Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 1696 279.3 15874187 97.8% 15524954 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,281,741 T→C D76G (GAC→GGC)  yejK ← hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,281,7410TC99.5% 657.2 / ‑5.4 214D76G (GAC→GGC) yejKhypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (0/1);  new base C (131/82);  total (131/83)
Fisher's exact test for biased strand distribution p-value = 3.88e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.90e-01

Only 100 of 239 total aligned reads displayed.

CAAAACAGCACGAATCCACCGTCAGCAAAAGGATATTTCGCTAATTCGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGTCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCAGTTCACTCTCTTCGCTAAACAAGCCGTAAGCTTTATTTTTGGCGCTATA  >  NC_000913/2281651‑2281829
                                                                                          |                                                                                        
ccaaaCAGCAATAGTGCACCGTAATCAATAGGATATTTCGCTAATTAGTCACGCAGGCGGCGGGTTGCCGCGCGGCTAAATGCCAGGAAGccc                                                                                        <  2:4399305/91‑1 (MQ=255)
cAAAACAGCACGAATCCACCGTCAGCAAAAGGATATTTCGCTAATTCGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGccc                                                                                        <  1:433287/93‑1 (MQ=255)
  aaaCAGCACGAATCCACCGTCAGCAAAAGGATATTTCGCTAATTCGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCctc                                                                                      >  2:6905987/1‑93 (MQ=255)
   aaCAGCACGAATCCACCGTCAGCAAAAGGATATTTCGCTAATTCGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCctct                                                                                     <  1:7600670/93‑1 (MQ=255)
   aaCAGCACGAATCCACCGTCAGCAAAAGGATATTTCGCTAATTCGTCACGCAGGAGGCCGGCTGCCGCGCGGCTAAATGCCAGCAAGCCctct                                                                                     >  1:822668/1‑93 (MQ=255)
     cAGCACGAATCCACCGTCAGCAAAAGGATATTTCGCTAATTCGTCACTCAGGCGGCCTGTTGCCGCGCGGCCAAATGCCGGGCACGCctctcg                                                                                   >  1:5988095/1‑91 (MQ=255)
     cAGCACGAATCCACCGTCAGCAAAAGGATATTTCGCTAATTCGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTc                                                                                   <  2:2254004/93‑1 (MQ=255)
     cAGCACGAATCCACCGTCAGCAAAAGGATATTTCGCTAATTCGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTc                                                                                   <  1:995784/93‑1 (MQ=255)
      aGCACGAATCCACCGTCAGCAAAAGGATATTTCGCTAATTCGTCACGCAGGAGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCg                                                                                  >  2:7872968/1‑93 (MQ=255)
       gCACGAATCCACCGTCAGCAAAAGGATATTTCGCTAATTCGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGc                                                                                 >  1:4094346/1‑93 (MQ=255)
       gCACGAATCCACCGTCAGCAAAAGGATATTTCGCTAATTCGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGc                                                                                 >  1:2617040/1‑93 (MQ=255)
        cACGAATCCACCGTCAGCAAAAGGATATTTCGCTAATTCGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGcc                                                                                >  1:6967070/1‑93 (MQ=255)
         aCGAATCCACCGTCAGCAAAAGGATATTTCGCTAATTCGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGTCCTCCTCGccc                                                                               >  2:7657555/1‑93 (MQ=255)
         aCGAATCCACCGTCAGCAAAAGGATATTTCGCTAATTCGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGccc                                                                               >  2:4014167/1‑93 (MQ=255)
         aCGAATCCACCGTCAGCAAAAGGATATTTCGCTAATTCGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGccc                                                                               <  2:5345433/93‑1 (MQ=255)
         aCGAATCCACCGTCAGCAAAAGGATATTTCGCTAATTCGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGccc                                                                               >  2:2685934/1‑93 (MQ=255)
            aaTCCACCGTCAGCAAAAGGATATTTCGCTAATTCGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGa                                                                            <  1:3253224/93‑1 (MQ=255)
            aaTCCACCGTCAGCAAAAGGATATTTCGCTAATTCGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGa                                                                            <  1:4711852/93‑1 (MQ=255)
             aTCCACCGTCAGCAAAAGGATATATCGCTAATTCGTCACGCAGGCTGACGGTTGCCGCGCGGCTAAATGCCAGGACGCCCTCTTCGCCCTGAc                                                                           >  2:3789463/1‑93 (MQ=255)
               ccACCGTCAGCAAAAGGATATTTCGCTAATTCGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGc                                                                         <  1:2512041/93‑1 (MQ=255)
               ccACCGTCAGCAAAAGGATATTTCGCTAATTCGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGc                                                                         <  1:1940248/93‑1 (MQ=255)
                cACCGTCAGCAAAAGGATATTTCGCTAATTCGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCt                                                                        >  2:6689151/1‑93 (MQ=255)
                cACCGTCAGCAAAAGGATATTTCGCTAATTCGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCt                                                                        >  1:391490/1‑93 (MQ=255)
                 aCCGTCAGCAAAAGGATATTTCGCTAATTCGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTg                                                                       >  1:2009434/1‑93 (MQ=255)
                 aCCGTCAGCAAAAGGATATTTCGCTAATTCGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTg                                                                       <  1:2299206/93‑1 (MQ=255)
