Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I2 R1	1873	183.1	9376148	98.2%	9207377	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,281,741	T→C	D76G (GAC→GGC)	yejK ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,281,741	0	T	C	99.2%	396.1 / NA	128	D76G (GAC→GGC)	yejK	hypothetical protein
Reads supporting (aligned to +/- strand): ref base T (0/0); major base C (66/61); minor base G (1/0); total (67/61)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 148 total aligned reads displayed.

AAAACAGCACGAATCCACCGTCAGCAAAAGGATATTTCGCTAATTCGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGTCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCAGTTCACTCTCTTCGCTAAACAAGCCGTAAGCTTTATTTTTGGCGCTATAG  >  NC_000913/2281652‑2281830

  aaCAGCACGAATCCACCGTCAGCAAAAGGATATTTCTCTAATTCGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCctct                                                                                      <  1:351311/93‑1 (MQ=255)

  aaCAGCACGAATCCACCGTCAGCAAAAGGATATTTCGCTAATTCGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCctct                                                                                      >  1:3738710/1‑93 (MQ=255)

  aaCAGCACGAATCCACCGTCAGCAAAAGGATATTTCGCTAATTCGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCctct                                                                                      <  1:1737305/93‑1 (MQ=255)

  aaCAGCACGAATCCACCGTCAGCAAAAGGATATTTCGCTAATTCGTCACGCAGGCGGCCGGCTGCCGCGCGGCTAAATGCCAGGAAGCCctct                                                                                      >  2:2478304/1‑93 (MQ=255)

   aCAGCACGAATCCACCGTCAGCAAAAGGATATTTCGCTAATTCGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCtt                                                                                     <  1:4051949/93‑1 (MQ=255)

   aCAGCACGAATCCACCGTCAGCAAAAGGATATTTCGCTAATTCGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCtt                                                                                     <  1:650691/93‑1 (MQ=255)

    cAGCACGAATCCACCGTCAGCAAAAGGATATTTCGCTAATTCGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTc                                                                                    <  1:1970434/93‑1 (MQ=255)

      gCACGAATCCACCGTCAGCAAAAGGATATTTCGCTAATTCGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGc                                                                                  >  2:2386364/1‑93 (MQ=255)

        aCGAATCCACCGTCAGCAAAAGGATATTTCGCTAATTCGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGccc                                                                                <  2:2726331/93‑1 (MQ=255)

        aCGAATCCACCGTCAGCAAAAGGATATTTCGCTAATTCGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGccc                                                                                >  2:447089/1‑93 (MQ=255)

         cGAATCCACCGTCAGCAAAAGGATATTTCGCTAATTCGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCt                                                                               >  1:4155341/1‑93 (MQ=255)

          gAATCCACCGTCAGCAAAAGGATATTTCGCTAATTCGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTg                                                                              >  2:3516313/1‑93 (MQ=255)

           aaTCCACCGTCAGCAAAAGGATATTTCGCTAATTCGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCTCTCTTCGCCCTgc                                                                             >  1:3833856/1‑92 (MQ=255)

           aaTCCACCGTCAGCAAAAGGATATTTCGCTAATTCGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGa                                                                             >  1:123916/1‑93 (MQ=255)

           aaTCCACCGTCAGCAAAAGGATATTTCGCTAATTCGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGa                                                                             >  1:3014498/1‑93 (MQ=255)

            aTCCACCGTCAGCAAAAGGATATTTCGCTAATTCGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGAc                                                                            <  2:638002/93‑1 (MQ=255)

             tCCACCGTCAGCAAAAGGATATTTCGCTAATTCGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACg                                                                           >  1:2232167/1‑93 (MQ=255)

             tCCACCGTCAGCAAAAGGATATTTCGCTAATTCGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACg                                                                           >  1:3633458/1‑93 (MQ=255)

                  cGTCAGCAAAAGGATATTTCGCTAATTCGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTa                                                                      <  2:4628112/93‑1 (MQ=255)

