Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 1696 279.3 15874187 97.8% 15524954 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,363,427 T→C T309A (ACT→GCT)  dcuA ← anaerobic dicarboxylate transport

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,363,4270TC99.5% 1238.6 / NA 390T309A (ACT→GCT) dcuAanaerobic dicarboxylate transport
Reads supporting (aligned to +/- strand):  ref base T (0/0);  major base C (176/212);  minor base A (2/0);  total (178/212)
Fisher's exact test for biased strand distribution p-value = 2.08e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 397 total aligned reads displayed.

AGAAGAAGATGACGGCCAGCAGCCACGGATGACCCTGAATCACTTCACCAGCGGTATCTTTGATCCAGTCGATGTTGTTGGAAACGAAAGTATCGCCCAGCCACGCAACACCCAGGATACAAATACAGGCGCTCATACCTGCTTTGAAGGTGCTGGAGTTGAGGATGTTGTCGGTATCGA  >  NC_000913/4363337‑4363516
                                                                                          |                                                                                         
agaagaagaTGACGGCCAGCAGCCACGGATGACCCTGAATCACTTCACCAGCGGTATCTTTGATCCAGTCGATGTTGTTGGAAACGAAAGCAt                                                                                         <  2:4243458/93‑1 (MQ=255)
 gaagaagaTGACGGCCAGCAGCCACGGATGACCCTGAATCACTTCACCAGCGGTATCTTTGATCCAGTCGATGTTGTTGGAAACGAAAGCATc                                                                                        <  1:893004/93‑1 (MQ=255)
 gaagaagaTGACGGCCAGCAGCCACGGATGACCCTGAATCACTTCACCAGCGGTATCTTTGATCCAGTCGATGTTGTTGGAAACGAAAGCATc                                                                                        >  1:3558658/1‑93 (MQ=255)
 aaagaagaTGACGGCCAGCAGCCACGGATGACCCTGAATCACTTCACCAGCGGTATCTTTGATCCAGTCGATGTTGTTGGAAACGAAAGCATc                                                                                        <  2:5596984/92‑1 (MQ=255)
    gaagaTGACGGCCAGCAGCCACGGATGACCCTGAATCACTTCACCAGCGGTATCTTTGATCCAGTCGATGTTGTTGGAAACGAAAGCATCGcc                                                                                     <  2:7385578/93‑1 (MQ=255)
     aagaTGACGGCCAGCAGCCACGGATGACCCTGAATCACTTCACCAGCGGTATCTTTGATCCAGTCGATGTTGTTGGAAACGAAAGCATCGccc                                                                                    <  2:1632569/93‑1 (MQ=255)
     aagaTGACGGCCAGCAGCCACGGATGACCCTGAATCACTTCACCAGCGGTATCTTTGATCCAGTCGATGTTGTTGGAAACGAAAGCATCGccc                                                                                    >  2:3481905/1‑93 (MQ=255)
      agaTGACGGCCAGCAGCCACGGATGACCCTGAATCACTTCACCAGCGGTATCTTTGATCCAGTCGATGTTGTTGGAAACGAAAGCATCGCCCa                                                                                   >  2:3679025/1‑93 (MQ=255)
        aTGACGGCCAGCAGCCACGGATGACCCTGAATCACTTCACCAGCGGTATCTTTGATCCAGTCGATGTTGTTGGAAACGAAAGCATCGCCCAGc                                                                                 >  2:675477/1‑93 (MQ=255)
         tGACGGCCAGCAGCCACGGATGACCCTGAATCACTTCACCAGCGGTATCTTTGATCCAGTCGATGTTGTTGGAAACGAAAGCATCGGCCAGcc                                                                                >  2:230945/1‑93 (MQ=255)
           aCGGCCAGCAGCCACGGATGACCCTGAATCACTTCACCAGCGGTATCTTTGATCCAGTCGATGTTGTTGGAAACGAAAGCATCGCCCAGCCAc                                                                              <  1:3994483/93‑1 (MQ=255)
               ccAGCAGCCACGGATGACCCTGAATCACTTCACCAGCGGTATCTTTGATCCAGTCGATGTTGTTGGAAACGAAAGCATCGCCCAGCCACGCaa                                                                          >  2:3317730/1‑93 (MQ=255)
                cagcagCCACGGATGACCCTGAATCACTTCACCAGCGGTATCTTTGATCCAGTCGATGTTGTTGGAAACGAAAGCATCGCCCAGCCACGCAac                                                                         <  2:5788879/93‑1 (MQ=255)
                cagcagCCACGGATGACCCTGAATCACTTCACCAGCGGTATCTTTGATCCAGTCGATGTTGTTGGAAACGAAAGCATCGCCCAGCCACGCAac                                                                         <  1:286757/93‑1 (MQ=255)
                aagcagcCACGGATGACCCTGAATCACTTCACCAGCGGTATCTTTGATCCAGTCGATGTTGTTGGAAACGAAAGCATCCCCCAGCCATGCAac                                                                         >  1:1257556/2‑93 (MQ=255)
                 agcagcCACGGATGACCCTGAATCACTTCACCAGCGGTATCTTTGATCCAGTCGATGTTGTTGGAAACGAAAGCATCGCCCAGCCACGCAaca                                                                        <  1:1638917/93‑1 (MQ=255)
                  gcagcCACGGATGACCCTGAATCACTTCACCAGCGGTATCTTTGATCCAGTCGATGTTGTTGGAAACGAAAGCATCGCCCAGCCACGCAacac                                                                       >  1:2219468/1‑93 (MQ=255)
                  gcagcCACGGATGACCCTGAATCACTTCACCAGCGGTATCTTTGATCCAGTCGATGTTGTTGGAAACGAAAGCATCGCCCAGCCACGCAacac                                                                       >  2:3085381/1‑93 (MQ=255)
                   cagcCACGGATGACCCTGAATCACTTCACCAGCGGTATCTTTGATCCAGTCGATGTTGTTGGAAACGAAAGCATCGCCCAGCCACGCAACAcc                                                                      <  1:5807955/93‑1 (MQ=255)
                   cagcCACGGATGACCCTGAATCACTTCACCAGCGGTATCTTTGATCCAGTCGATGTTGTTGGAAACGAAAGCATCGCCCAGCCACGCAACAcc                                                                      <  1:321289/93‑1 (MQ=255)
                   cagcCACGGATGACCCTGAATCACTTCACCAGCGGTATCTTTGATCCAGTCGATGTTGTTGGAAACGAAAGCATCGCCCAGCCACGCAACAcc                                                                      <  1:7611680/93‑1 (MQ=255)
                      ccACGGATGACCCTGAATCTCTTCACCAGCGGTATCTTTGATCCAGTCGATGTTGTTGGAAACGAAAGCATCGCCCAGCCACGCAACACCCAg                                                                   <  1:7122764/93‑1 (MQ=255)
                      ccACGGATGACCCTGAATCACTTCACCAGCGGTATCTTTGATCCAGTCGATGTTGTTGGAAACGAAAGCATCGCCCAGCCACGCAACACCCAg                                                                   <  2:1289708/93‑1 (MQ=255)
                      ccACGGATGACCCTGAATCACTTCACCAGCGGTATCTTTGATCCAGTCGATGTTGTTGGAAACGAAAGCATCGCCCAGCCACGCAACACCCAg                                                                   >  2:6412002/1‑93 (MQ=255)
                       cACGGATGACCCTGAATCACTTCACCAGCGGTATCTTTGATCCAGTCGATGTTGTTGGAAACGAAAGCATCGCCCAGCCACGCAACACCCAgg                                                                  <  2:1778012/93‑1 (MQ=255)
                        aCGGATGACCCTGAATCACTTCACCAGCGGTATCTTTGATCCAGTCGATGTTGTTGGAAACGAAAGCATCGCCCAGCCACGCAACACCCAGGa                                                                 >  1:5160270/1‑93 (MQ=255)
                        aCGGATGACCCTGAATCACTTCACCAGCGGTATCTTTGATCCAGTCGATGTTGTTGGAAACGAAAGCATCGCCCAGCCACGCAACACCCAGGa                                                                 >  2:6266329/1‑93 (MQ=255)
                           gATGACCCTGAATCACTTCACCAGCGGTATCTTTGATCCAGTCGATGTTGTTGGAAACGAAAGCATCGCCCAGCCACGCAACACCCAGGATAc                                                              <  1:1786252/93‑1 (MQ=255)
                             tGACCCTGAATCACTTCACCAGCGGTATCTTTGATCCAGTCGATGTTGTTGGAAACGAAAGCATCGCCCAGCCACGCAACACCCAGGATACaa                                                            >  1:4849422/1‑93 (MQ=255)
                               aCCCTGAATCACTTCACCAGCGGTATCTTTGATCCAGTCGATGTTGTTGGAAACGAAAGCATCGCCCAGCCACGCAACACCCAGGATACAAAt                                                          >  2:5421256/1‑93 (MQ=255)
                                cccTGAATCACTTCACCAGCGGTATCTTTGATCCAGTCGATGTTGTTGGAAACGAAAGCATCGCCCAGCCACGCAACACCCAGGATACAAATa                                                         <  1:2807371/93‑1 (MQ=255)
                                 ccTGAATCACTTCACCAGCGGTATCTTTGATCCAGTCGATGTTGTTGGAAACGAAAGCATCGCCCAGCCACGCAACACCCAGGATACAAATAc                                                        <  2:7113212/93‑1 (MQ=255)
                                 ccTGAATCACTTCACCAGCGGTATCTTTGATCCAGTCGATGTTGTTGGAAACGAAAGCATCGCCCAGCCACGCAACACCCAGGATACAAATAc                                                        <  2:7876904/93‑1 (MQ=255)
                                 ccTGAATCACTTCACCAGCGGTATCTTTGATCCAGTCGATGTTGTTGGAAACGAAAGCATCGCCCAGCCACGCAACACCCAGGATACAAATAc                                                        >  2:264229/1‑93 (MQ=255)
                                  cTGAATCACTTCACCAGCGGTATCTTTGATCCAGTCGATGTTGTTGGAAACGAAAGCATCGCCCAGCCACGCAACACCCAGGATACAAATACa                                                       >  1:7355262/1‑93 (MQ=255)
                                   tGAATCACTTCACCAGCGGTATCTTTGATCCAGTCGATGTTGTTGGAAACGAAAGCATCGCCCAGCCACGCAACACCCAGGATACAAATACAg                                                      <  1:5241018/93‑1 (MQ=255)
