Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I2 R1	1873	183.1	9376148	98.2%	9207377	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	102,502	A→G	A90A (GCA→GCG)	ddlB →	D‑alanine‑D‑alanine ligase B

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	102,502	0	A	G	99.4%	540.2 / ‑5.8	172	A90A (GCA→GCG)	ddlB	D‑alanine‑D‑alanine ligase B
Reads supporting (aligned to +/- strand): ref base A (0/0); major base G (93/78); minor base C (1/0); total (94/78)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01

Only 100 of 179 total aligned reads displayed.

CGCGCTACACGGTCGCGGCGGTGAAGATGGTACGCTGCAGGGGATGCTCGAGCTGATGGGCTTGCCTTATACCGGAAGCGGAGTGATGGCATCTGCGCTTTCAATGGATAAACTACGCAGCAAACTTCTATGGCAAGGTGCCGGTTTACCGGTCGCGCCGTGGGTAGCGTTAACCCGCGC  >  NC_000913/102412‑102591

cgcgCTACACGGTCGCGGCGGTGAAGATGGTACGCTGCAGGGGATGCTCGAGCTGATGGGCTTGCCTTATACCGGAAGCGGAGTGATGGCGTc                                                                                         <  1:1911548/93‑1 (MQ=255)

 gcgcTACACGGTCTCGGCGGTGAAGATGGTACGCTGCAGGGGATGCTCGAGCTGATGGGCTTGCCTTATACCGGAAGCGGAGTGATGGCGTCt                                                                                        <  1:1972446/93‑1 (MQ=255)

 gcgcTACACGGTCGCGGCGGTGAAGATGGTACGCTGCAGGGGATGCTCGAGCTGATGGGCTTGCCTTATACCGGAAGCGGAGTGATGGCGTCt                                                                                        <  2:4096203/93‑1 (MQ=255)

  cgcTACACGGTCGCGGCGGTGAAGATGGTACGCTGCAGGGGATGCTCGAGCTGATGGGCTTGCCTTATACCGGAAGCGGAGTGATGGCGTCTg                                                                                       <  1:2683252/93‑1 (MQ=255)

   gcTACACGGTCGCGGCGGTGAAGGTGGTACGCTGCAGGGGATGCTCGAGCTGATGGGGTTGCCTTATACCGGAAGCGGTGGGATGGCGTCTgg                                                                                      >  2:2095564/1‑92 (MQ=255)

    cTACACGGTCGCGGCGGTGAAGATGGTACGCTGCAGGGGATGCTCGAGCTGATGGGCTTGCCTTATACCGGAAGCGGAGTGATGGCGTCTgcg                                                                                     >  1:2212500/1‑93 (MQ=255)

     tACACGGTCGCGGCGGTGAAGATGGTACGCTGCAGGGGATGCTCGAGCTGATGGGCTTGCCTTATACCGGAAGCGGAGTGATTGCGTCTgagc                                                                                    >  2:2892814/1‑93 (MQ=255)

     tACACGGTCGCGGCGGTGAAGATGGTACGCTGCAGGGGATGCTCGAGCTGATGGGCTTGCCTTATACCGGAAGCGGAGTGATGGCGTCTgcgc                                                                                    >  2:1044525/1‑93 (MQ=255)

     tACACGGTCGCGGCGGTGAAGATGGTACGCTGCAGGGGATGCTCGAGCTGATGGGCTTGCCTTATACCGGAAGCGGAGTGATGGCGTCTgcgc                                                                                    >  2:3002257/1‑93 (MQ=255)

     tACACGGTCGCGGCGGTGAAGATGGTACGCTGCAGGGGATGCTCGAGCTGATGGGCTTGCCTTATACCGGAAGCGGAGTGATGGCGTCTgcgc                                                                                    <  1:264665/93‑1 (MQ=255)

     tACACGGTCGCGGCGGTGAAGATGGTACGCTGCAGGGGATGCTCGAGCTGATGGGCTTGCCTTATACCGGAAGCGGAGTGATGGCGTCTgcgc                                                                                    >  1:939078/1‑93 (MQ=255)

     gacacGGTCGCGGCGGTGAAGATGGTACGCTGCAGGGGATGCTCGAGCTGATGGGCTTGCCTTATACCGGAAGCGGAGTGATGGCGTCTgcgc                                                                                    <  2:1848141/92‑1 (MQ=255)

      acacGGTCGCGGCGGTGAAGATGGTACGCTGCAGGGGATGCTCGAGCTGATGGGCTTGCCTTATACCGGAAGCGGAGTGATGGCGTCTGCGCt                                                                                   >  2:3111800/1‑93 (MQ=255)

       cacGGTCGCGGCGGGGAAGATGGTACGCTGCAGGGGATGCTCGAGCTGATGGGCTTGCCTTATACCGGAAGCGGAGTGATGGCGTCTGCGCtt                                                                                  >  2:1475576/1‑93 (MQ=255)

        acGGTCGCGGCGGTGAAGATGGTACGCTGCAGGGGATGCTCGAGCTGATGGGCTTGCCTTATACCGGAAGCGGAGTGATGGCGTCTGCGCttt                                                                                 >  1:560706/1‑93 (MQ=255)

         cGGTCGCGGCGGTGAAGATGGTACGCTGCAGGGGATGCTCGAGCTGATGGGCTTGCCTTATCCCGGAAGCGGAGTGATGGCGTCTGCGCTTTc                                                                                <  2:160598/93‑1 (MQ=255)

          ggTCGCGGCGGTGAAGATGGTACGCTGCAGGGGATGCTCGAGCTGATGGGCTTGCCTTATACCGGAAGCGGAGTGATGGCGTCTGCGCTTTCa                                                                               <  2:374506/93‑1 (MQ=255)

           gTCGCGGCGGTGAAGATGGTACGCTGCAGGGGATGCTCGAGCTGATGGGCTTGCCTTATACCGGAAGCGGAGTGATGGCGTCTGCGCTTTCaa                                                                              >  1:3466174/1‑93 (MQ=255)

           gTCGCGGCGGTGAAGATGGTACGCTGCAGGGGATGCTCGAGCTGATGGGCTTGCCTTATACCGGAAGCGGAGTGATGGCGTCTGCGCTTTCaa                                                                              <  2:893247/93‑1 (MQ=255)

           gTCGCGGCGGTGAAGATGGTACGCTGCAGGGGATGCTCGAGCTGATGGGCTTGCCTTATACCGGAAGCGGAGTGATGGCGTCTGCGCTTTCaa                                                                              >  1:2040782/1‑93 (MQ=255)

            tCGCGGCGTTGAAGATGGTACGCTGCAGGGGATGCTCGAGCTGATGGGCTTGCCTTATACCGGAAGCGGAGTGATGGCGCCTGCGCTTTCAAt                                                                             <  2:3814065/93‑1 (MQ=255)

            tCGCGGCGGTGAAGATGGTACGCTGCAGGGGATGCTCGAGCTGATGGGCTTGCCTTATACCGGAAGCGGAGTGATGGCGTCTGCGCTTTCAAt                                                                             <  1:275206/93‑1 (MQ=255)

            tCGCGGCGGTGAAGATGGTACGCTGCAGGGGATGCTCGAGCTGATGGGCTTGCCTTATACCGGAAGCGGAGTGATGGCGTCTGCGCTTTCAAt                                                                             <  1:4379307/93‑1 (MQ=255)

