Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 1696 279.3 15874187 97.8% 15524954 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,484,261 T→C L159P (CTG→CCG)  yjgP → hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,484,2610TC99.4% 972.1 / ‑5.0 308L159P (CTG→CCG) yjgPhypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (2/0);  new base C (160/146);  total (162/146)
Fisher's exact test for biased strand distribution p-value = 5.00e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.83e-01

Only 100 of 324 total aligned reads displayed.

ATGAAGTGTTAGCAGAAGCGAAAGCGAACCCTGGCATGGCGGCGCTGGCGCAAGGGCAATTCCAGCAAGCGACTAATGGCAGCTCGGTGCTGTTCATCGAAAGCGTTGACGGCAGCGATTTCAAAGATGTGTTCCTCGCGCAAATTCGACCAAAAGGTAATGCACGTCCTTCTGTGGTGGTG  >  NC_000913/4484171‑4484352
                                                                                          |                                                                                           
   aaGTGTTAGCAGAAGCGAAAGCGAACCCTGGCATGGCGGCGCTGGCGCAAGGGTAATTCCAGCAAGCGACTAATGGCAGCTCGGTGCCGTTCa                                                                                        <  2:4368573/93‑1 (MQ=255)
    aGTGTTAGCAGAAGCGAAAGCGAACCCTGGCATGGCGGCGCTGGCGCAAGGGCAATTCCAGCAAGCGACTAATGGCAGCTCGGTGCCGTTCAt                                                                                       >  2:7708045/1‑93 (MQ=255)
        ttAGCAGAAGCGAAAGCGAACCCTGGCATGGCGGCGCTGGCGCAAGGGCAATTCCAGCAAGCGACTAATGGCAGCTCGGTGCCGTTCATCGaa                                                                                   >  2:415730/1‑93 (MQ=255)
          aGCAGAAGCGAAAGCGAACCCTGGCATGGCGGCGCTGGCGCAAGGGCAATTCCAGCAAGCGACTAATGGCAGCTCGGTGCCGTTCATCGAAAg                                                                                 <  1:940753/93‑1 (MQ=255)
          aGCAGAAGCGAAAGCGAACCCTGGCATGGCGGCGCTGGCGCAAGGGCAATTCCAGCAAGCGACTAATGGCAGCTCGGTGCCGGTCATCGAAAg                                                                                 >  1:2013118/1‑93 (MQ=255)
            cAGAAGCGAAAGCGAACCCTGGCATGGCGGCGCTGGCGCAAGGGCAATTCCAGCAAGCGACTAATGGCAGCTCGGTGCCGTTCATCGAAAGCg                                                                               <  1:2736787/93‑1 (MQ=255)
             aGAAGCGAAAGCGAACCCTGGCATGGCGGCGCTGGCGCAAGGGCAATTCCAGCAAGCGACTAATGGCAGCTCGGTGCCGTTCATCGCAAGCGt                                                                              >  2:2100676/1‑93 (MQ=255)
             aGAAGCGAAAGCGAACCCTGGCATGGCGGCGCTGGCGCAAGGGCAATTCCAGCAAGCGACTAATGGCAGCTCGGTGCCGTTCATCGAAAGCGt                                                                              >  1:4537789/1‑93 (MQ=255)
              gAAGCGAAAGCGAACCCTGGCATGGCGGCGCTGGCGCAAGGGCAATTCCAGCAAGCGACTAATGGCAGCTCGGTGCCGTTCATCGAAAGCGtt                                                                             >  2:1529179/1‑93 (MQ=255)
              gAAGCGAAAGCGAACCCTGGCATGGCGGCGCTGGCGCAAGGGCAATTCCAGCAAGCGACTAATGGCAGCTCGGTGCCGTTCATCGAAAGCGtt                                                                             >  1:5085488/1‑93 (MQ=255)
              gAAGCGAAAGCGAACCCTGGCATGGCGGCGCTGGCGCAAGGGCAATTCCAGCAAGCGACTAATGGCAGCTCGGTGCCGTTCATCGAAAGCGtt                                                                             >  2:3772065/1‑93 (MQ=255)
                agcgaaagcgaaCCCTGGCATGGCGGCGCTGGCGCAAGGGCAATTCCAGCAAGCGACTAATGGCAGCTCGGTGCCGTTCATTGAAAGCGTTGa                                                                           >  2:7111904/1‑93 (MQ=255)
                agcgaaagcgaaCCCTGGCATGGCGGCGCTGGCGCAAGGGCAATTCCAGCAAGCGACTAATGGCAGCTCGGTGCCGTTCATCGAAAGCGTTGa                                                                           <  2:6674558/93‑1 (MQ=255)
                agcgaaagcgaaCCCTGGCATGGCGGCGCTGGCGCAAGGGCAATTCCAGCAAGCGACTAATGGCAGCTCGGTGCCGTTCATCGAAAGCGTTGa                                                                           >  2:755864/1‑93 (MQ=255)
                 gcgaaagcgaaCCCTGGCATGGCGGCGCTGGCGCAAGGGCAATTCCAGCAAGCGACTAATGGCAGCTCGGTGCCGTTCATCGAAAGCGTTGAc                                                                          <  1:6516362/93‑1 (MQ=255)
                   gaaagcgaaCCCTGGCATGGCGGCGCTGGCGCAAGGGCAATTCCAGCAAGCGACTAATGGCAGCTCGGTGCCGTTCATCGAAAGCGTTGACgg                                                                        <  1:3580737/93‑1 (MQ=255)
                    aaagcgaaCCCTGGCATGGCGGCGCTGGCGCAAGGGCAATTCCAGCAAGCGACTAATGGCAGCTCGGTGCCGTTCATCGAAAGCGTTGACGGc                                                                       >  2:6347636/1‑93 (MQ=255)
                     aagcgaaCCCTGGCATGGCGGCGCTGGCGCAAGGGCAATTCCAGCAAGCGACTAATGGCAGCTCGGTGCCGTTCATCGAAAGCGTTGACGGCa                                                                      >  2:6856385/1‑93 (MQ=255)
                     aagcgaaCCCTGGCATGGCGGCGCTGGCGCAAGGGCAATTCCAGCAAGCGACTAATGGCAGCTCGGTGCCGTTCATCGAAAGCGTTGACGGCa                                                                      >  2:3405145/1‑93 (MQ=255)
                     aagcgaaCCCTGGCATGGCGGCGCTGGCGCAAGGGCAATTCCAGCAAGCGACTAATGGCAGCTCGGTGCCGTTCATCGAAAGCGTTGACGGCa                                                                      >  1:7041603/1‑93 (MQ=255)
                       gcgaaCCCTGGCATGGCGGCGCTGGCGCAAGGGCAATTCCAGCAAGCGACTAATGGCAGCTCGGTGCCGTTCATCGAAAGCGTTGACGGCAGc                                                                    <  1:1819348/93‑1 (MQ=255)
                       gcgaaCCCTGGCATGGCGGCGCTGGCGCAAGGGCAATTCCAGCAAGCGACTAATGGCAGCTCGGTGCCGTTCATCGAAAGCGTTGACGGCAGc                                                                    >  2:3235999/1‑93 (MQ=255)
                       gcgaaCCCTGGCATGGCGGCGCTGGCGCAAGGGCAATTCCAGCAAGCGACTAATGGCAGCTCCGTGACGTTCATCGAAAGCGTTGACGGAAGc                                                                    >  1:48981/1‑93 (MQ=255)
                          aaCCCTGGCATGGCGGCGCTGGCGCAAGGGCAATTCCAGCAAGCGACTAATGGCAGCTCGGTGCCGTTCATCGAAAGCGTTGACGGCAGCGAt                                                                 >  1:5838313/1‑93 (MQ=255)
                           aCCCTGGCATGGCGGCGCTGGCGCAAGGGCAATTCCAGCAAGCGACTAATGGCAGCTCGGTGCCGTTCATCGAAAGCGTTGACGGCAGCGAtt                                                                >  1:4411920/1‑93 (MQ=255)
                           aCCCTGGCATGGCGGCGCTGGCGCAAGGGCAATTCCAGCAAGCGACTAATGGCAGCTCGGTGCCGTTCATCGAAAGCGTTGACGGCAGCGAtt                                                                >  1:7373721/1‑93 (MQ=255)
                           aCCCTGGCATGGCGGCGCTGGCGCAAGGGCAATTCCAGCAAGCGACTAATGGCAGCTCGGTGCCGTTCATCGAAAGCGTTGACGGCAGCGAtt                                                                <  2:2239983/93‑1 (MQ=255)
                             ccTGGCATGGCGGCGCTGGCGCAAGGGCAATTCCAGCAAGCGACTAATGGCAGCTCGGTGCCGTTCATCGAAAGCGTTGACGGCAGCGATTTc                                                              <  2:1948067/93‑1 (MQ=255)
                              cTGGCATGGCGGCGCTGGCGCAAGGGCAATTCCAGCAAGCGACTAATGGCAGCTCGGTGCCGTTCATCGAAAGCGTTGACGGCAGCGATTTCa                                                             >  2:5208560/1‑93 (MQ=255)
                              cTGGCATGGCGGCGCTGGCGCAAGGGCAATTCCAGCAAGCGACTAATGGCAGCTCGGTGCCGTTCATCGAAAGCGTTGACGGCAGCGATTTCa                                                             >  1:7930465/1‑93 (MQ=255)
                               tGGCATGGCGGCGCTGGCGCAAGGGCAATTCCAGCAAGCGACTAATGGCAGCTCGGTGCCGTTCATCGAAAGCGTTGACGGCAGCGATTTCaa                                                            >  2:3634718/1‑93 (MQ=255)
                                ggCATGGCGGCGCTGGCGCAAGGGCAATTCCAGCAAGCGACTAATGGCAGCTCGGTGCCGTTCATCGAAAGCGTTGACGGCAGCGATTTCaaa                                                           <  1:3266845/93‑1 (MQ=255)
                                 gCATGGCGGCGCTGGCGCAAGGGCAATTCCAGCAAGCGACTAATGGCAGCTTGGTGCCGTTCATCGAAATCGTTGACGGCAGCGATTTCAAAg                                                          <  2:6955323/93‑1 (MQ=255)