                                   tGAATCACTTCACCAGCGGTATCTTTGATCCAGTCGATGTTGTTGGAAACGAAAGCATCGCCCAGCCACGCAACACCCAGGATACAAATACAg                                                      >  1:3707830/1‑93 (MQ=255)
                                   tGAATCACTTCACCAGCGGTATCTTTGATCCAGTCGATGTTGTTGGAAACGAAAGCATCGCCCAGCCACGCAACACCCAGGATACAAATACAg                                                      <  1:1931557/93‑1 (MQ=255)
                                   tGAATCACTTCACCAGCGGTATCTTTGATCCAGTCGATGTTGTTGGAAACGAAAGCATCGCCCAGCCACGCAACACCCAGGATACAAATACAg                                                      <  1:4122956/93‑1 (MQ=255)
                                    gAATCACTTCACCAGCGGTATCTTTGATCCAGTCGATGTTGTTGGAAACGAAAGCATCGCCCAGCCACGCAACACCCAGGATACAAATACAgg                                                     <  1:1879385/93‑1 (MQ=255)
                                    gAATCACTTCACCAGCGGTATCTTTGATCCAGTCGATGTTGTTGGAAACGAAAGCATCGCCCAGCCACGCAACACCCAGGATACAAATACAgg                                                     >  1:5610511/1‑93 (MQ=255)
                                    gAATCACTTCACCAGCGGTATCTTTGATCCAGTCGATGTTGTTGGAAACGAAAGCATCGCCCAGCCACGCAACACCCAGGATACAAATACAgg                                                     >  1:1371651/1‑93 (MQ=255)
                                    gAATCACTTCACCAGCGGTATCTTTGATCCAGTCGATGTTGTTGGAAACGAAAGCATCGCCCAGCCACGCAACACCCAGGATACAAATACAgg                                                     >  1:4360027/1‑93 (MQ=255)
                                     aaTCCCTTCACCAGCGGTATCTTTGATCCAGTCGATGTTGTTGGAAACGAAAGCATCGCCCAGCCACGCAACACCCAGGATACAAATACAGgc                                                    <  2:628015/93‑1 (MQ=255)
                                     aaTCACTTCACCAGCGGTATCTTTGATCCAGTCGATGTTGTTGGAAACGAAAGCATCGCCCAGACACGCAACACCCAGGCTACAAATACAGgc                                                    >  2:4114312/1‑93 (MQ=255)
                                       acACTTCACCAGCGGTATCTTTGATCCAGTCGATGTTGTTGGAAACGAAAGCATCGCCCAGCCACGCAACACCCAGGATACAAATACAGgcgc                                                  <  1:6555945/92‑1 (MQ=255)
                                        cACTTCACCAGCGGTATCTTTGATCCAGTCGATGTTGTTGGAAACGAAAGCATCGCCCAGCCACGCAACACCCAGGATACAAATACAGGCGCt                                                 <  2:6207184/93‑1 (MQ=255)
                                         aCTTCACCAGCGGTATCTTTGATCCAGTCGATGTTGTTGGAAACGAAAGCATCGCCCAGCCACGCAACACCCAGGATACAAATACAGGCGCTc                                                <  1:6947154/93‑1 (MQ=255)
                                          cTTCACCAGCGGTATCTTTGATCCAGTCGATGTTGTTGGAAACGAAAGCATCGCCCAGCCACGCAACACCCAGGATACAAATACAGGCGCTCa                                               >  2:567631/1‑93 (MQ=255)
                                           ttCACCAGCGGTATCTTTGATCCAGTCGATGTTGTTGGAAACGAAAGCATCGCCCAGCCACGCAACACCCAGGATACAAATACAGGCGCTCAt                                              >  2:2094548/1‑93 (MQ=255)
                                            tCACCAGCGGTATCTTTGATCCAGTCGATGTTGTTGGAAACGAAAGCATCGCCCAGCCACGCAACACCCAGGATACAAATACAGGCGCTCATa                                             <  2:6937768/93‑1 (MQ=255)
                                             cACCAGCGGTATCTTTGATCCAGTCGATGTTGTTGGAAACGAAAGCATCGCCCAGCCACGCAACACCCAGGATACAAATACAGGCGCTCATAc                                            <  2:7642861/93‑1 (MQ=255)
                                              cccAGCGGTATCTTTGATCCAGTCGATGTTGTTGGAAACGAAAGCATCGCCCAGCCACGCAACACCCAGGATACAAATACAGGCGCTCATAcc                                           <  1:685460/92‑1 (MQ=255)
                                              aCCAGCGGTATCTTTGATCCAGTCGATGTTGTTGGAAACGAAAGCATCGCCCAGCCACGCAACACCCAGGATACAAATACAGGCGCTCATAcc                                           >  1:3406309/1‑93 (MQ=255)
                                              aCCAGCGGTATCTTTGATCCAGTCGATGTTGTTGGAAACGAAAGCATCGCCCAGCCACGCAACACCCAGGATACAAATACAGGCGCTCATAcc                                           <  1:6403840/93‑1 (MQ=255)
                                              aCCAGCGGTATCTTTGATCCAGTCGATGTTGTTGGAAACGAAAGCATCGCCCAGCCACGCAACACCCAGGATACAAATACAGGCGCTCATAcc                                           >  2:250490/1‑93 (MQ=255)
                                               ccAGCGGTATCTTTGATCCAGTCGATGTTGTTGGAAACGAAAGCATCGCCCAGCCACGCAACACCCAGGATACAAATACAGGCGCTCATACCt                                          >  2:189112/1‑93 (MQ=255)
                                                  gCGGTATCTTTGATCCAGTCGATGTTGTTGGAAACGAAAGCATCGCCCAGCCACGCAACACCCAGGATACACATACAGGCGCTCATACCTGCt                                       >  1:7304453/1‑93 (MQ=255)
                                                  gCGGTATCTTTGATCCAGTCGATGTTGTTGGAAACGAAAGCATCGCCCAGCCACGCAACACCCAGGATACAAATACAGGCGCTCATACCTGCt                                       <  1:4427633/93‑1 (MQ=255)
                                                  gCGGTATCTTTGATCCAGTCGATGTTGTTGGAAACGAAAGCATCGCCCAGCCACGCAACACCCAGGATACAAATACAGGCGCTCATACCTGCt                                       >  2:703932/1‑93 (MQ=255)
                                                   cGGTATCTTTGATCCAGTCGATGTTGTTGGAAACGAAAGCATCGCCCAGCCACGCAACACCCAGGATACAAATACAGGCGCTCATACCTGCtt                                      <  2:604021/93‑1 (MQ=255)
                                                    ggTATCTTTGATCCAGTCGATGTTGTTGGAAACGAAAGCATCGCCCAGCCACGCAACACCCAGGATACAAATACAGGCGCTCATACCTGCttt                                     <  2:2858955/93‑1 (MQ=255)
                                                    ggTATCTTTGATCCAGTCGATGTTGTTGGAAACGAAAGCATCGCCCAGCCACGCAACACCCAGGATACAAATACAGGCGCTCATACCTGCttt                                     <  1:49695/93‑1 (MQ=255)
                                                     gTATCTTTGATCCAGTCGATGTTGTTGGAAACGAAAGCATCGCCCAGCCACGCAACACCCAGGATACAAATACAGGCGCTCATACCTGCTTTg                                    <  1:7927085/93‑1 (MQ=255)
                                                        tCTTTGATCCAGTCGATGTTGTTGGAAACGAAAGCATCGCCCAGCCACGCAACACCCAGGATACAAATACAGGCGCTCATACCTGCTTTGCAg                                 >  1:624502/1‑93 (MQ=255)
                                                        tCTTTGATCCAGTCGATGTTGTTGGAAACGAAAGCATCGCCCAGCCACGCAACACCCAGGATACAAATACAGGCGCTCATACCTGCTTTGAAg                                 <  1:1173396/93‑1 (MQ=255)
                                                        tCTTTGATCCAGTCGATGTTGTTGGAAACGAAAGCATCGCCCAGCCACGCAACACCCAGGATACAAATACAGGCGCTCATACCTGCTTTGAAg                                 >  1:2887409/1‑93 (MQ=255)
                                                        tCTTTGATCCAGTCGATGTTGTTGGAAACGAAAGCATCGCCCAGCCACGCAACACCCAGGATACAAATACAGGCGCTCATACCTGCTTTGAAg                                 <  2:3804324/93‑1 (MQ=255)
                                                         cTTTGATCCAGTCGATGTTGTTGGAAACGAAAGCATCGCCCAGCCACGCAACACCCAGGATACAAATACAGGCGCTCATACCTGCTTTGAAgg                                <  2:7314870/93‑1 (MQ=255)
                                                         cTTTGATCCAGTCGATGTTGTTGGAAACGAAAGCATCGCCCAGCCACGCAACACCCAGGATACAAATACAGGCGCTCATACCTGCTTTGAAgg                                <  2:5881183/93‑1 (MQ=255)
                                                            tGATCCAGTCGATGTTGTTGGAAACGAAAGCATCGCCCAGCCACGCAACACCCAGGATACAAATACAGGCGCTCATACCTGCTTTGAAGGTGc                             <  1:2088749/93‑1 (MQ=255)
                                                             gATCCAGTCGATGTTGTTGGAAACGAAAGCATCGCCCAGCCACGCAACACCCAGGATACAAATACAGGCGCTCATACCTGCTTTGAAGGTGCt                            >  1:2369994/1‑93 (MQ=255)
                                                              aTCCAGTCGATGTTGTTGGAAACGAAAGCATCGCCCAGCCACGCAACACCCAGGATACAAATACAGGCGCTCATACCTGCTTTGAAGGTGCTg                           <  1:4223598/93‑1 (MQ=255)
                                                              aTCCAGTCGATGTTGTTGGAAACGAAAGCATCGCCCAGCCACGCAACACCCAGGATACAAATACAGGCGCTCATACCTGCTTTGAAGGTGCTg                           >  1:5432062/1‑93 (MQ=255)
                                                              aTCCAGTCGATGTTGTTGGAAACGAAAGCATCGCCCAGCCACGCAACACCCAGGATACAAATACAGGCGCTCATACCTGCTTTGAAGGTGCTg                           <  1:7239050/93‑1 (MQ=255)
                                                                ccAGTCGATGTTGTTGGAAACGAAAGCATCGCCCAGCCACGCACCACCCAGGATACAAATACAGGCGCTCATACCTGCTTTGAAGGTGCTGGa                         <  2:5283969/93‑1 (MQ=255)
                                                                  aGTCGATGTTGTTGGAAACGAAAGCATCGCCCAGCCACGCAACACCCAGGATACAAATACAGGCGCTCATACCTGCTTTGAAGGTGCTGGAGt                       <  2:4421991/93‑1 (MQ=255)
