Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 1696 279.3 15874187 97.8% 15524954 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,158,202 +C coding (961/975 nt) ygiRQ ← Fe‑S oxidoreductase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,158,2011.C99.4% 993.5 / ‑2.0 321coding (962/975 nt)ygiRQFe‑S oxidoreductase
Reads supporting (aligned to +/- strand):  ref base . (0/2);  new base C (147/171);  total (148/173)
Fisher's exact test for biased strand distribution p-value = 5.02e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01

Only 100 of 361 total aligned reads displayed.

ACGCTGTCCATCTCTTCGGTAGAAAGCGGAATAGCAGGCGGGTTGATCCACACATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAG‑CTGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGCGTACAGCACTTTATCGCCCTTCACTTTCTCGAAAGAAGGCAGCAACA  >  NC_000913/3158109‑3158288
                                                                                             |                                                                                       
 cGCTGTCCATCTCTTCGGTAGAAAGCGGAATAGCAGGCGGGTTGATCCACACATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAG‑c                                                                                        <  2:2237537/93‑1 (MQ=255)
 cGCTGTCCATCTCTTCGGTAGAAAGCGGAATAGCAGGCGGGTTGATCCACACATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAG‑c                                                                                        >  2:731960/1‑93 (MQ=255)
  gCTGTCCATCTCTTCGGTAGAAAGCGGAATAGCAGGCGGGTTGATCCACACATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAG‑cc                                                                                       <  2:171511/93‑2 (MQ=255)
   cTGTCCATCTCTTCGGTAGAAAGCGGAATAGCAGGCGGGTTGATCCACACATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAG‑cct                                                                                      <  2:1427307/93‑3 (MQ=255)
   cTGTCCATCTCTTCGGTAGAAAGCGGAATAGCAGGCGGGTTGATCCACACATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAG‑cct                                                                                      <  1:6749035/93‑3 (MQ=255)
        cATCTCTTCGGTAGAAAGCGGAATAGCAGGCGGGTTGATCCACACATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAGCCTGggtt                                                                                  <  1:2592335/93‑1 (MQ=255)
         aTCTCTTCGGTAGAAAGCGGAATAGCAGGCGGGTTGATCCACACATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAGCCTGggttg                                                                                 >  1:7657762/1‑93 (MQ=255)
          tctcTTCGGTAGAAAGCGGAATAGCAGGCGGGTTGATCCACACATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAGACTGggttgg                                                                                >  2:391922/1‑93 (MQ=255)
          tctcTTCGGTAGAAAGCGGAATACCAGGCGGGTTGATCCACACATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAGCCTGggttgg                                                                                <  2:4310262/93‑1 (MQ=255)
           ctctTCGGTAGAAAGCGGAATAGCAGGCGGGTTGATCCACACATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAGCCTGggttggt                                                                               <  2:5430567/93‑1 (MQ=255)
            tctTCGGTAGAAAGCGGAATAGCAGGCGGGTTGATCCACACATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAGCCTGggttggtt                                                                              >  1:3431326/1‑93 (MQ=255)
              ttCGGTAGAAAGCGGAATAGCAGGCGGGTTGATCCACACATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAGCCTGGGTTGGTTTc                                                                            >  2:875740/1‑93 (MQ=255)
              ttCGGTAGAAAGCGAAATAGCAGGCGGGTTGATCCACACATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAGCCTGGGTTGGTTTc                                                                            <  2:4560101/93‑1 (MQ=255)
               tCGGTAGAAAGCGGAATAGCAGGCGGGTTGATCCACACATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAGCCTGGGTTGGTTTCg                                                                           <  2:3485857/93‑1 (MQ=255)
                cGGTAGAAAGCGGAATAGCAGGCGGGTTGATCCACACATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAGCCTGGGTTGGTTTCgt                                                                          <  1:4987825/93‑1 (MQ=255)
                 ggTAGAAAGCGGAATAGCAGGCGGGTTGATCCACACATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAGCCTGGGTTGGTTTCgtg                                                                         >  1:2009983/1‑93 (MQ=255)
                 ggTAGAAAGCGGAATAGCAGGCGGGTTGATCCACACATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAGCCTGGGTTGGTTTCgtg                                                                         >  2:2130637/1‑93 (MQ=255)
                  gTAGAAAGCGGAATAGCAGGCGGGTTGATCCACACATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAGCCTGGGTTGGTTTCgtgg                                                                        >  2:3716603/1‑93 (MQ=255)
                  gTAGAAAGCGGAATAGCAGGCGGGTTGATCCACACATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAGCCTGGGTTGGTTTCgtgg                                                                        <  1:7891541/93‑1 (MQ=255)
                   tAGAAAGCGGAATAGCAGGCGGGTTGATCCACACATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAGCCTGGGTTGGTTTCgtggt                                                                       >  2:3354940/1‑93 (MQ=255)
                     gAAAGCGGAATAGCAGGCGGGTTGATCCACACATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAGCCTGGGTTGGTTTCGTGGTGc                                                                     <  1:6271595/93‑1 (MQ=255)
                     gAAAGCGGAATAGCAGGCGGGTTGATCCACACATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAGCCTGGGTTGGTTTCGTGGTGc                                                                     <  1:4323307/93‑1 (MQ=255)
                     gAAAGCGGAATAGCAGGCGGGTTGATCCACACATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAGCCTGGGTTGGTTTCGTGGTGc                                                                     >  2:6071146/1‑93 (MQ=255)
                        aGCGGAATAGCAGGCGGGTTGATCCACACATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAGCCTGGGTTGGTTTCGTGGTGCAGa                                                                  >  1:1917419/1‑93 (MQ=255)
                         gCGGAATAGCAGGCGGGTTGATCCACACATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAGCCTGGGTTGGTTTCGTGGTGCAGaa                                                                 <  1:3713672/93‑1 (MQ=255)
                         gCGGAATAGCAGGCGGGTTGATCCACACATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAGCCTGGGTTGGTTTCGTGGTGCAGaa                                                                 <  2:6711797/93‑1 (MQ=255)
                           ggAATAGCAGGCGGGTTGATCCACACATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAGCCTGGGTTGGTTTCGTGGTGCAGAATa                                                               <  1:3115462/93‑1 (MQ=255)
                           ggAATAGCAGGCGGGTTGATCCACACATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAGCCTGGGTTGGTTTCGTGGTGCAGAATa                                                               <  2:2884469/93‑1 (MQ=255)
                           ggAATAGCAGGCGGGTTGATCCACACATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAGCCTGGGTTGGTTTCGTGGTGCAGAATa                                                               >  1:658694/1‑93 (MQ=255)
                           ggAATAGCAGGCGGGTTGATCCACACATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAGCCTGGGTTGGTTTCGTGGTGCAGAATa                                                               >  2:3834659/1‑93 (MQ=255)
                           ggAATAGCAGGCGGGTTGATCCACACATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAGCCTGGGTTGGTTTCGTGGTGCAGAATa                                                               >  2:7151891/1‑93 (MQ=255)
                            gAATAGCAGGCGGGTTGATCCACACATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAGCCTGGGTTGGTTTCGTGGTGCAGAATAc                                                              >  1:3610538/1‑93 (MQ=255)
                            gAATAGCAGGCGGGTTGATCCACACATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAGCCTGGGTTGGTTTCGTGGTGCAGAATAc                                                              <  2:2019459/93‑1 (MQ=255)
                               tAGCAGGCGGGTTGATCCACACATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAGCCTGGGTTGGTTTCGTGGTGCAGAATAcgcg                                                           >  1:1040011/1‑93 (MQ=255)
                               tAGCAGGCGGGTTGATCCACACATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAGCCTGGGTTGGTTTCGTGGTGCAGAATAcgcg                                                           >  2:3282038/1‑93 (MQ=255)
                               tAGCAGGCGGGTTGATCCACACATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAGCCTGGGTTGGTTTCGTGGTGCAGAATAcgcg                                                           <  1:2225404/93‑1 (MQ=255)
