Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I1 R1	1696	279.3	15874187	97.8%	15524954	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,225,139	T→C	intergenic (+49/‑171)	metH → / → yjbB	B12‑dependent homocysteine‑N5‑methyltetrahydrofolate transmethylase/putative alpha helix protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,225,139	0	T	C	98.5%	614.5 / NA	208	intergenic (+49/‑171)	metH/yjbB	B12‑dependent homocysteine‑N5‑methyltetrahydrofolate transmethylase/putative alpha helix protein
Reads supporting (aligned to +/- strand): ref base T (1/0); major base C (105/97); minor base G (3/0); total (111/97)
Fisher's exact test for biased strand distribution p-value = 2.48e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 251 total aligned reads displayed.

GCGCTGGCTGGCACCGAATCTGGGGTATGACGCGGACTGATTCACAAATCTGTCACTTTTCCTTACAACAAACAGGGCGCTCAATGAGTGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAACAGGCTGGAATAATCTTAGCCGGAAAAACGAATGTCAAAAGGCACAGT  >  NC_000913/4225051‑4225227

  gctggctgGCACCGAATCTGGGGTATGACGCGGACTGATTCACAAATCTGTCACTTTTCCTTACAACAAACAGGGCGCTCAATGAGCGCCCTg                                                                                    >  1:2248352/1‑93 (MQ=255)

    tggctggcACCGAATCTGGGGTATGACGCGGACTGATTCACAAATCTGTCACTTTTCCTTACAACAAACAGGGCGCTCAATGAGCGCCCTGtc                                                                                  >  2:6346998/1‑93 (MQ=255)

    tggctggcACCGAATCTGGGGTATGACGCGGACTGATTCACAAATCTGTCACTTTTCCTTACAACAAACAGGGCGCTCAATGAGCGCCCTGtc                                                                                  <  2:3842481/93‑1 (MQ=255)

    tggctggcACCGAATCTGGGGTATGACGCGGACTGATTCACAAATCTGTCACTTTTCCTTACAACAAACAGGGCGCTCAATGAGCGCCCTGtc                                                                                  <  2:609541/93‑1 (MQ=255)

     ggctggcACCGAATCTGGGGTATGACGCGGACTGATTCACAAATCTGTCACTTTTCCTTACAACAAACAGGGCGCTCAATGAGCGCCCTGtct                                                                                 >  2:6698116/1‑93 (MQ=255)

          gcACCGAATCTGGGGTATGACGCGGACTGATTCACAAATCTGTCACTTTTCCTTACAACAAACAGGGCGCTCAATGAGCGCCCTGTCTCTtta                                                                            <  1:5177214/93‑1 (MQ=255)

          gcACCGAATCTGGGGTATGACGCGGACTGATTCACAAATCTGTCACTTTTCCTTACAACAAACAGGGCGCTCAATGAGCGCCCTGTCTCTtta                                                                            <  1:2503491/93‑1 (MQ=255)

           cACCGAATCTGGGTTATGACGCGGACTGATTCACAAATCTGTCACTTTTCCTTACAACAAACAGGGCGCTCAATGAGCGCCCTGTCTCTttat                                                                           <  1:1287193/93‑1 (MQ=255)

           cACCGAATCTGGGGTATGACGCGGACTGATTCACAAATCTGTCACTTTTCCTTACAACAAACAGGGCGCTCAATGAGCGCCCTGTCTCTttat                                                                           >  2:6401926/1‑93 (MQ=255)

            aCCGAATCTGGGGTATGACGCGGACTGATTCACAAATCTGTCACTTTTCCTTACAACAAACAGGGCGCTCAATGAGCGCCCTGTCTCTttatt                                                                          <  1:914555/93‑1 (MQ=255)

            aCCGAATCTGGGGTATGACGCGGACTGATTCACAAATCTGTCACTTTTCCTTACAACAAACAGGGCGCTCAATGAGCGCCCCGTCTCTttatt                                                                          >  2:4768060/1‑93 (MQ=255)

            aCCGAATCTGGGGTATGACGCGGACTGATTCACAAATCTGTCACTTTTCCTTACAACAAACAGCGCGCTCAATGAGCGCCCTGTCTCTttatt                                                                          >  1:6724886/1‑93 (MQ=255)

             ccGAATCTGGGGTATGACGCGGACTGATTCACAAATCTGTCACTTTTCCTTACAACAAACAGGGCGCTCAATGAGCGCCCTGTCTCTttatta                                                                         >  2:6022098/1‑93 (MQ=255)

             ccGAATCTGGGGTATGACGCGGACTGATTCACAAATCTGTCACTTTTCCTTACAACAAACAGGGCGCTCAATGAGCGCCCTGTCTCTttatta                                                                         <  1:6708746/93‑1 (MQ=255)

               gAATCTGGGGTATGACGCGGACTGATTCACAAATCTGTCCCTTTTCCTTACAACAAACAGGGCGCTCAATGAGCCCCCTGTCTCTTTATTAat                                                                       <  1:2467434/93‑1 (MQ=255)

               gAATCTGGGGTATGACGCGGACTGATTCACAAATCTGTCACTTTTCCTTACAACAAACAGGGCGCTCAATGAGCGCCCTGTCTCTTTATTAat                                                                       >  2:3420907/1‑93 (MQ=255)

                 aTCTGGGGTATGACGCGGACTGATTCACAAATCTGTCACTTTTCCTTACAACAAACAGGGCGCTCCATGAGGGCCCTGTCTCTTTTTTTatat                                                                     >  1:5595396/1‑93 (MQ=255)

                  tCTGGGGTATGACGCGGACTGATTCACAAATCTGTCACTTTTCCTTACAACAAACAGGGCGCTCCATGAGCGCCCCGTCTCTTTTTTTatatt                                                                    >  1:2888636/1‑92 (MQ=255)

                  tCTGGGGTATGACGCGGACTGATTCACAAATCTGTCACTTTTCCTTACAACAAACAGGGCGCTCAATGAGCGCCCTGTCTCTTTATTAATATg                                                                    >  1:2048460/1‑93 (MQ=255)

                   cTGGGGTATGACGCGGACTGATTCACAAATCTGTCACTTTTCCTTACAACAAACAGGGCGCTCAATGAGCGCCCTGTCTCTTTATTAATATGa                                                                   >  1:4659684/1‑93 (MQ=255)

                    tGGGGTATGACGCGGACTGATTCACAAATCTGTCACTTTTCATTACAACAAACAGGGCGCTCAATGAGCGCCCTGTCTCTTTATTAATATGaa                                                                  <  2:1235812/93‑1 (MQ=255)

                     ggggTATGACGCGGACTGATTCACAAATCTGTCACTTTTCCTTACAACAACCAGGGCGCTCAATGAGCGCCCTGTCTCTTTATTAATATGaaa                                                                 <  2:2510444/93‑1 (MQ=255)

                      gggTATGACGCGGACTGATTCACAAATCTGTCACTTTTCCTTACAACAAACAGGGCGCTCAATGAGCGCCCTGTCTCTTTATTAATATGAAac                                                                <  1:4121732/93‑1 (MQ=255)

                      gggTATGACGCGGACTGATTCACAAATCTGTCACTTTTCCTTACAACAAACAGGGCGCTCAATGAGCGCCCTGTCTCTTTATTAATATGAAac                                                                <  1:6131674/93‑1 (MQ=255)