                                                                  aGTCGATGTTGTTGGAAACGAAAGCATCGCCCAGCCACGCAACACCCAGGATACAAATACAGGCGCTCATACCTGCTTTGAAGGTGCTGGAGt                       <  2:2019120/93‑1 (MQ=255)
                                                                   gTCGATGTTGTTGGAAACGAAAGCATCGCCCAGCCACGCAACACCCAGGATACAAATACAGGCGCTCATACCTGCTTTGAAGGTGCTGGAGtt                      <  2:6510451/93‑1 (MQ=255)
                                                                      gATGTTGTTGGAAACGAAAGCATCGCCCAGCCACGCAACACCCAGGATACAAATACAGGCGCTCATACCTGCTTTGAAGGTGCTGGAGTTGAg                   <  2:4745148/93‑1 (MQ=255)
                                                                      gATGTTGTTGGAAACGAAAGCATCGCCCAGCCACGCAACACCCAGGATACAAATACAGGCGCTCATACCTGCTTTGAAGGTGCTGGAGTTGAg                   >  2:4862054/1‑93 (MQ=255)
                                                                      gATGTTGTTGGAAACGAAAGCATCGCCCAGCCACGCAACACCCAGGATACAAATACAGGCGCTCATACCTGCTTTGAAGGTGCTGGAGTTGAg                   <  1:6705347/93‑1 (MQ=255)
                                                                      gATGTTGTTGGAAACGAAAGCATCGCCCAGCCACGCAACACCCAGGATACAAATACAGGCGCTCATACCTGCTTTGAAGGTGCTGGAGTTGAg                   >  1:4520728/1‑93 (MQ=255)
                                                                       aTGTTGTTGGAAACGAAAGCATCGCCCAGCCACGCAACACCCAGGATACAAATACAGGCGCTCATACCTGCTTTGAAGGTGCTGGAGTTGAgg                  >  1:1581487/1‑93 (MQ=255)
                                                                       aTGTTGTTGGAAACGAAAGCATCGCCCAGCCACGCAACACCCAGGATACAAATACAGGCGCTCATACCTGCTTTGAAGGTGCTGGAGTTGAgg                  <  1:272724/93‑1 (MQ=255)
                                                                       aTGTTGTTGGAAACGAAAGCATCGCCCAGCCACGCAACACCCAGGATACAAATACAGGCGCTCATACCTGCTCTGAAGGTGCTGGAGTTGAgg                  >  1:591161/1‑93 (MQ=255)
                                                                        tgttgtTGGAAACGAAAGCATCGCCCAGCCACGCAACACCCAGGATACAAATACAGGCGCTCATACCTGCTTTGAAGGTGCTGGAGTTGAggg                 <  1:3877592/93‑2 (MQ=255)
                                                                        tgttgtTGGAAACGAAAGCATCGCCCAGCCACGCAACACCCAGGATACAAATACAGGCGCTCATACCTGCTTTGAAGGTGCTGGAGTTGAggg                 >  1:7872428/1‑92 (MQ=255)
                                                                        tgttgtTGGAAACGAAAGCATCGCCCAGCCACGCAACACCCAGAATACAAATACAGGCGCTCATACCTGCTTTGAAGGTGCTGGAGTTGAggg                 >  1:4929680/1‑92 (MQ=255)
                                                                          ttgttgGAAACGAAAGCATCGCCCAGCCACGCAACACCCAGGATACAAATACAGGCGCTCATACCTGCTTTGAAGGTGCTGGAGTTGAGGGtg               <  1:1553239/93‑1 (MQ=255)
                                                                           tgttgGAAACGAAAGCATCGCCCAGCCACGCAACACCCAGGATACAAATACAGGCGCTCATACCTGCTTTGAAGGTGCTGGAGTTGAGGgtgt              <  1:310914/93‑1 (MQ=255)
                                                                             ttgGAAACGAAAGCATCGCCCAGCCACGCAACACCCAGGATACAAATACAGGCGCTCATACCTGCTTTGAAGGTGCTGGAGTTGAGGGtgttg            >  2:1513076/1‑93 (MQ=255)
                                                                               ggAAACGAAAGCATCGCCCAGCCACGCAACACCCAGGATACAAATACAGGCGCTCATACCTGCTTTGAAGGTGCTGGAGTTGAGGGTGTTGTc          <  1:2477780/93‑1 (MQ=255)
                                                                                gAAACGAAAGCATCGCCCAGCCACGCAACACCCAGGATACAAATACAGGCGCTCATACCTGCTTTGAAGGTGCTGGAGTTGAGGGTGTTGTCg         >  2:5176743/1‑93 (MQ=255)
                                                                                gAAACGAAAGCATCGCCCAGCCACGCAACACCCAGGATACAAATACAGGCGCTCATACCTGCTTTGAAGGTGCTGGAGTTGAGGGTGTTGTCg         <  1:3329742/93‑1 (MQ=255)
                                                                                  aaCGAAAGCATCGCCCAGCCACGCAACACCCAGGATACAAATACAGGCGCTCATACCTGCTTTGAAGGTGCTGGAGTTGAGGGTGTTGTCGGt       >  2:3840473/1‑93 (MQ=255)
                                                                                    cGAAAGCATCGCCCAGCCACGCAACACCCAGGATACAAATACAGGCGCTCATACCTGCTTTGAAGGTGCTGGAGTTGAGGGTGTTGTCGGTAt     <  1:1468136/93‑1 (MQ=255)
                                                                                     gAAAGCATCGCCCAGCCACGCAACACCCAGGATACAAATACAGGCGCTCATACCTGCTTTGAAGGTGCTGGAGTTGAGGGTGTTGTCGGTATc    >  2:1090538/1‑93 (MQ=255)
                                                                                     gAAAGCATCGCCCAGCCACGCAACACCCAGGATACAAATACAGGCGCTCATACCTGCTTTGAAGGTGCTGGAGTTGAGGGTGTTGTCGGTATc    >  1:4781080/1‑93 (MQ=255)
                                                                                      aaaGCATCGCCCAGCCACGCAACACCCAGGATACAAATACAGGCGCTCATACCTGCTTTGAAGGTGCTGGAGTTGAGGGTGTTGTCGGTATCg   >  2:4478009/1‑93 (MQ=255)
                                                                                          |                                                                                         
AGAAGAAGATGACGGCCAGCAGCCACGGATGACCCTGAATCACTTCACCAGCGGTATCTTTGATCCAGTCGATGTTGTTGGAAACGAAAGTATCGCCCAGCCACGCAACACCCAGGATACAAATACAGGCGCTCATACCTGCTTTGAAGGTGCTGGAGTTGAGGATGTTGTCGGTATCGA  >  NC_000913/4363337‑4363516

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output Only 200 of 235 total aligned reads displayed.
CAAAGAAGAAGATGACGGCCAGCAGCCACGGATGACCCTGAATCACTTCACCAGCGGTATCTTTGATCCAGTCGATGTTGTTGGAAACGAAAGTATCGCCCAGCCACGCAACACCCAGGATACAAATACAGGCGCTCATACCTGCTTTGAAGGTGCTGGAGTTGAGGATGTTGTCGGTATCGACTTT  >  NC_000913/4363334‑4363520
                                                                                             |                                                                                             
CAAAGAAGAAGATGACGGCCAGCAGCCACGGATGACCCTGAATCACTTCACCAGCGGTATCTTTGATCCAGTCGATGTTGTTGGAAACGAAAGC                                                                                               >  SRR4302543.5599070/1‑94 (MQ=60)
CAAAGAAGAAGATGACGGCCAGCAGCCACGGATGACCCTGAATCACTTCACCAGCGGTATCTTTGATCCAGTCGATGTTGTTGGAAACGAAAGC                                                                                               >  SRR4302543.44624/1‑94 (MQ=60)
 aacagagaaagTGACGGCCAGCAGCCACGGATGACCCTGAATCCCTTCACCAGCGTTATCTTTGATCCAGTCGATGTTGTTGGAAACGAAAGCA                                                                                              <  SRR4302543.4143117/83‑1 (MQ=60)
 AAAGAAGAAGATGACGGCCAGCAGCCACGGATGACCCTGAATCACTTCACCAGCGGTATCTTTGATCCAGTCGATGTTGTTGGAAACGAAAGCA                                                                                              <  SRR4302543.4143116/94‑1 (MQ=60)
 AAAGAAGAAGATGACGGCCAGCAGCCACGGATGACCCTGAATCACTTCACCAGCGGTATCTTTGATCCAGTCGATGTTGTTGGAAACGAAAGCA                                                                                              <  SRR4302543.3869760/94‑1 (MQ=60)
 AAAGAAGAAGATGACGGCCAGCAGCCACGGATGACCCTGAATCACTTCACCAGCGGTATCTTTGATCCAGTCGATGTTGTTGGAAACGAAAGCA                                                                                              >  SRR4302543.374716/1‑94 (MQ=60)
 AAAGAAGAAGATGACGGCCAGCAGCCACGGATGACCCTGAATCACTTCACCAGCGGTATCTTTGATCCAGTCGATGTTGTTGGAAACGAAAGCA                                                                                              >  SRR4302543.6510670/1‑94 (MQ=60)
  AAGAAGAAGATGACGGCCAGCAGCCACGGATGACCCTGAATCACTTCACCAGCGGTATCTTTGATCCAGTCGATGTTGTTGGAAACGAAAGCAT                                                                                             <  SRR4302543.3190277/94‑1 (MQ=60)
  AAGAAGAAGATGACGGCCAGCAGCCACGGATGACCCTGAATCACTTCACCAGCGGTATCTTTGATCCAGTCGATGTTGTTGGAAACGAAAGCAT                                                                                             <  SRR4302543.6749694/94‑1 (MQ=60)
   AGAAGAAGATGACGGCCAGCAGCCACGGATGACCCTGAATCACTTCACCAGCGGTATCTTTGATCCAGTCGATGTTGTTGGAAACGAAAGCATC                                                                                            >  SRR4302543.3590179/1‑94 (MQ=60)
   AGAAGAAGATGACGGCCAGCAGCCACGGATGACCCTGAATCACTTCACCAGCGGTATCTTTGATCCAGTCGATGTTGTTGGAAACGAAAGCATC                                                                                            >  SRR4302543.7432063/1‑94 (MQ=60)
   AGAAGAAGATGACGGCCAGCAGCCACGGATGACCCTGAATCACTTCACCAGCGGTATCTTTGATCCAGTCGATGTTGTTGGAAACGAAAGCATC                                                                                            <  SRR4302543.1555381/94‑1 (MQ=60)