                               tAGCAGGCGGGTTGATCCACACATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAGCCTGGGTTGGTTTCGTGGTGCAGAATAcgcg                                                           >  1:502347/1‑93 (MQ=255)
                                 gCAGGCGGGTTGATCCACACATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAGCCTGGGTTGGTTTCGTGGTGCAGAATACGCGaa                                                         <  1:7792755/93‑1 (MQ=255)
                                   aGGCGGGTTGATCCACACATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAGCCTGGGTTGGTTTCGTGGTGCAGAATACGCGAAGc                                                       <  2:7423164/93‑1 (MQ=255)
                                   aGGCGGGTTGATCCACACATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAGCCTGGGTTGGTTTCGTGGTGCAGAATACGCGAAGc                                                       >  1:7729518/1‑93 (MQ=255)
                                   aGGCGGGTTGATCCACAAATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAGCCTGGGTTGGTTTCGTGGTGCAGAATACGCGAAGc                                                       <  1:1284281/93‑1 (MQ=255)
                                    ggCGGGTTGATCCACACATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAGCCTGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCa                                                      <  1:6180693/93‑1 (MQ=255)
                                    ggCGGGTTGATCCACACATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAGCCTGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCa                                                      <  1:2364616/93‑1 (MQ=255)
                                    ggCGGGTTGATCCACACATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAGCCTGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCa                                                      <  2:5819744/93‑1 (MQ=255)
                                    ggCGGGGTGATCCACACATAACGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAGCCTGGGATGGTTTCGTGGGGCGGAATACGCGAAGCa                                                      >  2:5584793/1‑93 (MQ=255)
                                     gCGGGTTGATCTACACATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAGCCTGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCAt                                                     <  2:7016439/93‑1 (MQ=255)
                                     gCGGGTTGATCCACACATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAGCCTGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCAt                                                     <  1:1514906/93‑1 (MQ=255)
                                     gCGGGTTGATCCACACATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAGCCTGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCAt                                                     >  2:3000206/1‑93 (MQ=255)
                                     gCGGGTTGATCCACACATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAGCCTGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCAt                                                     <  1:5372788/93‑1 (MQ=255)
                                     gCGGGTTGATCCACACATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAGCCTGGGTTGGTTTCGTGGTGCAGAATACACGAAGCAt                                                     <  2:7582577/93‑1 (MQ=255)
                                      cGGGTTGATCCACACATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAGCCTGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATg                                                    >  2:5929874/1‑93 (MQ=255)
                                       gggTTGATCCACACATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAGCCTGGGTTGGTTTCGTGGGGCAGAATACGCGCAGCATgg                                                   >  1:5563615/1‑93 (MQ=255)
                                        ggTTGATCCACACATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAGCCTGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATggg                                                  >  2:101625/1‑93 (MQ=255)
                                        ggTTGATCCACACATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAGCCTGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATggg                                                  >  2:3634823/1‑93 (MQ=255)
                                        ggTTGATCCACACATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAGCCTGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATggg                                                  <  1:2794660/93‑1 (MQ=255)
                                          ttGATCCACACATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAGCCTGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGCg                                                <  1:4106055/93‑1 (MQ=255)
                                          ttGATCCACACATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAGCCTGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGCg                                                >  2:6919768/1‑93 (MQ=255)
                                          ttGATCCACACATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAGCCTGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGCg                                                >  1:2895114/1‑93 (MQ=255)
                                             aTCCACACATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAGCCTGGGTTGGTTTCGTGGTGCAGAATACGGGAAGCAGGGGCGTAc                                             >  1:2683085/1‑93 (MQ=255)
                                             aTCCACACATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAGCCTGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGCGTAc                                             >  2:1256797/1‑93 (MQ=255)
                                             aTCCACACATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAGCCTGGGTTGGTTTCGTGGTGCAGAATACGCGAAGAATGGGCGTaa                                             >  1:5800972/1‑92 (MQ=255)
                                              tCCACACATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAGCCTGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGCGTACa                                            <  1:7238687/93‑1 (MQ=255)
                                              tCCACACATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAGCCTGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGCGTACa                                            >  1:6515428/1‑93 (MQ=255)
                                               ccACACATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAGCCTGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGCGTACAg                                           <  1:7341472/93‑1 (MQ=255)
                                               ccACACATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAGCCTGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGCGTACAg                                           >  1:6007075/1‑93 (MQ=255)
                                               ccACACATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAGCCTGGGTTGGTTTCGTGGGGCAGAATACGCGAAGCATGGCCGtcaag                                           >  1:2539337/1‑89 (MQ=255)
                                                cacacaTAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAGCCTGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGCGTACAGc                                          >  2:773224/1‑93 (MQ=255)
                                                  cacaTAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAGCCTGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGCGTACAGCAc                                        <  1:4647306/93‑1 (MQ=255)
                                                  cacaTAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAGCCTGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGCGTACAGCAc                                        <  2:4152959/93‑1 (MQ=255)
                                                     aTAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAGCCTGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGCGTACAGCACttt                                     <  1:4558751/93‑1 (MQ=255)
                                                     aTAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAGCCTGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGAGTACAGCACttt                                     >  2:4065157/1‑93 (MQ=255)
                                                      tAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAGCCTGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGCGTACAGCACTTTa                                    <  1:6872741/93‑1 (MQ=255)
                                                      tAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAGCCTGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGCGTACAGCACTTTa                                    <  1:6974046/93‑1 (MQ=255)
                                                       aGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAGCCTGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGCGTACAGCACTTTAt                                   <  1:1717203/93‑1 (MQ=255)
                                                        gCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAGCCTGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGCGTACAGCACTTTATc                                  >  2:2544571/1‑93 (MQ=255)
