR4302543.3860505/94‑1 (MQ=60)
                                                            CGGGAGGAAGAATTTGAAAACGTTCAGGTTCGGTTTGATAATGCTTCTGGAAATCGGCGATTTGCTTATCGGTCAGCATCATAAGTTTCGTCGA                                 <  SRR4302543.5078292/94‑1 (MQ=60)
                                                            CGGGAGGAAGAATTTGAAAACGTTCAGGTTCGGTTTGATAATGCTTCTGGAAATCGGCGATTTGCTTATCGGTCAGCATCATAAGTTTCGTCGA                                 >  SRR4302543.7625736/1‑94 (MQ=60)
                                                             GGGAGGAAGAATTTGAAAACGTTCAGGTTCGGTTTGATAATGCTTCTGGAAATCGGCGATTTGCTTATCGGTCAGCATCATAAGTTTCGTCGAT                                >  SRR4302543.14784/1‑94 (MQ=60)
                                                              GGAGGAATAATTTGAAAACGTTCAGGTTCGGTTTGATAATGCTTCTGGAAATCGGCGATTTGCTTATCGGTCAGCATCATAAGTTTCGTCGATT                               <  SRR4302543.3709910/94‑1 (MQ=60)
                                                              GGAGGAAGAATTTGAAAACGTTCAGGTTCGGTTTGATAATGCTTCTGGAAATCGGCGATTTGCTTATCGGTCAGCATCATAAGTTTCGTCGATT                               <  SRR4302543.7627526/94‑1 (MQ=60)
                                                              GGAGGAAGAATTTGAAAACGTTCAGGTTCGGTTTGATAATGCTTCTGGAAATCGGCGATTTGCTTATCGGTCAGCATCATAAGTTTCGTCGATT                               >  SRR4302543.2588812/1‑94 (MQ=60)
                                                              GGAGGAAGAATTTGAAAACGTTCAGGTTCGGTTTGATAATGCTTCTGGAAATCGGCGATTTGCTTATCGGTCAGCATCATAAGTTTCGTCGATT                               >  SRR4302543.5049726/1‑94 (MQ=60)
                                                               GAGGAAGAATTTGAAAACGTTCAGGTTCGGTTTGATAATGCTTCTGGAAATCGGCTATTTGCTTATCGGGCATCATCATAAGTTTCGTCGAGTT                              >  SRR4302543.4638741/1‑94 (MQ=60)
                                                               GAGGAAGAATTTGAAAACGTTCAGGTTCGGTTTGATAATGCTTCTGGAAATCGGCGATTTGCTTATCGGTCAGCATCATAAGTTTCGTCGATTT                              <  SRR4302543.5589334/94‑1 (MQ=60)
                                                                AGGAAGAATTTGAAAACGTTCAGGTTCGGTTTGATAATGCTTCTGGAAATCGGCGATTTGCTTATCGGTCAGCATCATAAGTTTCGTCGATTTA                             >  SRR4302543.6442623/1‑94 (MQ=60)
                                                                AGGAAGAATTTGAAAACGTTCAGGTTCGGTTTGATAATGCTTCTGGAAATCGGCGATTTGCTTATCGGTCAGCATCATAAGTTTCGTCGATTTA                             <  SRR4302543.6965916/94‑1 (MQ=60)
                                                                 GGAAGAATTTGAAAACGTTCAGGTTCGGTTTGATAATGCTTCTGGAAATCGGCGATTTGCTTATCGGTCAGCATCATAAGTTTCGTCGATTTAC                            >  SRR4302543.4615078/1‑94 (MQ=60)
                                                                 GGAAGAATTTGAAAACGTTCAGGTTCGGTTTGATAATGCTTCTGGAAATCGGCGATTTGCTTATCGGTCAGCATCATAAGTTTCGTCGATTTAC                            >  SRR4302543.530192/1‑94 (MQ=60)
                                                                  GAAGAATTTGAAAACGTTCAGGTTCGGTTTGATAATGCTTCTGGAAATCGGCGATTTGCTTATCGGTCAGCATCATAAGTTTCGTCGATTTACC                           >  SRR4302543.2231299/1‑94 (MQ=60)