                                 gCATGGCGGCGCTGGCGCAAGGGCAATTCCAGCAAGCGACTAATGGCAGCTCGGTGCCGTTCATCGAAAGCGTTGACGGCAGCGATTTCAAAg                                                          <  1:1559927/93‑1 (MQ=255)
                                 gCATGGCGGCGCTGGCGCAAGGGCAATTCCAGCAAGCGACTAATGGCAGCTCGGTGCCGTTCATCGAAAGCGTTGACGGCAGCGATTTCAAAg                                                          <  1:6779779/93‑1 (MQ=255)
                                  cATGGCGGCGCTGGCGCAAGGGCAATTCCAGCAAGCGACTAATGGCAGCTCGGTGCCGTTCATCGAAAGCGTTGACGGCAGCGATTTCAAAGa                                                         <  2:533436/93‑1 (MQ=255)
                                  cATGGCGGCGCTGGCGCAAGGGCAATTCCAGCAAGCGACTAATGGCAGCTCGGTGCCGTTCATCGAAAGCGTTGACGGCAGCGATTTCAAAGa                                                         >  1:639986/1‑93 (MQ=255)
                                    tGGCGGCGCTGGCGCAAGGGCAATTCCAGCAAGCGACTAATGGCAGCTCGGTGCCGTTCATCGAAAGCGTTGACGGCAGCGATTTCAAAGAtg                                                       <  2:2651153/93‑1 (MQ=255)
                                    tGGCGGCGCTGGCGCAAGGGCAATTCCAGCAAGCGACTAATGGCAGCTCGGTGCCGTTCATCGAAAGCGTTGACGGCAGCGATTTCAAAGAtg                                                       <  2:6143872/93‑1 (MQ=255)
                                    tGGCGGCGCTGGCGCAAGGGCAATTCCAGCAAGCGACTAATGGCAGCTCGGTGCCGTTCATCGAAAGCGTTGACGGCAGCGATTTCAAAGAtg                                                       <  2:3005656/93‑1 (MQ=255)
                                    tGGCGGCGCTGGCGCAAGGGCAATTACAGCAAGCGACTAATGGCAGCTCGGTGAAGTTCATCGAAAGCGTTGACGGCAGCGATTTCAAAGAtg                                                       <  1:4852948/93‑1 (MQ=255)
                                     ggcggcGCTGGCGCAAGGGCAATTCCAGCAAGCGACTAATGGCAGCTCGGTGCCGTTCATCGAAAGCGTTGACGGCAGCGATTTCAAAGAtgt                                                      <  1:7229978/93‑1 (MQ=255)
                                      gcggcgCTGGCGCAAGGGCAATTCCAGCAAGCGACTAATGGCAGCTCGGTGCCGTTCATCGAAAGCGTTGACGGCAGCGATTTCAAAGAtgtg                                                     >  1:5086057/1‑93 (MQ=255)
                                       gggcgCTGGCGCAAGGGCAATTCCAGCAAGCGACTAATGGCAGCTCGGTGCCGTTCATCGAAAGCGTTGACGGCAGCGATTTCAAAGATgtgt                                                    <  2:1488612/92‑1 (MQ=255)
                                        ggcgCTGGCGCAAGGGCAATTCCAGCAAGCGACTAATGGCAGCTCGGTGCCGTTCATCGAAAGCGTTGACGGCAGCGATTTCAAAGATGTGtt                                                   >  1:6719717/1‑93 (MQ=255)
                                        ggcgCTGGCGCAAGGGCAATTCCAGCAAGCGACTAATGGCAGCTCGGTGCCGTTCATCGAAAGCGTTGACGGCAGCGATTTCAAAGATGTGtt                                                   >  2:6422031/1‑93 (MQ=255)
                                         gcgcTGGCGCAAGGGCAATTCCAGCAAGCGACTAATGGCAGCTCGGTGCCGTTCATCGAAAGCGTTGACGGCAGCGATTTCAAAGATGTGTTc                                                  <  1:2494127/93‑1 (MQ=255)
                                         gcgcTGGCGCAAGGGCAATTCCAGCAAGCGACTAATGGCAGCTCGGTGCCGTTCATCGAAAGCGTTGACGGCAGCGATTTCAAAGATGTGTTc                                                  >  2:1225642/1‑93 (MQ=255)
                                           gcTGGCGCAAGGGCAATTCCAGCAAGCGACTAATGGCAGCTCGGTGCCGTTCATCGAAAGCGTTGACGGCAGCGATTTCAAAGATGTGTTCCt                                                >  1:5659804/1‑93 (MQ=255)
                                            cTGGCGCAAGGGCAATTCCAGCAAGCGACTAATGGCAGCTCGGTGCCGTTCATCGAAAGCGTTGACGGCAGCGATTTCAAAGATGTGTTCCTc                                               <  2:2958002/93‑1 (MQ=255)
                                              ggCGCAAGGGCAATTCCAGCAAGCGACTAATGGCAGCTCGGTGCCGTTCATCGAAAGCGTTGACGGCAGCGATTTCAAAGATGTGTTCCTcgc                                             >  2:4823921/1‑93 (MQ=255)
                                                   aaGGGCAATTCCAGCAAGCGACTAATGGCAGCTCGGTGCCGTTCATCGCAAGCGTTGACGGCAGCGATTTCAAAGATGTGTTCCTCGCGCaaa                                        >  2:2386131/1‑93 (MQ=255)
                                                   aaGGGCAATTCCAGCAAGCGACTAATGGCAGCTCGGTGCCGTTCATCGAAAGCGTTGACGGCAGCGCTTTCAAAGATGTGTTCCTCGCGCaaa                                        >  1:7600863/1‑93 (MQ=255)
                                                    aGGGCAATTCCAGCAAGCGACTAATGGCAGCTCGGTGCCGTTCATCGAAAGCGTTGACGGCAGCGATTTCAAAGATGTGTTCCTCGCGCAAAt                                       <  1:801285/93‑1 (MQ=255)
                                                    aGGGCAATTCCAGCAAGCGACTAATGGCAGCTCGGTGCCGTTCATCGAAAGCGTTGACGGCAGCGATTTCAAAGATGTGTTCCTCGCGCAAAt                                       >  2:7854364/1‑93 (MQ=255)
                                                     gggCAATTCCAGCAAGCGACTAATGGCAGCTCGGTGCCGTTCATCGAAAGCGTTGACGGCAGCGATTTCAAAGATGTGTTCCTCGCGCAAAtt                                      <  1:5774410/93‑1 (MQ=255)
                                                     gggCAATTCCAGCAAGCGACTAATGGCAGCTCGGTGCCGTTCATCGAAAGCGTTGACGGCAGCGATTTCAAAGATGTGTTCCTCGCGCAAAtt                                      <  2:3050598/93‑1 (MQ=255)
                                                     gggCAATTCCAGCAAGCGACTAATGGCAGCTCGGTGCCGTTCATCGAAAGCGTTGACGGCAGCGATTTCAAAGATGTGTTCCTCGCGCAAAtt                                      >  1:5988517/1‑93 (MQ=255)
                                                     gggCAATTCCAGCAAGCGACTAATGGCAGCTCGGTGCCGTTCATCGAAAGCGTTGACGGCAGCGATTTCAAAGATGTGTTCCTCGCGCAAAtt                                      <  1:5548224/93‑1 (MQ=255)
                                                     gggCAATTCCAGCAAGCGACTAATGGCAGCTCGGTGCCGTTCATCGAAAGCGTTGACGGCAGCGATTTCAAAGATGTGTTCCTCGCGCAAAtt                                      <  2:5918202/93‑1 (MQ=255)
                                                     gggCAATTCCAGCAAGCGACTAATGGCAGCTCGGTGCCGTTCATCGAAAGCGTTGACGGCAGCGATTTCAAAGATGTGTTCCTCGCGCAAAtt                                      <  1:1062956/93‑1 (MQ=255)
                                                      ggCAATTCCAGCAAGCGACTAATGGCAGCTCGGTGCCGTTCATCGAAAGCGTTGACGGCAGCGATTTCAAAGATGTGTTCCTCGCGCAAATTc                                     >  2:1021170/1‑93 (MQ=255)
                                                             ccAGCAAGCGACTAATGGCAGCTCGGTGCCGTTCATCGAAAGCGTTGACGGCAGCGATTTCAAAGATGTGTTCCTCGCGCAAATTCGACCaaa                              >  2:3991329/1‑93 (MQ=255)
                                                             ccAGCAAGCGACTAATGGCAGCTCGGTGCCGTTCATCGAAAGCGTTGACGGCAGCGATTTCAAAGATGTGTTCCTCGCGCAAATTCGACCaaa                              >  1:1209820/1‑93 (MQ=255)
                                                             ccAGCAAGCGACTAATGGCAGCTCGGTGCCGTTCATCGAAAGCGTTGACGGCAGCGATTTCAAAGATGTGTTCCTCGCGCAAATTCGACCaaa                              <  1:7727092/93‑1 (MQ=255)
                                                             ccAGCAAGCGACTAATGGCAGCTCGGTGCCGTTCATCGAAAGCGTTGACGGCAGCGATTTCAAAGATGTGTTCCTCGCGCAAATTCGACCaaa                              >  1:7397813/1‑93 (MQ=255)
                                                              cAGCAAGCGACTAATGGCAGCTCGGTGCCGTTCATCGAAAGCGTTGACGGCAGCGATTTCAAAGATGTGTTCCTCGCGCAAATTCGACCaaaa                             <  1:4097916/93‑1 (MQ=255)
                                                               aGCAAGCGACTAATGGCAGCTCGGTGCCGTTCATCGAAAGCGTTGACGGCAGCGATTTCAAAGATGTGTTCCTCGCGCAAATTCGACCAAAAg                            <  1:6652184/93‑1 (MQ=255)
                                                               aGCAAGCGACTAATGGCAGCTCGGTGCCGTTCATCGAAAGCGTTGACGGCAGCGATTTCAAAGATGTGTTCCTCGCGCAAATTCGACCAAAAg                            <  1:5241465/93‑1 (MQ=255)
                                                                gCAAGCGACTAATGGCAGCTCGGTGCCGTTCATCGAAAGCGTTGACGGCAGCGATTTCAAAGATGTGTTCCTCGCGCAAATTCGACCAAAAgg                           <  2:5042237/93‑1 (MQ=255)
                                                                 cAAGCGACTAATGGCAGCTCGGTGCCGTTCATCGAAAGCGTTGACGGCAGCGATTTCAAAGATGTGTTCCTCGCGCAAATTCGACCAAAAGGt                          >  1:34929/1‑93 (MQ=255)