                                                        gCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAGCCTGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGCGTACAGCACTTTATc                                  <  2:466735/93‑1 (MQ=255)
                                                          ggTCGCCGTGTTTTTGCATCAATGCGCGGGCACAGCCTGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGCGTACAGCACTTTATCGc                                >  1:3460686/1‑93 (MQ=255)
                                                           gTCGCCGTGTTTTTGCATCAATGCGCGGGCACAGCCTGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGCGTACAGCACTTTATCGcc                               >  2:5049825/1‑93 (MQ=255)
                                                             cGCCGTGTTTTTGCATCAATGCGCGGGCACAGCCTGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGCGTACAGCACTTTATCGCCCt                             <  2:2469005/93‑1 (MQ=255)
                                                              gCCGTGTTTTTGCATCAATGCGCGGGCACAGCCTGGGTTGGGTTCGTGGTGCAGAATACGCGAAGCATGGTCGTACAGCACTTTATCGCCCtt                            >  2:849572/1‑93 (MQ=255)
                                                               ccGTGTTTTTGCATCAATGCGCGGGCACAGCCTGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGCGTACAGCACTTTATCGCCCTTc                           >  1:1774477/1‑93 (MQ=255)
                                                               ccGTGTTTTTGCATCAATGCGCGGGCACAGCCTGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGCGTACAGCACTTTATCGCCCTTc                           >  2:1492459/1‑93 (MQ=255)
                                                                cGTGTTTTTGCATCAATGCGCGGGCACAGCCTGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGCGTACAGCACTTTATCGCCCTTCa                          <  2:2740106/93‑1 (MQ=255)
                                                                 gtgtTTTTGCATCAATGCGCGGGCACAGCCTGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGCGTACAGCACTTTATCGCCCTTCAc                         >  1:6426044/1‑93 (MQ=255)
                                                                   gtTTTTGCATCAATGCGCGGGCACAGCCTGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGCGTACAGCACTTTATCGCCCTTCACtt                       <  1:6361520/93‑1 (MQ=255)
                                                                    tttttGCATCAATGCGCGGGCACAGCCTGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGCGTGCAGCACTTTATCGCCCTTCACttt                      >  2:3207395/1‑93 (MQ=255)
                                                                    tttttGCATCAATGCGCGGGCACAGCCTGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGCGTACAGCACTTTATCGCCCTTCACttt                      >  2:5268117/1‑93 (MQ=255)
                                                                          cATCAATGCGCGGGCACAGCCTGGTTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGCGTACAGCACTTTATCGCCCTTCACTTTCTCGaa                <  2:5612484/93‑1 (MQ=255)
                                                                           aTCAATGCGCGGGCACAGCCTGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGCGTACAGCACTTTATCGCCCTTCACTTTCTCGaaa               <  1:382012/93‑1 (MQ=255)
                                                                           aTCAATGCGCGGGCACAGCCTGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGCGTACAGCACTTTATCGCCCTTCACTTTCTCGaaa               <  1:7509789/93‑1 (MQ=255)
                                                                            tCAATGCGCGGGCACAGCCTGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGCGTACAGCACTTTATCGCCCTTCACTTTCTCGAaag              >  2:2373789/1‑93 (MQ=255)
                                                                             cAATGCGCGGGCACAGCCTGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGCGTACAGCACTTTATCGCCCTTCACTTTCTCGAaaga             <  1:1606902/93‑1 (MQ=255)
                                                                             cAATGCGCGGGCACAGCCTGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGCGTACAGCACTTTATCGCCCTTCACTTTCTCGAaaga             <  2:644406/93‑1 (MQ=255)
                                                                              aaTGCGCGGGCACAGCCTGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGCGTACAGCACTTTATCGCCCTTCACTTTCTCGAaagaa            <  2:135698/93‑1 (MQ=255)
                                                                                 gcgcGGGCACAGCCTGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGCGTACAGCACTTTATCGCCCTTCACTTTCTCGAAAGAAGgc         <  2:4808145/93‑1 (MQ=255)
                                                                                  cgcgGGCACAGCCTGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGCGTACAGCACTTTATCGCCCTTCACTTTCTCGAAAGAAGgca        >  2:5704920/1‑93 (MQ=255)
                                                                                   gcgGGCACAGCCTGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGCGTACAGCACTTTATCGCCCTTCACTTTCTCGAAAGAAGgcag       >  2:6248984/1‑93 (MQ=255)
                                                                                      ggCACAGCCTGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGCGTACAGCACTTTATCGCCCTTCACTTTCTCGAAAGAAGGCAGCaa    >  1:2301149/1‑93 (MQ=255)
                                                                                      ggCACAGCCTGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGCGTACAGCACTTTATCGCCCTTCACTTTCTCGAAAGAAGGCAGCaa    <  1:3626673/93‑1 (MQ=255)
                                                                                      ggCACAGCCTGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGCGTACAGCACTTTATCGCCCTTCACTTTCTCGAAAGAAGGCAGCaa    <  1:1143085/93‑1 (MQ=255)
                                                                                             |                                                                                       
ACGCTGTCCATCTCTTCGGTAGAAAGCGGAATAGCAGGCGGGTTGATCCACACATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAG‑CTGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGCGTACAGCACTTTATCGCCCTTCACTTTCTCGAAAGAAGGCAGCAACA  >  NC_000913/3158109‑3158288

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
ACGCTGTCCATCTCTTCGGTAGAAAGCGGAATAGCAGGCGGGTTGATCCACACATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAG‑C‑TGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGCGTACAGCACTTTATCGCCCTTCACTTTCTCGAAAGAAGGCAGCAACACGTAGGT  >  NC_000913/3158109‑3158295
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ACGCTGTCCATCTCTTCGGTAGAAAGCGGAATAGCAGGCGGGTTGATCCACACATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAG‑C                                                                                                <  SRR4302543.2110282/94‑1 (MQ=60)
ACGCTGTCCATCTCTTCGGTAGAAAGCGGAATAGCAGGCGGGTTGATCCACACATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAG‑C                                                                                                <  SRR4302543.759766/94‑1 (MQ=60)
  GCTGTCCATCTCTTCGGTAGAAAGCGGAATAGCAGGCGGGTTGATCCACACATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAG‑Cct                                                                                              <  SRR4302543.7946950/94‑3 (MQ=60)
   CTGTCCATCTCTTCGGTAGAAAGCGGAATAGCAGGCGGGTTGATCCACACATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAG‑Cctg                                                                                             <  SRR4302543.3819069/94‑4 (MQ=60)
   CTGTCCATCTCTTCGGTAGAAAGCGGAATAGCAGGCGGGTTGATCCACACATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAG‑Cctg                                                                                             <  SRR4302543.5429767/94‑4 (MQ=60)
   CTGTCCATCTCTTCGGTAGAAAGCGGAATAGCAGGCGGGTTGATCCACACATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAG‑Cctg                                                                                             <  SRR4302543.6812769/94‑4 (MQ=60)
    TGTCCATCTCTTCGGTAGAAAGCGGAATAGCAGGCGGGTTGATCCACACATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAGCC‑TGG                                                                                            >  SRR4302543.4590155/1‑94 (MQ=60)
     GTCCATCTCTTCGGTAGAAAGCGGAATAGCAGGCGGGTTGATCCACACATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAGCC‑TGGG                                                                                           >  SRR4302543.6154192/1‑94 (MQ=60)
        CATCTCTTCGGTAGAAAGCGGAATAGCAGGCGGGTTGATCCACACATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAGCC‑TGGGTTG                                                                                        <  SRR4302543.2615891/94‑1 (MQ=60)
        CATCTCTTCGGTAGAAAGCGGAATAGCAGGCGGGTTGATCCACACATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAGCC‑TGGGTTG                                                                                        >  SRR4302543.6937854/1‑94 (MQ=60)
        CATCTCTTCGGTAGAAAGCGGAATAGCAGGCGGGTTGATCCACACATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAGCC‑TGGGTTG                                                                                        >  SRR4302543.7730355/1‑94 (MQ=60)
