Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 1696 279.3 15874187 97.8% 15524954 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,221,526 A→G E40E (GAA→GAG)  metH → B12‑dependent homocysteine‑N5‑methyltetrahydrofolate transmethylase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,221,5260AG99.4% 1125.4 / ‑5.4 360E40E (GAA→GAG) metHB12‑dependent homocysteine‑N5‑methyltetrahydrofolate transmethylase
Reads supporting (aligned to +/- strand):  ref base A (2/0);  new base G (180/178);  total (182/178)
Fisher's exact test for biased strand distribution p-value = 4.99e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.38e-01

Only 100 of 376 total aligned reads displayed.

GCAGTTAAATGAACGTATTCTGGTGCTGGACGGCGGTATGGGCACCATGATCCAGAGTTATCGACTGAACGAAGCCGATTTTCGTGGTGAACGCTTTGCCGACTGGCCATGCGACCTCAAAGGCAACAACGACCTGCTGGTACTCAGTAAACCGGAAGTGATCGCCGCTATCCACAACGC  >  NC_000913/4221436‑4221615
                                                                                          |                                                                                         
gcAGTTAAATGAACGTATTCTGGTGCTGGACGGCGGTATGGGCACCATGATCCAGAGTTATCGACTGAACGAAGCCGATTTTCGTGGTGAGCg                                                                                         >  2:5761319/1‑93 (MQ=255)
gcAGTTAAATGAACGTATTCTGGTGCTGGACGGCGGTATGGGCACCATGATCCAGAGTTATCGACTGAACGAAGCCGATTTTCGTGGTGAGCg                                                                                         >  1:7317330/1‑93 (MQ=255)
gcAGTTAAATGAACGTATTCTGGTGCTGGACGGCGGTATGGGCACCATGATCCAGAGTTATCGACTGAACGAAGCCGATTTTCGTGGTGAGCg                                                                                         >  1:3031613/1‑93 (MQ=255)
    ttAAATGAACGTATTCTGGTGCTGGACGGCGGTATGGGCACCATGATCCAGAGTTATCGACTGAACGAAGCCGATTTTCGTGGTGAGCGCttt                                                                                     >  2:6145594/1‑93 (MQ=255)
    ttAAATGAACGTATTCTGGTGCTGGACGGCGGTATGGGCACCATGATCCAGAGTTATCGACTGAACGAAGCCGATTTTCGTGGTGAGCGCttt                                                                                     <  2:1146690/93‑1 (MQ=255)
     tAAAGGAACGTATGCTGGGTCTGGACGGCGGTATGGGCACCATGATCCAGAGTTATCGACTGCACCAAGCCGATTTTCGTGGTGAGCGCTTTg                                                                                    <  1:1647525/93‑1 (MQ=255)
      aaaTGAACGTATTCTGGTGCTGGACGGCGGTATGGGCACCATGATCCAGAGTTATCGACTGAACGAAGCCGATTTTCGTGGTGAGCGCTTTGc                                                                                   <  1:3982756/93‑1 (MQ=255)
      aaaTGAACGTATTCTGGTGCTGGACGGCGGTATGGGCACCATGATCCAGAGTTATCGACTGAACGAAGCCGATTTTCGTGGTGAGCGCTTTGc                                                                                   >  2:7343097/1‑93 (MQ=255)
       aaTGAACGTATTCTGGTGCTGGACGGCGGTATGGGCACCATGATCCAGAGTTATCGACTGAACGAAGCCGATTTTCGTGGTGAGCGCTTTGcc                                                                                  >  1:2259196/1‑93 (MQ=255)
       aaTGAACGTATTCTGGTGCTGGACGGCGGTATGGGCACCATGATCCAGAGTTATCGACTGAACGAAGCCGATTTTCGTGGTGAGCGCTTTGcc                                                                                  >  1:1540666/1‑93 (MQ=255)
       aaTGAACGTATTCTGGTGCTGGACGGCGGTATGGGCACCATGATCCAGAGTTATCGACTGAACGAAGCCGATTTTCGTGGTGAGCGCTTTGcc                                                                                  >  2:4633122/1‑93 (MQ=255)
       aaTGAACGTATTCTGGTGCTGGACGGCGGTATGGGCACCATGATCCAGAGTTATCGACTGAACGAAGCCGATTTTCGTGGTGAGCGCTTTGcc                                                                                  >  1:600664/1‑93 (MQ=255)
         tGAACGTATTCTGGTGCTGGACGGCGGTATGGGCACCATGATCCAGAGTTATCGACTGAACGAAGCCGATTTTCGTGGTGAGCGCTTTGCCGa                                                                                <  1:1372700/93‑1 (MQ=255)
          gAACGTATTCTGGTGCTGGACGGCGGTATGGGCACCATGATCCAGAGTTATCGACTGAACGAAGCCGATTTTCGTGGTGAGCGCTTTGCCGAc                                                                               >  2:6339863/1‑93 (MQ=255)
          gAACGTATTCTGGTGCTGGACGGCGGTATGGGCACCATGATCCAGAGTTATCGACTGAACGAAGCCGATTTTCGTGGTGAGCGCTTTGCCGAc                                                                               <  1:3241691/93‑1 (MQ=255)
             cGTATTCTGGTGCTGGACGGCGGTATGGGCACCATGATCCAGAGTTATCGACTGAACGAAGCCGATTTTCGTGGTGAGCGCTTTGCCGACggg                                                                            >  1:2187872/1‑93 (MQ=255)
             cGTATTCTGGTGCTGGACGGCGGTATGGGCACCATGATCCAGAGTTATCGACTGAACGAAGCCGATTTTCGTGGTGAGCGCTTTGCCGACTgg                                                                            >  2:2296581/1‑93 (MQ=255)
                 ttCTGGTGCTGGACGGCGGTATGGGCACCATGATCCAGAGTTATCGACTGAACGAAGCCGATTTTCGTGGTGAGCGCTTTGCCGACTGGCCAt                                                                        <  2:5050388/93‑1 (MQ=255)
                 ttCTGGTGCTGGACGGCGGTATGGGCACCATGATCCAGAGTTATCGACTGAACGAAGCCGATTTTCGTGGTGAGCGCTTTGCCGACTGGCCAt                                                                        >  1:4048954/1‑93 (MQ=255)
                 ttCTGGTGCTGGACGGCGGTATGGGCACCATGATCCAGAGTTATCGACTGAACGAAGCCGATTTTCGTGGTGAGCGCTTTGCCGACTGGCCAt                                                                        <  1:5592401/93‑1 (MQ=255)
                  tCTGGTGCTGGACGGCGGTATGGGCACCATGATCCAGAGTTATCGACTGAACGAAGCCGATTTTCGTGGTGAGCGCTTTGCCGACTGGCCATg                                                                       <  2:6920050/93‑1 (MQ=255)
                   cTGGTGCTGGACGGCGGTATGGGCACCATGATCCAGAGTTATCGACTGAACGAAGCCGATTTTCGTGGTGAGCGCTTTGCCGACTGGCCATGc                                                                      >  1:6647516/1‑93 (MQ=255)
                    tGGTGCTGGACGGCGGTATGGGCACCATGATCCAGAGTTATCGACTGAACGAAGCCGATTTTCGTGGTGAGCGCTTTGCCGACTGGCCATGCg                                                                     >  2:5199360/1‑93 (MQ=255)
                     ggTGCTGGACGGCGGTATGGGCACCATGATCCAGAGTTATCGACTGAACGAAGCCGATTTTCGTGGTGAGCGCTTTGCCGACTGGCCATGCGa                                                                    <  1:5038009/93‑1 (MQ=255)
                     ggTGCTGGACGGCGGTATGGGCACCATGATCCAGAGTTATCGACTGAACGAAGCCGATTTTCGTGGTGAGCGCTTTGCCGACTGGCCATGCGa                                                                    >  2:3146662/1‑93 (MQ=255)
                       tGCTGGACGGCGGTATGGGCACCATGATCCAGAGTTATCGACTGAACGAAGCCGATTTTCGTGGTGAGCGCTTTGCCGACTGGCCATGCGAcc                                                                  >  1:2596004/1‑93 (MQ=255)
                        gCTGGACGGCGGTATGGGCACCATGATCCAGAGTTATCGACTGAACGAAGCCGATTTTCGTGGTGAGCGCTTTGCCGACTGGCCATGCGAccc                                                                 >  1:1226106/1‑92 (MQ=255)
                        gCTGGACGGCGGTATGGGCACCATGATCCAGAGTTATCGACTGAACGAAGCCGATTTTCGTGGTGAGCGCTTTGCCGACTGGCCATGCGACCt                                                                 >  2:424639/1‑93 (MQ=255)
                        gCTGGACGGCGGTATGGGCACCATGATCCAGAGTTATCGACTGAACGAAGCCGATTTTCGTGGTGAGCGCTTTGCCGACTGGCCATGCGACCt                                                                 <  2:7718907/93‑1 (MQ=255)
                         cTGGACGGCGGTATGGGCACCATGATCCAGAGTTATCGACTGAACGAAGCCGATTTTCGTGGTGAGCGCTTTGCCGACTGGCCATGCGACCTc                                                                <  1:4217978/93‑1 (MQ=255)
                          tGGACGGCGGTATGGGCACCATGATCCAGAGTTATCGACTGAACGAAGCCGATTTTCGTGGTGAGCGCTTTGCCGACTGGCCATGCGACCTCa                                                               <  1:86623/93‑1 (MQ=255)
                           ggACGGCGGTATGGGCACCATGATCCAGAGTTATCGACTGAACGAAGCCGATTTTCGTGGTGAGCGCTTTGCCGACTGGCCATGCGACCTCaa                                                              <  1:5772125/93‑1 (MQ=255)
                            gACGGCGGTATGGGCACCATGATCCAGAGTTATCGACTGAACGAAGCCGATTTTCGTGGTGAGCGCTTTGCCGACTGGCCATGCGACCTCaaa                                                             <  1:4792699/93‑1 (MQ=255)
                            gACGGCGGTATGGGCACCATGATCCAGAGTTATCGACTGAACGAAGCCGATTTTCGTGGTGAGCGCTTTGCCGACTGGCCATGCGACCTCaaa                                                             <  2:3358164/93‑1 (MQ=255)
                            gACGGCGGTATGGGCACCATGATCCAGAGTTATCGACTGAACGAAGCCGATTTTCGTGGTGAGCGCTTTGCCGACTGGCCATGCGACCTCaaa                                                             <  2:4806347/93‑1 (MQ=255)