                        gTATGACGCGGACTGATTCACAAATCTGTCACTTTTCCTTACAACAAACAGGGCGCTCAATGAGCGCCCTGTCTCTTTATTAATATGAAacac                                                              >  2:4458299/1‑93 (MQ=255)

                           tGACGCGGACTGATTCACAAATCTGTCACTTTTCCTTACAACAAACAGGGCGCTCAATGAGCGCCCTGTCTCTTTATTAATATGAAACACTta                                                           <  2:5221324/93‑1 (MQ=255)

                           tGACGCGGACTGATTCACAAATCTGTCACTTTTCCTTACAACAAACAGGGCGCTCAATGAGCGCCCTGTCTCTTTATTAATATGAAACACTta                                                           >  2:2635455/1‑93 (MQ=255)

                           tGACGCGGACTGATTCACAAATCTGTCACTTTTCCTTACAACAAACAGGGCGATCGATTATCCGCCTACTTCTTTTTTAATATTAAACACttt                                                           >  1:7193128/1‑92 (MQ=255)

                           tGACGCGGACTGATTCACAAATCTGTCACTCTTCCTTACAACAAACAGGGCGCTCAATGAGCGCCCTGTCTCTTTATTAATATGAAACACTta                                                           <  2:7731163/93‑1 (MQ=255)

                             aCGCGGACTGATTCACAAATCTGTCACTTTTCCTTACAACAAACAGGGCGCTCAATGAGCGCCCTGTCTCTTTATTAATATGAAACACTtata                                                         <  1:2654569/93‑1 (MQ=255)

                             aCGCGGACTGATTCACAAATCTGTCACTTTTCCTTACAACAAACAGGGCGCTCAATGAGCGCCCTGTCTCTTTATTAATATGAAACACTtata                                                         <  2:3787524/93‑1 (MQ=255)

                             aCGCGGACTGATTCACAAATCTGTCACTTTTCCTTACAACAAACAGGGCGATCAATAAGCGCCCTGTCTCTTTATTAATATGAAACACTtata                                                         <  1:4944504/93‑1 (MQ=255)

                               gcgGACTGATTCACAAATCTGTCACTTTTCCTTACAACAAACAGGGCGCTCAATGAGCGCCCTGTCTCTTTATTAATATGAAACACTTATACt                                                       >  2:3883726/1‑93 (MQ=255)

                                cgGACTGATTCACAAACCTGTCACTTTTCCTTACAACAAACAGGGCGCTAAATGAGCGCCCTGTCTCTTTATTAATATGAAACACTTATACTg                                                      <  2:6532750/93‑1 (MQ=255)

                                  gACTGATTCACAAATCTGTCACTTTTCCTTACAACAAACAGGGCGCTCAATGAGCGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGa                                                    >  1:7499019/1‑93 (MQ=255)

                                  gACTGATTCACAAATCTGTCACTTTTCCTTACAACAAACAGGGCGCTCAATGAGCGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGa                                                    >  1:6529968/1‑93 (MQ=255)

                                  gACTGATTCACAAATCTGTCACTTTTCCTTACAACAAACAGGGCGCTCAATGAGCGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGa                                                    >  1:6188471/1‑93 (MQ=255)

                                  gACTGATTCACAAATCTGTCACTTTTCCTTACAACAAACAGGGCGCTCAATGAGCGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGa                                                    >  1:542557/1‑93 (MQ=255)

                                  gACTGATTCACAAATCTGTCACTTTTCCTTACAACAAACAGGGCGCTCAATGAGCGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGa                                                    >  1:4596210/1‑93 (MQ=255)

                                   aCTGATTCACAAATCTGTCACTTTTCCTTACAACAAACAGGGCGCTCAATGAGCGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGaa                                                   >  2:5488445/1‑93 (MQ=255)

                                      gATTCACAAATCTGTCACTTTTCCTTACAACAAACAGGGCGCTCAATGAGCGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAACa                                                <  1:7632878/93‑1 (MQ=255)

                                      gATTCACAAATCTGTCACTTTTCCTTACAACAAACAGGGCGCTCAATGAGCGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAACa                                                <  2:4065385/93‑1 (MQ=255)

                                      gATTCACAAATCTGTCACTTTTCCTAACAACAAACGGGGCGCTCAATGGGCGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAACa                                                <  1:2140840/93‑1 (MQ=255)

                                       aTTCACAAATCTGTCACTTTTCCTTACAACAAACAGGGCGCTCAATGAGCGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAACAg                                               <  2:7746336/93‑1 (MQ=255)

                                       aTTCACAAATCTGTCACTTTTCCTTACAACAAACAGGGCGCTCAATGAGCGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAACAg                                               >  1:4908045/1‑93 (MQ=255)

                                       aTTCACAAATCTGTCACTTTTCCTTACAACAAACAGGGCGCTCAATGAGCGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAACAg                                               <  2:4984439/93‑1 (MQ=255)

                                        ttCACAAATCTGTCACTTTTCCTTACAACAAACAGGGCGCTCAATGAGCGCCCTGTCTCTTTATTTATATGAAACACTTATACTGGAAACAgg                                              >  1:7143599/1‑93 (MQ=255)

                                        ttCACAAATCTGTCACTTTTCCTTACAACAAACAGGGCGCTCAATGAGCGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAACAgg                                              <  1:3668784/93‑1 (MQ=255)

                                         tCACAAATCTGTCACTTTTCCTTACAACAAACAGGGCGCTCAATGAGCGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAACAGGc                                             <  2:1040419/93‑1 (MQ=255)

                                            caAATCTGTCACTTTTCCTTACAACAAACAGGGCGCTCAATGAGCGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAACAGGCTgg                                          >  2:428676/1‑93 (MQ=255)

                                             aaaTCTGTCACTTTTCCTTACAACAAACAGGGCGCTCAATGAGCGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAACAGGCTGGa                                         >  2:5359030/1‑93 (MQ=255)

                                               aTCTGTCACTTTTCCTTACAACAAACAGGGCGCTCAATGAGCGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAACAGGCTGGaat                                       >  1:4054261/1‑93 (MQ=255)

                                               aTCTCTCACTTCTGCTAAAACCAAGCAGGGCCCTCAATGAGCCCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAACAGGCTGGaat                                       <  1:3479222/93‑1 (MQ=255)

