Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I1 R1	1696	279.3	15874187	97.8%	15524954	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,603,785	A→G	noncoding (71/87 nt)	leuP ←	tRNA‑Leu

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,603,785	0	A	G	94.1%	610.3 / 32.7	222	noncoding (71/87 nt)	leuP	tRNA‑Leu
Reads supporting (aligned to +/- strand): ref base A (0/0); major base G (123/86); minor base . (7/6); total (130/92)
Fisher's exact test for biased strand distribution p-value = 7.76e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.24e-01

Only 100 of 240 total aligned reads displayed.

CACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑GA‑‑‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAA  >  NC_000913/4603695‑4603874

 acCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑GA‑‑‑GGGGGGGGGCtt                                                                                        >  1:2810229/1‑93 (MQ=255)

  ccTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑GA‑‑‑GGGGGGGGGCTTg                                                                                       >  2:5211662/1‑93 (MQ=255)

        gCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑GA‑‑‑GGGGGGGGGCTTGAAcccc                                                                                 <  1:3347396/93‑1 (MQ=255)

          tAGCGCGTCTCCCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑GA‑‑‑GGGGGGGGGCTTGAACCCCCa                                                                               <  2:6136675/93‑1 (MQ=255)

          tAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑GA‑‑‑GGGGGGGGGCTTGAACCCCCa                                                                               <  1:530402/93‑1 (MQ=255)

          tAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑GA‑‑‑GGGGGGGGGCTTGAACCCCCa                                                                               <  2:4864766/93‑1 (MQ=255)

          tAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑GA‑‑‑GGGGGGGGGCTTGAACCCCCa                                                                               <  2:3132673/93‑1 (MQ=255)

          tAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑GA‑‑‑GGGGGGGG‑CTTGAACCCCCAc                                                                              >  1:7667984/1‑93 (MQ=255)

           aGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑GAG‑‑GGGGGGGGGCTTGAACCCCCa                                                                               <  1:1191476/93‑1 (MQ=255)

            gcgcGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑GAGG‑GGGGGGGGGCTTGAAcccccc                                                                               >  1:1507250/1‑92 (MQ=38)

            gcgcGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑GA‑‑‑GGGGGGGGGCTTGAACCCCCACg                                                                             <  2:7334840/93‑1 (MQ=255)

            gcgcGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑GA‑‑‑GGGGGGGGGCTTGAACCCCCACg                                                                             >  1:2997477/1‑93 (MQ=255)

              gcgTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑GA‑‑‑GGGGGGGGGCTTGAACCCCCACGTc                                                                           >  1:3918854/1‑93 (MQ=255)

                 tCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑GAG‑‑GGGGGGGGGCTTGAACCCCCACGTCCg                                                                         >  1:4152122/1‑93 (MQ=255)

                 tCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑GAG‑‑GGGGGGGGGCTTGAACCCCCACGTCCg                                                                         >  2:2144802/1‑93 (MQ=255)

                 tCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑GA‑‑‑GGGGGGGGGCTTGAACCCCCACGTCCga                                                                        >  1:2347294/1‑92 (MQ=255)

                 tCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑GA‑‑‑GGGGGGGGGCTTGAACCCCCACGTCCGt                                                                        >  2:4713456/1‑93 (MQ=255)

                  ctcccAATGCCGCCCCCTTCGCACAGTCATCTTTCTTTTTTTGATATCGCCTTGTTTGGGGCGGG‑‑‑GGGGGGGGGCTTGAACCCCCACGTCCGt                                                                        <  1:729487/90‑1 (MQ=25)

                   tACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑GAGG‑GGGGGGGGGCTTGAACCCCCACGTCCga                                                                        >  2:3651678/1‑92 (MQ=38)

                    gccAATTCCCCCACTTTCGCAACGTCATCTTCTTTTTTTTGATATCGATTGGTTTGGTGC‑GAG‑‑GGGGGGGGGCTTGAACCCCCACGTCCGTaa                                                                      <  2:3921913/92‑1 (MQ=16)

                      cAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑GA‑‑‑GGGGGGGGGCTTGAACCCCCACGTCCGTAAGaa                                                                   <  2:6511433/93‑1 (MQ=255)

                       aaTTCCGCCCCCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑GAG‑‑GGGGGGGGGCTTGAACCCCCACGTCCGTAAGaa                                                                   <  1:2313097/93‑1 (MQ=37)

                       aaTTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑GA‑‑‑GGGGGGGGGCTTGAACCCCCACGTCCGTAAGAac                                                                  <  1:2562922/93‑1 (MQ=255)

                       aaTTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑GA‑‑‑GGGGGGGGGCTTGAACCCCCACGTCCGTAAGAac                                                                  >  1:5056714/1‑93 (MQ=255)

                        aTTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑GAG‑‑GGGGGGGGGCTTGAACCCCCACGTCCGTAAGAac                                                                  <  1:478591/93‑1 (MQ=255)

                         ttCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑GA‑‑‑GGGGGGGGGCTTGAACCCCCACGTCCGTAAGAacac                                                                >  2:314682/1‑93 (MQ=255)

                          tCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑GAGG‑GGGGGGGGGCTTGAACCCCCACGTCCGTAAGAaca                                                                 <  1:1027692/93‑1 (MQ=38)

                           ccGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑GAG‑‑GGGGGGGGGCTTGAACCACCACGTCCGTAGGAacaca                                                               >  2:7911583/1‑92 (MQ=37)

                            cGCCCCCTTCGAGAAGTCATCTTAGGTTTTTTGAGAGCGCCTCGTTTGTTGC‑GAG‑‑GGGGGGGGGCTTGAACCCCCACGTCCGTAAGAACACTa                                                              <  2:1190456/93‑1 (MQ=16)

                            cGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑GA‑‑‑GGGGGGGGGCTTGAACCCCCACGTCCGTAAGAACACTaa                                                             >  1:1401383/1‑93 (MQ=255)

