Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 1696 279.3 15874187 97.8% 15524954 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,061,961 Δ1 bp coding (1386/1458 nt) b3876 ← putative permease

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,061,9590C.99.5% 1625.8 / ‑1.0 361coding (1388/1458 nt)b3876putative permease
Reads supporting (aligned to +/- strand):  ref base C (2/0);  new base . (207/152);  total (209/152)
Fisher's exact test for biased strand distribution p-value = 5.11e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.92e-01

Only 100 of 386 total aligned reads displayed.

TTTTTCATAATAAGTCCATTTTTTAAACGTAGGGAGGCCGCCACCCACCAGGATGGAAATAGCGGCTATTGACATTTATAACGTTACGGAAGCCCGTTTTTTCTTCTGTCGCCATCTCTTTATTTTTGCGTTGGTTGATTTCCTCAATAATCAATGCAAACCGTTTTTCATTGAGTGTGTA  >  NC_000913/4061867‑4062047
                                                                                            |                                                                                        
tttttCATAATAAGTCCATTTTTTAAACGTAGGGAGGCCGCCACCCACCAGGATGGAAATAGCGGCTATTGACATTTATAACGTTACGGAAGc                                                                                          >  1:93462/1‑93 (MQ=255)
tttttCATAATAAGTCCATTTTTTAAACGTAGGGAGGCCGCCACCCACCAGGATGGAAATAGCGGCTATTGACATTTATAACGTTACGGAAGc                                                                                          >  2:153963/1‑93 (MQ=255)
tttttCATAATAAGTCCATTTTTTAAACGTAGGGAGGCCGCCACCCACCAGGATGGAAATAGCGGCTATTGACATTTATAACGTTACGGAAGc                                                                                          <  2:4255504/93‑1 (MQ=255)
 ttttCATAATAAGTCCATTTTTTAAACGTAGGGAGGCCGCCACCCACCAGGATGGAAATAGCGGCTATTGACATTTATAACGTTACGGAAGcc                                                                                         >  2:7282105/1‑93 (MQ=255)
  tttCATAATAAGTCCATTTTTTAAACGTAGGGAGGCCGCCACCCACCAGGATGGAAATAGCGGCTATTGACATTTATAACGTTACGGAAGccg                                                                                        >  2:7672075/1‑92 (MQ=255)
     cATAATAAGTCCATTTTTTAAACGTAGGGAGGCCGCCACCCACCAGGATGGAAATAGCGGCTATTGACATTTATAACGTTACGGAAGccgttt                                                                                     >  1:2459635/1‑89 (MQ=255)
      ataataAGTCCATTTTTTAAACGTAGGGAGGCCGCCACCCACCAGGATGGAAATAGCGGCTATTGACATTTATAACGTTACGGAAG‑CCGtttt                                                                                   >  1:6642425/1‑93 (MQ=255)
      ataataAGTCCATTTTTTAAACGTAGGGAGGCCGCCACCCACCAGGATGGAAATAGCGGCTATTGACATTTATAACGTTACGGAAG‑CCGtttt                                                                                   >  2:3763694/1‑93 (MQ=255)
       taataaGTCCATTTTTTAAACGTAGGGAGGCCGCCACCCACCAGGATGGAAATAGCGGCTATTGACATTTATAACGTTACGGAAG‑CCGttttt                                                                                  >  1:6209882/1‑93 (MQ=255)
       taataaGTCCATTTTTTAAACGTAGGGAGGCCGCCACCCACCAGGATGGAAATAGCGGCTATTGACATTTATAACGTTACGGAAG‑CCGttttt                                                                                  <  1:2278168/93‑1 (MQ=255)
        aataaGTCCATTTTTTAAACGTAGGGAGGCCGCCACCCACCAGGATGGAAATAGCGGCTATTGACATTTATAACGTTACGGAAG‑CCGtttttt                                                                                 >  1:7134570/1‑93 (MQ=255)
         ataaGTCCATTTTTTAAACGTAGGGAGGCCGCCACCCACCAGGATGGAAATAGCGGCTATTGACATTTATAACGTTACGGAAGCCGTtttttt                                                                                 >  2:2876348/1‑93 (MQ=255)
         ataaGTCCATTTTTTAAACGTAGGGAGGCCGCCACCCACCAGGATGGAAATAGCGGCTATTGACATTTATAACGTTACGGAAG‑CCGTTTTttc                                                                                <  2:4701480/93‑1 (MQ=255)
         ataaGTCCATTTTTTAAACGTAGGGAGGCCGCCACCCACCAGGATGGAAATAGCGGCTATTGACATTTATAACGTTACGGAAG‑CCGTTTTttc                                                                                <  2:827807/93‑1 (MQ=255)
         ataaGTCCATTTTTTAAACGTAGGGAGGCCGCCACCCACCAGGATGGAAATAGCGGCTATTGACATTTATAACGTTACGGAAG‑CCGTTTTttc                                                                                <  1:6392545/93‑1 (MQ=255)
         ataaGTCCATTTTTTAAACGTAGGGAGGCCGCCACCCACCAGGATGGAAATAGCGGCTATTGACATTTAAAACGTTACGGAAG‑CCGTTTTttc                                                                                >  2:5647744/1‑93 (MQ=255)
         ataaGTCCATTTTTTAAACGTAGGGAGGACGCCACCCACCAGGATGGAAATAGCGGCTATTGACATTTATAACGTTACGGAAGCCGTtttttt                                                                                 >  2:3315293/1‑93 (MQ=255)
          taaGTCCATTTTTTAAACGTAGGGAGGCCGCCACCCACCAGGATGGAAATAGCGGCTATTCACATTTATAACGTTACGGAAG‑CCGTTTTttct                                                                               >  1:2707564/1‑93 (MQ=255)
           aaGTCCATTTTTTAAACGTAGGGAGGCCGCCACCCACCAGGATGGAAATAGCGGCTATTGACATTTATAACGTTACGGAAG‑CCGTTTTttctt                                                                              >  1:1098272/1‑93 (MQ=255)
            aGTCCATTTTTTAAACGTAGGGAGGCCGCCACCCACCAGGATGGAAATAGCGGCTATTGACATTTATAACGTTACGGAAG‑CCGTTTTttcttc                                                                             <  1:6686118/93‑1 (MQ=255)
            aGTCCATTTTTTAAACGTAGGGAGGCCGCCACCCACCAGGATGGAAATAGCGGCTATTGACATTTATAACGTTACGGAAG‑CCGTTTTttcttc                                                                             <  2:514147/93‑1 (MQ=255)
             gTCCATTTTTTAAACGTAGGGAGGCCGCCACCCACCAGGATGGAAATAGCGGCTATTGACATTTATAACGTTACGGAAG‑CCGTTTTTtcttct                                                                            <  2:4006966/93‑1 (MQ=255)
              tCCATTTTTTAAACGTAGGGAGGCCGCCACCCACCAGGATGGAAATAGCGGCTATTGACATTTATAACGTTACGGAAG‑CCGTTTTTTCTTCTg                                                                           >  2:490018/1‑93 (MQ=255)
                cATTTTTTAAACGTAGGGAGGCCGCCACCCACCAGGATGGAAATAGCGGCTATTGACATTTATAACGTTACGGAAG‑CCGTTTTTTCTTCTGTc                                                                         <  2:4717724/93‑1 (MQ=255)
                 aTTTTTTAAACGTAGGGAGGCCGCCACCCACCAGGATGGAAATAGCGGCTATTGACATTTATAACGTTACGGAAG‑CCGTTTTTTCTTCTGTCg                                                                        >  2:5456618/1‑93 (MQ=255)
                 aTTTTTTAAACGTAGGGAGGCCGCCACCCACCAGGATGGAAATAGCGGCTATTGACATTTATAACGTTACGGAAG‑CCGTTTTTTCTTCTGTCg                                                                        >  2:3107417/1‑93 (MQ=255)
                  ttttttAAACGTAGGGAGGCCGCCACCCACCAGGATGGAAATAGCGGCTATTGACATTTATAACGTTACGGAAG‑CCGTTTTTTCTTCTGTCGc                                                                       <  1:7896685/93‑1 (MQ=255)
                    ttttAAACGTAGGGAGGCCGCCACCCACCAGGATGGAAATAGCGGCTATTGACATTTATAACGTTACGGAAG‑CCGTTTTTTCTTCTGTCGCCa                                                                     >  2:6478898/1‑93 (MQ=255)
                    ttttAAACGTAGGGAGGCCGCCACCCACCAGGATGGAAATAGCGGCTATTGACATTTATAACGTTACGGAAG‑CCGTTTTTTCTTCTGTCGCCa                                                                     >  2:2134078/1‑93 (MQ=255)
                    ttttAAACGTAGGGAGGCCGCCACCCACCAGGATGGAAATAGCGGCTATTGACATTTATAACGTTACGGAAG‑CCGTTTTTTCTTCTGTCGCCa                                                                     >  2:4568610/1‑93 (MQ=255)
                     tttAAACGTAGGGAGGCCGCCACCCACCAGGATGGAAATAGCGGCTATTGACATTTATAACGTTACGGAAG‑CCGTTTTTTCTTCTGTCGCCAt                                                                    >  2:4200984/1‑93 (MQ=255)
                      ttAAACGTAGGGAGGCCGCCACCCACCAGGATGGAAATAGCGGCTATTGACATTTATAACGTTACGGAAG‑CCGTTTTTTCTTCTGTCGCCAtc                                                                   <  1:611111/93‑1 (MQ=255)
                        aaaCGTAGGGAGGCCGCCACCCACCAGGATGGAAATAGCGGCTATTGACATTTATAACGTTACGGAAG‑CCGTTTTTTCTTCTGTCGCCAtctc                                                                 <  1:1039898/93‑1 (MQ=255)
                        aaaCGTAGGGAGGCCGCCACCCACCAGGATGGAAATAGCGGCTATTGACATTTATAACGTTACGGAAG‑CCGTTTTTTCTTCTGTCGCCAtctc                                                                 <  2:3828254/93‑1 (MQ=255)
                         aaCGTAGGGAGGCCGCCACCCACCAGGATGGAAATAGCGGCTATTGACATTTATAACGTTACGGAAG‑CCGTTTTTTCTTCTGTCGCCATctct                                                                >  2:387178/1‑93 (MQ=255)
                         aaCGTAGGGAGGCCGCCACCCACCAGGATGGAAATAGCGGCTATTGACATTTATAACGTTACGGAAG‑CCGTTTTTTCTTCTGTCGCCATctct                                                                <  1:6893391/93‑1 (MQ=255)