                      cAGCAAAAGGATATTTCGCTAATTCGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAgc                                                                  >  1:2182632/1‑93 (MQ=255)
                       aGCAAAAGGATATTTCGCTAATTCGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAgcg                                                                 >  2:4273818/1‑93 (MQ=255)
                         cAAAAGGATATTTCGCTAATTCGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCa                                                               >  2:805880/1‑93 (MQ=255)
                         cAAAAGGATATTTCGCTAATTCGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCa                                                               <  1:7844120/93‑1 (MQ=255)
                         cAAAAGGATATTTCGCTAATTCGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCa                                                               <  2:4391928/93‑1 (MQ=255)
                          aaaaGGATATTTCGCTAATTCGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAg                                                              >  1:7753438/1‑93 (MQ=255)
                               gATATTTCGCTAATTCGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCt                                                         <  1:492263/93‑1 (MQ=255)
                                atatTTCGCTAATTCGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTg                                                        <  2:3458894/93‑1 (MQ=255)
                                 tatTTCGCTAATTCGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTgc                                                       >  2:6256906/1‑93 (MQ=255)
                                 tatTTCGCTAATTCGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTgc                                                       >  1:6415998/1‑93 (MQ=255)
                                  atTTCGCTAATTCGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTgcg                                                      >  2:3026792/1‑93 (MQ=255)
                                  atTTCGCTAATTCGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTgcg                                                      >  2:721475/1‑93 (MQ=255)
                                   tttCGCCAATTCGTCACGCAGGCGGCCTGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTgcgc                                                     <  1:2738553/93‑1 (MQ=255)
                                      cGCTAATTCGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCAg                                                  <  1:1209879/93‑1 (MQ=255)
                                      cGCTAATTCGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCAg                                                  <  1:6547545/93‑1 (MQ=255)
                                         tAATTCGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCAGTTc                                               >  1:6490680/1‑93 (MQ=255)
                                         tAATTCGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCAGTTc                                               >  1:3128392/1‑93 (MQ=255)
                                            ttCGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCAGTTCAct                                            >  2:7346122/1‑93 (MQ=255)
                                            ttCGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCAGTTCAct                                            >  2:1571720/1‑93 (MQ=255)
                                            ttCGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCAGTTCAct                                            <  1:412858/93‑1 (MQ=255)
                                              cGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCAGTTCActct                                          <  1:732290/93‑1 (MQ=255)
                                               gTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCAGTTCActctc                                         >  2:3373178/1‑93 (MQ=255)
                                                tCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCAGTTCActctct                                        >  1:7560828/1‑93 (MQ=255)
                                                  ccGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCAGTTCACTCTCTTc                                      <  1:5539890/92‑1 (MQ=255)
                                                  aCGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCAGTTCACTCTCTTc                                      <  2:236475/93‑1 (MQ=255)
                                                    gCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCAGTTCACTCTCTTCGc                                    <  2:6378766/93‑1 (MQ=255)
                                                     cAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCAGTTCACTCTCTTCGCt                                   >  1:6697184/1‑93 (MQ=255)
                                                       ggcggcCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCAGTTCACTCTCTTCGCTaa                                 >  1:5179597/1‑93 (MQ=255)
                                                       ggcggcCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCAGTTCACTCTCTTCGCTaa                                 >  1:3287649/1‑93 (MQ=255)
                                                       ggcggcCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCAGTTCACTCTCTTCGCTaa                                 >  2:7429805/1‑93 (MQ=255)
                                                       ggcggcCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCAGTTCACTCTCTTCGCTaa                                 >  2:2760252/1‑93 (MQ=255)
                                                       ggcggcCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCAGTTCACTCTCTTCGCTaa                                 >  2:1079972/1‑93 (MQ=255)
                                                         cggcCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCATCTGCGCCAGTTCACTCTCTTCGCTAAAc                               <  2:4795712/93‑1 (MQ=255)