                             gCCCCCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑GA‑‑‑GGGGGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAac                                                            <  1:324321/93‑1 (MQ=255)

                             gCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑GAG‑‑GGGGGGGGGCTTGAACCCCCACGTCCGTAAGAACACTaa                                                             >  2:6985575/1‑93 (MQ=255)

                             gCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑GA‑‑‑GGGGGGGG‑CTTGAACCCCCACGTCCGTAAGAACACTAaca                                                           >  2:2365216/1‑93 (MQ=255)

                             ccctccTTCTCACAGTCATCTTTCTTTTTTTGATAGCGCCTGGTTTGGTGC‑AAG‑‑GGGGGGGGGCTTGACCCCCCACGTCCGTAAGAACACTaa                                                             <  1:6552603/89‑1 (MQ=21)

                             ccccccTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑GAG‑‑GGGGGGGGGCTTGAACCCCCACGTCCGTAAGAACACTaa                                                             <  2:1334469/89‑1 (MQ=37)

                               cACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑GA‑‑‑GGGGGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAacac                                                          >  2:6597885/1‑93 (MQ=255)

                                aCCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑GAG‑‑GGGGGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAacac                                                          >  2:7811524/1‑93 (MQ=255)

                                aCCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑GA‑‑‑GGGGGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAACAcc                                                         >  2:5969793/1‑93 (MQ=255)

                                 ccTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑GAG‑‑GGGGGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAACAcc                                                         >  1:3381192/1‑93 (MQ=255)

                                 ccTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑GAG‑‑GGGGGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAACAcc                                                         >  2:6230679/1‑93 (MQ=255)

                                 ccTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑GA‑‑‑GGGGGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAACACCt                                                        >  2:788565/1‑93 (MQ=255)

                                  cTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑GAG‑‑GGGGGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAACACCt                                                        >  1:5506960/1‑93 (MQ=255)

                                  cTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑GAG‑‑GGGGGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAACACCt                                                        >  1:1254826/1‑93 (MQ=255)

                                   ttCGCACAGTCATTTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑GA‑‑‑GGGGGGGGGCTTGACCCCCCACGTCCGTAAGAACACTAACACCTGa                                                      <  2:1416829/93‑1 (MQ=255)

                                   ttCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑GAGG‑GGGGGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAACACCt                                                        >  1:4992687/1‑93 (MQ=25)

                                   ttCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑GA‑‑‑GGGGGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAACACCTGa                                                      >  2:2995563/1‑93 (MQ=255)

                                   ttCGCAAAGTAATTTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑GA‑‑‑GGGGGGGGGCTTGACCCCCCACGTCCGTAAGAACACTAACACCTGa                                                      <  1:3078092/93‑1 (MQ=21)

                                    tCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑GA‑‑‑GGGGGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAACACCTGaa                                                     <  2:51517/93‑1 (MQ=255)

                                      ccacaGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑GA‑‑‑GGGGGGGG‑CTTGACCCCCCACGTCCGTAAGAACACTAACACCTGAAGCt                                                  <  2:4378055/92‑1 (MQ=255)

                                         caGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑GAG‑‑GGGGGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTa                                                 <  2:5340805/93‑1 (MQ=255)

                                         caGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑GA‑‑‑GGGGGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAACACGTGAAACTAg                                                >  2:7712995/1‑93 (MQ=255)

                                          aGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑GA‑‑‑GGGGGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAgc                                               <  1:7920463/93‑1 (MQ=255)

                                          aGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑GA‑‑‑GGGGGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAgc                                               >  2:7158003/1‑93 (MQ=255)

                                           gTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑GAG‑‑GGGGGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAgc                                               >  1:7762948/1‑93 (MQ=255)

                                           gTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑GAG‑‑GGGGGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAACACCTAAAGCTAgg                                               >  2:1808856/1‑92 (MQ=21)

                                           gTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑GA‑‑‑GGGGGGGG‑CTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAgcgc                                             >  1:3898473/1‑93 (MQ=255)

                                            tCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑GAGG‑GGGGGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAgc                                               >  1:7846938/1‑93 (MQ=21)

                                              aTCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑GAGGGGGGGGGGGGCTTGAACCCCCCCGTCCGTAAGAACACTAACCCCTGAAGCTAgcg                                              >  2:3621920/1‑93 (MQ=21)

                                                 tttcTTTTTTTGATTTCGCCTCGTTTGGTGC‑GA‑‑‑GGGGGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTa                                        <  1:1661957/90‑1 (MQ=255)

                                                 ttACTTTTTTTGATATCGCCTCGTTTGGTGC‑GAG‑‑GGGGGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCt                                         >  1:1103098/1‑93 (MQ=255)

                                                  tACTTTTTTTGATATCGCCTCGTTTGGTGC‑GA‑‑‑GGGGGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTAc                                       >  2:4906783/1‑93 (MQ=255)

                                                  tACTTTTTTTGATATCGCCTCGTTTGGTGC‑GA‑‑‑GGGGGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTAc                                       <  1:3302777/93‑1 (MQ=255)

                                                  tACTTTTTTTGATATCGCCTCGTTTGGTGC‑GA‑‑‑GGGGGGGG‑CTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTAcc                                      >  1:4706506/1‑93 (MQ=255)

                                                   aCTTTTTTTGATATCGCCTCGTTTGGTGC‑GA‑‑‑GGGGGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTAcc                                      >  1:6128684/1‑93 (MQ=255)

                                                    cTTTTTTTGATATCGTCTCGTTTGGTGC‑GA‑‑‑GGGGGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCa                                     <  2:4178303/93‑1 (MQ=255)

                                                    cTTTTTTTGATATCGCCTCGTTTGGTGC‑GA‑‑‑GGGGGGGG‑CTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCaa                                    >  2:1727476/1‑93 (MQ=255)

                                                       tttttGATATCGCCTCGTTTGGTGC‑GAG‑‑GGGGGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAAt                                   >  1:6298635/1‑93 (MQ=255)

