Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 1873 183.1 9376148 98.2% 9207377 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,980,940 +C coding (1/363 nt)
coding (627/627 nt)		 araH ←
araH ←		 ABC‑type arabinose transport system permease component
ABC‑type arabinose transport system permease component

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,980,9391.C99.3% 418.8 / ‑3.9 137coding (2/363 nt)araHABC‑type arabinose transport system permease component
Reads supporting (aligned to +/- strand):  ref base . (0/1);  new base C (68/68);  total (68/69)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.97e-01

Only 100 of 150 total aligned reads displayed.

CAGAATAATTCCGGCTATCGCTGATACCAGGCCTGAGAGAACAAAGATAATAATTTTGGTGCGAACAACCGGTACACCCGCCAGACGCGC‑GG‑C‑TCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCAAAGGTGGTTTTATTCAGCAGCAAACCAAAGATAATCAGACACGCGACGG  >  NC_000913/1980848‑1981025
                                                                                             |                                                                                       
cAGAATAATTCCGGCTATCGCTGATACCAGGCCTGAGAGAACAAAGATAATAATTTTGGTGCGAACAACCGGTACACCCGCCAGACGCGC‑GG‑c                                                                                        <  1:4074568/93‑1 (MQ=255)
    ataatTCCGGCTATCGCTGATACCAGGCCTGAGAGAACAAAGATAATAATTTTGGTGCGAACAACCGGTACACCCGCCAGACGCGC‑GG‑cctct                                                                                    >  1:2580021/1‑89 (MQ=255)
    ataatTCCGGCTATCGCTGATACCAGGCCTGAGAGAACAAAGATAATAATTTTGGTGCGAACAACCGGTACACCCGCCAGACGCGC‑GG‑cctct                                                                                    <  2:881895/93‑5 (MQ=255)
         tCCGGCTATCGCTGATACCAGGCCTGAGAGAACAAAGATAATAATTTTGGTGCGAACAACCGGTACACCCGCCAGACGCGC‑GGCC‑TCTTCGtt                                                                               >  1:3738743/1‑93 (MQ=255)
           cgtcTATCGCAGATACCAGGCCTGAGAGAACAAAGATAATAATTTTGGTGCGAACAACCGGTACACCCCCCAGACGCGCTGC‑C‑TCTTCGTTcc                                                                             <  2:736155/90‑1 (MQ=255)
           cGGCTATCGCTGATACCAGGCCTGAGAGAACAAAGATAATAATTTTGGTGCGAACAACCGGTACACCCGCCAGACGCGC‑GGCC‑TCTTCGTTcc                                                                             >  2:418619/1‑93 (MQ=255)
           cGGCTATCGCTGATACCAGGCCTGAGAGAACAAAGATAATAATTTTGGTGCGAACAACCGGTACACCCGCCAGACGCGC‑GGCC‑TCTTCGTTcc                                                                             <  2:2781526/93‑1 (MQ=255)
              cTATCGCTGATACCAGGCCTGAGAGAACAAAGATAATAATTTTGGTGCGAACAACCGGTACACCCGCCAGACGCGC‑GGCC‑TCTTCGTTCCCGc                                                                          >  2:3402516/1‑93 (MQ=255)
               tATCGCTGATACCAGGCCTGAGAGAACAAAGATAATAATTTTGGTGCGAACAACCGGTACACCCGCCAGACGCGC‑GGCC‑TCTTCGTTCCCGcc                                                                         >  1:3068568/1‑93 (MQ=255)
               tATCGCTGATACCAGGCCTGAGAGAACAAAGATAATAATTTTGGTGCGAACAACCGGTACACCCGCCAGACGCGC‑GGCC‑TCTTCGTTCCCGcc                                                                         >  1:2929245/1‑93 (MQ=255)
               tATCGCTGATACCAGGCCTGAGAGAACAAAGATAATAATTTTGGTGCGAACAACCGGTACACCCGCCAGACGCGC‑GGCC‑TCTTCGTTCCCGcc                                                                         >  1:3742499/1‑93 (MQ=255)
               tATCGCTGATACCAGGCCTGAGAGAACAAAGATAATAATTTTGGTGCGAACAACCGGTACACCCGCCAGACGCGC‑GGCC‑TCTTCGTTCCCGcc                                                                         >  2:4472196/1‑93 (MQ=255)
                 tCGCTGATACCAGGCCTGAGAGAACAAAGATAATAATTTTGGTGCGAACAACCGGTACACCCGCCAGACGCGC‑GGCC‑TCTTCGTTCCCGCCaa                                                                       <  1:3342360/93‑1 (MQ=255)
                 tCGCTGATACCAGGCCTGAGAGAACAAAGATAATAATTTTGGTGCGAACAACCGGTACACCCGCCAGACGCGC‑GGCC‑TCTTCGTTCCCGCCaa                                                                       >  1:2332326/1‑93 (MQ=255)
                  cGCTGATACCAGGCCTGAGAGAACAAAGATAATAATTTTGGTGCGAACAACCGGTACCCCCGCCAGACGCGC‑GGCC‑TCTTCGTTCCCGCCAAt                                                                      <  1:1165157/93‑1 (MQ=255)
                    cTGATACCAGGCCTGAGAGAACAAAGATAATAATTTTGGTGCGAACAACCGGTACACCCGCCAGACGCGC‑GGCC‑TCTTCGTTCCCGCCAATCg                                                                    >  1:1443310/1‑93 (MQ=255)
                    cTGATACCAGGCCTGAGAGAACAAAGATAATAATTTTGGTGCGAACAACCGGTACACCCGCCAGACGCGC‑GGCC‑TCTTCGTTCCCGCCAATCg                                                                    <  2:4313387/93‑1 (MQ=255)
                    cTGATACCAGGCCTGAGAGAACAAAGATAATAATTTTGGTGCGAACAACCGGTACACCCGCCAGACGCGC‑GGCC‑TCTTCGTTCCCGCCAATCg                                                                    >  2:3973603/1‑93 (MQ=255)
                     tGATACCAGGCCTGAGAGAACAAAGATAATAATTTTGGTGCGAACAACCGGTACACCCGCCAGACGCGC‑GGCC‑TCTTCGTTCCCGCCAATCGc                                                                   >  1:3890444/1‑93 (MQ=255)
                     tGATACCAGGCCTGAGAGAACAAAGATAATAATTTTGGTGCGAACAACCGGTACACCCGCCAGACGCGC‑GGCC‑TCTTCGTTCCCGCCAATCGc                                                                   >  1:1997181/1‑93 (MQ=255)
                      gATACCAGGCCTGAGAGAACAAAGATAATAATTTTGGTGCGAACAACCGGTACACCCGCCAGACGCGC‑GGCC‑TCTTCGTTCCCGCCAATCGcc                                                                  <  1:3199007/93‑1 (MQ=255)