                   gTCAGCAAAAGGATATTTCGCTAATTCGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTaa                                                                     <  2:1752013/93‑1 (MQ=255)

                    tCAGCAAAAGGATATTTCGCTAATTCGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAg                                                                    <  1:3085213/93‑1 (MQ=255)

                     cAGCAAAAGGATATTTCGCTAATTCGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAgc                                                                   <  1:4587873/93‑1 (MQ=255)

                     cAGCAAAAGGATATTTCGCTAATTCGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAgc                                                                   >  2:1062596/1‑93 (MQ=255)

                       gCAAAAGGATATTTCGCTAATTCGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAgcgc                                                                 <  2:4344972/93‑1 (MQ=255)

                       gCAAAAGGATATTTCGCTAATTCGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAgcgc                                                                 >  1:1492245/1‑93 (MQ=255)

                        cAAAAGGATATTTCGCTAATTCGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCa                                                                >  1:478173/1‑93 (MQ=255)

                         aaaaGGATATTTCGCTAATTCGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAg                                                               <  1:2636654/93‑1 (MQ=255)

                          aaaGGATATTTCGCTAATTCGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGc                                                              >  1:1486189/1‑93 (MQ=255)

                            aGGATATTTCGCTAATTCGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGt                                                            >  2:3012146/1‑93 (MQ=255)

                            aGGATATTTCGCTAATTCGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGt                                                            <  2:496868/93‑1 (MQ=255)

                             ggATATTTCGCTAATTCGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTc                                                           >  1:665705/1‑93 (MQ=255)

                             ggATATTTCGCTAATTCGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTc                                                           >  1:2396852/1‑93 (MQ=255)

                             ggATATTTCGCTAATTCGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTc                                                           >  2:2669065/1‑93 (MQ=255)

                             ggATATTTCGCTAATTCGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTc                                                           >  1:4132186/1‑93 (MQ=255)

                             ggATATTTCGCTAATTCGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTc                                                           <  1:946229/93‑1 (MQ=255)

                              gATATTTCGCTAATTCGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCt                                                          >  2:404379/1‑93 (MQ=255)

                              gATATTTCGCTAATTCGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCt                                                          <  2:3281431/93‑1 (MQ=255)

                              gATATTTCGCTAATTCGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCt                                                          <  1:3202766/93‑1 (MQ=255)

                               atatTTCGCTAATTCGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTg                                                         <  1:3666889/93‑1 (MQ=255)

                               atatTTCGCTAATTCGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTg                                                         >  2:2540618/1‑93 (MQ=255)

                               atatTTCGCTAATTCGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTg                                                         >  2:2951216/1‑93 (MQ=255)

                               atatTTCGCTAATTCGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTg                                                         >  2:4157548/1‑93 (MQ=255)

                               atatTTCGCTAATTCGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTg                                                         >  1:1062576/1‑93 (MQ=255)

                               atatTTCGCTAATTCGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCACTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTg                                                         >  2:3307696/1‑93 (MQ=255)

                                tatTTCGCTAATTCGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTgc                                                        >  2:2650909/1‑93 (MQ=255)

                                tatTTCGCTAATTCGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTgc                                                        <  2:4653858/93‑1 (MQ=255)

                                tatTTCGCTAATTCGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCATCGCCCTGACGCTGTAAGCGCAGCGTCTgc                                                        >  2:3469400/1‑93 (MQ=255)

                                 atTTCGCTAATTCGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTgcg                                                       >  1:1154261/1‑93 (MQ=255)

                                 atTTCGCTAATTCGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTgcg                                                       <  1:3977075/93‑1 (MQ=255)

                                    tCGCTAATTCGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCa                                                    <  1:697319/93‑1 (MQ=255)

                                      gCTAATTCGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCAGt                                                  >  1:3316732/1‑93 (MQ=255)