                                                        ttttTATAGCGCCTCGTTTGGTGC‑GA‑‑‑GGGGGGGG‑CTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTcc                                <  1:2666706/93‑1 (MQ=14)

                                                        ttttGATATCGCCTCGTTTGGTGC‑GA‑‑‑GGGGGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTc                                 <  1:4403182/93‑1 (MQ=255)

                                                         tttGATGTCGCCTCGTTTGGTGG‑GA‑‑‑GGGGGGGGGCTTGACCCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTcc                                <  1:1875493/93‑1 (MQ=17)

                                                         tttGATATCGCCGCGTTTGGGGC‑GAG‑‑GGGGGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTc                                 <  2:1024788/93‑1 (MQ=17)

                                                          ttGATATCGCCTCGTTTGGTGC‑GA‑‑‑GGGGGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCg                               >  2:2173676/1‑93 (MQ=255)

                                                          ttGATATCGCCTCGTTTGGTGC‑GA‑‑‑GGGGGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCg                               >  1:5580663/1‑93 (MQ=255)

                                                           tGATATCGCCTCGTTTGGTGC‑GAG‑‑GGGGGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTAGCAATTCCg                               >  1:5778920/1‑93 (MQ=17)

                                                               atCGCCTCGTTTGGTGC‑GAGG‑GGGGGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGccc                            >  2:1113005/1‑92 (MQ=14)

                                                               atCGCCTCGTTTGGTGC‑GAG‑‑GGGGGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCAc                           >  1:6749110/1‑93 (MQ=255)

                                                                tCGCCTCGTTTGGTGC‑GAG‑‑GGGGGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCAcc                          >  1:1990816/1‑93 (MQ=255)

                                                                tCGCCTCGTTTGGTGC‑GA‑‑‑GGGGGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCt                         >  1:1770208/1‑93 (MQ=255)

                                                                 cGCCTCGTTTGGTGC‑GA‑‑‑GGGGGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCtt                        <  1:7580075/93‑1 (MQ=255)

                                                                  gCCTCGTTTGGTGC‑GAG‑‑GGGGGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCtt                        >  2:4484297/1‑93 (MQ=255)

                                                                  gCCTCGTTTGGTGC‑GAG‑‑GGGGGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCtt                        >  2:2285025/1‑93 (MQ=255)

                                                                  gCCTCGTTTGGTGC‑GA‑‑‑GGGGGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTc                       >  1:108876/1‑93 (MQ=255)

                                                                  gCCTCGTTTGGTGC‑GA‑‑‑GGGGGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTc                       >  1:3440807/1‑93 (MQ=255)

                                                                  gCCTCGTTTGGTGC‑GA‑‑‑GGGGGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCAACTTc                       >  2:812115/1‑93 (MQ=255)

                                                                     tCGTTTGGTGC‑GAGG‑GGGGGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCg                      >  2:20139/1‑93 (MQ=11)

                                                                     tCGTTTGGTGC‑GAG‑‑GGGGGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGc                     >  2:2743636/1‑93 (MQ=255)

                                                                     tCGTTTGGTGC‑GAG‑‑GGGGGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGc                     >  1:4061670/1‑93 (MQ=255)

                                                                       gTTTGGTGC‑GA‑‑‑GGGGGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTAGCAATTCCGCCACCTTCGCCCa                  >  2:6780835/1‑93 (MQ=255)

                                                                        tttGGTGC‑GA‑‑‑GGGGGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAg                 >  1:7056299/1‑93 (MQ=255)

                                                                          tGGTGC‑GA‑‑‑GGGGGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTg               <  1:6476836/93‑1 (MQ=255)

                                                                           ggTGC‑GA‑‑‑GGGGGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAg                 <  1:6015750/90‑1 (MQ=255)

                                                                             tGC‑GA‑‑‑GGGGGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGa            <  1:6355873/93‑1 (MQ=255)

                                                                             ggC‑GA‑‑‑GGGGGGGGCCTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGa            <  2:2523193/92‑1 (MQ=255)

                                                                                 gA‑‑‑GGGGGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCa         >  2:5584242/1‑93 (MQ=255)

                                                                                     gggggggggGCTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCaa        <  2:4155415/92‑1 (MQ=255)

                                                                                     gggggggggGCTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCaa        <  2:2672177/92‑1 (MQ=255)

                                                                                        ggggggTCTGGACCCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCTCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAatat     <  2:5761026/93‑1 (MQ=17)

                                                                                        ggggggGCTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCg                               >  2:730891/1‑67 (MQ=25)

                                                                                         ggggggCTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATc    >  2:4985273/1‑93 (MQ=255)

                                                                                           ggggCTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCaa  <  2:5860552/93‑1 (MQ=255)

CACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGC‑GA‑‑‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAA  >  NC_000913/4603695‑4603874

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 16 ≤` `ATCG/ATCG` `< 24 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

AACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTA‑CTTTTTTTGATATCGCCTCGTTTGGTGC‑GA‑‑GGG‑GGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTG  >  NC_000913/4603693‑4603878

AACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTA‑CTTTTTTTGATATCGCCTCGTTTGGTGC‑GA‑‑GGG‑GGGGGGC                                                                                              <  SRR4302543.787288/94‑1 (MQ=60)

  CACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTA‑CTTTTTTTGATATCGCCTCGTTTGGTGC‑GA‑‑GGG‑GGGGGGCTT                                                                                            >  SRR4302543.2835955/1‑94 (MQ=60)

  CACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTA‑CTTTTTTTGATATCGCCTCGTTTAGTGC‑GA‑‑GGG‑GGGGG‑CTTG                                                                                           >  SRR4302543.1812907/1‑94 (MQ=60)

   ACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTA‑CTTTTTTTGATATCGCCTCGTTTGGTGC‑GA‑‑GGG‑GGGGGGCTTG                                                                                           >  SRR4302543.121752/1‑94 (MQ=60)

     CTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTA‑CTTTTTTTGATATCGCCTCGTTTGGTGC‑GA‑‑GGG‑GGGGGGCTTGAA                                                                                         >  SRR4302543.7374063/1‑94 (MQ=60)

       GAATCTACCGCCTCTACCCACTCCGCCCCCTTCGCACAGTCATCTTA‑CTTTTTTTCATTTCGCCCCGTTTGGTGA‑GAG‑GGG‑GGGGGGCTTGAAC                                                                                        <  SRR4302543.582051/94‑1 (MQ=32)

          GCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTA‑CTTTTTTTGATATCGCCTCGTTTGGTGC‑GA‑‑GGG‑GGGGGGCTTGAACCCCC                                                                                    <  SRR4302543.3377535/94‑1 (MQ=60)

           CTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTA‑CTTTTTTTGATATCGCCTCGTTTGGTGC‑GA‑‑GGG‑GGGGG‑CTTGAACCCCCAC                                                                                  >  SRR4302543.7740654/1‑94 (MQ=60)

            TAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTA‑CTTTTTTTGATATCGCCTCGTTTGGTGC‑GA‑‑GGG‑GGGGGGCTTGAACCCCCAC                                                                                  <  SRR4302543.535311/94‑1 (MQ=60)

             AGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTA‑CTTTTTTTGATATCGCCTCGTTTGGTGC‑GAG‑GGG‑GGGGGGCTTGAACCCCCAC                                                                                  <  SRR4302543.1202220/94‑1 (MQ=60)

             AGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTA‑CTTTTTTTGATATCGCCTCGTTTGGTGC‑GA‑‑GGG‑GGGGGGCTTGAACCCCCACG                                                                                 >  SRR4302543.3024666/1‑94 (MQ=60)

              GCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTA‑CTTTTTTTGATATCGCCTCGTTTGGTGC‑GA‑‑GGG‑GGGGGGCTTGAACCCCCACGT                                                                                >  SRR4302543.5607565/1‑94 (MQ=60)

               CGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTA‑CTTTTTTTGATATCGCCTCGTTTGGTGC‑GA‑‑GGG‑GGGGGGCTTGAACCCCCACGTC                                                                               >  SRR4302543.3953439/1‑94 (MQ=60)

                 CGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTA‑CTTTTTTTGATATCGCCTCGTTTGGTGC‑GA‑‑GGG‑GGGGGGCTTGAACCCCCACGTCCG                                                                             <  SRR4302543.2336128/94‑1 (MQ=60)

                  GTCTACCAATTCCGCCACCTTCGCACAGTCATCTTA‑CTTTTTTTGATATCGCCTCGTTTGGTGC‑GAGGGGG‑GGGGGGCTTGAACCCCCACGTCC                                                                              >  SRR4302543.7368345/1‑94 (MQ=60)

                  GTCTACCAATTCCGCCACCTTCGCACAGTCATCTTA‑CTTTTTTTGATATCGCCTCGTTTGGTGC‑GAG‑GGG‑GGGGGGCTTGAACCCCCACGTCCG                                                                             >  SRR4302543.4189662/1‑94 (MQ=60)

                  GTCTACCAATTCCGCCACCTTCGCACAGTCATCTTA‑CTTTTTTTGATATCGCCTCGTTTGGTGC‑GA‑‑GGG‑GGGGGGCTTGAACCCCCACGTCCGA                                                                            >  SRR4302543.2368578/1‑94 (MQ=60)

                   TCTACCAATTCCGCCACCTTCGCACAGTCATCTTA‑CTTTTTTTGATATCGCCTCGTTTGGTGC‑GAG‑GGG‑GGGGGGCTTAAACCCCCACGTCCGA                                                                            >  SRR4302543.2832051/1‑94 (MQ=60)

                   TCTACCAATTCCGCCACCTTCGCACAGTCATCTTA‑CTTTTTTTGATATCGCCTCGTTTGGTGC‑GA‑‑GGG‑GGGGGGCTTGAAACCCCACGTCCGTA                                                                           >  SRR4302543.4973720/1‑94 (MQ=60)

                    CTCCCAATGCCGCCCCCTTCGCACAGTCATCTTT‑CTTTTTTTGATATCGCCTTGTTTGGGGCGGG‑‑GGG‑GGGGGGCTTGAACCCCCACGTCCGTA                                                                           <  SRR4302543.736033/94‑1 (MQ=42)

                    CTACCAATTCCGCCACCTTCGCACAGTCATCTTA‑CTTTTTTTGATATCGCCTCGTTTGGTGC‑GAG‑GGG‑GGGGGGCTTGAACCCCCAGGTCGGTA                                                                           >  SRR4302543.268162/1‑94 (MQ=60)

                    CTACCAATTCCGCCACCTTCGCACAGTCATCTTA‑CTTTTTTTGATATCGCCTCGTTTGGTGC‑GAG‑GGG‑GGGGGGCTTGAACCCCCACGTCCGTA                                                                           >  SRR4302543.6416226/1‑94 (MQ=60)

                    CTACCAATTCCGCCACCTTCGCACAGTCATCTTA‑CTTTTTTTGATATCGCCTCGTTTGGTGC‑GA‑‑GGG‑GGGGGGCTTGAACCCCCACGTCCGTAA                                                                          >  SRR4302543.5440670/1‑94 (MQ=60)

                     TACCAATTCCGCCACCTTCGCACAGTCATCTTA‑CTTTTTTTGATATCGCCTCGTTTGGTGG‑GAG‑GGG‑GGGGGGCTTGAACCCCCACGTCCGTAA                                                                          <  SRR4302543.4096416/94‑1 (MQ=60)

                       CCAATTCCGCCACCTTCGCACAGTCATCTTA‑CTTTTTTTGATATCGCCTCGTTTGGTGC‑GA‑‑GGG‑GGGGGGCTTGAACCCCCACGTCCGTAAGAA                                                                       >  SRR4302543.4570025/1‑94 (MQ=60)