                                                           gcCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCAGTTCACTCTCTTCGCTAAACaa                             <  1:1746335/93‑1 (MQ=255)
                                                           gcCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCAGTTCACTCTCTTCGCTAAACaa                             <  1:7427010/93‑1 (MQ=255)
                                                           gcCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCAGTTCACTCTCTTCGCTAAACaa                             <  2:6051/93‑1 (MQ=255)
                                                           gcCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCAGTTCACTCTCTTCGCTAAACaa                             >  1:3558008/1‑93 (MQ=255)
                                                             cGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCAGTTCACTCTCTTCGCTAAACAAGc                           <  2:3875433/93‑1 (MQ=255)
                                                             cGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCAGTTCACTCTCTTCGCTAAACAAGc                           <  2:5433478/93‑1 (MQ=255)
                                                             cGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCAGTTCACTCTCTTCGCTAAACAAGc                           >  2:1279267/1‑93 (MQ=255)
                                                             cGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCAGTTCACTCTCTTCGCTAAACAAGc                           >  1:3822957/1‑93 (MQ=255)
                                                             cGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCAGTTCACTCTCTTCGCTAAACAAGc                           >  2:1788358/1‑93 (MQ=255)
                                                              ggTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCAGTTCACTCTCTTCGCTAAACAAGcc                          <  1:5333071/93‑1 (MQ=255)
                                                               gTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGCCGCTGTAAGCGCAGCGTCTGCGCCAGTTCACTCTCTTCGCTAAACAAGCCg                         <  1:2995985/93‑1 (MQ=255)
                                                               gTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCAGTTCACTCTCTTCGCTAAACAAGCCg                         <  2:6207723/93‑1 (MQ=255)
                                                               gTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCAGTTCACTCTCTTCGCTAAACAAGCCg                         >  2:4800524/1‑93 (MQ=255)
                                                               gTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCAGTTCACTCTCTTCGCTAAACAAGCCg                         >  1:7177091/1‑93 (MQ=255)
                                                                ttGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCAGTTCACTCTCTTCGCTAAACAAGCCGt                        >  2:1030706/1‑93 (MQ=255)
                                                                    cgcgcgGCTAAATGCCAGGAAGTCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCAGTTCACTCTCTTCGCTAAACAAGCCGTAAGc                    <  2:3674678/93‑1 (MQ=255)
                                                                       gcgGCTAAATGCCAGGAAGCCCTCTTCGCCCTTACGCTGTAAGCGCAGCGTCTGCGCCAGTTCACTCTCTTCGCTAAACAAGCTGCAAGCttt                 >  2:2340386/1‑93 (MQ=255)
                                                                       gcgGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCAGTTCACTCTCTTCGCTAAACAAGCCGTAAGCttt                 >  1:1579162/1‑93 (MQ=255)
                                                                       gcgGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCAGTTCACTCTCTTCGCTAAACAAGCCGTAAGCttt                 >  1:4440966/1‑93 (MQ=255)
                                                                       gcgGCTAAATGCCAGGAAGCCCTCTTCCCCCTGACCCTGTAAGCGCAGCGTCTGCGCCAGTTCCCTCTCTTCGCTAAACAAGCCGTAAGCttt                 <  1:2639268/93‑1 (MQ=255)
                                                                        cgGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCAGTTCACTCTCTTCGCTAAACAAGCCGTAAGCTTTa                <  2:5923351/93‑1 (MQ=255)
                                                                        cgGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCAGTTCACTCTCTTCGCTAAACAAGCCGTAAGCTTTa                >  1:397341/1‑93 (MQ=255)
                                                                        cgGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCAGTTCACTCTCTTCGCTAAACAAGCCGTAAGCTTTa                >  2:5505274/1‑93 (MQ=255)
                                                                        cgGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCAGTTCACTCTCTTCGCTAAACAAGCCGTAAGCTTTa                <  2:2141972/93‑1 (MQ=255)
                                                                          gCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCAGTTCACTCTCTTCGCTAAACAAGCCGTAAGCTTTAtt              <  2:1858127/93‑1 (MQ=255)
                                                                          gCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCAGTTCACTCTCTTCGCTAAACAAGCCGTAAGCTTTAtt              <  2:2509885/93‑1 (MQ=255)
                                                                          gCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCAGTTCACTCTCTTCGCTAAACAAGCCGTAAGCTTTAtt              <  2:6351841/93‑1 (MQ=255)
                                                                            tAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCAGTTCACTCTCTTCGCTAAACAAGCCGTAAGCTTTAtttt            >  2:1425140/1‑93 (MQ=255)
                                                                            tAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCAGTTCACTCTCTTCGCTAAACAAGCCGTAAGCTTTAtttt            >  1:571778/1‑93 (MQ=255)
                                                                              aaTGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCAGTTCACTCTCTTCGCTAAACAAGCCGTAAGCTTTATTTTTg          >  2:3279633/1‑93 (MQ=255)
