Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 1873 183.1 9376148 98.2% 9207377 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,158,202 +C coding (961/975 nt) ygiRQ ← Fe‑S oxidoreductase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,158,2011.C100.0% 536.4 / NA 172A321G (GCT→GGT) ygiRQFe‑S oxidoreductase
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base C (66/106);  total (66/106)

Only 100 of 198 total aligned reads displayed.

ACGCTGTCCATCTCTTCGGTAGAAAGCGGAATAGCAGGCGGGTTGATCCACACATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAG‑CTGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGCGTACAGCACTTTATCGCCCTTCACTTTCTCGAAAGAAGGCAGCAACA  >  NC_000913/3158109‑3158288
                                                                                             |                                                                                       
aCGCTGTCCATCTCTTCGGTAGAAAGCGGAATAGCAGGCGGGTTGATCCACACATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAg                                                                                          >  1:2433893/1‑93 (MQ=255)
aCGCTGTCCATCTCTTCGGTAGAAAGCGGAATAGCAGGCGGGTTGATCCACACATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAg                                                                                          <  2:4016148/93‑1 (MQ=255)
  gCTGTCCATCTCTTCGGTAGAAAGCGGAATAGCAGGCGGGTTGATCCACACATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAG‑cc                                                                                       >  1:2990125/1‑92 (MQ=255)
   cTGTCCATCTCTTCGGTAGAAAGCGGAATAGCAGGCGGGTTGATCCACACATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAG‑cct                                                                                      >  1:1058830/1‑91 (MQ=255)
    tGTCCATCTCTTCGGTAGAAAGCGGAATAGCAGGCGGGTTGATCCACACATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAG‑cctg                                                                                     <  1:2904732/93‑4 (MQ=255)
    tGTCCATCTCTTCGGTAGAAAGCGGAATAGCAGGCGGGTTGATCCACACATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAG‑cctg                                                                                     <  1:3456818/93‑4 (MQ=255)
     gTCCATCTCTTCGGTAGAAAGCGGAATAGCAGGCGGGTTGATCCACACATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAG‑cctgg                                                                                    <  2:942746/93‑5 (MQ=255)
     gTCCATCTCTTCGGTAGAAAGCGGAATAGCAGGCGGGTTGATCCACACATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAG‑cctgg                                                                                    >  1:3253842/1‑89 (MQ=255)
     gTCCATCTCTTCGGTAGAAAGCGGAATAGCAGGCGGGTTGATCCACACATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAG‑cctgg                                                                                    <  2:3672680/93‑5 (MQ=255)
     gTCCATCTCTTCGGTAGAAAGCGGAATAGCAGGCGGGTTGATCCACACATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAG‑cctgg                                                                                    <  2:4319799/93‑5 (MQ=255)
        cATCTCTTCGGTAGAAAGCGGAATAGCAGGCGGGTTGATCCACACATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAGCCTGggtt                                                                                  <  2:1063259/93‑1 (MQ=255)
        cATCTCTTCGGTAGAAAGCGGAATAGCAGGCGGGTTGATCCACACATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAGCCTGggtt                                                                                  >  2:747463/1‑93 (MQ=255)
         aTCTCTTCGGTAGAAAGCGGAATAGCAGGCGGGTTGATCCACACATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAGCCTGggttg                                                                                 <  2:1613028/93‑1 (MQ=255)
          tctcTTCGGTAGAAAGCGGAATAGCAGGCGGGTTGATCCACACATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAGCCTGggttgg                                                                                <  1:1800276/93‑1 (MQ=255)
          tctcTTCGGTAGAAAGCGGAATAGCAGGCGGGTTGATCCACACATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAGCCTGggttgg                                                                                <  1:1153771/93‑1 (MQ=255)
           ctctTCGGTAGAAAGCGGAATAGCAGGCGGGTTGATCCACACATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAGCCTGggttggt                                                                               <  1:4403223/93‑1 (MQ=255)
            tctTCGGTAGAAAGCGGAATAGCAGGCGGGTTGATCCACACATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAGCCTGggttggtt                                                                              >  1:4490014/1‑93 (MQ=255)
             ctTCGGTAGAAAGCGGAATAGCAGGCGGGTTGATCCACACATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAGCCTGGGTTGGttt                                                                             >  2:2625601/1‑93 (MQ=255)
             ctTCGGTAGAAAGCGGAATAGCAGGCGGGTTGATCCACACATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAGCCTGGGTTGGttt                                                                             >  1:4622834/1‑93 (MQ=255)
                cGGTAGAAAGCGGAATAGCAGGCGGGTTGATCCACACATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAGCCTGGGTTGGTTTCgt                                                                          <  1:810587/93‑1 (MQ=255)
                  gTAGAAAGCGGAATAGCAGGCGGGTTGATCCACACATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAGCCTGGGTTGGTTTCgtgg                                                                        >  1:2808366/1‑93 (MQ=255)
                  gTAGAAAGCGGAATAGCAGGCGGGTTGATCCACACATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAGCCTGGGTTGGTTTCgtgg                                                                        >  1:2077275/1‑93 (MQ=255)
                   tAGAAAGCGGAATAGCAGGCGGGTTGATCCACACATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAGCCTGGGTTGGTTTCgtggt                                                                       <  2:137694/93‑1 (MQ=255)
                     gAAAGCGGAATAGCAGGCGGGTTGATCCACACATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAGCCTGGGTTGGTTTCGTGGTGc                                                                     >  2:2368889/1‑93 (MQ=255)
                     gAAAGCGGAATAGCAGGCGGGTTGATCCACACATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAGCCTGGGTTGGTTTCGTGGTGc                                                                     <  2:3099176/93‑1 (MQ=255)
                     gAAAGCGGAATAGCAGGCGGGTTGATCCACACATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAGCCTGGGTTGGTTTCGTGGTGc                                                                     <  1:788306/93‑1 (MQ=255)