             ctcggcggTGAAGATGGTACGCTGCAGGGGATGCTCGAGCTGATGGGCTTGCCTTATACCGGAAGCGGAGTGATGGCGTCTGCGCTTTCAATg                                                                            <  2:2010004/91‑1 (MQ=255)

             cgcgGCGGTGAAGATGGTACGCTGCAGGGGATGCTCGAGCTGATGGGCTTGCCTTATACCGGAAGCGGAGTGATGGCGTCTGCGCTTTCAATg                                                                            <  2:3758800/93‑1 (MQ=255)

              gcggcgGTGAAGATGGTACGCTGCAGGGGATGCTCGAGCTGATGGGCTTGCCTTATACCGGAAGCGGAGTGATGGCGTCTGCGCTTTCAATgg                                                                           <  1:3283527/93‑1 (MQ=255)

               cggcggTGAAGATGGTACGCTGCAGGGGATGCTCGAGCTGATGGGCTTGCCTTATACCGGAAGCGGAGTGATGGCGTCTGCGCTTTCAATGGa                                                                          <  1:2491612/93‑1 (MQ=255)

               cggcggTGAAGATGGTACGCTGCAGGGGATGCTCGAGCTGATGGGCTTGCCTTATACCGGAAGCGGAGTGATGGCGTCTGCGCTTTCAATGGa                                                                          <  1:4259358/93‑1 (MQ=255)

                ggcggTGAAGATGGTACGCTGCAGGGGATGCTCGAGCTGATGGGCTTGCCTTATACCGGAAGCGGAGTGATGGCGTCTGCGCTTTCAATGGAt                                                                         <  2:4373306/93‑1 (MQ=255)

                 gcggTGAAGATGGTACGCTGCAGGGGATGCTCGAGCTGATGGGCTTGCCTTATACCGGAAGCGGAGTGATGGCGTCTGCGCTTTCAATGGATa                                                                        >  1:3780639/1‑93 (MQ=255)

                   ggTGAAGATGGTACGCTGCAGGGGATGCTCGAGCTGATGGGCTTGCCTTATACCGGAAGCGGAGTGATGGCGTCTGCGCTTTCAATGGATaaa                                                                      >  1:859788/1‑93 (MQ=255)

                    gTGAAGATGGTACGCTGCAGGGGATGCTCGAGCTGATGGGCTTGCCTTATACCGGAAGCGGAGTGATGGCGTCTGCGCTTTCAATGGATAAAc                                                                     >  2:337648/1‑93 (MQ=255)

                    gTGAAGATGGTACGCTGCAGGGGATGCTCGAGCTGATGGGCTTGCCTTATACCGGAAGCGGAGTGATGGCGTCTGCGCTTTCAATGGATAAAc                                                                     >  2:4048258/1‑93 (MQ=255)

                    gTGAAGATGGAAGGCTGCAGGGGATGCTCGAGCAGATGGGCTTGCCTTATACCGGAAGCGGAGTGATGGCGTCTGCGCTTTCAATGGCTAAAc                                                                     >  2:2344547/1‑93 (MQ=255)

                     tGAAGATGGTACGCTGCAGGGGATGCTCGAGCTGATGGGCTTGCCTTATACCGGAAGCGGAGTGATGGCGTCTGCGCTTTCAATGGATAAACt                                                                    <  1:3243361/93‑1 (MQ=255)

                      gAAGATGGTACGCTGCAGGGGATGCTCGAGCTGATGGGCTTGCCTTATACCGGAAGCGGAGTGATGGCGTCTGCGCTTTCAATGGATAAACTa                                                                   >  2:2772410/1‑93 (MQ=255)

                       aaGATGGTACGCTGCAGGGGATGCTCGAGCTGATGGGCTTGCCTTATACCGGAAGCGGAGTGATGGCGTCTGCGCTTTCAATGGATAAACTAc                                                                  >  1:4687882/1‑93 (MQ=255)

                       aaGATGGTACGCTGCAGGGGATGCTCGAGCTGATGGGCTTGCCTTATACCGGAAGCGGAGTGATGGCGTCTGCGCTTTCAATGGATAAACTAc                                                                  >  2:1929516/1‑93 (MQ=255)

                       aaGATGGTACGCTGCAGGGGATGCTCGAGCTGATGGGCTTGCCTTATACCGGAAGCGGAGTGATGGCGTCTGCGCTTTCAATGGATAAACTAc                                                                  <  1:2464498/93‑1 (MQ=255)

                        aGATGGTACGCTGCAGGGGATGCTCGAGCTGATGGGCTTGCCTTATACCGGAAGCGGAGTGATGGCGTCTGCGCTTTCAATGGATAAACTACg                                                                 >  2:4196995/1‑93 (MQ=255)

                        aGATGGTACGCTGCAGGGGATGCTCGAGCTGATGGGCTTGCCTTATACCGGAAGCGGAGTGATGGCGTCTGCGCTTTCAATGGATAAACTACg                                                                 >  1:2388971/1‑93 (MQ=255)

                         gATGGTACGCTGCAGGGGATGCTCGAGCTGATGGGCTTGCCTTATACCGGAAGCGGAGTGATGGCGTCTGCGCTTTCAATGGATAAACTACgc                                                                >  1:4311325/1‑93 (MQ=255)

                            ggTACGCTGCAGGGGATGCTCGAGCTGATGGGCTTGCCTTATACCGGAAGCGGAGTGATGGCGTCTGCGCTTTCAATGGATAAACTACgcagc                                                             >  1:3818292/1‑93 (MQ=255)

                            ggTACGCTGCAGGGGATGCTCGAGCTGATGGGCTTGCCTTATACCGGAAGCGGAGTGATGGCGTCTGCGCTTTCAATGGATAAACTACgcagc                                                             <  1:1480169/93‑1 (MQ=255)

                             gTACGCTACAGGGGATGCTCGAGCTGATGGGCTTGCCTTATACCGGAAGCGGAGTGATGGCGTCTGCGCTTTCAATGGATAAACTACgcagca                                                            >  2:1000941/1‑93 (MQ=255)

                                cGCTGCAGGGGATGCTCGAGCTGATGGGCTTGCCTTATACCGGAAGCGGAGTGATGGCGTCTGCGCTTTCAATGGATAAACTACGCAGCAAAc                                                         >  1:1097085/1‑93 (MQ=255)

                                cGCTGCAGGGGATGCTCGAGCTGATGGGCTTGCCTTATACCGGAAGCGGAGTGATGGCGTCTGCGCTTTCAATGGATAAACTACGCAGCAAAc                                                         >  2:1658689/1‑93 (MQ=255)

                                cGCTGCAGGGGATGCTCGAGCTGATGGGCTTGCCTTATACCGGAAGCGGAGTGATGGCGTCTGCGCTTTCAATGGATAAACTACGCAGCAAAc                                                         <  1:2137360/93‑1 (MQ=255)

                                 gCTGCAGGGGATGCTCGAGCTGATGGGCTTGCCTTATACCGGAAGCGGAGTGATGGCGTCTGCGCTTTCAATGGATAAACTACGCAGCAAACt                                                        >  2:293168/1‑93 (MQ=255)