                              cggcggTATGGGCACCATGATCCAGAGTTATCGACTGAACGAAGCCGATTTTCGTGGTGAGCGCTTTGCCGACTGGCCATGCGACCTCAAAgg                                                           >  2:848947/1‑93 (MQ=255)
                               ggcggTATGGGCACCATGATCCAGAGTTATCGACTGAACGAAGCCGATTTTCGTGGTGAGCGCTTTGCCGACTGGCCATGCGACCTCAAAGGc                                                          <  2:6320220/93‑1 (MQ=255)
                               ggcggTATGGGCACCATGATCCAGAGTTATCGACTGAACGAAGCCGATTTTCGTGGTGAGCGCTTTGCCGACTGGCCATGCGACCTCAAAGGc                                                          <  2:2472314/93‑1 (MQ=255)
                                 cggTATGGGCACCATGATCCAGAGTTATCGACTGAACGAAGCCGATTTTCGTGGTGAGCGCTTTGCCGACTGGCCATGCGACCTCAAAGGcaa                                                        >  2:2021650/1‑93 (MQ=255)
                                 cggTATGGGCACCATGATCCAGAGTTATCGACTGAACGAAGCCGATTTTCGTGGTGAGCGCTTTGCCGACTGGCCATGCGACCTCAAAGGcaa                                                        <  2:6866388/93‑1 (MQ=255)
                                   gTATGGGCACCATGATCCAGAGTTATCGACTGAACGAAGCCGATTTTCGTGGTGAGCGCTTTGCCGACTGGCCATGCGACCTCAAAGGcaaca                                                      >  1:5144140/1‑93 (MQ=255)
                                   gTATGGGCACCATGATCCAGAGTTATCGACTGAACGAAGCCGATTTTCGTGGTGAGCGCTTTGCCGACTGGCCATGCGACCTCAAAGGcaaca                                                      >  1:6883484/1‑93 (MQ=255)
                                    tATGGGCACCATGATCCAGAGTTATCGACTGAACGAAGCCGATTTTCGTGGTGAGCGCTTTGCCGACTGGCCATGCGACCTCAAAGGcaacaa                                                     >  1:3326234/1‑93 (MQ=255)
                                    tATGGGCACCATGATCCAGAGTTATCGACTGAACGAAGCCGATTTTCGTGGTGAGCGCTTTGCCGACTGGCCATGCGACCTCAAAGGcaacaa                                                     >  1:371490/1‑93 (MQ=255)
                                     aTGGGCACCATGATCCAGAGTTATCGACTGAACGAAGCCGATTTTCGTGGTGAGCGCTTTGCCGACTGGCCATGCGACCTCAAAGGCaacaac                                                    <  1:2739650/93‑1 (MQ=255)
                                     aTGGGCACCATGATCCAGAGTTATCGACTGAACGAAGCCGATTTTCGTGGTGAGCGCTTTGCCGACTGGCCATGCGACCTCAAAGGCaacaac                                                    <  2:5912215/93‑1 (MQ=255)
                                      tGGGCACCATGATCCAGAGTTATCGACTGAACGAAGCCGATTTTCGTGGTGAGCGCTTTGCCGACTGGCCATGCGACCTCAAAGGCAACAACg                                                   >  2:4351655/1‑93 (MQ=255)
                                       gggCACCATGATCCAGAGTTATCGACTGAACGAAGCCGATTTTCGTGGTGAGCGCTTTGCCGACTGGCCATGCGACCTCAAAGGCAACAACGa                                                  <  1:18264/93‑1 (MQ=255)
                                        ggCACCATGATCCAGAGTTATCGACTGAACGAAGCCGATTTTCGTGGTGAGCGCTTTGCCGACTGGCCATGCGACCTCAAAGGCAACAACGAc                                                 <  2:4386943/93‑1 (MQ=255)
                                        ggCACCATGATCCAGAGTTATCGACTGAACGAAGCCGATTTTCGTGGTGAGCGCTTTGCCGACTGGCCATGCGACCTCAAAGGCAACAACGAc                                                 >  2:1726362/1‑93 (MQ=255)
                                         gCACCATGATCCAGAGTTATCGACTGAACGAAGCCGATTTTCGTGGTGAGCGCTTTGCCGACTGGCCATGCGACCTCAAAGGCAACAACGAcc                                                <  1:5082943/93‑1 (MQ=255)
                                          cACCATGATCCAGAGTTATCGACTGAACGAAGCCGATTTTCGTGGTGAGCGCTTTGCCGACTGGCCATGCGACCTCAAAGGCAACAACGACct                                               <  2:2910925/93‑1 (MQ=255)
                                             cATGATCCAGAGTTATCGACTGAACGAAGCCGATTTTCGTGGTGAGCGCTTTGCCGACTGGCCATGCGACCTCAAAGGCAACAACGACctgct                                            <  2:3650836/93‑1 (MQ=255)
                                             cATGATCCAGAGTTATCGACTGAACGAAGCCGATTTTCGTGGTGAGCGCTTTGCCGACTGGCCATGCGACCTCAAAGGCAACAACGACctgct                                            >  2:721445/1‑93 (MQ=255)
                                             cATGATCCAGAGTTATCGACTGAACGAAGCCGATTTTCGTGGTGAGCGCTTTGCCGACTGGCCATGCGACCTCAAAGGCAACAACGACctgct                                            <  1:6176115/93‑1 (MQ=255)
                                              aTGATCCAGAGTTATCGACTGAACGAAGCCGATTTTCGTGGTGAGCGCTTTGCCGACTGGCCATGCGACCTCAAAGGCAACAACGACctgctg                                           >  2:7506853/1‑93 (MQ=255)
                                               tGATCCAGAGTTATCGACTGAACGAAGCCGATTTTCGTGGTGAGCGCTTTGCCGACTGGCCATGCGACCTCAAAGGCAACAACGAGCTGCTgg                                          >  1:246197/1‑93 (MQ=255)
                                               tGATCCAGAGTTATCGACTGAACGAAGCCGATTTTCGTGGTGAGCGCTTTGCCGACTGGCCATGCGACCTCAAAGGCAACAACGACCTGCTgg                                          <  2:5550021/93‑1 (MQ=255)
                                                gATCCAGAGTTATCGACTGAACGAAGCCGATTTTCGTGGTGAGCGCTTTGCCGACTGGCCATGCGACCTCAAAGGCAACAACGACCTGCTGGt                                         >  2:4052649/1‑93 (MQ=255)
                                                 aTCCAGAGTTATCGACTGAACGAAGCCGATTTTCGTGGTGAGCGCTTTGCCGACTGGCCATGCGACCTCAAAGGCAACAACGACCTGCTGGTa                                        <  2:381216/93‑1 (MQ=255)
                                                 aTCCAGAGTTATCGACTGAACGAAGCCGATTTTCGTGGTGAGCGCTTTGCCGACTGGCCATGCGACCTCAAAGGCAACAACGACATGCTGGTa                                        >  2:6767455/1‑93 (MQ=255)
                                                   ccAGAGTTATCGACTGAACGAAGCCGATTTTCGTGGTGAGCGCTTTGCCGACTGGCCATGCGACCTCAAAGGCAACAACGACCTGCTGGTACt                                      <  2:900967/93‑1 (MQ=255)
                                                    cAGAGTTATCGACTGAACGAAGCCGATTTTCGTGGTGAGCGCTTTGCCGACTGGCCATGCGACCTCAAAGGCAACAACGACCTGCTGGTACTc                                     <  1:3597597/93‑1 (MQ=255)
                                                    cAGAGTTATCGACTGAACGAAGCCGATTTTCGTGGTGAGCGCTTTGCCGACTGGCCATGCGACCTCAAAGGCAACAACGACCTGCTGGTACTc                                     >  2:5279269/1‑93 (MQ=255)
                                                    cAGAGTTATCGACTGAACGAAGCCGATTTTCGTGGTGAGCGCTTTGCCGACTGGCCATGCGACCTCAAAGGCAACAACGACCTGCTGGTACTc                                     >  1:7183990/1‑93 (MQ=255)
                                                     agagTTATCGACTGAACGAAGCCGATTTTCGTGGTGAGCGCTTTGCCGACTGGCCATGCGACCTCAAAGGCAACAACGACCTGCTGGTACTCa                                    >  1:1951103/1‑93 (MQ=255)
                                                      gagTTATCGACTGAACGAAGCCGATTTTCGTGGTGAGCGCTTTGCCGACTGGCCATGCGACCTCAAAGGCAACAACGACCTGCTGGTACTCAg                                   <  1:4683461/93‑1 (MQ=255)
                                                        gTTATCGACTGAACGAAGCCGATTTTCGTGGTGAGCGCTTTGCCGACTGGCCATGCGACCTCAAAGGCAACAACGACCTGCTGGTACTCAGTa                                 <  2:6553536/93‑1 (MQ=255)
                                                        gTTATCGACTGAACGAAGCCGATTTTCGTGGTGAGCGCTTTGCCGACTGGCCATGCGACCTCAAAGGCAACAACGACCTGCTGGTACTCAGTa                                 >  2:1382726/1‑93 (MQ=255)
                                                         ttATCGACTGAACGAAGCCGATTTTCGTGGTGAGCGCTTTGCCGACTGGCCATGCGACCTCAAAGGCAACAACGACCTGCTGGTACTCAGTaa                                >  2:7114407/1‑93 (MQ=255)
                                                         ttATCGACTGAACGAAGCCGATTTTCGTGGTGAGCGCTTTGCCGACTGGCCATGCGACCTCAAAGGCAACAACGACCTGCTGGTACTCAGTaa                                >  1:1021978/1‑93 (MQ=255)
                                                             cGACTGAACGAAGCCGATTTTCGTGGTGAGCGCTTTGCCGACTGGCCATGCGACCTCAAAGGCAACAACGACCTGCTGGTACTCAGTAAACCg                            <  2:2998177/93‑1 (MQ=255)
                                                              gACTGAACGAAGCCGATTTTCGTGGTGAGCGCTTTGCCGACTGGCCATGCGACCTCAAAGGCAACAACGACCTGCTGGTACTCAGTAAACCgg                           >  1:5398008/1‑93 (MQ=255)
                                                               aCTGAACGAAGCCGATTTTCGTGGTGAGCGCTTTGCCGACTGGCCATGCGACCTCAAAGGCAACAACGACCTGCTGGTACTCAGTAAACCGGa                          >  2:6600974/1‑93 (MQ=255)
                                                               aCTGAACGAAGCCGATTTTCGTGGTGAGCGCTTTGCCGACTGGCCATGCGACCTCAAAGGCAACAACGACCTGCTGGTACTCAGTAAACCGGa                          >  1:1314214/1‑93 (MQ=255)