        CATCTCTTCGGTAGAAAGCGGAATAGCAGGCGGGTTGATCCACACATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAGCC‑TGGGTTG                                                                                        >  SRR4302543.858194/1‑94 (MQ=60)
         ATCTCTTCGGTAGAAAGCGGAATAGCAGGCGGGTTGATCCACACATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAGCC‑TGGGTTGG                                                                                       <  SRR4302543.139523/94‑1 (MQ=60)
         ATCTCTTCGGTAGAAAGCGGAATAGCAGGCGGGTTGATCCACACATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAGCC‑TGGGTTGG                                                                                       >  SRR4302543.6844128/1‑94 (MQ=60)
           CTCTTCGGTAGAAAGCGGAATAGCAGGCGGGTTGATCCACACATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAGCC‑TGGGTTGGTT                                                                                     <  SRR4302543.6746999/94‑1 (MQ=60)
           CTCTTCGGTAGAAAGCGGAATAGCAGGCGGGTTGATCCACACATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAGCC‑TGGGTTGGTT                                                                                     >  SRR4302543.3462029/1‑94 (MQ=60)
           CTCTTCGGTAGAAAGCGGAATAGCAGGCGGGTTGATCCACACATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAGCC‑TGGGTTGGTT                                                                                     >  SRR4302543.1958508/1‑94 (MQ=60)
           CTCTTCGGTAGAAAGCGGAATAGCAGGCGGGTTGATCCACACATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAGCC‑TGGGTTGGTT                                                                                     >  SRR4302543.5047470/1‑94 (MQ=60)
           CTCTTCGGTAGAAAGCGGAATAGCAGGCGGGTTGATCCACACATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAGCC‑TGGGTTGGTT                                                                                     >  SRR4302543.691707/1‑94 (MQ=60)
           CTCTTCGGTAGAAAGCGGAATAGCAGGCGGGTTGATCCACACATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAGCC‑TGGGTTGGTT                                                                                     >  SRR4302543.1808902/1‑94 (MQ=60)
           CTCTTCGGTAGAAAGCGGAATAGCAGGCGGGTCGATCCACACATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAGCC‑TGGGTTGGTT                                                                                     >  SRR4302543.3462030/1‑94 (MQ=60)
            TCTTCGGTAGAAAGCGGAATAGCAGGCGGGTTGATCCACACATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAGCC‑TGGGTTGGTTT                                                                                    <  SRR4302543.2192976/94‑1 (MQ=60)
            TCTTCGGTAGAAAGCGGAATAGCAGGCGGGTTGATCCACACATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAGCC‑TGGGTTGGTTT                                                                                    >  SRR4302543.4207624/1‑94 (MQ=60)
             CTTCGGTAGAAAGCGGAATAGCAGGCGGGTTGATCCACACATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAGCC‑TGGGTTGGTTTC                                                                                   >  SRR4302543.5020873/1‑94 (MQ=60)
             CTTCGGTAGAAAGCGGAATAGCAGGCGGGTTGATCCACACATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAGCC‑TGGGTTGGTTTC                                                                                   >  SRR4302543.6250437/1‑94 (MQ=60)
              TTCGGTAGAAAGCGGAATAGCAGGCGGGTTGATCCACACATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAGCC‑TGGGTTGGTTTCG                                                                                  >  SRR4302543.7820570/1‑94 (MQ=60)
               TCGGTAGAAAGCGGAATAGCAGGCGGGTTGATCCACACATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAGCC‑TGGGTTGGTTTCGT                                                                                 <  SRR4302543.3737111/94‑1 (MQ=60)
                CGGTAGAAAGCGGAATAGCAGGCGGGTTGATCCACACATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAGCC‑TGGGTTGGTTTCGTG                                                                                <  SRR4302543.5033232/94‑1 (MQ=60)
                CGGTAGAAAGCGGAATAGCAGGCGGGTTGATCCACACATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAGCC‑TGGGTTGGTTTCGTG                                                                                >  SRR4302543.2028044/1‑94 (MQ=60)
                 GGTAGAAAGCGGAATAGCAGGCGGGTTGATCCACACATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAGCC‑TGGGTTGGTTTCGTGG                                                                               >  SRR4302543.3645058/1‑94 (MQ=60)
                  GTAGAAAGCGGAATAGCAGGCGGGTTGATCCACACATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAGCC‑TGGGTTGGTTTCGTGGT                                                                              <  SRR4302543.7965753/94‑1 (MQ=60)
                  GTAGAAAGCGGAATAGCAGGCGGGTTGATCCACACATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAGCC‑TGGGTTGGTTTCGTGGT                                                                              <  SRR4302543.1071055/94‑1 (MQ=60)
                   TAGAAAGCGGAATAGCAGGCGGGTTGATCCACACATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAGCC‑TGGGTTGGTTTCGTGGTG                                                                             >  SRR4302543.5181917/1‑94 (MQ=60)
                   TAGAAAGCGGAATAGCAGGCGGGTTGATCCACACATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAGCC‑TGGGTTGGTTTCGTGGTG                                                                             >  SRR4302543.5915582/1‑94 (MQ=60)
                    AGAAAGCGGAATAGCAGGCGGGTTGATCCACACATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAGCC‑TGGGTTGGTTTCGTGGTGC                                                                            >  SRR4302543.6411259/1‑94 (MQ=60)
                    AGAAAGCGGAATAGCAGGCGGGTTGATCCACACATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAGCC‑TGGGTTGGTTTCGTGGTGC                                                                            <  SRR4302543.2697026/94‑1 (MQ=60)
                     GAAAGCGGAATAGCAGGCGGGTTGATCCACACATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAGCC‑TGGGTTGGTTTCGTGGTGCA                                                                           <  SRR4302543.4362558/94‑1 (MQ=60)
                     GAAAGCGGAATAGCAGGCGGGTTGATCCACACATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAGCC‑TGGGTTGGTTTCGTGGTGCA                                                                           <  SRR4302543.6330728/94‑1 (MQ=60)
                     GAAAGCGGAATAGCAGGCGGGTTGATCCACACATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAGCC‑TGGGTTGGTTTCGTGGTGCA                                                                           <  SRR4302543.4362559/94‑1 (MQ=60)
                       AAGCGGAATAGCAGGCGGGTTGATCCACACATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCCCAGCC‑TGGGTTGGTTTCGTGGTGCAGA                                                                         <  SRR4302543.7864419/94‑1 (MQ=60)
                       AAGCGGAATAGCAGGCGGGTTGATCCACACATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAGCC‑TGGGTTGGTTTCGTGGTGCAGA                                                                         >  SRR4302543.1934705/1‑94 (MQ=60)
                        AGCGGAATAGCAGGCGGGTTGATCCACACATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAGCC‑TGGGTTGGTTTCGTGGTGCAGAA                                                                        <  SRR4302543.2611470/94‑1 (MQ=60)
                        AGCGGAATAGCAGGCGGGTTGATCCACACATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAGCC‑TGGGTTGGTTTCGTGGTGCAGAA                                                                        <  SRR4302543.7155439/94‑1 (MQ=60)
                         GCGGAATAGCAGGCGGGTTGATCCACACATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAGCC‑TGGGTTGGTTTCGTGGTGCAGAAT                                                                       <  SRR4302543.3746335/94‑1 (MQ=60)
                         GCGGAATAGCAGGCGGGTTGATCCAAACATAGCGGGCGCCGTGTTTTTGCATCAATGCGCGGGCACAG‑CATGGGTGGGTTTCGTGGTGCAGAAT                                                                       <  SRR4302543.1674301/94‑1 (MQ=60)
                          CGGAATAGCAGGCGGGTTGATCCACACATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAGCC‑TGGGTTGGTTTCGTGGTGCAGAATA                                                                      >  SRR4302543.664745/1‑94 (MQ=60)
                          CGGAATAGCAGGCGGGTTGATCCACACATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAGCC‑TGGGTTGGTTTCGTGGTGCAGAATA                                                                      <  SRR4302543.2824686/94‑1 (MQ=60)
                          CGGAATAGCAGGCGGGTTGATCCACACATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAGCC‑TGGGTTGGTTTCGTGGTGCAGAATA                                                                      >  SRR4302543.7626332/1‑94 (MQ=60)
                          CGGAATAGCAGGCGGGTTGATCCACACATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAGCC‑TGGGGTGGGTTCGTGGTGCAGAATC                                                                      >  SRR4302543.2954661/1‑94 (MQ=60)