                                                 cTGTCTTTTTTCCTAACAAAAAACGGGGCGCTCAAGGAGCCCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAACAGGCTGGaataa                                     <  2:7162620/93‑1 (MQ=255)

                                                 cTGTCACTTTTCCTTACAACAAACAGGGCGCTCAATGAGCGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAACAGGCTGGaataa                                     <  2:7283908/93‑1 (MQ=255)

                                                   gTCACTTTTCCTTACAACAAACAGGGCGCTCAATGAGCGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAACAGGCTGGAATAATc                                   >  1:1101997/1‑93 (MQ=255)

                                                    tcttttttCCTAACAAAAAGCGGGGCGCTCAATGAGCGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAACAGGCTGGAATAATCt                                  <  2:1699379/89‑1 (MQ=255)

                                                    tCACTTTTCCTTACAACAAACAGGGCGCTCAATGAGCGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAACAGGCTGGAATAATCt                                  <  2:4809095/93‑1 (MQ=255)

                                                    tCACTTTTCCTTACAACAAACAGGGCGCTCAATGAGCGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAACAGGCTGGAATAATCt                                  <  2:6841361/93‑1 (MQ=255)

                                                    tCACTTTTCCTTACAACAAACAGGGCGCTCAATGAGCGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAACAGGCTGGAATAATCt                                  <  1:7731875/93‑1 (MQ=255)

                                                     ccttttcCCTTACAAAAACCGGGGCGCTGAAGGAGCCCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAACAGGCTGGAATAATCtt                                 <  2:5836245/90‑1 (MQ=255)

                                                     cACTTTCCCTTACAACAAACAGGGCGCTCAATGAGCCCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAACAGGCTGGAATAATCtt                                 <  2:2402065/93‑1 (MQ=255)

                                                      aCTTTTCCTTACAACAAACAGGGCGCTCAATGAGCGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAACAGGCTGGAATAATCTTa                                >  1:3245309/1‑93 (MQ=255)

                                                      aCTTTTCCTTACAACAAACAGGGCGCTCAATGAGCGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAACAGGCTGGAATAATCTTa                                <  2:2684442/93‑1 (MQ=255)

                                                       tttttCCACACAACAAGCAGGGCCCTAAATGGCCGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAACAGGCTGGAATAATCTTAg                               <  1:6940003/92‑1 (MQ=255)

                                                       cTTTTCCTTACAACAAACAGGGCGCTAAATGAGCCCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAACAGGCTGGAATAATCTTAg                               <  2:7424147/93‑1 (MQ=255)

                                                        ttttCCTTACAACAAACAGGGCGCTCAATGAGCGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAACAGGCTGGAATAATCTTAGc                              >  1:3494163/1‑93 (MQ=255)

                                                          ttCCTTACAACAAACAGGGCGCTCAATGAGCGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAACAGGCTGGAATAATCTTAGCCg                            >  2:894201/1‑93 (MQ=255)

                                                           tCCTTACAACAAACAGGGCGCTCAATGAGCGCCCCGTCTCTTTTTTTATATGAAACACTTATACTGGAAACAGGCTGGAATAATCTTAGCCgg                           >  2:1787443/1‑93 (MQ=255)

                                                            ccTTACAACAAACAGGGCGCTCAATGAGCGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAACCGGCTGGAATAATCTTAGCCGGa                          >  2:2569314/1‑93 (MQ=255)

                                                            ccTTACAACAAACAGGGCGCTCAATGAGCGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAACAGGCTGGAATAATCTTAGCCGGa                          >  1:2069910/1‑93 (MQ=255)

                                                            ccTTACAAAAAACAGGCCCGCAAATGAGCGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAACAGGCTGGAATAATCTTAGCCGGa                          <  2:3402703/93‑1 (MQ=255)

                                                              ttACAACAAACAGGGCGCTCAATGAGCGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAACAGGCTGGAATAATCTTAGCCGGaaa                        >  1:659554/1‑93 (MQ=255)

                                                               taaaacaaacaGGGCGCTAAAGGAGCCCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAACAGGCTGGAATAATCTTAGCCGGaaaa                       <  1:1477907/90‑1 (MQ=255)

                                                                acaacaAACAGGTCTTCCAATTAGCGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAACAGTCTTGAATAATCTTAGCCGGaaaaa                      >  2:7237979/1‑93 (MQ=255)

                                                                acaacaAACAGGGCGCTCAATGAGCGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAACAGGCTGGAATAATCTTAGCCGGaaaaa                      <  1:6847367/93‑1 (MQ=255)

                                                                acaacaAACAGGGCGCTCAATGAGCGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAACAGGCTGGAATAATCTTAGCCGGaaaaa                      <  1:1148214/93‑1 (MQ=255)

                                                                acaaaaAACGGGCCGCTCAATGAGCCCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAACAGGCTGGAATAATCTTAGCCGGaaaaa                      <  2:2863999/93‑1 (MQ=255)

                                                                  aacaaacaGGGCGCTCAATGAGCGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAACAGGCTGGAATAATCTTAGCCGGAAAAACg                    >  1:1006470/1‑93 (MQ=255)

                                                                   acaaacaGGGCGCTCAATGAGCGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAACAGGCTGGGATAATCTTAGCCGGAAAAAAGa                   >  2:1958464/1‑93 (MQ=255)

                                                                    caaacaGGGCTCTCAATGAGCGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAACAGGCTGGAATAATCTTAGCCGGAAAAACGaa                  <  2:2334789/93‑1 (MQ=255)

                                                                    caaacaGGGCGCTCAATGAGCGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAACAGGCTGGAATAATCTTAGCCGGAAAAACGaa                  <  1:4819770/93‑1 (MQ=255)

                                                                    caaacaGGGCGCTCAATGAGCGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAACAGGCTGGAATAATCTTAGCCGGAAAAACGaa                  <  1:3741992/93‑1 (MQ=255)

                                                                      aacaGGGCGCTCAATGAGCGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAACAGGCTGGAATAATCTTAGCCGGAAAAACGAATg                <  1:1266650/93‑1 (MQ=255)

                                                                       acaGGGCGCTCAATGAGCGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAACAGGCTGGAATAATCTTAGCCGGAAAAACGAATGt               >  2:663417/1‑93 (MQ=255)

                                                                        caGGGCGCTCAATGAGCGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAACAGGCTGGAATAATCTTAGCCGGAAAAACGAATGTc              >  2:1196270/1‑93 (MQ=255)

                                                                        caGGGCGCTCAATGAGCGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAACAGGCTGGAATAATCTTAGCCGGAAAAACGAATGTc              <  2:7899116/93‑1 (MQ=255)

                                                                          gggCGCTCAATGAGCGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAACAGGCTGGAATAATCTTAGCCGGAAAAACGAATGTCaa            >  1:2730259/1‑93 (MQ=255)