                            gTAGGGAGGCCGCCACCCACCAGGATGGAAATAGCGGCTATTGACATTTATAACGTTACGGAAG‑CCGTTTTTTCTTCTGTCGCCATCTCTTTa                                                             >  1:4764083/1‑93 (MQ=255)
                             tAGGGAGGCCGCCACCCACCAGGATGGAAATAGCGGCTATTGACATTTATAACGTTACGGAAG‑CCGTTTTTTCTTCTGTCGCCATCTCTTTAt                                                            >  1:5022263/1‑93 (MQ=255)
                             tAGGGAGGCCGCCACCCACCAGGATGGAAATAGCGGCTATTGACATTTATAACGTTACGGAAG‑CCGTTTTTTCTTCTGTCGCCATCTCTTTAt                                                            >  1:4337127/1‑93 (MQ=255)
                             tAGGGAGGCCGCCACCCACCAGGATGGAAATAGCGGCTATTGACATTTATAACGTTACGGAAG‑CCGTTTTTTCTTCTGTCGCCATCTCTTTAt                                                            >  2:1387157/1‑93 (MQ=255)
                             tAGGGAGGCCGCCACCCACCAGGATGGAAATAGCGGCTATTGACATTTATAACGTTACGGAAG‑CCGTTTTTTCTTCTGTCGCCATCTCTTTAt                                                            <  2:7475691/93‑1 (MQ=255)
                             tAGGGAGGCCGCCACCCACCAGGATGGAAATAGCGGCTATTGACATTTATAACGTTACGGAAG‑CCGTTTTTTCTTCTGTCGCCATCTCTTTAt                                                            <  1:7668885/93‑1 (MQ=255)
                              aGGGAGGCCGCCACCCACCAGGATGGAAATAGCGGCTATTGACATTTATAACGTTACGGAAG‑CCGTTTTTTCTTCTGTCGCCATCTCTTTAtt                                                           >  2:3516477/1‑93 (MQ=255)
                              aGGGAGGCCGCCACCCACCAGGATGGAAATAGCGGCTATTGACATTTATAACGTTACGGAAG‑CCGTTTTTTCTTCTGTCGCCATCTCTTTAtt                                                           <  1:3949582/93‑1 (MQ=255)
                                ggAGGCCGCCACCCACCAGGATGGAAATAGCGGCTATTGACATTTATAACGTTACGGAAG‑CCGTTTTTTCTTCTGTCGCCATCTCTTTAtttt                                                         <  1:7407569/93‑1 (MQ=255)
                                      cgccACCCACCAGGATGGAAATAGCGGCTATTGACATTTATAACGTTACGGAAG‑CCGTTTTTTCTTCTGTCGCCATCTCTTTATTTTTGCgtt                                                   <  1:2999517/93‑1 (MQ=255)
                                      cgccACCCACCAGGATGGAAATAGCGGCTATTGACATTTATAACGTTACGGAAG‑CCGTTTTTTCTTCTGTCGCCATCTCTTTATTTTTGCgtt                                                   <  2:2712421/93‑1 (MQ=255)
                                       gccACCCACCAGGATGGAAATAGCGGCTATTGACATTTATAACGTTACGGAAG‑CCGTTTTTTCTTCTGTCGCCATCTCTTTATTTTTGCgttg                                                  >  1:5329197/1‑93 (MQ=255)
                                        ccacccacCAGGATGGAAATAGCGGCTATTGACATTTATAACGTTACGGAAG‑CCGTTTTTTCTTCTGTCGCCATCTCTTTATTTTTGCgttgg                                                 <  1:4651021/93‑1 (MQ=255)
                                        ccacccacCAGGATGGAAATAGCGGCTATTGACATTTATAACGTTACGGAAG‑CCGTTTTTTCTTCTGTCGCCATCTCTTTATTTTTGCgttgg                                                 >  1:2644776/1‑93 (MQ=255)
                                         cacccaccAGGATGGAAATAGCGGCTATTGACATTTATAACGTTACGGAAG‑CAGTTTTTTCTTCTGTCGCCATCTCTTTCTTTTCGCgttggt                                                >  1:284357/1‑93 (MQ=255)
                                          ccccaccAGGATGGAAATAGCGGCTATTGACATTTATAACGTTACGGAAG‑CCGTTTTTTCTTCTGTCGCCATCTCTTTATTTTTGCgttggtt                                               <  2:364492/92‑1 (MQ=255)
                                          acccaccAGGATGGAAATAGCGGCTATTGACATTTATAACGTTACGGAAG‑CCGTTTTTTCTTCTGTCGCCATCTCTTTATTTTTGCgttggtt                                               >  1:1795379/1‑93 (MQ=255)
                                              accaGGATGGAAATAGCGGCTATTGACATTTATAACGTTACGGAAG‑CCGTTTTTTCTTCTGTCGCCATCTCTTTATTTTTGCGTTGGTTGAtt                                           <  1:3869661/93‑1 (MQ=255)
                                              accaGGATGGAAATAGCGGCTATTGACATTTATAACGTTACGGAAG‑CCGTTTTTTCTTCTGTCGCCATCTCTTTATTTTTGCGTTGGTTGAtt                                           >  2:5554345/1‑93 (MQ=255)
                                               ccaGGATGGAAATAGCGGCTATTGACATTTATAACGTTACGGAAG‑CCGTTTTTTCTTCTGTCGCCATCTCTTTATTTTTGCGTTGGTTGAttt                                          >  1:3297099/1‑93 (MQ=255)
                                               ccaGGATGGAAATAGCGGCTATTGACATTTATAACGTTACGGAAG‑CCGTTTTTTCTTCTGTCGCCATCTCTTTATTTTTGCGTTGGTTGAttt                                          >  2:4156685/1‑93 (MQ=255)
                                               ccaGGATGGAAATAGCGGCTATTGACATTTATAACGTTACGGAAG‑CCGTTTTTTCTTCTGTCGCCATCTCTTTATTTTTGCGTTGGTTGAttt                                          >  1:548077/1‑93 (MQ=255)
                                                 aGGATGGAAATAGCGGCTATTGACATTTATAACGTTACGGAAG‑CCGTTTTTTCTTCTGTCGCCATCTCTTTATTTTTGCGTTGGTTGATTTcc                                        <  2:2459919/93‑1 (MQ=255)
                                                  ggATGGAAATAGCGGCTATTGACATTTATAACGTTACGGAAG‑CCGTTTTTTCTTCTGTCGCCATCTCTTTATTTTTGCGTTGGTTGATTTCCt                                       >  1:3730255/1‑93 (MQ=255)
                                                   gATGGAAATAGCGGCTATTGACATTTATAACGTTACGGAAG‑CCGTTTTTTCTTCTGTCGCCATCTCTTTATTTTTGCGTTGGTTGATTTCCTc                                      >  2:319994/1‑93 (MQ=255)
                                                    aTGGAAATAGCGGCTATTGACATTTATAACGTTACGGAAG‑CCGTTTTTTCTTCTGTCGCCATCTCTTTATTTTTGCGTTGGTTGATTTCCTCa                                     >  1:4240229/1‑93 (MQ=255)
                                                       gAAATAGCGGCTATTGACATTTATAACGTTACGGAAG‑CCGTTTTTTCTTCTGTCGCCATCTCTTTATTTTTGCGTTGGTTGATTTCCTCaata                                  >  2:7112191/1‑93 (MQ=255)
                                                       gAAATAGCGGCTATTGACATTTATAACGTTACGGAAG‑CCGTTTTTTCTTCTGTCGCCATCTCTTTATTTTTGCGTTGGTTGATTTCCTCaata                                  <  1:5265809/93‑1 (MQ=255)
                                                         aaTAGCGGCTATTGACATTTATAACGTTACGGAAG‑CCGTTTTTTCTTCTGTCGCCATCTCTTTATTTTTGCGTTGGTTGATTTCCTCaataat                                >  1:2437770/1‑93 (MQ=255)
                                                          aTAGCGGCTATTGACATTTATAACGTTACGGAAG‑CCGTTTTTTCTTCTGTCGCCATCTCTTTATTTTTGCGTTGGTTGATTTCCTCAATAATc                               >  1:2782606/1‑93 (MQ=255)
                                                           tAGCGGCTATTGACATTTATAACGTTACGGAAG‑CCGTTTTTTCTTCTGTCGCCATCTCTTTATTTTTGCGTTGGTTGATTTCCTCAATAATCa                              >  2:2562246/1‑93 (MQ=255)
                                                           tAGCGGCTATTGACATTTATAACGTTACGGAAG‑CCGTTTTTTCTTCTGTCGCCATCTCTTTATTTTTGCGTTGGTTGATTTACTCAATAATCa                              >  1:540185/1‑93 (MQ=255)
                                                             gCGGCTATTGACATTTATAACGTTACGGAAG‑CCGTTTTTTCTTCTGTCGCCATCTCTTTATTTTTGCGTTGGTTGATTTCCTCAATAATCAAt                            <  2:2615044/93‑1 (MQ=255)
                                                               ggCTATTGACATTTATAACGTTACGGAAG‑CCGTTTTTTCTTCTGTCGCCATCTCTTTATTTTTGCGTTGGTTGATTTCCTCAATAATCAATGc                          <  1:3579671/93‑1 (MQ=255)
                                                               ggCTATTGACATTTATAACGTTACGGAAG‑CCGGTTTTTCTTCTGTCGCCATCTCTTTATTTTTGCGTTGGTTGATTTCCTCAATAATCAATGc                          >  2:347022/1‑93 (MQ=255)
                                                                gCTATTGACATTTATAACGTTACGGAAG‑CCGTTTTTTCTTCTGTCGCCATCTCTTTATTTTTGCGTTGGTTGATTTCCTCAATAATCAATGCa                         >  1:4224765/1‑93 (MQ=255)
                                                                  tATTGACATTTATAACGTTACGGAAG‑CCGTTTTTTCTTCTGTCGCCATCTCTTTATTTTTGCGTTGGTTGATTTCCTCAATAATCAATGCaaa                       >  1:3418316/1‑93 (MQ=255)
                                                                  tATTGACATTTATAACGTTACGGAAG‑CCGTTTTTTCTTCTGTCGCCATCTCTTTATTTTTGCGTTGGTTGATTTCCTCAATAATCAATGCaaa                       >  2:5915245/1‑93 (MQ=255)
                                                                  tATTGACATTTATAACGTTACGGAAG‑CCGTTTTTTCTTCTGTCGCCATCTCTTTATTTTTGCGTTGGTTGATTTCCTCAATAATCAATGCaaa                       >  1:1436377/1‑93 (MQ=255)
                                                                  tATTGACATTTATAACGTTACGGAAG‑CCGTTTTTTCTTCTGTCGCCATCTCTTTATTTTTGCGTTGGTTGATTTCCTCAATAATCAATGCaaa                       >  2:7828911/1‑93 (MQ=255)