                                                                 cAAGCGACTAATGGCAGCTCGGTGCCGTTCATCGAAAGCGTTGACGGCAGCGATTTCAAAGATGTGTTCCTCGCGCAAATTCGACCAAAAGGt                          <  1:2268746/93‑1 (MQ=255)
                                                                  aaGCGACTAATGGCAGCTCGGTGCCGTTCATCGAAAGCGTTGACGGCAGCGATTTCAAAGATGTGTTCCTCGCGCAAATTCGACCAAAAGGTa                         <  2:7317417/93‑1 (MQ=255)
                                                                   aGCGACTAATGGCAGCTCGTTGCCGTTCATCGAAAGCGTTGACGGCAGCGATTTCAAAGATGTGTTCCTCGCGCAAATTCGACCAAAAGGTaa                        <  1:6578411/93‑1 (MQ=255)
                                                                   aGCGACTAATGGCAGCTCGGTGCCGTTCATCGAAAGCGTTGACGGCAGCGATTTCAAAGATGTGTTCCTCGCGCAAATTCGACCAAAAGGTaa                        <  1:4719251/93‑1 (MQ=255)
                                                                     cGACTAATGGCAGCTCGGTGCCGTTCATCGAAAGCGTTGACGGCAGCGATTTCAAAGATGTGTTCCTCGCGCAAATTCGACCAAAAGGTAATg                      <  1:1352166/93‑1 (MQ=255)
                                                                      gACTAATGGCAGCTCGGTGCCGTTCATCGAAAGCGTTGACGGCAGCGCGTTCAAAGATGTGGTCCTCGCGCAAATTCGACCAAAAGGGAATGc                     >  2:4576551/1‑93 (MQ=255)
                                                                      gACTAATGGCAGCTCGGTGCCGTTCATCGAAAGCGTTGACGGCAGCGATTTCAAAGATGTGTTCCTCGCGCAAATTCGACCAAAAGGTAATGc                     >  2:7895935/1‑93 (MQ=255)
                                                                      gACTAATGGCAGCTCGGTGCCGTTCATCGAAAGCGTTGACGGCAGCGATTTCAAAGATGTGTTCCTCGCGCAAATTCGACCAAAAGGTAATGc                     <  2:5735438/93‑1 (MQ=255)
                                                                      gACTAATGGCAGCTCGGTGCCGTTCATCGAAAGCGTTGACGGCAGCGATTTCAAAGATGTGTTCCTCGCGCAAATTCGACCAAAAGGTAATGc                     <  2:6560919/93‑1 (MQ=255)
                                                                        cTAATGGCAGCTCGGTGCCGTTCATCGAAAGCGTTGACGGCAGCGATTTCAAAGATGTGTTCCTCGCGCAAATTCGACCAAAAGGTAATGCAc                   <  1:40146/93‑1 (MQ=255)
                                                                        cTAATGGCAGCTCGGTGCCGTTCATCGAAAGCGTTGACGGCAGCGATTTCAAAGATGTGTTCCTCGCGCAAATTCGACCAAAAGGTAATGCAc                   >  2:1682790/1‑93 (MQ=255)
                                                                         tAATGGCAGCTCGGTGCTGCTCATCGAAAGGGTTGACGGCAGCGATTTCAAGGATGTGTTCCGCGCGCACATCGCTCCAAAGGGAAATGcgct                  >  1:2691108/1‑90 (MQ=255)
                                                                         tAATGGCAGCTAGGTGCCGTTCATCGAAAGCGTTGACGGCAGCGATTTCAAAGATGTGTTCCTCGCGCCAATTCGACCAAAAGGTAATGCACg                  >  2:839765/1‑93 (MQ=255)
                                                                           aTGGCAGCTCGGTGCCGTTCATCGAAAGCGTTGACGGCAGCGATTTCAAAGATGTGTTCCTCGCGCAAATTCGACCAAAAGGTAATGCACGTc                <  1:2532698/93‑1 (MQ=255)
                                                                           aTGGCAGCTCGGTGCCGTTCATCGAAAGCGTTGACGGCAGCGATTTCAAAGATGTGTTCCTCGCGCAAATTCGACCAAAAGGTAATGCACGTc                >  2:5404638/1‑93 (MQ=255)
                                                                           aTGGCAGCTCGGTGCCGTTCATCGAAAGCGTTGACGGCAGCGATTTCAAAGATGTGTTCCTCGCGCAAATTCGACCAAAAGGTAATGCACGTc                <  2:3872461/93‑1 (MQ=255)
                                                                            tGGCAGCTCGGTGCCGTTCATCGAAAGCGTTGACGGCAGCGATTTCAAAGATGTGTTCCTCGCGCAAATTCGACCAAAAGGTAATGCACGTcc               >  1:3367798/1‑93 (MQ=255)
                                                                             ggCAGCTCGGTGCCGTTCATCGAAAGCGTTGACGGCAGCGATTTCAAAGATGTGTTCCTCGCGCAAATTCGACCAAAAGGTAATGCACGTCCt              <  1:78346/93‑1 (MQ=255)
                                                                               cAGCTCGGTGCCGTTCATCGAAAGCGTTGACGGCAGCGATTTCAAAGATGTGTTCCTCGCGCAAATTCGACCAAAAGGTAATGCACGTCCTTc            <  1:292126/93‑1 (MQ=255)
                                                                                 gCTCGGTGCCGTTCATCGAAAGCGTTGACGGCAGCGATTTCAAAGATGTGTTCCTCGCGCAAATTCGACCAAAAGGTAATGCACGTCCTTCtg          >  1:2364994/1‑93 (MQ=255)
                                                                                  cTCGGTGCCGTTCATCGAAAGCGTTGACGGCAGCGATTTCAAAGATGTGTTCCTCGCGCAAATTCGACCAAAAGGTAATGCACGTCCTTCtgt         <  2:4187809/93‑1 (MQ=255)
                                                                                   tCGGTGCCGTTCATCGAAAGCGTTGACGGCAGCGATTTCAAAGATGTGTTCCTCGCGCAAATTCGCCCAAACGGTAATGCACGTCCTTCtgtg        >  1:2126346/1‑93 (MQ=255)
                                                                                   tCGGTGCCGTTCATCGAAAGCGTTGACGGCAGCGATTTCAAAGATGTGTTCCTCGCGCAAATTCGACCAAAAGGTAATGCACGTCCTTCtgtg        >  1:355318/1‑93 (MQ=255)
                                                                                    cGGTGCCGTTCATCGAAAGCGTTGACGGCAGCGATTTCAAAGATGTGTTCCTCGCGCAAATTCGACCAAAAGGTAATGCACGTCCTTCTgtgg       >  2:4755189/1‑93 (MQ=255)
                                                                                     ggTGCCGTTCATCGAAAGCGTTGACGGCAGCGATTTCAAAGATGTGTTCCTCGCGCAAATTCGACCAAAAGGTAATGCACGTCCTTCTgtggt      >  2:6735678/1‑93 (MQ=255)
                                                                                      gTGCCGTTCATCGAAAGCGTTGACGGCAGCGATTTCAAAGATGTGTTCCTCGCGCAAATTCGACCAAAAGGTAATGCACGTCCTTCTgtggtg     >  1:5777353/1‑93 (MQ=255)
                                                                                      gTGCCGTTCATCGAAAGCGTTGACGGCAGCGATTTCAAAGATGTGTTCCTCGCGCAAATTCGACCAAAAGGTAATGCACGTCCTTCTgtggtg     >  1:7479010/1‑93 (MQ=255)
                                                                                       tGCCGTTCATCGAAAGCGTTGACGGCAGCGATTTCAAAGATGTGTTCCTCGCGCAAATTCGACCAAAAGGTAATGCACGTCCTTCTgtggtgg    >  1:3150962/1‑93 (MQ=255)
                                                                                         cTGTTCATCGAAAGCGTGGTCGGCGGCGAATCCAAAGATGTGTTCCGGGCGCAAATTCGACCAAAATGTAAGGCGCGTCGTTCTgtggtggtg  <  2:5017812/93‑1 (MQ=255)
                                                                                          |                                                                                           
ATGAAGTGTTAGCAGAAGCGAAAGCGAACCCTGGCATGGCGGCGCTGGCGCAAGGGCAATTCCAGCAAGCGACTAATGGCAGCTCGGTGCTGTTCATCGAAAGCGTTGACGGCAGCGATTTCAAAGATGTGTTCCTCGCGCAAATTCGACCAAAAGGTAATGCACGTCCTTCTGTGGTGGTG  >  NC_000913/4484171‑4484352

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
AGGATGAAGTGTTAGCAGAAGCGAAAGCGAACCCTGGCATGGCGGCGCTGGCGCAAGGGCAATTCCAGCAAGCGACTAATGGCAGCTCGGTGCTGTTCATCGAAAGCGTTGACGGCAGCGATTTCAAAGATGTGTTCCTCGCGCAAATTCGACCAAAAGGTAATGCACGTCCTTCTGTGGTGGTGG  >  NC_000913/4484168‑4484353
                                                                                             |                                                                                            
AGGATGAAGTGTTAGCAGAAGCGAAAGCGAACCCTGGCATGGCGGCGCTGGCGCAAGGGCAATTCCAGCAAGCGACTAATGGCAGCTCGGTGCC                                                                                              >  SRR4302543.1320350/1‑94 (MQ=60)
AGGATGAAGTGTTAGCAGAAGCGAAAGCGAACCCTGGCATGGCGGCGCTGGCGCAAGGGCAATTCCAGCAAGCGACTAATGGCAGCTCGGTGCC                                                                                              <  SRR4302543.6579766/94‑1 (MQ=60)
   ATGAAGTGTTAGCAGAAGCGAAAGCGAACCCTGGCATGGCGGCGCTGGCGCAAGGGCAATTCCAGCAAGCGACTAATGGCAGCTCGGTGCCGTT                                                                                           >  SRR4302543.2067383/1‑94 (MQ=60)
    TGAAGTGTTAGCAGAAGCGAGAGCGACCCCTGGCATGGCGGCGCTGGCGCAAGGGCAATTCCAGCAAGCGACTAATGGCAGCTCGGTGCCGTTC                                                                                          <  SRR4302543.7074193/94‑1 (MQ=60)
