Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I2 R1	1873	183.1	9376148	98.2%	9207377	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,107,690	C→T	W157* (TGG→TGA)	rfbC ←	dTDP‑6‑deoxy‑D‑glucose‑3,5 epimerase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,107,690	0	C	T	99.3%	484.3 / ‑5.4	142	W157* (TGG→TGA)	rfbC	dTDP‑6‑deoxy‑D‑glucose‑3,5 epimerase
Reads supporting (aligned to +/- strand): ref base C (0/1); new base T (67/74); total (67/75)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.04e-01

Only 100 of 144 total aligned reads displayed.

ATTCATGCAATTAATTTTAATCTGATAAGCTCATCTAACGTAAAGAGCCTTTCATCTTTTGGCGAAAGGATTAACCCTGATGTTTGGGGCCAATCAATTGCAATGCGTTCATCATTCCAACATATTCCACAATCGCTTTCAGGATGATAATAGTTTGTAGTTTTATATTGAAATTC  >  NC_000913/2107601‑2107776

aTTCATGCAATTAATTTTAATCTGATAAGCTCATCTAACGTAAAGAGCCTTTCATCTTTTGGCGAAAGGATTAACCCTGATGTTTGGGGTcaa                                                                                     <  1:4380917/93‑1 (MQ=255)

aTTCATGCAATTAATTTTAATCTGATAAGCTCATCTAACGTAAAGAGCCTTTCATCTTTTGGCGAAAGGATTAACCCTGATGTTTGGGGTcaa                                                                                     >  2:1326437/1‑93 (MQ=255)

 ttCATGCAATTAATTTTAATCTGATAAGCTCATCTAACGTAAAGAGCCTTTCATCTTTTGGCGAAAGGATTAACCCTGATGTTTGGGGTcaat                                                                                    >  1:808926/1‑93 (MQ=255)

 ttCATGCAATTAATTTTAATCTGATAAGCTCATCTAACGTAAAGAGCCTTTCATCTTTTGGCGAAAGGATTAACCCTGATGTTTGGGGTcaat                                                                                    >  1:2666152/1‑93 (MQ=255)

 ttCATGCAATTAATTTTAATCTGATAAGCTCATCTAACGTAAAGAGCCTTTCATCTTTTGGCGAAAGGATTAACCCTGATGTTTGGGGTcaat                                                                                    >  1:2401742/1‑93 (MQ=255)

 ttCATGCAATTAATTTTAATCTGATAAGCTCATCTAACGTAAAGAGCCTTTCATCTTTTGGCGAAAGGATTAACCCTGATGTTTGGGGTcaat                                                                                    >  2:559938/1‑93 (MQ=255)

  tCATGCAATTAATTTTAATCTGATAAGCTCATCTAACGTAAAGAGCCTTTCATCTTTTGGCGAAAGGATTAACCCTGATGTTTGGGGTcaatc                                                                                   <  1:2120207/93‑1 (MQ=255)

      gCAATTAATTTTAATCTGATAAGCTCATCTAACGTAAAGAGCCTTTCATCTTTTGGCGAAAGGATTAACCCTGATGTTTGGGGTCAATCAAtt                                                                               <  1:479905/93‑1 (MQ=255)

      gCAATTAATTTTAATCTGATAAGCTCATCTAACGTAAAGAGCCTTTCATCTTTTGGCGAAAGGATTAACCCTGATGTTTGGGGTCAATCAAtt                                                                               >  2:1780060/1‑93 (MQ=255)

        aattaattTTAATCTGATAAGCTCATCTAACGTAAAGAGCCTTTCATCTTTTGGCGAAAGGATTAACCCTGATGTTTGGGGTCAATCAATTga                                                                             >  2:2648553/1‑92 (MQ=255)

        aattaattTTAATCTGATAAGCTCATCTAACGTAAAGAGCCTTTCATCTTTTGGCGAAAGGATTAACCCTGATGTTTGGGGTCAATCAATTGc                                                                             >  1:3243585/1‑93 (MQ=255)

        aattaattTTAATCTGATAAGCTCATCTAACGTAAAGAGCCTTTCATCTTTTGGCGAAAGGATTAACCCTGATGTTTGGGGTCAATCAATTGc                                                                             >  1:846706/1‑93 (MQ=255)

         attaattTTAATCTGATAAGCTCATCTAACGTAAAGAGCCTTTCATCTTTTGGCGAAAGGATTAACCCTGATGTTTGGGGTCAATCAATTGCa                                                                            <  1:4530211/93‑1 (MQ=255)

         attaattTTAATCTGATAAGCTCATCTAACGTAAAGAGCCTTTCATCTTTTGGCGAAAGGATTAACCCTGATGTTTGGGGTCAATCAATTGCa                                                                            >  1:323072/1‑93 (MQ=255)

          ttaattTTAATCTGATAAGCTCATCTAACTTAAAGAGCCTTTCATCTTTTGGCGAAAGGATTAACCCTGATGTTTGGGGTCAATCAATTGCaa                                                                           <  2:4398746/93‑1 (MQ=255)

          ttaattTTAATCTGATAAGCTCATCTAACGTAAAGAGCCTTTCATCTTTTGGCGAAAGGATTAACCCTGATGTTTGGGGTCAATCAATTGCaa                                                                           <  1:356025/93‑1 (MQ=255)

           taattTTAATCTGATAAGCTCATCTAACGTAAAGAGCCTTTCATCTTTTGGCGAAAGGATTAACCCTGATGTTTGGGGTCAATCAATTGCAAt                                                                          <  2:3196313/93‑1 (MQ=255)

              ttttAATCTGATAAGCTCATCTAACGTAAAGAGCCTTTCATCTTTTGGCGAAAGGATTAACCCTGATGTTTGGGGTCAATCAATTGCAATGCg                                                                       >  2:692914/1‑93 (MQ=255)

                 tAATCTGATAAGCTCATCTAACGTAAAGAGCCTTTCATCTTTTGGCGAAAGGATTAACCCTGATGTTTGGGGTCAATCAATTGCAATGCGTtc                                                                    <  2:1959467/93‑1 (MQ=255)

                 tAATCTGATAAGCTCATCTAACGTAAAGAGCCTTTCATCTTTTGGCGAAAGGATTAACCCTGATGTTTGGGGTCAATCAATTGCAATGCGTtc                                                                    >  2:2023961/1‑93 (MQ=255)