                                                                  tATTGACATTTATAACCTTACGGAAG‑CCGTTTTTTCTTCTGTCGCCATCTCTTTATTTTTGCGTTGGTTGATTTCCTCAATAATCAATGCaaa                       <  2:1653840/93‑1 (MQ=255)
                                                                   aTTGACATTTATAACGTTACGGAAG‑CCGTTTTTTCTTCTGTCGCCATCTCTTTATTTTTGCGTTGGTTGATTTCCTCAATAATCAATGCAAAc                      >  1:6441851/1‑93 (MQ=255)
                                                                    ttGACATTTATAACGTTACGGAAG‑CCGTTTTTTCTTCTGTCGCCATCTCTTTATTTTTGCGTTGGTTGATTTCCTCAATAATCAATGCAAAcc                     >  2:2030227/1‑93 (MQ=255)
                                                                    ttGACATTTATAACGTTACGGAAG‑CCGTTTTTTCTTCTGTCGCCATCTCTTTATTTTTGCGTTGGTTGATTTCCTCAATAATCAATGCAAAcc                     >  2:2246345/1‑93 (MQ=255)
                                                                    ttGACATTTATAACGTTACGGAAG‑CCGTTTTTTCTTCTGTCGCCATCTCTTTATTTTTGCGTTGGTTGATTTCCTCAATAATCAATGCAAAcc                     >  1:164940/1‑93 (MQ=255)
                                                                    ttGACATTTATAACGTTACGGAAG‑CCGTTTTTTCTTCTGTCGCCATCTCTTTATTTTTGCGTTGGTTGATTTCCTCAATAATCAATGCAAAcc                     >  2:6909783/1‑93 (MQ=255)
                                                                    ttGACATTTATAACGTTACGGAAG‑CCGTTTTTTCTTCTGTCGCCATCTCTTTATTTTTGCGTTGGTTGATTTCCCCAATAATCAATGCaaccg                     >  1:4553855/1‑90 (MQ=255)
                                                                     tGACATTTATAACGTTACGGAAG‑CCGTTTTTTCTTCTGTCGCCCTCTCTTTATTTTTGCGTTGGTTGATTTCCTCAATAATCAATGCAAACCg                    >  1:4906081/1‑93 (MQ=255)
                                                                      gACATTTATAACGTTACGGAAG‑CCGTTTTTTCTTCTGTCGCCATCTCTTTATTTTTGCGTTGGTTGATTTCCTCAATAATCAATGCAAACCGt                   >  2:1295293/1‑93 (MQ=255)
                                                                         aTTTATAACGTTACGGAAG‑CCGTTTTTTCTTCTGTCGCCATCTCTTTATTTTTGCGTTGGTTGATTTCCTCAATAATCAATGCAAACCGtttt                <  1:3479697/93‑1 (MQ=255)
                                                                          tttATAACGTTACGGAAG‑CCGTTTTTTCTTCTGTCGCCATCTCTTTATTTTTGCGTTGGTTGATTTCCTCAATAATCAATGCAAACCGttttt               >  2:1829741/1‑93 (MQ=255)
                                                                          tttATAACGTTACGGAAG‑CCGTTTTTTCTTCTGTCGCCATCTCTTTATTTTTGCGTTGGTTGATTTCCTCAATAATCAATGCAAACCGttttt               >  2:6403015/1‑93 (MQ=255)
                                                                           ttATAACGTTACGGAAG‑CCGTTTTTTCTTCTGTCGCCATCTCTTTATTTTTGCGTTGGTTGATTTCCTCAATAATCAATGCAAACCGTTTTTc              <  2:5810368/93‑1 (MQ=255)
                                                                           ttATAACGTTACGGAAG‑CCGCTTTTTCTTCTGTCGCCATCTCTTTATTTTTGCGTTGGTTGATTTCCTCCATAATCAATGCAAACCGTTTTTc              >  2:6081525/1‑93 (MQ=255)
                                                                            tataACGTTACGGAAG‑CCGTTTTTTCTTCTGTCGCCATCTCTTTATTTTTGCGTTGGTTGATTTCCTCAATAATCAATGCAAACCGTTTTTCa             <  1:823962/93‑1 (MQ=255)
                                                                               aaCGTTACGGAAG‑CCGTTTTTTCTTCTGTCGCCATCTCTTTATTTTTGCGTTGGTTGATTTCCTCAATAATCAATGCAAACCGTTTTTCATTg          <  1:5699828/93‑1 (MQ=255)
                                                                               aaCGTTACGGAAG‑CCGTTTTTTCTTCTGTCGCCATCTCTTTATTTTTGCGTTGGTTGATTTCCTCAATAATCAATGCAAACCGTTTTTCATTg          >  2:4964583/1‑93 (MQ=255)
                                                                               aaCGTTACGGAAG‑CCGTTTTTTCTTCTGTCGCCATCTCTTTATTTTTGCGTTGGTTGATTTCCTCAATAATCAATGCAAACCGTTTTTCATTg          >  1:1979316/1‑93 (MQ=255)
                                                                                aCGTTCCGGAAG‑CCGTTTTTTCTTCTGTCGCCATCTCTTTATTTTTGCGTTGGTTGATTTCCTCAATAATCAATGCAAACCGTTTTTCATTGa         <  2:6703797/93‑1 (MQ=255)
                                                                                aCGTTACGGAAG‑CCGTTTTTTCTTCTGTCGCCATCTCTTTATTTTTGCGTTGGTTGATTTCCTCAATAATCAATGCAAACCGTTTTTCATTGa         >  2:618189/1‑93 (MQ=255)
                                                                                 cGTTACGGAAG‑CCGTTTTTTCTTCTGTCGCCATCTCTTTATTTTTGCGTTGGTTGATTTCCTCAATAATCAATGCAAACCGTTTTTCATTGAg        <  1:5917729/93‑1 (MQ=255)
                                                                                   ttACGGAAG‑CCGTTTTTTCTTCTGTCGCCATCTCTTTATTTTTGCGTTGGTTGATTTCCTCAATAATCAATGCAAACCGTTTTTCATTGAgtg      >  1:5999114/1‑93 (MQ=255)
                                                                                    tACGGAAG‑CCGTTTTTTCTTCTGTCGCCATCTCTTTATTTTTGCGTTGGTTGATTTCCTCAATAATCAATGCAAACCGTTTTTCATTGAgtgt     <  1:495977/93‑1 (MQ=255)
                                                                                     aCGGAAG‑CCGTTTTTTCTTCTGTCGCCATCTCTTTATTTTTGCGTTGGTTGATTTCCTCAATAATCAATGCAAACCGTTTTTCATTGAgtgtg    <  2:1104765/93‑1 (MQ=255)
                                                                                            |                                                                                        
TTTTTCATAATAAGTCCATTTTTTAAACGTAGGGAGGCCGCCACCCACCAGGATGGAAATAGCGGCTATTGACATTTATAACGTTACGGAAGCCCGTTTTTTCTTCTGTCGCCATCTCTTTATTTTTGCGTTGGTTGATTTCCTCAATAATCAATGCAAACCGTTTTTCATTGAGTGTGTA  >  NC_000913/4061867‑4062047

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output Only 200 of 209 total aligned reads displayed.
TTTTCATAATAAGTCCATTTTTTAAACGTAGGGAGGCCGCCACCCACCAGGATGGAAATAGCGGCTATTGACATTTATAACGTTACGGAAGCCCGTTTTTTCTTCTGTCGCCATCTCTTTATTTTTGCGTTGGTTGATTTCCTCAATAATCAATGCAAACCGTTTTTCATTGAGTGTGTAAAAGAAT  >  NC_000913/4061868‑4062054
                                                                                             |                                                                                             
TTTTCATAATAAGTCCATTTTTTAAACGTAGGGAGGCCGCCACCCACCAGGATGGAAATAGCGGCTATTGACATTTATAACGTTACGGAAGCCG                                                                                               >  SRR4302543.3491931/1‑94 (MQ=60)
TTTTCATAATAAGTCCATTTTTTAAACGTAGGGAGGCCGCCACCCACCAGGATGGAAATAGCGGCTATTGACATTTATAACGTTACGGAAGCCG                                                                                               <  SRR4302543.4925455/94‑1 (MQ=60)
  TTCATAATAAGTCCATTTTTTAAACGTAGGGAGGCCGCCACCCACCAGGATGGAAATAGCGGCTATTGACATTTATAACGTTACGGAAG‑CCGTT                                                                                            >  SRR4302543.4680883/1‑94 (MQ=60)
  TTCATAATAAGTCCATTTTTTAAACGTAGGGAGGCCGCCACCCACCAGGATGGAAATAGCGGCTATTGACATTTATAACGTTACGGAAG‑CCGTT                                                                                            <  SRR4302543.1137586/94‑1 (MQ=60)
   TCATAATAAGTCCATTTTTTAAACGTAGGGAGGCCGCCACCCACCAGGATGGAAATAGCGGCTATTGACATTTATAACGTTACGGAAG‑CCGTTT                                                                                           >  SRR4302543.2481811/1‑94 (MQ=60)
   TCATAATAAGTCCATTTTTTAAACGTAGGGAGGCCGCCACCCACCAGGATGGAAATAGCGGCTATTGACATTTATAACGTTACGGAAG‑CCGTTT                                                                                           <  SRR4302543.1612229/94‑1 (MQ=60)
    CATAATAAGTCCATTTTTTAAACGTAGGGAGGCCGCCACCCACCAGGATGGAAATAGCGGCTATTGACATTTATAACGTTACGGAAG‑CCGTTTT                                                                                          >  SRR4302543.6705110/1‑94 (MQ=60)
    CATAATAAGTCCATTTTTTAAACGTAGGGAGGCCGCCACCCACCAGGATGGAAATAGCGGCTATTGACATTTATAACGTTACGGAAG‑CCGTTTT                                                                                          >  SRR4302543.1708929/1‑94 (MQ=60)
    CATAATAAGTCCATTTTTTAAACGTAGGGAGGCCGCCACCCACCAGGATGGAAATAGCGGCTATTGACATTTATAACGTTACGGAAG‑CCGTTTT                                                                                          <  SRR4302543.1050618/94‑1 (MQ=60)
     ATAATAAGTCCATTTTTTAAACGTAGGGAGGCCGCCACCCACCAGGATGGAAATAGCGGCTATTGACATTTATAACGTTACGGAAG‑CCGTTTTT                                                                                         <  SRR4302543.6116318/94‑1 (MQ=60)
     ATAATAAGTCCATTTTTTAAACGTAGGGAGGCCGCCACCCACCAGGATGGAAATAGCGGCTATTGACATTTATAACGTTACGGAAG‑CCGTTTTT                                                                                         >  SRR4302543.698472/1‑94 (MQ=60)
     ATAATAAGTCCATTTTTTAAACGTAGGGAGGCCGCCACCCACCAGGATGGAAATAGCGGCTATTGACATTTATAACGTTACGGAAG‑CCGTTTTT                                                                                         >  SRR4302543.4049162/1‑94 (MQ=60)