                           GGAATAGCAGGCGGGTTGATCCACACATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAGCC‑TGGGTTGGTTTCGTGGTGCAGAATAC                                                                     >  SRR4302543.3642384/1‑94 (MQ=60)
                           GGAATAGCAGGCGGGTTGATCCACACATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAGCC‑TGGGTTGGTTTCGTGGTGCAGAATAC                                                                     >  SRR4302543.3642382/1‑94 (MQ=60)
                           GGAATAGCAGGCGGGTTGATCCACACATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAGCC‑TGGGTTGGTTTCGTGGTGCAGAATAC                                                                     <  SRR4302543.3143484/94‑1 (MQ=60)
                            GAATAGCAGGCGGGTTGATCCACACATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAGCC‑TGGGTTGGTTTCGTGGTGCAGAATACG                                                                    <  SRR4302543.1625829/94‑1 (MQ=60)
                            GAATAGCAGGCGGGTTGATCCACACATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAGCC‑TGGGTTGGTTTCGTGGTGCAGAATACG                                                                    >  SRR4302543.6447807/1‑94 (MQ=60)
                              ATAGCAGGCGGGTTGATCCACACATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAGCC‑TGGGTTGGTTTCGTGGTGCAGAATACGCG                                                                  >  SRR4302543.1049274/1‑94 (MQ=60)
                              ATAGCAGGCGGGTTGATCCACACATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAGCC‑TGGGTTGGTTTCGTGGTGCAGAATACGCG                                                                  >  SRR4302543.507009/1‑94 (MQ=60)
                              ATAGCAGGCGGGTTGATCCACACATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAGCC‑TGGGTTGGTTTCGTGGTGCAGAATACGCG                                                                  <  SRR4302543.6012081/94‑1 (MQ=60)
                              ATAGCAGGCGGGTTGATCCACACATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAGCC‑TGGGTTGGTTTCGTGGTGCAGAATACGCG                                                                  <  SRR4302543.383651/94‑1 (MQ=60)
                              ATAGCAGGCGGGTTGATCCACACATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAGCC‑TGGGTTGGTTTCGTGGTGCAGAATACGCG                                                                  <  SRR4302543.7784909/94‑1 (MQ=60)
                               TAGCAGGCGGGTTGATCCACACATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAGCC‑TGGGTTGGTTTCGTGGTGCAGAATACGCGA                                                                 <  SRR4302543.2245494/94‑1 (MQ=60)
                               TAGCAGGCGGGTTGATCCACACATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAGCC‑TGGGTTGGTTTCGTGGTGCAGAATACGCGA                                                                 >  SRR4302543.6579932/1‑94 (MQ=60)
                                 GCAGGCGGGTTGATCCACACATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAGCC‑TGGGTTGGTTTCGTGGTGCAGAATACGCGAAG                                                               >  SRR4302543.963553/1‑94 (MQ=60)
                                 GCAGGCGGGTTGATCCACACATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAGCC‑TGGGTTGGTTTCGTGGTGCAGAATACGCGAAG                                                               <  SRR4302543.7866198/94‑1 (MQ=60)
                                  CAGGCGGGTTGATCCACACATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAGCC‑TGGGTTGGTTTCGTGGTGCAGAATACGCGAAGC                                                              >  SRR4302543.7802576/1‑94 (MQ=60)
                                  CAGGCGGGTTGATCCACACATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAGCC‑TGGGTTGGTTTCGTGGTGCAGAATACGCGAAGC                                                              >  SRR4302543.3501580/1‑94 (MQ=60)
                                   AGGCGGGTTGATCCACACATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAGCC‑TGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCA                                                             <  SRR4302543.1295729/94‑1 (MQ=60)
                                   AGGCGGGTTGATCCACACATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAGCC‑TGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCA                                                             <  SRR4302543.2732565/94‑1 (MQ=60)
                                   AGGCGGGTTGATCCACAAATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAGCC‑TGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCA                                                             <  SRR4302543.1295728/94‑1 (MQ=60)
                                    GGCGGGTTGATCCACACATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAGCC‑TGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCAT                                                            <  SRR4302543.6239113/94‑1 (MQ=60)
                                    GGCGGGTTGATCCACACATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAGCC‑TGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCAT                                                            <  SRR4302543.2386057/94‑1 (MQ=60)
                                     ttccgatctccactctATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAGCC‑TGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATG                                                           <  SRR4302543.3478404/78‑1 (MQ=60)
                                     GCGGGTTGATCCACACATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAGCC‑TGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATG                                                           <  SRR4302543.5421448/94‑1 (MQ=60)
                                     GCGGGTTGATCCACACATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAGCC‑TGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATG                                                           <  SRR4302543.4224445/94‑1 (MQ=60)
                                     GCGGGTTGATCCACACATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAGCC‑TGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATG                                                           <  SRR4302543.1528511/94‑1 (MQ=60)
                                      CGGGTTGATCCACACATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAGCC‑TGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGG                                                          <  SRR4302543.1865609/94‑1 (MQ=60)
                                      CGGGTTGATCCACACATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAGCC‑TGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGG                                                          >  SRR4302543.7142685/1‑94 (MQ=60)
                                      CGGGTTGATCCACACATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAGCC‑TGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGG                                                          <  SRR4302543.3224957/94‑1 (MQ=60)
                                      CGGGTTGATCCACACATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAGCC‑TGGGTTGGTTTCGTGGGGCAGAATACGCGCAGCATGG                                                          >  SRR4302543.5614217/1‑94 (MQ=60)
                                        GGTTGATCCACACATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAGCC‑TGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGC                                                        <  SRR4302543.2820195/94‑1 (MQ=60)
                                        GGTTGATCCACACATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAGCC‑TGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGC                                                        <  SRR4302543.6559129/94‑1 (MQ=60)
                                         GTTGATCCACACATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAGCC‑TGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGCG                                                       >  SRR4302543.2921438/1‑94 (MQ=60)
                                          TTGATCCACACATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAGCC‑TGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGCGT                                                      >  SRR4302543.4638521/1‑94 (MQ=60)
                                          TTGATCCACACATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAGCC‑TGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGCGT                                                      <  SRR4302543.4142990/94‑1 (MQ=60)
                                           TGATCCACACATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAGCC‑TGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGCGTA                                                     >  SRR4302543.7669113/1‑94 (MQ=60)
                                            GATCCACACATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAGCC‑TGGGTTGGTTTCGTGGTGCAGAATACGGGAAGCAGGGGCGTAC                                                    >  SRR4302543.2707402/1‑94 (MQ=60)
                                            GATCCACACATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAGCC‑TGGGTTGGTTTCGTGGTGCAGAATACGCGAAGAATGGGCGTAA                                                    >  SRR4302543.5853737/1‑94 (MQ=60)