    GAAGAAGATGACGGCCAGCAGCCACGGATGACCCTGAATCACTTCACCAGCGGTATCTTTGATCCAGTCGATGTTGTTGGAAACGAAAGCATCG                                                                                           <  SRR4302543.900892/94‑1 (MQ=60)
    GAAGAAGATGACGGCCAGCAGCCACGGATGACCCTGAATCACTTCACCAGCGGTATCTTTGATCCAGTCGATGTTGTTGGAAACGAAAGCATCG                                                                                           <  SRR4302543.7208100/94‑1 (MQ=60)
    GAAGAAGATGACGGCCAGCAGCCACGGATGACCCTGAATCACTTCACCAGCGGTATCTTTGATCCAGTCGATGTTGTTGGAAACGAAAGCATCG                                                                                           >  SRR4302543.3688557/1‑94 (MQ=60)
        AAGATGACGGCCAGCAGCCACGGATGACCCTGAATCACTTCACCAGCGGTATCTTTGATCCAGTCGATGTTGTTGGAAACGAAAGCATCGCCCA                                                                                       <  SRR4302543.4896215/94‑1 (MQ=60)
        AAGATGACGGCCAGCAGCCACGGATGACCCTGAATCACTTCACCAGCGGTATCTTTGATCCAGTCGATGTTGTTGGAAACGAAAGCATCGCCCA                                                                                       <  SRR4302543.4170639/94‑1 (MQ=60)
         AGATGACGGCCAGCAGCCACGGATGACCCTGAATCACTTCACCAGCGGTATCTTTGATCCAGTCGATGTTGTTGGAAACGAAAGCATCGCCCAG                                                                                      <  SRR4302543.3522782/94‑1 (MQ=60)
         AGATGACGGCCAGCAGCCACGGATGACCCTGAATCACTTCACCAGCGGTATCTTTGATCCAGTCGATGTTGTTGGAAACGAAAGCATCGCCCAG                                                                                      >  SRR4302543.4288170/1‑94 (MQ=60)
           ATGACGGCCAGCAGCCACGGATGACCCTGAATCACTTCACCAGCGGTATCTTTGATCCAGTCGATGTTGTTGGAAACGAAAGCATCGCCCAGCC                                                                                    <  SRR4302543.887857/94‑1 (MQ=60)
            TGACGGCCAGCAGCCACGGATGACCCTGAATCACTTCACCAGCGGTATCTTTGATCCAGTCGATGTTGTTGGAAACGAAAGCATCGCCCAGCCA                                                                                   <  SRR4302543.1576060/94‑1 (MQ=60)
                GGCCAGCAGCCACGGATGACCCTGAATCACTTCACCAGCGGTATCTTTGATCCAGTCGATGTTGTTGGAAACGAAAGCATCGCCCAGCCACGCA                                                                               >  SRR4302543.5081077/1‑94 (MQ=60)
                GGCCAGCAGCCACGGATGACCCTGAATCACTTCACCAGCGGTATCTTTGATCCAGTCGATGTTGTTGGAAACGAAAGCATCGCCCAGCCACGCA                                                                               >  SRR4302543.8001608/1‑94 (MQ=60)
                 GCCAGCAGCCACGGATGACCCTGAATCACTTCACCAGCGGTATCTTTGATCCAGTCGATGTTGTTGGAAACGAAAGCATCGCCCAGCCACGCAA                                                                              >  SRR4302543.3880543/1‑94 (MQ=60)
                 GCCAGCAGCCACGGATGACCCTGAATCACTTCACCAGCGGTATCTTTGATCCAGTCGATGTTGTTGGAAACGAAAGCATCGCCCAGCCACGCAA                                                                              <  SRR4302543.7335174/94‑1 (MQ=60)
                  CCAGCAGCCACGGATGACCCTGAATCACTTCACCAGCGGTATCTTTGATCCAGTCGATGTTGTTGGAAACGAAAGCATCGCCCAGCCACGCAAC                                                                             <  SRR4302543.1353986/94‑1 (MQ=60)
                  CCAGCAGCCACGGATGACCCTGAATCACTTCACCAGCGGTATCTTTGATCCAGTCGATGTTGTTGGAAACGAAAGCATCGCCCAGCCACGCAAC                                                                             >  SRR4302543.4857316/1‑94 (MQ=60)
                  CAAGCAGCCACGGATGACCCTGAATCACTTCACCAGCGGTATCTTTGATCCAGTCGATGTTGTTGGAAACGAAAGCATCCCCCAGCCATGCAAC                                                                             >  SRR4302543.1268813/1‑94 (MQ=60)
                   CAGCAGCCACGGATGACCCTGAATCACTTCACCAGCGGTATCTTTGATCCAGTCGATGTTGTTGGAAACGAACGCATCGCCCAGCCACGCCACA                                                                            >  SRR4302543.3020341/1‑94 (MQ=60)
                   CAGCAGCCACGGATGACCCTGAATCACTTCACCAGCGGTATCTTTGATCCAGTCGATGTTGTTGGAAACGAAAGCATCGCCCAGCCACGCAACA                                                                            >  SRR4302543.7384016/1‑94 (MQ=60)
                   CAGCAGCCACGGATGACCCTGAATCACTTCACCAGCGGTATCTTTGATCCAGTCGATGTTGTTGGAAACGAAAGCATCGCCCAGCCACGCAACA                                                                            >  SRR4302543.2319141/1‑94 (MQ=60)
                   CAGCAGCCACGGATGACCCTGAATCACTTCACCAGCGGTATCTTTGATCCAGTCGATGTTGTTGGAAACGAAAGCATCGCCCAGCCACGCAACA                                                                            <  SRR4302543.289311/94‑1 (MQ=60)
                   CAGCAGCCACGGATGACCCTGAATCACTTCACCAGCGGTATCTTTGATCCAGTCGATGTTGTTGGAAACGAAAGCATCGCCCAGCCACGCAACA                                                                            >  SRR4302543.6181912/1‑94 (MQ=60)
                    AGCAGCCACGGATGACCCTGAATCACTTCACCAGCGGTATCTTTGATCCAGTCGATGTTGTTGGAAACGAAAGCATCGCCCAGCCACGCAACAC                                                                           <  SRR4302543.5922019/94‑1 (MQ=60)
                    AGCAGCCACGGATGACCCTGAATCACTTCACCAGCGGTATCTTTGATCCAGTCGATGTTGTTGGAAACGAAAGCATCGCCCAGCCACGCAACAC                                                                           <  SRR4302543.1653535/94‑1 (MQ=60)
                    AGCAGCCACGGATGACCCTGAATCACTTCACCAGCGGTATCTTTGATCCAGTCGATGTTGTTGGAAACGAAAGCATCGCCCAGCCACGCAACAC                                                                           >  SRR4302543.7178891/1‑94 (MQ=60)
                    AGCAGCCACGGATGACCCTGAATCACTTCACCAGCGGTATCTTTGATCCAGTCGATGTTGTTGGAAACGAAAGCATCGCCCAGCCACGCAACAC                                                                           >  SRR4302543.2239494/1‑94 (MQ=60)
                     GCAGCCACGGATGACCCTGAATCACTTCACCAGCGGTATCTTTGATCCAGTCGATGTTGTTGGAAACGAAAGCATCGCCCAGCCACGCAACACC                                                                          <  SRR4302543.6346408/94‑1 (MQ=60)
                      CAGCCACGGATGACCCTGAATCACTTCACCAGCGGTATCTTTGATCCAGTCGATGTTGTTGGAAACGAAAGCATCGCCCAGCCACGCAACACCC                                                                         <  SRR4302543.7951787/94‑1 (MQ=60)
                      CAGCCACGGATGACCCTGAATCACTTCACCAGCGGTATCTTTGATCCAGTCGATGTTGTTGGAAACGAAAGCATCGCCCAGCCACGCAACACCC                                                                         <  SRR4302543.5860778/94‑1 (MQ=60)
                      CAGCCACGGATGACCCTGAATCACTTCACCAGCGGTATCTTTGATCCAGTCGATGTTGTTGGAAACGAAAGCATCGCCCAGCCACGCAACACCC                                                                         <  SRR4302543.7683952/94‑1 (MQ=60)
                      CAGCCACGGATGACCCTGAATCACTTCACCAGCGGTATCTTTGATCCAGTCGATGTTGTTGGAAACGAAAGCATCGCCCAGCCACGCAACACCC                                                                         <  SRR4302543.324196/94‑1 (MQ=60)
                       AGCCACGGATGACCCTGAATCACTTCACCAGCGGTATCTTTGATCCAGTCGATGTTGTTGGAAACGAAAGCATCGCCCAGCCACGCAACACCCA                                                                        <  SRR4302543.3591437/94‑1 (MQ=60)
                       AGCCACGGATGACCCTGAATCACTTCACCAGCGGTATCTTTGATCCAGTCGATGTTGTTGGAAACGAAAGCATCGCCCAGCCACGCAACACCCA                                                                        <  SRR4302543.3486948/94‑1 (MQ=60)
                       AGCCACGGATGACCCTGAATCACTTCACCAGCGGTATCTTTGATCCAGTCGATGTTGTTGGAAACGAAAGCATCGCCCAGCCACGCAACACCCA                                                                        <  SRR4302543.238169/94‑1 (MQ=60)
                       AGCCACGGATGACCCTGAATCACTTCACCAGCGGTATCTTTGATCCAGTCGATGTTGTTGGAAACGAAAGCATCGCCCAGCCACGCAACACCCA                                                                        >  SRR4302543.6823299/1‑94 (MQ=60)
                        GCCACGGATGACCCTGAATCACTTCACCAGCGGTATCTTTGATCCAGTCGATGTTGTTGGAAACGAAAGCATCGCCCAGCCACGCAACACCCAG                                                                       <  SRR4302543.7994428/94‑1 (MQ=60)
                        GCCACGGATGACCCTGAATCACTTCACCAGCGGTATCTTTGATCCAGTCGATGTTGTTGGAAACGAAAGCATCGCCCAGCCACGCAACACCCAG                                                                       <  SRR4302543.6590157/94‑1 (MQ=60)
                         CCACGGATGACCCTGAATCTCTTCACCAGCGGTATCTTTGATCCAGTCGATGTTGTTGGAAACGAAAGCATCGCCCAGCCACGCAACACCCAGG                                                                      <  SRR4302543.7191957/94‑1 (MQ=60)
                         CCACGGATGACCCTGAATCACTTCACCAGCGGTATCTTTGATCCAGTCGATGTTGTTGGAAACGAAAGCATCGCCCAGCCACGCAACACCCAGG                                                                      >  SRR4302543.7977653/1‑94 (MQ=60)