     ATAATAAGTCCATTTTTTAAACGTAGGGAGGCCGCCACCCACCAGGATGGAAATAGCGGCTATTGACATTTATAACGTTACGGAAG‑CCGTTTTT                                                                                         >  SRR4302543.2795273/1‑94 (MQ=60)
     ATAATAAGTCCATTTTTTAAACGTAGGGAGGCCGCCACCCACCAGGATGGAAATAGCGGCTATTGACATTTATAACGTTACGGAAG‑CCGTTTTT                                                                                         >  SRR4302543.6268518/1‑94 (MQ=60)
     ATAATAAGTCCATTTTTTAAACGTAGGGAGGCCGCCACCCACCAGGATGGAAATAGCGGCTATTGACATTTATAACGTTACGGAAG‑CCGTTTTT                                                                                         >  SRR4302543.4615649/1‑94 (MQ=60)
      TAATAAGTCCATTTTTTGAACGTAGGGAGGCCGCCACCCACCAGGATGGAAATAGCGGCTATTGACATTTATAACGTTACGGAAG‑CCGTTTTTT                                                                                        <  SRR4302543.7115149/94‑1 (MQ=60)
      TAATAAGTCCATTTTTTAAACGTAGGGAGGCCGCCACCCACCAGGATGGAAATAGCGGCTATTGACATTTATAACGTTACGGAAG‑CCGTTTTTT                                                                                        >  SRR4302543.2426899/1‑94 (MQ=60)
      TAATAAGTCCATTTTTTAAACGTAGGGAGGCCGCCACCCACCAGGATGGAAATAGCGGCTATTGACATTTATAACGTTACGGAAG‑CCGTTTTTT                                                                                        <  SRR4302543.2299003/94‑1 (MQ=60)
      TAATAAGTCCATTTTTTAAACGTAGGGAGGCCGCCACCCACCAGGATGGAAATAGCGGCTATTGACATTTATAACGTTACGGAAG‑CCGTTTTTT                                                                                        >  SRR4302543.5553909/1‑94 (MQ=60)
      TAATAAGTCCATTTTTTAAACGTAGGGAGGCCGCCACCCACCAGGATGGAAATAGCGGCTATTGACATTTATAACGTTACGGAAG‑CCGTTTTTT                                                                                        >  SRR4302543.7203904/1‑94 (MQ=60)
       AATAAGTCCATTTTTTAAACGTAGGGAGGCCGCCACCCACCAGGATGGAAATAGCGGCTATTGACATTTATAACGTTACGGAAG‑CCGTTTTTTC                                                                                       >  SRR4302543.2668739/1‑94 (MQ=60)
       AATAAGTCCATTTTTTAAACGTAGGGAGGCCGCCACCCACCAGGATGGAAATAGCGGCTATTGACATTTATAACGTTACGGAAG‑CCGTTTTTTC                                                                                       <  SRR4302543.4523905/94‑1 (MQ=60)
       AATAAGTCCATTTTTTAAACGTAGGGAGGCCGCCACCCACCAGGATGGAAATAGCGGCTATTGACATTTATAACGTTACGGAAG‑CCGTTTTTTC                                                                                       >  SRR4302543.498272/1‑94 (MQ=60)
        ATAAGTCCATTTTTTAAACGTAGGGAGGCCGCCACCCACCAGGATGGAAATAGCGGCTATTGACATTTATAACGTTACGGAAG‑CCGTTTTTTCT                                                                                      >  SRR4302543.7230632/1‑94 (MQ=60)
        ATAAGTCCATTTTTTAAACGTAGGGAGGCCGCCACCCACCAGGATGGAAATAGCGGCTATTGACATTTATAACGTTACGGAAG‑CCGTTTTTTCT                                                                                      >  SRR4302543.6306172/1‑94 (MQ=60)
        ATAAGTCCATTTTTTAAACGTAGGGAGGCCGCCACCCACCAGGATGGAAATAGCGGCTATTGACATTTATAACGTTACGGAAG‑CCGTTTTTTCT                                                                                      >  SRR4302543.6449685/1‑94 (MQ=60)
        ATAAGTCCATTTTTTAAACGTAGGGAGGCCGCCACCCACCAGGATGGAAATAGCGGCTATTGACATTTATAACGTTACGGAAG‑CCGTTTTTTCT                                                                                      <  SRR4302543.6452723/94‑1 (MQ=60)
        ATAAGTCCATTTTTTAAACGTAGGGAGGCCGCCACCCACCAGGATGGAAATAGCGGCTATTGACATTTATAACGTTACGGAAG‑CCGTTTTTTCT                                                                                      <  SRR4302543.6606906/94‑1 (MQ=60)
        ATAAGTCCATTTTTTAAACGTAGGGAGGCCGCCACCCACCAGGATGGAAATAGCGGCTATTCACATTTATAACGTTACGGAAG‑CCGTTTTTTCT                                                                                      >  SRR4302543.2732139/1‑94 (MQ=60)
         TAAGTCCATTTTTTAAACGTAGGGAGGCCGCCACCCACCAGGATGGAAATAGCGGTTATTGACATTTATAACGTTACGGAAG‑CCGTTTTTTCTT                                                                                     <  SRR4302543.6469887/94‑1 (MQ=60)
         TAAGTCCATTTTTTAAACGTAGGGAGGCCGCCACCCACCAGGATGGAAATAGCGGCTATTGACATTTATAACGTTACGGAAG‑CCGTTTTTTCTT                                                                                     >  SRR4302543.1108159/1‑94 (MQ=60)
         TAAGTCCATTTTTTAAACGTAGGGAGGCCGCCACCCACCAGGATGGAAATAGCGGCTATTGACATTTATAACGTTACGGAAG‑CCGTTTTTTCTT                                                                                     >  SRR4302543.3529168/1‑94 (MQ=60)
         TAAGTCCATTTTTTAAACGTAGGGAGGCCGCCACCCACCAGGATGGAAATAGCGGCTATTGACATTTATAACGTTACGGAAG‑CCGTTTTTTCTT                                                                                     >  SRR4302543.1709478/1‑94 (MQ=60)
          AAGTCCATTTTTTAAACGTAGGGAGGCCGCCACCCACCAGGATGGAAATAGCGGCTATTGACATTTATAACGTTACGGAAG‑CCGTTTTTTCTTC                                                                                    >  SRR4302543.6723821/1‑94 (MQ=60)
          AAGTCCATTTTTTAAACGTAGGGAGGCCGCCACCCACCAGGATGGAAATAGCGGCTATTGACATTTATAACGTTACGGAAG‑CCGTTTTTTCTTC                                                                                    >  SRR4302543.2606965/1‑94 (MQ=60)
           AGTCCATTTTTTAAACGTAGGGAGGCCGCCACCCACCAGGATGGAAATAGCGGCTATTGACATTTATAACGTTACGGAAG‑CCGTTTTTTCTTCT                                                                                   <  SRR4302543.2305380/94‑1 (MQ=60)
           AGTCCATTTTTTAAACGTAGGGAGGCCGCCACCCACCAGGATGGAAATAGCGGCTATTGACATTTATAACGTTACGGAAG‑CCGTTTTTTCTTCT                                                                                   <  SRR4302543.2862405/94‑1 (MQ=60)
           AGTCCATTTTTTAAACGTAGGGAGGCCGCCACCCACCAGGATGGAAATAGCGGCTATTGACATTTATAACGTTACGGAAG‑CCGTTTTTTCTTCT                                                                                   <  SRR4302543.6749240/94‑1 (MQ=60)
           AGTCCATTTTTTAAACGTAGGGAGGCCGCCACCCACCAGGATGGAAATAGCGGCTATTGACATTTATAACGTTACGGAAG‑CCGTTTTTTCTTCT                                                                                   >  SRR4302543.7569499/1‑94 (MQ=60)
            GTCCATTTTTTAAACGTAGGGAGGCCGCCACCCACCAGGATGGAAATAGCGGCTATTGACATTTATAACGTTACGGAAG‑CCGTTTTTTCTTCTG                                                                                  <  SRR4302543.5506829/94‑1 (MQ=60)
             TCCATTTTTTAAACGTAGGGAGGCCGCCACCCACCAGGATGGAAATAGCGGCTATTGACATTTATAACGTTACGGAAG‑CCGTTTTTTCTTCTGT                                                                                 >  SRR4302543.3032991/1‑94 (MQ=60)
               CATTTTTTAAACGTAGGGAGGCCGCCACCCACCAGGATGGAAATAGCGGCTATTGACATTTATAACGTTACGGAAG‑CCGTTTTTTCTTCTGTCG                                                                               <  SRR4302543.5902217/94‑1 (MQ=60)
               CATTTTTTAAACGTAGGGAGGCCGCCACCCACCAGGATGGAAATAGCGGCTATTGACATTTATAACGTTACGGAAG‑CCGTTTTTTCTTCTGTCG                                                                               >  SRR4302543.3146803/1‑94 (MQ=60)
               CATTTTTTAAACGTAGGGAGGCCGCCACCCACCAGGATGGAAATAGCGGCTATTGACATTTATAACGTTACGGAAG‑CCGTTTTTTCTTCTGTCG                                                                               >  SRR4302543.4919229/1‑94 (MQ=60)
                ATTTTTTAAACGTAGGGAGGCCGCCACCCACCAGGATGGAAATAGCGGCTATTGACATTTATAACGTTACGGAAG‑CCGTTTTTTCTTCTGTCGC                                                                              <  SRR4302543.3146457/94‑1 (MQ=60)
                ATTTTTTAAACGTAGGGAGGCCGCCACCCACCAGGATGGAAATAGCGGCTATTGACATTTATAACGTTACGGAAG‑CCGTTTTTTCTTCTGTCGC                                                                              >  SRR4302543.4570733/1‑94 (MQ=60)
                 TTTTTTAAACGTAGGGAGGCCGCCACCCACCAGGATGGAAATAGCGGCTATTGACATTTATAACGTTACGGAAG‑CCGTTTTTTCTTCTGTCGCC                                                                             <  SRR4302543.7970929/94‑1 (MQ=60)
                  TTTTTAAACGTAGGGAGGCCGCCACCCACCAGGATGGAAATAGCGGCTATTGACATTTATAACGTTACGGAAG‑CCGTTTTTTCTTCTGTCGCCA                                                                            >  SRR4302543.5364729/1‑94 (MQ=60)
                  TTTTTAAACGTAGGGAGGCCGCCACCCACCAGGATGGAAATAGCGGCTATTGACATTTATAACGTTACGGAAG‑CCGTTTTTTCTTCTGTCGCCA                                                                            <  SRR4302543.5411509/94‑1 (MQ=60)
                  TTTTTAAACGTAGGGAGGCCG‑CACCCCCCCGGGTTGGAATAGCGGCTTTTGACATTTATAACGTTACGGAAGCCCGTTTTTTtttctttggcat                                                                            >  SRR4302543.2980569/1‑82 (MQ=49)