                                                                           ggCGCTCAATGAGGGCCCTGTCTCTTTTTTAATATGAAACACTTATACTGGAAACAGGCTGGGATAATCCTTTCCGGGAAAACGAAAGTCaaa           >  2:1877978/1‑93 (MQ=255)

                                                                            ggtcTCAAGGGGCGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAACAGGCTGGAATAATCTTAGCCGGAAAAACGAATGTCaaaa          <  2:3245308/90‑1 (MQ=255)

                                                                            gcgcTCAATGAGCGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAACAGGCTGGAATAATCTTAGCCGGAAAAACGAATGTCaaaa          <  2:5035075/93‑1 (MQ=255)

                                                                            gcgcTCAATGAGCGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAACAGGCTGGAATAATCTTAGCCGGAAAAACGAATGTCaaaa          <  1:586327/93‑1 (MQ=255)

                                                                            gcgcTCAATGACCCCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAACAGGCTGGAATAATCTTAGCCGGAAAAACGAATGTCaaaa          <  2:4548713/93‑1 (MQ=255)

                                                                            gcgcTCAACGACCCCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAACAGGCTGGAATAATCTTAGCCGGAAAAACGAATGTCaaaa          <  2:397698/93‑1 (MQ=255)

                                                                             ctcTCAACGAGCCCGCTGTCTCTTTATTAATATGAAACACTTATACTGGAAACAGGCTGGAATAATCTTAGCCGGAAAAACGAATGTCAAAAg         <  1:4819545/91‑1 (MQ=255)

                                                                               cTCAATGAGCGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAACAGGCTGGAATAATCTTAGCCGGAAAAACGAATGTCAAAAGGc       >  2:5470397/1‑93 (MQ=255)

                                                                                 cAATGAGCGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAACAGGCTGGAATAATCTTAGCCGGAAAAACGAATGTCAAAAGGcac     >  2:1299946/1‑93 (MQ=255)

                                                                                 cAATGAGCGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAACAGGCTGGAATAATCTTAGCCGGAAAAACGAATGTCAAAAGGcac     >  2:5785297/1‑93 (MQ=255)

                                                                                    tGAGCGCCCTGTCTCTTTATTAATATGAAACACTtata                                                         <  1:7793094/38‑1 (MQ=255)

                                                                                    tGAGCGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAACAGGCTGGAATAATCTTAGCCGGAAAAACGAATGTCAAAAGGCACAga  >  2:6432125/1‑92 (MQ=255)

GCGCTGGCTGGCACCGAATCTGGGGTATGACGCGGACTGATTCACAAATCTGTCACTTTTCCTTACAACAAACAGGGCGCTCAATGAGTGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAACAGGCTGGAATAATCTTAGCCGGAAAAACGAATGTCAAAAGGCACAGT  >  NC_000913/4225051‑4225227

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 16 ≤` `ATCG/ATCG` `< 26 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

GTTGAGCGCTGGCTGGCACCGAATCTGGGGTATGACGCGGACTGATTCACAAATCTGTCACTTTTCCTTACAACAAACAGGGCGCTCAATGAGTGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAACAGGCTGGAATAATCTTAGCCGGAAAAACGAATGTCAAAAGGCACAGTAAAGA  >  NC_000913/4225046‑4225232

GTTGGGCGCTGGCTGGCACCGAATCTGGGGTATGACGCGGACTGATTCACAAATCTGTCACTTTTCCTTACAACAAACAGGGCGCTCAATGAGC                                                                                               <  SRR4302543.4999566/94‑1 (MQ=60)

GTTGGGCGCTGGCTGGCACCGAATCTGGGGTATGACGCGGACTGATTCACAAATCTGTCACTTTTCCTTACAACAAACAGGGCGCTCAATGAGC                                                                                               >  SRR4302543.2780753/1‑94 (MQ=60)

GTTGGGCGCTGGCTGGCACCGAATCTGGGGTATGACGCGGACTGATTCACAAATCTGTCACTTTTCCTTACAACAAACAGGGCGCTCAATGAGC                                                                                               <  SRR4302543.5224673/94‑1 (MQ=60)

GTTGGGCGCTGGCTGGCACCGAATCTGGGGTATGACGCGGACTGATTCACAAATCTGTCACTTTTCCTTACAACAAACAGGGCGCTCAATGAGC                                                                                               >  SRR4302543.3348754/1‑94 (MQ=60)

 TTGGGCGCTGGGTGGCACCGAATCTGGGGTATGACGCGGACTGATTCACAAATCTGTCACTTTTCCTTACAACAAACAGGGCGCTCAATGAGCG                                                                                              <  SRR4302543.216194/94‑1 (MQ=60)

 TTGGGCGCTGGCTGGCACCGAATCTGGGGTATGACGCGGACTGATTCACAAATCTGTCACTTTTCCTTACAACAAACAGGGCGCTCAATGAGCG                                                                                              <  SRR4302543.7203464/94‑1 (MQ=60)

    GGCGCTGGCTGGCACCGAATCTGGGGTATGACGCGGACTGATTCACAAATCTGTCACTTTTCCTTACAACAAACAGGGCGCTCAATGAGCGCCC                                                                                           <  SRR4302543.618678/94‑1 (MQ=60)

     GCGCTGGCTGGCACCGAATCTGGGGTATGACGCGGACTGATTCACAAATCTGTCACTTTTCCTTACAACAAACAGGGCGCTCAATGAGCGCCCT                                                                                          <  SRR4302543.7830708/94‑1 (MQ=60)

      GGCTGGCTGGCACCGAATCTGGGGTATGCCGGGGACTGATTCACAAATCTGTCACTTTTCCTTACAACAAACAGGGCGCTCAATGAGCGCCCTG                                                                                         <  SRR4302543.2346473/94‑1 (MQ=60)

      CGCTGGCTGGCACCGAATCTGGGGTATGACGCGGACTGATTCACAAATCTGTCACTTTTCCTTACAACAAACAGGGCGCTCAATGAGCGCCCTG                                                                                         >  SRR4302543.2268767/1‑94 (MQ=60)

         TGGCTGGCACCGAATCTGGGGTATGACGCGGACTGATTCACAAATCTGTCACTTTTCCTTACAACAAACAGGGCGCTCAATGAGCGCCCTGTCT                                                                                      <  SRR4302543.3930210/94‑1 (MQ=60)

         TGGCTGGCACCGAATCTGGGGTATGACGCGGACTGATTCACAAATCTGTCACTTTTCCTTACAACAAACAGGGCGCTCAATGAGCGCCCTGTCT                                                                                      <  SRR4302543.1136522/94‑1 (MQ=60)

           GCTGGCACCGAATCTGGGGTATGACGCGGACTGATTCACAAATCTGTCACTTTTCCTTACAACAAACAGGGCGCTCAATGAGAGCCCTGTCTCT                                                                                    >  SRR4302543.214068/1‑94 (MQ=60)