     GAAGTGTTAGCAGAAGCGAAAGCGAACCCTGGCATGGCGGCGCTGGCGCAAGGGCAATTCCAGCAAGCGACTAATGGCAGCTCGGTGCCGTTCA                                                                                         <  SRR4302543.2202687/94‑1 (MQ=60)
       AGTGTTAGCAGAAGCGAAAGCGAACCCTGGCATGGCGGCGCTGGCGCAAGGGCAATTCCAGCAAGCGACTAATGGCAGCTCGGTGCCGTTCATC                                                                                       >  SRR4302543.7746270/1‑94 (MQ=60)
       AGTGTTAGCAGAAGCGAAAGCGAACCCTGGCATGGCGGCGCTGGCGCAAGGGCAATTCCAGCAAGCGACTAATGGCAGCTCGGTGCCGTTCATC                                                                                       >  SRR4302543.788272/1‑94 (MQ=60)
         TGTTAGCAGAAGCGAAAGCGAACCCTGGCATGGCGGCGCTGGCGCAAGGGCAATTCCAGCAAGCGACTAATGGCAGCTCGGTGCCGTTCATCGA                                                                                     <  SRR4302543.530599/94‑1 (MQ=60)
          GTTAGCAGAAGCGAAAGCGAACCCTGGCATGGCGGCGCTGGCGCAAGGGCAATTCCAGCAAGCGACTAATGGCAGCTCGGTGCCGTTCATCGAA                                                                                    >  SRR4302543.1289329/1‑94 (MQ=60)
           TTAGCAGAAGCGAAAGCGAACCCTGGCATGGCGGCGCTGGCGCAAGGGCAATTCCAGCAAGCGACTAATGGCAGCTCGGTGCCGTTCATCGAAA                                                                                   >  SRR4302543.2633639/1‑94 (MQ=60)
            TAGCAGAAGCGAAAGCGAACCCTGGCATGGCGGCGCTGGCGCAAGGGCAATTCCAGCAAGCGACTAATGGCAGCTCGGTGCCGTTCATCGAAAG                                                                                  >  SRR4302543.7823717/1‑94 (MQ=60)
            TAGCAGAAGCGAAAGCGAACCCTGGCATGGCGGCGCTGGCGCAAGGGCAATTCCAGCAAGCGACTAATGGCAGCTCGGTGCCGGTCATCGAAAG                                                                                  >  SRR4302543.2031211/1‑94 (MQ=60)
             AGCAGAAGCGAAAGCGAACCCTGGCATGGCGGCGCTGGCGCAAGGGCAATTCCAGCAAGCGACTAATGGCAGCTCGGTGCCGTTCATCGAAAGC                                                                                 <  SRR4302543.948973/94‑1 (MQ=60)
             AGCAGAAGCGAAAGCGAACCCTGGCATGGCGGCGCTGGCGCAAGGGCAATTCCAGCAAGCGACTAATGGCAGCTCGGTGCCGTTCATCGAAAGC                                                                                 >  SRR4302543.2314819/1‑94 (MQ=60)
               CAGAAGCGAAAGCGAACCCTGGCATGGCGGCGCTGGCGCAAGGGCAATTCCAGCAAGCGACTAATGGCAGCTCGGTGCCGTTCATCGAAAGCGT                                                                               <  SRR4302543.2761687/94‑1 (MQ=60)
               CAGAAGCGAAAGCGAACCCTGGCATGGCGGCGCTGGCGCAAGGGCAATTCCAGCAAGCGACTAATGGCAGCTCGGTGCCGTTCATCGAAAGCGT                                                                               >  SRR4302543.7494929/1‑94 (MQ=60)
               CAGAAGCGAAAGCGAACCCTGGCATGGCGGCGCTGGCGCAAGGGCAATTCCAGCAAGCGACTAATGGCAGCTCGGTGCCGTTCATCGAAAGCGT                                                                               >  SRR4302543.4579101/1‑94 (MQ=60)
                AGAAGCGAAAGCGAACCCTGGCATGGCGGCGCTGGCGCAAGGGCAATTCCAGCAAGCGACTAATGGCAGCTCGGTGCCGTTCATCGAAAGCGTT                                                                              >  SRR4302543.5131592/1‑94 (MQ=60)
                 GAAGCGAAAGCGAACCCTGGCATGGCGGCGCTGGCGAAAGGGCAATTCCAGCAAGGGACTAATGGCAGCTCGGTGCCGTTCATCGAAAGCGTTG                                                                             <  SRR4302543.7379272/94‑1 (MQ=60)
                   AGCGAAAGCGAACCCTGGCATGGCGGCGCTGGCGCAAGGGCAATTCCAGCAAGCGACTAATGGCAGCTCGGTGCCGTTCATCGAAAGCGTTGAC                                                                           <  SRR4302543.5182750/94‑1 (MQ=60)
                   AGCGAAAGCGAACCCTGGCATGGCGGCGCTGGCGCAAGGGCAATTCCAGCAAGCGACTAATGGCAGCTCGGTGCCGTTCATCGAAAGCGTTGAC                                                                           >  SRR4302543.590337/1‑94 (MQ=60)
                    GCGAAAGCGAACCCTGGCATGGCGGCGCTGGCGCAAGGGCAATTCCAGCAAGCGACTAATGGCAGCTCGGTGCCGTTCATCGAAAGCGTTGACG                                                                          <  SRR4302543.6577732/94‑1 (MQ=60)
                    GCGAAAGCGAACCCTGGCATGGCGGCGCTGGCGCAAGGGCAATTCCAGCAAGCGACTAATGGCAGCTCGGTGCCGTTCATCGAAAGCGTTGACG                                                                          <  SRR4302543.6577733/94‑1 (MQ=60)
                    GCGAAAGCGAACCCTGGCATGGCGGCGCTGGCGCAAGGGCAATTCCAGCAAGCGACTAATGGCAGCTCGGTGCCGTTCATCGAAAGCGTTGACG                                                                          <  SRR4302543.4124741/94‑1 (MQ=60)
                     CGAAAGCGAACCCTGGCATGGCGGCGCTGGCGCAAGGGCAATTCCAGCAAGCGACTAATGGCAGCTCGGTGCCGTTCATCGAAAGCGTTGACGG                                                                         <  SRR4302543.2065497/94‑1 (MQ=60)
                     CGAAAGCGAACCCTGGCATGGCGGCGCTGGCGCAAGGGCAATTCCAGCAAGCGACTAATGGCAGCTCGGTGCCGTTCATCGAAAGCGTTGACGG                                                                         >  SRR4302543.4058798/1‑94 (MQ=60)
                      GAAAGCGAACCCTGGCATGGCGGCGCTGGCGCAAGGGCAATTCCAGCAAGCGACTAATGGCAGCTCGGTGCCGTTCCTCGAAAGCGTTGACGGC                                                                        >  SRR4302543.7594661/1‑94 (MQ=60)
                      GAAAGCGAACCCTGGCATGGCGGCGCTGGCGCAAGGGCAATTCCAGCAAGCGACTAATGGCAGCTCGGTGCCGTTCATCGAAAGCGTTGACGGC                                                                        <  SRR4302543.3612372/94‑1 (MQ=60)
                       AAAGCGAACCCTGGCATGGCGGCGCTGGCGCAAGGGCAATTCCAGCAAGCGACTAATGGCAGCTCGGTGCCGTTCATCGAAAGCGTTGACGGCA                                                                       >  SRR4302543.7109624/1‑94 (MQ=60)
                        AAGCGAACCCTGGCATGGCGGCGCTGGCGCAAGGGCAATTCCAGCAAGCGACTAATGGCAGCTCGGTGCCGTTCATCGAAAGCGTTGACGGCAG                                                                      >  SRR4302543.6137045/1‑94 (MQ=60)
                         AGCGAACCCTGGCATGGCGGCGCTGGCGCAAGGGCAATTCCAGCAAGCGACTAATGGCAGCTCGGTGCCGTTCATCGAAAGCGTTGACGGCAGC                                                                     >  SRR4302543.2843414/1‑94 (MQ=60)
                         AGCGAACCCTGGCATGGCGGCGCTGGCGCAAGGGCAATTCCAGCAAGCGACTAATGGCAGCTCGGTGCCGTTCATCGAAAGCGTTGACGGCAGC                                                                     <  SRR4302543.7453828/94‑1 (MQ=60)
                         AGCGAACCCTGGCATGGCGGCGCTGGCGCAAGGGCAATTCCAGCAAGCGACTAATGGCAGCTCGGTGCCGTTCATCGAAAGCGTTGACGGCAGC                                                                     <  SRR4302543.7942752/94‑1 (MQ=60)
                         AGCGAACCCTGGCATGGCGGCGCTGGCGCAAGGGCAATTCCAGCAAGCGACTAATGGCAGCTCGGTGCCGTTCATCGAAAGCGTTGACGGCAGC                                                                     <  SRR4302543.2219678/94‑1 (MQ=60)
                         AGCGAACCCTGGCATGGCGGCGCTGGCGCAAGGGCAATTCCAGCAAGCGACTAATGGCAGCTCGGTGCCGTTCATCGAAAGCGTCGACGGCAGC                                                                     <  SRR4302543.7287184/94‑1 (MQ=60)
                         AGCGAACCCTGGCATGGCGGCGCTGGCGCAAGGGCAATTCCAGCAAGCGACTAATGGCAGCTCCGTGACGTTCATCGAAAGCGTTGACGGAAGC                                                                     >  SRR4302543.49425/1‑94 (MQ=60)
                          GCGAACCCTGGCATGGCGGCGCTGGCGCAAGGGCAATTCCAGCAAGCGACTAATGGCAGCTCGGTGCCGTTCATCGAAAGCGTTGACGGCAGCG                                                                    <  SRR4302543.1835675/94‑1 (MQ=60)
                            GAACCCTGGCATGGCGGCGCTGGCGCAAGGGCAATTCCAGCAAGCGACTAATGGCAGCTCGGTGCCGTTCATCGAAAGCGTTGACGGCAGCGAT                                                                  >  SRR4302543.2540026/1‑94 (MQ=60)
                            GAACCCTGGCATGGCGGCGCTGGCGCAAGGGCAATTCCAGCAAGCGACTAATGGCAGCTCGGTGCCGTTCATCGAAAGCGTTGACGGCAGCGAT                                                                  >  SRR4302543.5891417/1‑94 (MQ=60)
                            GAACCCTGGCATGGCGGCGCTGGCGCAAGGGCAATTCCAGCAAGCGACTAATGGCAGCTCGGTGCCGTTCATCGAAAGCGTTGACGGCAGCGAT                                                                  >  SRR4302543.6665771/1‑94 (MQ=60)
