Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A0 F0 I1 R1 245 579.6 28867798 97.7% 28203838 96.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,158,202 +C coding (961/975 nt) ygiRQ ← Fe‑S oxidoreductase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,158,2011.C99.4% 1951.0 / 0.9 518coding (962/975 nt)ygiRQFe‑S oxidoreductase
Reads supporting (aligned to +/- strand):  ref base . (0/3);  new base C (228/287);  total (228/290)
Fisher's exact test for biased strand distribution p-value = 2.59e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 581 total aligned reads displayed.

AAAACGCTGTCCATCTCTTCGGTAGAAAGCGGAATAGCAGGCGGGTTGATCCACACATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAG‑CTGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGCGTACAGCACTTTATCGCCCTTCACTTTCTCGAAAGAAGGCAGCAACACGTAG  >  NC_000913/3158106‑3158293
                                                                                                |                                                                                            
aaaaCGCTGTCCATCTCTTCGGTAGAAAGCGGAATAGCAGGCGGGTTGATCCACACATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAg                                                                                               <  2:2779534/96‑1 (MQ=255)
 aaaCGCTGTCCATCTCTTCGGTAGAAAGCGGAATAGCAGGCGGGTTGATCCACACATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAG‑c                                                                                             >  1:6991676/1‑96 (MQ=255)
 aaaCGCTGTCCATCTCTTCGGTAGAAAGCGGAATAGCAGGCGGGTTGATCCACACATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAG‑c                                                                                             <  2:2122350/96‑1 (MQ=255)
  aaCGCTGTCCATCTCTTCGGTAGAAAGCGGAATAGCAGGCGGGTTGATCCACCCATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAG‑cc                                                                                            <  2:6156723/96‑2 (MQ=255)
  aaCGCTGTCCATCTCTTCGGTAGAAAGCGGAATAGCAGGCGGGTTGATCCACACATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAG‑cc                                                                                            >  1:6369183/1‑95 (MQ=255)
    cGCTGTCCATCTCTTCGGTAGAAAGCGGAATAGCAGGCGGGTTGATCCACACATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAG‑cctg                                                                                          >  2:14141489/1‑93 (MQ=255)
       tGTCCATCTCTTCGGTAGAAAGCGGAATAGCAGGCGGGTTGATCCACACATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAGCCTGggt                                                                                        <  2:12412277/96‑1 (MQ=255)
        gTCCAACTCTTCGGTATAAAACGCAATGGCAGTCGGGTTGATCCACCAATAACGGTCGCCTTGTTTTTGCATCAATGCGCGTGCACAGCCTGggtt                                                                                       <  1:11163503/96‑1 (MQ=255)
         tCCATCTCTTCGGTAGAAAGCGGAATAGCAGGCGGGTTGATCCACACATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAGCCTGggttg                                                                                      <  1:3179822/96‑1 (MQ=255)
           cATCTCTTCGGTAGAAAGCGGAATAGCAGGCGGGTTGATCCACACATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAGCCTGggttggt                                                                                    <  2:8837314/96‑1 (MQ=255)
            aTCTCTTCGGTAGAAAGCGGAATAGCAGGCGGGTTGATCCACACATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAGCCTGggttggtt                                                                                   <  1:11625722/96‑1 (MQ=255)
            aTCTCTTCGGTAGAAAGCGGAATAGCAGGCGGGTTGATCCACACATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAGCCTGggttggtt                                                                                   >  2:13554395/1‑96 (MQ=255)
             tctcTTCGGTAGAAAGCGGAATAGCAGGCGGGTTGATCCACACATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAGCCTGGGTTGGttt                                                                                  <  1:9689305/96‑1 (MQ=255)
              ctctTCGGTAGAAAGCGGAATAGCAGGCGGGTTGATCCACACATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAGCCTGGGTTGGTTTc                                                                                 <  2:12007802/96‑1 (MQ=255)
              ctctTCGGTAGAAAGCGGAATAGCAGGCGGGTTGATCAACACATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAGCCTGGGTTGGTTTc                                                                                 >  1:10242925/1‑96 (MQ=255)
               tctTCGGTAGAAAGCGGAATAGCAGGCGGGTTGATCCACACATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAGCCTGGGTTGGTTTCg                                                                                >  1:6802435/1‑96 (MQ=255)
                ctTCGGTAGAAAGCGGAATAGCAGGCGGGTTGATCCACACATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAGCCTGGGTTGGTTTCgt                                                                               >  1:4068625/1‑96 (MQ=255)
                 ttCGGTAGAAAGCGGCATAGCAGGCGGGTTGATCCACACATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAGCCTGGGTTGGTTTCgtg                                                                              <  1:6563024/96‑1 (MQ=255)
                 ttCGGTAGAAAGCGGAATAGCAGGCGGGTTGATCCACACATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAGCCTGGGTTGGTTTCgtg                                                                              <  2:3401319/96‑1 (MQ=255)
                   cGGTAGAAAGCGGAATAGCAGGCGGGTTGATCCACACATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAGCCTGGGTTGGTTTCgtggt                                                                            <  2:13101227/96‑1 (MQ=255)
                   cGGTAGAAAGCGGAATAGCAGGCGGGTTGATCCACACATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAGCCTGGGTTGGTTTCgtggt                                                                            <  2:11333991/96‑1 (MQ=255)
                    ggTAGAAAGCGGAATAGCAGGCGGGTTGATCCACACATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAGCCTGGGTTGGTTTCgtggtg                                                                           <  1:50103/96‑1 (MQ=255)
                     gTAGAAAGCGGAATAGCAGGCGGGTTGATCCACACATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAGCCTGGGTTGGTTTCGTGGTGc                                                                          <  1:1774937/96‑1 (MQ=255)
                      tAGAAAGCGGAATAGCAGGCGGGTTGATCCACACATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAGCCTGGGTTGGTTTCGTGGTGCa                                                                         <  2:5994596/96‑1 (MQ=255)
                      tAGAAAGCGGAATAGCAGGCGGGTTGATCCACACATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAGCCTGGGTTGGTTTCGTGGTGCa                                                                         >  2:10715532/1‑96 (MQ=255)
                      tAGAAAGCGGAATAGCAGGCGGGTTGATCCACACATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAGCCTGGGCTGGTTTCGTGGTGCa                                                                         >  2:12912618/1‑96 (MQ=255)
                       aGAAAGCGGAATAGCAGGCGGGTTGATCCACACATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAGCCTGGGTTGGTTTCGTGGTGCAg                                                                        <  2:5120949/96‑1 (MQ=255)
                          aaGCGGAATAGCAGGCGGGTTGATCCACACATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAGCCTGGGTTGGTTTCGTGGTGCAGAAt                                                                     <  2:7228898/96‑1 (MQ=255)
                           aGCGGAATAGCAGGCGGGTTGATCCACACATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAGCCTGGGTTGGTTTCGTGGTGCAGAATa                                                                    <  2:3818415/96‑1 (MQ=255)
                            gCGGAATAGCAGGCGGGTTGATCCACACATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAGCCTGGGTTGGTTTCGTGGTGCAGAATAc                                                                   <  1:8382289/96‑1 (MQ=255)
                             ctgAATAGCCGGCGGGCTGATCCACACATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAGCCTGGGTTGGTTTCGTGGTGCAGAATAcg                                                                  >  1:10776390/3‑96 (MQ=255)
                              ggAATAGCAGGCGGGTTGATCCACACATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAGCCTGGGTTGGTTTCGTGGTGCAGAATAcgc                                                                 >  1:9921103/1‑96 (MQ=255)
                               gAATAGCAGGCGGGTTGATCCACACATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAGCCTGGGTTGGTTTCGTGGTGCAGAATAcgcg                                                                <  2:13300081/96‑1 (MQ=255)
                               gAATAGCAGGCGGGTTGATCCACACATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAGCCTGGGTTGGTTTCGTGGTGCAGAATAcgcg                                                                >  2:9716407/1‑96 (MQ=255)
                               gAATAGCAGGCGGGTTGATCCACACATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAGCCTGGGTTGGTTTCGTGGTGCAGAATAcgcg                                                                >  1:1353689/1‑96 (MQ=255)
                               gAATAGCAGGCGGGTTGATCCACACATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAGCCTGGGTTGGGTTCGTGGTGCAGAATAcgcg                                                                >  1:12404560/1‑96 (MQ=255)
                                 aTAGCAGGCGGGTTGATCCACACATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAGCCTGGGTTGGTTTCGTGGTGCAGAATACGCGaa                                                              <  2:13975932/96‑1 (MQ=255)
                                  tAGCAGGCGGGTTGATCCACACATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAGCCTGGGTTGGTTTCGTGGTGCAGAATACGCGAAg                                                             >  1:588811/1‑96 (MQ=255)