                          CACGGATGACCCTGAATCACTTCACCAGCGGTATCTTTGATCCAGTCGATGTTGTTGGAAACGAAAGCATCGCCCAGCCACGCAACACCCAGGA                                                                     >  SRR4302543.1923117/1‑94 (MQ=60)
                          CACGGATGACCCTGAATCACTTCACCAGCGGTATCTTTGATCCAGTCGATGTTGTTGGAAACGAAAGCATCGCCCAGCCACGCAACACCCAGGA                                                                     >  SRR4302543.5206899/1‑94 (MQ=60)
                          CACGGATGACCCTGAATCACTTCACCAGCGGTATCTTTGATCCAGTCGATGTTGTTGGAAACGAAAGCATCGCCCAGCCACGCAACACCCAGGA                                                                     <  SRR4302543.4975687/94‑1 (MQ=60)
                           ACGGATGACCCTGAATCACTTCACCAGCGGTATCTTTGATCCAGTCGATGTTGTTGGAAACGAAAGCATCGCCCAGCCACGCAACACCCAGGAT                                                                    >  SRR4302543.65015/1‑94 (MQ=60)
                            CGGATGACCCTGAATCACTTCACCAGCGGTATCTTTGATCCAGTCGATGTTGTTGGAAACGAAAGCATCGCCCAGCCACGCAACACCCAGGATA                                                                   <  SRR4302543.5696324/94‑1 (MQ=60)
                            CGGATGACCCTGAATCACTTCACCAGCGGTATCTTTGATCCAGTCGATGTTGTTGGAAACGAAAGCATCGCCCAGCCACGCAACACCCAGGATA                                                                   <  SRR4302543.6930688/94‑1 (MQ=60)
                             GGATGACCCTGAATCACTTCACCAGCGGTATCTTTGATCCAGTCGATGTTGTTGGAAACGAAAGCATCGCCCAGCCACGCAACACCCAGGATAC                                                                  >  SRR4302543.1805420/1‑94 (MQ=60)
                              GATGACCCTGAATCACTTCACCAGCGGTATCTTTGATCCAGTCGATGTTGTTGGAAACGAAAGCATCGCCCAGCCACGCAACACCCAGGATACA                                                                 <  SRR4302543.1802226/94‑1 (MQ=60)
                               ATGACCCTGAATCACTTCACCAGCGGTATCTTTGATCCAGTCGATGTTGTTGGAAACGAAAGCATCGCCCAGCCACGCAACACCCAGGATACAA                                                                >  SRR4302543.2529083/1‑94 (MQ=60)
                               ATGACCCTGAATCACTTCACCAGCGGTATCTTTGATCCAGTCGATGTTGTTGGAAACGAAAGCATCGCCCAGCCACGCAACACCCAGGATACAA                                                                <  SRR4302543.3916901/94‑1 (MQ=60)
                                 GACCCTGAATCACTTCACCAGCGGTATCTTTGATCCAGTCGATGTTGTTGGAAACGAAAGCATCGCCCAGCCACGCAACACCCAGGATACAAAT                                                              <  SRR4302543.3344879/94‑1 (MQ=60)
                                 GACCCTGAATCACTTCACCAGCGGTATCTTTGATCCAGTCGATGTTGTTGGAAACGAAAGCATCGCCCAGCCACGCAACACCCAGGATACAAAT                                                              <  SRR4302543.3344880/94‑1 (MQ=60)
                                  ACCCTGAATCACTTCACCAGCGGTATCTTTGATCCAGTCGATGTTGTTGGAAACGAAAGCATCGCCCAGCCACGCAACACCCAGGATACAAATA                                                             >  SRR4302543.1905587/1‑94 (MQ=60)
                                  ACCCTGAATCACTTCACCAGCGGTATCTTTGATCCAGTCGATGTTGTTGGAAACGAAAGCATCGCCCAGCCACGCAACACCCAGGATACAAATA                                                             <  SRR4302543.6687211/94‑1 (MQ=60)
                                  ACCCTGAATCACTTCACCAGCGGTATCTTTGATCCAGTCGATGTTGTTGGAAACGAAAGCATCGCCCAGCCACGCAACACCCAGGATACAAATA                                                             >  SRR4302543.4673994/1‑94 (MQ=60)
                                   CCCTGAATCACTTCACCAGCGGTATCTTTGATCCAGTCGATGTTGTTGGAAACGAAAGCATCGCCCAGCCACGCAACACCCAGGATACAAATAC                                                            <  SRR4302543.1314030/94‑1 (MQ=60)
                                   CCCTGAATCACTTCACCAGCGGTATCTTTGATCCAGTCGATGTTGTTGGAAACGAAAGCATCGCCCAGCCACGCAACACCCAGGATACAAATAC                                                            >  SRR4302543.1450877/1‑94 (MQ=60)
                                   CCCTGAATCACTTCACCAGCGGTATCTTTGATCCAGTCGATGTTGTTGGAAACGAAAGCATCGCCCAGCCACGCAACACCCAGGATACAAATAC                                                            >  SRR4302543.4795939/1‑94 (MQ=60)
                                   CCCTGAATCACTTCACCAGCGGTATCTTTGATCCAGTCGATGTTGTTGGAAACGAAAGCATCGCCCAGCCACGCAACACCCAGGATACAAATAC                                                            >  SRR4302543.6556911/1‑94 (MQ=60)
                                   CCCTGAATCACTTCACCAGCGGTATCTTTGATCCAGTCGATGTTGTTGGAAACGAAAGCATCGCCCAGCCACGCAACACCCAGGATACAAATAC                                                            <  SRR4302543.2833065/94‑1 (MQ=60)
                                   CCCTGAATCACTTCACCAGCGGTATCTTTGATCCAGTCGATGTTGTTGGAAACGAAAGCATCGCCCAGCCACGCAACACCCAGGATACAAATAC                                                            <  SRR4302543.6158894/94‑1 (MQ=60)
                                    CCTGAATCACTTCACCAGCGGTATCTTTGATCCAGTCGATGTTGTTGGAAACGAAAGCATCGCCCAGCCACGCAACACCCAGGATACAAATACA                                                           >  SRR4302543.7426015/1‑94 (MQ=60)
                                     CTGAATCACTTCACCAGCGGTATCTTTGATCCAGTCGATGTTGTTGGAAACGAAAGCATCGCCCAGCCACGCAACACCCAGGATACAAATACAG                                                          >  SRR4302543.3740427/1‑94 (MQ=60)
                                     CTGAATCACTTCACCAGCGGTATCTTTGATCCAGTCGATGTTGTTGGAAACGAAAGCATCGCCCAGCCACGCAACACCCAGGATACAAATACAG                                                          <  SRR4302543.1548378/94‑1 (MQ=60)
                                     CTGAATCACTTCACCAGCGGTATCTTTGATCCAGTCGATGTTGTTGGAAACGAAAGCATCGCCCAGCCACGCAACACCCAGGATACAAATACAG                                                          <  SRR4302543.5345062/94‑1 (MQ=60)
                                     CTGAATCACTTCACCAGCGGTATCTTTGATCCAGTCGATGTTGTTGGAAACGAAAGCATCGCCCAGCCACGCAACACCCAGGATACAAATACAG                                                          <  SRR4302543.6926282/94‑1 (MQ=60)
                                      TGAATCACTTCACCAGCGGTATCTTTGATCCAGTCGATGTTGTTGGAAACGAAAGCATCGCCCAGCCACGCACCACCCAGGATACAAATACAGC                                                         >  SRR4302543.5661634/1‑94 (MQ=60)
                                      TGAATCACTTCACCAGCGGTATCTTTGATCCAGTCGATGTTGTTGGAAACGAAAGCATCGCCCAGCCACGCAACACCCAGGATACAAATACAGG                                                         <  SRR4302543.7323619/94‑1 (MQ=60)
                                      TGAATCACTTCACCAGCGGTATCTTTGATCCAGTCGATGTTGTTGGAAACGAAAGCATCGCCCAGCCACGCAACACCCAGGATACAAATACAGG                                                         <  SRR4302543.5288253/94‑1 (MQ=60)
                                      TGAATCACTTCACCAGCGGTATCTTTGATCCAGTCGATGTTGTTGGAAACGAAAGCATCGCCCAGCCACGCAACACCCAGGATACAAATACAGG                                                         >  SRR4302543.12590/1‑94 (MQ=60)
                                      TGAATCACTTCACCAGCGGTATCTTTGATCCAGTCGATGTTGTTGGAAACGAAAGCATCGCCCAGCCACGCAACACCCAGGATACAAATACAGG                                                         <  SRR4302543.4160120/94‑1 (MQ=60)
                                      TGAATCACTTCACCAGCGGTATCTTTGATCCAGTCGATGTTGTTGGAAACGAAAGCATCGCCCAGCCACGCAACACCCAGGATACAAATACAGG                                                         >  SRR4302543.5661635/1‑94 (MQ=60)
                                      TGAATCACTTCACCAGCGGTATCTTTGATCCAGTCGATGTTGTTGGAAACGAAAGCATCGCCCAGCCACGCAACACCCAGGATACAAATACAGG                                                         >  SRR4302543.4399530/1‑94 (MQ=60)
                                      TGAATCACTTCACCAGCGGTATCTTTGATCCAGTCGATGTTGTTGGAAACGAAAGCATCGCCCAGCCACGCAACACCCAGGATACAAATACAGG                                                         <  SRR4302543.1948936/94‑1 (MQ=60)
                                      TGAATCACTTCACCAGCGGTATCTTTGATCCAGTCGATGTTGTTGGAAACGAAAGCATCGCCCAGCCACGCAACACCCAGGATACAAATACAGG                                                         >  SRR4302543.2016139/1‑94 (MQ=60)
                                      TGAATCACTTCACCAGCGGTATCTTTGATCCAGTCGATGTTGTTGGAAACGAAAGCATCGCCCAGCCACGCAACACCCAGGATACAAATACAGG                                                         >  SRR4302543.1383973/1‑94 (MQ=60)
                                       GAATCACTTCACCAGCGGTATCTTTGATCCAGTCGATGTTGTTGGAAACGAAAGCATCGCCCAGCCACGCAACACCCAGGATACAAATACAGGC                                                        >  SRR4302543.865641/1‑94 (MQ=60)
                                       GAATCACTTCACCAGCGGTATCTTTGATCCAGTCGATGTTGTTGGAAACGAAAGCATCGCCCAGCCACGCAACACCCAGGATACAAATACAGGC                                                        <  SRR4302543.5392388/94‑1 (MQ=60)