                                   tGCAGGGGATGCTCGAGCTGATGGGCTTGCCTTATACCGGAAGCGGAGTGATGGCGTCTGCGCTTTCAATGGATAAACTACGCAGCAAACTTc                                                      <  1:3977007/93‑1 (MQ=255)

                                    gCAGGGGATGCTCGAGCTGATGGGCTTGCCTTATACCGGAAGCGGAGTGATGGCGTCTGCGCTTTCAATGGATAAACTACGCAGCAAACTTCt                                                     >  1:3515042/1‑93 (MQ=255)

                                    gCAGGGGATGCTCGAGCTGATGGGCTTGCCTTATACCGGAAGCGGAGTGATGGCGTCTGCGCTTTCAATGGATAAACTACGCAGCAAACTTCt                                                     <  2:4524927/93‑1 (MQ=255)

                                     cAGGGGATGCTCGAGCTGATGGGCTTGCCTTATACCGGAAGCGGAGTGATGGCGTCTGCGCTTTCAATGGATAAACTACGCAGCAAACTTCTa                                                    <  1:4529169/93‑1 (MQ=255)

                                     cAGGGGATGCTCGAGCTGATGGGCTTGCCTTATACCGGAAGCGGAGTGATGGCGTCTGCGCTTTCAATGGATAAACTACGCAGCAAACTTCTa                                                    <  2:3794698/93‑1 (MQ=255)

                                     cAGGGGATGCTCGAGCTGATGGGCTTGCCTTATACCGGAAGCGGAGTGATGGCGTCTGCGCTTTCAATGGATAAACTACGCAGCAAACTTCTa                                                    >  2:2093047/1‑93 (MQ=255)

                                      aGGGGATGCTCGAGCTGATGGGCTTGCCTTATACCGGAAGCGGAGTGATGGCGTCTGCGCTTTCAATGGATAAACTACGCAGCAAACTTCTAt                                                   >  1:4503825/1‑93 (MQ=255)

                                      aGGGGATGCTCGAGCTGATGGGCTTGCCTTATACCGGAAGCGGAGTGATGGCGTCTGCGCTTTCAATGGATAAACTACGCAGCAAACTTCTAt                                                   <  1:3496938/93‑1 (MQ=255)

                                       ggtgATGCTCGAGCTGATGGGCTTGCCGTATACCGGAAGCGGAGTGATGGCGTCTGCGCTTTCAATGGATAAACTACGCAGCAAACTTCTATg                                                  <  1:1608529/90‑1 (MQ=255)

                                        gggATGCTCGAGCTGATGGGCTTGCCTTATACCGGAAGCGGAGTGATGGCGTCTGCGCTTTCAATGGATAAACTACGCAGCAAACTTCTATgg                                                 >  1:4462753/1‑93 (MQ=255)

                                         ggATGCTCGAGCTGATGGGCTTGCCTTATACCGGAAGCGGAGTGATGGCGTCTGCGCTTTCAATGGATAAACTACGCAGCAAACTTCTATGGc                                                <  1:2028786/93‑1 (MQ=255)

                                         ggATGCTCGAGCTGATGGGCTTGCCTTATACCGGAAGCGGAGTGATGGCGTCTGCGCTTTCAATGGATAAACTACGCAGCAAACTTCTATGGc                                                >  1:4477679/1‑93 (MQ=255)

                                           aTGCTCGAGCTGATGGGCTTGCCTTATACCGGAAGCGGAGTGATGGCGTCTGCGCTTTCAATGGATAAACTACGCAGCAAACTTCTATGGCaa                                              <  2:4127344/93‑1 (MQ=255)

                                           aTGCTCGAGCTGATGGGCTTGCCTTATACCGGAAGCGGAGTGATGGCGTCTGCGCTTTCAATGGATAAACTACGCAGCAAACTTCTATGGCaa                                              <  1:1054278/93‑1 (MQ=255)

                                            tGCTCGAGCTGATGGGCTTGCCTTATACCGGAAGCGGAGTGATGGCGTCTGCGCTTTCAATGGATAAACTACGCAGCAAACTTCTATGGCAAg                                             >  2:2033200/1‑93 (MQ=255)

                                            tGCTCGAGCTGATGGGCTTGCCTTATACCGGAAGCGGAGTGATGGCGTCTGCGCTTTCAATGGATAAACTACGCAGCAAACTTCTATGGCAAg                                             >  1:812350/1‑93 (MQ=255)

                                             gCTCGAGCTGATGGGCTTGCCTTATACCGGAAGCGGAGTGATGGCGTCTGCGCTTTCAATGGATAAACTACGCAGCAAACTTCTATGGCAAgg                                            <  1:1215500/93‑1 (MQ=255)

                                               tCGAGCTGATGGGCTTGCCTTATACCGGAAGCGGAGTGATGGCGTCTGCGCTTTCAATGGATAAACTACGCAGCAAACTTCTATGGCAAGGTg                                          <  1:3137404/93‑1 (MQ=255)

                                                 gAGCTGATGGGCTTGCCTTATACCGGAAGCGGAGTGATGGCGTCTGCGCTTTCAATGGATAAACTACGCAGCAAACTTCTATGGCAAGGTGcc                                        >  1:3393160/1‑93 (MQ=255)

                                                 gAGCTGATGGGCTTGCCTTATACCGGAAGCGGAGTGATGGCGTCTGCGCTTTCAATGGATAAACTACGCAGCAAACTTCTATGGCAAGGTGcc                                        >  2:2557492/1‑93 (MQ=255)

                                                   gCTGATGGGCTTGCCTTATACCGGAAGCGGAGTGATGGCGTCTGCGCTTTCAATGGATAAACTACGCAGCAAACTTCTATGGCAAGGTGCCgg                                      >  2:414839/1‑93 (MQ=255)

                                                    cTGATGGGCTTGCCTTATACCGGAAGCGGAGTGATGGCGTCTGCGCTTTCAATGGATAAACTACGCAGCAAACTTCTATGGCAAGGTGCCGGt                                     >  1:3664389/1‑93 (MQ=255)

                                                     tGATGGGCTTGCCTTATACCGGAAGCGGAGTGATGGCGTCTGCGCTTTCAATGGATAAACTACGCAGCAAACTTCTATGGCAAGGTGCCGGtt                                    >  2:97564/1‑93 (MQ=255)

                                                     tGATGGGCTTGCCTTATACCGGAAGCGGAGTGATGGCGTCTGCGCTTTCAATGGATAAACTACGCAGCAAACTTCTATGGCAAGGTGCCGGtt                                    <  1:3947537/93‑1 (MQ=255)

                                                     tGATGGGCTTGCCTTATACCGGAAGCGGAGTGATGGCGTCTGCGCTTTCAATGGATAAACTACGCAGCAAACTTCTATGGCAAGGTGCCGGtt                                    <  2:3159802/93‑1 (MQ=255)

                                                       aTGGGCTTGCCTTATACCGGAAGCGGAGTGATGGCGTCTGCGCTTTCAATGGATAAACTACGCAGCAAACTTCTATGGCAAGGTGCCGGTTTa                                  <  2:1060290/93‑1 (MQ=255)

                                                       aTGGGCTTGCCTTATACCGGAAGCGGAGTGATGGCGTCTGCGCTTTCAATGGATAAACTACGCAGCAAACTTCTATGGCAAGGTGCCGGTTTa                                  >  1:2227913/1‑93 (MQ=255)