                                                               aCTGAACGAAGCCGATTTTCGTGGTGAGCGCTTTGCCGACTGGCCATGCGACCTCAAAGGCAACAACGACCTGCTGGTACTCAGTAAACCGGa                          <  1:2422890/93‑1 (MQ=255)
                                                                cTGAACGAAGCCGATTTTCGTGGTGAGCGCTTTGCCGACTGGCCATGCGACCTCAAAGGCAACAACGACCTGCTGGTACTCAGTAAACCGGaa                         >  2:2671174/1‑93 (MQ=255)
                                                                 tGAACGAAGCCGATTTTCGTGGTGAGCGCTTTGCCGACTGGCCATGCGACCTCAAAGGCAACAACGACCTGCTGGTACTCAGTAAACCGGAAg                        <  2:1974382/93‑1 (MQ=255)
                                                                  gAACGAAGCCGATTTTCGTGGTGAGCGCTTTGCCGACTGGCCATGCGACCTCAAAGGCAACAACGACCTGCTGGTACTCAGTAAACCGGAAGt                       >  1:3871605/1‑93 (MQ=255)
                                                                   aaCGAAGCCGATTTTCGTGGTGAGCGCTTTGCCGACTGGCCATGCGACCTCAAAGGCAACAACGACCTGCTGGTACTCAGTAAACCGGAAGTg                      <  2:3445890/93‑1 (MQ=255)
                                                                    aCGAAGCCGATTTTCGTGGTGAGCGCTTTGCCGACTGGCCATGCGACCTCAAAGGCAACAACGACCTGCTGGTACTCAGTAAACCGGAAGTGa                     <  1:5943370/93‑1 (MQ=255)
                                                                    aCGAAGCCGATTTTCGTGGTGAGCGCTTTGCCGACTGGCCATGCGACCTCAAAGGCAACAACGACCTGCTGGTACTCAGTAAACCGGAAGTGa                     >  1:7225049/1‑93 (MQ=255)
                                                                     cGAAGCCGATTTTCGTGGTGAGCGCTTTGCCGACTGGCCATGCGACCTCAAAGGCAACAACGACCTGCTGGTACTCAGTAAACCGGAAGTGAt                    <  2:3861319/93‑1 (MQ=255)
                                                                      gAAGCCGATTTTCGTGGTGAGCGCTTTGCCGACTGGCCATGCGACCTCAAAGGCAACAACGACCTGCTGGTACTCAGTAAACCGGAAGTGATc                   <  1:375775/93‑1 (MQ=255)
                                                                        aGCCGATTTTCGTGGTGAGCGCTTTGCCGACTGGCCATGCGACCTCAAAGGCAACAACGACCTGCTGGTACTCAGTAAACCGGAAGTGATcgc                 >  2:6285105/1‑93 (MQ=255)
                                                                        aGCCGATTTTCGTGGTGAGCGCTTTGCCGACTGGCCATGCGACCTCAAAGGCAACAACGACCTGCTGGTACTCAGTAAACCGGAAGTGATcgc                 >  2:5346273/1‑93 (MQ=255)
                                                                         gCCGATTTTCGTGGTGAGCGCTTTGCCGACTGGCCATGCGACCTCAAAGGCAACAACGACCTGCTGGTACTCAGTAAACCGGAAGTGATcgcc                >  2:1023484/1‑93 (MQ=255)
                                                                            gATTTTCGTGGTGAGCGCTTTGCCGACTGGCCATGCGACCTCAAAGGCAACAACGACCTGCTGGTACTCAGTAAACCGGAAGTGATCGCCGCt             >  1:7770600/1‑93 (MQ=255)
                                                                            gATTTTCGTGGTGAGCGCTTTGCCGACTGGACATGCGACCTCAAAGGCAACAACGCCATGCTGGTACTCAGTAAGCCGGAAGTGATGGCCGCt             >  2:3293699/1‑93 (MQ=255)
                                                                               tttCGTGGTGAGCGCTTTGCCGACTGGCCATGCGACCTCAAAGGCAACAACGACCTGCTGGTACTCAGTAAACCGGAAGTGATCGCCGCTATc          <  1:1097900/93‑1 (MQ=255)
                                                                               tttCGTGGTGAGCGCTGTGCCGACTGGCCATGCGACCTCAAAGGCAACAACGACCTGCTGGTACTCAGTAAACCGGAAGTGATCGCCGCTATc          <  1:6472112/93‑1 (MQ=255)
                                                                                  cGTGGTGAGCGCTTTGCCGACTGGCCATGCGACCTCAAAGGCAACAACGACCTGCTGGTACTCAGTAAACCGGAAGTGATCGCCGCTATCcac       <  1:4519694/93‑1 (MQ=255)
                                                                                  cGTGGTGAGCGCTTTGCCGACTGGCCATGCGACCTCAAAGGCAACAACGACCTGCTGGTACTCAGTAAACCGGAAGTGATCGCCGCTATCcac       <  1:3443006/93‑1 (MQ=255)
                                                                                    tggtgAGCGCTTTGCCGACTGGCCATGCGACCTCAAAGGCAACAACGACCTGCTGGTACTCAGTAAACCGGAAGTGATCGCCGCTATCCACaa     <  2:7272803/93‑1 (MQ=255)
                                                                                    gggtgAGCGCTTTGCCGACTGGCCATGCCACCTCAAAGGCAACAACGACCTGCTGGTACTCAGTAAACCGGAAGTGATCGCCGCTATCCACaa     <  1:7606309/92‑1 (MQ=255)
                                                                                     ggtgAGCGCTTTGCCGCCTGGCCATGCGACCTCAAAGGCAACAACGACCTGCTGGTACTCAGTAAACCGGAAGTGATCGCCGCTATCCACAAc    <  1:7004941/93‑1 (MQ=255)
                                                                                     ggtgAGCGCTTTGCCGACTGGCCATGCGACCTCAAAGGCAACAACGACCTGCTGGTACTCAGTAAACCGGAAGTGATCGCCGCTATCCACAAc    >  1:6261037/1‑93 (MQ=255)
                                                                                     ggtgAGCGCTTTGCCGACTGGCCATGCGACCTCAAAGGCAACAACGACCTGCTGGTACTCAGGACACCGGAAGCGATCGCCGCTATCCACAAc    >  1:6932538/1‑93 (MQ=255)
                                                                                      gtgAGCGCTTTGCCGACTGGCCATGCGACCTCAAAGGCAACAACGACCTGCTGGTACTCAGTAAACCGGAAGTGATCGCCGCTATCCACAACg   <  2:698422/93‑1 (MQ=255)
                                                                                       tgAGCGCTTTGCCGACTGGCCATGCGACCTCAAAGGCAACAACGACCTGCTGGTACTCAGTAAACCGGAAGTGATCGCCGCTATCCACAACGc  >  2:6667856/1‑93 (MQ=255)
                                                                                          |                                                                                         
GCAGTTAAATGAACGTATTCTGGTGCTGGACGGCGGTATGGGCACCATGATCCAGAGTTATCGACTGAACGAAGCCGATTTTCGTGGTGAACGCTTTGCCGACTGGCCATGCGACCTCAAAGGCAACAACGACCTGCTGGTACTCAGTAAACCGGAAGTGATCGCCGCTATCCACAACGC  >  NC_000913/4221436‑4221615

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
TGCGCAGTTAAATGAACGTATTCTGGTGCTGGACGGCGGTATGGGCACCATGATCCAGAGTTATCGACTGAACGAAGCCGATTTTCGTGGTGAACGCTTTGCCGACTGGCCATGCGACCTCAAAGGCAACAACGACCTGCTGGTACTCAGTAAACCGGAAGTGATCGCCGCTATCCACAACGCCTAC  >  NC_000913/4221433‑4221619
                                                                                             |                                                                                             
TGCGCAGTTAAATGAACGTATTCTGGTGCTGGACGGCGGTATGGGCACCATGATCCAGAGTTATCGACTGAACGAAGCCGATTTTCGTGGTGAG                                                                                               <  SRR4302543.4255649/94‑1 (MQ=60)
TGCGCAGTTAAATGAACGTATTCTGGTGCTGGACGGCGGTATGGGCACCATGATCCAGAGTTATCGACTGAACGAAGCCGATTTTCGTGGTGAG                                                                                               <  SRR4302543.675793/94‑1 (MQ=60)
 GCGCAGTTAAATGAACGTATTCTGGTGCTGGACGGCGGTATGGGCACCATGATCCAGAGTTATCGACTGAACGAAGCCGATTTTCGTGGTGAGC                                                                                              <  SRR4302543.7253005/94‑1 (MQ=60)
  CGCAGTTAAATGAACGTATTCTGGTGCTGGACGGCGGTATGGGCACCATGATCCAGAGTTATCGACTGAACGAAGCCGATTTTCGTGGTGAGCG                                                                                             >  SRR4302543.3058987/1‑94 (MQ=60)
  CGCAGTTAAATGAACGTATTCTGGTGCTGGACGGCGGTATGGGCACCATGATCCAGAGTTATCGACTGAACGAAGCCGATTTTCGTGGTGAGCG                                                                                             >  SRR4302543.2113837/1‑94 (MQ=60)
  CGCAGTTAAATGAACGTATTCTGGTGCTGGACGGCGGTATGGGCACCATGATCCAGAGTTATCGACTGAACGAAGCCGATTTTCGTGGTGAGCG                                                                                             >  SRR4302543.2234013/1‑94 (MQ=60)
  CGCAGTTAAATGAACGTATTCTGGTGCTGGACGGCGGTATGGGCACCATGATCCAGAGTTATCGACTGAACGAAGCCGATTTTCGTGGTGAGCG                                                                                             <  SRR4302543.7332193/94‑1 (MQ=60)
  CGCAGTTAAATGAACGTATTCTGGTGCTGGACGGCGGTATGGGCACCATGATCCAGAGTTATCGACTGAACGAAGCCGATTTTCGTGGTGAGCG                                                                                             >  SRR4302543.7387828/1‑94 (MQ=60)
  CGCAGTTAAATGAACGTATTCTGGTGCTGGACGGCGGTATGGGCACCATGATCCAGAGTTATCGACTGAACGAAGCCGATTTTCGTGGTGAGCG                                                                                             <  SRR4302543.1334077/94‑1 (MQ=60)
  CGCAGTTAAATGAACGTATTCTGGTGCTGGACGGCGGTATGGGCACCATGATCCAGAGTTATCGACTGAACGAAGCCGATTTTCGTGGTGAGCG                                                                                             >  SRR4302543.4713077/1‑94 (MQ=60)
  CGCAGTTAAATGAACGTATTCTGGTGCTGGACGGCGGTATGGGCACCATGATCCAGAGTTATCGACTGAACGAAGCCGATTTTCGTGGTGAGCG                                                                                             >  SRR4302543.2479619/1‑94 (MQ=60)