                                                                                   cAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCAGTTCACTCTCTTCGCTAAACAAGCCGTAAGCTTTATTTTTGGCGCt     >  1:6853937/1‑93 (MQ=255)
                                                                                   cAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCAGTTCACTCTCTTCGCTAAACAAGCCGTAAGCTTTATTTTTGGCGCt     >  1:6201773/1‑93 (MQ=255)
                                                                                   cAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCAGTTCACTCTCTTCGCTAAACAAGCCGTAAGCTTTATTTTTGGCGCt     >  1:3667473/1‑93 (MQ=255)
                                                                                    aGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCAGTTCACTCTCTTCGCTAAACAAGCCGTAAGCTTTATTTTTTGCGCta    >  1:3595362/1‑93 (MQ=255)
                                                                                    aGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCAGTTCACTCTCTTCGCTAAACAAGCCGTAAGCTTTATTTTTGGCGCta    >  2:5768295/1‑93 (MQ=255)
                                                                                     ggAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCAGTTCACTCTCTTCGCTAAACAAGCCGTAAGCTTTATTTTTGGCGCtat   >  2:5554222/1‑93 (MQ=255)
                                                                                     ggAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCAGTTCACTCTCTTCGCTAAACAAGCCGTAAGCTTTATTTTTGGCGCtat   >  1:5275677/1‑93 (MQ=255)
                                                                                     ggAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCAGTTCACTCTCTTCGCTAAACAAGCCGTAAGCTTTATTTTTGGCGCtat   >  1:2389116/1‑93 (MQ=255)
                                                                                      gAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCAGTTCACTCTCTTCGCTAAACAAGCCGTAAGCTTTATTTTTGGCGCtata  <  2:4804510/93‑1 (MQ=255)
                                                                                      gAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCAGTTCACTCTCTTCGCTAAACAAGCCGTAAGCTTTATTTTTGGCGCtata  >  2:2215913/1‑93 (MQ=255)
                                                                                      gAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCAGTTCACTCTCTTCGCTAAACAAGCCGTAAGCTTTATTTTTGGCGCtata  >  2:5270986/1‑93 (MQ=255)
                                                                                      gAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCAGTTCACTCTCTTCGCTAAACAAGCCGTAAGCTTTATTTTTGGCGCtata  <  2:294464/93‑1 (MQ=255)
                                                                                          |                                                                                        
CAAAACAGCACGAATCCACCGTCAGCAAAAGGATATTTCGCTAATTCGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGTCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCAGTTCACTCTCTTCGCTAAACAAGCCGTAAGCTTTATTTTTGGCGCTATA  >  NC_000913/2281651‑2281829

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GGCAAAACAGCACGAATCCACCGTCAGCAAAAGGATATTTCGCTAATTCGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGTCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCAGTTCACTCTCTTCGCTAAACAAGCCGTAAGCTTTATTTTTGGCGCTATAGACCC  >  NC_000913/2281649‑2281834
                                                                                            |                                                                                             
GGCAAAACAGCACGAATCCACCGTCAGCAAAAGGATATTTCGCTAATTCGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCC                                                                                              <  SRR4302543.903347/94‑1 (MQ=60)
 GCAAAACAGCACGAATCCACCGTCAGCAAAAGGATATTTCGCTAATTCGTCACGCAGGCGGCCGGTTGCCGCNCGGCTAAATGCCAGGAAG‑CCT                                                                                            >  SRR4302543.6765140/1‑94 (MQ=60)
  CAAAACAGCACGAATCCACCGTCAGCAAAAGGATATTTCGCTAATTCGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCT                                                                                            >  SRR4302543.6559760/1‑94 (MQ=60)
  CAAAACAGCACGAATCCACCGTCAGCAAAAGGATATTTCGCTAATTCGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCT                                                                                            <  SRR4302543.3683414/94‑1 (MQ=60)
  CAAAACAGCACGAATCCACCGTCAGCAAAAGGATATTTCGCTAATTCGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCT                                                                                            <  SRR4302543.437349/94‑1 (MQ=60)
   AAAACAGCACGAATCCACCGTCAGCAAAAGGATATTTCGCTAATTCGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTC                                                                                           >  SRR4302543.7351829/1‑94 (MQ=60)
    AAACAGCACGAATCCACCGTCAGCAAAAGGATATTTCGCTAATTCGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCT                                                                                          >  SRR4302543.829919/1‑94 (MQ=60)
    AAACAGCACGAATCCACCGTCAGCAAAAGGATATTTCGCTAATTCGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCT                                                                                          >  SRR4302543.4165679/1‑94 (MQ=60)
    AAACAGCACGAATCCACCGTCAGCAAAAGGATATTTCGCTAATTCGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCT                                                                                          >  SRR4302543.366304/1‑94 (MQ=60)
    AAACAGCACGAATCCACCGTCAGCAAAAGGATATTTCGCTAATTCGTCACGCAGGAGGCCGGCTGCCGCGCGGCTAAATGCCAGCAAGCCCTCT                                                                                          >  SRR4302543.829921/1‑94 (MQ=60)
     AACAGCACGAATCCACCGTCAGCAAAAGGATATTTCGCTAATTCGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTT                                                                                         <  SRR4302543.7672857/94‑1 (MQ=60)
      ACAGCACGAATCCACCGTCAGCAAAAGGATATTTCGCTAATTCGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTC                                                                                        >  SRR4302543.6042972/1‑94 (MQ=60)