                   TTTTAAACGTAGGGAGGCCGCCACCCACCAGGATGGAAATAGCGGCTATTGACATTTATAACGTTACGGAAG‑CCGTTTTTTCTTCTGTCGCCAT                                                                           >  SRR4302543.4960483/1‑94 (MQ=60)
                     TTAAACGTAGGGAGGCCGCCACCCACCAGGATGGAAATAGCGGCTATTGACATTTATAACGTTACGGAAG‑CCGTTTTTTCTTCTGTCGCCATCT                                                                         <  SRR4302543.616691/94‑1 (MQ=60)
                       AAACGTAGGGAGGCCGCCACCCACCAGGATGGAAATAGCGGCTATTGACATTTATAACGTTACGGAAG‑CCGTTTTTTCTTCTGTCGCCATCTCT                                                                       <  SRR4302543.6251092/94‑1 (MQ=60)
                       AAACGTAGGGAGGCCGCCACCCACCAGGATGGAAATAGCGGCTATTGACATTTATAACGTTACGGAAG‑CCGTTTTTTCTTCTGTCGCCATCTCT                                                                       <  SRR4302543.1049160/94‑1 (MQ=60)
                       AAACGTAGGGAGGCCGCCACCCACCAGGATGGAAATAGCGGCTATTGACATTTATAACGTTACGGAAG‑CCGTTTTTTCTTCTGTCGCCATCTCT                                                                       <  SRR4302543.1049159/94‑1 (MQ=60)
                        AACGTAGGGAGGCCGCCACCCACCAGGATGGAAATAGCGGCTATTGACATTTATAACGTTACGGAAG‑CCGTTTTTTCTTCTGTCGCCATCTCTT                                                                      <  SRR4302543.6958192/94‑1 (MQ=60)
                         ACGTAGGGAGGCCGCCACCCACCAGGATGGAAATAGCGGCTATTGACATTTATAACGTTACGGAAG‑CCGTTTTTTCTTCTGTCGCCATCTCTTT                                                                     >  SRR4302543.3716925/1‑94 (MQ=60)
                         ACGTAGGGAGGCCGCCACCCACCAGGATGGAAATAGCGGCTATTGACATTTATAACGTTACGGAAG‑CCGTTTTTTCTTCTGTCGCCATCTCTTT                                                                     >  SRR4302543.3452476/1‑94 (MQ=60)
                         ACGTAGGGAGGCCGCCACCCACCAGGATGGAAATAGCGGCTATTGACATTTATAACGTTACGGAAG‑CCGTTTTTTCTTCTGTCGCCATCTCTTT                                                                     >  SRR4302543.3452477/1‑94 (MQ=60)
                          NGNANGNNGNCCGCCACNCACNNGGNTGGAAATAGCGGCTATTGACATTTANAACGTTACGGAAG‑CCGTTTTTTCTTCTGTCGCCATCTCTTTA                                                                    <  SRR4302543.4426093/94‑1 (MQ=60)
                          CGTAGGGAGGCCGCCACCCACCAGGATGGAAATAGCGGCTATTGACATTTATAACGTTACGGGAG‑CCGTTTTTTCTTCTGTCGCCATCcttttt                                                                    >  SRR4302543.5680581/1‑88 (MQ=60)
                          CGTAGGGAGGCCGCCACCCACCAGGATGGAAATAGCGGCTATTGACATTTATAACGTTACGGAAG‑CCGTTTTTTCTTCTGTCGCCATCTCTTTA                                                                    >  SRR4302543.4807621/1‑94 (MQ=60)
                           GTAGGGAGGCCGCCACCCACCAGGATGGAAATAGCGGCTATTGACATTTATAACGTTACGGAAG‑CCGTTTTTTCTTCTGTCGCCATCTCTTTAT                                                                   >  SRR4302543.4376463/1‑94 (MQ=60)
                           GTAGGGAGGCCGCCACCCACCAGGATGGAAATAGCGGCTATTGACATTTATAACGTTACGGAAG‑CCGTTTTTTCTTCTGTCGCCATCTCTTTAT                                                                   >  SRR4302543.5067968/1‑94 (MQ=60)
                           GTAGGGAGGCCGCCACCCACCAGGATGGAAATAGCGGCTATTGACATTTATAACGTTACGGAAG‑CCGTTTTTTCTTCTGTCGCCATCTCTTTAT                                                                   >  SRR4302543.6885149/1‑94 (MQ=60)
                           GTAGGGAGGCCGCAACCCACCAGGATGGAAATAGCGGCTATTGACATTTATAACGTTACGGAAG‑CCGTTTTTTCTTCTGTCGCCATCTCTTTCT                                                                   >  SRR4302543.499183/1‑94 (MQ=60)
                            TAGGGAGGCCGCCACCCACCAGGATGGAAATAGCGGCTATTGACATTTATAACGTTACGGAAG‑CCGTTTTTTCTTCTGTCGCCATCTCTTTATT                                                                  <  SRR4302543.7741561/94‑1 (MQ=60)
                            TAGGGAGGCCGCCACCCACCAGGATGGAAATAGCGGCTATTGACATTTATAACGTTACGGAAG‑CCGTTTTTTCTTCTGTCGCCATCTCTTTATT                                                                  >  SRR4302543.6658310/1‑94 (MQ=60)
                             AGGGAGGCCGCCACCCACCAGGATGGAAATAGCGGCTATTGACATTTATAACGTTACGGAAG‑CCGTTTTTTCTTCTGTCGCCATCTCTTTATTT                                                                 <  SRR4302543.3984516/94‑1 (MQ=60)
                             AGGGAGGCCGCCACCCACCAGGATGGAAATAGCGGCTATTGACATTTATAACGTTACGGAAG‑CCGTTTTTTCTTCTGTCGCCATCTCTTTATTT                                                                 >  SRR4302543.3959480/1‑94 (MQ=60)
                              GGGAGGCCGCCACCCACCAGGATGGAAATAGCGGCTATTGACATTTATAACGTTACGGAAG‑CCGTTTTTTCTTCTGTCGCCATCTCTTTATTTT                                                                >  SRR4302543.2169719/1‑94 (MQ=60)
                              GGGAGGCCGCCACCCACCAGGATGGAAATAGCGGCTATTGACATTTATAACGTTACGGAAG‑CCGTTTTTTCTTCTGTCGCCATCTCTTTATTTT                                                                <  SRR4302543.1944736/94‑1 (MQ=60)
                               GGAGGCCGCCACCCACCAGGATGGAAATAGCGGCTATTGACATTTATAACGTTACGGAAG‑CCGTTTTTTCTTCTGTCGCCATCTCTTTATTTTT                                                               <  SRR4302543.7478655/94‑1 (MQ=60)
                                 AGGCCGCCACCCACCAGGATGGAAATAGCGGCTATTGACATTTATAACGTTACGGAAG‑CCGTTTTTTCTTCTGTCGCCATCTCTTTATTTTTGC                                                             >  SRR4302543.234145/1‑94 (MQ=60)
                                  GGCCGCCACCCACCAGGATGGAAATAGCGGCTATTGACATTTATAACGTTACGGAAG‑CCGTTTTTTCTTCTGTCGCCATCTCTTTATTTTTGCG                                                            >  SRR4302543.5296675/1‑94 (MQ=60)
                                   GCCGCCACCCACCAGGATGGAAATAGCGGCTATTGACATTTATAACGTTACGGAAG‑CCGTTTTTTCTTCTGTCGCCATCTCTTTATTTTTGCGT                                                           <  SRR4302543.6454704/94‑1 (MQ=60)
                                   GCCGCCACCCACCAGGATGGAAATAGCGGCTATTGACATTTATAACGTTACGGAAG‑CCGCTTTTTCTTCTGTCGCCATCTCTTTATTTTTGCGT                                                           >  SRR4302543.5517021/1‑94 (MQ=60)
                                    CCGCCACCCACCAGGATGGAAATAGCGGCTATTGACATTTATAACGTTACGGAAG‑CCGTTTTTTCTTCTGTCGCCATCTCTTTATTTTTGCGTT                                                          <  SRR4302543.7486898/94‑1 (MQ=60)
                                    CCGCCACCCACCAGGATGGAAATAGCGGCTATTGACATTTATAACGTTACGGAAG‑CCGTTTTTTCTTCTGTCGCCATCTCTTTATTTTTGCGTT                                                          <  SRR4302543.619136/94‑1 (MQ=60)
                                     CGCCACCCACCAGGATGGAAATAGCGGCTATTGACATTTATAACGTTACGGAAG‑CCGTTTTTTCTTCTGTCGCCATCTCTTTATTTTTGCGTTG                                                         <  SRR4302543.6711685/94‑1 (MQ=60)
                                     CGCCACCCACCAGGATGGAAATAGCGGCTATTGACATTTATAACGTTACGGAAG‑CCGTTTTTTCTTCTGTCGCCATCTCTTTATTTTTGCGTTG                                                         >  SRR4302543.553285/1‑94 (MQ=60)
                                     CGCCACCCACCAGGATGGAAATAGCGGCTATTGACATTTATAACGTTACGGAAG‑CCGTTTTTTCTTCTGTCGCCATCTCTTTATTTTTGCGTTG                                                         <  SRR4302543.3026717/94‑1 (MQ=60)
                                     CGCCACCCACCAGGATGGAAATAGCGGCTATTGACATTTATAACGTTACGGAAG‑CCGTTTTTTCTTCTGTCGCCATCTCTTTATTTTTGCGTTG                                                         >  SRR4302543.5377390/1‑94 (MQ=60)
                                     CGCCACCCACCAGGATGGAAATAGCGGCTATTGACATTTATAACGTTACGGAAG‑CCGTTTTTTCTTCTGTCGCCATCTCTTTATTTTTGCGTTG                                                         >  SRR4302543.5349716/1‑94 (MQ=60)
                                      GCCACCCACCAGGATGGAAATAGCGGCTATTGACATTTATAACGTTACGGAAG‑CCGTTTTTTCTTCTGTCGCCATCTCTTTATTTTTGCGTTGG                                                        >  SRR4302543.2668735/1‑94 (MQ=60)
                                       CCGCCCACCAGGATGGAAATAGCGGCTATTGACATTTATAACGTTACGGAAG‑CCGTTTTTTCTTCTGTCGCCATCTCTTTATTTTTGCGTTGGT                                                       <  SRR4302543.4303114/94‑1 (MQ=60)
                                       CCACCCACCAGGATGGAAATAGCGGCTATTGACATTTATAACGTTACGGAAG‑CCGTTTTTTCTTCTGTCGCCATCTCTTTATTTTTGCGTTGGT                                                       <  SRR4302543.2465465/94‑1 (MQ=60)
                                       CCACCCACCAGGATGGAAATAGCGGCTATTGACATTTATAACGTTACGGAAG‑CCGTTTTTTCTTCTGTCGCCATCTCTTTATTTTTGCGTTGGT                                                       >  SRR4302543.286885/1‑94 (MQ=60)