                                                       aTGGGCTTGCCTTATACCGGAAGCGGAGTGATGGCGTCTGCGCTTTCAATGGATAAACTACGCAGCAAACTTCTATGGCAAGGTGCCGGTTTa                                  >  2:3573313/1‑93 (MQ=255)

                                                         gggCTTGCCTTATACCGGAAGCGGAGTGATGGCGTCTGCGCTTTCAATGGATAAACTACGCAGCAAACTTCTATGGCAAGGTGCCGGTTTAcc                                <  1:1582045/93‑1 (MQ=255)

                                                             ttGCCTTATACCGGAAGCGGAGTGATGGCGTCTGCGCTTTCAATGGATAAACTACGCAGCAAACTTCTATGGCAAGGTGCCGGGTTACCGGTc                            >  1:1342407/1‑93 (MQ=255)

                                                               gCCTTATACCGGAAGCGGAGTGATGGCGTCTGCGCTTTCAATGGATAAACTACGCAGCAAACTTCTATGGCAAGGTGCCGGTTTACCGGTcgc                          >  2:1804947/1‑93 (MQ=255)

                                                                ccTTATACCGGAAGCGGAGTGATGGCGTCTGCGCTTTCAATGGATAAACTACGCAGCAAACTTCTATGGCAAGGTGCCGGTTTACCGGTcgcg                         >  2:2234708/1‑93 (MQ=255)

                                                                 cTTATACCGGAAGCGGAGTGATGGCGTCTGCGCTTTCAATGGATAAACTACGCAGCAAACTTCTATGGCAAGGTGCCGGTTTACCGGTCgcgc                        >  2:3019244/1‑93 (MQ=255)

                                                                  ttATACCGGAAGCGGAGTGATGGCGTCTGCGCTTTCAATGGATAAACTACGCAGCAAACTTCTATGGCAAGGTGCCGGTTTACCGGTCGCGcc                       >  2:1525064/1‑93 (MQ=255)

                                                                  ttATACCGGAAGCGGAGTGATGGCGTCTGCGCTTTCAATGGATAAACTACGCAGCAAACTTCTATGGCAAGGTGCCGGTTTACCGGTCGCGcc                       >  1:1726993/1‑93 (MQ=255)

                                                                   tataCCGGAAGCGGAGTGATGGCGTCTGCGCTTTCAATGGATAAACTACGCAGCAAACTTCTATGGCAAGGTGCCGGTTTACCGGTCGCGCCg                      <  2:3350348/93‑1 (MQ=255)

                                                                   tataCCGGAAGCGGAGTGATGGCGTCTGCGCTTTCAATGGATAAACTACGCAGCAAACTTCTATGGCAAGGTGCCGGTTTACCGGTCGCGCCg                      <  1:420158/93‑1 (MQ=255)

                                                                    ataCCGGAAGCGGAGTGATGGCGTCTGCGCTTTCAATGGATAAACTACGCAGCAAACTTCTATGGCAAGGTGCCGGTTTACCGGTCGCGCCGt                     <  2:3595297/93‑1 (MQ=255)

                                                                     taCCGGAAGCGGAGTGATGGCGTCTGCGCTTTCAATGGATAAACTACGCAGCAAACTTCTATGGCAAGGTGCCGGTTTACCGGTCGCGCCGTg                    >  1:3512431/1‑93 (MQ=255)

                                                                      aCCGGAAGCGGAGTGATGGCGTCTGCGCTTTCAATGGATAAACTACGCAGCAAACTTCTATGGCAAGGTGCCGGTTTACCGGTCGCGCCGTgg                   <  1:2347184/93‑1 (MQ=255)

                                                                      aCCGGAAGCGGAGTGATGGCCTCTGCGCTTTCAATGGATAAACTACGCAGCAAACTTCTATGGCAAGGTGCCGGTTTACCGGTCGCGCCGTgg                   >  2:627413/1‑93 (MQ=255)

                                                                       ccGGAAGCGGAGTGATGGCGTCTGCGCTTTCAATGGATAAACTACGCAGCAAACTTCTATGGCAAGGTGCCGGTTTACCGGTCGCGCCGTggg                  <  2:1407367/93‑1 (MQ=255)

                                                                       ccGGAAGCGGAGTGATGGCGTCTGCGCTTTCAATGGATAAACTACGCAGCAAACTTCTATGGCAAGGTGCCGGTTTACCGGTCGCGCCGTggg                  <  2:4554892/93‑1 (MQ=255)

                                                                         ggAAGCGGAGTGATGGCGTCTGCGCTTTCAATGGATAAACTACGCAGCAAACTTCTATGGCAAGGTGCCGGTTTACCGGTCGCGCCGTGGGTa                <  1:3042127/93‑1 (MQ=255)

                                                                            aGCGGAGTGATGGCGTCTGCGCTTTCAATGGATAAACTACGCAGCAAACTTCTATGGCAAGGTGCCGGTTTACCGGTCGCGCCGTGGGCAGCg             >  2:1114445/1‑93 (MQ=255)

                                                                              cGGAGTGATGGCGTCTGCGCTTTCAATGGATAAACTACGCAGCAAACTTCTATGGCAAGGTGCCGGTTTACCGGTCGCGCCGTGGGTAGCGtt           <  2:3365071/93‑1 (MQ=255)

                                                                                gAGTGATGGCGTCTGCGCTTTCAATGGATAAACTACGCAGCAAACTTCTATGGCAAGGTGCCGGTTTACCGGTCGCGCCGTGGGTAGCGTTaa         >  2:3615159/1‑93 (MQ=255)

                                                                                gAGTGATGGCGTCTGCGCTTTCAATGGATAAACTACGCAGCAAACTTCTATGGCAAGGTGCCGGTTTACCGGTCGCGCCGTGGGTAGCGTTaa         >  1:889288/1‑93 (MQ=255)

                                                                                      tGGCGTCTGCGCTTTCAATGGATAAACTACGCAGCAAACTTCTATGGCAAGGTGCCGGTTTACCGGTCGCGCCGTGGGTAGCGTTAACCcgcg   >  2:2890887/1‑93 (MQ=255)

                                                                                      tGGCGTCTGCGCTTTCAATGGATAAACTACGCAGCAAACTTCTATGGCAAGGTGCCGGTTTACCGGTCGCGCCGTGGGTAGCGTTAACCcgcg   >  2:3258734/1‑93 (MQ=255)

                                                                                      tGGCGTCTGCGCTTTCAATGGATAAACTACGCAGCAAACTTCTATGGCAAGGTGCCGGTTTACCGGTCGCGCCGTGGGTAGCGTTAACCcgcg   >  1:102913/1‑93 (MQ=255)

CGCGCTACACGGTCGCGGCGGTGAAGATGGTACGCTGCAGGGGATGCTCGAGCTGATGGGCTTGCCTTATACCGGAAGCGGAGTGATGGCATCTGCGCTTTCAATGGATAAACTACGCAGCAAACTTCTATGGCAAGGTGCCGGTTTACCGGTCGCGCCGTGGGTAGCGTTAACCCGCGC  >  NC_000913/102412‑102591

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 21 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

CGCGCTACACGGTCGCGGCGGTGAAGATGGTACGCTGCAGGGGATGCTCGAGCTGATGGGCTTGCCTTATACCGGAAGCGGAGTGATGGCATCTGCGCTTTCAATGGATAAACTACGCAGCAAACTTCTATGGCAAGGTGCCGGTTTACCGGTCGCGCCGTGGGTAGCGTTAACCCGCGCAGAG  >  NC_000913/102412‑102595