                     gAAAGCGGAATAGCAGGCGCGTTGATCCACACATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAGCCTGGGTTGGTTTCGTGGTGc                                                                     >  2:4573051/1‑93 (MQ=255)
                       aaGCGGAATAGCAGGCGGGTTGATCCACACATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAGCCTGGGTTGGTTTCGTGGTGCAg                                                                   >  2:4182021/1‑93 (MQ=255)
                         gCGGAATAGCAGGCGGGTTGATCCACACATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAGCCTGGGTTGGTTTCGTGGTGCAGaa                                                                 <  2:3771742/93‑1 (MQ=255)
                         gCGGAATAGCAGGCGGGTTGATCCACACATAGCGGTCGCCGTGCTTTTGCATCAATGCGCGGGCACAGCCTGGGTTGGTTTCGTGGTGCAGaa                                                                 <  1:2684307/93‑1 (MQ=255)
                          cGGAATAGCAGGCGGGTTGATCCACACATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAGCCTGGGTTGGTTTCGTGGTGCAGAAt                                                                <  1:522778/93‑1 (MQ=255)
                           ggAATAGCAGGCGGGTTGATCCACACATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAGCCTGGGTTGGTTTCGTGGTGCAGAATa                                                               <  2:4447414/93‑1 (MQ=255)
                            gAATAGCAGGCGGGTTGATCCACACATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAGCCTGGGTTGGTTTCGTGGTGCAGAATAc                                                              <  2:412055/93‑1 (MQ=255)
                            gAATAGCAGGCGGGTTGATCCACACATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAGCCTGGGTTGGTTTCGTGGTGCAGAATAc                                                              >  2:208623/1‑93 (MQ=255)
                            gAATAGCAGGCGGGTTGATCCACACATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAGCCTGGGTTGGTTTCGTGGTGCAGAATAc                                                              <  2:1290175/93‑1 (MQ=255)
                            gAATAGCAGGCGGGTTGATCCACACATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAGCCTGGGTTGGTTTCGTGGTGCAGAATAc                                                              <  2:1200793/93‑1 (MQ=255)
                            gAATAGCAGGCGGGTTGATCCACACATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAGCCTGGGTTGGTTTCGTGGTGCAGAATAc                                                              <  1:3607655/93‑1 (MQ=255)
                             aaTAGCAGGCGGGTTGATCCACACATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAGCCTGGGTTGGTTTCGTGGTGCAGAATAcg                                                             <  1:4264569/93‑1 (MQ=255)
                                aGCAGGCGGGTTGATCCACACATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAGCCTGGGCTGGTTTCGTGGTGCAGAATACGCGa                                                          >  2:1371844/1‑93 (MQ=255)
                                   aGGCGGGTTGATCCACACATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAGCCTGGGTTGGTTTCGTGGTGCAGAATACGCGAAGc                                                       >  2:1737868/1‑93 (MQ=255)
                                    ggCGGGTTGATCCACACATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAGCCTGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCa                                                      >  1:982492/1‑93 (MQ=255)
                                     gCGGGTTGATCCACACATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAGCCTGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCAt                                                     <  2:1139620/93‑1 (MQ=255)
                                     gCGGGTTGATCCACACATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAGCCTGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCAt                                                     <  1:4452351/93‑1 (MQ=255)
                                      cGGGTTGATCCACACATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAGCCTGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATg                                                    <  2:2414503/93‑1 (MQ=255)
                                      cGGGTTGATCCACACATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAGCCTGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATg                                                    <  1:2785250/93‑1 (MQ=255)
                                       gggTTGATCCACACATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAGCCTGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATgg                                                   <  1:63790/93‑1 (MQ=255)
                                        ggTTGATCCACACATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAGCCTGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATggg                                                  <  1:380991/93‑1 (MQ=255)
                                         gTTGATCCACACATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAGCCTGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGc                                                 >  1:2856750/1‑93 (MQ=255)
                                           tGATCCACACATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAGCCTGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGCGt                                               <  2:613478/93‑1 (MQ=255)
                                            gATCCACACATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAGCCTGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGCGTa                                              <  2:2242615/93‑1 (MQ=255)
                                              tCCACACATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAGCCTGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGCGTACa                                            <  2:3923410/93‑1 (MQ=255)
                                              tCCACACATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAGCCTGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGCGTACa                                            <  1:1384723/93‑1 (MQ=255)
                                              tCCACACATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAGCCTGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGCGTACa                                            <  2:903252/93‑1 (MQ=255)
                                              tCCACACATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAGCCTGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGCGTACa                                            <  1:4307252/93‑1 (MQ=255)