                                  tAGCAGGCGGGTTGATCCACACATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAGCCTGGGTTGGTTTCGTGGTGCAGAATACGCGAAg                                                             >  2:14216213/1‑96 (MQ=255)
                                    gCAGGCGGGTTGATCCACACATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAGCCTGGGTTGGTTTCGTGGTGCAGGATACGCGAAGCa                                                           >  1:14180638/1‑96 (MQ=255)
                                      aggaggGTTGATCCACACATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAGCCTGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATg                                                         <  2:3961868/96‑1 (MQ=255)
                                       ggCGGGTTGATCCACACATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAGCCTGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATgg                                                        >  1:7846650/1‑96 (MQ=255)
                                        gCGGGTTGATCCACACATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAGCCTGGGTTGGCTTCGTGGTGCAGAATACGCGAAGCATggg                                                       >  1:7451317/1‑96 (MQ=255)
                                         caggTTGATCCACACATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAGCCTGGGTTGGGTTCGTGGTGCAGAATACGCGAAGCATGGGc                                                      >  1:560788/3‑96 (MQ=255)
                                         cGGGTTGATCCACACATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAGCCTGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGc                                                      <  1:14380072/96‑1 (MQ=255)
                                          gggTTGATCCACACATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAGCCTGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGCg                                                     >  2:675104/1‑96 (MQ=255)
                                          gggTTGATCCACACATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAGCCTGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGCg                                                     >  2:8429647/1‑96 (MQ=255)
                                            gTTGATCCACACATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAGCCTGGGTTGGCTTCGTGGTGCAGAATACGCGAAGCATGGGCGTa                                                   >  1:8717078/1‑96 (MQ=255)
                                             ttGATCCACACATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAGCCTGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGCGTAc                                                  <  2:4838732/96‑1 (MQ=255)
                                             ttGATCCACACATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAGCCTGGGTTGGTTTCGTGGGGCAGAATACGCGAAGCATGGGCGTAc                                                  >  2:9543457/1‑96 (MQ=255)
                                              tGATCCACACATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAGCCTGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGCGTACa                                                 >  2:4642130/1‑96 (MQ=255)
                                              tGATCCACACATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAGCCTGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGCGTACa                                                 >  2:224495/1‑96 (MQ=255)
                                                aTCCACACATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAGCCTGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGCGTACAGc                                               >  2:4233910/1‑96 (MQ=255)
                                                aTCCACACATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAGCCTGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGCGTACAGc                                               >  2:1855935/1‑96 (MQ=255)
                                                aTCCACACATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAGCCTGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGCGTACAGc                                               >  2:12094372/1‑96 (MQ=255)
                                                aTCCACACATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAGCCTGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGCGTACAGc                                               >  1:1596796/1‑96 (MQ=255)
                                                 tCCACACATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAGCCTGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGCGTACAGCa                                              >  2:8644902/1‑96 (MQ=255)
                                                 tCCACACATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAGCCTGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGCGTACAGCa                                              <  2:2494108/96‑1 (MQ=255)
                                                 tCCACACATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAGCCTGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGCGTACAGCa                                              <  2:5261357/96‑1 (MQ=255)
                                                   cacacaTAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAGCCTGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGCGTACAGCACt                                            <  1:11012502/96‑1 (MQ=255)
                                                    acacaTAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAGCCTGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGCGTACAGCACtt                                           <  1:1060705/96‑1 (MQ=255)
                                                      ccaTAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAGCCTGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGCGTACAGCACTTTa                                         <  2:6878781/95‑1 (MQ=255)
                                                      acaTAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAGCCTGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGCGTACAGCACTTTa                                         <  1:5736099/96‑1 (MQ=255)
                                                       caTAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAGCCTGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGCGTACAGCACTTTAt                                        >  2:1112070/1‑96 (MQ=255)
                                                         tAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAGCCTGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGCGTACAGCACTTTATCg                                      >  1:10022000/1‑96 (MQ=255)
                                                          aGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAGCCTGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGCGTACAGCACTTTATCgg                                     <  1:13874577/96‑2 (MQ=255)
                                                           gCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAGCCTGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGCGTACAGCACTTTATCGcc                                    >  2:9312808/1‑96 (MQ=255)
                                                            cGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAGCCTGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGCGTACAGCACTTTATCGccc                                   >  1:2961401/1‑96 (MQ=255)
                                                             ggTCGCCGTGTTTTTGCATCAATGCGCGGGCACAGCCTGGGTTGGTTTCGTGGTGCAGACTACGCGAAGCATGGGCGTACAGCACTTTATccccat                                  >  2:5849266/1‑94 (MQ=255)
                                                             ggTCGCCGTGTTTTTGCATCAATGCGCGGGCACAGCCTGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGCGTACAGCACTTTATCGCCCt                                  >  2:7077391/1‑96 (MQ=255)
                                                             ggTCGCCGTGTTTTTGCATCAATGCGCGGGCACAGCCTGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGCGTACAGCACTTTATCGCCCt                                  <  1:3455253/96‑1 (MQ=255)
                                                             ggTCGCCGTGTTTTTGCATCAATGCGCGGGCACAGCCTGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGCGTACAGCACTTTATCGCCCt                                  >  1:13059670/1‑96 (MQ=255)
                                                             ggTCGCCGTGTTTTTGCATCAATGCGCGGGCACAGCCTGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGCGTACAGCACTTTATCGCCCt                                  >  2:11732249/1‑96 (MQ=255)
                                                             ggTCGCCGTGTTTTTGCATCAATGCGCGGGCACAGCCTGGGTTGGTTTCGTCGTGCAGAATAAGCGAAGCATGGGCGTACAGCACTTTATCGCCCt                                  >  1:7172581/1‑96 (MQ=255)
                                                             ggTCGCCGTGTTTTTGCATCAATGCGCGGGCACAGCCTGGGGTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGCGTACAGCACTTTATCGCCCt                                  >  2:3052249/1‑96 (MQ=255)
                                                              gTCGCCGTGTTTTTGCATCAATGCGCGGGCACAGCCTGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGCGTACAGCACTTTATCGCCCtt                                 >  1:61009/1‑96 (MQ=255)
                                                               tCGCCGTGTTTTTGCATCAATGCGCGGGCACAGCCTGGGTTGGTTTCCTGGTGCAGAATACGCGAAGCATGGGCGTACAGCACTTTATCGCCCTTc                                <  2:12762529/96‑1 (MQ=255)
                                                                cGCCGTGTTTTTGCATCAATGCGCGGGCACAGCCTGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGCGTACAGCACTTTATCGCCCTTCa                               <  1:1952016/96‑1 (MQ=255)