                      gATACCAGGCCTGAGAGAACAAAGATAATAATTTTGGTGCGAACAACCGGTACACCCGCCAGACGCGC‑GGCC‑TCTTCGTTCCCGCCAATCGcc                                                                  <  1:2419647/93‑1 (MQ=255)
                      gATACCAGGCCTGAGAGAACAAAGATAATAATTTTGGTGCGAACAACCGGTACACCCGCCAGACGCGC‑GGCC‑TCTTCGTTCCCGCCAATCGcc                                                                  >  2:909935/1‑93 (MQ=255)
                       gtACCCGGCCTGAGAGAACAAACATAATAATTTTGGTGCGAACAACCGGTACCCCCGCCAGACGCGC‑GGCC‑TCTTCGTTCCCGCCAATCGCCa                                                                 <  1:893369/92‑1 (MQ=255)
                       aTACCAGGCCTGAGAGAACAAAGATAATAATTTTGGTGCGAACAACCGGTACACCCGCCAGACGCGC‑GGCC‑TCTTCGTTCCCGCCAATCGCCa                                                                 <  1:3478217/93‑1 (MQ=255)
                       aTACCAGGCCTGAGAGAACAAAGATAATAATTTTGGTGCGAACAACCGGTACACCCGCCAGACGCGC‑GGCC‑TCTTCGTTCCCGCCAATCGCCa                                                                 <  1:2967231/93‑1 (MQ=255)
                        tACCAGGCCTGAGAGAACAAAGATAATAATTTTGGTGCGAACAACCGGTACACCCGCCAGACGCGC‑GGCC‑TCTTCGTTCCCGCCAATCGCCAg                                                                <  1:4654343/93‑1 (MQ=255)
                        tACCAGGCCTGAGAGAACAAAGATAATAATTTTGGTGCGAACAACCGGTACACCCGCCAGACGCGC‑GGCC‑TCTTCGTTCCCGCCAATCGCCAg                                                                >  2:2172006/1‑93 (MQ=255)
                           cAGGCCTGAGAGAACAAAGATAATAATTTTGGTGCGAACAACCGGTACACCCGCCAGACGCGC‑GGCC‑TCTTCGTTCCCGCCAATCGCCAGGgt                                                             <  2:470330/93‑1 (MQ=255)
                            aGGCCTGAGAGAACAAAGATAATAATTTTGGTGCGAACAACCGGTACACCCGCCAGACGCGC‑GGCC‑TCTTCGTTCCCGCCAATCGCCAGGgtg                                                            <  1:1001250/93‑1 (MQ=255)
                               ccTGAGAGAACAAAGATAATAATTTTGGTGCGAACAAACGGTACACCCGCCAGACGCGC‑GGCC‑TCTTCGTTCCCGCCAATCGCCAGGGTGTTa                                                         >  2:1618499/1‑93 (MQ=255)
                                cTGAGAGAACAAAGATAATAATTTTGGTGCGAACAACCGGTACACCCGCCAGACGCGC‑GGCC‑TCTTCGTTCCCGCCAATCGCCAGGGTGTTAc                                                        >  2:3731351/1‑93 (MQ=255)
                                cTGAGAGAACAAAGATAATAATTTTGGTGCGAACAACCGGTACACCCGCCAGACGCGC‑GGCC‑TCTTCGTTCCCGCCAATCGCCAGGGTGTTAc                                                        >  2:3030929/1‑93 (MQ=255)
                                 tGAGAGAACAAAGATAATACTTTTGGTGCGAACAACCGGTACACCCGCCAGACGCGC‑GGCC‑TCTTCGTTCCCGCCAATCGCCAGGGTGTTACg                                                       >  2:4568657/1‑93 (MQ=255)
                                  gagagaACAAAGATAATAATTTTGGTGCGAACAACCGGTACACCCGCCAGACGCGC‑GGCC‑TCTTCGTTCCCGCCAATCGCCAGGGTGTTACGa                                                      <  1:2753146/93‑1 (MQ=255)
                                   agagaACAAAGATAATAATTTGGGTGCGAACAACCGGTACCCCCGCCAGACGCGC‑GGCC‑TCTTCGTTCCCGCCAATCGCCAGGGTGTTACGAc                                                     <  1:146342/93‑1 (MQ=255)
                                    gagaACAAAGATAATAATTTTGGTGCGAACAACCGGTACACCCGCCAGACGCGC‑GGCC‑TCTTCGTTCCCGCCAATCGCCAGGGTGTTACGAcc                                                    <  2:2381058/93‑1 (MQ=255)
                                     agaACAAAGATAATAATTTTGGTGCGAACAACCGGTACACCCGCCAGACGCGC‑GGCC‑TCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCa                                                   >  1:3667853/1‑93 (MQ=255)
                                      gaACAAAGATAATAATTTTGGTGCGAACAACCGGTACACCCGCCAGACGCGC‑GGCC‑TCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCaa                                                  >  1:3956296/1‑93 (MQ=255)
                                      gaACAAAGATAATAATTTTGGTGCGAACAACCGGTACACCCGCCAGACGCGC‑GGCC‑TCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCaa                                                  >  2:2627057/1‑93 (MQ=255)
                                       aaCAAAGATAATAATTTTGGTGCGAACAACCGGTACACCCGCCAGACGCGC‑GGCC‑TCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCaaa                                                 >  2:1802664/1‑93 (MQ=255)
                                        aCAAAGATAATAATTTTGGTGCGAACAACCGGTACACCCGCCAGACGCGC‑GGCC‑TCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCAAAg                                                <  2:1214132/93‑1 (MQ=255)
                                         cAAAGATAATAATTTTGGTGCGAACAACCGGTACACCCGCCAGACGCGC‑GGCC‑TCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCAAAgg                                               <  1:2766697/93‑1 (MQ=255)
                                          aaaGATAATAATTTTGGTGCGAACAACCGGTACACCCGCCAGACGCGC‑GGCC‑TCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCAAAggt                                              >  1:1570020/1‑93 (MQ=255)
                                           aaGATAATAATTTTGGTGCGAACAACCGGTACACCCGCCAGACGCGC‑GGCC‑TCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCAAAggtg                                             >  1:3609064/1‑93 (MQ=255)
                                            aGATAATAATTTTGGTGCGAACAACCGTGACACCCGCCAGACGCGC‑GGCC‑TCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCAAAggtgg                                            <  1:1745663/93‑1 (MQ=255)
                                            aGATAATAATTTTGGTGCGAACAACCGGTACACCCGCCAGACGCGC‑GGCC‑TCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCAAAggtgg                                            <  2:4212087/93‑1 (MQ=255)