                                              tCCACACATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAGCCTGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGCGTACa                                            >  2:246823/1‑93 (MQ=255)
                                                cacacaTAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAGCCTGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGCGTACAGc                                          <  1:3698425/93‑1 (MQ=255)
                                                 acacaTAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAGCCTGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGCGTACAGCa                                         >  1:4019557/1‑93 (MQ=255)
                                                  cacaTAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAGCCTGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGCGTACAGCAc                                        <  1:2338977/93‑1 (MQ=255)
                                                  cacaTAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAGCCTGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGCGTACAGCAc                                        >  1:4081561/1‑93 (MQ=255)
                                                   acaTAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAGCCTGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGCGTACAGCACt                                       <  2:3733611/93‑1 (MQ=255)
                                                    caTAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAGCCTGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGCGTACAGCACtt                                      >  1:3754156/1‑93 (MQ=255)
                                                    caTAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAGCCTGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGCGTACAGCACtt                                      <  2:292129/93‑1 (MQ=255)
                                                     aTAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAGCCTGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGCGTACAGCACttt                                     <  2:3822593/93‑1 (MQ=255)
                                                      tAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAGCCTGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGCGTACAGCACTTTa                                    <  2:662709/93‑1 (MQ=255)
                                                      tAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAGCCTGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGCGTACAGCACTTTa                                    >  2:4660291/1‑93 (MQ=255)
                                                       aGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAGCCTGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGCGTACAGCACTTTAt                                   <  1:821091/93‑1 (MQ=255)
                                                        gCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAGCCTGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGCGTACAGCACTTTATc                                  <  1:2489212/93‑1 (MQ=255)
                                                        gCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAGCCTGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGCGTACAGCACTTTATc                                  <  1:1689415/93‑1 (MQ=255)
                                                        gCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAGCCTGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGCGTACAGCACTTTATc                                  <  2:40668/93‑1 (MQ=255)
                                                        gCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAGCCTGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGCGTACAGCACTTTATc                                  >  1:1464023/1‑93 (MQ=255)
                                                         cGGGCGCCGTGTTTTTGCATCAATGCGCGGGCACAGCCTGGGTTGGTTTCGTGGTGCAGAATATGCGGAGCATGGGAGTACAGCACTTTATCg                                 >  2:1455690/1‑93 (MQ=255)
                                                           gTCGCCGTGTTTTTGCATCAATGCGCGGGCACAGCCTGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGCGTACAGCACTTTATCGcc                               <  2:4490014/93‑1 (MQ=255)
                                                            tCGCCGTGTTTTTGCATCAATGCGCGTGCACAGCCTGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGCGTACAGCACTTTATCGccc                              <  1:2941448/93‑1 (MQ=255)
                                                            tCGCCGTGTTTTTGCATCAATGCGCGGGCACAGCCTGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGCGTACAGCACTTTATCGccc                              <  2:2596308/93‑1 (MQ=255)
                                                            tCGCCGTGTTTTTGCATCAATGCGCGGGCACAGCCTGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGCGTACAGCACTTTATCGccc                              >  2:3292071/1‑93 (MQ=255)
                                                               ccGTGTTTTTGCATCAATGCGCGGGCACAGCCTGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGCGTACAGCACTTTATCGCCCTTc                           <  1:1941148/93‑1 (MQ=255)
                                                                 gtgtTTTTGCATCAATGCGCGGGCACAGCCTGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGCGTACAGCACTTTATCGCCCTTCAc                         <  1:2664973/93‑1 (MQ=255)
                                                                 gtgtTTTTGCATCAATGCGCGGGCACAGCCTGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGCGTACAGCACTTTATCGCCCTTCAc                         >  1:1616020/1‑93 (MQ=255)
                                                                 gtgtTTTTGCATCAATGCGCGGGCACAGCCTGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGCGTACAGCACTTTATCGCCCTTCAc                         <  1:897527/93‑1 (MQ=255)
                                                                  tgtTTTTGCATCAATGCGCGGGCACAGCCTGGGTTGGTTTCGAGGTGCAGAATACGCGAAGCATGGGCGTACAGCACTTTATCGCCCTTCACt                        >  1:3068534/1‑93 (MQ=255)
                                                                    tttttGCATCAATGCGCGGGCACAGCCTGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGCGTACAGCACTTTATCGCCCTTCACttt                      <  2:2851386/93‑1 (MQ=255)
                                                                    tttttGCATCAATGCGCGGGCACAGCCTGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGCGTACAGCACTTTATCGCCCTTCACttt                      <  1:416317/93‑1 (MQ=255)
                                                                        tGCATCAATGCGCGGGCACAGCCTGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGCGTACAGCACTTTATCGCCCTTCACTTTCTCg                  <  1:1608235/93‑1 (MQ=255)