                            GAACCCTGGCATGGCGGCGCTGGCGCAAGGGCAATTCCAGCAAGCGACTAATGGCAGCTCGGTGCCGTTCATCGAAAGCGTTGACGGCAGCGAT                                                                  >  SRR4302543.6487812/1‑94 (MQ=60)
                             AACCCTGGCATGGCGGCGCTGGCGCAAGGGCAATTCCAGCAAGCGACTAATGGCAGCTCGGTGCCGTTCATCGAAAGCGTTGACGGCAGCGATT                                                                 >  SRR4302543.7444606/1‑94 (MQ=60)
                             AACCCTGGCATGGCGGCGCTGGCGCAAGGGCAATTCCAGCAAGCGACTAATGGCAGCTCGGTGCCGTTCATCGAAAGCGTTGACGGCAGCGATT                                                                 >  SRR4302543.4451909/1‑94 (MQ=60)
                              ACCCTGGCATGGCGGCGCTGGCGCAAGGGCAATTCCAGCAAGCGACTAATGGCAGCTCGGTGCCGTTCATCGAAAGCGTTGACGGCAGCGATTT                                                                >  SRR4302543.6729192/1‑94 (MQ=60)
                               CCCTGGCATGGCGGCGCTGGCGCAAGGGCAATTCCAGCAAGCGACTAATGGCAGCTCGGTGCCGTTCATCGAAAGCGTTGACGGCAGCGATTTC                                                               <  SRR4302543.5299962/94‑1 (MQ=60)
                                CCTGGCATGGCGGCGCTGGCGCAAGGGCAATTCCAGCAAGCGACTAATGGCAGCTCGGTGCCGTTCATCGAAAGCGTTGACGGCAGCGATTTCA                                                              >  SRR4302543.1815201/1‑94 (MQ=60)
                                CCTGGCATGGCGGCGCTGGCGCAAGGGCAATTCCAGCAAGCGACTAATGGCAGCTCGGTGCCGTTCATCGAAAGCGTTGACGGCAGCGATTTCA                                                              >  SRR4302543.8004918/1‑94 (MQ=60)
                                 CTGGCATGGCGGCGCTGGCGCAAGGGCAATTCCAGCAAGCGACTAATGGCAGCTCGGTGCCGTTCATCGAAAGCGTTGACGGCAGCGATTTCCA                                                             >  SRR4302543.943706/1‑94 (MQ=60)
                                  TGGCATGGCGGCGCTGGCGCAAGGGCAATTCCAGCAAGCGACTAATGGCAGCTCGGTGCCGTTCATCGAAAGCGTTGACGGCAGCGATTTCAAA                                                            >  SRR4302543.1894516/1‑94 (MQ=60)
                                   GGCATGGCGGCGCTGGCGCAAGGGCAATTCCAGCAAGCGACTAATGGCAGCTCGGTGCCGTTCATCGAAAGCGTTGACGGCAGCGATTTCAAAG                                                           <  SRR4302543.5829682/94‑1 (MQ=60)
                                   GGCATGGCGGCGCTGGCGCAAGGGCAATTCCAGCAAGCGACTAATGGCAGCTCGGTGCCGTTCATCGAAAGCGTTGACGGCAGCGATTTCAAAG                                                           <  SRR4302543.6199741/94‑1 (MQ=60)
                                   GGCATGGCGGCGCTGGCGCAAGGGCAATTCCAGCAAGCGACTAATGGCAGCTCGGTGCCGTTCATCGAAAGCGTTGACGGCAGCGATTTCAAAG                                                           <  SRR4302543.3296364/94‑1 (MQ=60)
                                   GGCATGGCGGCGCTGGCGCAAGGGCAATTCCAGCAAGCGACTAATGGCAGCTCGGTGCCGTTCATCGAAAGCCTTGACGGCAGCGATTTCAAAG                                                           >  SRR4302543.357290/1‑94 (MQ=60)
                                    GCATGGCGGCGCTGGCGCAAGGGCAATTCCAGCAAGCGACTAATGGCAGCTCGGTGCCGTTCATCGAAAGCGTTGACGGCAGCGATTTCAAAGA                                                          <  SRR4302543.1573861/94‑1 (MQ=60)
                                    GCATGGCGGCGCTGGCGCAAGGGCAATTCCAGCAAGCGACTAATGGCAGCTCGGTGCCGTTCATCGAAAGCGTTGACGGCAGCGATTTCAAAGA                                                          <  SRR4302543.6843804/94‑1 (MQ=60)
                                    GCATGGCGGCGCTGGCGCAAGGGCAATTCCAGCAAGCGACTAATGGCAGCTCGGTGCCGTTCATCGAAAGCGTTGACGGCAGCGATTTCAAAGA                                                          >  SRR4302543.645825/1‑94 (MQ=60)
                                     CATGGCGGCGCTGGCGCAAGGGCAATTCCAGCAAGCGACTAATGGCAGCTCGGTGCCGTTCATCGAAAGCGTTGACGGCAGGGATTTCAAAGAT                                                         >  SRR4302543.3364166/1‑94 (MQ=60)
                                     CATGGCGGCGCTGGCGCAAGGGCAATTCCAGCAAGCGACTAATGGCAGCTCGGTGCCGTTCATCGAAAGCGTTGACGGCAGCGATTTCAAAGAT                                                         >  SRR4302543.3194858/1‑94 (MQ=60)
                                     CATGGCGGCGCTGGCGCAAGGGCAATTCCAGCAAGCGACTAATGGCAGCTCGGTGCCGTTCATCGAAAGCGTTGACGGCAGCGATTTCAAAGAT                                                         <  SRR4302543.5862244/94‑1 (MQ=60)
                                     CATGGCGGCGCTGGCGCAAGGGCAATTCCAGCAAGCGACTAATGGCAGCTCGGTGCCGTACATCGAAAGCGTTGACGGCAGCGATTTCAAAGAT                                                         <  SRR4302543.6987032/94‑1 (MQ=60)
                                      ATGGCGGCGCTGGCGCAAGGGCAATTCCAGCAAGCGACTAATGGCAGCTCGGTGCCGTTCATCGAAAGCGTTGACGGCAGCGATTTCAAAGATG                                                        >  SRR4302543.3383093/1‑94 (MQ=60)
                                      ATGGCGGCGCTGGCGCAAGGGCAATTCCAGCAAGCGACTAATGGCAGCTCGGTGCCGTTCATCGAAAGCGTTGACGGCAGCGATTTCAAAGATG                                                        >  SRR4302543.5828198/1‑94 (MQ=60)
                                       TGGCGGCGCTGGCGCAAGGGCAATTCCAGCAAGCGACTAATGGCAGCTCGGTGCCGTTCATCGAAAGCGTTGACGGCAGCGATTTCAAAGATGT                                                       >  SRR4302543.3561176/1‑94 (MQ=60)
                                       TGGCGGCGCTGGCGCAAGGGCAATTACAGCAAGCGACTAATGGCAGCTCGGTGAAGTTCATCGAAAGCGTTGACGGCAGCGATTTCAAAGATGT                                                       <  SRR4302543.4897199/94‑1 (MQ=60)
                                        GGCGGCGCTGGCGCAAGGGCAATTCCAGCAAGCGACTAATGGCAGCTCGGTGCCGTTCATCGAAAGCGTTGACGGCAGCGATTTCAAAGATGTG                                                      >  SRR4302543.5132166/1‑94 (MQ=60)
                                        GGCGGCGCTGGCGCAAGGGCAATTCCAGCAAGCGACTAATGGCAGCTCGGTGCCGTTCATCGAAAGCGTTGACGGCAGCGATTTCAAAGATGTG                                                      <  SRR4302543.7300004/94‑1 (MQ=60)
                                         GCGGCGCTGGCGCAAGGGCAATTCCAGCAAGCGACTAATGGCAGCTCGGTGCCGTTCATCGAAAGCGTTGACGGCAGCGATTTCAAAGATGTGT                                                     <  SRR4302543.6585141/94‑1 (MQ=60)
                                          CGGCGCTGGCGCAAGGGCAATTCCAGCAAGCGACTAATGGCAGCTCGGTGCCGTTCATCGAAAGCGTTGACGGCAGCGATTTCAAAGATGTGTT                                                    <  SRR4302543.1880816/94‑1 (MQ=60)
                                          CGGCGCTGGCGCAAGGGCAATTCCAGCAAGCGACTAATGGCAGCTCGGTGCCGTTCATCGAAAGCGTTGACGGCAGCGATTTCAAAGATGTGTT                                                    <  SRR4302543.153261/94‑1 (MQ=60)
                                          CGGCGCTGGCGCAAGGGCAATTCCAGCAAGCGACTAATGGCAGCTCGGTGCCGTTCATCGAAAGCGTTGACGGCAGCGATTTCAAAGATGTGTT                                                    >  SRR4302543.6783230/1‑94 (MQ=60)
                                           GGCGCTGGCGCAAGGGCAATTCCAGCAAGCGACTAATGGCAGCTCGGTGCCGTTCATCGAAAGCGTTGACGGCAGCGATTTCAAAGATGTGTTC                                                   >  SRR4302543.2365275/1‑94 (MQ=60)
                                            GCGCTGGCGCAAGGGCAATTCCAGCAAGCGACTAATGGCAGCTCGGTGCCGTTCATCGAAAGCGTTGACGGCAGCGATTTCAAAGATGTGTTCC                                                  <  SRR4302543.2516749/94‑1 (MQ=60)
                                             GGCTGGCGCAAGGGCAATTCCAGCAAGCGACTAATGGCAGCTCGGTGCCGTTCATCGAAAGCGTTGACGGCAGCGATTTCAAAGATGTGTTCCT                                                 <  SRR4302543.3171089/94‑1 (MQ=60)
                                             CGCTGGCGCAAGGGCAATTCCAGCAAGCGACTAATGGCAGCTCGGTGCCGTTCATCGAAAGCGTTGACGGCAGCGATTTCAAAGATGTGTTCCT                                                 >  SRR4302543.5127084/1‑94 (MQ=60)
                                             CGCTGGCGCAAGGGCAATTCCAGCAAGCGACTAATGGCAGCTCGGTGCCGTTCATCGAAAGCGTTGACGGCAGCGATTTCAAAGATGTGTTCCT                                                 >  SRR4302543.5711426/1‑94 (MQ=60)