CGCGCTACACGGTCGCGGCGGTGAAGATGGTACGCTGCAGGGGATGCTCGAGCTGATGGGCTTGCCTTATACCGGAAGCGGAGTGATGGCGTCT                                                                                            <  SRR4302535.1925323/94‑1 (MQ=60)

CGCGCTACACGGTCGCGGCGGTGAAGATGGTACGCTGCAGGGGATGCTCGAGCTGATGGGCTTGCCTTATACCGGAAGCGGAGTGATGGCGTCT                                                                                            >  SRR4302535.3540195/1‑94 (MQ=60)

CGCGCTACACGGTCGCGGCGGTGAAGATGGTACGCTGCAGGGGATGCTCGAGCTGATGGGCTTGCCTTATACCGGAAGCGGAGTGATGGCGTCT                                                                                            <  SRR4302535.4392589/94‑1 (MQ=60)

 GCGCTACACGGTCTCGGCGGTGAAGATGGTACGCTGCAGGGGATGCTCGAGCTGATGGGCTTGCCTTATACCGGAAGCGGAGTGATGGCGTCTG                                                                                           <  SRR4302535.1986652/94‑1 (MQ=60)

 GCGCTACACGGTCGCGGCGGTGAAGATGGTACGCTGCAGGGGATGCTCGAGCTGATGGGCTTGCCTTATACCGGAAGCGGAGTGATGGCGTCTG                                                                                           <  SRR4302535.3980754/94‑1 (MQ=60)

  CGCTACACGGTCGCGGCGGTGAAGATGGTACGCTGCAGGGGATGCTCGAGCTGATGGGCTTGCCTTATACCGGAAGCGGAGTGATGGCGTCTGC                                                                                          <  SRR4302535.2702443/94‑1 (MQ=60)

   GCTACACGGTCGCGGCGGTGAAGATGGTACGCTGCAGGGGATGCTCGAGCTGATGGGCTTGCCTTATACCGGAAGCGGAGTGATGGCGTCTGCG                                                                                         >  SRR4302535.2228256/1‑94 (MQ=60)

    CTACACGGTCGCGGCGGTGAAGATGGTACGCTGCAGGGGATGCTCGAGCTGATGGGCTTGCCTTATACCGGAAGCGGAGTGATGGCGTCTGCGC                                                                                        >  SRR4302535.3427726/1‑94 (MQ=60)

    CTACACGGTCGCGGCGGTGAAGATGGTACGCTGCAGGGGATGCTCGAGCTGATGGGCTTGCCTTATACCGGAAGCGGAGTGATGGCGTCTGCGC                                                                                        >  SRR4302535.945730/1‑94 (MQ=60)

    CTACACGGTCGCGGCGGTGAAGATGGTACGCTGCAGGGGATGCTCGAGCTGATGGGCTTGCCTTATACCGGAAGCGGAGTGATGGCGTCTGCGC                                                                                        >  SRR4302535.2054630/1‑94 (MQ=60)

     TACACGGTCGCGGCGGTGAAGATGGTACGCTGCAGGGGATGCTCGAGCTGATGGGCTTGCCTTATACCGGAAGCGGAGTGATGGCGTCTGCGCT                                                                                       <  SRR4302535.266502/94‑1 (MQ=60)

      ACACGGTCGCGGCGGTGAAGATGGTACGCTGCAGGGGATGCTCGAGCTGATGGGCTTGCCTTATACCGGAAGCGGAGTGATGGCGTCTGCGCTT                                                                                      >  SRR4302535.3188179/1‑94 (MQ=60)

       CACGGTCGCGGCGGTGAAGATGGTACGCTGCAGGGGATGCTCGAGCTGATGGGCTTGCCTTATACCGGAAGCGGAGTGATGGCGTCTGCGCTTT                                                                                     >  SRR4302535.564628/1‑94 (MQ=60)

          GGTCGCGGCGGTGAAGATGGTACGCTGCAGGGGATGCTCGAGCTGATGGGCTTGCCTTATACCGGAAGCGGAGTGATGGCGTCTGCGCTTTCAA                                                                                  >  SRR4302535.2055480/1‑94 (MQ=60)

          GGTCGCGGCGGTGAAGATGGTACGCTGCAGGGGATGCTCGAGCTGATGGGCTTGCCTTATACCGGAAGCGGAGTGATGGCGTCTGCGCTTTCAA                                                                                  >  SRR4302535.3537908/1‑94 (MQ=60)

          GGTCGCGGCGGTGAAGATGGTACGCTGCAGGGGATGCTCGAGCTGATGGGCTTGCCTTATACCGGAAGCGGAGTGATGGCGTCTGCGCTTTCAA                                                                                  <  SRR4302535.1923899/94‑1 (MQ=60)

          GGTCGCGGCGGTGAAGATGGTACGCTGCAGGGGATGCTCGAGCTGATGGGCTTGCCTTATACCGGAAGCGGAGTGATGGCGTCTGCGCTTTCAA                                                                                  >  SRR4302535.3490259/1‑94 (MQ=60)

            TCGCGGCGGTGAAGATGGTACGCTGCAGGGGATGCTCGAGCTGATGGGCTTGCCTTATACCGGAAGCGGAGTGATGGCGTCTGCGCTTTCAATG                                                                                <  SRR4302535.4409272/94‑1 (MQ=60)

            TCGCGGCGGTGAAGATGGTACGCTGCAGGGGATGCTCGAGCTGATGGGCTTGCCTTATACCGGAAGCGGAGTGATGGCGTCTGCGCTTTCAATG                                                                                <  SRR4302535.277123/94‑1 (MQ=60)

             CGCGGCGGTGAAGATGGTACGCTGCAGGGGATGCTCGAGCTGATGGGCTTGCCTTATACCGGAAGCGGAGTGATGGCGTCTGCGCTTTCAATGG                                                                               <  SRR4302535.1608845/94‑1 (MQ=60)

             CGCGGCGGTGAAGATGGTACGCTGCAGGGGATGCTCGAGCTGATGGGCTTGCCTTATACCGGAAGCGGAGTGATGGCGTCTGCGCTTTCAATGG                                                                               >  SRR4302535.503592/1‑94 (MQ=60)

              GCGGCGGTGAAGATGGTACGCTGCAGGGGATGCTCGAGCTGATGGGCTTGCCTTATACCGGAAGCGGAGTGATGGCGTCTGCGCTTTCAATGGA                                                                              <  SRR4302535.3306333/94‑1 (MQ=60)

               CGGCGGTGAAGATGGTACGCTGCAGGGGATGCTCGAGCTGATGGGCTTGCCTTATACCGGAAGCGGAGTGATGGCGTCTGCGCTTTCAATGGAT                                                                             <  SRR4302535.2509592/94‑1 (MQ=60)

               CGGCGGTGAAGATGGTACGCTGCAGGGGATGCTCGAGCTGATGGGCTTGCCTTATACCGGAAGCGGAGTGATGGCGTCTGCGCTTTCAATGGAT                                                                             <  SRR4302535.4288532/94‑1 (MQ=60)