                                                                         gCATCAATGCGCGGGCACAGCCTGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGCGTACAGCACTTTATCGCCCTTCACTTTCTCGa                 <  1:2181842/93‑1 (MQ=255)
                                                                           aTCAATGCGCGGGCACAGCCTGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGCGTACAGCACTTTATCGCCCTTCACTTTCTCGaaa               <  1:185721/93‑1 (MQ=255)
                                                                           aTCAATGCGCGGGCACAGCCTGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGCGTACAGCACTTTATCGCCCTTCACTTTCTCGaaa               <  1:4332960/93‑1 (MQ=255)
                                                                           aTCAATGCGCGGGCACAGCCTGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGCGTACAGCACTTTATCGCCCTTCACTTTCTCGaaa               <  1:482046/93‑1 (MQ=255)
                                                                           aTCAATGCGCGGGCACAGCCTGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGCGTACAGCACTTTATCGCCCTTCACTTTCTCGaaa               >  1:1864991/1‑93 (MQ=255)
                                                                           aTCAATGCGCGGGCACAGCCTGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGCGTACAGCACTTTATCGCCCTTCACTTTCTCGaaa               >  2:2266356/1‑93 (MQ=255)
                                                                            tCAATGGGCGGGCAAAGCCTGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGCGTACAGCACTTTATCGCCCTTCACTTTCTCGAaag              <  2:3552292/93‑1 (MQ=255)
                                                                            tCAATGCGCGGGCACAGCCTGGCTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGCGTACAGCACTTTATCGCCCTTCACTTTCTCGAaag              <  2:2964153/93‑1 (MQ=255)
                                                                              aaTGCGCGGGCACAGCCTGGGTTTGTTTCGTGGTGCAGAATACGCGAAGCATGGGCGTACAGCACTTTATCGCCCTTCACTTTCTCGAaagaa            <  1:4382251/93‑1 (MQ=255)
                                                                              aaTGCGCGGGCACAGCCTGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGCGTACAGCACTTTATCGCCCTTCACTTTCTCGAaagaa            <  1:3207661/93‑1 (MQ=255)
                                                                              aaTGCGCGGGCACAGCCTGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGCGTACAGCACTTTATCGCCCTTCACTTTCTCGAaagaa            >  1:3642743/1‑93 (MQ=255)
                                                                               aTGCGCGGGCACAGCCTGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGCGTACAGCGCTTTATCGCCCTTCACTTTCTCGAaagaag           <  2:2661098/93‑1 (MQ=255)
                                                                                tGCGCGGGCACAGCCTGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGCGTACAGCACTTTATCGCCCTTCACTTTCTCGAAAGAAgg          <  2:3626655/93‑1 (MQ=255)
                                                                                  ggcgGGCACAGCCTGGGTTGATTTCGGGGTGCATAATACGCGAAGCATGGGCGTACAGCACTTTATCGCCCTTCACTTTCTCGAAAGAAGgca        <  1:1653653/92‑1 (MQ=255)
                                                                                  cgcgGGCACAGCCTGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGCGTACAGCACTTTATCGCCCTTCACTTTCTCGAAAGAAGgca        <  2:2039636/93‑1 (MQ=255)
                                                                                  cgcgGGCACAGCCTGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGCGTACAGCACTTTATCGCCCTTCACTTTCTCGAAAGAAGgca        <  1:1142546/93‑1 (MQ=255)
                                                                                   gcgGGCACAGCCTGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGCGTACAGCACTTTATCGCCCTTCACTTTCTCGAAAGAAGgcag       <  2:3425854/93‑1 (MQ=255)
                                                                                             |                                                                                       
ACGCTGTCCATCTCTTCGGTAGAAAGCGGAATAGCAGGCGGGTTGATCCACACATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAG‑CTGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGCGTACAGCACTTTATCGCCCTTCACTTTCTCGAAAGAAGGCAGCAACA  >  NC_000913/3158109‑3158288

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CGCTGTCCATCTCTTCGGTAGAAAGCGGAATAGCAGGCGGGTTGATCCACACATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAG‑CTGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGCGTACAGCACTTTATCGCCCTTCACTTTCTCGAAAGAAGGCAGCAACACGTAGG  >  NC_000913/3158110‑3158294
                                                                                             |                                                                                            
CGCTGTCCATCTCTTCGGTAGAAAGCGGAATAGCAGGCGGGTTGATCCACACATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAG‑CC                                                                                             >  SRR4302535.3011092/1‑94 (MQ=60)
 actcTCCATCTCTTCGGTAGAAAGCGGAATAGCAGGCGGGTTGATCCACACATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAG‑Cct                                                                                            <  SRR4302535.1061734/90‑3 (MQ=60)
 GCTGTCCATCTCTTCGGTAGAAAGCGGAATAGCAGGCGGGTTGATCCACACATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAG‑Cct                                                                                            >  SRR4302535.1066371/1‑92 (MQ=60)
  CTGTCCATCTCTTCGGTAGAAAGCGGAATAGCAGGCGGGTTGATCCACACATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAG‑Cctg                                                                                           >  SRR4302535.4471262/1‑91 (MQ=60)
   TGTCCATCTCTTCGGTAGAAAGCGGAATAGCAGGCGGGTTGATCCACACATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAGCCTGG                                                                                           <  SRR4302535.2925265/94‑1 (MQ=60)
   TGTCCATCTCTTCGGTAGAAAGCGGAATAGCAGGCGGGTTGATCCACACATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAGCCTGG                                                                                           >  SRR4302535.3276449/1‑94 (MQ=60)
   TGTCCATCTCTTCGGTAGAAAGCGGAATAGCAGGCGGGTTGATCCACACATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAGCCTGG                                                                                           <  SRR4302535.3480848/94‑1 (MQ=60)
   TGTCCATCTCTTCGGTAGAAAGCGGAATAGCAGGCGGGTTGATCCACACATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAGCCTGG                                                                                           >  SRR4302535.1949676/1‑94 (MQ=60)