                                         aaTTCGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCAGTTCa                                               <  2:1181436/93‑1 (MQ=255)

                                          aTTCGTCACGCAGGTGGCCGGTTGCCCCGCGGCTAAATGCCCGGAAGCCGTCTTCGCCCCAACGCTGTTCGCGCAGCCTCGGCGCCAGTTCat                                              >  2:666154/1‑92 (MQ=255)

                                          aTTCGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGGCCTCTTCGCCCTGACGATGTAAGCGCAGCGTCTGCGCCAGTTCaa                                              >  2:3100587/1‑92 (MQ=255)

                                                cACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCAGTTCACTCTCtt                                        >  1:3984162/1‑93 (MQ=255)

                                                cACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTCCGCCAGTTCACTCTCtt                                        >  1:2999664/1‑93 (MQ=255)

                                                  cGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCAGTTCACTCTCTTCg                                      >  2:850188/1‑93 (MQ=255)

                                                   gCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCAGTTCACTCTCTTCGc                                     >  2:3439740/1‑93 (MQ=255)

                                                   gCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCAGTTCACTCTCTTCGc                                     <  1:1642170/93‑1 (MQ=255)

                                                   gCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCAGTTCACTCTCTTCGc                                     <  1:4597545/93‑1 (MQ=255)

                                                    cAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCAGTTCACTCTCTTCGCt                                    <  2:2493448/93‑1 (MQ=255)

                                                     aGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCAGTTCACTCTCTTCGCTa                                   <  1:524763/93‑1 (MQ=255)

                                                      ggcggcCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCAGTTCACTCTCTTCGCTaa                                  >  2:2098163/1‑93 (MQ=255)

                                                          gcCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCAGTTCACTCTCTTCGCTAAACaa                              <  1:2397571/93‑1 (MQ=255)

                                                          gcCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCAGTTCACTCTCTTCGCTAAACaa                              >  2:4446227/1‑93 (MQ=255)

                                                          gcCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCAGTTCACTCTCTTCGCTAAACaa                              <  1:2268203/93‑1 (MQ=255)

                                                           ccGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCAGTTCACTCTCTTCGCTAAACAAg                             <  1:3785065/93‑1 (MQ=255)

                                                           ccGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCAGTTCACTCTCTTCGCTAAACAAg                             <  2:1181078/93‑1 (MQ=255)

                                                            cGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCAGTTCACTCTCTTCGCTAAACAAGc                            <  2:4226616/93‑1 (MQ=255)

                                                            cGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCAGTTCACTCTCTTCGCTAAACAAGc                            <  2:852474/93‑1 (MQ=255)

                                                             ggTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCAGTTCACTCTCTTCGCTAAACAAGcc                           <  1:853657/93‑1 (MQ=255)

                                                              gTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCAGTTCACTCTCTTCGCTAAACAAGCCg                          <  1:4050692/93‑1 (MQ=255)

                                                              gTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCAGTTCACTCTCTTCGCTAAACAAGCCg                          >  1:953057/1‑93 (MQ=255)

                                                              gTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCAGTTCACTCTCTTCGCTAAACAAGCCg                          <  2:2784393/93‑1 (MQ=255)

                                                                 gCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCAGTTCCCTCTCTTCGCTAAACAAGCCGTaa                       <  2:4669844/93‑1 (MQ=255)

                                                                 gCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCAGTTCACTCTCTTCGCTAAACAAGCCGTaa                       <  1:4331600/93‑1 (MQ=255)

                                                                 gCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCAGTTCACTCTCTTCGCTAAACAAGCCGTaa                       <  1:3840292/93‑1 (MQ=255)

                                                                 gCCGCGCGGCTAAATGCCAGGAAGCCCTCTCCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCGGTTCCCTCTCTTCGCTAAACAAGCCGTaa                       <  2:2136382/93‑1 (MQ=255)

                                                                   cgcgcgGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCAGTTCACTCTCTTCGCTAAACAAGCCGTAAGc                     >  2:177296/1‑93 (MQ=255)