                     cTGATAAGCTCATCTAACGTAAAGAGCCTTTCATCTTTTGGCGAAAGGATTAACCCTGATGTTTGGGGTCAATCAATTGCAATGCGTtcatca                                                                <  1:2981059/93‑1 (MQ=255)

                     cTGATAAGCTCATCTAACGTAAAGAGCCTTTCATCTTTTGGCGAAAGGATTAACCCTGATGTTTGGGGTCAATCAATTGCAATGCGTtcatca                                                                <  1:187845/93‑1 (MQ=255)

                      tGATAAGCTCATCTAACGTAAAGAGCCTTTCATCTTTTGGCGAAAGGATTAACCCTGATGTTTGGGGTCAATCAATTGCAATGCGTTcatcat                                                               >  2:666450/1‑93 (MQ=255)

                      tGATAAGCTCATCTAACGTAAAGAGCCTTTCATCTTTTGGCGAAAGGATTAACCCTGATGTTTGGGGTCAATCAATTGCAATGCGTTcatcat                                                               >  1:1337781/1‑93 (MQ=255)

                      tGATAAGCTCATCTAACGTAAAGAGCCTTTCATCTTTTGGCGAAAGGATTAACCCTGATGTTTGGGGTCAATCAATTGCAATGCGTTcatcat                                                               <  2:542193/93‑1 (MQ=255)

                      tGATAAGCTCATCTAACGTAAAGAGCCTTTCATCTTTTGGCGAAAGGATTAACCCTGATGTTTGGGGTCAATCAATTGCAATGCGTTcatcat                                                               >  2:458/1‑93 (MQ=255)

                      tGATAAGCTCATCTAACGTAAAGAGCCTTTCATCTTTTGGCGAAAGGATTAACCCTGATGTTTGGGGTCAATCAATTGCAATGCGTTcatcat                                                               <  2:1425431/93‑1 (MQ=255)

                         tAAGCTCATCTAACGTAAAGAGCCTTTCATCTTTTGGCGAAAGGATTAACCCTGATGTTTGGGGTCAATCAATTGCAATGCGTTCATCATTcc                                                            >  2:321297/1‑93 (MQ=255)

                         tAAGCTCATCTAACGTAAAGAGCCTTTCATCTTTTGGCGAAAGGATTAACCCTGATGTTTGGGGTCAATCAATTGCAATGCGTTCATCATTcc                                                            >  2:393156/1‑93 (MQ=255)

                         tAAGCTCATCTAACGTAAAGAGCCTTTCATCTTTTGGCGAAAGGATTAACCCTGATGTTTGGGGTCAATCAATTGCAATGCGTTCATCATTcc                                                            >  2:1266504/1‑93 (MQ=255)

                          aaGCTCATCTAACGTAAAGAGCCTTTCATCTTTTGGCGAAAGGATTAACCCTGATGTTTGGGGTCAATCAATTGCAATGCGTTCATCATTCCa                                                           >  2:4631608/1‑93 (MQ=255)

                           aGCTCATCTAACGTAAAGAGCCTTTCATCTTTTGGCGAAAGGATTAACCCTGATGTTTGGGGTCAATCAATTGCAATGCGTTCATCATTCCaa                                                          >  2:507995/1‑93 (MQ=255)

                             cTCATCTAACGTAAAGAGCCTTTCATCTTTTGGCGAAAGGATTAACCCTGATGGTTGGGGTCAATCAATTGCAATGCGTTCATCATTCCAACa                                                        >  2:3296045/1‑93 (MQ=255)

                               cATCTAACGTAAAGAGCCTTTCATCTTTTGGCGAAAGGATTAACCCTGATGTTTGGGGTCAATCAATTGCAATGCGTTCATCATTCCAACata                                                      <  2:4618024/93‑1 (MQ=255)

                               cATCTAACGTAAAGAGCCTTTCATCTTTTGGCGAAAGGATTAACCCTGATGTTTGGGGTCAATCAATTGCAATGCGTTCATCATTCCAACata                                                      <  1:4262144/93‑1 (MQ=255)

                                aTCTAACGTAAAGAGCCTTTCATCTTTTGGCGAAAGGATTAACCCTGATGTTTGGGGTCAATCAATTGCAATGCGTTCATCATTCCAACatat                                                     <  1:3897270/93‑1 (MQ=255)

                                 tCTAACGTAAAGAGCCTTTCATCTTTTGGCGAAAGGATTAACCCTGATGTTTGGGGTCAATCAATTGCAATGCGTTCATCATTCCAACATAtt                                                    >  2:1631930/1‑93 (MQ=255)

                                 tCTAACGTAAAGAGCCTTTCATCTTTTGGCGAAAGGATTAACCCTGATGTTTGGGGTCAATCAATTGCAATGCGTTCATCATTCCAACATAtt                                                    <  1:4084616/93‑1 (MQ=255)

                                 tCTAACGTAAAGAGCCTTTCATCTTTTGGCGAAAGGATTAACCCTGATGTTTGGGGTCAATCAATTGCAATGCGTTCATCATTCCAACATAtt                                                    <  2:2059938/93‑1 (MQ=255)

                                 tCTAACGTAAAGAGCCTTTCATCTTTTGGCGAAAGGATTAACCCTGATGTTTGGGGTCAATCAATTGCAATGCGTTCATCATTCCAACATAtt                                                    >  1:397239/1‑93 (MQ=255)

                                  cTAACGTAAAGAGCCTTTCATCTTTTGGCGAAAGGATTAACCCTGATGTTTGGGGTCAATCAATTGCAATGCGTTCATCATTCCAACATATTc                                                   <  1:3678418/93‑1 (MQ=255)

                                     aCGTAAAGAGCCTTTCATCTTTTGGCGAAAGGATTAACCCTGATGTTTGGGGTCAATCAATTGCAATGCGTTCATCATTCCAACATATTCcac                                                <  1:364827/93‑1 (MQ=255)

                                     aCGTAAAGAGCCTTTCATCTTTTGGCGAAAGGATTAACCCTGATGTTTGGGGTCAATCAATTGCAATGCGTTCATCATTCCAACATATTCcac                                                >  1:952719/1‑93 (MQ=255)