    GTCCATCTCTTCGGTAGAAAGCGGAATAGCAGGCGGGTTGATCCACACATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAGCCTGGG                                                                                          >  SRR4302535.2256704/1‑94 (MQ=60)
       CATCTCTTCGGTCGAAAGCGGAATAGCAGGCGGGTTGATCCACACATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAGCCTGGGTTG                                                                                       <  SRR4302535.4504140/94‑1 (MQ=60)
       CATCTCTTCGGTAGAAAGCGGAATAGCAGGCGGGTTGATCCACACATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAG‑Cctggggtg                                                                                      >  SRR4302535.4420123/1‑86 (MQ=60)
        ATCTCTTCGGTAGAAAGCGGAATAGCAGGCGGGTTGATCCACACATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAGCCTGGGTTGG                                                                                      >  SRR4302535.4399399/1‑94 (MQ=60)
         TCTCTTCGGTAGAAAGCGGAATAGCAGGCGGGTTGATCCACACATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAGCCTGGGTTGGT                                                                                     <  SRR4302535.1813290/94‑1 (MQ=60)
         TCTCTTCGGTAGAAAGCGGAATAGCAGGCGGGTTGATCCACACATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAGCCTGGGTTGGT                                                                                     <  SRR4302535.1162018/94‑1 (MQ=60)
          CTCTTCGGTAGAAAGCGGAATAGCGGGCGGGTTGATCCACACATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAGCCTGGGTTGGTT                                                                                    <  SRR4302535.888625/94‑1 (MQ=60)
          CTCTTCGGTAGAAAGCGGAATAGCAGGCGGGTTGATCCACACATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAGCCTGGGTTGGTT                                                                                    >  SRR4302535.4520834/1‑94 (MQ=60)
          CTCTTCGGTAGAAAGCGGAATAGCAGGCGGGTTGATCCACACATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAGCCTGGGTTGGTT                                                                                    <  SRR4302535.4433357/94‑1 (MQ=60)
           TCTTCGGTAGAAAGCGGAATAGCAGGCGGGTTGATCCACACATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAGCCTGGGTTGGTTT                                                                                   >  SRR4302535.4654233/1‑94 (MQ=60)
              TCGGTAGAAAGCGGAATAGCAGGCGGGTTGATCCACACATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAGCCTGGGTTGGTTTCGT                                                                                >  SRR4302535.168464/1‑94 (MQ=60)
               CGGTAGAAAGCGGAATAGCAGGCGGGTTGATCCACACATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAGCCTGGGTTGGTTTCGTG                                                                               <  SRR4302535.816254/94‑1 (MQ=60)
               CGGTAGAAAGCGGAATAGCAGGCGGGTTGATCCACACATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAGCCTGGGTTGGTTTCGTG                                                                               <  SRR4302535.3567088/94‑1 (MQ=60)
                GGTAGAAAGCGGAATAGCAGGCGGGTTGATCCACACATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAGCCTGGGTTGGTTTCGTGG                                                                              >  SRR4302535.2092240/1‑94 (MQ=60)
                GGTAGAAAGCGGAATAGCAGGCGGGTTGATCCACACATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAGCCTGGGTTGGTTTCGTGG                                                                              >  SRR4302535.1223820/1‑94 (MQ=60)
                GGTAGAAAGCGGAATAGCAGGCGGGTTGATCCACACATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAGCCTGGGTTGGTTTCGTGG                                                                              >  SRR4302535.2828332/1‑94 (MQ=60)
                 GTAGAAAGCGGAATAGCAGGCGGGTTGATCCACACATAGCGGTCGCCGTGTTTTTGCATCAATGTGCGGGCACAGCCTGGGCTGGTTTCGTGGT                                                                             >  SRR4302535.2759011/1‑94 (MQ=60)
                    GAAAGCGGAATAGCAGGCGGGTTGATCCACACATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAGCCTGGGTTGGTTTCGTGGTGCA                                                                          <  SRR4302535.793842/94‑1 (MQ=60)
                       AGCGGAATAGCAGGCGGGTTGATCCACACATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAGCCTGGGTTGGTTTCGTGGTGCAGAA                                                                       >  SRR4302535.691648/1‑94 (MQ=60)
                        GCGGAATAGCAGGCGGGTTGATCCACACATAGCGGTCGCCGTGCTTTTGCATCAATGCGCGGGCACAGCCTGGGTTGGTTTCGTGGTGCAGAAT                                                                      <  SRR4302535.2703502/94‑1 (MQ=60)
                         CGGAATAGCAGGCGGGTTGATCCACACATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAGCCTGGGTTGGTTTCGTGGTGCAGAATA                                                                     <  SRR4302535.526436/94‑1 (MQ=60)
                          GGAATAGCAGGCGGGTTGATCCACACATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAGCCTGGGTTGGTTTCGTGGTGCAGAATAC                                                                    >  SRR4302535.1875739/1‑94 (MQ=60)
                           GAATAGCAGGCGGGTTGATCCACACATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAGCCTGGGTTGGTTTCGTGGTGCAGAATACG                                                                   <  SRR4302535.3632675/94‑1 (MQ=60)
                            AATAGCAGGCGGGTTGATCCACACATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAGCCTGGGTTGGTTTCGTGGTGCAGAATACGC                                                                  <  SRR4302535.4293774/94‑1 (MQ=60)
                              TAGCAGGCGGGTTGATCCACACATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAGCCTGGGTTGGTTTCGTGGTGCAGAATACGCGA                                                                >  SRR4302535.2141646/1‑94 (MQ=60)
                              TAGCAGGCGGGTTGATCCACACATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAGCCTGGGTTGGTTTCGTGGTGCAGAATACGCGA                                                                >  SRR4302535.602614/1‑94 (MQ=60)
                               AGCAGGCGGGTTGATCCACACATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAGCCTGGGTTGGTTTCGTGGTGCAGAATACGCGAA                                                               >  SRR4302535.1398958/1‑94 (MQ=60)
                               AGCAGGCGGGTTGATCCACACATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAGCCTGGGTTGGTTTCGTGGTGCAGAATACGCGAA                                                               <  SRR4302535.2806654/94‑1 (MQ=60)
                                GCAGGCGGGTTGATCCACACATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAGCCTGGGTTGGTTTCGTGGTGCAGAATACGCGAAG                                                              <  SRR4302535.4217316/94‑1 (MQ=60)
                                  AGGCGGGTTGATCCACACATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAGCCTGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCA                                                            <  SRR4302535.4089673/94‑1 (MQ=60)
                                  AGGCGGGTTGATCCACACATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAGCCTGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCA                                                            >  SRR4302535.951807/1‑94 (MQ=60)
                                  AGGCGGGTTGATCCACACATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAGCCTGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCA                                                            >  SRR4302535.989472/1‑94 (MQ=60)
                                   GGCGGGTTGATCCACACATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAGCCTGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCAT                                                           >  SRR4302535.2013913/1‑94 (MQ=60)
                                   GGCGGGTTGATCCACACATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAGCCTGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCAT                                                           >  SRR4302535.2400715/1‑94 (MQ=60)
                                   GGCGGGTTGATCCACACATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAGCCTGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCAT                                                           >  SRR4302535.3674229/1‑94 (MQ=60)
                                    GCGGGTTGATCCACACATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAGCCTGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATG                                                          <  SRR4302535.4482886/94‑1 (MQ=60)
                                    GCGGGTTGATCCACACATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAGCCTGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATG                                                          <  SRR4302535.2497518/94‑1 (MQ=60)
                                     CGGGTTGATCCACACATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAGCCTGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGG                                                         <  SRR4302535.2805069/94‑1 (MQ=60)
                                      GGGTTGATCCACACATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAGCCTGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGG                                                        >  SRR4302535.4416343/1‑94 (MQ=60)
                                      GGGTTGATCCACACATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAGCCTGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGG                                                        <  SRR4302535.64270/94‑1 (MQ=60)
                                       GGTTGATCCACACATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAGCCTGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGC                                                       <  SRR4302535.383626/94‑1 (MQ=60)
                                       GGTTGATCCACACATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAGCCTGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGC                                                       >  SRR4302535.2876990/1‑94 (MQ=60)
                                       GGTTGATCCACACATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAGCCTGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGC                                                       <  SRR4302535.2929196/94‑1 (MQ=60)
                                        GTTGATCCACACATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAGCCTGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGCG                                                      <  SRR4302535.3644092/94‑1 (MQ=60)
                                           GATCCACACATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAGCCTGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGCGTAC                                                   <  SRR4302535.1645823/94‑1 (MQ=60)
                                            ATCCACACATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAGCCTGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGCGTACA                                                  <  SRR4302535.2925046/94‑1 (MQ=60)
                                            ATCCACACATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAGCCTGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGCGTACA                                                  >  SRR4302535.3762381/1‑94 (MQ=60)
                                             TCCACACATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAGCCTGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGCGTACAG                                                 <  SRR4302535.4336717/94‑1 (MQ=60)
                                             TCCACACATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAGCCTGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGCGTACAG                                                 <  SRR4302535.4653380/94‑1 (MQ=60)
                                             TCCACACATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAGCCTGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGCGTACAG                                                 <  SRR4302535.1394607/94‑1 (MQ=60)
                                               CACACATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAGCCTGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGCGTACAGCA                                               >  SRR4302535.4047061/1‑94 (MQ=60)
                                               CACACATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAGCCTGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGCGTACAGCA                                               <  SRR4302535.3724042/94‑1 (MQ=60)
                                                GCACATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAGCCTGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGCGTACAGCAC                                              <  SRR4302535.1625054/94‑1 (MQ=60)
                                                ACACATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAGCCTGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGCGTACAGCAC                                              >  SRR4302535.4109479/1‑94 (MQ=60)
                                                ACACATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAGCCTGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGCGTACAGCAC                                              >  SRR4302535.2837404/1‑94 (MQ=60)