                        CAATTCCGCCACCTTCGCACAGTCATCTTA‑CTTTTTTTGATATCGCCTCGTTTGGTGC‑GA‑‑GGG‑GGGGGGCTTGAACCCCCACGTCCGTAAGAAC                                                                      >  SRR4302543.5102679/1‑94 (MQ=60)

                         AATTCCGCCCCCTTCGCACAGTCATCTTA‑CTTTTTTTGATATCGCCTCGTTTGGTGC‑GAG‑GGG‑GGGGGGCTTGAACCCCCACGTCCGTAAGAAC                                                                      <  SRR4302543.2334138/94‑1 (MQ=60)

                         AATTCCGCCACCTTCGCACAGTCATCTTA‑CTTTTTTTGATATCGCCTCGTTTGGTGC‑GA‑‑GGG‑GGGGGGCTTGACCCCCCACGTCCGTAAGAACA                                                                     <  SRR4302543.1161583/94‑1 (MQ=60)

                         AATTCCGCCACCTTCGCACAGTCATCTTA‑CTTTTTTTGATATCGCCTCGTTTGGTGC‑GA‑‑GGG‑GGGGGGCTTGAACCCCCACGTCCGTAAGAACA                                                                     <  SRR4302543.2586338/94‑1 (MQ=60)

                          ATTCCGCCACCTTCGCACAGTCATCTTA‑CTTTTTTTGATATCGCCTCGTTTGGTGC‑GAG‑GGG‑GGGGGGCTTGAACCCCCACGTCCGTAAGAcaa                                                                     >  SRR4302543.1266555/1‑91 (MQ=60)

                          ATTCCGCCACCTTCGCACAGTCATCTTA‑CTTTTTTTGATATCGCCTCGTTTGGTGC‑GAG‑GGG‑GGGGGGCTTGAACCCCCACGTCCGTAAGAACA                                                                     <  SRR4302543.483015/94‑1 (MQ=60)

                          ATTCCGCCACCTTCGCACAGTCATCTTA‑CTTTTTTTGATATCGCCTCGTTTGGTGC‑GAG‑GGG‑GGGGGGCTTGAACCCCCACGTCCGTAAGAACA                                                                     >  SRR4302543.7918708/1‑94 (MQ=60)

                           TTCCGCCACCTTCGCACAGTCATCTTA‑CTTTTTTTGATATCGCCTCGTTTGGTGC‑GAG‑GGG‑GGGGGGCTTGAACCCCCACGTCCGTAAGAACAC                                                                    >  SRR4302543.2503330/1‑94 (MQ=60)

                            tcccccccctttcgcacagtctttttcttttttttaatatctcctgtTTTGGGGC‑GA‑‑GGG‑GGGGGGCTTGAACCCCCACGTCCGTAAGAACACT                                                                   <  SRR4302543.5666247/47‑1 (MQ=14)

                            TCCGCCACCTTCGCACAGTCATCTTA‑CTTTTTTTGATATCGCCTCGTTTGGTGC‑GAGGGGG‑GGGGGGCTTGAACCCCCACGTCCGTAAGAACAC                                                                    <  SRR4302543.1036799/94‑1 (MQ=60)

                             CCGCCACCTTCGCACAGTCATCTTA‑CTTTTTTTGATATCGCCTCGTTTGGTGC‑GA‑‑GGG‑GGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAA                                                                 >  SRR4302543.1414029/1‑94 (MQ=60)

                              CGCCACCTTCGCACAGTCATCTTA‑CTTTTTTTGATATCGCCTCGTTTGGTGC‑GA‑‑GGG‑GGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAAC                                                                <  SRR4302543.3350771/94‑1 (MQ=60)

                               ccctCCTTCTCACAGTCATCTTT‑CTTTTTTTGATAGCGCCTGGTTTGGTGC‑AAG‑GGG‑GGGGGGCTTGACCCCCCACGTCCGTAAGAACACTAAC                                                                <  SRR4302543.6614276/90‑1 (MQ=50)

                               GCCCCCTTCGCACAGTCATCTTA‑CTTTTTTTGATATCGCCTCGTTTGGTGC‑GA‑‑GGG‑GGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAACA                                                               <  SRR4302543.327261/94‑1 (MQ=60)

                                CCACCTTCGCCCAGTCATCTTA‑CTTTTTTTGATATCGCCTCGTTTGGTGG‑GAGGGGG‑GGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAAC                                                                <  SRR4302543.1108939/94‑1 (MQ=60)

                                CCACCTTCGCACAGTCATCTTA‑CTTTTTTTGATATCGCCTCGTTTGGTGC‑GA‑‑GGG‑GGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAACAC                                                              >  SRR4302543.128796/1‑94 (MQ=60)

                                CCACCTTCACACAGTCATCTTA‑CTTTTGTTGATATCGCCTCGTTTGGTGC‑GAG‑GGG‑GGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAACA                                                               <  SRR4302543.625368/94‑1 (MQ=60)

                                 CACCTTCGCACAGTTATCTTA‑CTTTTGTTGATATCGCCTCGTTTGGTGC‑GA‑‑GGG‑GGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAACACC                                                             <  SRR4302543.6599084/94‑1 (MQ=60)

                                 CACCTTCGCACAGTCATCTTA‑CTTTTTTTGATATCGCCTCGTTTGGTGC‑GAG‑GGG‑GGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAACAC                                                              <  SRR4302543.7822405/94‑1 (MQ=60)

                                  ACCTTCGCACAGTCATCTTA‑CTTTTTTTGATATCGCCTCGTTTGGTGC‑GAG‑GGG‑GGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAACACC                                                             >  SRR4302543.7944717/1‑94 (MQ=60)