                                             ATCCACACATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAGCC‑TGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGCGTGCA                                                   >  SRR4302543.3578894/1‑94 (MQ=60)
                                             ATCCACACATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAGCC‑TGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGCGTACA                                                   >  SRR4302543.6576793/1‑94 (MQ=60)
                                             ATCCACACATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAGCC‑TGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGCGTACA                                                   >  SRR4302543.6576794/1‑94 (MQ=60)
                                              TCCACACATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAGCC‑TGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGCGTACAG                                                  <  SRR4302543.2805774/94‑1 (MQ=60)
                                              TCCACACATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAGCC‑TGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGCGTACAG                                                  <  SRR4302543.7308762/94‑1 (MQ=60)
                                              TCCACACATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAGCC‑TGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGCGTACAG                                                  >  SRR4302543.6062389/1‑94 (MQ=60)
                                              TCCACACATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAGCC‑TGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGCGTACAG                                                  >  SRR4302543.7558168/1‑94 (MQ=60)
                                              TCCACACATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAGCC‑TGGGTTGGTTTCGTGGGGCAGAATACGCGAAGCATGGccgtcaag                                                  >  SRR4302543.2562484/1‑86 (MQ=60)
                                               CCACACATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAGCC‑TGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGCGTACAGC                                                 <  SRR4302543.3669503/94‑1 (MQ=60)
                                               CCACACATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAGCC‑TGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGCGTACAGC                                                 <  SRR4302543.7412098/94‑1 (MQ=60)
                                                CACACATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAGCC‑TGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGCGTACAGCA                                                >  SRR4302543.6983788/1‑94 (MQ=60)
                                                CACACATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAGCC‑TGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGCGTACAGCA                                                >  SRR4302543.3522834/1‑94 (MQ=60)
                                                  CACATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAGCC‑TGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGCGTACAGCACT                                              <  SRR4302543.6350766/94‑1 (MQ=60)
                                                  CACATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAGCC‑TGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGCGTACAGCACT                                              >  SRR4302543.2257712/1‑94 (MQ=60)
                                                  CACATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAGCC‑TGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGCGTACAGCACT                                              <  SRR4302543.4689591/94‑1 (MQ=60)
                                                  CACATAGCGGTCGCCGTGGTTTTGCATCAATGCGCGGGCACAGCC‑TGGGGTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGCGTACAGCACT                                              >  SRR4302543.7834562/1‑94 (MQ=60)
                                                   ACATATCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAGCC‑TGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGCGTACAGCACTT                                             >  SRR4302543.452549/1‑94 (MQ=60)
                                                   ACATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAGCC‑TGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGCGTGCAGCACTT                                             <  SRR4302543.4049966/94‑1 (MQ=60)
                                                   ACATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAGCC‑TGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGCGTACAGCACTT                                             <  SRR4302543.2296182/94‑1 (MQ=60)
                                                    CATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAGCC‑TGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGCGTACAGCACTTT                                            >  SRR4302543.2297931/1‑94 (MQ=60)
                                                     ATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAGCC‑TGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGCGTACAGCACTTTA                                           <  SRR4302543.4600255/94‑1 (MQ=60)
                                                      TAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAGCC‑TGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGCGTACAGCACTTTAT                                          <  SRR4302543.2491367/94‑1 (MQ=60)
                                                      TAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAGCC‑TGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGCGTACAGCACTTTAT                                          <  SRR4302543.6156120/94‑1 (MQ=60)
                                                      TAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAGCC‑TGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGCGTACAGCACTTTAT                                          <  SRR4302543.6937461/94‑1 (MQ=60)
                                                      TAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAGCC‑TGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGCGTACAGCACTTTAT                                          <  SRR4302543.642697/94‑1 (MQ=60)
                                                      TAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAGCC‑TGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGCGTACAGCACTTTAT                                          >  SRR4302543.1788174/1‑94 (MQ=60)
                                                      TAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAGCC‑TGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGCGTACAGCACTTTAT                                          <  SRR4302543.5852914/94‑1 (MQ=60)
                                                      TAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAGCC‑TGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGCGTACAGCACTTTAT                                          <  SRR4302543.1994135/94‑1 (MQ=60)
                                                      TAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAGCC‑TGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGCGTACAGCACTTTAT                                          <  SRR4302543.7039674/94‑1 (MQ=60)
                                                       AGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAGCC‑TGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGCGTACAGCACTTTATC                                         <  SRR4302543.2677514/94‑1 (MQ=60)
                                                       AGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAGCC‑TGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGCGTACAGCACTTTATC                                         <  SRR4302543.1732543/94‑1 (MQ=60)
                                                       AGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAGCC‑TGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGCGTACAGCACTTTATC                                         <  SRR4302543.3766349/94‑1 (MQ=60)
                                                       AGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAGCC‑TGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGCGTACAGCACTTTATC                                         <  SRR4302543.6809951/94‑1 (MQ=60)
                                                       AGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAGCC‑TGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGCGTACAGCACTTTATC                                         <  SRR4302543.2677515/94‑1 (MQ=60)
                                                       AGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAGCC‑TGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGCGTACAGCACTTTATC                                         <  SRR4302543.1146126/94‑1 (MQ=60)