       CAGCACGAATCCACCGTCAGCAAAAGGATATTTCGCTAATTCGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCG                                                                                       <  SRR4302543.1004582/94‑1 (MQ=60)
        AGCACGAATCCACCGTCAGCAAAAGGATATTTCGCTAATTCGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGC                                                                                      >  SRR4302543.4244631/1‑94 (MQ=60)
        AGCACGAATCCACCGTCAGCAAAAGGATATTTCGCTAATTCGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGC                                                                                      >  SRR4302543.5289686/1‑94 (MQ=60)
        AGCACGAATCCACCGTCAGCAAAAGGATATTTCGCTAATTCGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGC                                                                                      >  SRR4302543.4131083/1‑94 (MQ=60)
        AGCACGAATCCACCGTCAGCAAAAGGATATTTCGCTAATTCGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGC                                                                                      >  SRR4302543.2640771/1‑94 (MQ=60)
         GCACGAATCCACCGTCAGCAAAAGGATATTTCGCTAATTCGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCC                                                                                     >  SRR4302543.3997219/1‑94 (MQ=60)
         GCACGAATCCACCGTCAGCAAAAGGATATTTCGCTAATTCGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCC                                                                                     >  SRR4302543.7032635/1‑94 (MQ=60)
          CACGAATCCACCGTCAGCAAAAGGATATTTCGCTAATTCGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAATCCCTCTTCGCCT                                                                                    >  SRR4302543.6905684/1‑94 (MQ=60)
          CACGAATCCACCGTCAGCAAAAGGATATTTCGCTAATTCGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCC                                                                                    >  SRR4302543.6683488/1‑94 (MQ=60)
              AATCCACCGTCAGCAAAAGGATATTTCGCTAATTCGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGAC                                                                                >  SRR4302543.4637536/1‑94 (MQ=60)
              AATCCACCGTCAGCAAAAGGATATTTCGCTAATTCGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGAC                                                                                <  SRR4302543.3282636/94‑1 (MQ=60)
              AATCCACCGTCAGCAAAAGGATATTTCGCTAATTCGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGAC                                                                                <  SRR4302543.4754911/94‑1 (MQ=60)
               ATCCACCGTCAGCAAAAGGATATTTCGCTAATTCGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACG                                                                               >  SRR4302543.7604760/1‑94 (MQ=60)
               ATCCACCGTCAGCAAAAGGATATTTCGCTAATTCGTCACGCAGGAGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACG                                                                               >  SRR4302543.34863/1‑94 (MQ=60)
                TCCACCGTCAGCAAAAGGATATTTCGCTAATTCGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGC                                                                              >  SRR4302543.6538916/1‑94 (MQ=60)
                 CCACCGTCAGCAAAAGGATATTTCGCTAATTCGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCT                                                                             >  SRR4302543.395105/1‑94 (MQ=60)
                 CCACCGTCAGCAAAAGGATATTTCGCTAATTCGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCT                                                                             <  SRR4302543.1957679/94‑1 (MQ=60)
                 CCACCGTCAGCAAAAGGATATTTCGCTAATTCGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCT                                                                             <  SRR4302543.2534842/94‑1 (MQ=60)
                  CACCGTCAGCAAAAGGATATTTCGCTAATTCGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTG                                                                            >  SRR4302543.2027491/1‑94 (MQ=60)
                   ACCGTCAGCAAAAGGATATTTCGCTAATTCGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGT                                                                           <  SRR4302543.2320162/94‑1 (MQ=60)
                     CGTCAGCAAAAGGATATTTCGCTAATTCGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAA                                                                         >  SRR4302543.4309696/1‑94 (MQ=60)
                     CGTCAGCAAAAGGATATTTCGCTAATTCGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAA                                                                         <  SRR4302543.4039073/94‑1 (MQ=60)
                     CGTCAGCAAAAGGATATTTCGCTAATTCGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAA                                                                         <  SRR4302543.7904965/94‑1 (MQ=60)
                     CGTCAGCAAAAGGATATTTCGCTAATTCGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAA                                                                         >  SRR4302543.2700664/1‑94 (MQ=60)
                     CGTCAGCAAAAGGATATTTCGCTAATTCGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAA                                                                         <  SRR4302543.2690174/94‑1 (MQ=60)
                      GTCAGCAAAAGGATATTTCGCTAATTCGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAG                                                                        <  SRR4302543.7881602/94‑1 (MQ=60)
                      GTCAGCAAAAGGATATTTCGCTAATTCGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAG                                                                        >  SRR4302543.4450535/1‑94 (MQ=60)
                       TCAGCAAAAGGATATTTCGCTAATTCGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGC                                                                       <  SRR4302543.5186882/94‑1 (MQ=60)