                                                                  GAAGAATTTGAAAACGTTCAGGTTCGGTTTGATAATGCTTCTGGAAATCGGCGATTTGCTTATCGGTCAGCATCATAAGTTTCGTCGATTTACC                           <  SRR4302543.7365566/94‑1 (MQ=60)
                                                                  GAAGAATTTGAAAACGTTCAGGTTCGGTTTGATAATGCTTCTGGAAATAGGCGATTTGCTTATCGGTCAGCATCATAAGTTTCGTCGATTTACC                           >  SRR4302543.4367845/1‑94 (MQ=60)
                                                                   AAGAATTTGAAAACGTTCAGGTTCGGTTTGATAATGCTTCTGGAAATCGGCGATTTGCTTATCGGTCAGCATCATAAGTTTCGTCGATTTACCC                          <  SRR4302543.1150540/94‑1 (MQ=60)
                                                                   AAGAATTTGAAAACGTTCAGGTTCGGTTTGATAATGCTTCTGGAAATCGGCGATTTGCTTATCGGTCAGCATCATAAGTTTCGTCGATTTACCC                          >  SRR4302543.2167328/1‑94 (MQ=60)
                                                                    AGAATTTGAGAACGTTCAGGTTCGGTTTGATAATGCTTCTGGAAATCGGCGATTTGCTTATCGGTCAGCATCATAAGTTTCGTCGATTTACCCT                         <  SRR4302543.5967293/94‑1 (MQ=60)
                                                                    AGAATTTGAAAACGTTCAGGTTCGGTTTGATAATGCTTCTGGAAATCGGCGATTTGCTTATCGGTCAGCATCATAAGTTTCGTCGATTTACCCT                         >  SRR4302543.4772679/1‑94 (MQ=60)
                                                                    AGAATTTGAAAACGTTCAGGTTCGGTTTGATAATGCTTCTGGAAATCGGCGATTTGCTTATCGGTCAGCATCATAAGTTTCGTCGATTTACCCT                         <  SRR4302543.4474050/94‑1 (MQ=60)
                                                                     GAATTTGAAAACGTTCAGGTTCGGTTTGATAATGCTTCTGGAAATCGGCGATTTGCTTATCGGTCAGCATCATAAGTTTCGTCGATTTACCCTG                        <  SRR4302543.1320658/94‑1 (MQ=60)
                                                                         TTGAAAACGTTCAGGTTCGGTTTGATAATGCTTCTGGAAATCGGCGATTTGCTTATCGGTCAGCATCATAAGTTTCGTCGATTTACCCTGCTCG                    >  SRR4302543.1888359/1‑94 (MQ=60)
                                                                         TTGAAAACGTTCAGGTTCGGTTTGATAATGCTTCTGGAAATCGGCGATTTGCTTATCGGTCAGCATCATAAGTTTCGTCGATTTACCCTGCTCG                    <  SRR4302543.2008771/94‑1 (MQ=60)
                                                                            AAAACGTTCAGGTTCGGTTTGATAATGCTTCTGGCAATCGGCGATTTGCTTATCGGTCAGCATCATAAGTTTCGTCGATTTACCCTGCTCGAAA                 >  SRR4302543.4026965/1‑94 (MQ=60)
                                                                            AAAACGTTCAGGTTCGGTTTGATAATGCTTCTGGAAATCGGCGATTTGCTTATCGGTCAGCATCATAAGTTTCGTCGATTTACCCTGCTCGAAA                 >  SRR4302543.6968694/1‑94 (MQ=60)
                                                                            AAAACGTTCAGGTTCGGTTTGATAATGCTTCTGGAAATCGGCGATTTGCTTATCGGTCAGCATATTAAGTTTCGTCGATTTACCCTGCTCGAAA                 >  SRR4302543.6794076/1‑94 (MQ=60)
                                                                             AAACGTTCAGGTTCGGTTTGATAATGCTTCTGGAAATCGGCGATTTGCTTATCGGTCAGCATCATAAGTTTCGTCGATTTACCCTGCTCGAAAG                <  SRR4302543.5559217/94‑1 (MQ=60)