                                             aataataATTTTGGTGCGAACACCCGGTACACCCGCCAGACGCGC‑GGCC‑TCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCAAAggtggt                                           <  2:4178037/92‑1 (MQ=255)
                                              ataataATTTTGGTGCGAACAACCGGTACACCCGCCAGACGCGC‑GGCC‑TCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCAAAGGTGGtt                                          <  1:4143439/93‑1 (MQ=255)
                                              ataataATTTTGGTGCGAACAACCGGTACACCCGCCAGACGCGC‑GGCC‑TCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCAAAGGTGGtt                                          >  1:2460969/1‑93 (MQ=255)
                                              ataataATTTTGGTGCGAACAACCGGTACACCCGCCAGACGCGC‑GGCC‑TCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCAAAGGTGGtt                                          <  1:337186/93‑1 (MQ=255)
                                               taataaTTTTGGTGCGAACAACCGGTACACCCGCCAGACGCGC‑GGCC‑TCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCAAAGGTGGttt                                         >  2:1319850/1‑93 (MQ=255)
                                               taataaTTTTGGTGCGAACAACCGGTACACCCGCCAGACGCGC‑GGCC‑TCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCAAAGGTGGttt                                         >  2:2339942/1‑93 (MQ=255)
                                                 ataatTTTGGTGCGAACAACCGGTACACCCGCCAGACGCGC‑GGCC‑TCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCAAAGGTGGTTTTa                                       >  1:2669163/1‑93 (MQ=255)
                                                 ataatTTTGGTGCGAACAACCGGTACACCCGCCAGACGCGC‑GGCC‑TCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCAAAGGTGGTTTTa                                       <  2:2273541/93‑1 (MQ=255)
                                                  taatTTTGGTGCGAACAACCGGTACACCCGCCAGACGCGC‑GGCC‑TCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCAAAGGTGGTTTTAt                                      <  1:432059/93‑1 (MQ=255)
                                                   aatTTTGGTGCGAACAACCGGTACACCCGCCAGACGCGC‑GGCC‑TCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCAAAGGTGGTTTTAtt                                     <  1:2800602/93‑1 (MQ=255)
                                                    atTTTGGTGCGAACAACCGGTACACCCGCCAGACGCGC‑GGCC‑TCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCAAAGGTGGTTTTATTc                                    >  1:559924/1‑93 (MQ=255)
                                                    atTTTGGTGCGAACAACCGGTACACCCGCCAGACGCGC‑GGCC‑TCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCAAAGGTGGTTTTATTc                                    <  2:1203014/93‑1 (MQ=255)
                                                     ttttGGTGCGAACAACCGGTACACCCGCCAGACGCGC‑GGCC‑TCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCAAAGGTGGTTTTATTca                                   >  2:925570/1‑93 (MQ=255)
                                                      tttGGTGCGAACAACCGGTACACCCGCCAGACGCGC‑GGCC‑TCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCAAAGGTGGTTTTATTcag                                  <  2:2866214/93‑1 (MQ=255)
                                                       ttGGTGCGAACAACCGGTACACCCGCCAGACGCGC‑GG‑CATCTTCGTTCCCGCCAATCGCCAGGGTGTTTCGACCAAAGGTGGTCTTATTcagc                                 >  2:2819013/1‑93 (MQ=255)
                                                        tGGTGCGAACAACCGGTACCCCCGCCAGACGCGC‑GGCC‑TCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCAAAGGTGGTTTTATTcagca                                <  1:1153988/93‑1 (MQ=255)
                                                        tGGTGCGAACAACCGGTACCCCCGCCAGACGCGC‑GGCC‑TCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCAAAGGTGGTTTTATTcagca                                <  2:185959/93‑1 (MQ=255)
                                                        tGGTGCGAACAACCGGTACACCCGCCAGACGCGC‑GGCC‑TCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCAAAGGTGGTTTTATTcagca                                <  2:4069952/93‑1 (MQ=255)
                                                        tGGTGCGAACAACCGGTACACCCGCCAGACGCGC‑GGCC‑TCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCAAAGGTGGTTTTATTcagca                                >  1:3893974/1‑93 (MQ=255)
                                                        tGGTGCGAACAACCGGTACACCCGCCAGACGCGC‑GGCC‑TCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCAAAGGTGGTTTTATTcagca                                <  2:3587891/93‑1 (MQ=255)
                                                         ggTGCGAACAACCGGTACACCCGCCAGACGCGC‑GGCC‑TCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCAAAGGTGGTTTTATTcagcag                               >  1:1020281/1‑93 (MQ=255)
                                                           tGCGAACAACCGGTACACCCGCCAGACGCGC‑GGCC‑TCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCAAAGGTGGTTTTATTCAgcagca                             <  2:3536231/93‑1 (MQ=255)
                                                            tcGAACAACCGGTACCCCCGCCAGACGCGC‑GGCC‑TCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCAAAGGTGGTTTTATTCAGCAGCaa                            <  2:2240275/92‑1 (MQ=255)
                                                             cGAACAACCGGTACCCCCGCCAGCCGCGC‑GGCC‑TCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCAAAGGTGGTTTTATTCAGCAGCaaa                           <  2:3797119/93‑1 (MQ=255)
                                                              gAACAACCGGTACACCCGCCAGACGCGC‑GGCC‑TCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCAAAGGTGGTTTTATTCAGCAGCAAAc                          >  1:1602422/1‑93 (MQ=255)