                       TCAGCAAAAGGATATTTCGCTAATTCGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGC                                                                       >  SRR4302543.2202198/1‑94 (MQ=60)
                           CAAAAGGATATTTCGCTAATTCGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAG                                                                   <  SRR4302543.7918056/94‑1 (MQ=60)
                           CAAAAGGATATTTCGCTAATTCGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAG                                                                   >  SRR4302543.2402390/1‑94 (MQ=60)
                           CAAAAGGATATTTCGCTAATTCGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAG                                                                   >  SRR4302543.7826642/1‑94 (MQ=60)
                            AAAAGGATATTTCGCTAATTCGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGC                                                                  <  SRR4302543.972504/94‑1 (MQ=60)
                              AAGGATATTTCGCTAATTCGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGT                                                                >  SRR4302543.3947018/1‑94 (MQ=60)
                                GGATATTTCGCTAATTCGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCT                                                              >  SRR4302543.2075747/1‑94 (MQ=60)
                                 GATATTTCGCTAATTCGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTG                                                             <  SRR4302543.5460221/94‑1 (MQ=60)
                                 GATATTTCGCTAATTCGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTG                                                             >  SRR4302543.7754377/1‑94 (MQ=60)
                                 GATATTTCGCTAATTCGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTG                                                             <  SRR4302543.496810/94‑1 (MQ=60)
                                  ATATTTCGCTAATTCGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGC                                                            >  SRR4302543.6476438/1‑94 (MQ=60)
                                     TTTCGCCAATTCGTCACGCAGGCGGCCTGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCC                                                         <  SRR4302543.2763466/94‑1 (MQ=60)
                                      TTCGCTAATTCGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCA                                                        >  SRR4302543.255717/1‑94 (MQ=60)
                                        TGCTAATTCGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCAGT                                                      <  SRR4302543.5376906/94‑1 (MQ=60)
                                        CGCTAATTCGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCAGT                                                      <  SRR4302543.6609171/94‑1 (MQ=60)
                                        CGCTAATTCGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCAGT                                                      <  SRR4302543.1220765/94‑1 (MQ=60)
                                        CGCTAATTCGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCAGT                                                      >  SRR4302543.1897582/1‑94 (MQ=60)
                                         GCTAATTCGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCAGTT                                                     <  SRR4302543.888058/94‑1 (MQ=60)
                                          CTAATTCGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCAGTTC                                                    >  SRR4302543.3156533/1‑94 (MQ=60)
                                          CTAATTCGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCAGTTC                                                    >  SRR4302543.6551826/1‑94 (MQ=60)
                                          CTAATTCGTCACGCAGGAGGCCGGTTGCCGCGCGGCTAAATGCAAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGGCAGTTC                                                    >  SRR4302543.3156532/1‑94 (MQ=60)
                                            AATTCGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCAGTTCAC                                                  >  SRR4302543.7233629/1‑94 (MQ=60)
                                            AATTCGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCAGTTCAC                                                  >  SRR4302543.3450480/1‑94 (MQ=60)
                                              TTCGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCAGTTCACTC                                                <  SRR4302543.416705/94‑1 (MQ=60)
                                                CGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCAGTTCACTCTC                                              <  SRR4302543.738853/94‑1 (MQ=60)
                                                CGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCAGTTCACTCTC                                              >  SRR4302543.2001535/1‑94 (MQ=60)
                                                 GTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCAGTTCACTCTCT                                             >  SRR4302543.7632799/1‑94 (MQ=60)
                                                 GTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCAGTTCACTCTCT                                             >  SRR4302543.7004687/1‑94 (MQ=60)
                                                 GTCACGCAGGCGGCAGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCAGTTCACTCTCT                                             >  SRR4302543.5274855/1‑94 (MQ=60)
                                                  TCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCAGTTCACTCTCTT                                            >  SRR4302543.3116447/1‑94 (MQ=60)
                                                  TCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCAGTTCACTCTCTT                                            >  SRR4302543.6504040/1‑94 (MQ=60)