                                  ACCTTCGCACAGTCATCTTA‑CTTTTTTTGATATCGCCTCGTTTGGTGC‑GAG‑GGG‑GGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAACACC                                                             >  SRR4302543.950721/1‑94 (MQ=60)

                                  ACCTTCGCACAGTCATCTTA‑CTTTTTTTGATATCGCCTCGTTTGGTGC‑GAG‑GGG‑GGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAACACC                                                             >  SRR4302543.3411520/1‑94 (MQ=60)

                                  ACCTTCGCACAGTCATCTTA‑CTTTTTTTGATATCGCCTCGTTTGGTGC‑GA‑‑GGG‑GGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAACACCT                                                            >  SRR4302543.1085616/1‑94 (MQ=60)

                                   CCTTCGCACAGTCATCTTA‑CTTTTTTTGATATCGCCTCGTTTGGTGC‑GAG‑GGG‑GGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAACACCT                                                            >  SRR4302543.5556958/1‑94 (MQ=60)

                                   CCTTCGCACAGTCATCTTA‑CTTTTTTTGATATCGCCTCGTTTGGTGC‑GAG‑GGG‑GGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAACACCT                                                            >  SRR4302543.1266072/1‑94 (MQ=60)

                                    CTTCGCACAGTCATCTTA‑CTTTTTTTGATATCGCCTCGTTTGGTGC‑GAGGGGG‑GGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAACACCT                                                            >  SRR4302543.5038133/1‑94 (MQ=60)

                                    CTTCGCACAGTCATCTTA‑CTTTTTTTGATATCGCCTCGTTTGGTGC‑GAG‑GGG‑GGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAACACCTA                                                           >  SRR4302543.5012095/1‑94 (MQ=60)

                                     TTCGCAAAGTAATTTTA‑CTTTTTTTGATATCGCCTCGTTTGGTGC‑GA‑‑GGG‑GGGGGGCTTGACCCCCCACGTCCGTAAGAACACTAACACCTGAA                                                         <  SRR4302543.3105772/94‑1 (MQ=60)

                                      cttcccccggtcttttttttttttttgtttccccccggttgggggcggggGGG‑GGGGGGCTTGACCCCCCACGTCCGTAAGAACACTAACACCT                                                            <  SRR4302543.6077053/44‑1 (MQ=60)

                                      TCGCACAGTCATCTTA‑CTTTTTTTGATATCGCCTCGTTTGGTGC‑GA‑‑GGG‑GGGGGGCTTGACCCCCCACGTCCGTAAGAACACTAACACCTGAAG                                                        <  SRR4302543.1543091/94‑1 (MQ=60)

                                       CGCACCGTCATCTTA‑CTTTTTTTGATATCGCCTCGTTTGGTGC‑GA‑‑GGG‑GGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGC                                                       <  SRR4302543.1699236/94‑1 (MQ=60)

                                        GCACAGTCATCTTA‑CTTTTTTTGATATCGCCTCGTTTGGTGC‑GAG‑GGG‑GGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGC                                                       >  SRR4302543.827657/1‑94 (MQ=60)

                                        GCACAGTCATCTTA‑CTTTTTTTGATATCGCCTCGTTTGGTGC‑GA‑‑GGG‑GGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCT                                                      <  SRR4302543.203861/94‑1 (MQ=60)

                                         CACAGTCATCTTA‑TTTTTTTTGATATCGCCTCGTTTGGGGC‑GAG‑GGG‑GGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCT                                                      <  SRR4302543.6361270/94‑1 (MQ=60)

                                         CACAGTCATCTTA‑CTTTTTTTGATATCGCCTCGTTTGGTGC‑GA‑‑GGG‑GGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTA                                                     >  SRR4302543.7212505/1‑94 (MQ=60)

                                          ACAGTCATCTTATTTTTTTTTGATATCGCCTCGTTTGGTGC‑GAG‑GGG‑GGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCT                                                      <  SRR4302543.7868238/94‑1 (MQ=56)

                                           CAGTCCTCTTA‑CTTTATTTGATATCCCCTCGTTTGGTGC‑GA‑‑GGG‑GGGGGGCTTGACCCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGC                                                   <  SRR4302543.7100718/94‑1 (MQ=49)

                                           CAGTCATCTTA‑CTTTTTTTGATATCGCCTCGTTTGGTGC‑GAG‑GGG‑GGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAG                                                    <  SRR4302543.586276/94‑1 (MQ=60)

                                           CAGTCATCTTA‑CTTTTTTTGATATCGCCTCGTTTGGTGC‑GA‑‑GGG‑GGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGC                                                   <  SRR4302543.5041652/94‑1 (MQ=60)

                                            AGTCATCTTA‑CTTTTTTTGATATCGCCTCGTTTGGTGC‑GAG‑GGG‑GGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGC                                                   >  SRR4302543.3276229/1‑94 (MQ=60)

                                            AGTCATCTTA‑CTTTTTTTGATATCGCCTCGTTTGGTGC‑GAG‑GGG‑GGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGC                                                   >  SRR4302543.7836207/1‑94 (MQ=60)

                                            AGTCATCTTA‑CTTTTTTTGATATCGCCTCGTTTGGTGC‑GA‑‑GGG‑GGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCG                                                  <  SRR4302543.7994864/94‑1 (MQ=60)

                                            AGTCATCTTA‑CTTTTTTTGATATCGCCTCGTTTGGTGC‑GA‑‑GGG‑GGGGG‑CTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGC                                                 >  SRR4302543.3932899/1‑94 (MQ=60)

                                             GTCATCTTA‑CTTTTTTTGATATCGCCTCGTTTGGTGC‑GAGGGGG‑GGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGC                                                   >  SRR4302543.7920892/1‑94 (MQ=60)

                                             GTCATCTTA‑CTTTTTTTGATATCGCCTCGTTTGGTGC‑GAG‑GGG‑GGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCG                                                  <  SRR4302543.732051/94‑1 (MQ=60)