                                                                      gtTTTTGCATCAATGCGCGGGCACAGCCTGCGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGCGTACAGCACTTTATCGCCCTTCACTTtct                         <  1:12735557/96‑1 (MQ=255)
                                                                       tttttGCATCAATGCGCGGGCACAGCCTGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGCGTACAGCACTTTATCGCCCTTCACTTtctc                        <  1:11945331/96‑1 (MQ=255)
                                                                        ttgtGCATCAATGCGCGGGCACAGCCTGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGCGTACAGCACTTTATCGCCCTTCACTTTCTCg                       <  2:9092268/93‑1 (MQ=255)
                                                                          ttGCATCAATGCGCGGGCACAGCCTGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGCGTACAGCACTTTATCGCCCTTCACTTTCTCGaa                     >  2:7988903/1‑96 (MQ=255)
                                                                           tGCATCAATGCGCGGGCACAGCCTGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGCGTACAGCACTTTATCGCCCTTCACTTTCTCGaaa                    >  1:2376518/1‑96 (MQ=255)
                                                                           tGCATCAATGCGCGGGCACAGCCTGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGCGTACAGCACTTTATCGCCCTTCACTTTCTCGaaa                    <  2:1665312/96‑1 (MQ=255)
                                                                            gCATCAATGCGCGGTCACAGCCTGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCTTGGGCGTACAGCACTTTATCGTCCTTCACTTTCTCGAaag                   >  1:4839099/1‑96 (MQ=255)
                                                                            gCATCAATGCGCGGGCACAGCCTGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGCGTACAGCACTTTATCGCCCTTCACTTTCTCGAaag                   >  1:8909732/1‑96 (MQ=255)
                                                                             cATCAATGCGCGGGCACAGCCTGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGCGTACAGCACTTTATCGCCCTTCACTTTCTCGAaaga                  <  1:3741815/96‑1 (MQ=255)
                                                                             cATCAATGCGCGGGCACAGCCTGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGCGTACAGCACTTTATCGCCCTTCACTTTCTCGAaaga                  >  1:7315909/1‑96 (MQ=255)
                                                                             cATCAATGCGCGGGCACAGCCTGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGCGTACAGCACTTTATAGCCCTTCACTTTCTCGAaaga                  >  2:10545823/1‑96 (MQ=255)
                                                                                 aaTGCGCGGGCACAGCCTGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGCGTACAGCACTTTATCGCCCTTCACTTTCTCGAAAGAAGgc              <  1:4388767/96‑1 (MQ=255)
                                                                                  aTGCGCGGGCACAGCCTGGGTTGGTTTCGTGGTGCAGAATACGCGAAACATGGGCGTACAGCACTTTATCGCCCTTCACTTTCTCGAAAGAAGgca             <  2:10287554/96‑1 (MQ=255)
                                                                                   tGCGCGGGCACAGCCTGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGCGTACAGCACTTTATCGCCCTTCACTTTCTCGAAAGAAGgcag            <  1:52837/96‑1 (MQ=255)
                                                                                      gcgGGCACAGCCTGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGCGTACAGCACTTTATCGCCCTTCACTTTCTCGAAAGAAGGCAGCaa         >  1:9315430/1‑96 (MQ=255)
                                                                                      gcgGGCACAGCCTGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGCGTACAGCACTTTATCGCCCTTCACTTTCTCGAAAGAAGGCAGCaa         <  2:8195102/96‑1 (MQ=255)
                                                                                       cgGGCACAGCCTGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGCGTACAGCACTTTATCGCCCTTCACTTTCTCGAAAGAAGGCAGCAac        >  2:7534774/1‑96 (MQ=255)
                                                                                        gggCACAGCCTGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGCGTACAGCACTTTATCGCCCTTCACTTTCTCGAAAGAAGGCAGCAaca       >  1:2672172/1‑96 (MQ=255)
                                                                                        gggCACAGCCTGGGTTGGTTTCGTGGGGCAGAATACGCGAAGCATGGGCGTACAGCACTTTATCGCCCTTCACTTTCTCGAAAGAAGGCAGCAaca       >  2:149856/1‑96 (MQ=255)
                                                                                         ggCACAGCCTGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGCGTACAGCACTTTATCGCCCTTCACTTTCTCGAAAGAAGGCAGCAacac      <  2:5557324/96‑1 (MQ=255)
                                                                                          gCACAGCCTGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGCGTACAGCACTTTATCGCCCTTCACTTTCTCGAAAGAAGGCAGCAACACg     <  1:421440/96‑1 (MQ=255)
                                                                                           cacaGCCTGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGCGTACAGCACTTTATCGCCCTTCACTTTCTCGAAAGAAGGCAGCAACACGt    >  2:10093846/1‑96 (MQ=255)
                                                                                                |                                                                                            
AAAACGCTGTCCATCTCTTCGGTAGAAAGCGGAATAGCAGGCGGGTTGATCCACACATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAG‑CTGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGCGTACAGCACTTTATCGCCCTTCACTTTCTCGAAAGAAGGCAGCAACACGTAG  >  NC_000913/3158106‑3158293

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output Only 200 of 340 total aligned reads displayed.
CGCAAAAACGCTGTCCATCTCTTCGGTAGAAAGCGGAATAGCAGGCGGGTTGATCCACACATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACA‑G‑CTGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGCGTACAGCACTTTATCGCCCTTCACTTTCTCGAAAGAAGGCAGCAACACGTAGGTTTTTTCC  >  NC_000913/3158102‑3158302
                                                                                                      |                                                                                                    
CGCAAAAACGCTGTCCATCTCTTCGGTAGAAAGCGGAATAGCAGGCGGGTTGATCCACACATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACA‑G‑C                                                                                                      <  SRR4302518.1119474/101‑1 (MQ=60)
 GCAAAAACGCTGTCCATCTCTTCGGTAGAAAGCGGAATAGCAGGCGGGTTGATCCACACATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACA‑G‑CC                                                                                                     <  SRR4302518.6768109/101‑1 (MQ=60)
  NNNAAACGCTGTCCATCTCTTCGGTAGAAAGCGGAATAGCAGGCGGGTTGATCCACACATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACA‑G‑Cct                                                                                                    <  SRR4302518.11432352/101‑3 (MQ=60)
  NNNAAACGCTGTCCATCTCTTCGGTAGAAAGCGGAATAGCAGGCGGGTTGATCCACACATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACA‑G‑Cct                                                                                                    <  SRR4302518.9178797/101‑3 (MQ=60)
  CGAAAACGCTGTCCATCTCTTCGGTAGAAAGCGGAATAGCAGGCGGGTTGATCCACACATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACA‑G‑NNN                                                                                                    >  SRR4302518.9656618/1‑101 (MQ=60)
  CAAAAACGCTGTCCATCTCTTCGGTAGAAAGCGGAATAGCAGGCGGGTTGATCCACACATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACA‑G‑NNN                                                                                                    >  SRR4302518.8600309/1‑101 (MQ=60)
  CAAAAACGCTGTCCATCTCTTCGGTAGAAAGCGGAATAGCAGGCGGGTTGATCCACACATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACA‑G‑NNN                                                                                                    >  SRR4302518.11281592/1‑101 (MQ=60)
  CAAAAACGCTGTCCATCTCTTCGGTAGAAAGCGGAATAGCAGGCGGGTTGATCCACACATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACA‑G‑Cct                                                                                                    <  SRR4302518.555881/101‑3 (MQ=60)
   AAAAACGCTGTCCATCTCTTCGGTAGAAAGCGGAATAGCAGGCGGGTTGATCCACACATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACA‑G‑Cctg                                                                                                   <  SRR4302518.4983243/101‑4 (MQ=60)
   AAAAACGCTGTCCATCTCTTCGGTAGAAAGCGGAATAGCAGGCGGGTTGATCCACACATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACA‑G‑Cctg                                                                                                   <  SRR4302518.441950/101‑4 (MQ=60)
   AAAAACGCTGTCCATCTCTTCGGTAGAAAGCGGAATAGCAGGCGGGTTGATCCACACATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACA‑G‑Cctg                                                                                                   <  SRR4302518.6944508/101‑4 (MQ=60)
    NNNACGCTGTCCATCTCTTCGGTAGAAAGCGGAATAGCAGGCGGGTTGATCCACACATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACA‑G‑Cctag                                                                                                  <  SRR4302518.12379454/101‑5 (MQ=60)
    AAAACGCTGTCCATCTCTTCGGTAGAAAGCGGAATAGCAGGCGGGTTGATCCACACATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACA‑G‑Ccnnn                                                                                                  >  SRR4302518.8626674/1‑97 (MQ=60)
     AAACGCTGTCCATCTCTTCGGTAGAAAGCGGAATAGCAGGCGGGTTGATCCACACATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACA‑GCCTGGG                                                                                                  >  SRR4302518.2500214/1‑101 (MQ=60)