                                        AATCACTTCACCAGCGGTATCTTTGATCCAGTCGATGTTGTTGGAAACGAAAGCATCGCCCAGCCACGCAACACCCAGGATACAAATACAGGCG                                                       <  SRR4302543.2902008/94‑1 (MQ=60)
                                        AATCACTTCACCAGCGGTATCTTTGATCCAGTCGATGTTGTTGGAAACGAAAGCATCGCCCAGCCACGCAACACCCAGGATACAAATACAGGCG                                                       <  SRR4302543.4519711/94‑1 (MQ=60)
                                         ATCACTTCACCAGCGGTATCTTTGATCCAGTCGATGTTGTTGGAAACGAAAGCATCGCCCAGCCACGCAACACCCAGGATACAAATACAGGCGC                                                      <  SRR4302543.723850/94‑1 (MQ=60)
                                          TCACTTCACCAGCGGTATCTTTGATCCAGTCGATGTTGTTGGAAACGAAAGCATCGCCCAGCCACGCAACACCCAGGATACAAATACAGGCGCT                                                     <  SRR4302543.1439520/94‑1 (MQ=60)
                                          TCACTTCACCAGCGGTATCTTTGATCCAGTCGATGTTGTTGGAAACGAAAGCATCGCCCAGCCACGCAACACCCAGGATACAAATACAGGCGCT                                                     >  SRR4302543.4430252/1‑94 (MQ=60)
                                          TCACTTCACCAGCGGTATCTTTGATCCAGTCGATGTTGTTGGAAACGAAAGCATCGCCCAGCCACGCAACACCCAGGATACAAATACAGGCGCC                                                     >  SRR4302543.4344781/1‑94 (MQ=60)
                                          ACACTTCACCAGCGGTATCTTTGATCCAGTCGATGTTGTTGGAAACGAAAGCATCGCCCAGCCACGCAACACCCAGGATACAAATACAGGCGCT                                                     <  SRR4302543.6617639/94‑1 (MQ=60)
                                           CACTTCACCAGCGGTATCTTTGATCCAGTCGATGTTGTTGGAAACGAAAGCATCGCCCAGCCACGCAACACCCAGGATACAAATACAGGCGCTC                                                    >  SRR4302543.2402932/1‑94 (MQ=60)
                                           CACTTCACCAGCGGTATCTTTGATCCAGTCGATGTTGTTGGAAACGAAAGCATCGCCCAGCCACGCAACACCCAGGATACAAATACAGGCGCTC                                                    >  SRR4302543.2900100/1‑94 (MQ=60)
                                           CACTTCACCAGCGGTATCTTTGATCCAGTCGATGTTGTTGGAAACGAAAGCATCGCCCAGCCACGCAACACCCAGGATACAAATACAGGCGCTC                                                    <  SRR4302543.1600463/94‑1 (MQ=60)
                                           CACTTCACCAGCGGTATCTTTGATCCAGTCGATGTTGTTGGAAACGAAAGCATCGCCCAGCCACGCAACACCCAGGATACAAATACAGGCGCTC                                                    >  SRR4302543.1856959/1‑94 (MQ=60)
                                            ACTTCACCAGCGGTATCTTTGATCCAGTCGATGTTGTTGGAAACGAAAGCATCGCCCAGCCACGCAACACCCAGGATACAAATACAGGCGCTCA                                                   <  SRR4302543.7012515/94‑1 (MQ=60)
                                            ACTTCACCAGCGGTATCTTTGATCCAGTCGATGTTGTTGGAAACGAAAGCATCGCCCAGCCACGCAACACCCAGGATACAAATACAGGCGCTCA                                                   <  SRR4302543.3917894/94‑1 (MQ=60)
                                             CTTCACCAGCGGTATCTTTGATCCAGTCGATGTTGTTGGAAACGAAAGCATCGCCCAGCCACGCAACACCCAGGATACAAATACAGGCGCTCAT                                                  <  SRR4302543.3233235/94‑1 (MQ=60)
                                             CTTCACCAGCGGTATCTTTGATCCAGTCGATGTTGTTGGAAACGAAAGCATCGCCCAGCCACGCAACACCCAGGATACAAATACAGGCGCTCAT                                                  >  SRR4302543.2392928/1‑94 (MQ=60)
                                             CTTCACCAGCGGTATCTTTGATCCAGTCGATGTTGTTGGAAACGAAAGCATCGCCCAGCCACGCAACACCCAGGATACAAATACAGGCGCTCAT                                                  <  SRR4302543.2537749/94‑1 (MQ=60)
                                             CTTCACCAGCGGAATCTTTGATCCAGTCGATGTTGTTGGAAACGAAAGAATCGCACAGCCACGCAACACCCAGGATACAAATACAGGCGATCAT                                                  >  SRR4302543.3770662/1‑94 (MQ=60)
                                               TCACCAGCGGTATCTTTGATCCAGTCGATGTTGTTGGAAACGAAAGCATCGCCCAGCCACGCAACACCCAGGATACAAATACAGGCGCTCATAC                                                <  SRR4302543.2935809/94‑1 (MQ=60)
                                               TCACCAGCGGTATCTTTGATCCAGTCGATGCTGTTGGAAACGAAAGCATCGCCCAGCCACGCAACACCCAGGATACAAATACAGGCGCTCATAC                                                <  SRR4302543.5896599/94‑1 (MQ=60)
                                                CACCAGCGGTATCTTTGATCCAGTCGATGTTGTTGGAAACGAAAGCATCGCCCAGCCACGCAACACCCAGGATACAAATACAGGCGCTCATACC                                               >  SRR4302543.4244516/1‑94 (MQ=60)
                                                 CCCAGCGGTATCTTTGATCCAGTCGATGTTGTTGGAAACGAAAGCATCGCCCAGCCACGCAACACCCAGGATACAAATACAGGCGCTCATACCT                                              <  SRR4302543.1593713/94‑1 (MQ=60)
                                                 CCCAGCGGTATCTTTGATCCAGTCGATGTTGTTGGAAACGAAAGCATCGCCCAGCCACGCAACACCCAGGATACAAATACAGGCGCTCATACCT                                              <  SRR4302543.691774/94‑1 (MQ=60)
                                                 ACCAGCGGTATCTTTGATCCAGTCGATGTTGTTGGAAACGAAAGCATCGCCCAGCCACGCAACACCCAGGATACAAATACAGGCGCTCATACCT                                              <  SRR4302543.500516/94‑1 (MQ=60)
                                                 ACCAGCGGTATCTTTGATCCAGTCGATGTTGTTGGAAACGAAAGCATCGCCCAGCCACGCAACACCCAGGATACAAATACAGGCGCTCATACCT                                              <  SRR4302543.4145130/94‑1 (MQ=60)
                                                   CAGCGGTATCTTTGATCCAGTCGATGTTGTTGGAAACGAAAGCATCGCCCAGCCACGCAACACCCAGGATACAAATACAGGCGCTCATACCTGC                                            <  SRR4302543.7629407/94‑1 (MQ=60)
                                                   CAGCGGTATCTTTGATCCAGTCGATGTTGTTGGAAACGAAAGCATCGCCCAGCCACGCAACACCCAGGATACAAATACACGCGCTCATACCTGC                                            >  SRR4302543.5689806/1‑94 (MQ=60)
                                                    AGCGGTATCTTTGATCCAGTCGATGTTGTTGGAAACGAAAGCATCGCCCAGCCACGCAACACCCAGGATACACATACAGGCGCTCATACCTGCT                                           >  SRR4302543.7374895/1‑94 (MQ=60)
                                                     GCGGTATCTTTGATCCAGTCGATGTTGTTGGAAACGAAAGCATCGCCCAGCCACGCAACACCCAGGATACAAATACAGGCGCTCATACCTGCTT                                          <  SRR4302543.4467749/94‑1 (MQ=60)
                                                      CGGTATCTTTGATCCAGTCGATGTTGTTGGAAACGAAAGCATCGCCCAGCCACGCAACACCCAGGATACAAATACAGGCGCTCATACCTGCTTT                                         >  SRR4302543.7700037/1‑94 (MQ=60)
                                                      CGGTATCTTTGATCCAGTCGATGTTGTTGGAAACGAAAGCATCGCCCAGCCACGCAACACCCAGGATACAAATACAGGCGCTCATACCTGCTTT                                         >  SRR4302543.2113394/1‑94 (MQ=60)
                                                      CGGTATCTTTGATCCAGTCGATGTTGTTGGAAACGAAAGCATCGCCCAGCCACGCAACACCCAGGATACAAATACAGGCGCTCATACCTGCTTT                                         <  SRR4302543.4904545/94‑1 (MQ=60)
                                                       GGTATCTTTGATCCAGTCGATGTTGTTGGAAACGAAAGCATCGCCCAGCCACGCAACACCCAGGATCCAAATACAGGCGCTCATACCTGCTTTG                                        >  SRR4302543.410385/1‑94 (MQ=60)
                                                       GGTATCTTTGATCCAGTCGATGTTGTTGGAAACGAAAGCATCGCCCAGCCACGCAACACCCAGGATACAAATACAGGCGCTCATACCTGCTTTG                                        >  SRR4302543.4556453/1‑94 (MQ=60)
                                                       GGTATCTTTGATCCAGTCGATGTTGTTGGAAACGAAAGCATCGCCCAGCCACGCAACACCCAGGATACAAATACAGGCGCTCATACCTGCTTTG                                        >  SRR4302543.3463157/1‑94 (MQ=60)
                                                        GTATCTTTGATCCAGTCGATGTTGTTGGAAACGAAAGCATCGCCCAGCCACGCAACACCCAGGATACAAATACAGGCGCTCATACCTGCTTTGC                                       >  SRR4302543.277981/1‑94 (MQ=60)
                                                        GTATCTTTGATCCAGTCGATGTTGTTGGAAACGAAAGCATCGCCCAGCCACGCAACACCCAGGATACAAATACAGGCGCTCATACCTGCTTTGA                                       >  SRR4302543.7635993/1‑94 (MQ=60)
                                                        GTATCTTTGATCCAGTCGATGTTGTTGGAAACGAAAGCATCGCCCAGCCACGCAACACCCAGGATACAAATACAGGCGCTCATACCTGCTTTGA                                       >  SRR4302543.6337487/1‑94 (MQ=60)
                                                        GTATCTTTGATCCAGTCGATGTTGTTGGAAACGAAAGCATCGCCCAGCCACGCAACACCCAGGATACAAATACAGGCGCTCATACCTGCTTTGA                                       >  SRR4302543.1790428/1‑94 (MQ=60)