                                              TCATCTTA‑TTTTTTTTGATATCGCCT‑GTTTTGTGCGGA‑‑GGGTGGGGGGCTTGACCCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGC                                                 <  SRR4302543.3179693/94‑1 (MQ=25)

                                              TCATCTTA‑CTTTTTTTGATATCGCCTCGTTTGTTGC‑GAG‑GGG‑GGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGC                                                 <  SRR4302543.3179694/94‑1 (MQ=60)

                                              TCATCTTA‑CTTTTTTTGATATCGCCTCGTTTGGTGC‑GAG‑GGG‑GGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAACTAGCcg                                                 >  SRR4302543.3362734/1‑92 (MQ=60)

                                               agttatttttttttttttgataccgcttttttgggggcgggGGG‑GGGGGGCTTGACCCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGC                                                   <  SRR4302543.5814921/53‑1 (MQ=9)

                                               CATCTTA‑CTTTTTTTGATATCGCCTCGTTTGGTGC‑GA‑‑GGG‑GGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGT                                               >  SRR4302543.4778762/1‑94 (MQ=60)

                                                ATCTTA‑CTTTTTTTGATATCGCCTCGTTTGGTGC‑GA‑‑GGG‑GGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTC                                              >  SRR4302543.2853573/1‑94 (MQ=60)

                                                  CTTA‑CTTTTTTTGATATCGCCTCGTTTGGTGC‑GAG‑GGG‑GGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCT                                             >  SRR4302543.1113042/1‑94 (MQ=60)

                                                  CTTA‑CTTTTTTTGATATCGCCTCGTTTGGTGC‑GA‑‑GGG‑GGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTA                                            >  SRR4302543.3094124/1‑94 (MQ=60)

                                                   TTT‑CTTTTTTTGATTTCGCCTCGTTTGGTGC‑GA‑‑GGG‑GGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTAC                                           <  SRR4302543.1676844/94‑1 (MQ=52)

                                                   TTA‑CTTTTTTTGATATCGCCTCGTTTGGTGC‑GA‑‑GGG‑GGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTAC                                           <  SRR4302543.1676845/94‑1 (MQ=60)

                                                   TTA‑CTTTTTTTGATATCGCCTCGTTTGGTGC‑GA‑‑GGG‑GGGGG‑CTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACC                                          >  SRR4302543.4749509/1‑94 (MQ=60)

                                                    TA‑CTTTTTTTGATATCGCCTCGTTTGGTGC‑GA‑‑GGG‑GGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACC                                          <  SRR4302543.3332579/94‑1 (MQ=60)

                                                    TA‑CTTTTTTTGATATCGCCTCGTTTGGTGC‑GA‑‑GGG‑GGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACC                                          >  SRR4302543.6186735/1‑94 (MQ=60)

                                                       CTTTTTTTGATATCGCCTCGTTTGGTGC‑GA‑‑GGG‑GGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAA                                        <  SRR4302543.7315209/94‑1 (MQ=60)

                                                         TTTTTTGATATCGCCTCGTTTGGTGC‑GAG‑GGG‑GGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAAT                                       >  SRR4302543.6357912/1‑94 (MQ=54)

                                                           tttttgaagttcaccgagtttggtgggagGGG‑GGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAAT                                       <  SRR4302543.4908010/65‑1 (MQ=9)

                                                           TTTTTATAGCGCCTCGTTTGGTGC‑GA‑‑GGG‑GGGGG‑CTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCG                                   <  SRR4302543.2690885/94‑1 (MQ=27)

                                                           TTTTGATATCGCCTCGTTTGGTGC‑GAG‑GGG‑GGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTC                                     <  SRR4302543.3599720/94‑1 (MQ=54)

                                                           TTTTGATATCGCCTCGTTTGGTGC‑GA‑‑GGG‑GGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCC                                    <  SRR4302543.4443095/94‑1 (MQ=58)

                                                            TTTGATGTCGCCTCGTTTGGTGG‑GA‑‑GGG‑GGGGGGCTTGACCCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCG                                   <  SRR4302543.1892344/94‑1 (MQ=33)

                                                            TTTGATATCGCCTCGTTTGGTGC‑GA‑‑GGG‑GGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCG                                   >  SRR4302543.5631467/1‑94 (MQ=53)

                                                             TTGATATCGCCTCGTTTGGTGC‑GAG‑GGG‑GGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTAGCAATTCCG                                   >  SRR4302543.5831516/1‑94 (MQ=45)

                                                              TGATATCGCCTCGTTTGGTGC‑GAG‑GGG‑GGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGC                                  >  SRR4302543.6445203/1‑94 (MQ=45)

                                                                ATATCGCCTCGTTTGGTGC‑GAG‑GGG‑GGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCac                                >  SRR4302543.1960629/1‑92 (MQ=40)

                                                                ATATCGCCTCGTTTGGTGC‑GA‑‑GGG‑GGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCAC                               >  SRR4302543.3540936/1‑94 (MQ=43)

                                                                 TATCGCCTCGTTTGGTGC‑GAG‑GGG‑GGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCAC                               >  SRR4302543.6812846/1‑94 (MQ=38)

                                                                 TATCGCCTCGTTTGGTGC‑GA‑‑GGG‑GGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACC                              >  SRR4302543.2556667/1‑94 (MQ=40)

                                                                  ATCGCCTCGTTTGGTGC‑GAGGGGG‑GGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCAC                               >  SRR4302543.1176662/1‑94 (MQ=35)

                                                                  ATCGCCTCGTTTGGTGC‑GAG‑GGG‑GGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACC                              >  SRR4302543.2008659/1‑94 (MQ=35)

                                                                  ATCGCCTCGTTTGGTGC‑GAG‑GGG‑GGGGGGCTTGAACCCCCACCCCCGAAAAAACACTAACCCCCGACGCTAGCGCGTCcccaaattcccccact                              >  SRR4302543.3948744/1‑78 (MQ=19)

