Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I1 R1	1696	279.3	15874187	97.8%	15524954	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,957,548	1 bp→GG	intergenic (‑117/‑744)	ppiC ← / → rep	peptidyl‑prolyl cis‑trans isomerase C/rep helicase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,957,547	1	.	G	94.7%	854.4 / 38.8	285	intergenic (‑116/‑745)	ppiC/rep	peptidyl‑prolyl cis‑trans isomerase C/rep helicase
Reads supporting (aligned to +/- strand): ref base . (9/6); new base G (132/138); total (141/144)
Fisher's exact test for biased strand distribution p-value = 4.38e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.36e-02
*	NC_000913	3,957,548	0	T	G	100.0%	902.8 / NA	285	intergenic (‑117/‑744)	ppiC/rep	peptidyl‑prolyl cis‑trans isomerase C/rep helicase
Reads supporting (aligned to +/- strand): ref base T (0/0); new base G (141/144); total (141/144)

Only 100 of 299 total aligned reads displayed.

AGCATGATCCGCAGATGCTAACGTAATTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAAT‑CC‑T‑GC‑T‑CGCAGGT‑CCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTGT  >  NC_000913/3957471‑3957627

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aGCATGATCCGCAGATGCTAACGTAATTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAAT‑CCGG‑GCTT‑CGCAGGT‑ccc                                                                    <  2:3198887/93‑2 (MQ=255)

  cATGATCCGCAGATGCTAACGTAATTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGATAAT‑CCGG‑GCTT‑CGCAGGT‑ccctc                                                                  >  2:5644126/1‑90 (MQ=255)

     gATCCGCAGATGCTAACGTAATTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAAT‑CCGG‑GCTT‑CGCAGGTCCCTCAAg                                                                <  2:3852705/93‑1 (MQ=255)

      aTCCGCAGATGCTAACGTAATTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAAT‑CCGG‑GCTT‑CGCAGGTCCCTCAAgg                                                               <  1:3947108/93‑1 (MQ=255)

      aTCCGCAGATGCTAACGTAATTGTCAGTCAGGGAGTGGCTGGGACATATATGGCAAAAGCCCGGAAAAT‑CCGG‑GCTT‑CGCAGGTCCCTCAAgg                                                               >  2:3378234/1‑93 (MQ=255)

       tCCGCAGATGCTAACGTAATTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAAT‑CCGG‑GCTT‑CGCAGGTCCCTCAAggg                                                              <  1:3178266/93‑1 (MQ=255)

       tCCGCAGATGCTAACGTAATTGTCAGTCAGGGAGTGGCTGGGACATATATGGCAAAAGCCCGGAAAAT‑CCGG‑GCTT‑CGCAGGTCCCTCAAggg                                                              >  2:5975651/1‑93 (MQ=255)

         cGCAGATGCTAACGTAATTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAAT‑CCGG‑GCTT‑CGCAGGTCCCTCAAGGGga                                                            >  1:6675462/1‑93 (MQ=255)

          gCAGATGCTAACGTAATTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAAT‑CCGG‑GCTT‑CGCAGGTCCCTCAAGGGgag                                                           >  2:364921/1‑93 (MQ=255)

           cAGATGCTAACGTAATTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAAT‑CCGG‑GCTT‑CGCAGGTCCCTCAAGGGgaga                                                          <  2:1012947/93‑1 (MQ=255)

            aGATGCTAACGTAATTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAAT‑CCGG‑GCTT‑CGCAGGTCCCTCAAGGGGAGaa                                                         <  2:7488492/93‑1 (MQ=255)

              aTGCTAACGTAATTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAAT‑CCGG‑GCTT‑CGCAGGTCCCTCAAGGGGAGaaaa                                                       <  2:4461026/93‑1 (MQ=255)

               tGCTAACGTAATTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAAT‑CCGG‑GCTT‑CGCAGGTCCCTCAAGGGGAGAAAAc                                                      >  2:2373865/1‑93 (MQ=255)

               tGCTAACGTAATTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAAT‑CCGG‑GCTT‑CGCAGGTCCCTCAAGGGGAGAAAAc                                                      >  1:7340250/1‑93 (MQ=255)

                gCTAACGTAATTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAAT‑CCGG‑GCTT‑CGCAGGTCCCTCAAGGGGAGAAAACt                                                     >  1:4931482/1‑93 (MQ=255)

                gCTAACGTAATTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAAT‑CCGG‑GCTT‑CGCAGGTCCCTCAAGGGGAGAAAACt                                                     >  1:4734322/1‑93 (MQ=255)

                 cTAACGTAATTGTCAGTCAGGGAGTGGCTGGTACATATATTGCAAAAGCCCGGAAAAT‑CCGG‑GCTT‑CGCAGGTCCCTCAAGGGGAGAAAACtt                                                    >  2:5280072/1‑93 (MQ=255)

                 cTAACGTAATTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAAT‑CCGG‑GCTT‑CGCAGGTCCCTCAAGGGGAGAAAACtt                                                    >  1:1016958/1‑93 (MQ=255)

                 cTAACGTAATTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAAT‑CCGG‑GCTT‑CGCAGGTCCCTCAAGGGGAGAAAACtt                                                    <  2:6397098/93‑1 (MQ=255)

                  tAACGTAATTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAAT‑CCGG‑GCTT‑CGCAGGTCCCTCAAGGGGAGAAAACTTa                                                   >  2:6885328/1‑93 (MQ=255)

                  tAACGTAATTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAAT‑CCGG‑GCTT‑CGCAGGTCCCTCAAGGGGAGAAAACTTa                                                   <  2:1915729/93‑1 (MQ=255)

                  caaCGTAATTGTCAGACAAGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAAT‑CCGG‑GC‑TCCGCAGGTCCCTCAAGGGGAGAAAACTTa                                                   <  1:6379891/92‑1 (MQ=255)

                   agcGTAATTGTCAGTCAGGGAGTGGCTGTTACATATATGGCAAAAGCCCGGAAAAA‑CCGG‑GCTT‑CGCAGGTCCCTCAAGGGGAGAAAACTTAg                                                  <  2:4695108/91‑1 (MQ=255)

                   aaCGTAATTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAAT‑CCGG‑GCTT‑CGCAGGTCCCTCAAGGGGAGAAAACTTAg                                                  <  2:6156502/93‑1 (MQ=255)

                   aaCGTAATTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAAT‑CCGG‑GCTT‑CGCAGGTCCCTCAAGGGGAGAAAACTTAg                                                  <  1:7862306/93‑1 (MQ=255)

                   aaCGTAATTGTCAGTCAGGGAGCGGCTGGGGCATATATGGCAAAAGCCCGGAAAAT‑CCGG‑GCTT‑CGCAGGTCCCTCAAGGGGAGAAAACTTAg                                                  >  2:331943/1‑93 (MQ=255)

                    aCGTAATTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAAT‑CCGG‑GCTT‑CGCAGGTCCCTCAAGGGGAGAAAACTTAgg                                                 <  2:2526323/93‑1 (MQ=255)

                    aCGTAATTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAAT‑CCGG‑GCTT‑CGCAGGTCCCTCAAGGGGAGAAAACTTAgg                                                 >  1:2266842/1‑93 (MQ=255)

                    aCGTAATTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAAT‑CCGG‑GCTT‑CGCAGGTCCCTCAAGGGGAGAAAACTTAgg                                                 >  2:414246/1‑93 (MQ=255)

                     cGTAATTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAAT‑CCGG‑GCTT‑CGCAGGTCCCTCAAGGGGAGAAAAATTAggg                                                >  2:3024452/1‑93 (MQ=255)

                      gTAATTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAAT‑CCGG‑GCTT‑CGCAGGTCCCTCAAGGGGAGAAAACTTAGGGc                                               >  2:6954633/1‑93 (MQ=255)

                      gTAATTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAAT‑CCGG‑GCTT‑CGCAGGTCCCTCAAGGGGAGAAAACTTAGGGc                                               <  1:2834348/93‑1 (MQ=255)

                       tAATTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAAT‑CCGG‑GCTT‑CGCAGGTCCCTCAAGGGGAGAAAACTTAGGGcc                                              <  1:823182/93‑1 (MQ=255)