     AAACGCTGTCCATCTCTTCGGTAGAAAGCGGAATAGCAGGCGGGTTGATCCACACATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACA‑GCCTGGG                                                                                                  >  SRR4302518.1360844/1‑101 (MQ=60)
      NNNGCTGTCCATCTCTTCGGTAGAAAGCGGAATAGCAGGCGGGTTGATCCACACATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACA‑GCCTGGGT                                                                                                 <  SRR4302518.7991151/101‑1 (MQ=60)
      NNNGCTGTCCATCTCTTCGGTAGAAAGCGGAATAGCAGGCGGGTTGATCCACACATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACA‑GCCTGGGT                                                                                                 <  SRR4302518.7502044/101‑1 (MQ=60)
      ATCGCTGTCCATCTCTTCGGTAGAAAGCGGAATAGCAGGCGGGTTGATCCACACATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACA‑G‑Cctgnnn                                                                                                >  SRR4302518.12430595/1‑95 (MQ=60)
      AACGCTGTCCATCTCTTCGGTAGAAAGCGGAATAGCAGGCGGGGTGATCCACACATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACA‑GCCTGGGT                                                                                                 >  SRR4302518.2667286/1‑101 (MQ=60)
       NNNCTGTCCATCTCTTCGGTAGAAAGCGGAATAGCAGGCGGGTTGATCCACACATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACA‑GCCTGGGTT                                                                                                <  SRR4302518.9123054/101‑1 (MQ=60)
       ACGCTGTCCATCTCTTCGGTAGAAAGCGGAATAGCAGGCGGGTTGCTCCACACATAGCGGTCGCCGGGTTTTTGCATCAATGCGCGGGCACA‑GCCTGGGTT                                                                                                <  SRR4302518.3756902/101‑1 (MQ=60)
       ACGCTGTCCATCTCTTCGGTAGAAAGCGGAATAGCAGGCGGGTTGATCCACACATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACA‑GCCTGGGTT                                                                                                <  SRR4302518.6442552/101‑1 (MQ=60)
       ACGCTGTCCATCTCTTCGGTAGAAAGCGGAATAGCAGGCGGGTTGATCCACACATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACA‑G‑Cctggnnn                                                                                               >  SRR4302518.7609948/1‑94 (MQ=60)
        acCTGTCCATCTCTTCGGTAGAAAGCGGAATAGCAGGCGGGTTGATCCACACATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACA‑GCCTGGGTTG                                                                                               <  SRR4302518.3328043/99‑1 (MQ=60)
        CGCTGTCCATCTCTTCGGTAGAAAGCGGAATAGCAGGCGGGTTGATCCACACATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACA‑GCCTGGGTTG                                                                                               <  SRR4302518.4613479/101‑1 (MQ=60)
        CGCTGTCCATCTCTTCGGTAGAAAGCGGAATAGCAGGCGGGTTGATCCACACATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACA‑GCCTGGGTTG                                                                                               <  SRR4302518.2358236/101‑1 (MQ=60)
        CGCTGTCCATATCTACGGAAGAAAGCGGAATAGCAGAAGGGTTGAACCACACATAGCCGTCGCCGTGTTTTTGCATCAATGCGCGGGCCCA‑GCCTGGGTTG                                                                                               <  SRR4302518.1861141/101‑1 (MQ=58)
           TGTCCATCTCTTCGGTAGAAAGCGGAATAGCAGGCGGGTTGATCCACACATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACA‑GCCTGGGTTGGTT                                                                                            >  SRR4302518.7221283/1‑101 (MQ=60)
           NNNCCATCTCTTCGGTAGAAAGCGGAATAGCAGGCGGGTTGATCCACACATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACA‑GCCTGGGTTGGTT                                                                                            <  SRR4302518.12173172/101‑1 (MQ=60)
             TCCATCTCTTCGGTAGAAAGCGGAATAGCAGGCGGGTTGATCCACACATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACA‑GCCTGGGGTGGTTTC                                                                                          >  SRR4302518.6182396/1‑101 (MQ=60)
             NNNATCTCTTCGGTAGCAAGCGGAATAGCAGGCGGGTTGATCCACGCATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCATA‑GCCTGGGTTGGTTTC                                                                                          <  SRR4302518.9750248/101‑1 (MQ=60)
              CCATCTCTTCGGTAGAAAGCGGAATAGCAGGCGGGTTGATCCACACATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACA‑GCCTGGGTTGGTTTCG                                                                                         >  SRR4302518.736482/1‑101 (MQ=60)
                CTCTCTTCGGTAGAAAGCGGCATAGCAGGCGGGTTGATCCACACATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACA‑GCCTGGGTTGGTTTCGTG                                                                                       <  SRR4302518.6900143/101‑1 (MQ=60)
                  CTCTTCGGTAGAAAGCGGAATAGCAGGCGGGTTGATCCACACATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACA‑GCCTGGGTTGGTTTCGTNNN                                                                                     >  SRR4302518.9191795/1‑101 (MQ=60)
                   TCTTCGGTAGAAAGCGGAATAGCAGGCGGGTTGATCCACACATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACA‑GCCTGGGTTGGTTTCGTGNNN                                                                                    >  SRR4302518.14836668/1‑101 (MQ=60)
                   TCTTCGGTAGAAAGCGGAATAGCAGGCGGGTTGATCCACACATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACA‑GCCTGGGTTGGTTTCGTGGTG                                                                                    >  SRR4302518.1711746/1‑101 (MQ=60)
                   TCTTCGGTAGAAAGCGGAATAGCAGGCGGGTTGATCCACACATAGCGGTCGCCGTGTTTTTGCATCAAGGCGCGGGCCGA‑GCCTGGGTTGGTTTCTTGGGG                                                                                    >  SRR4302518.6678129/1‑101 (MQ=60)
                    CTTCGGTAGAAAGCGGAATAGCAGGCGGGTTGATCCACACATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACA‑GCCTGGGTTGGTTTCGTGGTGC                                                                                   <  SRR4302518.1854437/101‑1 (MQ=60)
                    CTTCGGTAGAAAGCGGAATAGCAGGCGGGTTGATCCACACATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACA‑GCCTGGGTTGGTTTCGTGGTGC                                                                                   >  SRR4302518.4265369/1‑101 (MQ=60)
                     NNNGGTAGAAAGCGGAATAGCAGGCGGGTTGATCCACACATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACA‑GCCTGGGTTGGTTTCGTGGTGCA                                                                                  <  SRR4302518.14336449/101‑1 (MQ=60)
                      TCGGTAGAAAGCGGAATAGCAGGCGGGTTGATCCACACATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACA‑GCCTGGGTTGGTTTCGTGGTGCAG                                                                                 <  SRR4302518.2961234/101‑1 (MQ=60)
                        GGTAGAAAGCGGAATAGCAGGCGGGTTGATCCACACATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACA‑GCCTGGGTTGGTTTCGTGGTGCANNN                                                                               >  SRR4302518.11513147/1‑101 (MQ=60)
                        GGTAGAAAGCGGAATAGCAGGCGGGTTGATCCACACATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACA‑GCCTGGGTTGGTTTCGTGGTGCAGAA                                                                               <  SRR4302518.5168857/101‑1 (MQ=60)
                         GTAGAAAGCGGAATAGCAGGCGGGTTGATCCACACATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACA‑GCCTGGGTTGGTTTCGTGGTGCAGAAT                                                                              <  SRR4302518.5918052/101‑1 (MQ=60)
                           nnnaagaggtatagcaggtgtgttgatacactatTAGCGGTCGCCGTGTTATTGCATCAATGCGCGGGCACA‑GCCTGGGTTGGTTTCGTGGTGCAGAATAC                                                                            <  SRR4302518.8556778/67‑1 (MQ=60)
                           NNNAAGCGGAATAGCAGGCGGGTTGATCCACACATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACA‑GCCTGGGTTGGTTTCGTGGTGCAGAATAC                                                                            <  SRR4302518.7804456/101‑1 (MQ=60)
                           NNNAAGCGGAATAGCAGGCGGGTTGATCCACACATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACA‑GCCTGGGTTGGTTTCGTGGTGCAGAATAC                                                                            <  SRR4302518.8793577/101‑1 (MQ=60)
                           NNNAAGCGGAATAGCAGGCGGGTTGATCAACACATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACA‑GCCTGGGTTGGTTTCGTGGTGCAGAATAC                                                                            <  SRR4302518.10689554/101‑1 (MQ=60)
                             AAAGCGGAATAGCAGGCGGGTTGATCCACACATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACA‑GCCTGGGTTGGTTTCGTGGTGCAGAATACGC                                                                          <  SRR4302518.1069797/101‑1 (MQ=60)
                             AAAGCCGAATAGCATGCGGGCTGATCCACACATAGCGGTCGCCGTGATTTTGCATCAATGCGCGGGCACA‑GCCTGGGTTGGGTTCGTGGTGCAGAATACGC                                                                          >  SRR4302518.3965413/1‑101 (MQ=60)
                              AAGCGGAATAGCAGGCGGGTTGATCCACACATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACA‑GCCTGGGTTGGTTTCGTGGTGCAGAATACGCG                                                                         <  SRR4302518.61283/101‑1 (MQ=60)
                              AAGCGGAATAGCAGGCGGGTTGATCCACACATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACA‑GCCTGGGTTGGTTTCGTGGTGCAGAATACGCG                                                                         <  SRR4302518.662715/101‑1 (MQ=60)