                                       gTAAAGAGCCTTTCATCTTTTGGCGAAAGGATTAACCCTGATGTTTGGGGTCAATCAATTGCAATGCGTTCATCATTCCAACATATTCCACaa                                              <  2:1494335/93‑1 (MQ=255)

                                       gTAAAGAGCCTTTCATCTTTTGGCGAAAGGATTAACCCTGATGTTTGGGGTCAATCAATTGCAATGCGTTCATCATTCCAACATATTCCACaa                                              <  1:3081251/93‑1 (MQ=255)

                                        tAAAGAGCCTTTCATCTTTTGGCGAAAGGATTAACCCTGATGTTTGGGGTCAATCAATTGCAATGCGTTCATCATTCCAACATATTCCACAAt                                             >  1:3725095/1‑93 (MQ=255)

                                          aaGAGCCTTTCATCTTTTGGCGAAAGGATTAACCCTGATGTTTGGGGTCAATCAATTGCAATGCGTTCATCATTCCAACATATTCCACAATCg                                           >  2:2997717/1‑93 (MQ=255)

                                           agagCCTTTCATCTTTTGGCGAAAGGATTAACCCTGATGTTTGGGGTCAATCAATTGCAATGCGTTCATCATTCCAACATATTCCACAATCGc                                          >  2:4166146/1‑93 (MQ=255)

                                            gagCCTTTCATCTTTTGGCGAAAGGATTAACCCTGATGTTTGGGGTCAATCAATTGCAATGCGTTCATCATTCCAACATATTCCACAATCGCt                                         <  2:4168306/93‑1 (MQ=255)

                                               cttttCATCTTTTGGCGAAAGGATTAACCCTGATTTTTGGGGTCAATCAATTGCAATGCGTTCATCATTCCAACATATTCCACAATCGCTTTc                                      <  2:827421/91‑1 (MQ=255)

                                               ccTTTCATCTTTTGGCGGAAGGATTAACCCTGATGGTTGGGGTCAATCAATTGCAATGCGTTCATCATTCCAACATATTCCACAATCGCTTTc                                      >  1:2400500/1‑93 (MQ=255)

                                                cTTTCATCTTTTGGCGAAAGGATTAACCCTGATGTTTGGGGTCAATCAATTGCAATGCGTTCATCATTCCAACATATTCCACAATCGCTTTCa                                     >  1:4100319/1‑93 (MQ=255)

                                                 tttCATCTTTTGGCGAAAGGATTAACCCTGATGTTTGGGGTCAATCAATTGCAATGCGTTCATCATTCCAACATATTCCACAATCGCTTTCAg                                    >  2:3487741/1‑93 (MQ=255)

                                                 tttCATCTTTTGGCGAAAGGATTAACCCTGATGTTTGGGGTCAATCAATTGCAATGCGTTCATCATTCCAACATATTCCACAATCGCTTTCAg                                    <  2:1226192/93‑1 (MQ=255)

                                                  ttCATCTTTTGGCGAAAGGATTAACCCTGATGTTTGGGGTTAATCAATTGCAATGCGTTCATCATTCCAACATATTCCACAATCGCTTTCAgg                                   >  1:4064329/1‑93 (MQ=255)

                                                  ttCATCTTTTGGCGAAAGGATTAACCCTGATGTTTGGGGTCAATCAATTGCAATGCGTTCATCATTCCAACATATTCCACAATCGCTTTCAgg                                   <  2:4423515/93‑1 (MQ=255)

                                                   tCATCTTTTGGCGAAAGGATTAACCCTGATGTTTGGGGTCAATCAATTGCAATGCGTTCATCATTCCAACATATTCCACAATCGCTTTCAGga                                  >  2:222031/1‑93 (MQ=255)

                                                   tCATCTTTTGGCGAAAGGATTAACCCTGATGTTTGGGGTCAATCAATTGCAATGCGTTCATCATTCCAACATATTCCACAATCGCTTTCAGga                                  <  2:3949158/93‑1 (MQ=255)

                                                   tCATCTTTTGGCGAAAGGATTAACCCTGATGTTTGGGGTCAATCAATTGCAATGCGTTCATCATTCCAACATATTCCACAATCGCTTTCAGga                                  <  1:1575673/93‑1 (MQ=255)

                                                   tCATCTTTTGGCGAAAGGATTAACCCTGATGTTTGGAGTCAATCAATTGCAATGCGTTCATCATTCCAACATATTCCACAATCGCTTTCAGga                                  <  2:2769478/93‑1 (MQ=255)

                                                   gcATCTTTTGGCGAAAGGATTAACCCTGATGTTTGGGGTCAATCAATTGCAATGCGTTCATCATTCCAACATATTCCACAATCGCTTTCAGga                                  >  2:4503956/2‑93 (MQ=255)

                                                    cATCTTTTGGCGAAAGGATTAACCCTGATGTTTGGGGTCAATCAATTGCAATGCGTTCATCATTCCAACATATTCCACAATCGCTTTCAGgat                                 <  2:818536/93‑1 (MQ=255)

                                                     aTCTTTTGGCGAAAGGATTAACCCTGATGTTTGGGGTCAATCAATTGCAATGCGTTCATCATTCCAACATATTCCACAATCGCTTTCAGgatg                                >  1:5122/1‑93 (MQ=255)

                                                     aTCTTTTGGCGAAAGGATTAACCCTGATGTTTGGGGTCAATCAATTGCAATGCGTTCATCATTCCAACATATTCCACAATCGCTTTCAGgatg                                <  1:4376228/93‑1 (MQ=255)

                                                      tCTTTTGGCGAAAGGATTAACCCTGATGTTTGGGGTCAATCAATTGCAATGCGTTCATCATTCCAACATATTCCACAATCGCTTTCAGgatga                               >  1:4574469/1‑93 (MQ=255)

                                                       cTTTTGGCGAAAGGATTAACCCTGATGTTTGGGGTCAATCAATTGCAATGCGTTCATCATTCCAACATATTCCACAATCGCTTTCAGgatgat                              <  2:3818953/93‑1 (MQ=255)

                                                       cTTTTGGCGAAAGGATTAACCCTGATGTTTGGGGTCAATCAATTGCAATGCGTTCATCATTCCAACATATTCCACAATCGCTTTCAGgatgat                              <  1:2128332/93‑1 (MQ=255)