    CAGTTAAATGAATGTATTCTGGTGCTGGACGGCGGTATGGGCACCATGATCCAGAGTTATCGACTGAACGAAGCCGATTTTCGTGGTGAGCGCT                                                                                           >  SRR4302543.156631/1‑94 (MQ=60)
    CAGTTAAATGAACGTATTCTGGTGCTGGACGGCGGTATGGGCACCATGATCCAGAGTTATCGACTGAACGAAGCCGATTTTCGTGGTGAGCGCT                                                                                           >  SRR4302543.1066625/1‑94 (MQ=60)
    CAGTTAAATGAACGTATTCTGGTGCTGGACGGCGGTATGGGCACCATGATCCAGAGTTATCGACTGAACGAAGCCGATTTTCGTGGTGAGCGCT                                                                                           >  SRR4302543.1337097/1‑94 (MQ=60)
     AGTTAAATGAACGTATTCTGGTGCTGGACGGCGGTATGGGCACCATGATCCAGAGTTATCGACTGAACGAAGCCGATTTTCGTGGTGAGCGCTT                                                                                          >  SRR4302543.4570908/1‑94 (MQ=60)
     AGTTAAATGAACGTATTCTGGTGCTGGACGGCGGTATGGGCACCATGATCCAGAGTTATCGACTGAACGAAGCCGATTTTCGTGGTGAGCGCTT                                                                                          >  SRR4302543.1084291/1‑94 (MQ=60)
     AGTTAAATGAACGTATTCTGGTGCTGGACGGCGGTATGGGCACCATGATCCAGAGTTATCGACTGAACGAAGCCGATTTTCGTGGTGAGCGCTT                                                                                          >  SRR4302543.5529602/1‑94 (MQ=60)
      GTTAAATGAACGTATTCTGGTGCTGGACGGCGGTATGGGCACCATGATCCAGAGTTATCGACTGAACGAAGCCGATTTTCGTGGTGAGCGCTTT                                                                                         >  SRR4302543.6159456/1‑94 (MQ=60)
      GTTAAATAAACGTATTCTGGTGCTGGACGGCGGTATGGGCACCATGATCCAGAGTTATCGACTGAACGAAGCCGATTTTCGTGGTGAGCGCTTT                                                                                         <  SRR4302543.3473391/94‑1 (MQ=60)
       TTAAATGAACGTATTCTGGTGCTGGACGGCGGTATGGGCACCATGATCCAGAGTTATCGACTGAACGAAGCCGATTTTCGTGGTGAGCGCTTTG                                                                                        <  SRR4302543.2639254/94‑1 (MQ=60)
        TAAAGGAACGTATGCTGGGTCTGGACGGCGGTATGGGCACCATGATCCAGAGTTATCGACTGCACCAAGCCGATTTTCGTGGTGAGCGCTTTGC                                                                                       <  SRR4302543.1662257/94‑1 (MQ=60)
         AAATGAACGTATTCTGGTGCTGGACGGCGGTATGGGCACCATGATCCAGAGTTATCGACTGAACGAAGCCGATTTTCGTGGTGAGCGCTTTGCC                                                                                      >  SRR4302543.606163/1‑94 (MQ=60)
         AAATGAACGTATTCTGGTGCTGGACGGCGGTATGGGCACCATGATCCAGAGTTATCGACTGAACGAAGCCGATTTTCGTGGTGAGCGCTTTGCC                                                                                      >  SRR4302543.1554463/1‑94 (MQ=60)
         AAATGAACGTATTCTGGTGCTGGACGGCGGTATGGGCACCATGATCCAGAGTTATCGACTGAACGAAGCCGATTTTCGTGGTGAGCGCTTTGCC                                                                                      >  SRR4302543.2279784/1‑94 (MQ=60)
         AAATGAACGTATTCTGGTGCTGGACGGCGGTATGGGCACCATGATCCAGAGTTATCGACTGAACGAAGCCGATTTTCGTGGTGAGCGCTTTGCC                                                                                      <  SRR4302543.4018084/94‑1 (MQ=60)
          AATGAACGTATTCTGGTGCTGGACGGCGGTATGGGCACCATGATCCAGAGTTATCGACTGAACGAAGCCGATTTTCGTGGTGAGCGCTTTGCCG                                                                                     <  SRR4302543.4971958/94‑1 (MQ=60)
          AATGAACGTATTCTGGTGCTGGACGGCGGTATGGGCACCATGATCCAGAGTTATCGACTGAACGAAGCCGATTTTCGTGGTGAGCGCTTTGCCG                                                                                     <  SRR4302543.3925156/94‑1 (MQ=60)
          AATGAACGTATTCTGGTGCTGGACGGCGGTATGGGCACCATGATCCAGAGTTATCGACTGAACGAAGCCGATTTTCGTGGTGAGCGCTTTGCCG                                                                                     <  SRR4302543.5166273/94‑1 (MQ=60)
           ATGAACGTATTCTGGTGCTGGACGGCGGTATGGGCACCATGATCCAGAGTTATCGACTGAACGAAGCCGATTTTCGTGGTGAGCGCTTTGCCGA                                                                                    >  SRR4302543.2955376/1‑94 (MQ=60)
           ATGAACGTATTCTGGTGCTGGACGGCGGTATGGGCACCATGATCCAGAGTTATCGACTGAACGAAGCCGATTTTCGTGGTGAGCGCTTTGCCGA                                                                                    >  SRR4302543.6828194/1‑94 (MQ=60)
           ATGAACGTATTCTGGTGCTGGACGGCGGTATGGGCACCATGATCCAGAGTTATCGACTGAACGAAGCCGATTTTCGTGGTGAGCGCTTTGCCGA                                                                                    >  SRR4302543.3577658/1‑94 (MQ=60)
           ATGAACGTATTCTGGTGCTGGACGGCGGTATGGGCACCATGATCCAGAGTTATCGACTGAACGAAGCCGATTTTCGTGGTGAGCGCTTTGCCGA                                                                                    >  SRR4302543.1323325/1‑94 (MQ=60)
            TGAACGTATTCTGGTGCTGGACGGCGGTATGGGCACCATGATCCAGAGTTATCGACTGAACGAAGCCGATTTTCGTGGTGAGCGCTTTGCCGAC                                                                                   <  SRR4302543.1385034/94‑1 (MQ=60)
            TGAACGTATTCTGGTGCTGGACGGCGGTATGGGCACCATGATCCAGAGTTATCGACTGAACGAAGCCGATTTTCGTGGTGAGCGCTTTGCCGAC                                                                                   >  SRR4302543.4624484/1‑94 (MQ=60)
             GAACGTATTCTGGTGCTGGACGGCGGTATGGGCACCATGATCCAGAGTTATCGACTGAACGAAGCCGATTTTCGTGGTGAGCGCTTTGCCGACT                                                                                  <  SRR4302543.3271011/94‑1 (MQ=60)
             GAACGTATTCTGGTGCTGGACGGCGGTATGGGCACCATGATCCAGAGTTATCGACTGAACGAAGCCGATTTTCGTGGTGAGCGCTTTGCCGACT                                                                                  >  SRR4302543.3418797/1‑94 (MQ=60)
             GAACGTATTCTGGTGCTGGACGGCGGTATGGGCACCATGATCCAGAGTTATCGACTGAACGAAGCCGATTTTCGTGGTGAGCGCTTTGCCGACT                                                                                  >  SRR4302543.5574018/1‑94 (MQ=60)
             GAACGTATTCTGGTGCTGGACGGCGGTATGGGCACCATGATCCAGAGTTATCGACTGAACGAAGCCGATTTTCGTGGTGAGCGCTTTGCCGACT                                                                                  >  SRR4302543.3641436/1‑94 (MQ=60)
             GAACGTATTCTGGTGCTGGACGGCGGTATGGGCACCATGATCCAGAGTTATCGACTGAACGAAGCCGATTTTCGTGGTGAGCGCTTTGCCGACT                                                                                  <  SRR4302543.6259597/94‑1 (MQ=60)
              AACGTATTCTGGTGCTGGACGGCGGTATGGGCACCATGATCCAGAGTTATCGACTGAACGAAGCCGATTTTCGTGGTGAGCGCTTTGCCGACTG                                                                                 <  SRR4302543.1305592/94‑1 (MQ=60)
               ACGTATTCTGGTGCTGGACGGCGGTATGGGCACCATGATCCAGAGTTATCGACTGAACGAAGCCGATTTTCGTGGTGAGCGCTTTGCCGACGGG                                                                                >  SRR4302543.2207502/1‑94 (MQ=60)
                CGTATTCTGGTGCTGGACGGCGGTATGGGCACCATGATCCAGAGTTATCGACTGAACGAAGCCGATTTTCGTGGTGAGCGCTTTGCCGACTGGC                                                                               >  SRR4302543.3282337/1‑94 (MQ=60)
                CGTATTCTGGTGCTGGACGGCGGTATGGGCACCATGATCCAGAGTTATCGACTGAACGAAGCCGATTTTCGTGGTGAGCGCTTTGCCGACTGGC                                                                               >  SRR4302543.6305959/1‑94 (MQ=60)
                 GTATTCTGGTGCTGGACGGCGGTATGGGCACCATGATCCAGAGTTATCGACTGAACGAAGCCGATTTTCGTGGTGAGCGCTTTGCCGACTGGCC                                                                              >  SRR4302543.6184806/1‑94 (MQ=60)
                 GTATTCTGGTGCTGGACGGCGGTATGGGCACCATGATCCAGAGTTATCGACTGAACGAAGCCGATTTTCGTGGTGAGCGCTTTGCCGACTGGCC                                                                              >  SRR4302543.2668956/1‑94 (MQ=60)
                 GTATTCTGGTGCTGGACGGCGGTATGGGCACCATGATCCAGAGTTATCGACTGAACGAAGCCGATTTTCGTGGTGAGCGCTTTGCCGACTGGCC                                                                              >  SRR4302543.1598913/1‑94 (MQ=60)
                 GTATTCTGGTGCTGGACGGCGGTATGGGCACCATGATCCAGAGTTATCGACTGAACGAAGCCGATTTTCGTGGTGAGCGCTTTGCCGACTGGCC                                                                              >  SRR4302543.860015/1‑94 (MQ=60)
                 GTATTCTGGTGCTGGACGGCGGTATGGGCACCATGATCCAGAGTTATCGACTGAACGAAGCCGATTTTCGTGGTGAGCGCTTTGCCGACTGGCC                                                                              >  SRR4302543.7699811/1‑94 (MQ=60)