                                                              gAACAACCGGTACACCCGCCAGACGCGC‑GGCC‑TCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCAAAGGTGGTTTTATTCAGCAGCAAAc                          <  1:1790092/93‑1 (MQ=255)
                                                              gAACAACCGGTACACCCGCCAGACGCGC‑GGCC‑TCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCAAAGGTGGTTTTATTCAGCAGCAAAc                          >  1:3674396/1‑93 (MQ=255)
                                                               aacaacCGGTACACCCGCCAGACGCGC‑GGCC‑TCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCAAAGGTGGTTTTATTCAGCAGCAAAcc                         <  1:4204482/93‑1 (MQ=255)
                                                                  acccGGTCCCCCCGCCAGACGCGC‑GGCC‑TCTTCGTGCCCGCCAATCGCCAGGGTGTTACGACCAAAGGTGGTTTTATTCAGCAGCAAACCaaa                      <  2:1696535/91‑1 (MQ=255)
                                                                  aacCGGTCCACCCGCCACACGCGC‑GGCC‑TCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCAAAGGTGGTTTTATTCAGCAGCAAACCaaa                      <  2:2392108/93‑1 (MQ=255)
                                                                  aacCGGTACACCCGCCAGACGCGC‑GGCC‑TCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCAAAGGTGGTTTTATTCAGCAGCAAACCaaa                      <  2:2100036/93‑1 (MQ=255)
                                                                   acCGGTACACCCGCCAGACGCGC‑GGCC‑TCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCAAAGGTGGTTTTATTCAGCAGCAAACCAAAg                     >  2:3753955/1‑93 (MQ=255)
                                                                      ggTCCACCCGGCAGACGCGC‑GGCC‑TTTTGGTTCCCGCGAATCGCCAGGGTGTTACGACCAAAGGTGGTTTTATTCAGCAGCAAACCAAAGATa                  <  1:2466181/93‑1 (MQ=255)
                                                                      ggTACACCCGCCAGACGCGC‑GGCC‑TCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCAAAGGTGGTTTTATTCAGCAGCAAACCAAAGATa                  <  1:3170424/93‑1 (MQ=255)
                                                                       gTACACCCGCCAGACGCGC‑GGCC‑TCTTCGTTCCCGCCAATCGACAGGGTGTTACGACCAAAGGTGGTTTTATTCAGCAGCAAACCAAAGATaa                 >  1:2516486/1‑93 (MQ=255)
                                                                        tACACCCGCCAGACGCGC‑GGCC‑TCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCAAAGGTGGTTTTATTCAGCAGCAAACCAAAGATAAt                <  1:3957526/93‑1 (MQ=255)
                                                                         acacCCGCCAGACGCGC‑GGCC‑TCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCAAAGGTGGTTTTATTCAGCAGCAAACCAAAGATAATc               <  2:4529834/93‑1 (MQ=255)
                                                                          cacCCGCCAGACGCGC‑GGCC‑TCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCAAAGGTGGTTTTATTCAGCAGCAAACCAAAGATAATCa              <  1:3070484/93‑1 (MQ=255)
                                                                          cacCCGCCAGACGCGC‑GGCC‑TCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCAAAGGTGGTTTTATTCAGCAGCAAACCAAAGATAATCa              <  2:1700393/93‑1 (MQ=255)
                                                                          cacCCCCCAGACGCGC‑GGCC‑TCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCAAAGGTGGTTTTATTCAGCAGCAAACCAAAGATAATCa              <  2:145234/93‑1 (MQ=255)
                                                                             ccGCCAGACGCGC‑GGCC‑TCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCAAAGGTGGTTTTATTCAGCAGCAAACCAAAGATAATCAGac           <  2:2408089/93‑1 (MQ=255)
                                                                             ccGCCAGACGCGC‑GGCC‑TCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCAAAGGTGGTTTTATTCAGCAGCAAACCAAAGATAATCAGac           >  1:1519490/1‑93 (MQ=255)
                                                                             ccGCCAGACGCGC‑GGCC‑TCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCAAAGGTGGCTTTATTCAGCAGCAAACCAAAGATAATCCGac           >  2:48102/1‑93 (MQ=255)
                                                                              cGCCAGACGCGC‑GGCC‑TCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCAAAGGTGGTTTTATTCAGCAGCAAACCAAAGATAATCAGaca          <  2:1574984/93‑1 (MQ=255)
                                                                                 cAGACGCGC‑GGCC‑TCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCAAAGGTGGTTTTATTCAGCAGCAAACCAAAGATAATCAGACAcgc       <  1:2126823/93‑1 (MQ=255)
                                                                                 cAGACGCGC‑GGCC‑TCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCAAAGGTGGTTTTATTCAGCAGCAAACCAAAGATAATCAGACAcgc       >  1:4551195/1‑93 (MQ=255)
                                                                                  aGACGCGC‑GGCC‑TCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCAAAGGTGGTTTTATTCAGCAGCAAACCAAAGATAATCAGACAcgcg      >  1:4085326/1‑93 (MQ=255)
                                                                                    aCGCGC‑GGCC‑TCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCAAAGGTGGTTTTATTCAGCAGCAAACCAAAGATAATCAGACACGCGAc    <  2:2066806/93‑1 (MQ=255)
                                                                                    aCGCGC‑GGCC‑TCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCAAAGGTGGTTTTATTCAGCAGCAAACCAAAGATAATCAGACACGCGAc    <  2:1158274/93‑1 (MQ=255)