                                                        ttttGGCGAAAGGATTAACCCTGTTGCTTGGGGTCAATTAATTGCAATGCGTTCATCATTCCAACATATTCCACAATCGCTTTGAGGATGata                             >  2:2042784/1‑93 (MQ=255)

                                                        ttttGGCGAAAGGATTAACCCTGATGTTTGGGGTCAATCAATTGCAATGCGTTCATCATTCCAACATATTCCACAATCGCTTTCAGGATGata                             >  1:538588/1‑93 (MQ=255)

                                                         tttGGCGAAAGGATTAACCCTGATGTTTGGGGTCAATCAATTGCAATGCGTTCATCATTCCAACATATTCCACAATCGCTTTCAGGATGataa                            <  1:4480644/93‑1 (MQ=255)

                                                         tttGGCGAAAGGATTAACCCTGATGTTTGGGGTCAATCAATTGCAATGCGTTCATCATTCCAACATATTCCACAATCGCTTTCAGGATGataa                            >  2:2885890/1‑93 (MQ=255)

                                                            ggCGAAAGGATTAACCCTGATGTTTGGGGTCAATCAATTGCAATGCGTTCATCATTCCAACATATTCCACAATCGCTTTCAGGATGATAATAg                         <  1:4168511/93‑1 (MQ=255)

                                                            ggCGAAAGGATTAACCCTGATGTTTGGAGTCAATCAATTGCAATGCGTTCATCATTCCAACATATTCCACAATCGCTTTCAGGATGATAATAg                         <  1:497447/93‑1 (MQ=255)

                                                             gCGAAAGGATTAACCCTGATGTTTGGGGTCAATCAATTGCAATGCGTTCATCATTCCAACATATTCCACAATCGCTTTCAGGATGATAATAGt                        <  1:3849865/93‑1 (MQ=255)

                                                              cGAAAGGATTAACCCTGATGTTTGGGGTCAATCAATTGCAATGCGTTCATCATTCCAACATATTCCACAATCGCTTTCAGGATGATAATAGtt                       <  1:3513251/93‑1 (MQ=255)

                                                               gAAAGGATTAACCCTGATGTTTGGGGTCAATCAATTGCAATGCGTTCATCATTCCAACATATTCCACAATCGCTTTCAGGATGATAATAGttt                      <  2:4373394/93‑1 (MQ=255)

                                                               gAAAGGATTAACCCTGATGTTTGGGGTCAATCAATTGCAATGCGTTCATCATTCCAACATATTCCACAATCGCTTTCAGGATGATAATAGttt                      <  1:1310210/93‑1 (MQ=255)

                                                                aaaGGATTAACCCTGATGTTTGGGGTCAATCAATTGCAATGCGTTCATCATTCCAACATATTCCACAATCGCTTTCAGGATGATAATAGTTTg                     >  1:2551915/1‑93 (MQ=255)

                                                                aaaGGATTAACCCTGATGTTTGGGGTCAATCAATTGCAATGCGTTCATCATTCCAACATATTCCACAATCGCTTTCAGGATGATAATAGTTTg                     >  2:4209999/1‑93 (MQ=255)

                                                                aaaGGATTAACCCTGATGTTTGGGGTCAATCAATTGCAATGCGTTCATCATTCCAACATATTCCACAATCGCTTTCAGGATGATAATAGTTTg                     >  1:2551914/1‑93 (MQ=255)

                                                                 aaGGATTAACCCTGATGTTTGGGGTCAATCAATTGCAATGCGTTCATCATTCCAACATATTCCACAATCGCTTTCAGGATGATAATAGTTTGt                    >  1:4316831/1‑93 (MQ=255)

                                                                  aGGATTAACCCTGATGTTTGGGGTCAATCAATTGCAATGCGTTCATCATTCCAACATATTCCACAATCGCTTTCAGGATGATAATAGTTTGTa                   <  1:663079/93‑1 (MQ=255)

                                                                  aGGATTAACCCTGATGTTTGGGGTCAATCAATTGCAATGCGTTCATCATTCCAACATATTCCACAATCGCTTTCAGGATGATAATAGTTTGTa                   <  2:2510601/93‑1 (MQ=255)

                                                                    gATTAACCCTGATGTTTGGGGCCAATCAATTGCAATGCGTTCATCATTCCAACATATTCCACAATCGCTTTCAGGATGATAATAGTTTGTAGt                 <  2:4329591/93‑1 (MQ=255)

                                                                      ttAACCCTGATGTTTGGGGTCAATCAATTGCAATGCGTTCATCATTCCAACATATTCCACAATCGCTTTCAGGATGATAATAGTTTGTAGttt               <  2:2669083/93‑1 (MQ=255)

                                                                      ttAACCCTGATGTTTGGGGTCAATCAATTGCAATGCGTTCATCATTCCAACATATTCCACAATCGCTTTCAGGATGATAATAGTTTGTAGttt               >  1:2350499/1‑93 (MQ=255)

                                                                         aCCCTGATGTTTGGGGTCAATCAATTGCAATGCGTTCATCATTCCAACATATTCCACAATCGCTTTCAGGATGATAATAGTTTGTAGTTTtat            >  1:1612031/1‑93 (MQ=255)

                                                                         aCCCTGATGTTTGGGGTCAATCAATTGCAATGCGTTCATCATTCCAACATATTCCACAATCGCTTTCAGGATGATAATAGTTTGTAGTTTtat            >  1:650686/1‑93 (MQ=255)

                                                                          cccTGATGTTTGGGGTCAATCAATTGCAATGCGTTCATCATTCCAACATATTCCACAATCGCTTTCAGGATGATAATAGTTTGTAGTTTtata           >  2:35883/1‑93 (MQ=255)

                                                                           ccTGATGTTTGGGGTCAATCAATTGCAATGCGTTCATCATTCCAACATATTCCACAATCGCTTTCAGGATGATAATAGTTTGTAGTTTTatat          <  1:1070060/93‑1 (MQ=255)

                                                                           ccTGATGTTTGCGGTCAATCAATTGCAATGCGTTCATCATTCCAACATATTCCACAATCGCTTTCAGGATGATAATAGTTTGTAGTTTTatat          <  1:1063546/93‑1 (MQ=255)

                                                                            cTGATGTTTGGGGTCAATCAATTGCAATGCGTTCATCATTCCAACATATTCCACAATCGCTTTCAGGATGATAATAGTTTGTAGTTTTATAtt         >  2:3777269/1‑93 (MQ=255)