                                                   CACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCAGTTCACTCTCTTC                                           >  SRR4302543.2513092/1‑94 (MQ=60)
                                                    CCGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCAGTTCACTCTCTTCG                                          <  SRR4302543.5590263/94‑1 (MQ=60)
                                                    ACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCAGTTCACTCTCTTCG                                          >  SRR4302543.6459532/1‑94 (MQ=60)
                                                      GCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCAGTTCACTCTCTTCGCT                                        >  SRR4302543.6760393/1‑94 (MQ=60)
                                                      GCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCAGTTCACTCTCTTCGCT                                        >  SRR4302543.6760394/1‑94 (MQ=60)
                                                        AGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCAGTTCACTCTCTTCGCTAA                                      >  SRR4302543.5226351/1‑94 (MQ=60)
                                                        AGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCAGTTCACTCTCTTCGCTAA                                      <  SRR4302543.2291609/94‑1 (MQ=60)
                                                        AGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCAGTTCACTCTCTTCGCTAA                                      >  SRR4302543.3317318/1‑94 (MQ=60)
                                                           CGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCAGTTCACTCTCTTCGCTAAACA                                   <  SRR4302543.6047558/94‑1 (MQ=60)
                                                            GGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCAGTTCACTCTCTTCGCTAAACAA                                  >  SRR4302543.3589526/1‑94 (MQ=60)
                                                             GCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCAGTTCACTCTCTTCGCTAAACAAG                                 <  SRR4302543.1761929/94‑1 (MQ=60)
                                                             GCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCAGTTCACTCTCTTCGCTAAACAAG                                 <  SRR4302543.26536/94‑1 (MQ=60)
                                                             GCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCAGTTCACTCTCTTCGCTAAACAAG                                 <  SRR4302543.7498224/94‑1 (MQ=60)
                                                              CCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCAGTTCACTCTCTTCGCTAAACAAGC                                >  SRR4302543.3856676/1‑94 (MQ=60)
                                                                GGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCAGTTCACTCTCTTCGCTAAACAAGCCG                              >  SRR4302543.7246762/1‑94 (MQ=60)
                                                                GGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCAGTTCACTCTCTTCGCTAAACAAGCCG                              <  SRR4302543.5381301/94‑1 (MQ=60)
                                                                 GTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGCCGCTGTAAGCGCAGCGTCTGCGCCAGTTCACTCTCTTCGCTAAACAAGCCGT                             <  SRR4302543.3023168/94‑1 (MQ=60)
                                                                 GTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCAGTTCACTCTCTTCGCTAAACAAGCCGT                             <  SRR4302543.1653195/94‑1 (MQ=60)
                                                                      CGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCAGTTCACTCTCTTCGCTAAACAAGCCGTAAGCT                        >  SRR4302543.5468708/1‑94 (MQ=60)
                                                                      CGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCAGTTCACTCTCTTCGCTAAACAAGCCGTAAGCT                        <  SRR4302543.3285154/94‑1 (MQ=60)
                                                                        CGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCAGTTCACTCTCTTCGCTAAACAAGCCGTAAGCTTT                      >  SRR4302543.5713448/1‑94 (MQ=60)
                                                                        CGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCAGTTCACTCTCTTCGCTAAACAAGCCGTAAGCTTT                      >  SRR4302543.4481181/1‑94 (MQ=60)
                                                                        CGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCAGTTCACTCTCTTCGCTAAACAAGCCGTAAGCTTT                      >  SRR4302543.1593257/1‑94 (MQ=60)
                                                                         GCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCAGTTCACTCTCTTCGCTAAACAAGCCGTAAGCTTTA                     >  SRR4302543.401013/1‑94 (MQ=60)
                                                                         GCGGCTAAATGCCAGGAAGCCCTCTTCCCCCTGACCCTGTAAGCGCAGCGTCTGCGCCAGTTCCCTCTCTTCGCTAAACAAGCCGTAAGCTTTA                     <  SRR4302543.2663172/94‑1 (MQ=60)
                                                                          CGGATAAATGCCAGAAAGCCCTCTCCCCCCTGACGCTGAAACCCCAGCGTCTGCGCCAGTTCACTCTCTTCGCTAAACAAGCCGTAAGCTTTAT                    <  SRR4302543.4549026/94‑1 (MQ=60)
                                                                          CGCCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCAGTTCACTCTCTTCGCTAAACAAGCCGTAAGCTTTAT                    <  SRR4302543.1847875/94‑1 (MQ=60)
                                                                          CGACTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCAGTTCACTCTCTTCGCTAAACAAGCCGTAAGCTTTAT                    <  SRR4302543.2439824/94‑1 (MQ=60)
                                                                           GGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCAGTTCACTCTCTTCGCTAAACAAGCCGTAAGCTTTATT                   >  SRR4302543.7361050/1‑94 (MQ=60)