                                              GCTGGCGCAAGGGCAATTCCAGCAAGCGACTAATGGCAGCTCGGTGCCGTTCATCGAAAGCGTTGACGGCAGCGATTTCAAAGATGTGTTCCTC                                                <  SRR4302543.2742015/94‑1 (MQ=60)
                                              GCTGGCGCAAGGGCAATTCCAGCAAGCGACTAATGGCAGCTCGGTGCCGTTCATCGAAAGCGTTGACGGCAGCGATTTCAAAGATGTGTTCCTC                                                >  SRR4302543.5733405/1‑94 (MQ=60)
                                               CTGGCGCAAGGGCAATTCCAGCAAGCGACTAATGGCAGCTCGGTGCCGTTCATCGAAAGCGTTGACGGCAGCGCTTTCAAAGAGGTGTTCCTCG                                               >  SRR4302543.2507151/1‑94 (MQ=60)
                                               CTGGCGCAAGGGCAATTCCAGCAAGCGACTAATGGCAGCTCGGTGCCGTTCATCGAAAGCGTTGACGGCAGCGATTTCAAAGATGTGTTCCTCG                                               >  SRR4302543.2507152/1‑94 (MQ=60)
                                               CTGGCGCAAGGGCAATTCCAGCAAGCGACTAATGGCAGCTCGGTGCCGTTCATCGAAAGCGTTGACGGCAGCGATTTCAAAGATGTGTTCCTCG                                               >  SRR4302543.3990229/1‑94 (MQ=60)
                                               CTGGCGCAAGGGCAATTCCAGCAAGCGACTAATGGCAGCTCGGTGCCGTTCATCGAAAGCGTTGACGGCAGCGATTTCAAAGATGTGTTCCTCG                                               <  SRR4302543.1188443/94‑1 (MQ=60)
                                                TGGCGCAAGGGCAATTCCAGCAAGCGACTAATGGCAGCTCGGTGCCGTTCATCGAAAGCGTTGACGGCAGCGATTTCAAAGATGTGTTCCTCGC                                              >  SRR4302543.4470991/1‑94 (MQ=60)
                                                   CGCAAGGGCAATTCCAGCAAGCGACTAATGGCAGCTCGGTGCCGTTCATCGAAAGCGTTGACGGCAGCGATTTCAAAGATGTGTTCCTCGCGCA                                           <  SRR4302543.7340262/94‑1 (MQ=60)
                                                    GCAAGGGCAATTCCAGCAAGCGACTAATGGCAGCTCGGTGCCGTTCATCGAAAGCGTTGACGGCAGCGATTTCAAAGATGTGTTCCTCGCGCAA                                          <  SRR4302543.7464712/94‑1 (MQ=60)
                                                     CAAGGGCAATTCCAGCAAGCGACTAATGGCAGCTCGGTGCCGTTCATCGAAAGCGTTGACGGCAGCGCTTTCAAAGATGTGTTCCTCGCGCAAA                                         >  SRR4302543.7673051/1‑94 (MQ=60)
                                                      AAGGGCAATTCCAGCAAGCGACTAATGGCAGCTCGGTGCCGTTCATCGAAAGCGTTGACGGCAGCGATTTCAAAGATGTGTTCCTCGCGCAAAT                                        >  SRR4302543.6785804/1‑94 (MQ=60)
                                                      AAGGGCAATTCCAGCAAGCGACTAATGGCAGCTCGGTGCCGTTCATCGAAAGCGTTGACGGCAGCGATTTCAAAGATGTGTTCCTCGCGCAAAT                                        >  SRR4302543.136810/1‑94 (MQ=60)
                                                      AAGGGCAATTCCAGCAAGCGACTAATGGCAGCTCGGTGCCGTTCATCGAAAGCGTTGACGGCAGCGATTTCAAAGATGTGTTCCTCGCGCAAAT                                        >  SRR4302543.6785803/1‑94 (MQ=60)
                                                      AAGGGCAATTCCAGCAAGCGACTAATGGCAGCTCGGTGCCGTTCATCGAAAGCGTTGACGGCAGCGATTTCAAAGATGTGTTACTCGCGCAAAT                                        <  SRR4302543.6719901/94‑1 (MQ=60)
                                                       AGGGCAATTCCAGCAAGCGACTAATGGCAGCTCGGTGCCGTTCATCGAAAGCGTTGACGGCAGCGATTTCAAAGATGTGTTCCTCGCGCAAATT                                       <  SRR4302543.808335/94‑1 (MQ=60)
                                                       AGGGCAATTCCAGCAAGCGACTAATGGCAGCTCGGTGCCGTTCATCGAAAGCGTTGACGGCAGCGATTTCAAAGATGTGTTCCTCGCGCAAATT                                       >  SRR4302543.6043396/1‑94 (MQ=60)
                                                        GGGCAATTCCAGCAAGCGACTAATGGCAGCTCGGTGCCGTTCATCGAAAGCGTTGACGGCAGCGATTTCAAAGATGTGTTCCTCGCGCAAATTC                                      <  SRR4302543.5826972/94‑1 (MQ=60)
                                                        GGGCAATTCCAGCAAGCGACTAATGGCAGCTCGGTGCCGTTCATCGAAAGCGTTGACGGCAGCGATTTCAAAGATGTGTTCCTCGCGCAAATTC                                      <  SRR4302543.5598681/94‑1 (MQ=60)
                                                        GGGCAATTCCAGCAAGCGACTAATGGCAGCTCGGTGCCGTTCATCGAAAGCGTTGACGGCAGCGATTTCAAAGATGTGTTCCTCGCGCAAATTC                                      <  SRR4302543.4555238/94‑1 (MQ=60)
                                                        GGGCAATTCCAGCAAGCGACTAATGGCAGCTCGGTGCCGTTCATCGAAAGCGTTGACGGCAGCGATTTCAAAGATGTGTTCCTCGCGCAAATTC                                      <  SRR4302543.1072501/94‑1 (MQ=60)
                                                         GGCAATTCCAGCAAGCGACTAATGGCAGCTCGGTGCCGTTCATCGAAAGCGTTGACGGCAGCGATTTCAAAGATGTGTTCCTCGCGCAAATTCG                                     <  SRR4302543.7594627/94‑1 (MQ=60)
                                                         GGCAATTCCAGCAAGCGACTAATGGCAGCTCGGTGCCGTTCATCGAAAGCGTTGACGGCAGCGATTTCAAAGATGTGTTCCTCGCGCAAATTCG                                     <  SRR4302543.2829392/94‑1 (MQ=60)
                                                          GCAATTCCAGCAAGCGACTAATGGCAGCTCGGTGCCGTTCATCGAAAGCGTTGACGGCAGCGATTTCAAAGATGTGTTCCTCGCGCAAATTCGA                                    <  SRR4302543.1133351/94‑1 (MQ=60)
                                                          GCAATTCCAGCAAGCGACTAATGGCAGCTCGGTGCCGTTCATCGAAAGCGTTGACGGCAGCGATTTCAAAGATGTGTTCCTCGCGCAAATTCGA                                    <  SRR4302543.7487261/94‑1 (MQ=60)
                                                           CAATTCCAGCAAGTGACTAATGGCAGCTCGGTGCCGTTCATCGAAAGCGTTGACGGCAGCGATTTCAAAGATGTGTTCCTCGCGCAAATTCGAC                                   >  SRR4302543.5906526/1‑94 (MQ=60)
                                                           CAATTCCAGCAAGCGACTAATGGCAGCTCGGTGCCGTTCATCGAAAGCGTTGACGGCAGCGATTTCAAAGATGTGTTCCTCGCGCAAATTCGAC                                   <  SRR4302543.3988517/94‑1 (MQ=60)
                                                           CAATTCCAGCAAGCGACTAATGGCAGCTCGGTGCCGTTCATCGAAAGCGTTGACGGAAGCGATTTCAAAGATGTTTTTCTCTCGCAAATTCGAC                                   >  SRR4302543.7046719/1‑94 (MQ=60)
                                                            AATTCCAGCAAGCGACTAATGGCAGCTCGGTGCCGTTCATCGAAAGCGTTGACGGCAGCGATTTCAAAGATGTGTTCCTCGCGCAAATTCGACC                                  >  SRR4302543.2524290/1‑94 (MQ=60)
                                                            AATTCCAGCAAGCGACTAATGGCAGCTCGGTGCCGTTCATCGAAAGCGTTGACGGCAGCGATTTCAAAGATGTGTTCCTCGCGCAAATTCGACC                                  <  SRR4302543.5884695/94‑1 (MQ=60)
                                                              attcaggAAGCGGCTAAAGGCAGCTCGGTGCCGTTCATCGAAAGCGTTGACGGCAGCGATTTCAAAGATGTGTTCCTCGCGCAAATTCGACCAA                                <  SRR4302543.7169433/87‑1 (MQ=60)
                                                              TTCCAGCAAGCGACTAATGGCAGCTCGGTGCCGTTCATCGAAAGCGTTGACGGCAGCGATTTCAAAGATGTGTTCCTCGCGCAAATTCGACCAA                                >  SRR4302543.7534261/1‑94 (MQ=60)
                                                               TCCAGCAAGCGACTAATGGCAGCTCGGTGCCGTTCATCGAAAGCGTTGCCGGCAGCGATTTCAAAGATGTGTTCCTCGCGCAAATTCGACCAAA                               <  SRR4302543.4866438/94‑1 (MQ=60)
                                                               TCCAGCAAGCGACTAATGGCAGCTCGGTGCCGTTCATCGAAAGCGTTGACGGCAGCGATTTCAAAGATGTGTTCCTCGCGCAAATTCGACCAAA                               >  SRR4302543.8011392/1‑94 (MQ=60)
                                                               TCCAGCAAGCGACTAATGGCAGCTCGGTGCCGTTCATCGAAAGCGTTGACGGCAGCGATTTCAAAGATGTGTTCCTCGCGCAAATTCGACCAAA                               >  SRR4302543.7468850/1‑94 (MQ=60)
                                                               TCCAGCAAGCGACTAATGGCAGCTCGGTGCCGTTCATCGAAAGCGTTGACGGCAGCGATTTCAAAGATGTGTTCCTCGCGCAAATTCGACCAAA                               >  SRR4302543.1220703/1‑94 (MQ=60)
                                                                CCAGCAAGCGACTAATGGCAGCTCGGTGCCGTTCATCGAAAGCGTTGACGGCAGCGATTTCAAAGATGTGTTCCTCGCGCAAATTCGACCAAAA                              >  SRR4302543.6551884/1‑94 (MQ=60)