                                                                            cTGATGTTTGGGGTCAATCAATTGCAATGCGTTCATCATTCCAACATATTCCACAATCGCTTTCAGGATGATAATAGTTTGTAGTTTTATAtt         <  1:3323254/93‑1 (MQ=255)

                                                                            cTGATGTTTGGGGTCAATCAATTGCAATGCGTTCATCATTCCAACATATTCCACAATCGCTTTCAGGATGATAATAGTTTGTAGTTTTATAtt         >  2:3041171/1‑93 (MQ=255)

                                                                             tGATGTTTGGGGTCAATCAATTGCAATGCGTTCATCATTCCAACATATTCCACAATCGCTTTCAGGATGATAATAGTTTGTAGTTTTATATTg        >  1:2391732/1‑93 (MQ=255)

                                                                              gATGTTTGGGGTCAATCAATTGCAATGCGTTCATCATTCCAACATATTCCACAATCGCTTTCAGGATGATAATAGTTTGTAGTTTTATATTGa       >  1:1060853/1‑93 (MQ=255)

                                                                                tGTTTGGGGTCAATCAATTGCAATGCGTTCATCATTCCAACATATTCCACAATCGCTTTCAGGATGATAATAGTTTGTAGTTTTATATTGaaa     >  1:1862399/1‑93 (MQ=255)

                                                                                tGTTTGGGGTCAATCAATTGCAATGCGTTCATCATTCCAACATATTCCACAATCGCTTTCAGGATGATAATAGTTTGTAGTTTTATATTGaaa     >  2:2151947/1‑93 (MQ=255)

                                                                                 gTTTGGGGTCAATCAATTGCAATGCGTTCATCATTCCAACATATTCCACAATCGCTTTCAGGATGATAATAGTTTGTAGTTTTATATTGAAAt    >  1:2880382/1‑93 (MQ=255)

                                                                                  tttGGGGTCAATCAATTGCAATGCGTTCATCATTCCAACATATTCCACAATCGCTTTCAGGATGATAATAGTTTGTAGTTTTATATTGAAAtt   <  1:1738618/93‑1 (MQ=255)

ATTCATGCAATTAATTTTAATCTGATAAGCTCATCTAACGTAAAGAGCCTTTCATCTTTTGGCGAAAGGATTAACCCTGATGTTTGGGGCCAATCAATTGCAATGCGTTCATCATTCCAACATATTCCACAATCGCTTTCAGGATGATAATAGTTTGTAGTTTTATATTGAAATTC  >  NC_000913/2107601‑2107776

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 24 ≤` `ATCG/ATCG` `< 31 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

TCGTATTCATGCAATTAATTTTAATCTGATAAGCTCATCTAACGTAAAGAGCCTTTCATCTTTTGGCGAAAGGATTAACCCTGATGTTTGGGGCCAATCAATTGCAATGCGTTCATCATTCCAACATATTCCACAATCGCTTTCAGGATGATAATAGTTTGTAGTTTTATATTGAAATTCAGCGAT  >  NC_000913/2107597‑2107782

TCGTATTCATGCAATTAATTTTAATCTGATAAGCTCATCTAACGTAAAGAGCCTTTCATCTTTTGGCGAAAGGATTAACCCTGATGTTTGGGGT                                                                                              >  SRR4302535.2597608/1‑94 (MQ=60)

    ATTCATGCAATTAATTTTAATCTGATAAGCTCATCTAACGTAAAGAGCCTTTCATCTTTTGGCGAAAGGATTAACCCTGATGTTTGGGGTCAAT                                                                                          >  SRR4302535.2685239/1‑94 (MQ=60)

    ATTCATGCAATTAATTTTAATCTGATAAGCTCATCTAACGTAAAGAGCCTTTCATCTTTTGGCGAAAGGATTAACCCTGATGTTTGGGGTCAAT                                                                                          >  SRR4302535.2419017/1‑94 (MQ=60)

    ATTCATGCAATTAATTTTAATCTGATAAGCTCATCTAACGTAAAGAGCCTTTCATCTTTTGGCGAAAGGATTAACCCTGATGTTTGGGGTCAAT                                                                                          >  SRR4302535.814587/1‑94 (MQ=60)

    ATTCATGCAATTAATTTTAATCTGATAAGCTCATCTAACGTAAAGAGCCTTTCATCTTTTGGCGAAAGGATTAACCCTGATGTTTGGGGTCAAT                                                                                          <  SRR4302535.4410890/94‑1 (MQ=60)

      TCATGCAATTAATTTTAATCTGATAAGCTCATCTAACGTAAAGAGCCTTTCATCTTTTGGCGAAAGGATTAACCCTGATGTTTGGGGTCAATCA                                                                                        <  SRR4302535.2135498/94‑1 (MQ=60)

          GCAATTAATTTTAATCTGATAAGCTCATCTAACGTAAAGAGCCTTTCATCTTTTGGCGAAAGGATTAACCCTGATGTTTGGGGTCAATCAATTG                                                                                    <  SRR4302535.483273/94‑1 (MQ=60)

           CAATTAATTTTAATCTGATAAGCTCATCTAACGTAAAGAGCCTTTCATCTTTTGGCGAAAGGATTAACCCTGATGTTTGGGGTCAATCAATTGC                                                                                   >  SRR4302535.3266116/1‑94 (MQ=60)

           CAATTAATTTTAATCTGATAAGCTCATCTAACGTAAAGAGCCTTTCATCTTTTGGCGAAAGGATTAACCCTGATGTTTGGGGTCAATCAATTGC                                                                                   >  SRR4302535.852653/1‑94 (MQ=60)

            AATTAATTTTAATCTGATAAGCTCATCTAACGTAAAGAGCCTTTCATCTTTTGGCGAAAGGATTAACCCTGATGTTTGGGGTCAATCAATTGCA                                                                                  >  SRR4302535.325306/1‑94 (MQ=60)

             ATTAATTTTAATCTGATAAGCTCATCTAACGTAAAGAGCCTTTCATCTTTTGGCGAAAGGATTAACCCTGATGTTTGGGGTCAATCAATTGCAA                                                                                 <  SRR4302535.4561224/94‑1 (MQ=60)