                                       CCACCCACCAGGATGGAAATAGCGGCTATTGACATTTATAACGTTACGGAAG‑CCGTTTTTTCTTCTGTCGCCATCTCTTTATTTTTGCGTTGGT                                                       <  SRR4302543.4693355/94‑1 (MQ=60)
                                       CCACCCACCAGGATGGAAATAGCGGCTATTGACATTTATAACGTTACGGAAG‑CCGTTTTTTCTTCTGTCGCCATCTCTTTATTTTTGCGTTGGT                                                       <  SRR4302543.3667789/94‑1 (MQ=60)
                                       CCACCCACCAGGATGGAAATAGCGGCTATTGACATTTATAACGTTACGGAAG‑CCGTTTTTTCTTCTGTCGCCATCTCTTTATTTTTGCGTTGGT                                                       <  SRR4302543.2462296/94‑1 (MQ=60)
                                       CCACCCACCAGGATGGAAATAGCGGCTATTGACATTTATAACGTTACGGAAG‑CAGTTTTTTCTTCTGTCGCCATCTCTTTCTTTTCGCGTTGGT                                                       >  SRR4302543.286886/1‑94 (MQ=60)
                                        CACCCACCAGGATGGAAATAGCGGCTATTGACATTTATAACGTTACGGAAG‑CCGTTTTTTCTTCTGTCGCCATCTCTTTATTTTTGCGTTGGTT                                                      >  SRR4302543.4271781/1‑94 (MQ=60)
                                        CACCCACCAGGATGGAAATAGCGGCTATTGACATTTATAACGTTACGGAAG‑CCGTTTTTTCTTCTGTCGCCATCTCTTTATTTTTGCGTTGGTT                                                      >  SRR4302543.1811452/1‑94 (MQ=60)
                                         ACCCACCAGGATGGAAATAGCGGCTATTGACATTTATAACGTTACGGAAG‑CCGTTTTTTCTTCTGTCGCCATCTCTTTATTTTTGCGTTGGTTG                                                     >  SRR4302543.8009722/1‑94 (MQ=60)
                                             ACCAGGATGGAAATAGCGGCTATTGACATTTATAACGTTACGGAAG‑CCGTTTTTTCTTCTGTCGCCATCTCTTTATTTTTGCGTTGGTTGATTT                                                 >  SRR4302543.553129/1‑94 (MQ=60)
                                             ACCAGGATGGAAATAGCGGCTATTGACATTTATAACGTTACGGAAG‑CCGTTTTTTCTTCTGTCGCCATCTCTTTATTTTTGCGTTGGTTGATTT                                                 >  SRR4302543.5953374/1‑94 (MQ=60)
                                             ACCAGGATGGAAATAGCGGCTATTGACATTTATAACGTTACGGAAG‑CCGTTTTTTCTTCTGTCGCCATCTCTTTATTTTTGCGTTGGTTGATTT                                                 >  SRR4302543.3326846/1‑94 (MQ=60)
                                             ACCAGGATGGAAATAGCGGCTATTGACATTTATAACGTTACGGAAG‑CCGTTTTTTCTTCTGTCGCCATCTCTTTATTTTTGCGTTGGTTGATTT                                                 <  SRR4302543.3903830/94‑1 (MQ=60)
                                              CCAGGATGGAAATAGCGGCTATTGACATTTATAACGTTACGGAAG‑CCGTTTTTTCTTCTGTCGCCATCTCTTTATTTTTGCGTTGGTTGATTTC                                                >  SRR4302543.4491638/1‑94 (MQ=60)
                                               CAGGATGGAAATAGCGGCTATTGACATTTATAACGTTACGGAAG‑CCGTTTTTTCTTCTGTCGCCATCTCTTTATTTTTGCGTTGGTTGATTTCC                                               >  SRR4302543.6240439/1‑94 (MQ=60)
                                                AGGATGGAAATAGCGGCTATTGACATTTATAACGTTACGGAAG‑CCGTTTTTTCTTCTGTCGCCATCTCTTTATTTTTGCGTTGGTTGATTTCCT                                              <  SRR4302543.4356200/94‑1 (MQ=60)
                                                AGGATGGAAATAGCGGCTATTGACATTTATAACGTTACGGAAG‑CCGTTTTTTCTTCTGTCGCCATCTCTTTATTTTTGCGTTGGTTGATTTCCT                                              <  SRR4302543.2502942/94‑1 (MQ=60)
                                                AGGATGGAAATAGCGGCTATTGACATTTATAACGTTACGGAAG‑CCGTTTTTTCTTCTGTCGCCATCTCTTTATTTTTGCGTTGGTTGATTTCCT                                              >  SRR4302543.3763061/1‑94 (MQ=60)
                                                 GGATGGAAATAGCGGCTATTGACATTTATAACGTTACGGAAG‑CCGTTTTTTCTTCTGTCGCCATCTCTTTATTTTTGCGTTGGTTGATTTCCTC                                             <  SRR4302543.813463/94‑1 (MQ=60)
                                                 GGATGGAAATAGCGGCTATTGACATTTATAACGTTACGGAAG‑CCGTTTTTTCTTCTGTCGCCATCTCTTTATTTTTGCGTTGGTTGATTTCCTC                                             >  SRR4302543.3897026/1‑94 (MQ=60)
                                                 GGATGGAAATAGCGGCTATTGACATTTATAACGTTACGGAAG‑CCGTTTTTTCTTCTGTCGCCATCTCTTTATTTTTGCGTTGGTTGATTTCCTC                                             >  SRR4302543.2726677/1‑94 (MQ=60)
                                                  GATGGAAATAGCGGCTATTGACATTTATAACGTTACGGAAG‑CCGTTTTTTCTTCTGTCGCCATCTCTTTATTTTTGCGTTGGTTGATTTCCTCA                                            >  SRR4302543.4220292/1‑94 (MQ=60)
                                                  GATGGAAATAGCGGCTATTGACATTTATAACGTTACGGAAG‑CCGTTTTTTCTTCTGTCGCCATCTCTTTATTTTTGCGTTGGTTGATTTCCTCA                                            >  SRR4302543.4278698/1‑94 (MQ=60)
                                                   ATGGAAATAGCGGCTATTGACATTTATAACGTTACGGAAG‑CCGTTTTTTCTTCTGTCGCCATCTCTTTATTTTTGCGTTGGTTGATTTCCTCAA                                           <  SRR4302543.825125/94‑1 (MQ=60)
                                                   ATGGAAATAGCGGCTATTGACATTTATAACGTTACGGAAG‑CCGTTTTTTCTTCTGTCGCCATCTCTTTATTTTTGCGTTGGTTGATTTCCTCAA                                           >  SRR4302543.672412/1‑94 (MQ=60)
                                                    TGGAAATAGCGGCTATTGACATTTATAACGTTACGGAAG‑CCGTTTTTTCTTCTGTCGCCATCTCTTTATTTTTGCGTTGGTTGATTTCCTCAAT                                          <  SRR4302543.295043/94‑1 (MQ=60)
                                                    TGGAAATAGCGGCTATTGACATTTATAACGTTACGGAAG‑CCGTTTTTTCTTCTGTCGCCATCTCTTTATTTTTGCGTTAGTTGATTTCCTCAAT                                          <  SRR4302543.5113525/94‑1 (MQ=60)
                                                      GAAATAGCGGCTATTGACATTTATAACGTTACGGAAG‑CCGTTTTTTCTTCTGTCGCCATCTCTTTATTTTTGCGTTGGTTGATTTCCTCAATAA                                        >  SRR4302543.2578233/1‑94 (MQ=60)
                                                      GAAATAGCGGCTATTGACATTTATAACGTTACGGAAG‑CCGTTTTTTCTTCTGTCGCCATCTCTTTATTTTTGCGTTGGTTGATTTCCTCAATAA                                        <  SRR4302543.5313316/94‑1 (MQ=60)
                                                      GAAATAGCGGCTATTGACATTTATAACGGTACGGAAG‑CCGTTTTTTCTTCTGTCGCCATCTCTTTATTTTTGCGTTGGTTGATTTCCTCAATAA                                        <  SRR4302543.5313317/94‑1 (MQ=60)
                                                       AAATAGCGGCTATTGACATTTATAACGTTACGGAAG‑CCGTTTTTTCTTCTGTCGCCATCTCTTTATTTTTGCGTTGGTTGATTTCCTCAATAAT                                       >  SRR4302543.2459751/1‑94 (MQ=60)
                                                        AATAGCGGCTATTGACATTTATAACGTTACGGAAG‑CCGTTTTTTCTTCTGTCGCCATCTCTTTATTTTTGCGTTGGTTGATTTCCTCAATAATC                                      >  SRR4302543.2807977/1‑94 (MQ=60)
                                                        AATAGCGGCTATTGACATTTATAACGTTACGGAAG‑CCGTTTTTTCTTCTGTCGCCATCTCTTTATTTTTGCGTTGGTTGATTTCCTCAATAATC                                      >  SRR4302543.6612492/1‑94 (MQ=60)
                                                        AATAGCGGCTATTGACATTTATAACGTTACGGAAG‑CCGTTTTTTCTTCTGTCGCCATCTCTTTATTTTTGCGTTGGTTGATTTCCTCAATAATC                                      >  SRR4302543.2807749/1‑94 (MQ=60)
                                                         ATAGCGGCTATTGACATTTATAACGTTACGGAAG‑CCGTTTTTTCTTCTGTCGCCATCTCTTTATTTTTGCGTTGGTTGATTTCCTCAATAATCA                                     >  SRR4302543.545184/1‑94 (MQ=60)
                                                         ATAGCGGCTATTGACATTTATAACGTTACGGAAG‑CCGTTTTTTCTTCTGTCGCCATCTCTTTATTTTTGCGTTGGTTGATTTACTCAATAATCA                                     >  SRR4302543.545183/1‑94 (MQ=60)
                                                           AGCGGCTATTGACATTTATAACGTTACGGAAG‑CCGTTTTTTCTTCTGTCGCCATCTCTTTATTTTTGCGTTGGTTGATTTCCTCAATAATCAAT                                   <  SRR4302543.1279831/94‑1 (MQ=60)
                                                           AGCGGCTATTGACATTTATAACGTTACGGAAG‑CCGTTTTTTCTTCTGTCGCCATCTCTTTATTTTTGCGTTGGTTGATTTCCTCAATAATCAAT                                   >  SRR4302543.2870391/1‑94 (MQ=60)
                                                           AGCGGCTATTGACATTTATAACGTTACGGAAG‑CCGTTTTTTCTTCTGTCGCCATCTCTTTATTTTTGCGTTGGTTGATTTCCTCAATAATCAAT                                   >  SRR4302543.3799498/1‑94 (MQ=60)
                                                           AGCGGCTATTGACATTTATAACGTTACGGAAG‑CCGTTTTTTCTTCTGTCGCCATCTCTTTATTTTTGCGTTGGTTGATTTCCTCAATAATCAAT                                   <  SRR4302543.736166/94‑1 (MQ=60)