            CTGGCACCGAATCTGGGGTATGACGCGGACTGATTCACAAATCTGTCACTTTTCCTTACAACAAACAGGGCGCTCAATGAGCGCCCTGTCTCTT                                                                                   >  SRR4302543.5016396/1‑94 (MQ=60)

             tggcccatAATCTGGTGTATGCCGCGGACTGATTCACAAATCTGTCACTTTTCCTTAAAACAAACAGGGCGCTCAATGAGCGCCCTGTCTCTTT                                                                                  <  SRR4302543.3099225/86‑1 (MQ=60)

               GCACCGAATCTGGGGTATGACGCGGACTGATTCACAAATCTGTCACTTTTCCTTACAACAAACAGGGCGCTCAATGAGCGCCCTGTCTCTTTAT                                                                                <  SRR4302543.2604961/94‑1 (MQ=60)

               GCACCGAATCTGGGGTATGACGCGGACTGATTCACAAATCTGTCACTTTTCCTTACAACAAACAGGGCGCTCAATGAGCGCCCTGTCTCTTTAT                                                                                <  SRR4302543.2526217/94‑1 (MQ=60)

               GCACCGAATCTGGGGTATGACGCGGACTGATTCACAAATCTGTCACTTTTCCTTACAACAAACAGGGCGCTCAATGAGCGCCCTGTCTCTTTAT                                                                                <  SRR4302543.5223949/94‑1 (MQ=60)

                CACCGAATCTGGGTTATGACGCGGACTGATTCACAAATCTGTCACTTTTCCTTACAACAAACAGGGCGCTCAATGAGCGCCCTGTCTCTTTATT                                                                               <  SRR4302543.1298666/94‑1 (MQ=60)

                CACCGAATCTGGGGTATGACGCGGACTGATTCACAAATCTGTCACTTTTCCTTACAACAAACAGGGCGCTCAATGAGCGCCCTGTCTCTTTATT                                                                               <  SRR4302543.2100554/94‑1 (MQ=60)

                CACCGAATCTGGGGTATGACGCGGACTGATTCACAAATCTGTCACTTTTCCTTACAACAAACAGGGCGCTCAATGAGCGCCCTGTCTCTTTATT                                                                               >  SRR4302543.2682626/1‑94 (MQ=60)

                CACCGAATCTGGGGTATGACGCGGACTGATTCACAAATCTGTCACTTTTCCTTACAACAAACAGGGCGCTCAATGAGCGCCCTGTCTCTTTATT                                                                               >  SRR4302543.6788423/1‑94 (MQ=60)

                CACCGAATCTGGGGTATGACGCGGACTGATTCACAAATCTGTCACTTTTCCTTACAACAAACAGCGCGCTCAATGAGCGCCCTGTCTCTTTATT                                                                               >  SRR4302543.6788424/1‑94 (MQ=60)

                 ACCGAATCTGGGGTATGACGCGGACTGATTCACAAATCTGTCACTTTTCCTTACAACAAACAGGGCGCTCAATGAGCGCCCTGTCTCTTTATTA                                                                              <  SRR4302543.922578/94‑1 (MQ=60)

                  CCGAATCTGGGGTATGACGCGGACTGATTCACAAATCTGTCACTTTTCCTTACAACAAACAGGGCGCTCAATGAGCGCCCTGTCTCTTTATTAA                                                                             <  SRR4302543.6772096/94‑1 (MQ=60)

                  CCGAATCTGGGGTATGACGCGGACTGATTCACAAATCTGTCACTTTTCCTTACAACAAACAGAGCGCTCAATGAGAGCCCTGTCTCTTTATTAA                                                                             >  SRR4302543.117463/1‑94 (MQ=60)

                   CGAATCTGGGGTATGACGCGGACTGATTCACAAATCTGTCACTTTTCCTTACAACAAACAGGGCGCTCAATGAGTGCCCTGTCTCTTTATTAAT                                                                            >  SRR4302543.7721760/1‑94 (MQ=60)

                    GAATCTGGGGTATGACGCGGACTGATTCACAAATCTGTCCCTTTTCCTTACAACAAACAGGGCGCTCAATGAGCCCCCTGTCTCTTTATTAATA                                                                           <  SRR4302543.2489705/94‑1 (MQ=60)

                     agtctgcgtTATGACGCGGACTGATCCACAATTCTGTCACTTTTCCTAACAACAAACAGGCCGCTCAATGAGCCCCCTGTCTCTTTATTAATAT                                                                          <  SRR4302543.3615599/85‑1 (MQ=60)

                     AATCTGGGGTATGACGCGGACTGATTCACAAATCTGTCACTTTTCCTTACAACAAACAGGGCGCTCCATGAGGGCCCTGTCTCTTTTTTTATAT                                                                          >  SRR4302543.5646365/1‑94 (MQ=60)

                     AATCTGGGGTATGACGCGGACTGATTCACAAATCTGTCACTTTTCCTTACAACAAACAGGGCGCTCAATGAGCGCCCTGTCTCTTTATTAATAT                                                                          >  SRR4302543.7695455/1‑94 (MQ=60)

                     AATCGGGGGTATGACGCGGACTGATTCACAAATCTGTCACTTTTCCTTACAACAAACAGGGCGCTCAATGAGCGCCCTGTCTCTTTATTAATAT                                                                          <  SRR4302543.1112951/94‑1 (MQ=60)

                      ATCTGGGGTATGACGCGGACTGATTCACAAATCTGTCACTTTTCCTTACAACAAACAGGGCGCTCCATGAGCGCCCCGTCTCTTTttttatatt                                                                         >  SRR4302543.2914921/1‑85 (MQ=60)

                      ATCTGGGGTATGACGCGGACTGATTCACAAATCTGTCACTTTTCCTTACAACAAACAGGGCGCTCAATGAGCGCCCTGTCTCTTTATTAATATG                                                                         >  SRR4302543.2066809/1‑94 (MQ=60)

                       TCTGGGGTATGACGCGGCCGGATTCACAATTCTGTCACTTTTCCTTACAACAACCAGGGCCCTCAATGAGCGCCCTGTCTCTTTATTAATATGA                                                                        <  SRR4302543.6547133/94‑1 (MQ=60)

                       TCTGGGGTATGACGCGGACTGATTCACAAATCTGTCACTTTTCCTTACAACAAACAGGGCGCTCAATGAGCGCCCTGTCTCTTTATTAATATGA                                                                        >  SRR4302543.4702127/1‑94 (MQ=60)

                       TCTGGGGTATGACGCGGACTGATTCACAAATCTGTCACTTTTCCTTACAACAAACAGGGCGCTCAATGAGCGCCCTGTCTCTTTATTAATATGA                                                                        >  SRR4302543.4702126/1‑94 (MQ=60)