                   ATTCTGGTGCTGGACGGCGGTATGGGCACCATGATCCAGAGTTATCGACTGAACGAAGCCGATTTTCGTGGTGAGCGCTTTGCCGACTGGCCAT                                                                            >  SRR4302543.4085185/1‑94 (MQ=60)
                   ATTCTGGTGCTGGACGGCGGTATGGGCACCATGATCCAGAGTTATCGACTGAACGAAGCCGATTTTCGTGGTGAGCGCTTTGCCGACTGGCCAT                                                                            >  SRR4302543.7121188/1‑94 (MQ=60)
                   ATTCTGGTGCTGGACGGCGGTATGGGCACCATGATCCAGAGTTATCGACTGAACGAAGCCGATTTTCGTGGTGAGCGCTTTGCCGACTGGCCAT                                                                            <  SRR4302543.6018055/94‑1 (MQ=60)
                   ATTCTGGTGCTGGACGGCGGTATGGGCACCATGATCCAGAGTTATCGACTGAACGAAGCCGATTTTCGTGGTGAGCGCTTTGCCGACTGGCCAT                                                                            >  SRR4302543.6617288/1‑94 (MQ=60)
                    TTCTGGTGCTGGACGGCGGTATGGGCACCATGATCCAGAGTTATCGACTGAACGAAGCCGATTTTCGTGGTGAGCGCTTTGCCGACTGGCCATG                                                                           <  SRR4302543.5643343/94‑1 (MQ=60)
                    TTCTGGTGCTGGACGGCGGTATGGGCACCATGATCCAGAGTTATCGACTGAACGAAGCCGATTTTCGTGGTGAGCGCTTTGCCGACTGGCCATG                                                                           >  SRR4302543.2435268/1‑94 (MQ=60)
                     TCTGGTGCTGGACGGCGGTATGGGCACCATGATCCAGAGTTATCGACTGAACGAAGCCGATTTTCGTGGTGAGCGCTTTGCCGACTGGCCATGC                                                                          >  SRR4302543.6710259/1‑94 (MQ=60)
                      CTGGTGCTGGACGGCGGTATGGGCACCATGATCCAGAGTTATCGACTGAACGAAGCCGATTTTCGTGGTGAGCGCTTTGCCGACTGGCCATGCG                                                                         >  SRR4302543.397164/1‑94 (MQ=60)
                      CTGGTGCTGGACGGCGGTATGGGCACCATGATCCAGAGTTATCGACTGAACGAAGCCGATTTTCGTGGTGAGCGCTTTGCCGACTGGCCATGCG                                                                         >  SRR4302543.7645251/1‑94 (MQ=60)
                      CTGGTGCTGGACGGCGGTATGGGCACCATGATCCAGAGTTATCGACTGAACGAAGCCGATTTTCGTGGTGAGCGCTTTGCCGACTGGCCATGCG                                                                         <  SRR4302543.3585426/94‑1 (MQ=60)
                        GGTGCTGGACGGCGGTATGGGCACCATGATCCAGAGTTATCGACTGAACGAAGCCGATTTTCGTGGTGAGCGCTTTGCCGACTGGCCATGCGAC                                                                       >  SRR4302543.227434/1‑94 (MQ=60)
                        GGTGCTGGACGGCGGTATGGGCACCATGATCCAGAGTTATCGACTGAACGAAGCCGATTTTCGTGGTGAGCGCTTTGCCGACTGGCCATGCGAC                                                                       <  SRR4302543.5083832/94‑1 (MQ=60)
                         GTGCTGGACGGCGGTATGGGCACCATGATCCAGAGTTATCGACTGAACGAAGCCGATTTTCGTGGTGAGCGCTTTGCCGACTGGCCATGCGACC                                                                      >  SRR4302543.2619590/1‑94 (MQ=60)
                          TGCTGGACGGCGGTATGGGCACCATGATCCAGAGTTATCGACTGAACGAAGCCGATTTTCGTGGTGAGCGCTTTGCCGACTGGCCATGCGACCT                                                                     <  SRR4302543.3208263/94‑1 (MQ=60)
                          TGCTGGACGGCGGTATGGGCACCATGATCCAGAGTTATCGACTGAACGAAGCCGATTTTCGTGGTGAGCGCTTTGCCGACTGGCCATGCGACCC                                                                     >  SRR4302543.1237123/1‑94 (MQ=60)
                           GCTGGACGGCGGTATGGGCACCATGATCCAGAGTTATCGACTGAACGAAGCCGATTTTCGTGGTGAGCGCTTTGCCTACTGGCCATGCGACCTC                                                                    >  SRR4302543.3763149/1‑94 (MQ=60)
                            CTGGACGGCGGTATGGGCACCATGATCCAGAGTTATCGACTGAACGAAGCCGATTTTCGTGGTGAGCGCTTTGCCGACTGGCCATGCGACCTCA                                                                   >  SRR4302543.6746676/1‑94 (MQ=60)
                            CTGGACGGCGGTATGGGCACCATGATCCAGAGTTATCGACTGAACGAAGCCGATTTTCGTGGTGAGCGCTTTGCCGACTGGCCATGCGACCTCA                                                                   >  SRR4302543.568422/1‑94 (MQ=60)
                            CTGGACGGCGGTATGGGCACCATGATCCAGAGTTATCGACTGAACGAAGCCGATTTTCGTGGTGAGCGCTTTGCCGACTGGCCATGCGACCTCA                                                                   <  SRR4302543.4256217/94‑1 (MQ=60)
                             TGGACGGCGGTATGGGCACCATGATCCAGAGTTATCGACTGAACGAAGCCGATTTTCGTGGTGAGCGCTTTGCCGACTGGCCATGCGACCTCAA                                                                  >  SRR4302543.1762067/1‑94 (MQ=60)
                             TGGACGGCGGTATGGGCACCATGATCCAGAGTTATCGACTGAACGAAGCCGATTTTCGTGGTGAGCGCTTTGCCGACTGGCCATGCGACCTCAA                                                                  <  SRR4302543.87420/94‑1 (MQ=60)
                             TGGACGGCGGTATGGGCACCATGATCCAGAGTTATCGACTGAACGAAGCCGATTTTCGTGGTGAGCGCTTTGCCGACTGGCCATGCGACCTCAA                                                                  >  SRR4302543.3333747/1‑94 (MQ=60)
                              GGACGGCGGTATGGGCACCATGATCCAGAGTTATCGACTGAACGAAGCCGATTTTCGTGGTGAGCGCTTTGCCGACTGGCCATGCGACCTCAAA                                                                 <  SRR4302543.6446972/94‑1 (MQ=60)
                              GGACGGCGGTATGGGCACCATGATCCAGAGTTATCGACTGAACGAAGCCGATTTTCGTGGTGAGCGCTTTGCCGACTGGCCATGCGACCTCAAA                                                                 <  SRR4302543.5824672/94‑1 (MQ=60)
                               GACGGCGGTATGGGCACCATGATCCAGAGTTATCGACTGAACGAAGCCGATTTTCGTGGTGAGCGCTTTGCCGACTGGCCATGCGACCTCAAAG                                                                <  SRR4302543.4836505/94‑1 (MQ=60)
                                GCGGCGGTATGGGCACCATGATCCAGAGTTATCGACTGAACGAAGCCGATTTTCGTGGTGAGCGCTTTGCCGACTGGCCATGCGACCTCAAAGG                                                               <  SRR4302543.5814204/94‑1 (MQ=60)
                                ACGGCGGTATGGGCACCATGATCCAGAGTTATCGACTGAACGAAGCCGATTTTCGTGGTGAGCGCTTTGCCGACTGGCCATGCGACCTCAAAGG                                                               <  SRR4302543.726141/94‑1 (MQ=60)
                                 CGGCGGTATGGGCACCATGATCCAGAGTTATCGACTGAACGAAGCCGATTTTCGTGGTGAGCGCTTTGCGGACTGGCCATGCGACCTCAAAGGA                                                              >  SRR4302543.1693891/1‑94 (MQ=60)
                                  GGCGGTATGGGCACCATGATCCAGAGTTATCGACTGAACGAAGCCGATTTTCGTGGTGAGCGCTTTGCCGACTGGCCATGCGACCTCAAAGGCA                                                             <  SRR4302543.4165828/94‑1 (MQ=60)
                                   GCGGTATGGGCACCATGATCCAGAGTTATCGACTGAACGAAGCCGATTTTCGTGGTGAGCGCTTTGCCGACTGGCCATGCGACCTCAAAGGCAA                                                            <  SRR4302543.6163203/94‑1 (MQ=60)
                                   GCGGTATGGGCACCATGATCCAGAGTTATCGACTGAACGAAGCCGATTTTCGTGGTGAGCGCTTTGCCGACTGGCCATGCGACCTCAAAGGCAA                                                            >  SRR4302543.2340437/1‑94 (MQ=60)
                                    CGGTATGGGCACCATGATCCAGAGTTATCGACTGAACGAAGCCGATTTTCGTGGTGAGCGCTTTGCCGACTGGCCATGCGACCTCAAAGGCAAC                                                           <  SRR4302543.1375262/94‑1 (MQ=60)
                                    CGGTATGGGCACCATGATCCAGAGTTATCGACTGAACGAAGCCGATTTTCGTGGTGAGCGCTTTGCCGACTGGCCATGCGACCTCAAAGGCAAC                                                           >  SRR4302543.7901781/1‑94 (MQ=60)
                                     GGTATGGGCACCATGATCCAGAGTTATCGACTGAACGAAGCCGATTTTCGTGGTGAGCGCTTTGCCGACTGTCCATGCGACCTCAAAGGCACCA                                                          >  SRR4302543.6341416/1‑94 (MQ=60)
                                     GGTATGGGCACCATGATCCAGAGTTATCGACTGAACGAAGCCGATTTTCGTGGTGAGCGCTTTGCCGACTGGCCATGCGACCTCAAAGGCAACA                                                          <  SRR4302543.2911366/94‑1 (MQ=60)
                                     GGTATGGGCACCATGATCCAGAGTTATCGACTGAACGAAGCCGATTTTCGTGGTGAGCGCTTTGCCGACTGGCCATGCGACCTCAAAGGCAACA                                                          >  SRR4302543.3555848/1‑94 (MQ=60)
                                     GGTATGGGCACCATGATCCAGAGTTATCGACTGAACGAAGCCGATTTTCGTGGTGAGCGCTTTGCCGACTGGCCATGCGACCTCAAAGGCAACA                                                          >  SRR4302543.6948242/1‑94 (MQ=60)
                                     GGTATGGGCACCATGATCCAGAGTTATCGACTGAACGAAGCCGATTTTCGTGGTGAGCGCTTTGCCGACTGGCCATGCGACCTCAAAGGCAACA                                                          >  SRR4302543.5190649/1‑94 (MQ=60)