                                                                     cgcgGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCAGTTCACTCTCTTCGCTAAACAAGCCGTAAGCtt                   <  2:2199027/93‑1 (MQ=255)

                                                                     cgcgGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCAGTTCACTCTCTTCGCTAAACAAGCCGTAAGCtt                   >  1:1155957/1‑93 (MQ=255)

                                                                        ggCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCAGTTCACTCTCTTCGCTAAACAAGCCGTAAGCTTTAt                <  2:2558071/93‑1 (MQ=255)

                                                                        ggCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCAGTTCACTCTCTTCGCTAAACAAGCCGTAAGCTTTAt                >  2:3938488/1‑93 (MQ=255)

                                                                          cTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCAGTTCACTCTCTTCGCTAAACAAGCCGTAAGCTTTAttt              >  2:788237/1‑93 (MQ=255)

                                                                           tAAATGCCAGGAGGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCAGTTCTCTCTCTTCGCTAAACAAGCCGTAAGCTTTAtttt             <  2:2096598/93‑1 (MQ=255)

                                                                           tAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCAGTTCACTCTCTTCGCTAAACAAGCCGTAAGCTTTAtttt             >  2:1256434/1‑93 (MQ=255)

                                                                            aaaTGCGCGGAAGCCCTCTTCCCCCTGACGCTTTAAGCGCAGCGTCTGCCCCAGTTCCCTCTCTCCGCTAAACAAGCCGTAAGCTTTAttttt            <  2:1689806/93‑1 (MQ=255)

                                                                            aaaTGCCAGGAACCCCCCTCCGACCTAACTTTGTAGTCGCAGCGTCTGCCCCAGTTCTCTCTTTTCGCTAAACAAGCCGTAAGCTTTAttttt            <  2:3576559/93‑1 (MQ=255)

                                                                            aaaCGCCAGGAGCCCCTCTTCGCCCTGACGCTGTAGGCGCGGCGTCTGCGCCAGTTCACTCTCTTCGCTAAACAAGCCGTAAGCTTTAttttt            <  2:1221701/93‑1 (MQ=255)

                                                                             aaTGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCAGTTCACTCTCTTCGCTAAACAAGCCGTAAGCTTTATTTTTg           >  1:4365330/1‑93 (MQ=255)

                                                                             aaTCCCAGGAAACCCTCTCCGCCCTGACGCTGTAAGCGAAGCGTCTGCGCCAGTTCACTCTCTTCGCTAAACAAGCCGTAAGCTTTATTTTTg           <  2:3797782/93‑1 (MQ=255)

                                                                              aTGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCAGTTCACTCTCTTCGCTAAACAAGCCGTAAGCTTTATTTTTgg          >  1:2595133/1‑93 (MQ=255)

                                                                              aTGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCAGGTCACTCTCTTCGCTAAACAAGCCGTAAGCTTTATTTTTgg          >  2:2419913/1‑93 (MQ=255)

                                                                                gCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCAGTTCACTCTCTTCGCTAAACAAGCCGTAAGCTTTATTTTTGgcg        >  2:735975/1‑93 (MQ=255)

                                                                                 ccAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCAGTTCACTCTCTTCGCTAAACAAGCCGTAAGCTTTATTTTTGgcgc       >  1:1632442/1‑93 (MQ=255)

                                                                                   cggAAGCCCTCTCCGCCCTGACGCTGTAAGCGCGGCGTCTGCGCCAGTTCACTCTCTTCGCTAAACAAGCCGTAAGCTTTATTTTTGGCGCta     <  1:812576/92‑1 (MQ=255)

                                                                                     gAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCAGTTCACTCTCTTCGCTAAACAAGCCGTAAGCTTTATTTTTGGCGCtata   >  2:1363522/1‑93 (MQ=255)

                                                                                     gAAGCCCTCTCCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCAGTTCACTCTCTTCGCTAAACAAGCCGTAAGCTTTATTTTTGGCGCtata   <  1:628697/93‑1 (MQ=255)