                                                        GCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAGCC‑TGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGCGTACAGCACTTTATCG                                        <  SRR4302543.6578438/94‑1 (MQ=60)
                                                        GCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAGCC‑TGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGCGTACAGCACTTTATCG                                        <  SRR4302543.4669597/94‑1 (MQ=60)
                                                        GCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAGCC‑TGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGCGTACAGCACTTTATCG                                        <  SRR4302543.7239250/94‑1 (MQ=60)
                                                         CGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAGCC‑TGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGCGTACAGCACTTTATCGC                                       >  SRR4302543.3491686/1‑94 (MQ=60)
                                                          GGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAGCC‑TGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGCGTACAGCACTTTATCGCC                                      >  SRR4302543.6020049/1‑94 (MQ=60)
                                                            TCGCCGTGTTTTTGCATCAATGCGCGGGCACAGCC‑TGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGCGTGCAGCACTTTATCGCCCT                                    <  SRR4302543.4821245/94‑1 (MQ=60)
                                                            TCGCCGTGTTTTTGCATCAATGCGCGGGCACAGCC‑TGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGCGTACAGCACTTTATCGCCCT                                    <  SRR4302543.5678014/94‑1 (MQ=60)
                                                             CGCCGTGTTTTTGCATCAATGCGCGGGCACAGCC‑TGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGCGTACAGCACTTTATCGCCCTT                                   <  SRR4302543.1592382/94‑1 (MQ=60)
                                                              GCCGTGTTTTTGCATCAATGCGCGGGCACAGCC‑TGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGCGTACAGCACTTTATCGCCCTTC                                  >  SRR4302543.7422917/1‑94 (MQ=60)
                                                              GCCGTGTTTTTGCATCAATGCGCGGGCACAGCC‑TGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGCGTACAGCACTTTATCGCCCTTC                                  >  SRR4302543.1790338/1‑94 (MQ=60)
                                                               CCGTGTTTTTGCATCAATGCGCGGGCACAGCC‑TGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGCGTACAGCACTTTATCGCCCTTCA                                 <  SRR4302543.1231972/94‑1 (MQ=60)
                                                               CCGTGTTTTTGCATCAATGCGCGGGCACAGCC‑TGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGCGTACAGCACTTTATCGCCCTTCA                                 <  SRR4302543.2552051/94‑1 (MQ=60)
                                                                CGTGTTTTTGCATCAATGCGCGGGCACAGCC‑TGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGCGTACAGCACTTTATCGCCCTTCAC                                >  SRR4302543.6486597/1‑94 (MQ=60)
                                                                CGTGTTTTTGCATCAATGCGCGGGCACAGCC‑TGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGCGTACAGCACTTTATCGCCCTTCAC                                <  SRR4302543.2974566/94‑1 (MQ=60)
                                                                CGTGTTTTTGCATCAATGCGCGGGCACAGCC‑TGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGCGTACAGCACTTTATCGCCCTTCAC                                >  SRR4302543.5045324/1‑94 (MQ=60)
                                                                CGTGTTTTTGCATCAATGCGCGGGCACAGCC‑TGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGCGTACAGCACTTTATCGCCCTTCAC                                <  SRR4302543.2974384/94‑1 (MQ=60)
                                                                CGTGTTTTTGCATCAATGCGCGGGCACAGCC‑TGGGTTGGTTTCGTGGCGCAGAATACGCGAAGCATGGGCGTACAGCACTTTATCGCCCTTCAC                                >  SRR4302543.6772928/1‑94 (MQ=60)
                                                                 GTGTTTTTGCATCAATGCGCGGGCACAGCC‑TGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGCGTACAGCACTTTATCGCCCTTCACT                               >  SRR4302543.2583624/1‑94 (MQ=60)
                                                                 GTGTTTTTGCATCAATGCGCGGGCACAGCC‑TGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGCGTACAGCACTTTATCGCCCTTCACT                               <  SRR4302543.734785/94‑1 (MQ=60)
                                                                   GTTTTTGCATCAATGCGCGGGCACAGCC‑TGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGCGTACAGCACTTTATCGCCCTTCACTTT                             <  SRR4302543.6421339/94‑1 (MQ=60)
                                                                    TTTTTGCATCAATGCGCGGGCACAGCC‑TGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGCGTACAGCACTTTATCGCCCTTCACTTTC                            <  SRR4302543.5681601/94‑1 (MQ=60)
                                                                      TTTGCATCAATGCGCGGGCACAGCC‑TGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGCGTACAGCACTTTATCGCCCTTCACTTTCTC                          >  SRR4302543.2779578/1‑94 (MQ=60)
                                                                       TTGCATCAATGCGCGGGCACAGCC‑TGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGCGTACAGCACTTTATCGCCCTTCACTTTCTCG                         <  SRR4302543.6923049/94‑1 (MQ=60)
                                                                         GCATCAATGCGCGGGCACAGCC‑TGGTTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGCGTACAGCACTTTATCGCCCTTCACTTTCTCGAA                       <  SRR4302543.1783922/94‑1 (MQ=60)
                                                                         GCATCAATGCGCGGGCACAGCC‑TGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGCGTACAGCACTTTATCGCCCTTCACTTTCTCGAA                       >  SRR4302543.5459138/1‑94 (MQ=60)
                                                                         GCATCAATGCGCGGGCACAGCC‑TGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGCGTACAGCACTTTATCGCCCTTCACTTTCTCGAA                       <  SRR4302543.7568284/94‑1 (MQ=60)
                                                                           ATCAATGCGCGGGCACAGCC‑TGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGCGTACAGCACTTTATCGCGCTTCACTTTCTCGAAAG                     >  SRR4302543.2824604/1‑94 (MQ=60)
                                                                           ATCAATGCGCGGGCACAGCC‑TGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGCGTACAGCACTTTATCGCCCTTCACTTTCTCGAAAG                     >  SRR4302543.2361035/1‑94 (MQ=60)
                                                                           ATCAATGCGCGGGCACAGCC‑TGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGCGTACAGCACTTTATCGCCCTTCACTTTCTCGAAAG                     <  SRR4302543.7757633/94‑1 (MQ=60)
                                                                           ATCAATGCGCGGGCACAGCC‑TGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGCGTACAGCACTTTATCGCCCTTCACTTTCTCGAAAG                     <  SRR4302543.7581543/94‑1 (MQ=60)
                                                                           ATCAATGCGCGGGCACAGCC‑TGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGCGTACAGCACTTTATCGCCCTTCACTTTCTCGAAAG                     >  SRR4302543.1155571/1‑94 (MQ=60)
                                                                           ATCAATGCGCGGGCACAGCC‑TGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGCGTACAGCACTTTATCGCCCTTCACTTTCTCGAAAG                     <  SRR4302543.2241055/94‑1 (MQ=60)
                                                                           ATCAATGCGCGGGCACAGCC‑TGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGCGTACAGCACTTTATCGCCCTTCACTTTCTCGAAAG                     <  SRR4302543.6516538/94‑1 (MQ=60)
                                                                           ATCAATGCGCGGGCACAGCC‑TGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGCGTACAGCACTTTATCGCCCTTCACTTTCTCGAAAG                     >  SRR4302543.4605904/1‑94 (MQ=60)
                                                                           ATCAATGCGCGGGCACAGCC‑TGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGCGTACAGCACTTTATCGCCCTTCACTTTCTCGAAAG                     >  SRR4302543.5280987/1‑94 (MQ=60)
                                                                           ATCAATGCGCGGGCACAGCC‑TGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGCGTACAGCACTTTATCGCCCTTCACTTTCTCGAAAG                     <  SRR4302543.385539/94‑1 (MQ=60)
                                                                             CAATGCGCGGGCACAGCC‑TGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGCGTACAGCACTTTATCGCCCTTCACTTTCTCGAAAGAA                   >  SRR4302543.7327490/1‑94 (MQ=60)
                                                                             CAATGCGCGGGCACAGCC‑TGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGCGTACAGCACTTTATCGCCCTTCACTTTCTCGAAAGAA                   >  SRR4302543.3692977/1‑94 (MQ=60)