                                                            GCGGCTATTGACATTTATAACGTTACGGAAG‑CCGTTTTTTCTTCTGTCGCCATCTCTTTATTTTTGCGTTGGTTGATTTCCTCAATAATCAATG                                  <  SRR4302543.888518/94‑1 (MQ=60)
                                                              GGCTATTGACATTTATAACGTTACGGAAG‑CCGTTTTTTCTTCTGTCGCCATCTCTTTATTTTTGCGTTGGTTGATTTCCTCAATAATCAATGCA                                >  SRR4302543.4263074/1‑94 (MQ=60)
                                                              GGCTATTGACATTTATAACGTTACGGAAG‑CCGTTTTTTCTTCTGTCGCCATCTCTTTATTTTTGCGTTGGTTGATTTCCTCAATAATCAATGCA                                <  SRR4302543.3611295/94‑1 (MQ=60)
                                                              GGCTATTGACATTTATAACGTTACGGAAG‑CCGTTTTTTCTTCTGTCGCCATCTCTTTATTTTTGCGTTGGTTGATTTCCTCAATAATCAATGCA                                >  SRR4302543.6135703/1‑94 (MQ=60)
                                                                CTATTGACATTTATAACGTTACGGAAG‑CCGTTTTTTCTTCTGTCGCCATCTCTTTATTTTTGCGTTGGTTGATTTCCTCAATAATCAATGCAAA                              >  SRR4302543.1449362/1‑94 (MQ=60)
                                                                CTATTGACATTTATAACGTTACGGAAG‑CCGTTTTTTCTTCTGTCGCCATCTCTTTATTTTTGCGTTGGTTGATTTCCTCAATAATCAATGCAAA                              >  SRR4302543.3448911/1‑94 (MQ=60)
                                                                CTATTGACATTTATAACGTTACGGAAG‑CCGTTTTTTCTTCTGTCGCCATCTCTTTATTTTTGCGTTGGTTGATTTCCTCAATAATCAATGCAAA                              >  SRR4302543.3881853/1‑94 (MQ=60)
                                                                CTATTGACATTTATAACGTTACGGAAG‑CCGTTTTTTCTTCTGTCGCCATCTCTTTATTTTTGCGTTGGTTGATTTCCTCAATAATCAATGCAAA                              <  SRR4302543.3652013/94‑1 (MQ=60)
                                                                 TATTGACATTTATAACGTTACGGAAG‑CCGTTTTTTCTTCTGTCGCCATCTCTTTATTTTTGCGTTGGTTGATTTCCTCAATAATCAATGCAAAC                             >  SRR4302543.6502540/1‑94 (MQ=60)
                                                                 TATTGACATTTATAACGTTACGGAAG‑CCGTTTTTTCTTCTGTCGCCATCTCTTTATTTTTGCGTTGGTTGATTTCCTCAATAATCAATGCAAAC                             >  SRR4302543.6367613/1‑94 (MQ=60)
                                                                  ATTGACATTTATAACGTTACGGAAG‑CCGTTTTTTCTTCTGTCGCCATCTCTTTATTTTTGCGTTGGTTGATTTCCTCAATAATCAATGCAAACC                            >  SRR4302543.166360/1‑94 (MQ=60)
                                                                  ATTGACATTTATAACGTTACGGAAG‑CCGTTTTTTCTTCTGTCGCCATCTCTTTATTTTTGCGTTGGTTGATTTCCCCAATAATCAATGC‑AACCG                           >  SRR4302543.4595322/1‑94 (MQ=60)
                                                                   TTGACATTTATAACGTTACGGAAG‑CCGTTTTTTCTTCTGTCGCCCTCTCTTTATTTTTGCGTTGGTTGATTTCCTCAATAATCAATGCAAACCG                           >  SRR4302543.4950795/1‑94 (MQ=60)
                                                                   TTGACATTTATAACGTTACGGAAG‑CCGTTTTTTCTTCTGTCGCCATCTCTTTATTTTTGCGTTGGTTGATTTCCTCAATAATCAATGCAAACCG                           >  SRR4302543.5434138/1‑94 (MQ=60)
                                                                   TTGACATTTATAACGTTACGGAAG‑CCGTTTTTTCTTCTGTCGCCATCTCTTTATTTTTGCGTTGGTTGATTTCCTCAATAATCAATGCAAACCG                           <  SRR4302543.5383359/94‑1 (MQ=60)
                                                                   TTGACATTTATAACGTTACGGAAG‑CCGTTTTTTCTTCTGTCGCCATCTCTTTATTTTTGCGTTGGTTGATTTCCTCAATAATCAATGCAAACCG                           <  SRR4302543.6945297/94‑1 (MQ=60)
                                                                    TGACATTTATAACGTTACGGAAG‑CCGTTTTTTCTTCTGTCGCCATCTCTTTATTTTTGCGTTGGTTGATTTCCTCAATAATCAATGCAAACCGT                          <  SRR4302543.170267/94‑1 (MQ=60)
                                                                    TGACATTTATAACGTTACGGAAG‑CCGTTTTTTCTTCTGTCGCCATCTCTTTATTTTTGCGTTGGTTGATTTCCTCAATAATCAATGCAAACCGT                          >  SRR4302543.4767294/1‑94 (MQ=60)
                                                                    TGACATTTATAACGTTACGGAAG‑CCGTTTTTTCTTCTGTCGCCATCTCTTTATTTTTGCGTTGGTTGATTTCCTCAATAATCAATGCAAACCGT                          >  SRR4302543.87364/1‑94 (MQ=60)
                                                                     GACATTTATAACGTTACGGAAG‑CCGTTTTTTCTTCTGTCGCCATCTCTTTATTTTTGCGTTGGTTGATTTCCTCAATAATCAATGCAAACCGTT                         <  SRR4302543.1833884/94‑1 (MQ=60)
                                                                     GACATTTATAACGTTACGGAAG‑CCGTTTTTTCTTCTGTCGCCATCTCTTTATTTTTGCGTTGGTTGATTTCCTCAATAATCAATGCAAACCGTT                         >  SRR4302543.4267658/1‑94 (MQ=60)
                                                                     GACATTTATAACGTTACGGAAG‑CCGTTTTTTCTTCTGTCGCCATCTCTTTATTTTTGCGTTGGTTGATTTCCTCAATAATCAATGCAAACCGTT                         >  SRR4302543.2678189/1‑94 (MQ=60)
                                                                     GACATTTATAACGTTACGGAAG‑CCGTTTTTTCTTCTGTCGCCATCTCTTTATTTTTGCGTTGGTTGATTTCCTCAATAATCAATGCAAACCGTT                         <  SRR4302543.2937698/94‑1 (MQ=60)
                                                                      ACATTTATAACGTTACGGAAG‑CCGTTTTTTCTTCTGTCGCCATCTCTTTATTTTTGCGTTGGTTGATTTCCTCAATAATCAATGCAAACCGTTT                        >  SRR4302543.2067274/1‑94 (MQ=60)
                                                                      ACATTTATAACGTTACGGAAG‑CCGTTTTTTCTTCTGTCGCCATCTCTTTATTTTTGCGTTGGTTGATTTCCTCAATAATCAATGCAAACCGTTT                        >  SRR4302543.3909901/1‑94 (MQ=60)
                                                                       CATTTATAACGTTACGGAAG‑CCGTTTTTTCTTCTGTCGCCATCTCTTTATTTTTGCGTTGGTTGATTTCCTCAATAATCAATGCAAACCGTTTT                       >  SRR4302543.4734776/1‑94 (MQ=60)
                                                                       CATTTATAACGTTACGGAAG‑CCGTTTTTTCTTCTGTCGCCATCTCTTTATTTTTGCGTTGGGTGATTTCCTCAATAATCAATGCAAACCGTTTT                       >  SRR4302543.3519287/1‑94 (MQ=60)
                                                                        ATTTATAACGTTACGGAAG‑CCGTTTTTTCTTCTGTCGCCATCTCTTTATTTTTGCGTTGGTTGATTTCCTCAATAATCAATGCAAACCGTTTTT                      <  SRR4302543.3510865/94‑1 (MQ=60)
                                                                        ATTTATAACGTTACGGAAG‑CCGTTTTTTCTTCTGTCGCCATCTCTTTATTTTTGCGTTGGTTGATTTCCTCAATAATCAATGCAAACCGTTTTT                      <  SRR4302543.4890234/94‑1 (MQ=60)
                                                                         TTTATAACGTTACGGAAG‑CCGTTTTTTCTTCTGTCGCCATCTCTTTATTTTTGCGTTGGTTGATTTCCTCAATAATCAATGCAAACCGTTTTTC                     <  SRR4302543.924437/94‑1 (MQ=60)
                                                                         TTTATAACGTTACGGAAG‑CCGTTTTTTCTTCTGTCGCCATCTCTTTATTTTTGCGTTGGTTGATTTCCTCAATAATCAATGCAAACCGTTTTTC                     >  SRR4302543.4633793/1‑94 (MQ=60)
                                                                         TTTATAACGTTACGGAAG‑CCGTTTTTTCTTCTGTCGCCATCTCTTTATTTTTGCGTTGGTTGATTTCCTCAATAATCAATGCAAACCGTTTTTC                     <  SRR4302543.3377818/94‑1 (MQ=60)
                                                                           TATAACGTTACGGAAG‑CCGTTTTTTCTTCTGTCGCCATCTCTTTATTTTTGCGTTGGTTGATTTCCTCAATAATCAATGCAAACCGTTTTTCAT                   <  SRR4302543.831226/94‑1 (MQ=60)
                                                                           TATAACGTTACGGAAG‑CCGTTTTTTCTTCTGTCGCCATCTCTTTATTTTTGCGTTGGTTGATTTCCTCAATAATCAATGCAAACCGTTTTTCAT                   <  SRR4302543.1370446/94‑1 (MQ=60)
                                                                           TATAACGTTACGGAAG‑CCGTTTTTTCTTCTGTCGCCATCTCTTTATTTTTGCGTTGGTTGATTTCCTCAATAATCAATGCAAACCGTTTTTCAT                   >  SRR4302543.4072900/1‑94 (MQ=60)
                                                                            ATAACGTTACGGAAG‑CCGTTTTTTCTTCTGTCGCCATCTCTTTATTTTTGCGTTGGTTGATTTCCTCAATAATCAATGCAAACCGTTTTTCATT                  >  SRR4302543.3923809/1‑94 (MQ=60)
                                                                            ATAACGTTACGGAAG‑CCGTTTTTTCTTCTGTCGCCATCTCTTTATTTTTGCGTTGGTTGATTTCCTCAATAATCAATGCAAACCGTTTTTCATT                  >  SRR4302543.5998767/1‑94 (MQ=60)
                                                                             TAACGTTACGGAAG‑CCGTTTTTTCTTCTGTCGCCATCTCTTTATTTTTGCGTTGGTTGATTTCCTCAATAATCAATGCAAACCGTTTTTCATTG                 <  SRR4302543.1580541/94‑1 (MQ=60)