                                                                                    aCGCGC‑GGCC‑TCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCAAAGGTGGTTTTATTCAGCAGCAAACCAAAGATAATCAGACACGCGAc    <  1:1375814/93‑1 (MQ=255)
                                                                                     cgcgc‑gGCC‑TCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCAAAGGTGGTTTTATTCAGCAGCAAACCAAAGATAATCAGACACGCGACg   >  2:2664093/1‑93 (MQ=255)
                                                                                      gcgc‑gGCC‑TCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCAAAGGTGGTTTTATTCAGCAGCAAACCAAAGATAATCAGACACGCGACgg  >  2:4467742/1‑93 (MQ=255)
                                                                                      gcgc‑gGCC‑TCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCAAAGGTGGTTTTATTCAGCAGCAAACCAAAGATAATCAGACACGCGACgg  >  2:1842048/1‑93 (MQ=255)
                                                                                             |                                                                                       
CAGAATAATTCCGGCTATCGCTGATACCAGGCCTGAGAGAACAAAGATAATAATTTTGGTGCGAACAACCGGTACACCCGCCAGACGCGC‑GG‑C‑TCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCAAAGGTGGTTTTATTCAGCAGCAAACCAAAGATAATCAGACACGCGACGG  >  NC_000913/1980848‑1981025

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CAGAATAATTCCGGCTATCGCTGATACCAGGCCTGAGAGAACAAAGATAATAATTTTGGTGCGAACAACCGGTACACCCGCCAGACGCGCGG‑CTCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCAAAGGTGGTTTTATTCAGCAGCAAACCAAAGATAATCAGACACGCGACGGTGAGCCAG  >  NC_000913/1980848‑1981033
                                                                                             |                                                                                             
CAGAATAATTCCGGCTATCGCTGATACCAGGCCTGAGAGAACAAAGATAATAATTTTGGTGCGAACAACCGGTACACCCGCCAGACGCGCGG‑CC                                                                                              <  SRR4302535.4102445/94‑1 (MQ=60)
 AGAATAATTCCGGCTATCGCTGATACCAGGCCTGAGAGAACAAAGATAATAATTTTGGTGCGAACAACCGGTACACCCGCCAGACGCGCGG‑Cct                                                                                             >  SRR4302535.2786328/1‑92 (MQ=60)
   AATAATTCCGGCTATCGCTGATACCAGGCCTGAGAGAACAAAGATAATAATTTTGGTGCGAACAACCGGTACACCCGCCAGACGCGCGGCCTCT                                                                                            >  SRR4302535.2598555/1‑94 (MQ=60)
    ATAATTCCGTCTATCGCTGATACCAGGCCTGAGAGAACAAAGATAATAATTTTGGTGCGAACAACCGGTACACCCGCCAGACGCGCGGCCTCTT                                                                                           <  SRR4302535.1550879/94‑1 (MQ=60)
     TAATTCCGGCTATCGCTGATACCAGGCCTGAGAGAACAAAGATAATAATTTTGGTGCGAACAACCGGTACACCCGCCAGACGCGCGGCCTCTTC                                                                                          <  SRR4302535.4232159/94‑1 (MQ=60)
     TAATTCCGGCTATCGCTGATACCAGGCCTGAGAGAACAAAGATAATAATTTTGGTGCGAACAACCGGTACACCCGCCAGACGCGCGGCCTCTTC                                                                                          <  SRR4302535.3116119/94‑1 (MQ=60)
        TTCCGGCTATCGCTGATACCAGGCCTGAGAGAACAAAGATAATAATTTTGGTGCGAACAACCGGTACACCCGCCAGACGCGCGGCCTCTTCGTT                                                                                       >  SRR4302535.3764619/1‑94 (MQ=60)
              CTATCGCTGATACCAGGCCTGAGAGAACAAAGATAATAATTTTGGTGCGAACAACCGGTACACCCGCCAGACGCGCGGCCTCTTCGTTCCCGCC                                                                                 >  SRR4302535.3768394/1‑94 (MQ=60)
              CTATCGCTGATACCAGGCCTGAGAGAACAAAGATAATAATTTTGGTGCGAACAACCGGTACACCCGCCAGACGCGCGGCCTCTTCGTTCCCGCC                                                                                 >  SRR4302535.3090003/1‑94 (MQ=60)
              CTATCGCTGATACCAGGCCTGAGAGAACAAAGATAATAATTTTGGTGCGAACAACCGGTACACCCGCCAGACGCGCGGCCTCTTCGTTCCCGCC                                                                                 >  SRR4302535.2949884/1‑94 (MQ=60)
              CTATCGCTGATACCAGGCCTGAGAGAACAAAGATAATAATTTTGGTGCGAACAACCGGTACACCCGCCAGACGCGCGGCCTCTTCGTTCCCGCC                                                                                 <  SRR4302535.3773620/94‑1 (MQ=60)
                ATCGCTGATACCAGGCCTGAGAGAACAAAGATAATAATTTTGGTGCGAACAACCGGTACACCCGCCAGACGCGCGGCCTCTTCGTTCCCGCCAA                                                                               >  SRR4302535.2349010/1‑94 (MQ=60)
                 TCGCTGATACCAGGCCTGAGAGAACAAAGATAATAATTTTGGTGCGAACAACCGGTACACCCGCCAGACGCGCGGCCTCTTCGTTCCCGCCAAT                                                                              <  SRR4302535.3365650/94‑1 (MQ=60)
                  CGCTGATACCAGGCCTGAGAGAACAAAGATAATAATTTTGGTGCGAACAACCGGTACCCCCGCCAGACGCGCGGCCTCTTCGTTCCCGCCAATC                                                                             <  SRR4302535.1173480/94‑1 (MQ=60)
                   GCTGATACCAGGCCTGAGAGAACAAAGATAATAATTTTGGTGCGAACAACCGGTACACCCGCCAGACGCGCGGCCTCTTCGTTCCCGCCAATCG                                                                            >  SRR4302535.1453632/1‑94 (MQ=60)
                    CTGATACCAGGCCTGAGAGAACAAAGATAATAATTTTGGTGCGAACAACCGGTACACCCGCCAGACGCGCGGCCTCTTCGTTCCCGCCAATCGC                                                                           >  SRR4302535.3917116/1‑94 (MQ=60)
                    CTGATACCAGGCCTGAGAGAACAAAGATAATAATTTTGGTGCGAACAACCGGTACACCCGCCAGACGCGCGGCCTCTTCGTTCCCGCCAATCGC                                                                           >  SRR4302535.2011560/1‑94 (MQ=60)