               CGGCGGTGAAGATGGTACGCTGCAGGGGATGCTCGAGCTGATGGGCTTGCCTTATACCGGAAGCGGAGTGATGGCGTCTGCGCTTTCAATGGAT                                                                             <  SRR4302535.4506505/94‑1 (MQ=60)

                GGCGGTGAAGATGGTACGCTGCAGGGGATGCTCGAGCTGATGGGCTTGCCTTATACCGGAAGCGGAGTGATGGCGTCTGCGCTTTCAATGGATA                                                                            >  SRR4302535.3806731/1‑94 (MQ=60)

                  CGGTGAAGATGGTACGCTGCAGGGGATGCTCGAGCTGATGGGCTTGCCTTATACCGGAAGCGGAGTGATGGCGTCTGCGCTTTCAATGGATAAA                                                                          >  SRR4302535.424067/1‑94 (MQ=60)

                  CGGTGAAGATGGTACGCTGCAGGGGATGCTCGAGCTGATGGGCTTGCCTTATACCGGAAGCGGAGTGATGGCGTCTGCGCTTTCAATGGATAAA                                                                          >  SRR4302535.865858/1‑94 (MQ=60)

                   GGTGAAGATGGTACGCTGCAGGGGATGCTCGAGCTGATGGGCTTGCCTTATACCGGAAGCGGAGTGATGGCGTCTGCGCTTTCAATGGATAAAC                                                                         >  SRR4302535.3865643/1‑94 (MQ=60)

                   GGTGAAGATGGTACGCTGCAGGGGATGCTCGAGCTGATGGGCTTGCCTTATACCGGAAGCGGAGTGATGGCGTCTGCGCTTTCAATGGATAAAC                                                                         <  SRR4302535.3310222/94‑1 (MQ=60)

                   GGTGAAGATGGTACGCTGCAGGGGATGCTCGAGCTGATGGGCTTGCCTTATACCGGAAGCGGAGTGATGGCGTCTGCGCTTTCAATGGATAAAC                                                                         >  SRR4302535.1266548/1‑94 (MQ=60)

                   GGTGAAGATGGTACGCTGCAGGGGATGCTCGAGCTGATGGGCTTGCCTTATACCGGAAGCGGAGTGATGGCGTCTGCGCTTTCAATGGATAAAC                                                                         <  SRR4302535.967139/94‑1 (MQ=60)

                     TGAAGATGGTACGCTGCAGGGGATGCTCGAGCTGATGGGCTTGCCTTATACCGGAAGCGGAGTGATGGCGTCTGCGCTTTCAATGGATAAACTA                                                                       <  SRR4302535.3265892/94‑1 (MQ=60)

                     TGAAGATGGTACGCTGCAGGGGATGCTCGAGCTGATGGGCTTGCCTTATACCGGAAGCGGAGTGATGGCGTCTGCGCTTTCAATGGATAAACTA                                                                       <  SRR4302535.2131155/94‑1 (MQ=60)

                      GAAGATGGTACGCTGCAGGGGATGCTCGAGCTGATGGGCTTGCCTTATACCGGAAGCGGAGTGATGGCGTCTGCGCTTTCAATGGATAAACTAC                                                                      <  SRR4302535.3525159/94‑1 (MQ=60)

                      GAAGATGGTACGCTGCAGGGGATGCTCGAGCTGATGGGCTTGCCTTATACCGGAAGCGGAGTGATGGCGTCTGCGCTTTCAATGGATAAACTAC                                                                      >  SRR4302535.4719740/1‑94 (MQ=60)

                      GAAGATGGTACGCTGCAGGGGATGCTCGAGCTGATGGGCTTGCCTTATACCGGAAGCGGAGTGATGGCGTCTGCGCTTTCAATGGATAAACTAC                                                                      >  SRR4302535.2755856/1‑94 (MQ=60)

                       AAGATGGTACGCTGCAGGGGATGCTCGAGCTGATGGGCTTGCCTTATACCGGAAGCGGAGTGATGGCGTCTGCGCTTTCAATGGATAAACTACG                                                                     >  SRR4302535.2406143/1‑94 (MQ=60)

                       AAGATGGTACGCTGCAGGGGATGCTCGAGCTGATGGGCTTGCCTTATACCGGAAGCGGAGTGATGGCGTCTGCGCTTTCAATGGATAAACTACG                                                                     <  SRR4302535.2482268/94‑1 (MQ=60)

                        AGATGGTACGCTGCAGGGGATGCTCGAGCTGATGGGCTTGCCTTATACCGGAAGCGGAGTGATGGCGTCTGCGCTTTCAATGGATAAACTACGC                                                                    >  SRR4302535.1084498/1‑94 (MQ=60)

                        AGATGGTACGCTGCAGGGGATGCTCGAGCTGATGGGCTTGCCTTATACCGGAAGCGGAGTGATGGCGTCTGCGCTTTCAATGGATAAACTACGC                                                                    >  SRR4302535.4340818/1‑94 (MQ=60)

                           TGGTACGCTGCAGGGGATGCTCGAGCTGATGGGCTTGCCTTATACCGGAAGCGGAGTGATGGCGTCTGCGCTTTCAATGGATAAACTACGCAGC                                                                 >  SRR4302535.3844641/1‑94 (MQ=60)

                           TGGTACGCTGCAGGGGATGCTCGAGCTGATGGGCTTGCCTTATACCGGAAGCGGAGTGATGGCGTCTGCGCTTTCAATGGATAAACTACGCAGC                                                                 >  SRR4302535.2005540/1‑94 (MQ=60)

                            GGTACGCTGCAGGGGATGCTCGAGCTGATGGGCTTGCCTTATACCGGAAGCGGAGTGATGGCGTCTGCGCTTTCAATGGATAAACTACGCAGCA                                                                <  SRR4302535.1490758/94‑1 (MQ=60)

                              TACGCTGCAGGGGATGCTCGAGCTGATGGGCTTGCCTTATACCGGAAGCGGAGTGATGGCGTCTGCGCTTTCAATGGATAAACTACGCAGCAAA                                                              >  SRR4302535.2678300/1‑94 (MQ=60)

                               ACGCTGCAGGGGATGCTCGAGCTGATGGGCTTGCCTTATACCGGAAGCGGAGTGATGGCGTCTGCGCTTTCAATGGATAAACTACGCAGCAAAC                                                             >  SRR4302535.1104897/1‑94 (MQ=60)

                                CGCTGCAGGGGATGCTCGAGCTGATGGGCTTGCCTTATACCGGAAGCGGAGTGATGGCGTCTGCGCTTTCAATGGATAAACTACGCAGCAAACT                                                            <  SRR4302535.2152753/94‑1 (MQ=60)

                                   TGCAGGGGATGCTCGAGCTGATGGGCTTGCCTTATACCGGAAGCGGAGTGATGGCGTCTGCGCTTTCAATGGATAAACTACGCAGCAAACTTCT                                                         >  SRR4302535.3539467/1‑94 (MQ=60)

                                   TGCAGGGGATGCTCGAGCTGATGGGCTTGCCTTATACCGGAAGCGGAGTGATGGCGTCTGCGCTTTCAATGGATAAACTACGCAGCAAACTTCT                                                         <  SRR4302535.4004178/94‑1 (MQ=60)