                                                 CACATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAGCCTGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGCGTACAGCACT                                             <  SRR4302535.2355713/94‑1 (MQ=60)
                                                 CACATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAGCCTGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGCGTACAGCACT                                             >  SRR4302535.3784504/1‑94 (MQ=60)
                                                  ACATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAGCCTGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGCGTACAGCACTT                                            >  SRR4302535.3780129/1‑94 (MQ=60)
                                                      AGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAGCCTGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGCGTACAGCACTTTATC                                        <  SRR4302535.4312521/94‑1 (MQ=60)
                                                      AGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAGCCTGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGCGTACAGCACTTTATC                                        >  SRR4302535.1474511/1‑94 (MQ=60)
                                                      AGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAGCCTGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGCGTACAGCACTTTATC                                        <  SRR4302535.826851/94‑1 (MQ=60)
                                                       GCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAGCCTGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGCGTACAGCACTTTATCG                                       <  SRR4302535.2507163/94‑1 (MQ=60)
                                                       GCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAGCCTGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGCGTACAGCACTTTATCG                                       <  SRR4302535.2287875/94‑1 (MQ=60)
                                                       GCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAGCCTGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGCGTACAGCACTTTATCG                                       <  SRR4302535.1701643/94‑1 (MQ=60)
                                                           TCGCCGTGTTTTTGCATCAATGCGCGTGCACAGCCTGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGCGTACAGCACTTTATCGCCCT                                   <  SRR4302535.2962128/94‑1 (MQ=60)
                                                            CGCCGTGTTTTTGCATCAATGCGCGGGCACAGCCTGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGCGTACAGCACTTTATCGCCCTT                                  <  SRR4302535.4009074/94‑1 (MQ=60)
                                                              CCGTGTTTTTGCATCAATGCGCGGGCACAGCCTGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGCGTACAGCACTTTATCGCCCTTCA                                <  SRR4302535.1955134/94‑1 (MQ=60)
                                                               CGTGTTTTTGCATCAATGCGCGGGCACAGCCTGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGCGTACAGCACTTTATCGCCCTTCAC                               >  SRR4302535.1627666/1‑94 (MQ=60)
                                                               CGTGTTTTTGCATCAATGCGCGGGCACAGCCTGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGCGTACAGCACTTTATCGCCCTTCAC                               >  SRR4302535.1490847/1‑94 (MQ=60)
                                                                GTGTTTTTGCATCAATGCGCGGGCACAGCCTGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGCGTACAGCACTTTATCGCCCTTCACT                              <  SRR4302535.903890/94‑1 (MQ=60)
                                                                GTGTTTTTGCATCAATGCGCGGGCACAGCCTGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGCGTACAGCACTTTATCGCCCTTCACT                              <  SRR4302535.2684054/94‑1 (MQ=60)
                                                                GTGTTTTTGCATCAATGCGCGGGCACAGCCTGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGCGTACAGCACTTTATCGCCCTTCACT                              <  SRR4302535.2687381/94‑1 (MQ=60)
                                                                GTGTTTTTGCATCAATGCGCGGGCACAGCCTGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGCGTACAGCACTTTATCGCCCTTCACT                              >  SRR4302535.3089968/1‑94 (MQ=60)
                                                                GTGTTTTTGCATCAATGCGCGGGCACAGCCTGGGTTGGTTTCGAGGTGCAGAATACGCGAAGCATGGGCGTACAGCACTTTATCGCCCTTCACT                              >  SRR4302535.3089969/1‑94 (MQ=60)
                                                                   TTTTTGCATCAATGCGCGGGCACAGCCTGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGCGTACAGCACTTTATCGCCCTTCACTTTC                           <  SRR4302535.419235/94‑1 (MQ=60)
                                                                    TTTTGCATCAATGCGCGGGCACAGCCTGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGCGTACAGCACTTTATCGCCCTTCACTTTCT                          <  SRR4302535.4141813/94‑1 (MQ=60)
                                                                      TTGCATCAATGCGCGGGCACAGCCTGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGCGTACAGCACTTTATCGCCCTTCACTTTCTCG                        <  SRR4302535.3274484/94‑1 (MQ=60)
                                                                       TGCATCAATGCGCGGGCACAGCCTGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGCGTACAGCACTTTATCGCCCTTCACTTTCTCGA                       <  SRR4302535.1619833/94‑1 (MQ=60)
                                                                        GCATCAATGCGCGGGCACAGCCTGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGCGTACAGCACTTTATCGCCCTTCACTTTCTCGAA                      <  SRR4302535.2197404/94‑1 (MQ=60)
                                                                         CATCAATGCGCGGGCACAGCCTGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGCGTACAGCACTTTATCGCCCTTCACTTTCTCGAAA                     >  SRR4302535.1878436/1‑94 (MQ=60)
                                                                          ATCAATGCGCGGGCACAGCCTGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGCGTACAGCACTTTATCGCCCTTCACTTTCTCGAAAG                    <  SRR4302535.2991534/94‑1 (MQ=60)
                                                                          ATCAATGCGCGGGCACAGCCTGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGCGTACAGCACTTTATCGCCCTTCACTTTCTCGAAAG                    <  SRR4302535.4362592/94‑1 (MQ=60)