                      GATACCAGGCCTGAGAGAACAAAGATAATAATTTTGGTGCGAACAACCGGTACACCCGCCAGACGCGCGGCCTCTTCGTTCCCGCCAATCGCCA                                                                         <  SRR4302535.2437069/94‑1 (MQ=60)
                      GATACCAGGCCTGAGAGAACAAAGATAATAATTTTGGTGCGAACAACCGGTACACCCGCCAGACGCGCGGCCTCTTCGTTCCCGCCAATCGCCA                                                                         <  SRR4302535.3221148/94‑1 (MQ=60)
                       GTACCCGGCCTGAGAGAACAAACATAATAATTTTGGTGCGAACAACCGGTACCCCCGCCAGACGCGCGGCCTCTTCGTTCCCGCCAATCGCCAG                                                                        <  SRR4302535.899699/94‑1 (MQ=60)
                       ATACCAGGCCTGAGAGAACAAAGATAATAATTTTGGTGCGAACAACCGGTACACCCGCCAGACGCGCGGCCTCTTCGTTCCCGCCAATCGCCAG                                                                        <  SRR4302535.2988051/94‑1 (MQ=60)
                       ATACCAGGCCTGAGAGAACAAAGATAATAATTTTGGTGCGAACAACCGGTACACCCGCCAGACGCGCGGCCTCTTCGTTCCCGCCAATCGCCAG                                                                        <  SRR4302535.3502386/94‑1 (MQ=60)
                        TACCAGGCCTGAGAGAACAAAGATAATAATTTTGGTGCGAACAACCGGTACACCCGCCAGACGCGCGGCCTCTTCGTTCCCGCCAATCGCCAGG                                                                       <  SRR4302535.4685850/94‑1 (MQ=60)
                         ACCAGGCCTGAGAGAACAAAGATAATAATTTTGGTGCGAACAACCGGTACACCCGCCAGACGCGCGGCCTCTTCGTTCCCGCCAATCGCCAGGG                                                                      <  SRR4302535.2638816/94‑1 (MQ=60)
                            AGGCCTGAGAGAACAAAGATAATAATTTTGGTGCGAACAACCGGTACACCCGCCAGACGCGCGGCCTCTTCGTTCCCGCCAATCGCCAGGGTGT                                                                   <  SRR4302535.1008365/94‑1 (MQ=60)
                             GGCCTGAGAGAACAAAGATAATAATTTTGGTGCGAACAACCGGTACACCCGCCAGACGCGCGGCCTCTTCGTTCCCGCCAATCGCCAGGGTGTT                                                                  >  SRR4302535.3066381/1‑94 (MQ=60)
                               CCTGAGAGAACAAAGATAATAATTTTGGTGCGAACAACCGGTACACCCGCCAGACGCGCGGCCTCTTCGTTCCCGCCAATCGCCAGGGTGTTAC                                                                >  SRR4302535.2512809/1‑94 (MQ=60)
                                  GAGAGAACAAAGATAATAATTTTGGTGCGAACAACCGGTACACCCGCCAGACGCGCGGCCTCTTCGTTCCCGCCAATCGCCAGGGTGTTACGAC                                                             <  SRR4302535.2772767/94‑1 (MQ=60)
                                   AGAGAACAAAGATAATAATTTGGGTGCGAACAACCGGTACCCCCGCCAGACGCGCGGCCTCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACC                                                            <  SRR4302535.147361/94‑1 (MQ=60)
                                    GAGAACAAAGATAATAATTTTGGTGCGAACAACCGGTACACCCGCCAGACGCGCGGCCTCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCA                                                           >  SRR4302535.3693254/1‑94 (MQ=60)
                                     AGAACAAAGATAATAATTTTGGTGCGAACAACCGGTACACCCGCCAGACGCGCGGCCTCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCAA                                                          >  SRR4302535.3983310/1‑94 (MQ=60)
                                       AACAAAGATAATAATTTTGGTGCGAACAACCGGTACACCCGCCAGACGCGCGGCCTCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCAAAG                                                        <  SRR4302535.3418991/94‑1 (MQ=60)
                                        ACAAAGATAATAATTTTGGTGCGAACAACCGGTACACCCGCCAGACGCGCGGCCTCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCAAAGG                                                       >  SRR4302535.4276292/1‑94 (MQ=60)
                                        ACAAAGATAATAATTTTGGTGCGAACAACCGGTACACCCGCCAGACGCGCGGCCTCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCAAAGG                                                       >  SRR4302535.4145281/1‑94 (MQ=60)
                                         CAAAGATAATAATTTTGGTGCGAACAACCGGTACACCCGCCAGACGCGCGGCCTCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCAAAGGT                                                      >  SRR4302535.1581353/1‑94 (MQ=60)
                                         CAAAGATAATAATTTTGGTGCGAACAACCGGTACACCCGCCAGACGCGCGGCCTCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCAAAGGT                                                      <  SRR4302535.2786400/94‑1 (MQ=60)
                                         CAAAGATAATAATTTTGGTGCGAACAACCGGTACACCCGCCAGACGCGCGGCCTCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCAAAGGG                                                      >  SRR4302535.1269594/1‑94 (MQ=60)
                                         AAAAGATAATAATTTGGGTGCGAACAACCGGTACACCCGCCAGACGCGCGGCCTCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCAAAGGT                                                      <  SRR4302535.1658212/94‑1 (MQ=60)
                                          AAAGATAATAATTTTGGTGCGAACAACCGGTACACCCGCCAGACGCGCGGCCTCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCAAAGGTG                                                     >  SRR4302535.3634096/1‑94 (MQ=60)
                                          AAAGATAATAATTTTGGTGCGAACAACCGGTACACCCGCCAGACGCGCGGCCTCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCAAAGGTG                                                     >  SRR4302535.3655026/1‑94 (MQ=60)
                                          AAAGATAATAATTTTGGTGCGAACAACCGGTACACCCGCCAGACGCGCGGCCTCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCAAAGGTG                                                     <  SRR4302535.2443993/94‑1 (MQ=60)