                                                                             TAACGTTACGGAAG‑CCGTTTTTTCTTCTGTCGCCATCTCTTTATTTTTGCGTTGGTTGATTTCCTCAATAATCAATGCAAACCGTTTTTCATTG                 >  SRR4302543.1997056/1‑94 (MQ=60)
                                                                             TAACGTTACGGAAG‑CCGTTTTTTCTTCTGTCGCCATCTCTTTATTTTTGCGTTGGTTGATTTCCTCAATAATCAATGCAAACCGTTTTTCATTG                 <  SRR4302543.7127391/94‑1 (MQ=60)
                                                                              AACGTTACGGAAG‑CCGTTTTTTCTTCTGTCGCCATCTCTTTATTTTTGCGTTGGTTGATTTCCTCAATAATCAATGCAAACCGTTTTTCATTGA                <  SRR4302543.5751819/94‑1 (MQ=60)
                                                                              AACGTTACGGAAG‑CCGTTTTTTCTTCTGTCGCCATCTCTTTATTTTTGCGTTGGTTGATTTCCTCAATAATCAATGCAAACCGTTTTTCATTGA                >  SRR4302543.7433814/1‑94 (MQ=60)
                                                                               ACGTTACGGAAG‑CCGTTTTTTCTTCTGTCGCCATCTCTTTATTTTTGCGTTGGTTGATTTCCTCAATAATCAATGCAAACCGTTTTTCATTGAG               <  SRR4302543.7844054/94‑1 (MQ=60)
                                                                               ACGTTACGGAAG‑CCGTTTTTTCTTCTGTCGCCATCTCTTTATTTTTGCGTTGGTTGATTTCCTCAATAATCAATGCAAACCGTTTTTCATTGAG               <  SRR4302543.7884396/94‑1 (MQ=60)
                                                                                CGTTACGGAAG‑CCGTTTTTTCTTCTGTCGCCATCTCTTTATTTTTGCGTTGGTTGATTTCCTCAATAATCAATGCAAACCGTTTTTCATTGAGT              <  SRR4302543.5971786/94‑1 (MQ=60)
                                                                                CGTTACGGAAG‑CCGTTTTTTCTTCTGTCGCCATCTCTTTATTTTTGCGTTGGTTGATTTCCTCAATAATCAATGCAAACCGTTTTTCATTGAGT              >  SRR4302543.1503608/1‑94 (MQ=60)
                                                                                CGTTACGGAAG‑CCGTTTTTTCTTCTGTCGCCATCTCTTTATTTTTGCGTTGGTTGATTTCCTCAATAATCAATGCAAACCGTTTTTCATTGAGT              <  SRR4302543.2754077/94‑1 (MQ=60)
                                                                                 GTTACGGAAG‑CCGTTTTTTCTTCTGTCGCCATCTCTTTATTTTTGCGTTGGTTGATTTCCTCAATAATCAATGCAAACCGTTTTTCATTGAGTG             <  SRR4302543.3414732/94‑1 (MQ=60)
                                                                                 GTTACGGAAG‑CCGTTTTTTCTTCTGTCGCCATCTCTTTATTTTTGCGTTGGTTGATTTCCTCAATAATCAATGCAAACCGTTTTTCATTGAGTG             >  SRR4302543.6078713/1‑94 (MQ=60)
                                                                                 GTTACGGAAG‑CCGTTTTTTCTTCTGTCGCCATCTCTTTATTTTTGCGTTGGTTGATTTCCTCAATAATCAATGCAAACCGTTTTTCATTGAGTG             >  SRR4302543.6054195/1‑94 (MQ=60)
                                                                                 GTTACGGAAG‑CCGTTTTTTCTTCTGTCGCCATCTCTTTATTTTTGCGTTGGTTGATTTCCTCAATAATCAATGCAAACCGTTTTTCATTGAGTG             <  SRR4302543.4757531/94‑1 (MQ=60)
                                                                                  TTACGGAAG‑CCGTTTTTTCTTCTGTCGCCATCTCTTTATTTTTGCGTTGGTTGATTTCCTCAATAATCAATGCAAACCGTTTTTCATTGAGTGT            >  SRR4302543.1993538/1‑94 (MQ=60)
                                                                                  TTACGGAAG‑CCGTTTTTTCTTCTGTCGCCATCTCTTTATTTTTGCGTTGGTTGATTTCCTCAATAATCAATGCAAACCGTTTTTCATTGAGTGT            >  SRR4302543.7738421/1‑94 (MQ=60)
                                                                                   TACGGAAG‑CCGTTTTTTCTTCTGTCGCCATCTCTTTATTTTTGCGTTGGTTGATTTCCTCAATAATCAATGCAAACCGTTTTTCATTGAGTGTG           >  SRR4302543.5804993/1‑94 (MQ=60)
                                                                                   TACGGAAG‑CCGTTTTTTCTTCTGTCGCCATCTCTTTATTTTTGCGTTGGTTGATTTCCTCAATAATCAATGCAAACCGTTTTTCATTGAGTGTG           <  SRR4302543.500564/94‑1 (MQ=60)
                                                                                   TACGGAAG‑CCGTTTTTTCTTCTGTCGCCATCTCTTTATTTTTGCGTTGGTTGATTTCCTCAATAATCAATGCAAACCGTTTTTCATTGAGTGTG           <  SRR4302543.5072415/94‑1 (MQ=60)
                                                                                     CGGAAG‑CCGTTTTTTCTTCTGTCGCCATCTCTTTATTTTTGCGTTGGTTGATTTCCTCAATAATCAATGCAAACCGTTTTTCATTGAGTGTGTA         >  SRR4302543.7830753/1‑94 (MQ=60)
                                                                                      GGAAG‑CCGTTTTTTCTTCTGTCGCCATCTCTTTATTTTTGCGTTGGTTGATTTCCTCAATAATCAATGCAAACCGTTTTTCATTGAGTGTGTAA        >  SRR4302543.7211045/1‑94 (MQ=60)
                                                                                      GGAAG‑CCGTTTTTTCTTCTGTCGCCATCTCTTTATTTTTGCGTTGGTTGATTTCCTCAATAATCAATGCAAACCGTTTTTCATTGAGTGTGTAA        >  SRR4302543.2060133/1‑94 (MQ=60)
                                                                                      GGAAG‑CCGTTTTTTCTTCTGTCGCCATCTCTTTATTTTTGCGTTGGTTGATTTCCTCAATAATCAATGCAAACCGCTTGTCATTGTGTGTGTGA        >  SRR4302543.7211044/1‑94 (MQ=60)
                                                                                      GGAAG‑CCGTTTTTTCTTCTGACGCCATCTCTTTATTTTTGCGTTGGTTGATTTCCTCAATAATCAATGCAAACCGTTTTTCATTGAGTGTGTAA        >  SRR4302543.5163062/1‑94 (MQ=60)
                                                                                        AAG‑CCGTTTTTTCTTCTGTCGCCATCTCTTTATTTTTGCGTTGGTTGATTTCCTCAATAATCCATGCACACAGCTTTTCATTGAGTGTGTAAAA      >  SRR4302543.6729081/1‑94 (MQ=60)
                                                                                        AAG‑CCGTTTTTTCTTCAGTCGCCATCTCTTTATTTTTGCGTTGGTTGATTTCCTCAATAATCAATGCAAACCGTTTTTCATTGAGTGTGTAAAA      >  SRR4302543.7781578/1‑94 (MQ=60)
                                                                                          agCCGTTTTTTCTTCTGTCGCCATCTCTTTATTTTTGCGTTGGTTGATTTCCTCAATAATCAATGCAAACCGTTTTTCATTGAGTGTGTAAAAG     <  SRR4302543.221642/92‑1 (MQ=60)
                                                                                          agCCGTTTTTTCTTCTGTCGCCATCTCTTTATTTTTGCGTTGGTTGATTTCCTCAATAATCAATGCAAACCGTTTTTCATTGAGTGTGTAAAAG     >  SRR4302543.181862/3‑94 (MQ=60)
                                                                                          agCCGTTTTTTCTTCTGTCGCCATCTCTTTATTTTTGCGTTGGTTGATTTCCTCAATAATCAATGCAAACCGTTTTTCATTGAGTGTGTAAAAG     >  SRR4302543.7364804/3‑94 (MQ=60)
                                                                                           GCCGTTTTTTCTTCTGTCGCCATCTCTTTATTTTTGCGTTGGTTGATTTCCTCAATAATCAATGCAAACCGTTTTTCATTGAGTGTGTAAAAGA    <  SRR4302543.4474357/94‑1 (MQ=60)
                                                                                            CCGTTTTTTCTTCTGTCGCCATCTCTTTATTTTTGCGTTGGTTGATTTCCTCAATAATCAATGCAAACCGTTTTTCATTGAGTGTGTAAAAGAA   >  SRR4302543.2978666/1‑94 (MQ=60)
                                                                                             CGTTTTTTCTTCTGTCGCCATCTCTTTATTTTTGCGTTGGTTGATTTCCTCAATAATCAATGCAAACCGTTTTTCATTGAGTGTGTAAAAGAAT  >  SRR4302543.7759602/1‑94 (MQ=60)
                                                                                             CGTTTTTTCTTCTGTCGCCATCTCTTTATTTTTGCGTTGGTTGATTTCCTCAATAATCAATGCAAACCGTTTTTCATTGAGTGTGTAAAAGAAT  <  SRR4302543.7852903/94‑1 (MQ=60)
                                                                                             CGTTTTTTCTTCTGTCGCCATCTCTTTATTTTTGCGTTGGTTGATTTCCTCAATAATCAATGCAAACCGTTTTTCATTGAGTGTGTAAAAGAAT  >  SRR4302543.5451597/1‑94 (MQ=60)
                                                                                             CGTTTTTTCTTCTGTCGCCATCTCTTTATTTTTGCGTTGGTTGATTTCCTCAATAATCAATGCAAACCGTTTTTCATTGAGTGTGTAAAAGAAT  <  SRR4302543.5489082/94‑1 (MQ=60)
                                                                                             CGTTTTTTCTTCTGTCGCCATCTCTTTATTTTTGCGTTGGTTGATTTCCTCAATAATCAATGCAAACCGTTTTTCATTGAGTGTGTAAAAGAAT  <  SRR4302543.6580815/94‑1 (MQ=60)
                                                                                             CGTTTTTTCTTCTGTCGCCATCTCTTTATTTTTGCGTTGGTTGATTTCCTCAATAATCAATGCAAACCGTTTTTCATTGAGTGTGTAAAAGAAT  <  SRR4302543.6177920/94‑1 (MQ=60)
                                                                                             |                                                                                             
TTTTCATAATAAGTCCATTTTTTAAACGTAGGGAGGCCGCCACCCACCAGGATGGAAATAGCGGCTATTGACATTTATAACGTTACGGAAGCCCGTTTTTTCTTCTGTCGCCATCTCTTTATTTTTGCGTTGGTTGATTTCCTCAATAATCAATGCAAACCGTTTTTCATTGAGTGTGTAAAAGAAT  >  NC_000913/4061868‑4062054

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