                                      GTATGGGCACCATGATCCAGAGTTATCGACTGAACGAAGCCGATTTTCGTGGTGAGCGCTTTGCCGACTGGCCATGCGACCTCAAAGGCAACAA                                                         >  SRR4302543.5124615/1‑94 (MQ=60)
                                      GTATGGGCACCATGATCCAGAGTTATCGACTGAACGAAGCCGATTTTCGTGGTGAGCGCTTTGCCGACTGGCCATGCGACCTCAAAGGCAACAA                                                         >  SRR4302543.374912/1‑94 (MQ=60)
                                      GTATGGGCACCATGATCCAGAGTTATCGACTGAACGAAGCCGATTTTCGTGGTGAGCGCTTTGCCGACTGGCCATGCGACCTCAAAGGCAACAA                                                         >  SRR4302543.3356237/1‑94 (MQ=60)
                                       TATGGGCACCATGATCCAGAGTTATCGACTGAACGAAGCCGATTTTCGTGGTGAGCGCTTTGCCGACTGGCCATGCGACCTCAAAGGCAACAAC                                                        <  SRR4302543.1159745/94‑1 (MQ=60)
                                        ATGGGCACCATGATCCAGAGTTATCGACTGAACGAAGCCGATTTTCGTGGTGAGCGCTTTGCCGACTGGCCATGCGACCTCAAAGGCAACAACG                                                       <  SRR4302543.2764572/94‑1 (MQ=60)
                                        ATGGGCACCATGATCCAGAGTTATCGACTGAACGAAGCCGATTTTCGTGGTGAGCGCTTTGCCGACTGGCCATGCGACCTCAAAGGCAACAACG                                                       >  SRR4302543.7770834/1‑94 (MQ=60)
                                        ATGGGCACCATGATCCAGAGTTATCGACTGAACGAAGCCGATTTTCGTGGTGAGCGCTTTGCCGACTGGCCATGCGACCTCAAAGGCAACAACG                                                       >  SRR4302543.4503174/1‑94 (MQ=60)
                                          GGGCACCATGATCCAGAGTTATCGACTGAACGAAGCCGATTTTCGTGGTGAGCGCTTTGCCGACTGGCCATGCGACCTCAAAGGCAACAACGAC                                                     <  SRR4302543.18434/94‑1 (MQ=60)
                                           GGCACCATGATCCAGAGTTATCGACTGAACGAAGCCGATTTTCGTGGTGAGCGCTTTGCCGACTGGCCATGCGACCTCAAAGGCAACAACGACC                                                    <  SRR4302543.7371446/94‑1 (MQ=60)
                                            GCACCATGATCCAGAGTTATCGACTGAACGAAGCCGATTTTCGTGGTGAGCGCTTTGCCGACTGGCCATGCGACCTCAAAGGCAACAACGACCT                                                   <  SRR4302543.5129034/94‑1 (MQ=60)
                                            GCACCATGATCCAGAGTTATCGACTGAACGAAGCCGATTTTCGTGGTGAGCGCTTTGCCGACTGGCCATGCGACCTCAAAGGCAACAACGACCT                                                   <  SRR4302543.939988/94‑1 (MQ=60)
                                            GCACCATGATCCAGAGTTATCGACTGAACGAAGCCGATTTTCGTGGTGAGCGCTTTGCCGACTGGCCATGCGACCTCAAAGGCAACAACGACCT                                                   >  SRR4302543.1896061/1‑94 (MQ=60)
                                            GCACCATGATCCAGAGTTATCGACTGAACGAAGCCGATTTTCGTGGTGAGCGCTTTGCCGACTGGCCATGCGACCTCAAAGGCAACAACGACCT                                                   <  SRR4302543.3415581/94‑1 (MQ=60)
                                             CACCATGATCCAGAGTTATCGACTGAACGAAGCCGATTTTCGTGGTGAGCGCTTTGCCGACTGGCCATGCGACCTCAAAGGCAACAACGACCTG                                                  >  SRR4302543.5884776/1‑94 (MQ=60)
                                             CACCATGATCCAGAGTTATCGACTGAACGAAGCCGATTTTCGTGGTGAGCGCTTTGCCGACTGGCCATGCGACCTCAAAGGCAACAACGACCTG                                                  <  SRR4302543.3064931/94‑1 (MQ=60)
                                             CACCATGATCCAGAGTTATCGACTGAACGAAGCCGATTTTCGTGGTGAGCGCTTTGCCGACTGGCCATGCGACCTCAAAGGCAACAACGACCTG                                                  <  SRR4302543.5111511/94‑1 (MQ=60)
                                              ACCATGATCCAGAGTTATCGACTGAACGAAGCCGATTTTCGTGGTGAGCGCTTTGCCGACTGGCCATGCGACCTCAAAGGCAACAACGACCTGC                                                 >  SRR4302543.3781040/1‑94 (MQ=60)
                                               CCATGATCCAGAGTTATCGACTGAACGAAGCCGATTTTCGTGGTGAGCGCTTTGCCGACTGGCCATGCGACCTCAAAGGCAACAACGACCTGCT                                                >  SRR4302543.7114666/1‑94 (MQ=60)
                                                CATGATCCAGAGTTATCGACTGAACGAAGCCGATTTTCGTGGTGAGCGCTTTGCCGACTGGCCATGCGACCTCAAAGGCAACAACGACCTGCTG                                               <  SRR4302543.6003091/94‑1 (MQ=60)
                                                CATGATCCAGAGTTATCGACTGAACGAAGCCGATTTTCGTGGTGAGCGCTTTGCCGACTGGCCATGCGACCTCAAAGGCAACAACGACCTGCTG                                               >  SRR4302543.5131972/1‑94 (MQ=60)
                                                CATGATCCAGAGTTATCGACTGAACGAAGCCGATTTTCGTGGTGAGCGCTTTGCCGACTGGCCATGCGACCTCAAAGGCAACAACGACCTGCTG                                               >  SRR4302543.5568295/1‑94 (MQ=60)
                                                CATGATCCAGAGTTATCGACTGAACGAAGCCGATTTTCGTGGTGAGCGCTTTGCCGACTGGCCATGCGACCTCAAAGGCAACAACGACCTGCTG                                               <  SRR4302543.6234512/94‑1 (MQ=60)
                                                CATGATCCAGAGTTATCGACTGAACGAAGCCGATTGTCGTGGTGAGCGCTTTGCCGACTGGCCATGCGACCTCAAAGGCAACAACGACCTGCTG                                               <  SRR4302543.3633504/94‑1 (MQ=60)
                                                 ATGATCCAGAGTTATCGACTGAACGAAGCCGATTTTCGTGGTGAGCGCTTTGCCGACTGGCCATGCGACCTCAAAGGCAACAACGAGCTGCTGG                                              >  SRR4302543.248335/1‑94 (MQ=60)
                                                 ATGATCCAGAGTTATCGACTGAACGAAGCCGATTTTCGTGGTGAGCGCTTTGCCGACTGGCCATGCGACCTCAAAGGCAACAACGACCTGCTGG                                              >  SRR4302543.759019/1‑94 (MQ=60)
                                                 ATGATCCAGAGTTATCGACTGAACGAAGCCGATTTTCGTGGTGAGCGCTTTGCCGACTGGCCATGCGACCTCAAAGGCAACAACGACCTGCTGG                                              <  SRR4302543.6482553/94‑1 (MQ=60)
                                                  TGATCCAGAGTTATCGACTGAACGAAGCCGATTTTCGTGGTGAGCGCTTTGCCGACTGGCCATGCGACCTCAAAGGCAACAACGACCTGCTGGT                                             <  SRR4302543.601408/94‑1 (MQ=60)
                                                   GATCCAGAGTTATCGACTGAACGAAGCCGATTTTCGTGGTGAGCGCTTTGCCGACTGGCCATGCGACCTCAAAGGCAACAACGACCTGCTGGTA                                            <  SRR4302543.7329172/94‑1 (MQ=60)
                                                   GATCCAGAGTTATCGACTGAACGAAGCCGATTTTCGTGGTGAGCGCTTTGCCGACTGGCCATGCGACCTCAAAGGCAACAACGACCTGCTGGTA                                            >  SRR4302543.3873351/1‑94 (MQ=60)
                                                   GATCCAGAGTTATCGACTGAACGAAGCCGATTTTCGTGGTGAGCGCTTTGCCGACTGGCCATGCGACCTCAAAGGCAACAACGACCTGCTGGTA                                            <  SRR4302543.4774014/94‑1 (MQ=60)
                                                    ATCCAGAGTTATCGACTGAACGAAGCCGATTTTCATGGTGAGCGCTTTGCCGACTGGCCATGCGACCTCAAAGGCACCAACGACCTGCTGGTAC                                           >  SRR4302543.2509381/1‑94 (MQ=60)
                                                      CCAGAGTTATCGACTGAACGAAGCCGATTTTCGTGGTGAGCGCTTTGCCGACTGGCCATGCGACCTCAAAGGCAACAACGACCTGCTGGTACTC                                         >  SRR4302543.7253698/1‑94 (MQ=60)
                                                      CCAGAGTTATCGACTGAACGAAGCCGATTTTCGTGGTGAGCGCTTTGCCGACTGGCCATGCGACCTCAAAGGCAACAACGACCTGCTGGTACTC                                         >  SRR4302543.5333711/1‑94 (MQ=60)
                                                       CAGAGTTATCGACTGAACGAAGCCGATTTTCGTGGTGAGCGCTTTGCCGACTGGCCATGCGACCTTAAAGGCAACAACGACCTGCTGGTACTCA                                        >  SRR4302543.1509047/1‑94 (MQ=60)
                                                       CAGAGTTATCGACTGAACGAAGCCGATTTTCGTGGTGAGCGCTTTGCCGACTGGCCATGCGACCTCAAAGGCAACAACGACCTGCTGGTACTCA                                        >  SRR4302543.1968600/1‑94 (MQ=60)
                                                       CAGAGTTATCGACTGAACGAAGCCGATTTTCGTGGTGAGCGCTTTGCCGACTGGCCATGCGACCTCAAAGGCAACAACGACCTGCTGGTACTCA                                        <  SRR4302543.3629346/94‑1 (MQ=60)
                                                        AGAGTTATCGACTGAACGAAGCCGATTTTCGTGGTGAGCGCTTTGCCGACTGGCCATGCGACCTCAAAGGCAACAACGACCTGCTGGTACTCAG                                       <  SRR4302543.6606956/94‑1 (MQ=60)