                                                                                      aaGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCAGTTCACTCTCTTCGCTAAACAAGCCGTAAGCTTTATTTTTGGCGCTATAg  >  1:1284950/1‑93 (MQ=255)

                                                                                      aaGCCCTCTCCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCAGTTCACTCTCTTCGCTAAACAAGCCGTAAGCTTTATTTTTGGCGCTATAg  <  1:3772374/93‑1 (MQ=255)

AAAACAGCACGAATCCACCGTCAGCAAAAGGATATTTCGCTAATTCGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGTCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCAGTTCACTCTCTTCGCTAAACAAGCCGTAAGCTTTATTTTTGGCGCTATAG  >  NC_000913/2281652‑2281830

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 20 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

GGCAAAACAGCACGAATCCACCGTCAGCAAAAGGATATTTCGCTAATTCGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGTCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCAGTTCACTCTCTTCGCTAAACAAGCCGTAAGCTTTATTTTTGGCGCTATAGAC  >  NC_000913/2281649‑2281832

GGCAAAACAGCACGAATCCACCGTCAGCAAAAGGATATTTCGCTAATTCGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCC                                                                                            <  SRR4302535.2326119/94‑1 (MQ=60)

GGCAAAACAGCACGAATCCACCGTCAGCAAAAGGATATTTCGCTAATTCGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCC                                                                                            >  SRR4302535.712199/1‑94 (MQ=60)

    AAACAGCACGAATCCACCGTCAGCAAAAGGATATTTCGCTAATTCGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCT                                                                                        >  SRR4302535.3764586/1‑94 (MQ=60)

     AACAGCACGAATCCACCGTCAGCAAAAGGATATTTCTCTAATTCGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTT                                                                                       <  SRR4302535.353732/94‑1 (MQ=60)

     AACAGCACGAATCCACCGTCAGCAAAAGGATATTTCGCTAATTCGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTT                                                                                       >  SRR4302535.4617554/1‑94 (MQ=60)

     AACAGCACGAATCCACCGTCAGCAAAAGGATATTTCGCTAATTCGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTT                                                                                       <  SRR4302535.1749863/94‑1 (MQ=60)

      ACAGCACGAATCCACCGTCAGCAAAAGGATATTTCGCTAATTCGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTC                                                                                      <  SRR4302535.3302344/94‑1 (MQ=60)

      ACAGCACGAATCCACCGTCAGCAAAAGGATATTTCGCTAATTCGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTC                                                                                      <  SRR4302535.4079689/94‑1 (MQ=60)

      ACAGCACGAATCCACCGTCAGCAAAAGGATATTTCGCTAATTCGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTC                                                                                      <  SRR4302535.655230/94‑1 (MQ=60)

       CAGCACGAATCCACCGTCAGCAAAAGGATATTTCGCTAATTCGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCG                                                                                     <  SRR4302535.1984628/94‑1 (MQ=60)

           ACGAATCCACCGTCAGCAAAAGGATATTTCGCTAATTCGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCT                                                                                 >  SRR4302535.4183788/1‑94 (MQ=60)

             GAATCCACCGTCAGCAAAAGGATATTTCGCTAATTCGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCTCTCTTCGCCCTGC                                                                               >  SRR4302535.3860294/1‑94 (MQ=60)

             GAATCCACCGTCAGCAAAAGGATATTTCGCTAATTCGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGA                                                                               >  SRR4302535.3035626/1‑94 (MQ=60)

             GAATCCACCGTCAGCAAAAGGATATTTCGCTAATTCGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGA                                                                               >  SRR4302535.124784/1‑94 (MQ=60)

               ATCCACCGTCAGCAAAAGGATATTTCGCTAATTCGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACG                                                                             >  SRR4302535.2248039/1‑94 (MQ=60)

               ATCCACCGTCAGCAAAAGGATATTTCGCTAATTCGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACG                                                                             >  SRR4302535.3658637/1‑94 (MQ=60)