                               AGCGGAATAGCAGGCGGGTTGATCCACACATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCGCA‑GCCTTGGTTGGTTTCGTGGTGCAGAATACGNNN                                                                        >  SRR4302518.10772915/1‑101 (MQ=60)
                                NNNGAATAGCAGGCGGGTTGATCCACACATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACA‑GCCTGGGTTGGTTTCGTGGTGCAGAATACGCGAA                                                                       <  SRR4302518.7790715/101‑1 (MQ=60)
                                GGGGAATAGCAGGCGGGTTGATCCACACATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACA‑GCCTGGGTTGGTTTCGTGGTGCAGAATACGCNNN                                                                       >  SRR4302518.14967660/1‑101 (MQ=60)
                                GCGGAATAGCAGGCGGGTTGATCCACACATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACA‑GCCTGGGTTGGTTTCGTGGTGCAGAATACGCGAA                                                                       <  SRR4302518.5310885/101‑1 (MQ=60)
                                GCGGAATAGCAGGCGGGTTGATCCACACATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACA‑GCCTGGGTTGGTTTCGTGGTGCAGAATACGCGAA                                                                       <  SRR4302518.2021460/101‑1 (MQ=60)
                                GCGGAATAGCAGGCGGGTTGATCCACACATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACA‑GCCTGGGTTGGTTTCGTGGTGCAGAATACGCGAA                                                                       <  SRR4302518.6693232/101‑1 (MQ=60)
                                GCCGAATGGCAGGCGGGTTTATCTACACAATGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACA‑GCCTGGGTTGGTTTCGTTGTGCAGAATACGaaaa                                                                       <  SRR4302518.6286080/101‑5 (MQ=60)
                                 CGGAATAGCAGGCGGGTTGCTCCACACATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACA‑GCCTGGGTTGGTTTCGTGGTGCAGAATACGCGAAG                                                                      <  SRR4302518.1005689/101‑1 (MQ=60)
                                 CGGAATAGCAGGCGGGTTGATCCACACATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACA‑GCCTGGGTTGGTTTCGTGGTGCAGAATACGCGNNN                                                                      >  SRR4302518.10387342/1‑101 (MQ=60)
                                 CGGAATAGCAGGCGGGTTGATCCACACATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACA‑GCCTGGGTTGGTTTCGTGGTGCAGAATACGCGAAG                                                                      <  SRR4302518.5595851/101‑1 (MQ=60)
                                  GGAATAGCAGGCGGGTTGATCCACACATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACA‑GCCTGGGTTGGTTTCGTGGTGCAGAATACGCGANNN                                                                     >  SRR4302518.10394033/1‑101 (MQ=60)
                                   GAATAGCAGGCGGGTTGATCCACACATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACA‑GCCTGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCA                                                                    >  SRR4302518.1409777/1‑101 (MQ=60)
                                   GAATAGCAGGCGGGTTGATCCACACATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACA‑GCCTGGGTTGGGTTCGTGGTGCAGAATACGCGAANNN                                                                    >  SRR4302518.12989201/1‑101 (MQ=60)
                                    gaaagcaggagggtttatcagCACATAGTGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACATC‑CTGGGTTGGTTTCGTGCTGCAGAATACGCGAATCAT                                                                   <  SRR4302518.1161675/80‑1 (MQ=60)
                                    GATAGCAGGCGGGTTGATCCACACATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACA‑GCCTGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCAT                                                                   <  SRR4302518.3662363/101‑1 (MQ=60)
                                      TAGCAGGCGGGTTGATCCACACATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACA‑GCCTGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGG                                                                 >  SRR4302518.611699/1‑101 (MQ=60)
                                      NNNCAGGCGGGTTGATCCACACATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACA‑GCCTGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGG                                                                 <  SRR4302518.10245309/101‑1 (MQ=60)
                                       NNNAGGCGGGTTGATCCACACATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACA‑GCCTGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGG                                                                <  SRR4302518.12709384/101‑1 (MQ=60)
                                        NNNGGCGGGTTGATCCACACATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACA‑GCCTGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGC                                                               <  SRR4302518.15046679/101‑1 (MQ=60)
                                        GCAGGCGGGTTGATCCACACATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACA‑GCCTGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGC                                                               <  SRR4302518.3815552/101‑1 (MQ=60)
                                         CAGGCGGGTTGATCCACACATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACA‑GCCTGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGNNN                                                              >  SRR4302518.14635096/1‑101 (MQ=60)
                                           NNNGGGTTGATCCACACATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACA‑GCCTGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGCGTA                                                            <  SRR4302518.11091109/101‑1 (MQ=60)
                                           GGCGGGTTGATCCACACATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACA‑GCCTGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGCNNN                                                            >  SRR4302518.8241428/1‑101 (MQ=60)
                                           GGCGGGTTGATCCACACATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACA‑GCCTGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGCGTA                                                            <  SRR4302518.2207797/101‑1 (MQ=60)
                                            GCGGGTTGATCCACACATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACA‑GCCTGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGCGTAC                                                           >  SRR4302518.2729091/1‑101 (MQ=60)
                                            GCGGGTTGATCCACACATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACA‑GCCTGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGCGNNN                                                           >  SRR4302518.12196363/1‑101 (MQ=60)
                                            GCGGGTTGATCCACACATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACA‑GCCTGGGTTGGTTTCGTGGTGCAGAAAACGCGAAGCATGGGCGTAC                                                           <  SRR4302518.1759820/101‑1 (MQ=60)
                                            GCGGGTTGATCCACACATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACA‑GCCTGGGTTGGCTTCGTGGTGCAGAATACGCGAAGCATGGGCGNNN                                                           >  SRR4302518.7835739/1‑101 (MQ=60)
                                             CGGGTTGATCCACACATAGCGGTCGCCGTGTTTTTGCATCAATGTGCGGGCACA‑GCCTGGGTTGGGTTCGTGGTGCAGAGTACGCGAAGCATGGGCGTNNN                                                          >  SRR4302518.13615610/1‑101 (MQ=60)
                                             CGGGTTGATCCACACATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACA‑GCCTGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGCGTACA                                                          >  SRR4302518.688835/1‑101 (MQ=60)
                                             CGGGGTGATCCACACATAGCGGTCGCCGGGTTTTTGCATCAATGCGCGGGCACA‑GCCCGGGTTGGTTTCGGGGTGCAGAATACGCGAAGCAgcggggtaga                                                          >  SRR4302518.3343298/1‑91 (MQ=60)
                                             CAGGTTGATCCACACATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACA‑GCCTGGGTTGGGTTCGTGGTGCAGAATACGCGAAGCATGGGCGTACA                                                          >  SRR4302518.582604/1‑101 (MQ=60)
                                              GGGTTGATCCACACATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACA‑GCCTGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGCGTANNN                                                         >  SRR4302518.7641851/1‑101 (MQ=60)
                                              GGGTTGATCCACACATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACA‑GCCTGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGCGTANNN                                                         >  SRR4302518.9434258/1‑101 (MQ=60)
                                              GGGTTGATCCACACATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACA‑GCCTGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGCGTACAG                                                         >  SRR4302518.4042490/1‑101 (MQ=60)
                                              GGGTTGATCCACACATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACA‑GCCTGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGCGTACAG                                                         >  SRR4302518.280648/1‑101 (MQ=60)
                                               GGTTGATCCACACATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACA‑GCCTGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGCGTACAGC                                                        <  SRR4302518.5735361/101‑1 (MQ=60)