                        aaTTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAAT‑CCGG‑GCTT‑CGCAGGTCCCTCAAGGGGAGAAAACTTAGGGCct                                             >  1:4623059/1‑93 (MQ=255)

                        aaTTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAAT‑CCGG‑GCTT‑CGCAGGTCCCTCAAGGGGAGAAAACTTAGGGCct                                             >  1:5152088/1‑93 (MQ=255)

                         aTTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAAT‑CCGG‑GCTT‑CGCAGGTCCCTCAAGGGGAGAAAACTTAGGGCctc                                            <  1:1187606/93‑1 (MQ=255)

                          ttGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCC‑G‑GCTT‑CGCAGGTCCCTCCAGGGGTGAAAACTTAGGGCctct                                           >  2:904281/1‑93 (MQ=255)

                          ttGTCAGTCAGGGAGTGGCTGGGACATATATGGCAAAAGCCCGGAAAAT‑CCGG‑GCTT‑CGCAGGTCCCTCAAGGGGAGAAAACTTAGGGCctct                                           >  1:1395017/1‑93 (MQ=255)

                           tGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAAT‑CCGG‑GCTT‑CGCAGGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTa                                          <  1:2973049/93‑1 (MQ=255)

                            gtcagtcaGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAAT‑CCGG‑GCTT‑CGCAGGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTAt                                         <  1:6884597/93‑1 (MQ=255)

                            gtcagtcaGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAAT‑CCGG‑GCTT‑CGCAGGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTAt                                         <  2:1025249/93‑1 (MQ=255)

                             tcagtcagGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAAT‑CCGG‑GCTT‑CGCAGGTCCCTCCAGGGGAGAAAACTTAGGGCCTCTTTg                                        >  1:6958774/1‑93 (MQ=255)

                             tcagtcagGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAAT‑CCGG‑GCTT‑CGCAGGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATg                                        <  2:4414582/93‑1 (MQ=255)

                             tcagtcagGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAAT‑CCGG‑GCTT‑CGCAGGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATg                                        <  2:705691/93‑1 (MQ=255)

                             tcagtcagGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAAT‑CCGG‑GCTT‑CGCAGGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATg                                        <  1:3865050/93‑1 (MQ=255)

                             tcagtcagGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAAT‑CCGG‑GCTT‑CGCAGGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATg                                        <  2:6714198/93‑1 (MQ=255)

                             tcagtcagGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAAT‑CC‑GGGCTT‑CGCAGGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATg                                        <  2:5383523/93‑1 (MQ=255)

                               agtcagGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAAT‑CCGG‑GCTT‑CGCAGGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATggg                                      >  2:2477588/1‑93 (MQ=255)

                                gtcagGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAAT‑CCGG‑GCTT‑CGCAGGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGc                                     <  1:5519262/93‑1 (MQ=255)

                                 tcagGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAAT‑CCGG‑GCTT‑CGCAGGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCt                                    >  1:1697314/1‑93 (MQ=255)

                                 tcagGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAAT‑CCGG‑GCTT‑CGCAGGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCt                                    <  2:6523225/93‑1 (MQ=255)

                                 tcagGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAAT‑CCGG‑GCTT‑CGCAGGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCt                                    >  1:598675/1‑93 (MQ=255)

                                  cagGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAAT‑CCGG‑GCTT‑CGCAGGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTa                                   <  2:7535113/93‑1 (MQ=255)

                                   agGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAAT‑CCGG‑GCTT‑CGCAGGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTAc                                  >  1:63430/1‑93 (MQ=255)

                                   agGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAAT‑CCGG‑GCTT‑CGCAGGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTAc                                  >  2:7179247/1‑93 (MQ=255)