                           GGGTATGACGCGGACTGATTCACAAATCTGTCACTTTTCCTTACAACAAACAGGGCGCTCAATGAGCGCCCTGTCTCTTTATTAATATGAAACA                                                                    <  SRR4302543.6189753/94‑1 (MQ=60)

                           GGGTATGACGCGGACTGATTCACAAATCTGTCACTTTTCCTTACAACAAACAGGGCGCTCAATGAGCGCCCTGTCTCTTTATTAATATGAAACA                                                                    >  SRR4302543.2797639/1‑94 (MQ=60)

                           GGGTATGACGCGGACTGATTCACAAATCTGTCACTTTTCCTTACAACAAACAGGGCGCTCAATGAGCGCCCTGTCTCTTTATTAATATGAAACA                                                                    <  SRR4302543.4158885/94‑1 (MQ=60)

                                TGACGCGGACTGATTCACAAATCTGTCACTTTTCCTTACAACAAACAGGGCGCTCAATGAGCGCCCTGTCTCTTTATTAATATGAAACACTTAT                                                               <  SRR4302543.6744647/94‑1 (MQ=60)

                                TGACGCGGACTGATTCACAAATCTGTCACTTTTCCTTACAACAAACAGGGCGCTCAATGAGCGCCCTGTCTCTTTATTAATATGAAACACTTAT                                                               <  SRR4302543.6744648/94‑1 (MQ=60)

                                TGACGCGGACTGATTCACAAATCTGTCACTTTTCCTTACAACAAACAGGGCGCTCAATGAGCGCCCTGTCTCTTTATTAATATGAAACACTTAT                                                               >  SRR4302543.4917382/1‑94 (MQ=60)

                                  CCGCGGATTGATCCACAATTCTGTCATTTTCCCTAACAACAACCAGGGCGCTCAATGGGCGCCCTGTCTCTTTATTAATATGAAACACTTATAC                                                             <  SRR4302543.1518388/94‑1 (MQ=50)

                                  ACGCGGACTGATTCACAAATCTGTCACTTTTCCTTACAACAAACAGGGCGGTCAATGAGCGCCCTGTCTCTTTTTTTATATGAAACACTTATAC                                                             >  SRR4302543.318839/1‑94 (MQ=60)

                                  ACGCGGACTGATTCACAAATCTGTCACTTTTCCTTACAACAAACAGGGCGCTCAATGAGCGCCCTGTCTCTTTATTAATATGAAACACTTATAC                                                             <  SRR4302543.2678634/94‑1 (MQ=60)

                                  ACGCGGACTGATTCACAAATCTGTCACTTTTCCTTACAACAAACAGGGCGCTCAATGAGCGCCCTGTCTCTTTATTAATATGAAACACTTATAC                                                             <  SRR4302543.2654311/94‑1 (MQ=60)

                                  ACGCGGACTGATTCACAAATCTGTCACTTTTCCTTACAACAAACAGGGCGCTCAATGAGCGCCCTGTCTCTTTATTAATATGAAACACTTATAC                                                             <  SRR4302543.7866539/94‑1 (MQ=60)

                                  ACGCGGACTGATTCACAAATCTGTCACTTTTCCTTACAACAAACAGGGCGATCAATAAGCGCCCTGTCTCTTTATTAATATGAAACACTTATAC                                                             <  SRR4302543.4989534/94‑1 (MQ=60)

                                      GGACTGATTCACAAATCTGTCACTTTTCCTTACAACAAACAGGGCGCTCAATGAGCGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGA                                                         >  SRR4302543.4637948/1‑94 (MQ=60)

                                      GGACTGATTCACAAATCTGTCACTTTTCCTTACAACAAACAGGGCGCTCAATGAGCGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGA                                                         >  SRR4302543.547573/1‑94 (MQ=60)

                                      GGACTGATTCACAAATCTGTCACTTTTCCTTACAACAAACAGGGCGCTCAATGAGCGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGA                                                         >  SRR4302543.3732638/1‑94 (MQ=60)

                                      GGACTGATTCACAAATCTGTCACTTTTCCTTACAACAAACAGGGCGCTCAATGAGCGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGA                                                         >  SRR4302543.2185744/1‑94 (MQ=60)

                                      GGACTGATTCACAAATCTGTCACTTTTCCTTACAACAAACAGGGCGCTCAATGAGCGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGA                                                         >  SRR4302543.6246940/1‑94 (MQ=60)

                                      GGACTGATTCACAAATCTGTCACTTTTCCTTACAACAAACAGGGCGCTCAATGAGCGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGA                                                         >  SRR4302543.6591452/1‑94 (MQ=60)

                                      GGACTGATTCACAAATCTGTCACTTTTCCTTACAACAAACAGGGCGCTCAATGAGCGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGA                                                         >  SRR4302543.7570735/1‑94 (MQ=60)

                                       GACTGATTCACAAATCTGTCACTTTTCCTTACAACAAACAGGGCGCTCAATGAGCGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAA                                                        <  SRR4302543.5273105/94‑1 (MQ=60)

                                         CTGATTCACAAATCTGTCACTTTTCCTTACAACAAACAGGGCGCTCAATGAGCGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAAC                                                      >  SRR4302543.912258/1‑94 (MQ=60)

                                           TATTCACAAATCTGTCACTTTTCCTTACAACAAACAGGGCGCTCAATGAGCGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAACAG                                                    <  SRR4302543.2077418/94‑1 (MQ=60)

                                           GATTCACAAATCTGTCACTTTTCCTTACAACAAACAGGGCGCTCAATGAGCGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAACAG                                                    <  SRR4302543.7705301/94‑1 (MQ=60)

                                           GATTCACAAATCTGTCACTTTTCCTTACAACAAACAGGGCGCTCAATGAGCGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAACAG                                                    >  SRR4302543.4952773/1‑94 (MQ=60)

                                           GATTCACAAATCTGTCACTTTTCCTAACAACAAACGGGGCGCTCAATGGGCGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAACAG                                                    <  SRR4302543.2160000/94‑1 (MQ=60)

                                            ATTCACAAATCTGTCACTTTTCCTTACAACAAACAGGGCGCTCAATGAGCGCCCTGTCTCTTTATTTATATGAAACACTTATACTGGAAACAGG                                                   >  SRR4302543.7213015/1‑94 (MQ=60)

                                            ATTCACAAATCTGTCACTTTTCCTTACAACAAACAGGGCGCTCAATGAGCGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAACAGG                                                   <  SRR4302543.4871262/94‑1 (MQ=60)

                                             TTCACAAATCTGTCACTTTTCCTTACAACAAACAGGGCGCTCAATGAGCGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAACAGGC                                                  <  SRR4302543.3700999/94‑1 (MQ=60)