                                     CAGGGGATGCTCGAGCTGATGGGCTTGCCTTATACCGGAAGCGGAGTGATGGCGTCTGCGCTTTCAATGGATAAACTACGCAGCAAACTTCTAT                                                       >  SRR4302535.4534750/1‑94 (MQ=60)

                                     CAGGGGATGCTCGAGCTGATGGGCTTGCCTTATACCGGAAGCGGAGTGATGGCGTCTGCGCTTTCAATGGATAAACTACGCAGCAAACTTCTAT                                                       <  SRR4302535.4560180/94‑1 (MQ=60)

                                     CAGGGGATGCTCGAGCTGATGGGCTTGCCTTATACCGGAAGCGGAGTGATGGCGTCTGCGCTTTCAATGGATAAACTACGCAGCAAACTTCTAT                                                       >  SRR4302535.4573535/1‑94 (MQ=60)

                                      AGGGGATGCTCGAGCTGATGGGCTTGCCTTATACCGGAAGCGGAGTGATGGCGTCTGCGCTTTCAATGGATAAACTACGCAGCAAACTTCTATG                                                      <  SRR4302535.3521240/94‑1 (MQ=60)

                                      AGGGGATGCTCGAGCTGATGGGCTTGCCTTATACCGGAAGCGGAGTGATGGCGTCTGCGCTTTCAATGGATAAACTACGCAGCAAACTTCTATG                                                      >  SRR4302535.791596/1‑94 (MQ=60)

                                       GGTGATGCTCGAGCTGATGGGCTTGCCGTATACCGGAAGCGGAGTGATGGCGTCTGCGCTTTCAATGGATAAACTACGCAGCAAACTTCTATGG                                                     <  SRR4302535.1620129/94‑1 (MQ=60)

                                       GGGGATGCTCGAGCTGATGGGCTTGCCTTATACCGGAAGCGGAGTGATGGCGTCTGCGCTTTCAATGGATAAACTACGCAGCAAACTTCTATGG                                                     >  SRR4302535.4493360/1‑94 (MQ=60)

                                        GGGATGCTCGAGCTGATGGGCTTGCCTTATACCGGAAGCGGAGTGATGGCGTCTGCGCTTTCAATGGATAAACTACGCAGCAAACTTCTATGGC                                                    >  SRR4302535.4508423/1‑94 (MQ=60)

                                         GGATGCTCGAGCTGATGGGCTTGCCTTATACCGGAAGCGGAGTGATGGCGTCTGCGCTTTCAATGGATAAACTACGCAGCAAACTTCTATGGCA                                                   <  SRR4302535.2043411/94‑1 (MQ=60)

                                           ATGCTCGAGCTGATGGGCTTGCCTTATACCGGAAGCGGAGTGATGGCGTCTGCGCTTTCAATGGATAAACTACGCAGCAAACTTCTATGGCAAG                                                 >  SRR4302535.818046/1‑94 (MQ=60)

                                           ATGCTCGAGCTGATGGGCTTGCCTTATACCGGAAGCGGAGTGATGGCGTCTGCGCTTTCAATGGATAAACTACGCAGCAAACTTCTATGGCAAG                                                 <  SRR4302535.1061777/94‑1 (MQ=60)

                                             GCTCGAGCTGATGGGCTTGCCTTATACCGGAAGCGGAGTGATGGCGTCTGCGCTTTCAATGGATAAACTACGCAGCAAACTTCTATGGCAAGGT                                               <  SRR4302535.1224218/94‑1 (MQ=60)

                                               TCGAGCTGATGGGCTTGCCTTATACCGGAAGCGGAGTGATGGCGTCTGCGCTTTCAATGGATAAACTACGCAGCAAACTTCTATGGCAAGGTGC                                             <  SRR4302535.3159236/94‑1 (MQ=60)

                                                CGAGCTGATGGGCTTGCCTTATACCGGAAGCGGAGTGATGGCGTCTGCGCTTTCAATGGATAAACTACGCAGCAAACTTCTATGGCAAGGTGCC                                            <  SRR4302535.1734954/94‑1 (MQ=60)

                                                CGAGCTGATGGGCTTGCCTTATACCGGAAGCGGAGTGATGGCGTCTGCGCTTTCAATGGATAAACTACGCAGCAAACTTCTATGGCAAGGTGCC                                            >  SRR4302535.3416767/1‑94 (MQ=60)

                                                 GAGCTGATGGGCTTGCCTTATACCGGAAGCGGAGTGATGGCGTCTGCGCTTTCAATGGATAAACTACGCAGCAAACTTCTATGGCAAGGTGCCG                                           >  SRR4302535.88084/1‑94 (MQ=60)

                                                   GCTGATGGGCTTGCCTTATACCGGAAGCGGAGTGATGGCGTCTGCGCTTTCAATGGATAAACTACGCAGCAAACTTCTATGGCAAGGTGCCGGT                                         >  SRR4302535.3689758/1‑94 (MQ=60)

                                                   GCTGATGGGCTTGCCTTATACCGGAAGCGGAGTGATGGCGTCTGCGCTTTCAATGGATAAACTACGCAGCAAACTTCTATGGCAAGGTGCCGGT                                         <  SRR4302535.4105910/94‑1 (MQ=60)

                                                     TGATGGGCTTGCCTTATACCGGAAGCGGAGTGATGGCGTCTGCGCTTTCAATGGATAAACTACGCAGCAAACTTCTATGGCAAGGTGCCGGTTT                                       <  SRR4302535.3974489/94‑1 (MQ=60)

                                                      GATGGGCTTGCCTTATACCGGAAGCGGAGTGATGGCGTCTGCGCTTTCAATGGATAAACTACGCAGCAAACTTCTATGGCAAGGTGCCGGTTTA                                      >  SRR4302535.2243762/1‑94 (MQ=60)

                                                       ATGGGCTTGCCTTATACCGGAAGCGGAGTGATGGCGTCTGCGCTTTCAATGGATAAACTACGCAGCAAACTTCTATGGCAAGGTGCCGGTTTAC                                     >  SRR4302535.3706041/1‑94 (MQ=60)

                                                       ATGGGCTTGCCTTATACCGGAAGCGGAGTGATGGCGTCTGCGCTTTCAATGGATAAACTACGCAGCAAACTTCTATGGCAAGGTGCCGGTTTAC                                     >  SRR4302535.1137043/1‑94 (MQ=60)

                                                        TGGGCTTGCCTTATACCGGAAGCGGAGTGATGGCGTCTGCGCTTTCAATGGATAAACTACGCAGCAAACTTCTATGGCAAGGTGCCGGTTTACC                                    <  SRR4302535.2310290/94‑1 (MQ=60)

                                                         GGGCTTGCCTTATACCGGAAGCGGAGTGATGGCGTCTGCGCTTTCAATGGATAAACTACGCAGCAAACTTCTATGGCAAGGTGCCGGTTTACCG                                   <  SRR4302535.1593465/94‑1 (MQ=60)

                                                            CTTGCCTTATACCGGAAGCGGAGTGATGGCGTCTGCGCTTTCAATGGATAAACTACGCAGCAAACTTCTATGGCAAGGTGCCGGGTTACCGGTC                                >  SRR4302535.1351988/1‑94 (MQ=60)

                                                                CCTTATACCGGAAGCGGAGTGATGGCGTCTGCGCTTTCAATGGATAAACTACGCAGCAAACTTCTATGGCAAGGTGCCGGTTTACCGGTCGCGC                            >  SRR4302535.3294258/1‑94 (MQ=60)