                                    gggAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAAT‑CCGG‑GCTT‑CGCAGGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACa                                 <  2:226591/93‑1 (MQ=255)

                                    gggAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAAT‑CCGG‑GCTT‑CGCAGGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACa                                 >  2:2224101/1‑93 (MQ=255)

                                    gggAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAAT‑CCGG‑GCTT‑CGCAGGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACa                                 >  2:32648/1‑93 (MQ=255)

                                    gggAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAAT‑CCGG‑GCTT‑CGCAGGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACa                                 >  1:2042940/1‑93 (MQ=255)

                                     ggAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAAT‑CCGG‑GCTT‑CGCAGGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACaa                                <  1:7429987/93‑1 (MQ=255)

                                      gAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAAT‑CCGG‑GCTT‑CGCAGGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACaaa                               <  2:1215628/93‑1 (MQ=255)

                                      gAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAAT‑CCGG‑GCTT‑CGCAGGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACaaa                               <  2:2873017/93‑1 (MQ=255)

                                         tGGCTGGTACATATATGGCAAAAGCCCGGAAAAT‑CC‑GGGCTT‑CGCAGGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTc                            >  1:5772427/1‑93 (MQ=255)

                                          ggCTGGTACATATATGGCAAAAGCCCGGAAAAT‑CCGG‑GCTT‑CGCAGGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCa                           <  2:1539195/93‑1 (MQ=255)

                                           gCTGGTACATATATGGTAAAAGCCCGGAAAAT‑CCGG‑GCTT‑CGCAGGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCAt                          <  2:5179412/93‑1 (MQ=255)

                                           gCTGGTACATATATGGCAAAAGCCCGGAAAAT‑CCGG‑GCTT‑CGCAGGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCAt                          <  2:5701422/93‑1 (MQ=255)

                                           gCTGGTACATATATGGCAAAAGCCCGGAAAAT‑CCGG‑GCTT‑CGCAGGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCAt                          >  1:5268562/1‑93 (MQ=255)

                                            cTGGTACATATATGGCAAAAGCCCGGAAAAT‑CCGG‑GCTT‑CGCAGGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCAtt                         <  1:1307521/93‑1 (MQ=255)

                                            cTGGTACATATATGGCAAAAGCCCGGAAAAT‑CCGG‑GCTT‑CGCAGGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCAtt                         <  1:448682/93‑1 (MQ=255)

                                             tGGTACATATATGGCAAAAGCCCGGGAAAT‑CCGG‑GCTT‑CGCAGGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTa                        <  2:5135937/93‑1 (MQ=255)

                                             tGGTACATATATGGCAAAAGCCCGGAAAAT‑CCGG‑GCTT‑CGCAGGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTa                        <  1:2390388/93‑1 (MQ=255)

                                              ggTACATATATGGCAAAAGCCCGGAAAAT‑CCGG‑GCTT‑CGCAGGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTAt                       <  1:7142401/93‑1 (MQ=255)

                                              ggTACATATATGGCAAAAGCCCGGAAAAT‑CCGG‑GCTT‑CGCAGGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTAt                       <  1:6165937/93‑1 (MQ=255)

                                                tACATATATGGCAAAAGCCCGGAAAAT‑CCGG‑GCTT‑CGCAGGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCg                     <  2:6243400/93‑1 (MQ=255)

                                                tACATATACGGCAAAAGCCCGGAAAAT‑CCGG‑GCTT‑CGCAGGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCg                     <  1:5086729/93‑1 (MQ=255)

                                                 aCATATATGGAAAAAGCCGGGAAAAT‑CCGG‑GCTT‑CGCAGGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCgg                    <  2:2836171/93‑1 (MQ=255)

                                                  cATATATGGCAAAAGCCCGGAAAAT‑CCGG‑GCTT‑CGCAGGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCggg                   >  1:7687587/1‑93 (MQ=255)