                                           AAGATAATAATTTTGGTGCGAACAACCGGTACACCCGCCAGACGCGCGGCCTCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCAAAGGTGG                                                    >  SRR4302535.3341464/1‑94 (MQ=60)
                                            AGATAATAATTTTGGTGCGAACAACCGTGACACCCGCCAGACGCGCGGCCTCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCAAAGGTGGT                                                   <  SRR4302535.1758294/94‑1 (MQ=60)
                                             GATAATAATTTTGGTGCGAACAACCGGTACACCCGCCAGACGCGCGGCCTCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCAAAGGTGGTT                                                  >  SRR4302535.2478711/1‑94 (MQ=60)
                                              ATAATAATTTTGGTGCGAACAACCGGTACACCCGCCAGACGCGCGGCCTCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCAAAGGTGGTTT                                                 <  SRR4302535.4171810/94‑1 (MQ=60)
                                              ATAATAATTTTGGTGCGAACAACCGGTACACCCGCCAGACGCGCGGCCTCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCAAAGGTGGTTT                                                 <  SRR4302535.339520/94‑1 (MQ=60)
                                                AATAATTTTGGTGCGAACAACCGGTACACCCGCCAGACGCGCGGCCTCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCAAAGGTGGTTTTA                                               >  SRR4302535.2688264/1‑94 (MQ=60)
                                                  TAATTTTGGTGCGAACAACCGGTACACCCGCCAGACGCGCGGCCTCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCAAAGGTGGTTTTATT                                             <  SRR4302535.435068/94‑1 (MQ=60)
                                                  TAATTTTGGTGCGAACAACCGGTACACCCGCCAGACGCGCGGCCTCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCAAAGGTGGTTTTATT                                             >  SRR4302535.3706093/1‑94 (MQ=60)
                                                   AATTTTGGTGCGAACAACCGGTACACCCGCCAGACGCGCGGCCTCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCAAAGGTGGTTTTATTC                                            <  SRR4302535.2820524/94‑1 (MQ=60)
                                                   AATTTTGGTGCGAACAACCGGTACACCCGCCAGACGCGCGGCCTCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCAAAGGTGGTTTTATTC                                            >  SRR4302535.563840/1‑94 (MQ=60)
                                                     TTTTGGTGCGAACAACCGGTACACCCGCCAGACGCGCGGCCTCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCAAAGGTGGTTTTATTCAG                                          <  SRR4302535.401354/94‑1 (MQ=60)
                                                      TTTGGTGCGAACAACCGGTACACCCGCCAGACGCGCGGCCTCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCAAAGGTGGTTTTATTCAGC                                         <  SRR4302535.4674178/94‑1 (MQ=60)
                                                       TTGGTGCGAACAACCGGTACACCCGCCAGACGCGCGGCCTCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCAAAGGTGGTTTTATTCAGCA                                        >  SRR4302535.3920665/1‑94 (MQ=60)
                                                        TGGTGCGAACAACCGGTACCCCCGCCAGACGCGCGGCCTCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCAAAGGTGGTTTTATTCAGCAG                                       <  SRR4302535.1162237/94‑1 (MQ=60)
                                                        TGGTGCGAACAACCGGTACACCCGCCAGACGCGCGGCCTCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCAAAGGTGGTTTTATTCAGCAG                                       <  SRR4302535.617175/94‑1 (MQ=60)
                                                        TGGTGCGAACAACCGGTACACCCGCCAGACGCGCGGCCTCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCAAAGGTGGTTTTATTCAGCAG                                       >  SRR4302535.1027532/1‑94 (MQ=60)
                                                             CGAACAACCGGTACGCCCGCCAGACGCGCGGCCTCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCAAAGGTGGTTTTATTCAGCAGCAAAC                                  <  SRR4302535.1465541/94‑1 (MQ=60)
                                                             CGAACAACCGGTACACCCGCCAGACGCGCGGCCTCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCAAAGGTGGTTTTATTCAGCAGCAAAC                                  >  SRR4302535.3699838/1‑94 (MQ=60)
                                                             CGAACAACCGGTACACCCGCCAGACGCGCGGCCTCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCAAAGGTGGTTTTATTCAGCAGCAAAC                                  >  SRR4302535.1613983/1‑94 (MQ=60)
                                                              GAACAACCGGTACACCCGCCAGACGCGCGGCCTCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCAAAGGTGGTTTTATTCAGCAGCAAACC                                 <  SRR4302535.1803040/94‑1 (MQ=60)
                                                               AACAACCGGTACACCCGCCAGACGCGCGGCCTCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCAAAGGTGGTTTTATTCAGCAGCAAACCA                                <  SRR4302535.4233259/94‑1 (MQ=60)
                                                                  acccggtcccCCCGCCAGACGCGCGGCCTCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCAAAGGTGGTTTTATTCAGCAGCAAACCAAAG                             <  SRR4302535.2825842/84‑1 (MQ=60)
                                                                  ACCCGGTACACCCGCCAGACGCGCGGCCTCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCAAAGGTGGTTTTATTCAGCAGCAAACCAAAG                             <  SRR4302535.3121191/94‑1 (MQ=60)