                                                          ATCTTTGATCCAGTCGATGTTGTTGGAAACGAAAGCATCGCCCAGCCACGCAACACCCAGGATACAAATACAGGCGCTCATACCTGCTTTGCAG                                     >  SRR4302543.630218/1‑94 (MQ=60)
                                                          ATCTTTGATCCAGTCGATGTTGTTGGAAACGAAAGCATCGCCCAGCCACGCAACACCCAGGATACAAATACAGGCGCTCATACCTGCTTTGAAG                                     >  SRR4302543.2913682/1‑94 (MQ=60)
                                                          ATCTTTGATCCAGTCGATGTTGTTGGAAACGAAAGCATCGCCCAGCCACGCAACACCCAGGATACAAATACAGGCGCTCATACCTGCTTTGAAG                                     >  SRR4302543.1392450/1‑94 (MQ=60)
                                                           TCTTTGATCCAGTCGATGTTGTTGGAAACGAAAGCATCGCCCAGCCACGCAACACCCAGGATACAAATACAGGCGCTCATACCTGCTTTGAAGG                                    <  SRR4302543.672473/94‑1 (MQ=60)
                                                           TCTTTGATCCAGTCGATGTTGTTGGAAACGAAAGCATCGCCCAGCCACGCAACACCCAGGATACAAATACAGGCGCTCATACCTGCTTTGAAGG                                    <  SRR4302543.7282210/94‑1 (MQ=60)
                                                           TCTTTGATCCAGTCGATGTTGTTGGAAACGAAAGCATCGCCCAGCCACGCAACACCCAGGATACAAATACAGGCGCTCATACCTGCTTTGAAGG                                    <  SRR4302543.1184033/94‑1 (MQ=60)
                                                            CTTTGATCCAGTCGATGTTGTTGGAAACGAAAGCATCGCCCAGCCACGCAACACCCAGGATACAAATACAGGCGCTCATACCTGCTTTGAAGGT                                   >  SRR4302543.4866831/1‑94 (MQ=60)
                                                               TGATCCAGTCGATGTTGTTGGAAACGAAAGCATCGCCCAGCCACGCAACACCCAGGATACAAATACAGGCGCTCATACCTGCTTTGAAGGTGCT                                <  SRR4302543.2107412/94‑1 (MQ=60)
                                                               TGATCCAGTCGATGTTGTTGGAAACGAAAGCATCGCCCAGCCACGCAACACCCAGGATACAAATACAGGCGCTCATACCTGCTTTGAAGGTGCT                                >  SRR4302543.2391473/1‑94 (MQ=60)
                                                                TATCCAGTCGATGTTGTTGGAAACGAAAGCATCGCCCAGCCACGCAACACCCAGGATACAAATACAGGCGCTCATACCTGCTTTGAAGGTGCTG                               <  SRR4302543.1976054/94‑1 (MQ=60)
                                                                GATCCAGTCGATGTTGTTGGAAACGAAAGCATCGCCCAGCCACGCAACACCCAGGATACAAATACAGGCGCTCATACCTGCTTTGAAGGTGCTG                               >  SRR4302543.5481417/1‑94 (MQ=60)
                                                                 ATCCAGTCGATGTTGTTGGAAACGAAAGCATCGCCCAGCCACGCAACACCCAGGATACAAATACAGGCGCTCATACCTGCTTTGAAGGTGCTGG                              <  SRR4302543.4261898/94‑1 (MQ=60)
                                                                 ATCCAGTCGATGTTGTTGGAAACGAAAGCATCGCCCAGCCACGCAACACCCAGGATACAAATACAGGCGCTCATACCTGCTTTGAAGGTGCTGG                              <  SRR4302543.7309126/94‑1 (MQ=60)
                                                                 ATCCAGTCGATGTTGTTGGAAACGAAAGCATCGCCCAGCCACGCAACACCCAGGATACAAATACAGGCGCTCATACCTGCTTTGAAGGTGCTGG                              >  SRR4302543.3137135/1‑94 (MQ=60)
                                                                 ATCCAGTCGATGTTGTTGGAAACGAAAGCATCGCCCAGCCACGCAACACCCAGGATACAAATACAGGCGCTCATACCTGCTTTGAAGGTGCTGG                              <  SRR4302543.1922616/94‑1 (MQ=60)
                                                                   CCAGTCGATGTTGTTGGAAACGAAAGCATCGCCCAGCCACGCAACACCCAGGATACAAATACAGGCGCTCATACCTGCTTTGAAGGTGCTGGAG                            <  SRR4302543.681692/94‑1 (MQ=60)
                                                                    CAGTCGATGTTGTTGGAAACGAAAGCATCGCCCAGCCACGCAACACCCAGGATACAAATACAGGCGCTCATACCTGCTTTGAAGGTGCTGGAGT                           >  SRR4302543.4877255/1‑94 (MQ=60)
                                                                    CAGTCGATGTTGTTGGAAACGAAAGCATCGCCCAGCCACGCAACACCCAGGATACAAATACAGGCGCTCATACCTGCTTTGAAGGTGCTGGAGT                           >  SRR4302543.5960049/1‑94 (MQ=60)
                                                                    CAGTCGATGTTGTTGGAAACGAAAGCATCGCCCAGCCACGCAACACCCAGGATACAAATACAGGCGCTCATACCTGCTTTGAAGGTGCTGGAGT                           <  SRR4302543.2849193/94‑1 (MQ=60)
                                                                    CAGTCGATGTTGTTGGAAACGAAAGCATCGCCCAGCCACGCAACACCCAGGATACAAATACAGGCGCTCATACCTGCTTTGAAGGTGCTGGAGT                           >  SRR4302543.336927/1‑94 (MQ=60)
                                                                    CAGTCGATGTTGTTGGAAACGAAAGCATCGCCCAGCCACGCAACACCCAGGATACAAATACAGGCGCTCATACCTGCTTTGAAGGTGCTGGAGT                           <  SRR4302543.1609791/94‑1 (MQ=60)
                                                                     AGTCGATGTTGTTGGAAACGAAAGCATCGCCCAGCCACGCAACACCCAGGATACAAATACAGGCGCTCATACCTGCTTTGAAGGTGCTGGAGTT                          <  SRR4302543.342843/94‑1 (MQ=60)
                                                                     AGTCGATGTTGTTGGAAACGAAAGCATCGCCCAGCCACGCAACACCCAGGATACAAATACAGGCGCTCATACCTGCTTTGAAGGTGCTGGAGTT                          >  SRR4302543.7008662/1‑94 (MQ=60)
                                                                     AGTCGATGTTGTTGGAAACGAAAGCATCGCCCAGCCACGCAACACCCAGGATACAAATACAGGCGCTCATACCTGCTTTGAAGGTGCTGGAGTT                          <  SRR4302543.5216710/94‑1 (MQ=60)
                                                                      GTCGATGTTGTTGGAAACGAAAGCATCGCCGAGCCACGCAACACCCAGGATACAAATACAGGCGCTCATACCTGCTTTGAAGGTGCTGGAGGTG                         >  SRR4302543.7392522/1‑94 (MQ=60)
                                                                      GTCGATGTTGTTGGAAACGAAAGCATCGCCCAGCCACGCAACACCCAGGATACAAATACAGGCGCTCATACCTGCTTTGAAGGTGCTGGAGTTG                         <  SRR4302543.5100822/94‑1 (MQ=60)
                                                                      GTCGATGTTGTTGGAAACGAAAGCATCGCCCAGCCACGCAACACCCAGGATACAAATACAGGCGCTCATACCTGCTTTGAAGGTGCTGGAGTTG                         <  SRR4302543.4042647/94‑1 (MQ=60)
                                                                      GTCGATGTTGTTGGAAACGAAAGCATCGCCCAGCCACGCAACACCCAGGATACAAATACAGGCGCTCATACCTGCTTTGAAGGTGCTGGAGTTG                         <  SRR4302543.2169045/94‑1 (MQ=60)
                                                                      GTCGATGTTGTTGGAAACGAAAGCATCGCCCAGCCACGCAACACCCAGGATACAAATACAGGCGCTCAAACCTGCTTTGAAGGTGCTGGAGTTG                         <  SRR4302543.5222452/94‑1 (MQ=60)
                                                                       TCGATGTTGTTGCAAACGAAAGCATCGCCCAGCCACGCAACACCCAGGATACAAATACAGGCGCTCATACCTGCTTTGAAGGTGCTGGAGTTGA                        <  SRR4302543.631839/94‑1 (MQ=60)
                                                                        CGATGTTGTTGGAAACGAAAGCATCGCCCAGCCACGCAACACCCAGGATACAAATACAGGCGCTCATACCTGCTTTGAAGGTGCTGGAGTTGAG                       >  SRR4302543.2133763/1‑94 (MQ=60)
                                                                        CGATGTTGTTGGAAACGAAAGCATCGCCCAGCCACGCAACACCCAGGATACAAATACAGGCGCTCATACCTGCTTTGAAGGTGCTGGAGTTGAG                       >  SRR4302543.4561849/1‑94 (MQ=60)
                                                                        CGATGTTGTTGGAAACGAAAGCATCGCCCAGCCACGCAACACCCAGGATACAAATACAGGCGCTCATACCTGCTTTGAAGGTGCTGGAGTTGAG                       >  SRR4302543.1595640/1‑94 (MQ=60)
                                                                         GATGTTGTTGGAAACGAAAGCATCGCCCAGCCACGCAACACCCAGGATACAAATACAGGCGCTCATACCTGCTTTGAAGGTGCTGGAGTTGAGG                      <  SRR4302543.6768643/94‑1 (MQ=60)
                                                                         GATGTTGTTGGAAACGAAAGCATCGCCCAGCCACGCAACACCCAGGATACAAATACAGGCGCTCATACCTGCTCTGAAGGTGCTGGAGTTGAGG                      >  SRR4302543.596590/1‑94 (MQ=60)
                                                                          ATGTTGTTGGAAACGAAAGCATCGCCCAGCCACGCAACACCCAGGATACAAATACAGGCGCTCATACCTGCTTTGAAGGTGCTGGAGTTGAGGG                     >  SRR4302543.1263102/1‑94 (MQ=60)
                                                                          ATGTTGTTGGAAACGAAAGCATCGCCCAGCCACGCAACACCCAGGATACAAATACAGGCGCTCATACCTGCTTTGAAGGTGCTGGAGTTGAGGG                     >  SRR4302543.7946544/1‑94 (MQ=60)
                                                                          ATGTTGTTGGAAACGAAAGCATCGCCCAGCCACGCAACACCCAGGATACAAATACAGGCGCTCATACCTGCTTTGAAGGTGCTGGAGTTGAGGG                     <  SRR4302543.275121/94‑1 (MQ=60)