                                               GGTTGATCCACACATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACA‑GCCTGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGCGTACAGC                                                        >  SRR4302518.428021/1‑101 (MQ=60)
                                               GGTTGATCCACACATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACA‑GCCTGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGCGTACAGC                                                        <  SRR4302518.3203332/101‑1 (MQ=60)
                                                NNNGATCCACACATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACA‑GCCTGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGCGTACAGCA                                                       <  SRR4302518.13265954/101‑1 (MQ=60)
                                                GTTGATCCCCACAACGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCCCA‑GCCTAGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGCGTACAGCA                                                       <  SRR4302518.4339349/101‑1 (MQ=60)
                                                 TTGATCCACACATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACA‑GCCTGGGATGGTTTCGTGGTGCAGAATACGCGAAGCATGGGCGCACAGNNN                                                      >  SRR4302518.8920304/1‑101 (MQ=60)
                                                  TGATCCGCACATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACA‑GCCGGGGTTGGTTTCGTGGTGCAGAATACGAGAAGCATGGGCGGACAGCNNN                                                     >  SRR4302518.11579080/1‑101 (MQ=60)
                                                  NNNTCCCCACATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACA‑GCCTGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGCGTACAGCATT                                                     <  SRR4302518.11231680/101‑1 (MQ=60)
                                                   GATCCACACATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACA‑GCCTGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGCGTACAGCACTT                                                    <  SRR4302518.1102464/101‑1 (MQ=60)
                                                    ATCCACACATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACA‑GCCTGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGCGTACAGCACTTT                                                   >  SRR4302518.1666494/1‑101 (MQ=60)
                                                     TCCACACATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACA‑GCCTGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGCGTACAGCACTNNN                                                  >  SRR4302518.12600578/1‑101 (MQ=60)
                                                     TACACACATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACA‑GCCTGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGCGTACAGCACTTTA                                                  >  SRR4302518.3619552/1‑101 (MQ=60)
                                                      NNNCACATCGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACA‑GCCTGGGTTGGTTTCGTGGTGCAGAATACGCGAACCATGGGCGTACAGCACTTTAT                                                 <  SRR4302518.11679474/101‑1 (MQ=60)
                                                      CCACACATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACA‑GCCTGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGCGTACAGCACTTTAT                                                 <  SRR4302518.5197976/101‑1 (MQ=60)
                                                       CACACATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACA‑GCCTGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGCGTACAGCACTTTATC                                                <  SRR4302518.1966594/101‑1 (MQ=60)
                                                        NNNCATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACA‑GCCTGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGCGTACAGCACTTTATCG                                               <  SRR4302518.7817874/101‑1 (MQ=60)
                                                        NNNCATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACA‑GCCTGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGCGTACAGCACTTTATCG                                               <  SRR4302518.13066159/101‑1 (MQ=60)
                                                        ACACATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACA‑GCCTGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGCGTACAGCACTTTANNN                                               >  SRR4302518.9939993/1‑101 (MQ=60)
                                                         NNNATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACA‑GCCTGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGCGTACAGCACTTTATCGG                                              <  SRR4302518.14509588/101‑1 (MQ=60)
                                                         CACATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACA‑GCCTGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGCGTACAGCACTTTATNNN                                              >  SRR4302518.13704004/1‑101 (MQ=60)
                                                          ACATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACA‑GCCTGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGCGTACAGCACTTTATCGAC                                             >  SRR4302518.419677/1‑101 (MQ=60)
                                                            NNNGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACA‑GCCTGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGCGTACAGCACTTTATCGCCCT                                           <  SRR4302518.15081204/101‑1 (MQ=60)
                                                            NNNGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACA‑GCCTGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGCGTACAGCACTTTATCGCCCT                                           <  SRR4302518.14877984/101‑1 (MQ=60)
                                                            NNNGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACA‑GCCTGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGCGTACAGCACTTTATCGCCCC                                           <  SRR4302518.9260365/101‑1 (MQ=60)
                                                            ATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACA‑GCCTGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGCGTACAGCACTTTATCGCCCT                                           <  SRR4302518.3616079/101‑1 (MQ=60)
                                                             tggtGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACA‑GCCTGGGTTGGTTTCGTGGTGCAGAATACGGGAATCATGGGCGTACAGCATTTGATCGCCNNN                                          >  SRR4302518.10861772/5‑101 (MQ=60)
                                                             TAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACA‑GCCTGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGCGTACAGCACTTTATCGCCNNN                                          >  SRR4302518.13546837/1‑101 (MQ=60)
                                                             TAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACA‑GCCTGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGCGTACAGCACTTTATCGCCNNN                                          >  SRR4302518.10498031/1‑101 (MQ=60)
                                                             TAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACA‑GCCTGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGCGTACAGCACTTTATCGCCCTT                                          <  SRR4302518.5250445/101‑1 (MQ=60)
                                                              cgcggcaGCCGTGTTTTTGCATCCATGCGCGGGCACA‑GCCTGGGTTGGTTTCGTGGTGCAGAATACGCGAAGAATGGGCGTACAGCACTTTATCGCCCTTC                                         <  SRR4302518.2172339/94‑1 (MQ=60)
                                                               NNNGTCGCAGTGTTTTTGCATCAATGCGCGGGCACA‑GCCTGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGCGTACAGCACTTTATCGCCCTTCA                                        <  SRR4302518.13992112/101‑1 (MQ=60)
                                                               GCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACA‑GCCTGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGCGTACAGCACTTTATCGCCCTTCA                                        <  SRR4302518.2041078/101‑1 (MQ=60)
                                                                CGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACA‑GCCTGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGCGTACAGCACTTTATCGCCCTTCAC                                       >  SRR4302518.3100382/1‑101 (MQ=60)
                                                                CGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACA‑GCCTGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGCGTACAGCACGTTATCGTCCTTCAC                                       >  SRR4302518.2824180/1‑101 (MQ=60)
                                                                 GGTCGCCGTGTTTTTGCATCAATGCGCGGGCACA‑GCCTGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGCGTACAGCACTTTATCGCCCTTCNNN                                      >  SRR4302518.9376104/1‑101 (MQ=60)
                                                                 GGTCGCCGTGTTTTTGCATCAATGCGCGGGCACA‑GCCTGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGCGTACAGCACTTTATCGCCCTTCACT                                      <  SRR4302518.474829/101‑1 (MQ=60)
                                                                 GGTCGCCGTGTTTTTGCATCAATGCGCGGGCACA‑GCCTGGGTTGGTTTCGTCGTGCAGAATAAGCGAAGCATGGGCGTACAGCACTTTATCGCCCTTCNNN                                      >  SRR4302518.7549228/1‑101 (MQ=60)