               ATCCACCATCAGCAAAAGGATATTTCGCTAATTCGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACG                                                                             <  SRR4302535.4118032/94‑1 (MQ=60)

                 CCACCGTCAGCAAAAGGATATTTCGCTAATTCGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCT                                                                           >  SRR4302535.1170598/1‑94 (MQ=60)

                       TCAGCAAAAGGATATTTCGCTAATTCGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGC                                                                     <  SRR4302535.3106705/94‑1 (MQ=60)

                        CAGCAAAAGGATATTTCGCTAATTCGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCG                                                                    <  SRR4302535.4619112/94‑1 (MQ=60)

                         AGCAAAAGGATATTTCGCTAATTCGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGC                                                                   >  SRR4302535.1502965/1‑94 (MQ=60)

                          GCAAAAGGATATTTCGCTAATTCGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCA                                                                  >  SRR4302535.481526/1‑94 (MQ=60)

                           CAAAAGGATATTTCGCTAATTCGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAG                                                                 <  SRR4302535.745404/94‑1 (MQ=60)

                            AAAAGGATATTTCGCTAATTCGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGC                                                                >  SRR4302535.1496825/1‑94 (MQ=60)

                            AAAAGGATATTTCGCTAATTCGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGC                                                                <  SRR4302535.2655542/94‑1 (MQ=60)

                            AAAAGGATATTTCGCTAATTCGTCACGCAGGCGGCCGGGTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGCAAGCGCAGC                                                                >  SRR4302535.2117407/1‑94 (MQ=60)

                              AAGGATATTTCGCTAATTCGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGT                                                              <  SRR4302535.3102330/94‑1 (MQ=60)

                               AGGATATTTCGCTAATTCGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTC                                                             <  SRR4302535.3854081/94‑1 (MQ=60)

                               AGGATATTTCGCTAATTCGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTC                                                             >  SRR4302535.670349/1‑94 (MQ=60)

                               AGGATATTTCGCTAATTCGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTC                                                             >  SRR4302535.2414077/1‑94 (MQ=60)

                               AGGATATTTCGCTAATTCGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTC                                                             >  SRR4302535.4160458/1‑94 (MQ=60)

                                GGATATTTCGCTAATTCGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCT                                                            <  SRR4302535.952934/94‑1 (MQ=60)

                                 GATATTTCGCTAATTCGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTG                                                           >  SRR4302535.1070139/1‑94 (MQ=60)

                                 GATATTTCGCTAATTCGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTG                                                           <  SRR4302535.3224941/94‑1 (MQ=60)

                                  ATATTTCGCTAATTCGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGCAGCCCTATTCGCCCTGACGCTGTAAGCGCAGCGTCTGC                                                          >  SRR4302535.473716/1‑94 (MQ=60)

                                  ATATTTCGCTAATTCGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGC                                                          <  SRR4302535.3692280/94‑1 (MQ=60)

                                   TATTTCGCTAATTCGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCG                                                         >  SRR4302535.1162511/1‑94 (MQ=60)

                                    ATTTCGCTAATTCGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGC                                                        <  SRR4302535.4004246/94‑1 (MQ=60)

                                       TCGCTAATTCGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCAG                                                     <  SRR4302535.702212/94‑1 (MQ=60)

                                        CGCTAATTCGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCAGT                                                    >  SRR4302535.3339810/1‑94 (MQ=60)

                                          CTAATTCGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCTCCAGGTC                                                  >  SRR4302535.375177/1‑94 (MQ=60)

                                          CTAATTCGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCAGTTC                                                  >  SRR4302535.3538864/1‑94 (MQ=60)

                                           TAATTCGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCAGTTCA                                                 >  SRR4302535.1419452/1‑94 (MQ=60)

                                                  TCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCAGTTCACTCTCTT                                          >  SRR4302535.4011385/1‑94 (MQ=60)