                                                                             AAACGTTCAGGTTCGGTTTGATAATGCTTCTGGAAATCGGCGATTTGCTTATCGGTCAGCATCATAAGTTTCGTCGATTTACCCTGCTCGAAAG                >  SRR4302543.7181494/1‑94 (MQ=60)
                                                                             AAACGTTCAGGTTCGGTTTGATAATGCTTCTGGAAATCGGCGATTTGCTTATCGGTCAGCATCATAAGTTTCGTCGATTTACCCTGCTCGAAAG                >  SRR4302543.3639622/1‑94 (MQ=60)
                                                                              AACGTTCAGGTTCGGTTTGATAATGCTTCTGGAAATCGGCGATTTGCTTATCGGTCAGCATCATAAGTTTCGTCGATTTACCCTGCTCGAAAGT               >  SRR4302543.808958/1‑94 (MQ=60)
                                                                              AACGTTCAGGTTCGGTTTGATAATGCTTCTGGAAATCGGCGATTTGCTTATCGGTCAGCATCATAAGTTTCGTCGATTTACCCTGCTCGAAAGT               >  SRR4302543.4792552/1‑94 (MQ=60)
                                                                               ACGTTCAGGTTCGGTTTGATAATGCTTCTGGAAATCGGCGATTTGCTTATCGGTCAGCATCATAAGTTTCGTCGATTTACCCTGCTCGAAAGTC              <  SRR4302543.4786361/94‑1 (MQ=60)
                                                                                CGTTCAGGTTCGGTTTGATAATGCTTCTGGAAATCGGCGATTTGCTTATCGGTCAGCATCATAAGTTTCGTCGATTTACCCTGCTCGAAAGTCG             >  SRR4302543.3691845/1‑94 (MQ=60)
                                                                                CGTTCAGGTTCGGTTTGATAATGCTTCTGGAAATCGGCGATTTGCTTATCGGTCAGCATCATAAGTTTCGTCGATTTACCCTGCTCGAAAGTCG             >  SRR4302543.780724/1‑94 (MQ=60)
                                                                                CGTTCAGGTTCGGTTTGATAATGCTTCTGGAAATCGGCGATTTGCTTATCGGTCAGCATCATAAGTTTCGTCGATTTACCCTGCTCGAAAGTCG             <  SRR4302543.1952045/94‑1 (MQ=60)
                                                                                CGTTCAGGTTCGGTTTGATAATGCTTCTGGAAATCGGCGATTTGCTTATCGGTCAGAATCATAAGTTTCGTCGATTTACCCTGCTCGAAAGTCG             >  SRR4302543.780726/1‑94 (MQ=60)
                                                                                 GTTCAGGTTCGGTTTGATAATGCTTCTGGAAATCGGCGATTTGCTTATCGGTCAGCATCATAAGTTTCGTCGATTTACCCTGCTCGAAAGTCGC            >  SRR4302543.5326002/1‑94 (MQ=60)
                                                                                  TTCAGGTTCGGTTTGATAATGCTTCTGGAAATCGGCGATTTGCTTATCGGTCAGCATCATAAGTTTCGTCGATTTACCCTGCTCGAAAGTCGCT           >  SRR4302543.6011726/1‑94 (MQ=60)
                                                                                  TTCAGGTTCGGTTTGATAATGCTTCTGGAAATCGGCGATTTGCTTATCGGTCAGCATCATAAGTTTCGTCGATTTACCCTGCTCGAAAGTCGCT           <  SRR4302543.2352841/94‑1 (MQ=60)
                                                                                  TTCAGGTTCGGTTTGATAATGCTTCTGGAAATCGGCGATTTGCTTATCGGTCAGCATCATAAGTTTCGTCGATTTACCCTGCTCGAAAGTCGCT           <  SRR4302543.180983/94‑1 (MQ=60)
                                                                                   TCAGGTTCGGTTTGATAATGCTTCTGGAAATCGGCGATTTGCTTATCGGTCAGCATCATAAGTTTCGTCGATTTACCCTGCTCGAAAGTCGCTC          >  SRR4302543.6619339/1‑94 (MQ=60)
                                                                                   TCAGGTTCGGTTTGATAATGCTTCTGGAAATCGGCGATTTGCTTATCGGTCAGCATCATAAGTTTCGTCGATTTACCCTGCTCGAAAGTCGCTC          <  SRR4302543.741998/94‑1 (MQ=60)