                                              TCACAAATCTGTCACTTTTCCTTACAACAAACAGGGCGCTCAATGAGCGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAACAGGCT                                                 <  SRR4302543.7471418/94‑1 (MQ=60)

                                               CACAAATCTGTCACTTTTCCTTACAACAAACAGGGCGCTCAATGAGCGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAACAGGCTG                                                >  SRR4302543.4069850/1‑94 (MQ=60)

                                                 CAAATCTGTCACTTTTCCTTACAACAAACAGGGCGCTCAATGAGGGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAACAGGCTGGA                                              >  SRR4302543.4624207/1‑94 (MQ=60)

                                                 CAAATCTGTCACTTTTCCTTACAACAAACAGGGCGCTCAATGAGGGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAACAGGCTGGA                                              >  SRR4302543.7231390/1‑94 (MQ=60)

                                                 CAAATCTGTCACTTTTCCTTACAACAAACAGGGCGCTCAATGAGCGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAACAGGCTGGA                                              <  SRR4302543.2447612/94‑1 (MQ=60)

                                                   AATCTGTCACTTTTCCTTACAACAAACAGGGCGCTCAATGAGCGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAACAGGCTGGGAT                                            >  SRR4302543.496211/1‑94 (MQ=60)

                                                   AATCTGTCACTTTTCCTTACAACAAACAGGGCGCTCAATGAGCGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAACAGGCTGGAAT                                            >  SRR4302543.4090549/1‑94 (MQ=60)

                                                    atctctcacttctgctaaaaccaagCAGGGCCCTCAATGAGCCCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAACAGGCTGGAATA                                           <  SRR4302543.3510386/69‑1 (MQ=60)

                                                    ATCTGTCACTTTTCCTTACAACAAACAGGGCGCTCAATGAGCGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAACAGGCTGGAATA                                           <  SRR4302543.3510393/94‑1 (MQ=60)

                                                     tctttcttTTTTCCTTACAACAAACGGGCCGCTAAATGAGCGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAACAGGCTGGAATAA                                          <  SRR4302543.4459699/86‑1 (MQ=60)

                                                     TCTGTCACTTTTCCTTACAACAAACAGGGCGCTCAATGAGCGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAACAGGCTGGAATAA                                          >  SRR4302543.6886460/1‑94 (MQ=60)

                                                       TGTCACTTTTCCTTACAACAAACAGGGCGCTCAATGAGCGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAACAGGCTGGAATAATC                                        >  SRR4302543.1111931/1‑94 (MQ=60)

                                                       TGTCACTTTTCCTTACAACAAACAGGGCGCTAAATGAGCGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAACAGGCTGGAATAATC                                        <  SRR4302543.6139718/94‑1 (MQ=60)

                                                         TCACTTTTCCTTACAACAAACAGGGCGCTCAATGAGCGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAACAGGCTGGAATAATCTT                                      <  SRR4302543.7804942/94‑1 (MQ=60)

                                                          ccttttcccagaaaagaaaggggcCGCTCAATGAGCGCGCTGTCTCTTTATTAATATGAAACCCTTATACTGGAAACAGGCTGGAATAATCTTA                                     <  SRR4302543.5966170/70‑1 (MQ=60)

                                                          CACTTTTCCTTACAACAAACAGGGCGCTCAATGAGCGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAACAGGCTGGAATAATCTTA                                     >  SRR4302543.3274656/1‑94 (MQ=60)

                                                          CACTTTTCCTTACAACAAACAGGGCGCTCAATGAGCGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAACAGGCTGGAATAATCTTA                                     <  SRR4302543.5045028/94‑1 (MQ=60)

                                                           ACTTTTCCTTACAACAAACAGGGCGCTCAATGAGCGCCCCGTCTCTTTATTTATATGAAACACTTATACTGGAAACAGGCTGGGATAATCTTAG                                    >  SRR4302543.35644/1‑94 (MQ=60)

                                                            CTTTTCCTTACAACAAACAGGGCGCTCAATGAGCGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAACAGGCTGGAATAATCTTAGC                                   <  SRR4302543.3459037/94‑1 (MQ=60)

                                                            CTTTTCCTTACAACAAACAGGGCGCTCAATGAGCGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAACAGGCTGGAATAATCTTAGC                                   >  SRR4302543.3525432/1‑94 (MQ=60)

                                                             TTTTCCTTACAACAAACAGGGCGCTCAATGAGCGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAACAGGCTGGAATAATCTTAGCC                                  <  SRR4302543.2776260/94‑1 (MQ=60)

                                                               TTCCTTACAACAAACAGGGCGCTCAATGAGCGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAACAGGCTGGAATAATCTTAGCCGG                                <  SRR4302543.4130665/94‑1 (MQ=60)

                                                                TCCTTACAACAAACAGGGCGCTCAATGAGCGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAACAGGCTGGAATAATCTTAGCCGGA                               >  SRR4302543.4804178/1‑94 (MQ=60)

                                                                TCCTTACAACAAACAGGGCGCTCAATGAGCGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAACAGGCTGGAATAATCTTAGCCGGA                               >  SRR4302543.2088405/1‑94 (MQ=60)

                                                                  CTTACAACAAACAGGGCGCTCAATGAGCGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAACAGGCTGGGATAATCTTAGCCGGAAA                             >  SRR4302543.7266403/1‑94 (MQ=60)

                                                                  CTTACAACAAACAGGGCGCTCAATGAGCGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAACAGGCTGGAATAATCTTAGCCGGAAA                             >  SRR4302543.665613/1‑94 (MQ=60)

                                                                    taaaacaacaggggctCTCAATGAGCCCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAACAGGCTGGAATAATCTTAGCCGGAAAAA                           <  SRR4302543.6220419/78‑1 (MQ=60)

                                                                    TACAACAAACAGGGCGCTCAATGAGCGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAACAGGCTGGAATAATCTTAGCCGGAAAAA                           >  SRR4302543.7986732/1‑94 (MQ=60)

                                                                    TACAACAAACAGGGCGCTCAATGAGCGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAACAGGCTGGAATAATCTTAGCCGGAAAAA                           >  SRR4302543.2510871/1‑94 (MQ=60)

                                                                    TAAAACAAACAGGGCGCTAAAGGAGCCCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAACAGGCTGGAATAATCTTAGCCGGAAAAA                           <  SRR4302543.1491227/94‑1 (MQ=60)

                                                                     ACAACAAACAGGGCGCTCAATGAGCGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAACAGGCTGGAATAATCTTAGCCGGAAAAAC                          >  SRR4302543.6798101/1‑94 (MQ=60)

                                                                     ACAACAAACAGGGCGCTCAATGAGCGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAACAGGCTGGAATAATCTTAGCCGGAAAAAC                          <  SRR4302543.1047798/94‑1 (MQ=60)

                                                                     ACAACAAACAGGGCGCTCAATGAGCGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAACAGGCTGGAATAATCTTAGCCGGAAAAAC                          <  SRR4302543.6911974/94‑1 (MQ=60)