                                                                  ATCGCCTCGTTTGGTGC‑GA‑‑GGG‑GGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCT                             >  SRR4302543.1786036/1‑94 (MQ=38)

                                                                  ATCGCCTCGTTTGGTGC‑GA‑‑GGG‑GGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCT                             >  SRR4302543.4125827/1‑94 (MQ=38)

                                                                  ATCGCCTCGTTTGGTGC‑GA‑‑GGG‑GGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCT                             >  SRR4302543.5493016/1‑94 (MQ=38)

                                                                  ATCGCCTCGTTTGGTGC‑GA‑‑GGG‑GGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCT                             <  SRR4302543.3406721/94‑1 (MQ=40)

                                                                    CGCCTCGTTTGGTGC‑GAGGGGG‑GGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCT                             >  SRR4302543.6538743/1‑94 (MQ=30)

                                                                    CGCCTCGTTTGGTGC‑GA‑‑GGG‑GGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTC                           >  SRR4302543.3471610/1‑94 (MQ=33)

                                                                    CGCCTCGTTTGGTGC‑GA‑‑GGG‑GGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTC                           <  SRR4302543.7652141/94‑1 (MQ=35)

                                                                    CGCCTCGTTTGGTGC‑GA‑‑GGG‑GGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTC                           >  SRR4302543.109869/1‑94 (MQ=33)

                                                                    CGCCTCGTTTGGTGC‑GA‑‑GGG‑GGGGG‑CTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCctcc                          >  SRR4302543.439563/1‑90 (MQ=32)

                                                                     GCCTCGTTTGGTGC‑GAG‑GGG‑GGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTC                           >  SRR4302543.5814389/1‑94 (MQ=28)

                                                                     GCCTCGTTTGGTGC‑GA‑‑GGG‑GGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCG                          <  SRR4302543.7662910/94‑1 (MQ=33)

                                                                     GCCTCGTTTGGTGC‑GA‑‑GGG‑GGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCC                          >  SRR4302543.6317836/1‑94 (MQ=30)

                                                                      CCTCGTTTGGTGC‑GA‑‑GGG‑GGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGC                         >  SRR4302543.5644958/1‑94 (MQ=25)

                                                                      CCTCGTTTGGTGC‑GA‑‑GGG‑GGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGC                         >  SRR4302543.4525499/1‑94 (MQ=25)

                                                                      CCTCGTTTGGTGC‑GA‑‑GGG‑GGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGC                         <  SRR4302543.5217713/94‑1 (MQ=25)

                                                                       CTCGTTTGGTGC‑GAG‑GGG‑GGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGC                         >  SRR4302543.4098041/1‑94 (MQ=21)

                                                                       CTCGTTTGGTGC‑GA‑‑GGG‑GGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCC                        >  SRR4302543.145277/1‑94 (MQ=25)

                                                                         CGTTTGGTGC‑GAG‑GGG‑GGGGGGGTTGAACCCCCACGTCCGTAAGAAACCTAACACCTGAAGCTAGCAGGGCTTCCAATTCCGCCACCTTTGCCC                       >  SRR4302543.2554591/1‑94 (MQ=36)

                                                                         CGTTTGGTGC‑GAG‑GGG‑GGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGGGCGTCTACCAATTCCGCCACCTTCGCCC                       >  SRR4302543.6683161/1‑94 (MQ=20)

                                                                          GTTTGGTGC‑GAGGGGG‑GGGGGGCTTGAACCCCCACGTCCGAAAGAACACTAACACCTGAAGCCAGCGCGTCTCCCAATTCCGCCACCTTCGCCC                       >  SRR4302543.2800950/1‑94 (MQ=60)

                                                                          GTTTGGTGC‑GAG‑GGG‑GGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCA                      <  SRR4302543.3803198/94‑1 (MQ=21)

                                                                          GTTTGGTGC‑GA‑‑GGG‑GGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAG                     >  SRR4302543.7124775/1‑94 (MQ=25)

                                                                           catttggtttgagGGG‑GGGGGGCTTGAACCCCCAGGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTCCCAATTCCGCCACCTTCGCCC                       <  SRR4302543.6830683/81‑1 (MQ=11)

                                                                           TTTGGTGC‑GA‑‑GGG‑GGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGT                    <  SRR4302543.6071379/94‑1 (MQ=25)

                                                                             TGGTGC‑GA‑‑GGG‑GGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGC                  <  SRR4302543.6537860/94‑1 (MQ=30)

                                                                              GGTGC‑GAG‑GGG‑GGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGC                  >  SRR4302543.1857744/1‑94 (MQ=28)

                                                                               tggggcgggGGG‑GGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTG                   <  SRR4302543.5078299/85‑1 (MQ=29)

                                                                               GTGC‑GA‑‑GGG‑GGGGG‑CTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAG               <  SRR4302543.3963625/94‑1 (MQ=37)

                                                                                TGC‑GA‑‑GGG‑GGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAG               <  SRR4302543.6415636/94‑1 (MQ=38)

                                                                                 gggggggGGG‑GGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCG                 <  SRR4302543.1840430/87‑1 (MQ=34)

                                                                                 GC‑GAG‑GGG‑GGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAG               <  SRR4302543.3692069/94‑1 (MQ=36)

                                                                                     gagGGG‑GGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCA             <  SRR4302543.6901102/91‑1 (MQ=43)

                                                                                     G‑‑GGG‑GGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAAT           <  SRR4302543.1970515/94‑1 (MQ=47)

                                                                                     A‑‑GGG‑GTGGGTCTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAAT           <  SRR4302543.3436916/94‑1 (MQ=46)

                                                                                            GGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCA       >  SRR4302543.3668810/1‑94 (MQ=58)

                                                                                                 GCTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTG  <  SRR4302543.888770/94‑1 (MQ=60)

AACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTA‑CTTTTTTTGATATCGCCTCGTTTGGTGC‑GA‑‑GGG‑GGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTG  >  NC_000913/4603693‑4603878

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 2 ≤` `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 31 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`