                                                                 CTTATACCGGAAGCGGAGTGATGGCGTCTGCGCTTTCAATGGATAAACTACGCAGCAAACTTCTATGGCAAGGTGCCGGTTTACCGGTCGCGCC                           <  SRR4302535.2397850/94‑1 (MQ=60)

                                                                 CTTATACCGGAAGCGGAGTGATGGCGTCTGCGCTTTCAATGGATAAACTACGCAGCAAACTTCTATGGCAAGGTGCCGGTTTACCGGTCGCGCC                           >  SRR4302535.291463/1‑94 (MQ=60)

                                                                 CTTATACCGGAAGCGGAGTGATGGCGTCTGCGCTTTCAATGGATAAACTACGCAGCAAACTTCTATGGCAAGGTGCCGGTTTACCGGTCGCGCC                           >  SRR4302535.1739478/1‑94 (MQ=60)

                                                                   TATACCGGAAGCGGAGTGATGGCGTCTGCGCTTTCAATGGATAAACTACGCAGCAAACTTCTATGGCAAGGTGCCGGTTTACCGGTCGCGCCGT                         <  SRR4302535.423095/94‑1 (MQ=60)

                                                                    ATACCGGAAGCGGAGTGATGGCGTCTGCGCTTTCAATGGATAAACTACGCAGCAAACTTCTATGGCAAGGTGCCGGTTTACCGGTCGCGCCGTG                        >  SRR4302535.3536838/1‑94 (MQ=60)

                                                                      ACCGGAAGCGGAGTGATGGCGTCTGCGCTTTCAATGGATAAACTACGCAGCAAACTTCTATGGCAAGGTGCCGGTTTACCGGTCGCGCCGTGGG                      <  SRR4302535.2364009/94‑1 (MQ=60)

                                                                         GGAAGCGGAGTGATGGCGTCTGCGCTTTCAATGGATAAACTACGCAGCAAACTTCTATGGCAAGGTGCCGGTTTACCGGTCGCGCCGTGGGTAG                   <  SRR4302535.938104/94‑1 (MQ=60)

                                                                         GGAAGCGGAGTGATGGCGTCTGCGCTTTCAATGGATAAACTACGCAGCAAACTTCTATGGCAAGGTGCCGGTTTACCGGTCGCGCCGTGGGTAG                   <  SRR4302535.3063443/94‑1 (MQ=60)

                                                                         GGAAGCGGAGTGATGGCGTCTGCGCTTTCAATGGATAAACTACGCAGCAAACTTCTATGGCAAGGTGCCGGTTTACCGGTCGCGCCGTGGGTAG                   <  SRR4302535.2218053/94‑1 (MQ=60)

                                                                              CGGAGTGATGGCGTCTGCGCTTTCAATGGATAAACTACGCAGCAAACTTCTATGGCAAGGTGCCGGTTTACCGGTCGCGCCGTGGGTAGCGTTA              >  SRR4302535.2465/1‑94 (MQ=60)

                                                                               GGAGTGATGGCGTCTGCGCTTTCAATGGATAAACTACGCAGCAAACTTCTATGGCAAGGTGCCGGTTTACCGGTCGCGCCGTGGGTAGCGTTAA             >  SRR4302535.895590/1‑94 (MQ=60)

                                                                                 AGTGATGGCGTCTGCGCTTTCAATGGATAAACTACGCAGCAAACTTCTATGGCAAGGTGCCGGTTTACCGGTCGCGCCGTGGGTAGCGTTAACC           >  SRR4302535.2485155/1‑94 (MQ=60)

                                                                                  GTGATGGCGTCTGCGCTTTCAATGGATAAACTACGCAGCAAACTTCTATGGCAAGGTGCCGGTTTACCGGTCGCGCCGTGGGTAGCGTTAACCC          <  SRR4302535.4547405/94‑1 (MQ=60)

                                                                                     ATGGCGTCTGCGCTTTCAATGGATAAACTACGCAGCAAACTTCTATGGCAAGGTGCCGGTTTACCGGTCGCGCCGTGGGTAGCGTTAACCCGCG       >  SRR4302535.103641/1‑94 (MQ=60)

                                                                                     ATGGCGTCTGCGCTTTCAATGGATAAACTACGCAGCAAACTTCTATGGCAAGGTGCCGGTTTACCGGTCGCGCCGTGGGTAGCGTTAACCCGCG       >  SRR4302535.442056/1‑94 (MQ=60)

                                                                                      TGGCGTCTGCGCTTTCAATGGATAAACTACGCAGCAAACTTCTATGGCAAGGTGCCGGTTTACCGGTCGCGCCGTGGGTAGCGTTAACCCGCGC      >  SRR4302535.1571930/1‑94 (MQ=60)

                                                                                       GGCGTCTGCGCTTTCAATGGATAAACTACGCAGCAAACTTCTATGGCAAGGTGCCGGTTTACCGGTCGCGCCGTGGGTAGCGTTAACCCGCGCA     >  SRR4302535.2393862/1‑94 (MQ=60)

                                                                                        GCGTCTGCGCTTTCAATGGATAAACTACGCAGCAAACTTCTATGGCAAGGTGCCGGTTTACCGGTCGCGCCGTGGGTAGCGTTAACCCGCGCAG    <  SRR4302535.819948/94‑1 (MQ=60)

                                                                                         CGTCTGCGCTTTCAATGGATAAACTACGCAGCAAACTTCTATGGCAAGGTGCCGGTTTACCGGTCGCGCCGTGGGTAGCGTTAACCCGCGCAGG   >  SRR4302535.166040/1‑94 (MQ=60)

                                                                                         CGTCTGCGCTTTCAATGGATAAACTACGCAGCAAACTTCTATGGCAAGGTGCCGGTTTACCGGTCGCGCCGTGGGTAGCGTTAACCCGCGCAGA   >  SRR4302535.1857350/1‑94 (MQ=60)

                                                                                         CGTCTGCGCTTTCAATGGATAAACTACGCAGCAAACTTCTATGGCAAGGTGCCGGTTTACCGGTCGCGCCGTGGGTAGCGTTAACCCGCGCAGA   >  SRR4302535.1093012/1‑94 (MQ=60)

                                                                                         CGTCTGCGCTTTCAATGGATAAACTACGCAGCAAACTTCTATGGCAAGGTGCCGGTTTACCGGTCGCGCCGTGGGTAGCGTTAACCCGCGCAGA   >  SRR4302535.2708422/1‑94 (MQ=60)

                                                                                          GTCTGCGCTTTCAATGGATAAACTACGCAGCAAACTTCTATGGCAAGGTGCCGGTTTACCGGTCGCGCCGTGGGTAGCGTTAACCCGCGCAGAG  <  SRR4302535.3609482/94‑1 (MQ=60)

CGCGCTACACGGTCGCGGCGGTGAAGATGGTACGCTGCAGGGGATGCTCGAGCTGATGGGCTTGCCTTATACCGGAAGCGGAGTGATGGCATCTGCGCTTTCAATGGATAAACTACGCAGCAAACTTCTATGGCAAGGTGCCGGTTTACCGGTCGCGCCGTGGGTAGCGTTAACCCGCGCAGAG  >  NC_000913/102412‑102595

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 18 ≤` `ATCG/ATCG` `< 28 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`