                                                   atatatGGCAAAAGCCCGGAAAAT‑CCGG‑GCTT‑CGCAGGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCgggg                  >  2:2760747/1‑93 (MQ=255)

                                                    tatatGGCAAAAGCCCGGAAAAT‑CCGG‑GCTT‑CGCAGGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGa                 >  2:1704657/1‑93 (MQ=255)

                                                    tatatGGCAAAAGCCCGGAAAAT‑CCGG‑GCTT‑CGCAGGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGa                 >  1:3093287/1‑93 (MQ=255)

                                                      tatGGCAAAAGCCCGGAAAAT‑CCGG‑GCTT‑CGCAGGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATg               >  1:1103232/1‑93 (MQ=255)

                                                      tatGGCAAAAGCCCGGAAAAT‑CCGG‑GCTT‑CGCAGGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATg               >  1:3033570/1‑93 (MQ=255)

                                                        tGGCAAAAGCCCGGAAAAT‑CCGG‑GCTT‑CGCAGGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGt             <  2:5548878/93‑1 (MQ=255)

                                                        tGGCAAAAGCCCGGAAAAT‑CCGG‑GCTT‑CGCAGGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGt             >  2:7609268/1‑93 (MQ=255)

                                                          gCAAAAGCCCGGAAAAT‑CCGG‑GCTT‑CGCAGGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGtta           >  2:4933352/1‑93 (MQ=255)

                                                           cAAAAGCCGGGAAAAT‑CCGG‑GCTT‑CGCAGGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGttat          <  1:5569400/93‑1 (MQ=255)

                                                           cAAAAGCCCGGAAAAT‑CCGG‑GCTT‑CGCAGGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGttat          <  1:4368706/93‑1 (MQ=255)

                                                            aaaaGCCCGGAAAAT‑CCGG‑GCTT‑CGCAGGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGttatt         <  2:2131257/93‑1 (MQ=255)

                                                            aaaaGCCCGGAAAAT‑CCGG‑GCTT‑CGCAGGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGttatt         >  1:7402266/1‑93 (MQ=255)

                                                            aaaaGCCCGGAAAAT‑CC‑GGGCTT‑CGCAGGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGttatt         >  1:1146561/1‑93 (MQ=255)

                                                            aaaaGCCCGGAAAAT‑CC‑GGGCTT‑CGCAGGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCACTATCGGGGATGGttatt         >  2:7013300/1‑93 (MQ=255)

                                                             aaaGCCCGGAAAAT‑CCGG‑GCTT‑CGCAGGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGttattt        >  1:4037987/1‑93 (MQ=255)

                                                             aaaGCCCGGAAAAT‑CCGG‑GCTT‑CGCAGGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGttattt        >  2:3104555/1‑93 (MQ=255)

                                                               aGCCCGGAAAAT‑CCGG‑GCTT‑CGCAGGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGttatttta      <  2:4901037/93‑1 (MQ=255)

                                                                gCCCGGAAAAT‑CCGG‑GCTT‑CGCAGGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGttattttat     >  2:6476595/1‑93 (MQ=255)

                                                                 cccGGAAAAT‑CCGG‑GCTT‑CGCAGGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGttattttatt    <  2:1144544/93‑1 (MQ=255)

                                                                  ccGGAAAAT‑CCGG‑GCTT‑CGCAGGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTg   >  2:4046988/1‑93 (MQ=255)

                                                                  ccGGAAAAT‑CCGG‑GCTT‑CGCAGGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTg   >  1:2567450/1‑93 (MQ=255)

                                                                  ccGGAAAAT‑CCGG‑GCTT‑CGCAGGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTg   >  1:1568677/1‑93 (MQ=255)

                                                                  ccGGAAAAT‑CCGG‑GCTT‑CGCAGGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTg   <  2:7837778/93‑1 (MQ=255)

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AGCATGATCCGCAGATGCTAACGTAATTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAAT‑CC‑T‑GC‑T‑CGCAGGT‑CCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTGT  >  NC_000913/3957471‑3957627

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 19 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A