                                                                             CTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCAGTTCACTCTCTTCGCTAAACAAGCCGTAAGCTTTATTTT                 >  SRR4302543.577014/1‑94 (MQ=60)
                                                                                AATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCAGTTCACTCTCTTCGCTAAACAAGCCGTAAGCTTTATTTTTGG              <  SRR4302543.399293/94‑1 (MQ=60)
                                                                                  TGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCAGTTCACTCTCTTCGCTAAACAAGCCGTAAGCTTTATTTTTGGCG            <  SRR4302543.4962321/94‑1 (MQ=60)
                                                                                    CCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCAGTTCACTCTCTTCGCTAAACAAGCCGTAAGCTTTATTTTTGGCGCT          >  SRR4302543.6918574/1‑94 (MQ=60)
                                                                                    CCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCAGTTCACTCTCTTCGCTAAACAAGCCGTAAGCTTTATTTTTGGCGCT          >  SRR4302543.6260344/1‑94 (MQ=60)
                                                                                    CCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCAGTTCACTCTCTTCGCTAAACAAGCCGTAAGCTTTATTTTTGGCGCT          >  SRR4302543.3699678/1‑94 (MQ=60)
                                                                                    CCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCAGTTCACTCTCTTCGCTAAACAAGCCGTAAGCTTTATTTTTGGCGCT          <  SRR4302543.1545796/94‑1 (MQ=60)
                                                                                    CCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCAGTTCACTCTCTTCGCTAAACAAGCCGTAAGCTTTATTTTTGGCGCT          <  SRR4302543.2309426/94‑1 (MQ=60)
                                                                                     CAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCAGTTCACTCTCTTCGCTAAACAAGCCGTAAGCTTTATTTTTTGCGCTA         >  SRR4302543.3627095/1‑94 (MQ=60)
                                                                                     CAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCAGTTCACTCTCTTCGCTAAACAAGCCGCAAGCTTTATTTTTAGCGCTA         >  SRR4302543.3627094/1‑94 (MQ=60)
                                                                                     CAGGAAGCCCTCTTCCCCCTGACGCTGTAAGCGCAGCGTCTGCGCCAGTTCACTCTCTTCGCTAAACAAGCCGTAAGCTTTATTTTTGGCGCTA         <  SRR4302543.2765342/94‑1 (MQ=60)
                                                                                      AGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCAGTTCACTCTCTTGGCTAAACAAGCCGTAAGCTTTATTTTTCGCGCTCT        >  SRR4302543.7427540/1‑94 (MQ=60)
                                                                                      AGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCAGTTCACTCTCTTCGCTAAACAAGCCGTAAGCTTTATTTTTGGCGCTAT        >  SRR4302543.3757132/1‑94 (MQ=60)
                                                                                      AGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCAGTTCACTCTCTTCGCTAAACAAGCCGTAAGCTTTATTTTTGGCGCTAT        >  SRR4302543.5323273/1‑94 (MQ=60)
                                                                                      AGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCAGTTCACTCTCTTCGCTAAACAAGCCGTAAGCTTTATTTTTGGCGCTAT        >  SRR4302543.5790922/1‑94 (MQ=60)
                                                                                      AGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCAGTTCACTCTCTTCGCTAAACAAGCCGTAAGCTTTATTTTTGGCGCTAT        >  SRR4302543.2410744/1‑94 (MQ=60)
                                                                                       GGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCAGTTCACTCTCTTCGCTAAACAAGCCGTAAGCTTTATTTTTGGCGCTATA       <  SRR4302543.3263861/94‑1 (MQ=60)
                                                                                       GGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCAGTTCACTCTCTTCGCTAAACAAGCCGTAAGCTTTATTTTTGGCGCTATA       >  SRR4302543.7645720/1‑94 (MQ=60)
                                                                                       GGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCAGTTCACTCTCTTCGCTAAACAAGCCGTAAGCTTTATTTTTGGCGCTATA       >  SRR4302543.75101/1‑94 (MQ=60)
                                                                                         AAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCAGTTCACTCTCTTCGCTAAACAAGCCGTAAGCTTTATTTTTGGCGCTATAGA     >  SRR4302543.3886783/1‑94 (MQ=60)
                                                                                          AGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCAGTTCACTCTCTTCGCTAAACAAGCCGTAAGCTTTATTTTTGGCGCTATAGAC    <  SRR4302543.996176/94‑1 (MQ=60)
                                                                                          AGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCAGTTCACTCTCTTCGCTAAACAAGCCGTAAGCTTTATTTTTGGCGCTATAGAC    >  SRR4302543.2251566/1‑94 (MQ=60)
                                                                                          AGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCAGTTCACTCTCTTCGCTAAACAAGCCGTAAGCTTTATTTTTGGCGCTATAGAC    <  SRR4302543.7566035/94‑1 (MQ=60)
                                                                                          AGCCCTCTTCCCCCTGACGCTGTAAGCGCAGCGTCTGCGCCAGTTCACTCTCTTCGCTAAACAAGCCGTAAGCTTTATTTTTGGCGCTATAGAC    <  SRR4302543.2661097/94‑1 (MQ=60)
                                                                                            CCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCAGTTCACTCTCTTCGCTAAACAAGCCGTAAGCTTTATTTTTGGCGCTATAGACCC  >  SRR4302543.916955/1‑94 (MQ=60)
                                                                                            |                                                                                             
GGCAAAACAGCACGAATCCACCGTCAGCAAAAGGATATTTCGCTAATTCGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGTCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCAGTTCACTCTCTTCGCTAAACAAGCCGTAAGCTTTATTTTTGGCGCTATAGACCC  >  NC_000913/2281649‑2281834

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