                                                                          ATGTTGTTGGAAACGAAAGCATCGCCCAGCCACGCAACACCCAGAATACAAATACAGGCGCTCATACCTGCTTTGAAGGTGCTGGAGTTGAGGG                     >  SRR4302543.4974588/1‑94 (MQ=60)
                                                                           TGTTGTTGGAAACGAAAGCATCGCCCAGCCACGCAACACCCAGGATACAAATACAGGCGCTCATACCTGCTTTGAAGGTGCTGGAGTTGAGGGT                    <  SRR4302543.3911824/94‑1 (MQ=60)
                                                                           TGTTGTTGGAAACGAAAGCATCGCCCAGCCACGCAACACCCAGGATACAAATACAGGCGCTCATACCTGCTTTGAAGGTGCTGGAGTTGAGGGT                    <  SRR4302543.2876575/94‑1 (MQ=60)
                                                                           TGTTGTTGGAAACGAAAGCATCGCCCAGCCACGCAACACCCAGGATACAAATACAGGCGCTCATACCTGCTTTGAAGGTGCTGGAGTTGAGGGT                    <  SRR4302543.1570483/94‑1 (MQ=60)
                                                                             TTGTTGGAAACGAAAGCATCGCCCAGCCACGCAACACCCAGGATACAAATACAGGCGCTCATACCTGCTTTGAAGGTGCTGGAGTTGAGGGTGT                  <  SRR4302543.5150473/94‑1 (MQ=60)
                                                                             TTGTTGGAAACGAAAGCATCGCCCAGCCACGCAACACCCAGGATACAAATACAGGCGCTCATACCTGCTTTGAAGGTGCTGGAGTTGAGGGTGT                  <  SRR4302543.1567127/94‑1 (MQ=60)
                                                                              TGTTGGAAACGAAAGCATCGCCCAGCCACGCAACACCCAGGATACAAATACAGGCGCTCATACCTGCTTTGAAGGTGCTGGAGTTGAGGGTGTT                 <  SRR4302543.3208543/94‑1 (MQ=60)
                                                                              TGTTGGAAACGAAAGCATCGCCCAGCCACGCAACACCCAGGATACAAATACAGGCGCTCATACCTGCTTTGAAGGTGCTGGAGTTGAGGGTGTT                 <  SRR4302543.313716/94‑1 (MQ=60)
                                                                               GTTGGAAACGAAAGCATCGCCCAGCCACGCAACACCCAGGATACAAATACAGGCGCTCATACCTTCTTTGTATGTGCTGGTGTTGAGGGTGTTG                >  SRR4302543.812563/1‑94 (MQ=60)
                                                                               GGTGGAAACGAAAGCATCGCCCAGCCACGCAACACCCAGGATACAAATACAGGCGCTCATACCTGCTTTGAAGGTGCTGGAGTTGAGGGTGTTG                <  SRR4302543.2406570/94‑1 (MQ=60)
                                                                                  GGAAACGAAAGCATCGCCCAGCCACGCAACACCCAGGATACAAATACAGGCGCTCATACCTGCTTTGAAGGTGCTGGAGTTGAGGGTGTTGTCG             <  SRR4302543.2841582/94‑1 (MQ=60)
                                                                                   GAAACGAAAGCATCGCCCAGCCACGCAACACCCAGGATACAAATACAGGCGCTCATACCTGCTTTGAAGGTGCTGGAGTTGAGGGTGTTGTCGG            <  SRR4302543.3359767/94‑1 (MQ=60)
                                                                                   GAAACGAAAGCATCGCCCAGCCACGCAACACCCAGGATACAAATACAGGCGCTCATACCTGCTTTGAAGGTGCTGGAGTTGAGGGTGTTGTCGG            <  SRR4302543.5499775/94‑1 (MQ=60)
                                                                                   GAAACGAAAGCATCGCCCAGCCACGCAACACCCAGGATACAAATACAGGCGCTCATACCTGCTTTGAAGGTGCTGGAGTTGAGGGTGTTGTCGG            >  SRR4302543.5450495/1‑94 (MQ=60)
                                                                                    AAACGAAAGCATCGCCCAGCCACGCAACACCCAGGATACAAATACAGGCGCTCATACCTGCTTTGAAGGTGCTGGAGTTGAGGGTGTTGTCGGT           <  SRR4302543.2789450/94‑1 (MQ=60)
                                                                                    AAACGAAAGCATCGCCCAGCCACGCAACACCCAGGATACAAATACAGGCGCTCATACCTGCTTTGAAGGTGCTGGAGTTGAGGGTGTTGTCGGT           <  SRR4302543.3743971/94‑1 (MQ=60)
                                                                                    AAACGAAAGCATCGCCCAGCCACGCAACACCCAGGATACAAATACAGGCGCTCATACCTGCTTTGAAGGTGCTGGAGTTGAGGGTGTTGTCGGT           >  SRR4302543.3656173/1‑94 (MQ=60)
                                                                                    AAACGAAAGCATCGCCCAGCCACGCAACACCCAGGATACAAATACAGGCGCTCATACCTGCTTTGAAGGTGCTGGAGTTGAGGGTGTTGTCGGT           >  SRR4302543.1767574/1‑94 (MQ=60)
                                                                                    AAACGAAAGCATCGCCCAGCCACGCAACACCCAGGATACAAATACAGGCGCTCATACCTGCTTTGAAGGTGCTGGAGTTGAGGGTGTTGTCGGT           <  SRR4302543.7048787/94‑1 (MQ=60)
                                                                                    AAACGAAAGCATCGCCCAGCCACGCAACACCCAGGATACAAATACAGGCGCTCATACCTGCTTTGAAGGTGCTGGAGTTGAGGGTGTTGTCGGT           >  SRR4302543.4443434/1‑94 (MQ=60)
                                                                                      ACGAAAGCATCGCCCAGCCACGCAACACCCAGGATACAAATACAGGCGCTCATACCTGCTTTGAAGGTGCTGGAGTTGAGGGTGTTGTCGGTAT         >  SRR4302543.2931418/1‑94 (MQ=60)
                                                                                      ACGAAAGCATCGCCCAGCCACGCAACACCCAGGATACAAATACAGGCGCTCATACCTGCTTTGAAGGTGCTGGAGTTGAGGGTGTTGTCGGTAT         >  SRR4302543.3403190/1‑94 (MQ=60)
                                                                                       CGAAAGCATCGCCCAGCCACGCAACACCCAGGATACAAATACAGGCGCTCATACCTGCTTTGAAGGTGCTGGAGTTGAGGGTGTTGTCGGTATC        >  SRR4302543.1874857/1‑94 (MQ=60)
                                                                                       CGAAAGCATCGCCCAGCCACGCAACACCCAGGATACAAATACAGGCGCTCATACCTGCTTTGAAGGTGCTGGAGTTGAGGGTGTTGTCGGTATC        >  SRR4302543.4824777/1‑94 (MQ=60)
                                                                                       CGAAAGCATCGCCCAGCCACGCAACACCCAGGATACAAATACAGGCGCTCATACCTGCTTTGAAGGTGCTGGAGTTGAGGGTGTTGTCGGTATC        <  SRR4302543.1481362/94‑1 (MQ=60)
                                                                                       CGAAAGCATCGCCCAGCCACGCAACACCCAGGATACAAATACAGGCGCTCATACCTGCTTTGAAGGTGCTGGAGTTGAGGGTGTTGTCGGTATC        >  SRR4302543.4436697/1‑94 (MQ=60)
                                                                                        GAAAGCATCGCCCAGCCACGCAACACCCAGGATACAAATACAGGCGCTCATTCCTGCTTTGAAGGTGCTGGAGTTGAGAGTGTTGTCGGTATCG       <  SRR4302543.4932764/94‑1 (MQ=60)
                                                                                        GAAAGCATCGCCCAGCCACGCAACACCCAGGATACAAATACAGGCGCTCATACCTGCTTTGAAGGTGCTGGAGTTGAGGGTGTTGTCGGTATCG       >  SRR4302543.7925549/1‑94 (MQ=60)
                                                                                        GAAAGCATCGCCCAGCCACGCAACACCCAGGATACAAATACAGGCGCTCATACCTGCTTTGAAGGTGCTGGAGTTGAGGGTGTTGTCGGTATCG       >  SRR4302543.2548851/1‑94 (MQ=60)
                                                                                          AAGCATCGCCCAGCCACGCAACACCCAGGATACAAATACAGGCGCTCATACCTGCTTTGAAGGTGCTGGAGTTGAGGGTGTTGTCGGTATCGAC     <  SRR4302543.3955382/94‑1 (MQ=60)
                                                                                          AAGCATCGCCCAGCCACGCAACACCCAGGATACAAATACAGGCGCTCATACCTGCTTTGAAGGTGCTGGAGTTGAGGGTGTTGTCGGTATCGAC     >  SRR4302543.1318536/1‑94 (MQ=60)
                                                                                           AGCATCGCCCAGCCACGCAACACCCAGGATACAAATACAGGCGCTCATACCTGCTTTGAAGGTGCTGGAGTTGAGGGTGTTGTCGGTATCGACT    >  SRR4302543.4666626/1‑94 (MQ=60)
                                                                                           AGCATCGCCCAGCCACGCAACACCCAGGATACAAATACAGGCGCTCATACCTGCTTTGAAGGTGCTGGAGTTGAGGGTGTTGTCGGTATCGACT    >  SRR4302543.4666625/1‑94 (MQ=60)
                                                                                           AGCATCGCCCAGCCACGCAACACCCAGGATACAAATACAGGCGCTCATACCTGCTTTGAAGGTGCTGGAGTTGAGGGTGTTGTCGGTATCGACT    >  SRR4302543.5881636/1‑94 (MQ=60)
                                                                                            GCATCGCCCAGCCACGCAACACCCAGGATACAAATACAGGCGCTCATACCTGCTTTGAAGGTGCTGGAGTTGAGGGTGTTGTCGGTATCGACTT   <  SRR4302543.1661013/94‑1 (MQ=60)
                                                                                             CATCGCCCAGCCACGCAACACCCAGGATACAAATACAGGCGCTCATACCTGCTTTGAAGGTGCTGGAGTTGAGGGTGTTGTCGGTATCGACTTT  <  SRR4302543.7979605/94‑1 (MQ=60)
                                                                                             |                                                                                             
CAAAGAAGAAGATGACGGCCAGCAGCCACGGATGACCCTGAATCACTTCACCAGCGGTATCTTTGATCCAGTCGATGTTGTTGGAAACGAAAGTATCGCCCAGCCACGCAACACCCAGGATACAAATACAGGCGCTCATACCTGCTTTGAAGGTGCTGGAGTTGAGGATGTTGTCGGTATCGACTTT  >  NC_000913/4363334‑4363520

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