              TTAATTTTAATCTGATAAGCTCATCTAACGTAAAGAGCCTTTCATCTTTTGGCGAAAGGATTAACCCTGATGTTTGGGGTCAATCAATTGCAAT                                                                                <  SRR4302535.358483/94‑1 (MQ=60)

                      AATCTGATAAGCTCATCTAACGTAAAGAGCCTTTCATCTTTTGGCGAAAGGATTAACCCTGATGTTTGGGGTCAATCAATTGCAATGNGTTNAT                                                                        >  SRR4302535.1489507/1‑94 (MQ=60)

                         CTGATAAGCTCATCTAACGTAAAGAGCCTTTCATCTTTTGGCGAAAGGATTAACCCTGATGTTTGGGGTCAATCAATTGCAATGCGTTCATCAT                                                                     <  SRR4302535.567568/94‑1 (MQ=60)

                         CTGATAAGCTCATCTAACGTAAAGAGCCTTTCATCTTTTGGCGAAAGGATTAACCCTGATGTTTGGGGTCAATCAATTGCAATGCGTTCATCAT                                                                     <  SRR4302535.189145/94‑1 (MQ=60)

                         CTGATAAGCTCATCTAACGTAAAGAGCCTTTCATCTTTTGGCGAAAGGATTAACCCTGATGTTTGGGGTCAATCAATTGCAATGCGTTCATCAT                                                                     >  SRR4302535.1347325/1‑94 (MQ=60)

                         CTGATAAGCTCATCTAACGTAAAGAGCCTTTCATCTTTTGGCGAAAGGATTAACCCTGATGTTTGGGGTCAATCAATTGCAATGCGTTCATCAT                                                                     <  SRR4302535.3001969/94‑1 (MQ=60)

                          TGATAAGCTCATCTAACGTAAAGAGCCTTTCATCTTTTGGCGAAAGGATTAACCCTGATGTTTGGGGTCAATCAATTGCAATGCGTTCATCATT                                                                    >  SRR4302535.2425325/1‑94 (MQ=60)

                                 CTCATCTAACGTAAAGAGCCTTTCATCTTTTGGCGAAAGGATTAACCCTGATGTTTGGGGTCAATCAATTGCAATGCGTTCATCATTCCAACAT                                                             >  SRR4302535.4124285/1‑94 (MQ=60)

                                 CTCATCTAACGTAAAGAGCCTTTCATCTTTTGGCGAAAGGATTAACCCTGATGTTTGGGGTCAATCAATTGCAATGCGTTCATCATTCCAACAT                                                             <  SRR4302535.4659457/94‑1 (MQ=60)

                                   CATCTAACGTAAAGAGCCTTTCATCTTTTGGCGAAAGGATTAACCCTGATGTTTGGGGTCAATCAATTGCAATGCGTTCATCATTCCAACATAT                                                           <  SRR4302535.4291334/94‑1 (MQ=60)

                                    ATCTAACGTAAAGAGCCTTTCATCTTTTGGCGAAAGGATTAACCCTGATGTTTGGGGTCAATCAATTGCAATGCGTTCATCATTCCAACATATT                                                          >  SRR4302535.400023/1‑94 (MQ=60)

                                    ATCTAACGTAAAGAGCCTTTCATCTTTTGGCGAAAGGATTAACCCTGATGTTTGGGGTCAATCAATTGCAATGCGTTCATCATTCCAACATATT                                                          <  SRR4302535.3923976/94‑1 (MQ=60)

                                     TCTAACGTAAAGAGCCTTTCATCTTTTGGCGAAAGGATTAACCCTGATGTTTGGGGTCAATCAATTGCAATGCGTTCATCATTCCAACATATTC                                                         <  SRR4302535.4112547/94‑1 (MQ=60)

                                      CTAACGTAAAGAGCCTTTCATCTTTTGGCGAAAGGATTAACCCTGATGTTTGGGGTCAATCAATTGCAATGCGTTCATCATTCCAACATATTCC                                                        <  SRR4302535.3703878/94‑1 (MQ=60)

                                        AACGTAAAGAGCCTTTCATCTTTTGGCGAAAGGATTAACCCTGATGTTTGGGGTCAATCAATTGCAATGCGTTCATCATTCCAACATATTCCAC                                                      >  SRR4302535.959471/1‑94 (MQ=60)

                                         ACGTAAAGAGCCTTTCATCTTTTGGCGAAAGGATTAACCCTGATGTTTGGGGTCAATCAATTGCAATGCGTTCATCATTCCAACATATTCCACA                                                     <  SRR4302535.367337/94‑1 (MQ=60)

                                           GTAAAGAGCCTTTCATCTTTTGGCGAAAGGATTAACCCTGATGTTTGGGGTCAATCAATTGCAATGCGTTCATCATTCCAACATATTCCACAAT                                                   >  SRR4302535.3750903/1‑94 (MQ=60)

                                           GTAAAGAGCCTTTCATCTTTTGGCGAAAGGATTAACCCTGATGTTTGGGGTCAATCAATTGCAATGCGTTCATCATTCCAACATATTCCACAAT                                                   <  SRR4302535.3102730/94‑1 (MQ=60)

                                              AAGAGCCTTTCATCTTTTGGCGAAAGGATTAACCCTGATGTTTGGGGTCAATCAATTGCAATGCGTTCATCATTCCAACATATTCCACAATCGC                                                >  SRR4302535.194098/1‑94 (MQ=60)

                                                  GCCTTTCATCTTTTGGCGGAAGGATTAACCCTGATGGTTGGGGTCAATCAATTGCAATGCGTTCATCATTCCAACATATTCCACAATCGCTTTC                                            >  SRR4302535.2417763/1‑94 (MQ=60)

                                                  GCCTTTCATCTTTTGGCGAAAGGATTAACCCTGATGTTTGGGGTCAATCAATTGCAATGCGTTCATCATTCCAACATATTCCACAATCGCTTTC                                            >  SRR4302535.1731572/1‑94 (MQ=60)

                                                  GCCTTTCATCTTTTGGCGAAAGGATTAACCCTGATGTTTGGGGTCAATCAATTGCAATGCGTTCATCATTCCAACATATTCCACAATCGCTTTC                                            >  SRR4302535.4219799/1‑94 (MQ=60)