                                                                  nnngcaGTGTTTTTGCATTAATGTGCGGGCACACC‑CTGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGCGTACAGCACTTTATCGCCCTTCACTT                                     <  SRR4302518.13269249/95‑1 (MQ=60)
                                                                  GTCGCCGTGTTTTTGCATCAATGCGCGGGCACA‑GCCTGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGCGTACAGCACTTTATCGCCCTTCANNN                                     >  SRR4302518.10310567/1‑101 (MQ=60)
                                                                  GTCGCCGTGTTTTTGCATCAATGCGCGGGCACA‑GCCTGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGCGGACAGCACTTTATCGCCCTTCACTT                                     >  SRR4302518.63428/1‑101 (MQ=60)
                                                                   NNNCCGTGTTTTTGCATCAATGCGCGGGCACA‑GCCTGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGCGTACAGCACTTTATCGCCCTTCACTTT                                    <  SRR4302518.10249128/101‑1 (MQ=60)
                                                                   NNNCCGTGTTTTTGCATCAATGCGCGGGCACA‑GCCTGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGCGTACAGCACTTTATCGCCCTTCACTTT                                    <  SRR4302518.8386462/101‑1 (MQ=60)
                                                                   NNNCCGTGTTTTTGCATCAATGCGCGGGCACA‑GCCTGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGCGTACAGCACTTTATCGCCCTTCACTAT                                    <  SRR4302518.11608599/101‑1 (MQ=60)
                                                                    NNNCGTGTTTTTGCATCAATGCGCGGGCACA‑GCCTGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGCGTACAGCACTTTATCGCCCTTCACTTTC                                   <  SRR4302518.8470908/101‑1 (MQ=60)
                                                                     NNNGTGTTTTTGCATCAATGCGCGGGCACA‑GCCTGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGCGTACAGCACTTTATCGCCCTTCACTTTCT                                  <  SRR4302518.7559308/101‑1 (MQ=60)
                                                                     GCCGTGTTTTTGCATCAATGCGCGGGCACA‑GCCTGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGCTTACAGCACTTTATCGCCCTTCACTTNNN                                  >  SRR4302518.10678791/1‑101 (MQ=60)
                                                                     GCCGTGTTTTTGCATCAATGCGCGGGCACA‑GCCTGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGCGTACAGCACTTTATCGCCCTTCACTTTCT                                  <  SRR4302518.3744885/101‑1 (MQ=60)
                                                                     GCCGTGTTTTTGCATCAATGCGCGGGCACA‑GCCTGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGCGTACAGCACTTTATCGCCCTTCACTTTCT                                  >  SRR4302518.3149885/1‑101 (MQ=60)
                                                                      NNNTGTTTTTGCATCAATGCGCGGGCACA‑GCCTGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGCGTACAGCACTTTATCGCCCTTCACTTTCTC                                 <  SRR4302518.12514742/101‑1 (MQ=60)
                                                                      CCGTGTTTTTGCATCAATGCGCGGGCACA‑GCCTGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGCGTACAGCACTTTATCGCCCTTCACTTTCTC                                 >  SRR4302518.6712211/1‑101 (MQ=60)
                                                                      CCGTGTTTTTGCATCAATGCGCGGGCACA‑GCCTGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGCGTACAGCACTTTATCGCCCTTCACTTTCTC                                 <  SRR4302518.4428371/101‑1 (MQ=60)
                                                                       NNNGTTTTTGCATCAATGCGCGGGCACA‑GCCTGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGCGTACAGCACTTTATCGCCCTTCACTTTCTCG                                <  SRR4302518.14597889/101‑1 (MQ=60)
                                                                       CGTGTTTTTGCATCAATGCGCGGGCACA‑GCCTGGGTTGGCTTCGAGGTGCAGATTACGCGAAGCATGGGCGTACAGAACTTTATCGCCCTGCACTTTCTCG                                >  SRR4302518.6234306/1‑101 (MQ=60)
                                                                        NNNTTTTTGCATCAATGCGCGGGCACA‑GCCTGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGCGTACAGCCCTTTATCGCCCTTCACTTTCTCGA                               <  SRR4302518.14806790/101‑1 (MQ=60)
                                                                        NNNTTTTTGCATCAATGCGCGGGCACA‑GCCTGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGCGTACAGCACTTTATCGCCCTTCACTTTCTCGA                               <  SRR4302518.7465040/101‑1 (MQ=60)
                                                                        NNNTTTTTGCATCAATGCGCGGGCACA‑GCCTGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGCGTACAGCACTTTATCGCCCTTCACTTTCTCCA                               <  SRR4302518.11650035/101‑1 (MQ=60)
                                                                        GTGTTTTTGCATCAATGCGCGGGCACA‑GCCTGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGCGTACAGCACTTTATCGCCCTTCACTTTCTCGA                               >  SRR4302518.4492495/1‑101 (MQ=60)
                                                                        GTGTTTTTGCATCAATGCGCGGGCACA‑GCCTGGGGTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGCGTACAGCACTTTATCGCCCTTCACTTTCTCGA                               >  SRR4302518.5400410/1‑101 (MQ=60)
                                                                         TGTTTTTGGAACAATGCGCGGGCACA‑GCCTGGGTTGGTTTCGTGGTGCAGAATACCCGAAGCATGGGCGTACAGCACTTTATCGCCCTTCACTTTCTCGAA                              <  SRR4302518.3250283/101‑1 (MQ=60)
                                                                         TGTTTTTGCATCAATGCGCGGGCACA‑GCCTGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGCGTACAGCACTTTATCGCCCTTCACTTTCTCGAA                              >  SRR4302518.6894078/1‑101 (MQ=60)
                                                                          GTTTTTGCATCAATGCGCGGGCACA‑GCCTGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGCGTACAGCACTTTATCGCCCTTCACTTTCTCGNNN                             >  SRR4302518.12558307/1‑101 (MQ=60)
                                                                          GTTTTTACATCAATGCGCGGGCACA‑GCCTGTGTTTGTTTCGTGGTGCAGAATACGCGAAGCATGGGCGTACAGCACTTTATCGCCCTTCACTTTCTCGAAA                             <  SRR4302518.69603/101‑1 (MQ=60)
                                                                           NNNTTGCATCAATGCGCGGGCACA‑GCCTGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGCGTACAGCACTTTATCGCCCTTCACTTTCTCGAACG                            <  SRR4302518.11175830/101‑1 (MQ=60)
                                                                            TTTTGCATCAATGCGCGGGCACA‑GCCTGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGCGTACAGCACTTTATCGCCCTTCACTTTCTCGAAAGA                           <  SRR4302518.4012278/101‑1 (MQ=60)
                                                                            NNNTGCATCAATGCGCGGGCACA‑GCCTGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGCGTACAGCACTTTATCGCCCTTCCCTTTCTCGAAAGC                           <  SRR4302518.14499699/101‑1 (MQ=60)
                                                                            NNNTGCATCAATGCGCGGGCACA‑GCCTGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGCGTACAGCACTTTATCGCCCTTCACTTTCTCGAAAGG                           <  SRR4302518.11827542/101‑1 (MQ=60)
                                                                             TTTGCATCAATGCGCGGGCACA‑GCCTGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGCGTACAGCACTTTATCGCCCTTCACTTTCTCGAAAGAA                          >  SRR4302518.150405/1‑101 (MQ=60)
                                                                             TTTGCATCAATGCGCGGGCACA‑GCCTGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGCGTACAGCACTTTATCGCCCTTCACTTTCTCGAAAGAA                          <  SRR4302518.3442129/101‑1 (MQ=60)
                                                                             TTTGCATCAATGCGCGGGAACA‑GCCTGGGTTGGTTTGGTGGTGCAGAATACGCGAAGCATGGGCGTACAGCACTTTATCGCCCTTCACTTTCTCGAAAGAA                          <  SRR4302518.6392007/101‑1 (MQ=60)
                                                                              TTGCATCAATGCGCGGGCACA‑GCCTGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGCGTACAGCACTTTATCGCCCTTCACTTTCTCGAAAGAAG                         <  SRR4302518.6005683/101‑1 (MQ=60)
                                                                               TGCATCAATGCGCGGGCACA‑GCCTGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGCGTACAGCACTTTATCGCCCTTCACTTTCTCGAAAGAAGC                        >  SRR4302518.2491833/1‑101 (MQ=60)
                                                                               TGCATCAATGCGCGGGCACA‑GCCTGGGATGGTTTCGGGGTGCAGAATACGCGAAGCATGATCGTACAGCACTTTATCGCCCTCCACTTTCTCGAAAtaaga                        >  SRR4302518.2741537/1‑96 (MQ=60)
                                                                                GCATCAATGCGCGGTCACA‑GCCTGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCTTGGGCGTACAGCACTTTATCGTCCTTCACTTTCTCGAAAGAAGTC                       >  SRR4302518.5092082/1‑101 (MQ=60)
                                                                                GCATCAATGCGCGGGCACA‑GCCTGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGCGTACAGCACTTTATCGCCCTTCACTTTCTCGAAAGAAGGC                       <  SRR4302518.5314331/101‑1 (MQ=60)
                                                                                GCATCAATGCGCGGGCACA‑GCCTGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGCGTACAGCACTTTATCGCCCTTCACTTTCTCGAAAGAAGGC                       <  SRR4302518.4610250/101‑1 (MQ=60)
                                                                                GCATCAATGCGCGGGCACA‑GCCTGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGCGTACAGCACTTTATCGCCCTTCACTTTCTCCAAAGAANNN                       >  SRR4302518.9341218/1‑101 (MQ=60)