                                                  TCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTCCGCCAGTTCACTCTCTT                                          >  SRR4302535.3020694/1‑94 (MQ=60)

                                                      GCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCAGTTCACTCTCTTCGCT                                      <  SRR4302535.4628822/94‑1 (MQ=60)

                                                      GCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCAGTTCACTCTCTTCGCT                                      <  SRR4302535.1654042/94‑1 (MQ=60)

                                                       CAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCAGTTCACTCTCTTCGCTA                                     >  SRR4302535.579348/1‑94 (MQ=60)

                                                        AGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCAGTTCACTCTCTTCGCTAA                                    <  SRR4302535.528431/94‑1 (MQ=60)

                                                           CGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCAGTTCACTCTCTTCGCTAAACA                                 <  SRR4302535.2491091/94‑1 (MQ=60)

                                                             GCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCAGTTCACTCTCTTCGCTAAACAAG                               <  SRR4302535.2414810/94‑1 (MQ=60)

                                                             GCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCAGTTCACTCTCTTCGCTAAACAAG                               <  SRR4302535.2284372/94‑1 (MQ=60)

                                                             GCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCAGTTCACTCTCTTCGCTAAACAAG                               <  SRR4302535.1509336/94‑1 (MQ=60)

                                                              CCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCAGTTCACTCTCTTCGCTAAACAAGC                              <  SRR4302535.3811176/94‑1 (MQ=60)

                                                                GGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCAGTTCACTCTCTTCGCTAAACAAGCCG                            >  SRR4302535.959811/1‑94 (MQ=60)

                                                                GGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCAGTTCACTCTCTTCGCTAAACAAGCCG                            <  SRR4302535.2305336/94‑1 (MQ=60)

                                                                GGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCAGTTCACTCTCTTCGCTAAACAAGCCG                            <  SRR4302535.859670/94‑1 (MQ=60)

                                                                 GTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCAGTTCACTCTCTTCGCTAAACAAGCCGT                           <  SRR4302535.4078422/94‑1 (MQ=60)

                                                                    GCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCAGTTCACTCTCTTCGCTAAACAAGCCGTAAG                        <  SRR4302535.4361223/94‑1 (MQ=60)

                                                                    GCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCAGTTCACTCTCTTCGCTAAACAAGCCGTAAG                        <  SRR4302535.3866756/94‑1 (MQ=60)

                                                                       GCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCAGTTCACTCTCTTCGCTAAACAAGCCGTAAGCTT                     >  SRR4302535.1164216/1‑94 (MQ=60)

                                                                           GGCTAAATGCCAGGAAGCCCTCTTCCCCCTGACGCTGTAAGCGCAGCGTCGGCGCCAGTTCCCTCTCTTCGCTAAACAAGCCGTAAGCTTTATT                 <  SRR4302535.1764438/94‑1 (MQ=60)

                                                                               AAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCAGTTCACTCTCTTCGCTAAACAAGCCGTAAGCTTTATTTTTG             >  SRR4302535.4395201/1‑94 (MQ=60)

                                                                                AATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCAGTTCACTCTCTTCGCTAAACAAGCCGTAAGCTTTATTTTTGG            >  SRR4302535.2613777/1‑94 (MQ=60)

                                                                                 AGGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCAGTTCACTCTCTTCGCTAAACAAGCCGTAAGCTTTATTTTTGGC           <  SRR4302535.99203/94‑1 (MQ=60)

                                                                                  TGCCAGGAAGCCCTCTTCGCCCTGACCCTGTACGCGCAGCGTCTGCGCCAGTTCCCTCTCTTCGCTAAACAAGCCGTAAGCTTTATTTTTGGCG          <  SRR4302535.4638768/94‑1 (MQ=60)

                                                                                   GCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCAGTTCACTCTCTTCGCTAAACAAGCCGTAAGCTTTATTTTTGGCGC         >  SRR4302535.3962477/1‑94 (MQ=60)