                                                                      GGTCCACCCGGCAGACGCGCGGCCTTTTGGTTCCCGCGAATCGCCAGGGTGTTACGACCAAAGGTGGTTTTATTCAGCAGCAAACCAAAGATAA                         <  SRR4302535.2483960/94‑1 (MQ=60)
                                                                      GGTACACCCGCCAGACGCGCGGCCTCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCAAAGGTGGTTTTATTCAGCAGCAAACCAAAGATAA                         <  SRR4302535.3192378/94‑1 (MQ=60)
                                                                      GGTACACCCGCCAGACGCGCGGCCTCTTCGTTCCCGCCAATCGACAGGGTGTTACGACCAAAGGTGGTTTTATTCAGCAGCAAACCAAAGATAA                         >  SRR4302535.2534624/1‑94 (MQ=60)
                                                                        TACACCCGCCAGACGCGCGGCCTCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCAAAGGTGGTTTTATTCAGCAGCAAACCAAAGATAATC                       <  SRR4302535.3984548/94‑1 (MQ=60)
                                                                         ACACCCGCCAGACGCGCGGCCTCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCAAAGGTGGTTTTATTCAGCAGCAAACCAAAGATAATCA                      >  SRR4302535.1122645/1‑94 (MQ=60)
                                                                          CACCCGCCAGACGCGCGGCCTCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCAAAGGTGGTTTTATTCAGCAGCAAACCAAAGATAATCAG                     <  SRR4302535.3931757/94‑1 (MQ=60)
                                                                          CACCCGCCAGACGCGCGGCCTCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCAAAGGTGGTTTTATTCAGCAGCAAACCAAAGATAATCAG                     <  SRR4302535.3091927/94‑1 (MQ=60)
                                                                            CCCGCCAGACGCGCGGCCTCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCAAAGGTGGTTTTATTCAGCAGCAAACCAAAGATAATCAGAC                   >  SRR4302535.1530434/1‑94 (MQ=60)
                                                                             CCGCCAGACGCGCGGCCTCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCAAAGGTGGTTTTATTCAGCAGCAAACCAAAGATAATCAGACA                  <  SRR4302535.1840740/94‑1 (MQ=60)
                                                                                CCAGACGCGCGGCCTCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCAAAGGTGGTTTTATTCAGCAGCAAACCAAAGATAATCAGACACGC               >  SRR4302535.4582300/1‑94 (MQ=60)
                                                                                 CAGACGCGCGGCCTCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCAAAGGTGGTTTTATTCAGCAGCAAACCAAAGATAATCAGACACGCG              <  SRR4302535.2142157/94‑1 (MQ=60)
                                                                                 CAGACGCGCGGCCTCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCAAAGGTGGTTTTATTCAGCAGCAAACCAAAGATAATCAGACACGCG              >  SRR4302535.4113275/1‑94 (MQ=60)
                                                                                  AGACGCGCGGCCTCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCAAAGGTGGTTTTATTCAGCAGCAAACCAAAGATAATCAGACACGCGA             >  SRR4302535.2245766/1‑94 (MQ=60)
                                                                                    ACGCGCGGCCTCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCAAAGGTGGTTTTATTCAGCAGCAAACCAAAGATAATCAGACACGCGACG           <  SRR4302535.1385640/94‑1 (MQ=60)
                                                                                         CGGCCTCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCAAAGGTGGTTTTATTCAGCAGCAAACCAAAGATAATCAGACACGCGACGGTGAG      <  SRR4302535.2008678/94‑1 (MQ=60)
                                                                                         CGGCCTCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCAAAGGTGGTTTTATTCAGCAGCAAACCAAAGATAATCAGACACGCGACGGTGAG      <  SRR4302535.2775806/94‑1 (MQ=60)
                                                                                         CGGCCTCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCAAAGGTGGTTTTATTCAGCAGCAAACCAAAGATAATCAGACACGCGACGGTGAG      >  SRR4302535.3730938/1‑94 (MQ=60)
                                                                                          ggcCTCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCAAAGGTGGTTTTATTCAGCAGCAAACCAAAGATAATCAGACACGCGACGGTGAGC     >  SRR4302535.2166957/4‑94 (MQ=60)
                                                                                          ggcCTCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCAAAGGTGGTTTTATTCAGCAGCAAACCAAAGATAATCAGACACGCGACGGCGAGC     >  SRR4302535.2349984/4‑94 (MQ=60)
                                                                                             CTCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCAAAGGTGGTTTTATTCAGCAGCAAACCAAAGATAATCAGACACGCGACGGTGAGCCAG  >  SRR4302535.3609861/1‑94 (MQ=60)
                                                                                             CTCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCAAAGGTGGTTTTATTCAGCAGCAAACCAAAGATAATCAGACACGCGACGGTGAGCCAG  >  SRR4302535.3496303/1‑94 (MQ=60)
                                                                                             |                                                                                             
CAGAATAATTCCGGCTATCGCTGATACCAGGCCTGAGAGAACAAAGATAATAATTTTGGTGCGAACAACCGGTACACCCGCCAGACGCGCGG‑CTCTTCGTTCCCGCCAATCGCCAGGGTGTTACGACCAAAGGTGGTTTTATTCAGCAGCAAACCAAAGATAATCAGACACGCGACGGTGAGCCAG  >  NC_000913/1980848‑1981033

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