                                                                                 CATCAATGCGCGGGCACA‑GCCTGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGCGTACAGCACTTTATCGCCCTTCACTTTCTCGAAAGAAGNNN                      >  SRR4302518.7458095/1‑101 (MQ=60)
                                                                                 CATCAATGCGCGGGCACA‑GCCTGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGCGTACAGCACTTTATCGCCCTTCACTTTCTCGAAAGAAGNNN                      >  SRR4302518.7696596/1‑101 (MQ=60)
                                                                                  NNNAATGCGCGGGCACA‑GCCTGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGCGTACAGCACTTTATCGCCCTTCACTTTCTCGAAAGAAGGCAG                     <  SRR4302518.9039068/101‑1 (MQ=60)
                                                                                  CTCAATGCGCGGGCACA‑GCCTGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGCGTACAGCACTTTATCGCCCTTCACTTTCTCGAAAGAAGGCAG                     <  SRR4302518.54936/101‑1 (MQ=60)
                                                                                     NNNGCGCGGGCACA‑GCCTGGGTTGGTTTCGTTGTGCAGAATACGCGAAGCATGGGCGTACAGCACTTTATCGCCCTTCACTTTCTCGAAAGAAGGCAGCAA                  <  SRR4302518.12723261/101‑1 (MQ=60)
                                                                                      ATGCGCGGGCACA‑GCCTGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGCGTACAGCACTTTATCGCCCTTCACTTTCTCGAAAGAAGGCAGCAAC                 <  SRR4302518.5977356/101‑1 (MQ=60)
                                                                                      ATGCGCGGGCACA‑GCCTGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGCGTACAGCACTTTATCGCCCTTCACTTTCTCGAAAGAAGGCAGCAAC                 >  SRR4302518.3576439/1‑101 (MQ=60)
                                                                                        NNNCGGGCACA‑GCCTGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGCGTACAGCACTTTATCGCCCTTCACTTTCTCGAAAGAAGGCAGCAACTC               <  SRR4302518.11344867/101‑1 (MQ=60)
                                                                                        NNNCGGGCACA‑GCCTGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGCGTACAGCACTTTATCGCCCTTCACTTTCTCGAAAGAAGGCAGCAACAC               <  SRR4302518.10129900/101‑1 (MQ=60)
                                                                                        GCGCGGGCACA‑GCCTGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGCGTACAGCACTTTATCGCCCTTCACTTTCTCGAAAGAAGGCAGCAACAC               <  SRR4302518.6040270/101‑1 (MQ=60)
                                                                                        GCGCGGGCACA‑GCCTGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGCGTACAGCACTTTATCGCCCTTCACTTTCTCGAAAGAAGGCAGCAACAC               <  SRR4302518.7340606/101‑1 (MQ=60)
                                                                                           CGGGCACA‑GCCTGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGCGTACAGCACTTTATCGCCCTTCACTTTCTTGAAAGAAGGCAGCAACACNNN            >  SRR4302518.13411031/1‑101 (MQ=60)
                                                                                            GGGCACAGG‑CTGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGCGTACAGCACTTTATCGCCCTTCACTTTCTCGAAAGAAGGCAGCAACACGTAG           <  SRR4302518.7258822/101‑1 (MQ=60)
                                                                                            GGGCACA‑GCCTGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGCGTACAGCCCTTTCTCGCCCTTCACTTTCTCGAAAGAAGGCAGCAACACGTAG           <  SRR4302518.4325399/101‑1 (MQ=60)
                                                                                            GGGCACA‑GCCTGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGCGTACAGCACTTTATCGCCCTTCACTTTCTCGAAAGAAGGCAGCAACACGTAG           >  SRR4302518.6967312/1‑101 (MQ=60)
                                                                                            GGGCACA‑GCCTGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGCGTACAGCACTTTATCGCCCTTCACTTTCTCGAAAGAAGGCAGCAACACGTAG           >  SRR4302518.2799691/1‑101 (MQ=60)
                                                                                            GGGCACA‑GCCTGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGCGTACAGCACTTTATCGCCCTTCACTTTCTCGAAAGAAGGCAGCAACACGNNN           >  SRR4302518.13178319/1‑101 (MQ=60)
                                                                                               CACA‑GCCTGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGCGTACAGCACTTTATCGCCCTTCACTTTCTCGAAAGAAGGCAGCAACCCGTAGGTT        >  SRR4302518.4343838/1‑101 (MQ=60)
                                                                                               CACA‑GCCTGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGCGTACAGCACTTTATCGCCCTTCACTTTCTCGAAAGAAGGCAGCAACACGTAGGTT        >  SRR4302518.1589073/1‑101 (MQ=60)
                                                                                                ACA‑GCCTGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGCGTACAGCACTTTATCGGCCTTCACTTTCTCGAAAGAAGGCAGCAACACGTAGGNNN       >  SRR4302518.13477525/1‑101 (MQ=60)
                                                                                                ACA‑GCCTGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGCGTACAGCACTTTATCGCCCTTCACTTTCTCGAAAGAAGGCAGCAACACGTAGGTTT       <  SRR4302518.2281455/101‑1 (MQ=60)
                                                                                                ACA‑GCCTGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGCGTACAGCACTTTATCGCCCTTCACTTTCTCGAAAGAAGGCAGCAACACGTAGGTTT       >  SRR4302518.5879866/1‑101 (MQ=60)
                                                                                                ACA‑GCCTGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGCGTACAGCACTTTATCGCCCTTCACTTTCTCGAAAGAAGGCAGCAACACGTAGGNNN       >  SRR4302518.7890420/1‑101 (MQ=60)
                                                                                                ACA‑GCCTGGGTTGGGTTCGTGGTGCAGAATACGCGAAGCATGGGCGTACAGCACTTTATCGCCCTTCACTTTCTCGAAAGAAGGCAGCAACACGTAGGTTT       >  SRR4302518.7031354/1‑101 (MQ=60)
                                                                                                 CA‑GCCTGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGCGTACAGCACTTTATCGCCCTTCACTTTCTCGAAAGAAGGCAGCAACACGTAGGTTTT      >  SRR4302518.1300000/1‑101 (MQ=60)
                                                                                                  nnncCTGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGCGTACAGCACTTTATCGCCCTTCACTTTCTCGAAAGAAGGCAGCAACACGTAGGTTTT      <  SRR4302518.8298386/97‑1 (MQ=60)
                                                                                                  N‑N‑NTGGGTTGGTTTCGTGGTGTAGAATACGCGAAGCATGGGCGTACAGCACTTTATCGCCCTTCACTTTCTCGAAAGAAGGCAGCAACACGTAGGTTTTTT    <  SRR4302518.13367216/101‑1 (MQ=60)
                                                                                                   agcCTGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGCGTACAGCACTTTATCGCCCTTCACTTTCTCGAAAGAAGGCAGCAACACGTAGGTTTTT     >  SRR4302518.1247169/4‑101 (MQ=60)
                                                                                                   agcCTGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGCGTACAGCACTTTATCGCCCTTCACTTTCTCGAAAGAAGGCAGCAACACGTAGGTTNNN     >  SRR4302518.7851713/4‑101 (MQ=60)
                                                                                                   agcCTGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGCGGACAGAACTTTATCGCCCTTCACTTTCTCGAAAGAAGGCAGAAACCCGTAGGTTTTT     >  SRR4302518.7099138/4‑101 (MQ=60)
                                                                                                    C‑CTGGGTTGGTTTGGTGGTGCAGAATACGCGAAGCATGGGCGTACAGCACTTTATCGCCCTTCACTTTCTCGAAAGAAGGCAGCAACACGTAGGTTTTNNN   >  SRR4302518.14481596/1‑101 (MQ=60)
                                                                                                    C‑CTGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGCGTACAGCACTTTATCGCCCTTCACTTTCTCGAAAGAAGGCAGCAACACGTAGGTTTTNNN   >  SRR4302518.10029601/1‑101 (MQ=60)
                                                                                                    C‑CTGGGTTGGGTTCGTGGTGCAGAATACGCGAAGCATGGGCGTACAGCACTTTATCGCCCTTCACTTTCTCGAAAGAAGGCAGCAACACGTAGGTTTTNNN   >  SRR4302518.11712103/1‑101 (MQ=60)
                                                                                                      NNNGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGCGTACAGCACTTTATCGCCCTTCACTTTCTCGAAAGAAGGCAGCAACACGTAGGTTTTTTCC  <  SRR4302518.9774816/101‑1 (MQ=60)
                                                                                                      CTGTGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGCGTACAGCACTTTATCGCCCTTCACTTTCTCGAAAGAAGGCAGCAACACGTAGGTTTTTTCC  <  SRR4302518.2369808/101‑1 (MQ=60)
                                                                                                      CTGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGCGTACAGCACTTTATCGCCCTTCACTTTCTCGAAAGAAGGCAGCAACACGTAGGTTTTTTCC  >  SRR4302518.2168307/1‑101 (MQ=60)
                                                                                                      |                                                                                                    
CGCAAAAACGCTGTCCATCTCTTCGGTAGAAAGCGGAATAGCAGGCGGGTTGATCCACACATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACA‑G‑CTGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGCGTACAGCACTTTATCGCCCTTCACTTTCTCGAAAGAAGGCAGCAACACGTAGGTTTTTTCC  >  NC_000913/3158102‑3158302

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