                                                                          ATCAATGCGCGGGCACAGCCTGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGCGTACAGCACTTTATCGCCCTTCACTTTCTCGAAAG                    <  SRR4302535.187003/94‑1 (MQ=60)
                                                                          ATCAATGCGCGGGCACAGCCTGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGCGTACAGCACTTTATCGCCCTTCACTTTCTCGAAAG                    <  SRR4302535.485429/94‑1 (MQ=60)
                                                                          ATCAATGCGCGGGCACAGCCTGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGCGTACAGCACTTTATCGCCCTTCACTTTCTCGAAAG                    <  SRR4302535.3219555/94‑1 (MQ=60)
                                                                            CAATGCGCGGGCACAGCCTGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGCGTACAGCACTTTATCGCCCTTCACTTTCTCGAAAGAA                  >  SRR4302535.3667969/1‑94 (MQ=60)
                                                                            CAATGCGCGGGCACAGCCTGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGCGTACAGCACTTTATCGCCCTTCACTTTCTCGAAAGAA                  >  SRR4302535.1827600/1‑94 (MQ=60)
                                                                             AATGCGCGGGCACAGCCTGGGTTTGTTTCGTGGTGCAGAATACGCGAAGCATGGGCGTACAGCACTTTATCGCCCTTCACTTTCTCGAAAGAAG                 <  SRR4302535.4412232/94‑1 (MQ=60)
                                                                             AATGCGCGGGCACAGCCTGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGCGTACAGCACTTTATCGCCCTTCACTTTCTCGAAAGAAG                 <  SRR4302535.3229879/94‑1 (MQ=60)
                                                                               TGCGCGGGCACAGCCTGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGCGTACAGCACTTTATCGCCCTTCACTTTCTCGAAAGAAGGC               <  SRR4302535.1155556/94‑1 (MQ=60)
                                                                               TGCGCGGGCACAGCCTGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGCGTACAGCACTTTATCGCCCTTCACTTTCTCGAAAGAAGGC               <  SRR4302535.3289693/94‑1 (MQ=60)
                                                                               TGCGCGGGCACAGCCTGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGCGTACAGCACTTTATCGCCCTTCACTTTCTCGAAAGAAGGC               <  SRR4302535.498163/94‑1 (MQ=60)
                                                                                GCGCGGGCACAGCCTGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGCGTACAGCACTTTATCGCCCTTCACTTTCTCGAAAGAAGGCA              <  SRR4302535.4672079/94‑1 (MQ=60)
                                                                                GCGCGGGCACAGCCTGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGCGTACAGCACTTTATCGCCCTTCACTTTCTCGAAAGAAGGCA              <  SRR4302535.808544/94‑1 (MQ=60)
                                                                                 GGCGGGCACAGCCTGGGTTGATTTCGGGGTGCATAATACGCGAAGCATGGGCGTACAGCACTTTATCGCCCTTCACTTTCTCGAAAGAAGGCAG             <  SRR4302535.1665619/94‑1 (MQ=60)
                                                                                 CGCGGGCACAGCCTGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGCGTACAGCACTTTATCGCCCTTCACTTTCTCGAAAGAAGGCAG             <  SRR4302535.1150690/94‑1 (MQ=60)
                                                                                     GGCACAGCCTGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGCGTACAGCACTTTATCGCCCTTCACTTTCTCGAAAGAAGGCAGCAAC         <  SRR4302535.4349806/94‑1 (MQ=60)
                                                                                     GGCACAGCCTGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGCGTACAGCACTTTATCGCCCTTCACTTTCTCGAAAGAAGGCAGCAAC         >  SRR4302535.2566109/1‑94 (MQ=60)
                                                                                       CACAGCCTGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGCGTACAGCACTTTATCGCCCTTCACTTTCTCGAGAGAAGGCAGCAACAC       <  SRR4302535.1769386/94‑1 (MQ=60)
                                                                                       CACAGCCTGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGCGTACAGCACTTTATCGCCCTTCACTTTCTCGAAAGAAGGCAGCAACAC       >  SRR4302535.3409134/1‑94 (MQ=60)
                                                                                        ACAGCCTGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGCGTACAGCACTTTATCGTCCTTCACTTTCTCGAAAGAAGGCAGCAACACG      >  SRR4302535.4710222/1‑94 (MQ=60)
                                                                                         CAGCCTGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGCGTACAGCACTTTATCGCCCTTCACTTTCTCGAAAGAAGGCAGCAACACGT     <  SRR4302535.4527416/94‑1 (MQ=60)
                                                                                          agcCTGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGCGTACAGCACTTTATCGCCCTTCACTTTCTCGAAAGAAGGCAGCAACACGTA    >  SRR4302535.2628741/4‑94 (MQ=60)
                                                                                          agcCTGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGCGTACAGCACTTTATCGCCCTTCACTTTCTCGAAAGAAGGCAGCAACACGTA    >  SRR4302535.3934659/4‑94 (MQ=60)
                                                                                           gcCTGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGCGTACAGCACTTTATCGCCCTTCACTTTCTCGAAAGAAGGCAGCAACACGTAG   <  SRR4302535.281402/92‑1 (MQ=60)
                                                                                           C‑CTGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGCGTACAGCACTTTATCGCCCTTCACTTTCTCGAAAGAAGGCAGCAACACGTAGG  >  SRR4302535.3378888/1‑94 (MQ=60)
                                                                                           C‑CTGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGCGTACAGCACTTTATCGCCCTTCACTTTCTCGAAAGAAGGCAGCAACACGTAGG  <  SRR4302535.488877/94‑1 (MQ=60)
                                                                                           C‑CTGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGCGTACAGCACTTTATCGCCCTTCACTTTCTCGAAAGAAGGCAGCAACACGTAGG  <  SRR4302535.1164396/94‑1 (MQ=60)
                                                                                             |                                                                                            
CGCTGTCCATCTCTTCGGTAGAAAGCGGAATAGCAGGCGGGTTGATCCACACATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAG‑CTGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGCGTACAGCACTTTATCGCCCTTCACTTTCTCGAAAGAAGGCAGCAACACGTAGG  >  NC_000913/3158110‑3158294

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