                                                        AGAGTTATCGACTGAACGAAGCCGATTTTCGTGGTGAGCGCTTTGCCGACTGGCCATGCGACCTCAAAGGCAACAACGACCTGCTGGTACTCAG                                       <  SRR4302543.7531047/94‑1 (MQ=60)
                                                        AGAGTTATCGACTGAACGAAGCCGATTTTCGTGGTGAGCGCTTTGCCGACTGGCCATGCGACCTCAAAGGCAACAACGACCTGCTGGTACTCAG                                       <  SRR4302543.1313214/94‑1 (MQ=60)
                                                         GAGTTATCGACTGAACGAAGCCGATTTTCGTGGTGAGCGCTTTGCCGACTGGCCATGCGACCTCAAAGGCAACAACGACCTGCTGGTACTCAGT                                      <  SRR4302543.4726184/94‑1 (MQ=60)
                                                           GTTATCGACTGAACGAAGCCGATTTTCGTGGTGAGCGCTTTGCCGACTGGCCATGCGACCTCAAAGGCAACAACGACCTGCTGGTACTCAGTAA                                    >  SRR4302543.1031019/1‑94 (MQ=60)
                                                           GTTATCGACTGAACGAAGCCGATTTTCGTGGTGAGCGCTTTGCCGACTGGCCATGCGACCTCAAAGGCAACAACGACCTGCTGGTACTCAGTAA                                    <  SRR4302543.2088018/94‑1 (MQ=60)
                                                           GTTATCGACTGAACGAAGCCGATTTTCGTGGTGAGCGCTTTGCCGACTGGCCATGCGACCTCAAAGGCAACAACGACCTGCTGGTACTCAGTAA                                    <  SRR4302543.1347875/94‑1 (MQ=60)
                                                           GTTATCGACTGAACGAAGCCGATTTTCGTGGTGAGCGCTTTGCCGACTGGCCATGCGACCTCAAAGGCAACAACGACCTGCTGGTACTCAGTAA                                    <  SRR4302543.4430814/94‑1 (MQ=60)
                                                            TTATCGACTGAACGAAGCCGATTTTCGTGGTGAGCGCTTTGCCGACTGGCCATGCGACCTCAAAGGCAACAACGACCTGCTGGTACTCAGTAAA                                   <  SRR4302543.26428/94‑1 (MQ=60)
                                                             TATCGACTGAACGAAGCCGATTTTCGTGGTGAGCGCTTTGCCGACTGGCCATGCGACCTCAAAGGCAACAACGACCTGCTGGTACTCAGTAAAC                                  >  SRR4302543.7985242/1‑94 (MQ=60)
                                                              ATCGACTGAACGAAGCCGATTTTCGTGGTGAGCGCTTTGCCGACTGGCCATGCGACCTCAAAGGCAACAACGACCTGCTGGTACTCAGTAAACC                                 <  SRR4302543.2282174/94‑1 (MQ=60)
                                                              ATCGACTGAACGAAGCCGATTTTCGTGGTGAGCGCTTTGCCGACTGGCCATGCGACCTCAAAGGCAACAACGACCTGCTGGTACTCAGTAAACC                                 <  SRR4302543.6931477/94‑1 (MQ=60)
                                                               TCGACTGAACGAAGCCGATTTTCGTGGTGAGCGCTTTGCCGACTGGCCATGCGACCTCAAAGGCAACAACGACCTGCTGGTACTCAGTAAACCG                                <  SRR4302543.4870073/94‑1 (MQ=60)
                                                               TCGACTGAACGAAGCCGATTTTCGTGGTGAGCGCTTTGCCGACTGGCCATGCGACCTCAAAGGCAACAACGACCTGCTGGTACTCAGTAAACCG                                <  SRR4302543.4754196/94‑1 (MQ=60)
                                                               TCGACTGAACGAAGCCGATTTTCGTGGTGAGCGCTTTGCCGACTGGCCATGCGACCTCAAAGGCAACAACGACCTGCTGGTACTCAGTAAACCG                                <  SRR4302543.1219338/94‑1 (MQ=60)
                                                                CGACTGAACGAAGCCGATTTTCGTGGTGAGCGCTTTGCCGACTGGCCATGCGACCTCAAAGGCAACAACGACCTGCTGGTACTCAGTAAACCGG                               >  SRR4302543.5447099/1‑94 (MQ=60)
                                                                 GACTGAACGAAGCCGATTTTCGTGGTGAGCGCTTTGCCGACTGGCCATGCGACCTCAAAGGCAACAACGACCTGCTGGTACTCAGTAAACCGGA                              >  SRR4302543.1325892/1‑94 (MQ=60)
                                                                  ACTGAACGAAGCCGATTTTCGTGGTGAGCGCTTTGCCGACTGGCCATGCGACCTCAAAGGCAACAACGACCTGCTGGTACTCAGTAAACCGGAA                             <  SRR4302543.2444763/94‑1 (MQ=60)
                                                                  ACTGAACGAAGCCGATTTTCGTGGTGAGCGCTTTGCCGACTGGCCATGCGACCTCAAAGGCAACAACGACCTGCTGGTACTCAGTAAACCGGAA                             >  SRR4302543.423176/1‑94 (MQ=60)
                                                                   CTGAACGNAGCCGATTTTCGTGGTGAACGCTTTGCCGACTGGCCATGCGACCTCAAAGGCAACAACGACCTGCTGGTACTCAGTAAACCGGAAG                            >  SRR4302543.5275724/1‑94 (MQ=60)
                                                                   CTGAACGAAGCCGATTTTCGTGGTGAGCGCTTTGCCGACTGGCCATGCGACCTCAAAGGCAACAACGACCTGCTGGTACTCAGTAAACCGGAAG                            >  SRR4302543.37023/1‑94 (MQ=60)
                                                                   CTGAACGAAGCCGATTTTCGTGGTGAGCGCTTTGCCGACTGGCCATGCGACCTCAAAGGCAACAACGACCTGCTGGTACTCAGTAAACCGGAAG                            <  SRR4302543.2478239/94‑1 (MQ=60)
                                                                    TGAACGAAGCCGATTTTCGTGGTGAGCGCTTTGCCGACTGGCCATGCGACCTCAAAGGCAACAACGACCTGCTGGTACTCAGTAAACCGGAAGT                           >  SRR4302543.7197017/1‑94 (MQ=60)
                                                                    TGAACGAAGCCGATTTTCGTGGTGAGCGCTTTGCCGACTGGCCATGCGACCTCAAAGGCAACAACGACCTGCTGGTACTCAGTAAACCGGAAGT                           >  SRR4302543.3905792/1‑94 (MQ=60)
                                                                    TGAACGAAGCCGATTTTCGTGGTGAGCGCTTTGCCGACTGGCCATGCGACCTCAAAGGCAACAACGACCTGCTGGTACTCAGTAAACCGGAAGT                           >  SRR4302543.3905791/1‑94 (MQ=60)
                                                                    TGAACGAAGCCGATTTTCGTGGTGAGCGCTTTGCCGACTGGCCATGCGACCTCAAAGGCAACAACGACCTGCTGGTACTCAGTAAACCGGAAGT                           <  SRR4302543.6662212/94‑1 (MQ=60)
                                                                      AACGAAGCCGATTTTCGTGGTGAGCGCTTTGCCGACTGGCCATGCGACCTCAAAGGCAACAACGACCTGCTGGTACTCAGTAAACCGGAAGTGG                         >  SRR4302543.5201359/1‑94 (MQ=60)
                                                                      AACGAAGCCGATTTTCGTGGTGAGCGCTTTGCCGACTGGCCATGCGACCTCAAAGGCAACAACGACCTGCTGGTACTCAGTAAACCGGAAGTGA                         <  SRR4302543.2464700/94‑1 (MQ=60)
                                                                      AACGAAGCCGATTTTCGTGGTGAGCGCTTTGCCGACTGGCCATGCGACCTCAAAGGCAACAACGACCTGCTGGTACTCAGTAAACCGGAAGTGA                         >  SRR4302543.7295059/1‑94 (MQ=60)
                                                                       ACGAAGCCGATTTTCGTGGTGAGCGCTTTGCCGACTGGCCATGCGACCTCAAAGGCAACAACGACCTGCTGGTACTCAGTAAACCGGAAGTGAT                        <  SRR4302543.1796646/94‑1 (MQ=60)
                                                                       ACGAAGCCGATTTTCGTGGTGAGCGCTTTGCCGACTGGCCATGCGACCTCAAAGGCAACAACGACCTGCTGGTACTCAGTAAACCGGAAGTGAT                        <  SRR4302543.5997759/94‑1 (MQ=60)
                                                                        CGAAGCCGATTTTCGTGGTGAGCGCTTTGCCGACTGGCCATGCGACCTCAAAGGCAACAACGACCTGCTGGTACTCAGTAAACCGGAAGTGATC                       <  SRR4302543.5940962/94‑1 (MQ=60)
                                                                        CGAAGCCGATTTTCGTGGTGAGCGCTTTGCCGACTGGCCATGCGACCTCAAAGGCAACAACGACCTGCTGGTACTCAGTAAACCGGAAGTGATC                       <  SRR4302543.5000329/94‑1 (MQ=60)
                                                                         GAAGCCGATTTTCGTGGTGAGCGCTTTGCCGACTGGCCATGCGACCTCAAAGGCAACAACGACCTGCTGGTACTCAGTAAACCGGAAGTGATCG                      <  SRR4302543.379236/94‑1 (MQ=60)
                                                                         GAAGCCGATTTTCGTGGTGAGCGCTTTGCCGACTGGCCATGCGACCTCAAAGGCAACAACGACCTGCTGGTACTCAGTAAACCGGAAGTGATCG                      <  SRR4302543.1942780/94‑1 (MQ=60)
                                                                         GAAGCCGATTTTCGTGGTGAGCGCTTTGCCGACTGGCCATGCGACCTCAAAGGCAACAACGACCTGCTGGTACTCAGTAAACCGGAAGTGATCG                      <  SRR4302543.3099537/94‑1 (MQ=60)
                                                                         GAAGCCGATTTTCGTGGTGAGCGCTTTGCCGACTGGCCATGCGACCTCAAAGGCAACAACGACCTGCTGGTACTCAGTAAACCGGAAGTGATCG                      <  SRR4302543.2591828/94‑1 (MQ=60)
                                                                          AAGCCGATTTTCGTGGTGAGCGCTTTGCCGACTGGCCATGCGACCTCAAAGGCAACAACGACCTGCTGGTACTCAGTAAACCGGAAGTGATCGC                     <  SRR4302543.1449224/94‑1 (MQ=60)
                                                                          AAGCCGATTTTCGTGGTGAGCGCTTTGCCGACTGGCCATGCGACCTCAAAGGCAACAACGACCTGCTGGTACTCAGTAAACCGGAAGTGATCGC                     <  SRR4302543.1128201/94‑1 (MQ=60)