                                                   CCTTTCATCTTTTGGCGAAAGGATTAACCCTGATGTTTGGGGTCAATCAATTGCAATGCGTTCATCATTCCAACATATTCCACAATCGCTTTCA                                           >  SRR4302535.4128393/1‑94 (MQ=60)

                                                    CTTTCATCTTTTGGCGAAAGGATTAACCCTGATGTTTGGGGTCAATCAATTGCAATGCGTTCATCATTCCAACATATTCCACAATCGCTTTCAG                                          <  SRR4302535.1149506/94‑1 (MQ=60)

                                                     TTTCATCTTTTGGCGAAAGGATTAACCCTGATGTTTGGGGTTAATCAATTGCAATGCGTTCATCATTCCAACATATTCCACAATCGCTTTCAGG                                         >  SRR4302535.4092141/1‑94 (MQ=60)

                                                       TCATCTTTTGGCGAAAGGATTAACCCTGATGTTTGGGGTCAATCAATTGCAATGCGTTCATCATTCCAACATATTCCACAATCGCTTTCAGGAT                                       <  SRR4302535.1587041/94‑1 (MQ=60)

                                                        CATCTTTTGGCGAAAGGATTAACCCTGATGTTTGGGGTCAATCAATTGCAATGCGTTCATCATTCCAACATATTCCACAATCGCTTTCAGGATG                                      >  SRR4302535.5171/1‑94 (MQ=60)

                                                        CATCTTTTGGCGAAAGGATTAACCCTGATGTTTGGGGTCAATCAATTGCAATGCGTTCATCATTCCAACATATTCCACAATCGCTTTCAGGATG                                      >  SRR4302535.3688027/1‑94 (MQ=60)

                                                         ATCTTTTGGCGAAAGGATTAACCCTGATGTTTGGGGTCAATCAATTGCAATGCGTTCATCATTCCAACATATTCCACAATCGCTTTCAGGATGA                                     <  SRR4302535.4406170/94‑1 (MQ=60)

                                                         ATCTTTTGGCGAAAGGATTAACCCTGATGTTTGGGGTCAATCAATTGCAATGCGTTCATCATTCCAACATATTCCACAATCGCTTTCAGGATGA                                     >  SRR4302535.4605649/1‑94 (MQ=60)

                                                           CTTTTGGCGAAAGGATTAACCCTGATGTTTGGGGTCAATCAATTGCAATGCGTTCATCATTCCAACATATTCCACAATCGCTTTCAGGATGATA                                   <  SRR4302535.2143678/94‑1 (MQ=60)

                                                           CTTTTGGCGAAAGGATTAACCCTGATGTTTGGGGTCAATCAATTGCAATGCGTTCATCATTCCAACATATTCCACAATCGCTTTCAGGATGATA                                   >  SRR4302535.542348/1‑94 (MQ=60)

                                                           CTTTTGGCGAAAGGATTAACCCTGATGTTTGGGGTCAATCAATTGCAATGCGTTCATCATTCCAACATATTCCACAATCGCTTTCAGGATGATA                                   >  SRR4302535.2413310/1‑94 (MQ=60)

                                                           CTTTTGGCGAAAGGATTAACCCTGATGTTTGGGGTCAATCAATTGCAATGCGTTCATCATTCCAACATATTCCACAATCGCTTTCAGGATGACA                                   <  SRR4302535.743023/94‑1 (MQ=60)

                                                             TTTGGCGAAAGGATTAACCCTGATGTTTGGGGTCAATCAATTGCAATGCGTTCATCATTCCAACATATTCCACAATCGCTTTCAGGATGATAAT                                 <  SRR4302535.4511400/94‑1 (MQ=60)

                                                                GGCGAAAGGATTAACCCTGATGTTTGGGGTCAATCAATTGCAATGCGTTCATCATTCCAACATATTCCACAATCGCTTTCAGGATGATAATAGT                              <  SRR4302535.4197054/94‑1 (MQ=60)

                                                                GGCGAAAGGATTAACCCTGATGTTTGGAGTCAATCAATTGCAATGCGTTCATCATTCCAACATATTCCACAATCGCTTTCAGGATGATAATAGT                              <  SRR4302535.500937/94‑1 (MQ=60)

                                                                 GCGAAAGGATTAACCCTGATGTTTGGGGTCAATCAATTGCAATGCGTTCATCATTCCAACATATTCCACAATCGCTTTCAGGATGATAATAGTT                             <  SRR4302535.3876358/94‑1 (MQ=60)

                                                                  CGAAAGGATTAACCCTGATGTTTGGGGTCAATCAATTGCAATGCGTTCATCATTCCAACATATTCCACAATCGCTTTCAGGATGATAATAGTTT                            <  SRR4302535.3537663/94‑1 (MQ=60)

                                                                   GAAAGGATTAACCCTGATGTTTGGGGTCAATCAATTGCAATGCGTTCATCATTCCAACATATTCCACAATCGCTTTCAGGATGATAATAGTTTG                           <  SRR4302535.1319575/94‑1 (MQ=60)

                                                                   GAAAGGATTAACCCTGATGTTTGGGGTCAATCAATTGCAATGCGTTCATCATTCCAACATATTCCACAATCGCTTTCAGGATGATAATAGTTTG                           >  SRR4302535.2570288/1‑94 (MQ=60)

                                                                   GAAAGGATTAACCCTGATGTTTGGGGTCAATCAATTGCAATGCGTTCATCATTCCAACATATTCCACAATCGCTTTCAGGATGATAATAGTTTG                           >  SRR4302535.2570289/1‑94 (MQ=60)

                                                                    AAAGGATTAACCCTGATGTTTGGGGTCAATCAATTGCAATGCGTTCATCATTCCAACATATTCCACAATCGCTTTCAGGATGATAATAGTTTGT                          >  SRR4302535.4346361/1‑94 (MQ=60)

                                                                      AGGATTAACCCTGATGTTTGGGGTCAATCAATTGCAATGCGTTCATCATTCCAACATATTCCACAATCGCTTTCAGGATGATAATAGTTTGTAG                        <  SRR4302535.3263091/94‑1 (MQ=60)

                                                                      AGGATTAACCCTGATGTTTGGGGTCAATCAATTGCAATGCGTTCATCATTCCAACATATTCCACAATCGCTTTCAGGATGATAATAGTTTGTAG                        <  SRR4302535.667711/94‑1 (MQ=60)