                                                                     ACAACAAACAGGGCGCTCAATGAGCGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAACAGGCTGGAATAATCTTAGCCGGAAAAAC                          <  SRR4302543.1158674/94‑1 (MQ=60)

                                                                     ACAACAAACAGGGCGCTAAATGAGCGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAACAGGCTGGAATAATCTTAGCCGGAAAAAC                          <  SRR4302543.166518/94‑1 (MQ=60)

                                                                      CAACAAACAGGGCGCTCAATGAGCGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAACAGGCTGGAATAATCTTAGCCGGAAAAACG                         >  SRR4302543.1015373/1‑94 (MQ=60)

                                                                      CAACAAACAGGGCGCTCAATGAGCGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAACAGGCTGGAATAATCTTAGCCGGAAAAACG                         >  SRR4302543.1292560/1‑94 (MQ=60)

                                                                      CAACAAACAGGGCGCTCAATCAGCGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAACAGGCTGGAATAATCTTAGCCGGAAAAACG                         >  SRR4302543.4713861/1‑94 (MQ=60)

                                                                        ACAAACAGGGCGCTCAATGAGCGCCCCGTCTCTTTATTTATATTAAACACTTATACTTGAAACAAGCTGGGATAATCTTAGCCGGAAAAACGAA                       >  SRR4302543.6118647/1‑94 (MQ=60)

                                                                        ACAAACAGGGCGCTCAATGAGCCCCCTGTCTCTTTATTTATATGGAACACTTATATTGGAAACAGGCTGGGATAAACTTAGCCGGAAAAAacaa                       >  SRR4302543.764214/1‑90 (MQ=60)

                                                                         CAAACAGGGCGCTCAATGAGCGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAACAGGCTGGAATAATCTTAGCCGGAAAAACGAAT                      <  SRR4302543.4863774/94‑1 (MQ=60)

                                                                         CAAACAGGGCGCTCAATGAGCGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAACAGGCTGGAATAATCTTAGCCGGAAAAACGAAT                      <  SRR4302543.3774908/94‑1 (MQ=60)

                                                                         CAAACAGGGCGCTCAATGAGCGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAACAGGCTGGAATAATCTTAGCCGGAAAAAAGAAT                      >  SRR4302543.4263747/1‑94 (MQ=60)

                                                                         CAAACAGGGCGCTCAATGACCGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAACAGGCTGGAATAATCTTAGCCGGAAAAACGAAT                      <  SRR4302543.935242/94‑1 (MQ=60)

                                                                         AAAACAGGGCGCTCAATGAGCGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAACAGGCTGGAATAATCTTAGCCGGAAAAACGAAT                      <  SRR4302543.4863773/94‑1 (MQ=60)

                                                                          AAACAGGGCGCTCAATGAGCGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAACAGGCTGGAATAATCTTAGCCGGAAAAACGAATG                     <  SRR4302543.6646280/94‑1 (MQ=60)

                                                                          AAACAGGGCGCTCAATGAGCGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAACAGGCTGGAATAATCTTAGCCGGAAAAACGAATG                     <  SRR4302543.6375475/94‑1 (MQ=60)

                                                                          AAACAGGGCGCTCAATGAGCGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAACAGGCTGGAATAATCTTAGCCGGAAAAACGAATG                     >  SRR4302543.1425769/1‑94 (MQ=60)

                                                                          AAACAGGGCGCTCAATGAGCGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAACAGGCTGGAATAATCTTAGCCGGAAAAACGAATG                     >  SRR4302543.5570626/1‑94 (MQ=60)

                                                                           AACAGGGCGCTCAATGGGCGCCCTGTCTCTTTATTAATATGAAACCATTATACTGGAAACAGGCTGGGATAATGTTAGACGGAAAAACGAATAT                    >  SRR4302543.7162579/1‑94 (MQ=60)

                                                                           AACAGGGCGCTCAATGAGCGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAACAGGCTGGAATAATCTTAGCCGGAAAAACGAATGT                    <  SRR4302543.1277982/94‑1 (MQ=60)

                                                                              AGGGCGCTCAATGAGCGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAACAGGCTGGAATAATCTTAGCCGGAAAAACGAATGTCAA                 >  SRR4302543.2755108/1‑94 (MQ=60)

                                                                              AGGGCGCTCAATGAGCGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAACAGGCTGGAATAATCTTAGCCGGAAAAACGAATGTCAA                 >  SRR4302543.1295096/1‑94 (MQ=60)

                                                                              AGGGCGCTCAATGAGCGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAACAGGCTGGAATAATCTTAGCCGGAAAAACGAATGTCAA                 >  SRR4302543.6193810/1‑94 (MQ=60)

                                                                                GGCGCTCAATGAGCGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAACAGGCTGGAATAATCTTAGCCGGAAAAACGAATGTCAAAA               <  SRR4302543.7774933/94‑1 (MQ=60)

                                                                                 GCGCTCAATGAGCGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAACAGGCTGGAATAATCTTAGCCGGAAAAACGAATGTCAAAAG              <  SRR4302543.591676/94‑1 (MQ=60)

                                                                                    CTCAATGAGCGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAACAGGCTGGAATAATCTTAGCCGGAAAAACGAATGTCAAAAGGCA           >  SRR4302543.3343880/1‑94 (MQ=60)

                                                                                        ATGAGCGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAACAGGCTGGAATAATCTTAGCCGGAAAAACGAATGTCAAAAGGCACAGT       >  SRR4302543.6370616/1‑94 (MQ=60)

                                                                                           AGCGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAACAGGCTGGAATAATCTTAGCCGGAAAAACGAATGTCAAAAGGCACAGTAAA    <  SRR4302543.4036472/94‑1 (MQ=60)

                                                                                            GCGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAACAGGCTGGAATAATCTTAGCCGGAAAAACGAATGTCAAAAGGCACAGTAAAG   <  SRR4302543.7589765/94‑1 (MQ=60)

                                                                                             CGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAACAGGCTGGAATAATCTTAGCCGGAAAAACGAATGTCAAAAGGCACAGTAAAGA  >  SRR4302543.1454002/1‑94 (MQ=60)

                                                                                             CGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAACAGGCTGGAATAATCTTAGCCGGAAAAACGAATGTCAAAAGGCACAGTAAAGA  >  SRR4302543.7143613/1‑94 (MQ=60)

GTTGAGCGCTGGCTGGCACCGAATCTGGGGTATGACGCGGACTGATTCACAAATCTGTCACTTTTCCTTACAACAAACAGGGCGCTCAATGAGTGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAACAGGCTGGAATAATCTTAGCCGGAAAAACGAATGTCAAAAGGCACAGTAAAGA  >  NC_000913/4225046‑4225232

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 2 ≤` `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 31 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`