                                                                           AGCCGATTTTCGTGGTGAGCGCTTTGCCGACTGGCCATGCGACCTCAAAGGCAACAACGACCTGCTGGTACTCAGTAAACCGGAAGTGATCGCC                    <  SRR4302543.3840307/94‑1 (MQ=60)
                                                                           AGCCGATTTTCGTGGTGAGCGCTTTGCCGACTGGCCATGCGACCTCAAAGGCAACAACGACCTGCTGGTACTCAGTAAACCGGAAGTGATCGCC                    <  SRR4302543.3840306/94‑1 (MQ=60)
                                                                           AGCCGATTTTCGTGGTGAGCGCTTTGCCGACTGGCCATGCGACCTCAAAGGCAACAACGACCTGCTGGTACTCAGTAAACCGGAAGTGATCGCC                    <  SRR4302543.6984917/94‑1 (MQ=60)
                                                                            GCCGATTTTCGTGGTGAGCGCTTTGCCGACTGGCCATGCGACCTCAAAGGCAACAACGACCTGCTGGTACTCAGTAAACCGGAAGTGATCGCCG                   <  SRR4302543.2924422/94‑1 (MQ=60)
                                                                             CCGATTTTCGTGGTGAGCGCTTTGCCGACTGGCCATGCGACCTCAAAGGCAACAACGACCTGCTGGTACTCAGTAAACCGGAAGTGATCGCCGC                  >  SRR4302543.7039614/1‑94 (MQ=60)
                                                                              CGATTTTCGTGGTGAGCGCTTTGCCGACTGGCCATGCGACCTCAAAGGCAACAATGACCTGCTGGTACTCAGTAAACCGGAAGTGATCGCCGCT                 >  SRR4302543.7054282/1‑94 (MQ=60)
                                                                              CGATTTTCGTGGTGAGCGCTTTGCCGACTGGCCATGCGACCTCAAAGGCAACAACGACCTGCTGGTACTCAGTAAACCGGAAGTGATCGCCGCT                 >  SRR4302543.7843908/1‑94 (MQ=60)
                                                                              CGATTTGCGTGGTGAGCGCTTTGCCGACTGGCCATGCGACCTCAAAGGCAACAACGACCTGCTGGTACTCAGTAAACCGGAAGTGATCGCCGCT                 <  SRR4302543.1847525/94‑1 (MQ=60)
                                                                               GATTTTCGTGGTGAGCGCTTTGCCGACTGGCCATGCGACCTCAAAGGCAACAACGACCTGCTGGTACTCAGTAAACCGGAAGTGATCGCCGCTA                >  SRR4302543.1007260/1‑94 (MQ=60)
                                                                                  TTTCGTGGTGAGCGCTTTGCCGACTGGCCATGCGACCTCAAAGGCAACAACGACCTGCTGGTACTCAGTAAACCGGAAGTGATCGCCGCTATCC             <  SRR4302543.1107784/94‑1 (MQ=60)
                                                                                  TTTCGTGGTGAGCGCTTTGCCGACTGGCCATGCGACCTCAAAGGCAACAACGACCTGCTGGTACTCAGTAAACCGGAAGTGATCGCCGCTATCC             >  SRR4302543.7277532/1‑94 (MQ=60)
                                                                                  TTTCGTGGTGAGCGCTTTGCCGACTGGCCATGCGACCTCAAAGGCAACAACGACCTGCTGGTACTCAGTAAACCGGAAGTGATCGCCGCTATCC             >  SRR4302543.4050527/1‑94 (MQ=60)
                                                                                  TTTCGTGGTGAGCGCTTTGCCGACTGGCCATGCGACCTCAAAGGCAACAACGACCTGCTGGCACTCAGTAAAACGGAAGTGATCGCCGCTATCC             >  SRR4302543.7277533/1‑94 (MQ=60)
                                                                                  TTTCGTGGTGAGCGCTGTGCCGACTGGCCATGCGACCTCAAAGGCAACAACGACCTGCTGGTACTCAGTAAACCGGAAGTGATCGCCGCTATCC             <  SRR4302543.6533086/94‑1 (MQ=60)
                                                                                   TTCGTGGTGAGCGCTTTGCCGACTGGCCATGCGACCTCAAAGGCAACAACGACCTGCTGGTACTCAGTAAACCGGAAGTGATCGCCGCTATCCA            <  SRR4302543.2255603/94‑1 (MQ=60)
                                                                                     CGTGGTGAGCGCTTTGCCGACTGGCCATGCGACCTCAAAGGCAACAACGACCTGCTGGTACTCAGTAAACCGGAAGTGATCGCCGCTATCCACA          >  SRR4302543.5177739/1‑94 (MQ=60)
                                                                                     CGTGGTGAGCGCTTTGCCGACTGGCCATGCGACCTCAAAGGCAACAACGACCTGCTGGTACTCAGTAAACCGGAAGTGATCGCCGCTATCCACA          <  SRR4302543.4560805/94‑1 (MQ=60)
                                                                                     CGTGGTGAGCGCTTTGCCGACTGGCCATGCGACCTCAAAGGCAACAACGACCTGCTGGTACTCAGTAAACCGGAAGTGATCGCCGCTATCCACA          <  SRR4302543.3473833/94‑1 (MQ=60)
                                                                                       TGGTGAGCGCTTTGCCGACTGGCCATGCGACCTCAAAGGCAACAACGACCTGCTGGTACTCAGTAAACCGGAAGTGATCGCCGCTATCCACAAC        >  SRR4302543.6997751/1‑94 (MQ=60)
                                                                                       TGGTGAGCGCTTTGCCGACTGGCCATGCGACCTCAAAGGCAACAACGACCTGCTGGTACTCAGTAAACCGGAAGTGATCGCCGCTATCCACAAC        >  SRR4302543.5868571/1‑94 (MQ=60)
                                                                                       TGGTGAGCGCTTTGCCGACTGGCCATGCGACCTCAAAGGCAACAACGACCTGCTGGTACTCAGTAAACCGGAAGTGATCGCCGCTATCCACAAC        <  SRR4302543.4081960/94‑1 (MQ=60)
                                                                                       TGGTGAGCGCTTTGCCGACTGGCCATGCGACCTCAAAGGCAACAACGACCTGCTGGTACTCAGTAAACCGGAAGTGATCGCCGCTATCCACAAC        >  SRR4302543.6320088/1‑94 (MQ=60)
                                                                                       TGGTGAGCGCTTTGCCGACTGGCCATGCGACCTCAAAGGCAACAACGACCTGCTGGTACTCAGTAAACCGGAAGTGATCGCCGCTATCCACAAC        <  SRR4302543.4799079/94‑1 (MQ=60)
                                                                                       TGGTGAGCGCTTTGCCGACTGGCCATGCGACCTCAAAGGCAACAACGACCTGCTGGTACTCAGTAAACCGGAAGTGATCGCCGCTATCCACAAC        <  SRR4302543.6996964/94‑1 (MQ=60)
                                                                                       TGGTGAGCGCTTTGCCGACTGGCCATGCGACCTCAAAGGCAACAACGACCTGCTGGTACTCAGGACACCGGAAGCGATCGCCGCTATCCACAAC        >  SRR4302543.6997750/1‑94 (MQ=60)
                                                                                       GGGTGAGCGCTTTGCCGGCTGGCCATGCGTCCTCAAAGGCAACAACGACCTGCTTGTACCCGGTAAACCGGAAGTGATCGCCGCTATCCACAAC        <  SRR4302543.4081961/94‑1 (MQ=60)
                                                                                       GGGTGAGCGCTTTGCCGACTGGCCATGCCACCTCAAAGGCAACAACGACCTGCTGGTACTCAGTAAACCGGAAGTGATCGCCGCTATCCACAAC        <  SRR4302543.7678528/94‑1 (MQ=60)
                                                                                        GGTGAGCGCTTTGCCGCCTGGCCATGCGACCTCAAAGGCAACAACGACCTGCTGGTACTCAGTAAACCGGAAGTGATCGCCGCTATCCACAACG       <  SRR4302543.7071492/94‑1 (MQ=60)
                                                                                        GGTGAGCGCTTTGCCGACTGGCCATGCGACCTCAAAGGCAACAACGACCTGCTGGTACTCAGTAAACCGGAAGTGATCGCCGCTATCCACAACC       >  SRR4302543.1660857/1‑94 (MQ=60)
                                                                                        GGTGAGCGCTTTGCCGACTAGCCATGCGACCTCAAAGGCAACAACGACCTGCTGGTACTCAGTAAACCGGAAGTGATCGCCGCTATCCACAACG       <  SRR4302543.7767306/94‑1 (MQ=60)
                                                                                         GTGAGCGCTTTGCCGACTGGCCATGCGACCTCAAAGGCAACAACGACCTGCTGGTACTCAGTAAACCGGAAGTGATCGCCGCTATCCACAACGC      >  SRR4302543.4245559/1‑94 (MQ=60)
                                                                                          TGAGCGCTTTGCCGACTGGCCATGCGACCTCAAAGGCAACAACGACCTGCTGGTACTCAGTAAACCGGAAGTGATCGCCGCTATCCACAACGCC     >  SRR4302543.2340537/1‑94 (MQ=60)
                                                                                            AGCGCTTTGCCGACTGGCCATGCGACCTCAAAGGCAACAACGACCTGCTGGTACTCAGTAAACCGGAAGTGATCGCCGCTATCCACAACGCCTA   <  SRR4302543.5665213/94‑1 (MQ=60)
                                                                                            AGCGCTTTGCCGACTGGCCATGCGACCTCAAAGGCAACAACGACCTGCTGGTACTCAGTAAACCGGAAGTGATCGCCGCTATCCACAACGCCTA   >  SRR4302543.6038127/1‑94 (MQ=60)
                                                                                             GCGCTTTGCCGACTGGCCATGCGACCTCAAAGGCAACAACGACCTGCTGGTACTCAGTAAACCGGAAGTGATCGCCGCTATCCACAACGCCTAC  <  SRR4302543.2752461/94‑1 (MQ=60)
                                                                                             |                                                                                             
TGCGCAGTTAAATGAACGTATTCTGGTGCTGGACGGCGGTATGGGCACCATGATCCAGAGTTATCGACTGAACGAAGCCGATTTTCGTGGTGAACGCTTTGCCGACTGGCCATGCGACCTCAAAGGCAACAACGACCTGCTGGTACTCAGTAAACCGGAAGTGATCGCCGCTATCCACAACGCCTAC  >  NC_000913/4221433‑4221619

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 10 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