                                                                CCAGCAAGCGACTAATGGCAGCTCGGTGCCGTTCATCGAAAGCGTTGACGGCAGCGATTTCAAAGATGTGTTCCTCGCGCAAATTCGACCAAAA                              <  SRR4302543.7800134/94‑1 (MQ=60)
                                                                 CAGCAAGCGACTAATGGCAGCTCGGTGCCGTTCATCGAAAGCGTTGACGGCAGCGATTTCAAAGATGTGTTCCTCGCGCAAATTCGACCAAAAG                             >  SRR4302543.804010/1‑94 (MQ=60)
                                                                 CAGCAAGCGACTAATGGCAGCTCGGTGCCGTTCATCGAAAGCGTTGACGGCAGCGATTTCAAAGATGTGTTCCTCGCGCAAATTCGACCAAAAG                             <  SRR4302543.4134729/94‑1 (MQ=60)
                                                                 CAGCAAGCGACTAATGGCAGCTCGGTGCCGTTCATCGAAAGCGTTGACGGCAGCGATTTCAAAGATGTGTTCCTCGCGCAAATTCGACCAAAAG                             <  SRR4302543.6664451/94‑1 (MQ=60)
                                                                 CAGCAAGCGACTAATGGCAGCTCGGTGCCGTTCATCGAAAGCGTTGACGGCAGCGATTTCAAAGATGTGTTCCTCGCGCAAATTCGACCAAAAG                             >  SRR4302543.3585860/1‑94 (MQ=60)
                                                                  AGCAAGCGACTAATGGCAGCTCGGTGCCGTTCATCGAAAGCGTTGACGGCAGCGATTTCAAAGATGTGTTCCTCGCGCAAATTCGACCAAAAGG                            <  SRR4302543.6714984/94‑1 (MQ=60)
                                                                  AGCAAGCGACTAATGGCAGCTCGGTGCCGTTCATCGAAAGCGTTGACGGCAGCGATTTCAAAGATGTGTTCCTCGCGCAAATTCGACCAAAAGG                            >  SRR4302543.4939631/1‑94 (MQ=60)
                                                                  AGCAAGCGACTAATGGCAGCTCGGTGCCGTTCATCGAAAGCGTTGACGGCAGCGATTTCAAAGATGTGTTCCTCGCGCAAATTCGACCAAAAGG                            <  SRR4302543.792427/94‑1 (MQ=60)
                                                                  AGCAAGCGACTAATGGCAGCTCGGTGCCGTTCATCGAAAGCGTTGACGGCAGCGATTTCAAAGATGTGTTCCTCGCGCAAATTCGACCAAAAGG                            <  SRR4302543.5288707/94‑1 (MQ=60)
                                                                  AGCAAGCGACTAATGGCAGCTCGGTGCCGTTCATCGAAAGCGTTGACGGCAGCGATTTCAAAGATGTGTTCCTCGCGCAAATTCGACCAAAAGG                            >  SRR4302543.4939630/1‑94 (MQ=60)
                                                                  AGCAAGCGACTAATGGCAGCTCGGTGCCGTTCATCGAAAGCGTTGACGGCAGCGATTTCAAAGATGTGTTCCTCGCGCAAATTCGACCAAAAGG                            <  SRR4302543.5114641/94‑1 (MQ=60)
                                                                   GCAAGCGACTAATGGCAGCTCGGTGCCGTTCATCGAAAGCGTTGACGGCAGCGATTTCAAAGATGTGTTCCTCGCGCAAATTCGACCAAAAGGT                           >  SRR4302543.35251/1‑94 (MQ=60)
                                                                    CAAGCGACTAATGGCAGCTCGGTGCCGTTCATCGAAAGCGTTGACGGCAGCGATTTCAAAGATGTGTTCCTCGCGCAAATTCGACCAAAAGGTA                          <  SRR4302543.2289467/94‑1 (MQ=60)
                                                                     AAGCGACTAATGGCAGCTCGGTGCCGTTCATCGAAAGCGTTGACGGCAGCGATTTCAAAGATGTGTTCCTCGCGCAAATTCGACCAAAAGGTAA                         <  SRR4302543.4144697/94‑1 (MQ=60)
                                                                     AAGCGACTAATGGCAGCTCGGTGCCGTTCATCGAAAGCGTTGACGGCAGCGATTTCAAAGATGTGTTCCTCGCGCAAATTCGACCAAAAGGTAA                         <  SRR4302543.3238046/94‑1 (MQ=60)
                                                                      AGCGACTAATGGCAGCTCGTTGCCGTTCATCGAAAGCGTTGACGGCAGCGATTTCAAAGATGTGTTCCTCGCGCAAATTCGACCAAAAGGTAAT                        <  SRR4302543.6640355/94‑1 (MQ=60)
                                                                      AGCGACTAATGGCAGCTCGGTGCCGTTCATCGAAAGCGTTGACGGCAGCGATTTCAAAGATGTGTTCCTCGCGCAAATTCGACCAAAAGGTAAT                        <  SRR4302543.4762370/94‑1 (MQ=60)
                                                                       GCGACTAATGGCAGCTCGGTGCCGTTCATCGAAAGCGTTGACGGCAGCGATTTCAAAGATGTGTTCCTCGCGCAAATTCGACCAAAAGGTAATG                       >  SRR4302543.4740242/1‑94 (MQ=60)
                                                                        CGACTAATGGCAGCTCGGTGCCGTTCATCGAAAGCGTTGACGGCAGCGATTTCAAAGATGTGTTCCTCGCGCAAATTCGACCGAAAGGTAATGC                      >  SRR4302543.1330455/1‑94 (MQ=60)
                                                                        CGACTAATGGCAGCTCGGTGCCGTTCATCGAAAGCGTTGACGGCAGCGATTTCAAAGATGTGTTCCTCGCGCAAATTCGACCAAAAGGTAATGC                      >  SRR4302543.4110878/1‑94 (MQ=60)
                                                                        CGACTAATGGCAGCTCGGTGCCGTTCATCGAAAGCGTTGACGGCAGCGATTTCAAAGATGTGTTCCTCGCGCAAATTCGACCAAAAGGTAATGC                      <  SRR4302543.1364260/94‑1 (MQ=60)
                                                                        CGACTAATGGCAGCTCGGTGCCGTTCATCGAAAGCGTTGACGGCAGCGATTTCAAAGATGTGTTCCTCGCGCAAATTCGACCAAAAGGTAATGC                      >  SRR4302543.988360/1‑94 (MQ=60)
                                                                        CGACTAATGGCAGCTCGGTGCCGTTCATCGAAAGCGTTGACGGCAGCGATTTCAAAGATGTGTTCCTCGCGCAAATTCGACCAAAAGGCAATGC                      >  SRR4302543.5706833/1‑94 (MQ=60)
                                                                          ACTAATGGCAGCTCGGTGCCGTTCATCGAAAGCGTTGACGGCAGCGATTTCAAAGATGTGTTCCTCGCGCAAATTCGACCAAAAGGTAATGCAC                    >  SRR4302543.5132061/1‑94 (MQ=60)
                                                                          ACTAATGGCAGCTCGGTGCCGTTCATCGAAAGCGTTGACGGCAGCGATTTCAAAGATGTGTTCCTCGCGCAAATTCGACCAAAAGGTAATGCAC                    >  SRR4302543.5132062/1‑94 (MQ=60)
                                                                          ACTAATGGCAGCTCGGTGCCGTTCATCGAAAGCGTTGACGGCAGCGATTTCAAAGATGTGTTCCTCGCGCAAATTCGACCAAAAGGTAATGCAC                    <  SRR4302543.2782622/94‑1 (MQ=60)
                                                                           CTAATGGCAGCTCGGTGCTGCTCATCGAAAGGGTTGACGGCAGCGATTTCAAGGATGTGTTCCGCGCGCAcatcgctccaaagggaaatgcgct                   >  SRR4302543.2715512/1‑70 (MQ=60)
                                                                           CTAATGGCAGCTCGGTGCCGTTCATCGAAAGCGTTGACGGCAGCGATTTCAAAGATGTGTTCCTCGCGCAAATTCGACCAAAAGGTAATGCACG                   <  SRR4302543.40515/94‑1 (MQ=60)
                                                                              ATGGCAGCTCGGTGCCGTTCATCGAAAGCGTTGACGGCAGCGATTTCAAAGATGTGTTCCTCGCGCAAATTCGACCAAAAGGTAATGCACGTCC                >  SRR4302543.3590553/1‑94 (MQ=60)
                                                                              ATGGCAGCTCGGTGCCGTTCATCGAAAGCGTTGACGGCAGCGATTTCAAAGATGTGTTCCTCGCGCAAATTCGACCAAAAGGTAATGCACGTCC                >  SRR4302543.3398056/1‑94 (MQ=60)
                                                                              ATGGCAGCTCGGTGCCGTTCATCGAAAGCGTTGACGGCAGCGATTTCAAAGATGTGTTCCTCGCGCAAATTCGACCAAAAGGTAATGCACGTCC                >  SRR4302543.3420784/1‑94 (MQ=60)
                                                                              ATGGCAGCTCGGTGCCGTTCATCGAAAGCGTTGACGGCAGCGATTTCAAAGATGTGTTCCTCGCGCAAATTCGACCAAAAGGTAATGCACGTCC                <  SRR4302543.2555759/94‑1 (MQ=60)
                                                                              ATGGCAGCTCGGTGCCGTTCATCGAAAGCGTTGACGGCAGCGATTTCAAAGATGTGTTCCTCGCGCAAATTCCACCAAAAGGTAATGCACGTCC                >  SRR4302543.5710234/1‑94 (MQ=60)
                                                                               TGGCAGCTCGGTGCCGTTCATCGAAAGCGTTGACGGCAGCGATTTCAAAGATGTGTTCCTCGCGCAAATTCGACCAAAAGGTCATGCACGTCCT               >  SRR4302543.4830603/1‑94 (MQ=60)
                                                                               TGGCAGCTCGGTGCCGTTCATCGAAAGCGTTGACGGCAGCGATTTCAAAGATGTGTTCCTCGCGCAAATTCGACCAAAAGGTAATGCACGTCCT               <  SRR4302543.547268/94‑1 (MQ=60)