                                                                                   GCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCAGTTCACTCTCTTCGCTAAACAAGCCGTAAGCTTTATTTTTGGCGC         >  SRR4302535.1644238/1‑94 (MQ=60)

                                                                                    CCAGGAATCCCTCTTCTCCCTGACCCTGTAAGCGCGTCGTCGGCGCCAGTTCACTCTCTCCGCTAACCAAGCCGTAAGCTTTATTTTTGGCGCT        <  SRR4302535.56699/94‑1 (MQ=55)

                                                                                    CCAGGAAGCCTTCTTCGTCCTGACGCTGTAAGCGCAGCGTCTGCGCCAGTTCACTCTCTTCGCTAAACAAGCCGTAAGCTTTATTTTTGGCGCT        >  SRR4302535.2734474/1‑94 (MQ=60)

                                                                                    CCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCAGTTCACTCTCTTCGCTAAACAAGCCGTAAGCTTTATTTTTGGCGCT        >  SRR4302535.667849/1‑94 (MQ=60)

                                                                                    CCAGGAAGCCCTCTGCCCCCTGACGCTGTAAGCGCAGCGTCTGCGCCAGTTCACTCTCTTCGCTAAACAAGCCGTAAGCTTTATTTTTGGCGCT        <  SRR4302535.4045725/94‑1 (MQ=60)

                                                                                      CGGAAGCCCTCTCCGCCCTGACGCTGTAAGCGCGGCGTCTGCGCCAGTTCACTCTCTTCGCTAAACAAGCCGTAAGCTTTATTTTTGGCGCTAT      <  SRR4302535.818272/94‑1 (MQ=60)

                                                                                      AGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCAGTTCACTCTCTTCGCTAAACAAGCCGTAAGCTTTATTTTTGGCGCTAT      >  SRR4302535.937948/1‑94 (MQ=60)

                                                                                       GGAAGCCTTCTTCGCCCTGCCGTTGTAAGCGCGGCGTCTGCGCCATTTCACTCTCTTCGCTAAACAAGCCGTAAGCTTTATTTTTGGCGCTATA     <  SRR4302535.2171869/94‑1 (MQ=60)

                                                                                       GGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCAGTTCCCTCTCTTCGCTAAACAAGCCGTAAGCTTTATTTTTGGCGCTATA     <  SRR4302535.4320567/94‑1 (MQ=60)

                                                                                        GAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCAGTTCACTCTCTTCGCTAAACAAGCCGTAAGCTTTATTTTTGGCGCTATAG    <  SRR4302535.172454/94‑1 (MQ=60)

                                                                                        GAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCAGTTCACTCTCTTCGCTAAACAAGCCGTAAGCTTTATTTTTGGCGCTATAG    >  SRR4302535.1294106/1‑94 (MQ=60)

                                                                                        GAAGCCCTCTCCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCAGTTCACTCTCTTCGCTAAACAAGCCGTAAGCTTTATTTTTGGCGCTATAG    <  SRR4302535.633081/94‑1 (MQ=60)

                                                                                         AAGCCCTCTCCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCAGTTCACTCTCTTCGCTAAACAAGCCGTAAGCTTTATTTTTGGCGCTATAGA   <  SRR4302535.3798395/94‑1 (MQ=60)

                                                                                          AGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCAGTTCACTCTCTTCGCTAAACAAGCCGTAAGCTTTATTTTTGGCGCTATAGAC  >  SRR4302535.1098803/1‑94 (MQ=60)

                                                                                          AGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCAGTTCACTCTCTTCGCTAAACAAGCCGTAAGCTTTATTTTTGGCGCTATAGAC  >  SRR4302535.3602825/1‑94 (MQ=60)

GGCAAAACAGCACGAATCCACCGTCAGCAAAAGGATATTTCGCTAATTCGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGTCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCAGTTCACTCTCTTCGCTAAACAAGCCGTAAGCTTTATTTTTGGCGCTATAGAC  >  NC_000913/2281649‑2281832

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 2 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`