                                                                             CAATGCGCGGGCACAGCC‑TGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGCGTACAGCACTTTATCGCCCTTCACTTTCTCGAAAGAA                   <  SRR4302543.630677/94‑1 (MQ=60)
                                                                             CAATGCGCGGGCACAGCC‑TGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGCGTACAGCACTTTATCGCCCTTCACTTTCTCGAAAGAA                   <  SRR4302543.390875/94‑1 (MQ=60)
                                                                             CAATGCGCGGGCACAGCC‑TGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGCGTACAGCACTTTATCGCCCTTCACTTTCTCGAAAGAA                   >  SRR4302543.1570081/1‑94 (MQ=60)
                                                                             CAATGCGCGGGCACAGCC‑TGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGCGTACAGCACTTTATCGCCCTTCACTTTCTCGAAAGAA                   <  SRR4302543.1621232/94‑1 (MQ=60)
                                                                             CAATGCGCGGGCACAGCC‑TGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGCGTACAGCACTTTATCGCCCTTCACTTTCTCGAAAGAA                   <  SRR4302543.1621231/94‑1 (MQ=60)
                                                                              AATGCGCGGGCACAGCC‑TGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGCGTACAGCACTTTATCGCCCTTCACTTTCTCGAAAGAAG                  <  SRR4302543.62230/94‑1 (MQ=60)
                                                                              AATGCGCGGGCACAGCC‑TGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGCGTACAGCACTTTATCGCCCTTCACTTTCTCGAAAGAAG                  >  SRR4302543.2438086/1‑94 (MQ=60)
                                                                              AATGCGCGGGCACAGCC‑TGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGCGTACAGCACTTTATCGCCCTTCACTTTCTCGAAAGAAG                  <  SRR4302543.7929263/94‑1 (MQ=60)
                                                                              AATGCGCGGGCACAGCC‑TGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGCGTACAGCACTTTATCGCCCTTCACTTTCTCGAAAGAAG                  <  SRR4302543.1897012/94‑1 (MQ=60)
                                                                              AATGCGCGGGCACAGCC‑TGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGCGTACAGCACTTTATCGCCCTTCACTTTCTCGAAAGAAG                  >  SRR4302543.5105698/1‑94 (MQ=60)
                                                                              AATGCGCGGGCACAGCC‑TGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGCGTACAGCACTTTATCGCCCTTCACTTTCTCGAAAGAAG                  >  SRR4302543.6449160/1‑94 (MQ=60)
                                                                               ATGCGCGGGCACAGCC‑TGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGCGTACAGCACTTTATCGCCCTTCACTTTCTCGAAAGAAGG                 >  SRR4302543.3314770/1‑94 (MQ=60)
                                                                                 GCGCGGGCACAGCC‑TGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGCGTACAGCACTTTATCGCCCTTCACTTTCTCGAAAGAAGGCA               <  SRR4302543.1644700/94‑1 (MQ=60)
                                                                                  CGCGGGCACAGCC‑TGGGTTGGTTTCGTAGTGCAGAATACGCGAAGCATGGGCGTACAGCACTTTATCGCCCTTCACTTTCTCGAAAGAAGGCAG              <  SRR4302543.2333322/94‑1 (MQ=60)
                                                                                  CGCGGGCACAGCC‑TGGGGTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGCGTACAGCACTTTATCGCCCTTCACTTTCTCGAAAGAAGGCAG              >  SRR4302543.3985944/1‑94 (MQ=60)
                                                                                   GCGGGCACAGCC‑TGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGCGTACAGCACTTTATCGCCCTTCACTTTCTCGAAAGAAGGCAGC             <  SRR4302543.347059/94‑1 (MQ=60)
                                                                                   GCGGGCACAGCC‑TGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGCGTACAGCACTTTATCGCCCTTCACTTTCTCGAAAGAAGGCAGC             >  SRR4302543.982058/1‑94 (MQ=60)
                                                                                     GGGCACAGCC‑TGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGCGTACAGCACTTTATCGCCCTTCACTTTCTCGAAAGAAGGCAGCAA           >  SRR4302543.2322120/1‑94 (MQ=60)
                                                                                     GGGCACAGCC‑TGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGCGTACAGCACTTTATCGCCCTTCACTTTCTCGAAAGAAGGCAGCAA           >  SRR4302543.1231721/1‑94 (MQ=60)
                                                                                      GGCACAGCC‑TGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGCGTACAGCACTTTATCGCCCTTCACTTTCTCGAAAGAAGGCAGCAAC          <  SRR4302543.6329043/94‑1 (MQ=60)
                                                                                      GGCACAGCC‑TGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGCGTACAGCACTTTATCGCCCTTCACTTTCTCGAAAGAAGGCAGCAAC          <  SRR4302543.1153501/94‑1 (MQ=60)
                                                                                      GGCACAGCC‑TGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGCGTACAGCACTTTATCGCCCTTCACTTTCTCGAAAGAAGGCAGCAAC          <  SRR4302543.3658613/94‑1 (MQ=60)
                                                                                        CACAGCC‑TGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGCGTACAGCACTTTATCGCCCTTCACTTTCTCGAAAGAAGGCAGCAACAC        >  SRR4302543.4135819/1‑94 (MQ=60)
                                                                                        CACAGCC‑TGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGCGTACAGCACTTTATCGCCCTTCACTTTCTCGAAAGAAGGCAGCAACAC        >  SRR4302543.6033944/1‑94 (MQ=60)
                                                                                        CACAGCC‑TGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGCGTACAGCACTTTATCGCCCTTCACTTTCTCGAAAGAAGGCAGCAACAC        >  SRR4302543.6943875/1‑94 (MQ=60)
                                                                                        CACAGCC‑TGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGCGTACAGCACTTTATCGCCCTTCACTTTCTCGAAAGAAGGCAGCAACAC        <  SRR4302543.4880855/94‑1 (MQ=60)
                                                                                           agcC‑TGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGCGTACAGCACTTTATCGCCCTTCACTTTCTCGAAAGAAGGCAGCAACACGTA     <  SRR4302543.2222280/91‑1 (MQ=60)
                                                                                           agcC‑TGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGCGTACAGCACTTTATCGCCCTTCACTTTCTCGAAAGAAGGCAGCAACACGTA     <  SRR4302543.7419711/91‑1 (MQ=60)
                                                                                           agcC‑TGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGCGTACAGCACTTTATCGCCCTTCACTTTCTCGAAAGAAGGCAGCAACACGTA     >  SRR4302543.4265882/4‑94 (MQ=60)
                                                                                           agcC‑TGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGCGTACAGCACTTTATCGCCCTTCACTTTCTCGAAAGAAGGCAGCAACACGTA     >  SRR4302543.413501/4‑94 (MQ=60)
                                                                                            gcC‑TGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGCGTACAGCACTTTATCGCCCTTCACTTTCTCGAAAGAAGGCAGCAACACGTAG    <  SRR4302543.2904512/92‑1 (MQ=60)
                                                                                            gcC‑TGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGCGTACAGCACTTTATCGCCCTTCACTTTCTCGAAAGAAGGCAGCAACACGTAG    <  SRR4302543.6754704/92‑1 (MQ=60)
                                                                                            C‑C‑TGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGCGTACAGCACTTTATCGCCCTTCACTTTCTCGAAAGAAGGCAGCAACACGTAGG   >  SRR4302543.4924092/1‑94 (MQ=60)
                                                                                            C‑C‑TGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGCGTACAGCACTTTATCGCCCTTCACTTTCTCGAAAGAAGGCAGCAACACGTAGG   >  SRR4302543.4955679/1‑94 (MQ=60)
                                                                                              C‑TGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGCGTACAGCACTTTATCGCCCTTCACTTTCTCGAAAGAAGGCAGCAACACGTAGGT  >  SRR4302543.5625516/1‑94 (MQ=60)
                                                                                              C‑TGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGCGTACAGCACTTTATCGCCCTTCACTTTCTCGAAAGAAGGCAGCAACACGTAGGT  >  SRR4302543.1293933/1‑94 (MQ=60)
                                                                                              |                                                                                              
ACGCTGTCCATCTCTTCGGTAGAAAGCGGAATAGCAGGCGGGTTGATCCACACATAGCGGTCGCCGTGTTTTTGCATCAATGCGCGGGCACAG‑C‑TGGGTTGGTTTCGTGGTGCAGAATACGCGAAGCATGGGCGTACAGCACTTTATCGCCCTTCACTTTCTCGAAAGAAGGCAGCAACACGTAGGT  >  NC_000913/3158109‑3158295

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