                                                                                GGCAGCTCGGTGCCGTTCATCGAAAGCGTTGACGGCAGCGATTTCAAAGATGTGTTCCTCGCGCAAATTCGACCAAAAGGTAATGCACGTCCTT              <  SRR4302543.79079/94‑1 (MQ=60)
                                                                                GGCAGCTCGGTGCCGTTCATCGAAAGCGTTGACGGCAGCGATTTCAAAGATGTGTTCCTCGCGCAAATTCGACCAAAAGGTAATGCACGTCCTT              <  SRR4302543.79080/94‑1 (MQ=60)
                                                                                  CAGCTCGGTGCCGTTCATCGAAAGCGTTGACGGCAGCGATTTCAAAGATGTGTTCCTCGCGCAAATTCGACCAAAAGGTAATGCACGTCCTTCT            <  SRR4302543.294741/94‑1 (MQ=60)
                                                                                  CAGCTCGGTGCCGTTCATCGAAAGCGTTGACGGCAGCGATTTCAAAGATGTGTTCCTCGCGCAAATTCGACCAAAAGGTAATGCACGTCCTTCT            <  SRR4302543.1832955/94‑1 (MQ=60)
                                                                                  CAGCTCGGTGCCGTTCATCGAAAGCGTTGACGGCAGCGATTTCAAAGATGTGTTCCTCGCGCAAATTCGACCAAAAGGTAATGCACGTCCTTCT            >  SRR4302543.3429514/1‑94 (MQ=60)
                                                                                  CAGCTCGGTGCCGTTCATCGAAAGCGTTGACGGCAGCGATTTCAAAGATGTGTTCCTCGCGCAAATTCGACCAAAAGGTAATGCACGTCCTTCT            >  SRR4302543.5853153/1‑94 (MQ=60)
                                                                                  CAGCTCGGTGCCGTTCATCGAAAGCGTTGACGGCAGCGATTTCAAAGATGTGTTCCTCGCGCAAATTCGACCAAAAGGTAATGCACGTCCTTCT            <  SRR4302543.4846733/94‑1 (MQ=60)
                                                                                   AGCTCGGTGCCGTTCATCGAAAGCGTTGACGGCAGCGATTTCAAAGATGTGTTCCTCGCGCAAATTCGACCAAAAGGTAATGCACGTCCTTCTG           >  SRR4302543.3457509/1‑94 (MQ=60)
                                                                                   AGCTCGGTGCCGTTCATCGAAAGCGTTGACGGCAGCGATTTCAAAGATGTGTTCCTCGCGCAAATTCGACCAAAAGGTAATGCACGTCCTTCTG           >  SRR4302543.2386442/1‑94 (MQ=60)
                                                                                   AGCTCGGTGCCGTTCATCGAAAGCGTTGACGGCAGCGATTTCAAAGATGTGTTCCTCGCGCAAATTCGACCAAAAGGTAATGAACGTCCTTCTG           >  SRR4302543.6625961/1‑94 (MQ=60)
                                                                                    GCTCGGTGCCGTTCATCGAAAGCGTTGACGGCAGCGATTTCAAAGATGTGTTCCTCGCGCAAATTCGACCAAAAGGTAATGCACGTCCTTCTGT          <  SRR4302543.2598470/94‑1 (MQ=60)
                                                                                    GCTCGGTGCCGTTCATCGAAAGCGTTGACGGCAGCGATTTCAAAGATGTGTTCCTCGCGCAAATTCGACCAAAAGGTAATGCACGTCCTTCTGT          <  SRR4302543.4403501/94‑1 (MQ=60)
                                                                                    GCTCGGTGCCGTTCATCGAAAGCGTTGACGGCAGCGATTTCAAAGATGTGTTCCTCGCGCAAATTCGACCAAAAGGTAATGCACGTCCTTCTGT          <  SRR4302543.2465971/94‑1 (MQ=60)
                                                                                     CTCGGTGCCGTTCATCGAAAGCGTTGACGGCAGCGATTTCAAAGATGTGTTCCTCGCGCAAATTCGCCCAAACGGTAATGCACGTCCTTCTGTG         >  SRR4302543.2145380/1‑94 (MQ=60)
                                                                                     CTCGGTGCCGTTCATCGAAAGCGTTGACGGCAGCGATTTCAAAGATGTGTTCCTCGCGCAAATTCGACCAAAAGGTAATGCACGTCCTTCTGTG         >  SRR4302543.2755318/1‑94 (MQ=60)
                                                                                     CTCGGTGCCGTTCATCGAAAGCGTTGACGGCAGCGATTTCAAAGATGTGTTCCTCGCGCAAATTCGACCAAAAGGTAATGCACGTCCTTCTGTG         <  SRR4302543.7779367/94‑1 (MQ=60)
                                                                                     CTCGGTGCCGTTCATCGAAAGCGTTGACGGCAGCGATTTCAAAGATGTGTTCCTCGCGCAAATTCGACCAAAAGGTAATGCACGTCCTTCTGTG         >  SRR4302543.468787/1‑94 (MQ=60)
                                                                                     CTCGGTGCCGTTCATCGAAAGCGTTGACGGCAGCGATTTCAAAGATGTGTTCCTCGCGCAAATTCGACCAAAAGGTAATGCACGTCCTTCTGTG         >  SRR4302543.358603/1‑94 (MQ=60)
                                                                                       CGGTGCCGTTCATCGAAAGCGTTGACGGCAGCGATTTCAAAGATGTGTTCCTCGCGCAAATTCGACCAAAAGGTAATGCACGTCCTTCTGTGGG       >  SRR4302543.5756275/1‑94 (MQ=60)
                                                                                        GGTGCTGTTCATCGAAAGCGTTGACGGCAGCGATTTCAAAGATGTGTTCCTCGCGCAAATTCGACCAAAAGGTAATGCACGTCCTTCTGTGGTG      >  SRR4302543.3138037/1‑94 (MQ=60)
                                                                                        GGTGCCGTTCATCGAAAGCGTTGACGGCAGCGATTTCAAAGATGTGTTCCTCGCGCAAATTCGACCAAAAGGTAATGCACGTCCTTCTGTGGTG      >  SRR4302543.5829939/1‑94 (MQ=60)
                                                                                        GGTGCCGTTCATCGAAAGCGTTGACGGCAGCGATTTCAAAGATGTGTTCCTCGCGCAAATTCGACCAAAAGGTAATGCACGTCCTTCTGTGGTG      >  SRR4302543.7550605/1‑94 (MQ=60)
                                                                                        GGTGCCGTTCATCGAAAGCGTTGACGGCAGCGATTTCAAAGATGTGTTCCTCGCGCAAATTCGACCAAAAGGTAATGCACGTCCTTCTGTGGTG      >  SRR4302543.809157/1‑94 (MQ=60)
                                                                                        GGTGCCGTTCATCGAAAGCGTTGACGGCAGCGATTTCAAAGATGTGTTCCTCGCGCAAATTCGACCAAAAGGTAATGCACGTCCTTCTGTGGTG      >  SRR4302543.1918785/1‑94 (MQ=60)
                                                                                         GTGCCGTTCATCGAAAGCGTTGACGGCAGCGATTTCAAAGATGTGTTCCTCGCGCAAATTCGACCAAAAGGTAATGCACGTCCTTCTGTGGTGG     >  SRR4302543.3179313/1‑94 (MQ=60)
                                                                                          TGCCGTTCATCGAAAGCGTTGACGGCAGCGATTTCAAAGATGTGTTCCTCGCGCAAATTCGACCAAAAGGTAATGCACGTCCTTCTGTGGTGGT    >  SRR4302543.1851332/1‑94 (MQ=60)
                                                                                          TGCCGTTCATCGAAAGCGTTGACGGCAGCGATTTCAAAGATGTGTTCCTCGCGCAAATTCGACCAAAAGGTAATGCACGTCCTTCTGTGGTGGT    <  SRR4302543.5255380/94‑1 (MQ=60)
                                                                                           GCCGTTCATCGAAAGCGTTGACGGCAGCGATTTCAAAGATGTGTTCCTCGCGCAAATTCGACCAAAAGGTAATGCACGTCCTTCTGTGGTGGTG   <  SRR4302543.7917765/94‑1 (MQ=60)
                                                                                           GCCGTTCATCGAAAGCGTTGACGGCAGCGATTTCAAAGATGTGTTCCTCGCGCAAATTCGACCAAAAGGTAATGCACGTCCTTCTGTGGTGGTG   >  SRR4302543.4857611/1‑94 (MQ=60)
                                                                                           GCCGTTCATCGAAAGCGTTGACGGCAGCGATTTCAAAGATGTGTTCCTCGCGCAAATTCGACCAAAAGGTAATGCACGTCCTTCTGTGGTGGTG   >  SRR4302543.3713421/1‑94 (MQ=60)
                                                                                            CCGTTCATCGAAAGCGTTGACGGCAGCGATTTCAAAGATGTGTTCCTCGCGCAAATTCGACCAAAAGGTAATGCACGTCCTTCTGTGGTGGTGG  <  SRR4302543.2167228/94‑1 (MQ=60)
                                                                                            CCGTTCATCGAAAGCGTTGACGGCAGCGATTTCAAAGATGTGTTCCTCGCGCAAATTCGACCAAAAGGTAATGCACGTCCTTCTGTGGTGGTGG  <  SRR4302543.1513904/94‑1 (MQ=60)
                                                                                            CCGTTCATCGAAAGCGTTGACGGCAGCGATTTCAAAGATGTGTTCCTCGCGCAAATTCGACCAAAAGGTAATGCACGTCCTTCTGTGGTGGTGG  <  SRR4302543.7738647/94‑1 (MQ=60)
                                                                                            CCGTTCATCGAAAGCGTTGACGGCAGCGATTTCAAAGATGTGTTCCTCGCGCAAATTCGACCAAAAGGTAATGCACGTCCTTCTGTGGCGGTGG  <  SRR4302543.3866629/94‑1 (MQ=60)
                                                                                             |                                                                                            
AGGATGAAGTGTTAGCAGAAGCGAAAGCGAACCCTGGCATGGCGGCGCTGGCGCAAGGGCAATTCCAGCAAGCGACTAATGGCAGCTCGGTGCTGTTCATCGAAAGCGTTGACGGCAGCGATTTCAAAGATGTGTTCCTCGCGCAAATTCGACCAAAAGGTAATGCACGTCCTTCTGTGGTGGTGG  >  NC_000913/4484168‑4484353

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