                                                                         ATTAACCCTGATGTTTGGGGTCAATCAATTGCAATGCGTTCATCATTCCAACATATTCCACAATCGCTTTCAGGATGATAATAGTTTGTAGTTT                     >  SRR4302535.2367351/1‑94 (MQ=60)

                                                                           TAACCCTGATGTTTGGGGTCAATCAATTGCAATGCGTTCATCATTCCAACATATTCCACAATCGCTTTCAGGATGATAATAGTTTGTAGTTTTA                   <  SRR4302535.3960993/94‑1 (MQ=60)

                                                                            AACCCTGATGTTTGGGGTCAATCAATTGCAATGCGTTCATCATTCCAACATATTCCACAATCGCTTTCAGGATGATAATAGTTTGTAGTTTTAT                  >  SRR4302535.1623653/1‑94 (MQ=60)

                                                                            AACCCTGATGTTTGGGGTCAATCAATTGCAATGCGTTCATCATTCCAACATATTCCACAATCGCTTTCAGGATGATAATAGTTTGTAGTTTTAT                  >  SRR4302535.655225/1‑94 (MQ=60)

                                                                             ACCCTGATGTTTGGGGTCAATCAATTGCAATGCGTTCATCATTCCAACATATTCCACAATCGCTTTCAGGATGATAATAGTTTGTAGTTTTATA                 <  SRR4302535.2341657/94‑1 (MQ=60)

                                                                               CCTGATGTTTGGGGTCAATCAATTGCAATGCGTTCATCATTCCAACATATTCCACAATCGCTTTCAGGATGATAATAGTTTGTAGTTTTATATT               <  SRR4302535.1077665/94‑1 (MQ=60)

                                                                               CCTGATGTTTGCGGTCAATCAATTGCAATGCGTTCATCATTCCAACATATTCCACAATCGCTTTCAGGATGATAATAGTTTGTAGTTTTATATT               <  SRR4302535.1071114/94‑1 (MQ=60)

                                                                                CTGATGTTTGGGGTCAATCAATTGCAATGCGTTCATCATTCCAACATATTCCACAATCGCTTTCAGGATGATAATAGTTTGTAGTTTTATATTG              >  SRR4302535.2408928/1‑94 (MQ=60)

                                                                                CTGATGTTTGGGGTCAATCAATTGCAATGCGTTCATCATTCCAACATATTCCACAATCGCTTTCAGGATGATAATAGTTTGTAGTTTTATATTG              <  SRR4302535.3346375/94‑1 (MQ=60)

                                                                                 TGATGTTTGGGGTCAATCAATTGCAATGCGTTCATCATTCCAACATATTCCACAATCGCTTTCAGGATGATAATAGTTTGTAGTTTTATATTGA             >  SRR4302535.1068405/1‑94 (MQ=60)

                                                                                   ATGTTTGGGGTCAATCAATTGCAATGCGTTCATCATTCCAACATATTCCACAATCGCTTTCAGGATGATAATAGTTTGTAGTTTTATATTGACA           <  SRR4302535.4452183/94‑1 (MQ=60)

                                                                                   ATGTTTGGGGTCAATCAATTGCAATGCGTTCATCATTCCAACATATTCCACAATCGCTTTCAGGATGATAATAGTTTGTAGTTTTATATTGAAA           >  SRR4302535.1875831/1‑94 (MQ=60)

                                                                                   ATGTTTGGGGTCAATCAATTGCAATGCGTTCATCATTCCAACATATTCCACAATCGCTTTCAGGATGATAATAGTTTGTAGTTTTATATTGAAA           <  SRR4302535.4355582/94‑1 (MQ=60)

                                                                                   ATGTTTGGGGTCAATCAATTGCAATGCGTTCATCATTCCAACATATTCCACAATCGCTTTCAGGATGATAATAGTTTGTAGTTTTATATTGAAA           <  SRR4302535.3785008/94‑1 (MQ=60)

                                                                                    TGTTTGGGGTCAATCAATTGCAATGCGTTCATCATTCCAACATATTCCACAATCGCTTTCAGGATGATAATAGTTTGTAGTTTTATATTGAAAT          >  SRR4302535.2702239/1‑94 (MQ=60)

                                                                                    TGTTTGGGGTCAATCAATTGCAATGCGTTCATCATTCCAACATATTCCACAATCGCTTTCAGGATGATAATAGTTTGTAGTTTTATATTGAAAT          >  SRR4302535.2900773/1‑94 (MQ=60)

                                                                                    TGTTTGGGGTCAATCAATTGCAATGCGTTCATCATTCCAACATATTCCACAATCGCTTTCAGGATGATAATAGTTTGTAGTTTTATATTGAAAT          <  SRR4302535.3067094/94‑1 (MQ=60)

                                                                                      TTTGGGGTCAATCAATTGCAATGCGTTCATCATTCCAACATATTCCACAATCGCTTTCAGGATGATAATAGTTTGTAGTTTTATATTGAAATTC        <  SRR4302535.1751184/94‑1 (MQ=60)

                                                                                      TTTGGGGTCAATCAATTGCAATGCGTTCATCATTCCAACATATTCCACAATCGCTTTCAGGATGATAATAGTTTGTAGTTTTATATTGAAATTC        <  SRR4302535.3466336/94‑1 (MQ=60)

                                                                                       TTGGGGTCAATCAATTGCAATGCGTTCATCATTCCAACATATTCCACAATCGCTTTCAGGATGATAATAGTTTGTAGTTTTATATTGAAATTCA       <  SRR4302535.509869/94‑1 (MQ=60)

                                                                                            GTCAATCAATTGCAATGCGTTCATCATTCCAACATATTCCACAATCGCTTTCAGGATGATAATAGTTTGTAGTTTTATATTGAAATTCAGCGAT  <  SRR4302535.3049379/94‑1 (MQ=60)

TCGTATTCATGCAATTAATTTTAATCTGATAAGCTCATCTAACGTAAAGAGCCTTTCATCTTTTGGCGAAAGGATTAACCCTGATGTTTGGGGCCAATCAATTGCAATGCGTTCATCATTCCAACATATTCCACAATCGCTTTCAGGATGATAATAGTTTGTAGTTTTATATTGAAATTCAGCGAT  >  NC_000913/2107597‑2107782

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 26 ≤` `ATCG/ATCG` `< 31 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`