                                                                                   TCAGGTTCGGTTTGATAATGCTTCTGGAAATCGGCGATTTGCTTATCGGTCAGCATCATAAGTTTCGTCGATTTACCCTGCTCGAAAGTCGCTC          >  SRR4302543.6619340/1‑94 (MQ=60)
                                                                                   TCAGGTTCGGTTTGATAATGCTTCTGGAAATCGGCGATTTGCTTATCGGTCAGCATCATAAGTTTCGTCGAGTTACTCTGCTCGAAAGTCGCTC          >  SRR4302543.7113016/1‑94 (MQ=60)
                                                                                       GTTCGGTTTGATAATGCTTCTGGAAATCGGCGATTTGCTTATCGGTCAGCATCATAAGTTTCGTCGATTTACCCTGCTCGAAAGTCGCTCGCTC      >  SRR4302543.6811408/1‑94 (MQ=60)
                                                                                        TTCGGTTTGATAATGCTTCTGGAAATCGGCGATTTGCTTATCGGTCAGCATCATAAGTTTCGTCGATTTACCCTGCTCGAAAGTCGCTCGCTCA     <  SRR4302543.2809767/94‑1 (MQ=60)
                                                                                         TCGGTTTGATAATGCTTCTGGAAATCGGCGATTTGCTTATCGGTCAGCATCATAAGTTTCGTCGATTTACCCTGCTCGAAAGTCGCTCGCTCAA    >  SRR4302543.6797692/1‑94 (MQ=60)
                                                                                          CGGTTTGATAATGCTTCTGGAAATCGGCGATTTGCTTATCGGTCAGCATCATAAGTTTCGTCGATTTACCCTGCTCGAAAGTCGCTCGCTCAAA   >  SRR4302543.6665334/1‑94 (MQ=60)
                                                                                           GGTTTGATAATGCTTCTGGAAATCGGCGATTTGCTTATCGGTCAGCATCATAAGTTTCGTCGATTTACCCTGCTCGAAAGTGGCTCGCTCAAAG  >  SRR4302543.519490/1‑94 (MQ=60)
                                                                                           GGTTTGATAATGCTTCTGGAAATCGGCGATTTGCTTATCGGTCAGCATCATAAGTTTCGTCGATTTACCCTGCTCGAAAGTCGCTCGCTCAAAT  >  SRR4302543.5899994/1‑94 (MQ=60)
                                                                                           GGTTTGATAATGCTTCTGGAAATCGGCGATTTGCTTATCGGTCAGCATCATAAGTTTCGTCGATTTACCCTGCTCGAAAGTCGCTCGCTCAAAT  >  SRR4302543.1401517/1‑94 (MQ=60)
                                                                                           GGTTTGATAATGCTTCTGGAAATCGGCGATTTGCTTATCGGTCAGCATCATAAGTTTCGTCGATTTACCCTGCTCGAAAGTCGCTCGCTCAAAT  <  SRR4302543.7778010/94‑1 (MQ=60)
                                                                                           GGTTTGATAATGCTTCTGGAAATCGGCGATTTGCTTATCGGTCAGCATCATAAGTTTCGTCGATTTACCCTGCTCGAAAGTCGCTCGCTCAAAT  <  SRR4302543.1946056/94‑1 (MQ=60)
                                                                                           GGTTTGATAATGCTTCTGGAAATCGGCGATTTGCTTATCGGTCAGCATCATAAGTTTCGTCGATTTACCCTGCTCGAAAGTCGCTCGCTCAAAT  >  SRR4302543.1200247/1‑94 (MQ=60)
                                                                                           |                                                                                             
GGCGATAAATTTCACGGCTGTTTGGGATTTGCTCACTGTATTTTCTGTCCGGATAAATACCGGGAGGAAGAATTTGAAAACGTTCAGGTTCAGTTTGATAATGCTTCTGGAAATCGGCGATTTGCTTATCGGTCAGCATCATAAGTTTCGTCGATTTACCCTGCTCGAAAGTCGCTCGCTCAAAT  >  NC_000913/3804133‑3804317

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