Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I2 R1	1873	183.1	9376148	98.2%	9207377	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,940,536	T→C	A18A (GCA→GCG)	gcvA ←	GcvA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,940,536	0	T	C	99.4%	532.3 / NA	171	A18A (GCA→GCG)	gcvA	GcvA
Reads supporting (aligned to +/- strand): ref base T (0/1); new base C (78/92); total (78/93)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 185 total aligned reads displayed.

AAAAAATCCTCAAGAGACTTGATTTGATGACTTACTGCGGCTTGGGTCACAAAAAGCTCTTCTGCTGCGCGAGTGAAACTTAAATGGCGTGCTGCGGCATCAAAAACTCGTAAGGCATTTAGCGGTGGTAATCGTTTAGACATGGCTATTAAACTTTGATGTTAAATGAATTTAACAAT  >  NC_000913/2940447‑2940625

aaaaaaTCCTCAAGAGACTTGATTTGATGACTTACTGCGGCTTGGGTCACAAAAAGCTCTTCTGCTGCGCGAGTGAAACTTAAATGGCGcgct                                                                                        >  1:4129378/1‑93 (MQ=255)

  aaaaTCCTCAAGAGACTTGATTTGATGACTTACTGCGGCTTGGGTCACAAAAAGCTCTTCTGCTGCGCGAGTGAAACTTAAATGGCGcgctgc                                                                                      >  1:1961040/1‑93 (MQ=255)

  aaaaTCCTCAAGAGACTTGATTTGATGACTTACTGCGGCTTGGGTCACAAAAAGCTCTTCTGCTGCGCGAGTGAAACTTAAATGGCGcgctgc                                                                                      <  1:3700842/93‑1 (MQ=255)

   aaaTCCTCAAGAGACTTGATTTGATGACTTACTGCGGCTTGGGTCACAAAAAGCTCTTCTGCTGCGCGAGTGAAACTTAAATGGCGCGCTGCg                                                                                     >  1:3089067/1‑93 (MQ=255)

    aaTCCTCAAGAGACTTGATTTGATGACTTACTGCGGCTTGGGTCACAAAAAGCTCTTCTGCTGCGCGAGTGAAACTTAAATGGCGCGCTGCgg                                                                                    >  1:711779/1‑93 (MQ=255)

    aaTCCTCAAGAGACTTGATTTGATGACTTACTGCGGCTTGGGTCACAAAAAGCTCTTCTGCTGCGCGAGGGAAACTTAAATGGCGCGCTGCgg                                                                                    >  2:2262719/1‑93 (MQ=255)

     aTCCTCAAGAGACTTGATTTGATGACTTACTGCGGCTTGGGTCACAAAAAGCTCTTCTGCTGCGCGAGTGAAACTTAAATGGCGCGCTGCGGc                                                                                   >  1:147860/1‑93 (MQ=255)

      tCCTCAAGAGACTTGATTTGATGACTTACTGCGGCTTGGGTCACAAAAAGCTCTTCTGCTGCGCGAGTGAAACTTAAATGGCGCGCTGCGGCa                                                                                  <  2:2013481/93‑1 (MQ=255)

      tCCTCAAGAGACTTGATTTGATGACTTACTGCGGCTTGGGTCACAAAAAGCTCTTCTGCTGCGCGAGTGAAACTTAAATGGCGCGCTGCGGCa                                                                                  <  2:1718588/93‑1 (MQ=255)

      tCCTCAAGAGACTTGATTTGATGACTTACTGCGGCTTGGGTCACAAAAAGCTCTTCTGCTGCGCGAGTGAAACTTAAATGGCGCGCTGCGGCa                                                                                  <  1:4207737/93‑1 (MQ=255)

         tCAAGAGACTTGATTTGATGACTTACTGCGGCTTGGGTCACAAAAAGCTCTTCTGCTGCGCGAGTGAAACTTAAATGGCGCGCTGCGGCATCa                                                                               <  1:992938/93‑1 (MQ=255)

          cAAGAGACTTGATTTGATGACTTACTGCGGCTTGGGTCACAAAAAGCTCTTCTGCTGCGCGAGTGAAACTTAAATGGCGCGCTGCGGCATcta                                                                              >  1:1516039/1‑91 (MQ=255)

          cAAGAGACTTGATTTGATGACTTACTGCGGCTTGGGTCACAAAAAGCTCTTCTGCTGCGCGAGTGAAACTTAAATGGCGCGCTGCGGCATCaa                                                                              >  1:347510/1‑93 (MQ=255)

             gagaCTTGATTTGATGACTTACTGCGGCTTGGGTCACAAAAAGCTCTTCTGCTGCGCGAGTGAAACTTAAATGGCGCGCTGCGGCATCaaaaa                                                                           <  1:1032747/93‑1 (MQ=255)

             gagaCTTGATTTGATGACTTACTGCGGCTTGGGTCACAAAAAGCTCTTCTGCTGCGCGAGTGAAACTTAAATGGCGCGCTGCGGCATCaaaaa                                                                           <  2:1762079/93‑1 (MQ=255)

             gagaCTTGATTTGATGACTTACTGCGGCTTGGGTCACAAAAAGCTCTTCTGCTGCGCGAGTGAAACTTAAATGGCGCGCTGCGGCATCaaaaa                                                                           >  2:504569/1‑93 (MQ=255)

               gaCTTGATTTGATGACTTACTGCGGCTTGGGTCACAAAAAGCTCTTCTGCTGCGCGAGTGAAACTTAAATGGCGCGCTTCGGCATCAAAAACt                                                                         >  1:2106433/1‑93 (MQ=255)

                aCTTGATTTGATGACTTACTGCGGCTTGGGTCACAAAAAGCTCTTCTGCTGCGCGAGTGAAACTTAAATGGCGCGCTGCGGCATCAAAAACTc                                                                        >  2:2518610/1‑93 (MQ=255)

                 cTTGATTTGATGACTTACTGCGGCTTGGGTCACAAAAAGCTCTTCTGCTGCGCGAGTGAAACTTAAATGGCGCGCTGCGGCATCAAAAACTCg                                                                       <  2:2493510/93‑1 (MQ=255)

                 cTTGATTTGATGACTTACTGCGGCTTGGGTCACAAAAAGCTCTTCTGCTGCGCGAGTGAAACTTAAATGGCGCGCTGCGGCATCAAAAACTCg                                                                       >  1:3510548/1‑93 (MQ=255)

                  ttgatttgatGACTTACTGCGGCTTGGGTCACAAAAAGCTCTTCTGCTGCGCGAGTGAAACTTAAATGGCGCGCTGCGGCATCAAAAACTCGt                                                                      >  1:3404387/1‑93 (MQ=255)

                   tgatttgatGACTTACTGCGGCTTGGGTCACAAAAAGCTCTTCTGCTGCGCGAGTGAAACTTAAATGGCGCGCTGCGGCATCAAAAACTCGTa                                                                     <  2:3184411/93‑1 (MQ=255)

                    gatttgatGACTTACTGCGGCTTGGGTCACAAAAAGCTCTTCTGCTGCGCGAGTGAAACTTAAATGGCGCGCTGCGGCATCAAAAACTCGTaa                                                                    >  1:1260210/1‑93 (MQ=255)

                    gatttgatGACTTACTGCGGCTTGGGTCACAAAAAGCTCTTCTGCTGCGCGAGTGAAACTTAAATGGCGCGCTGCGGCATCAAAAACTCGTaa                                                                    <  1:1474/93‑1 (MQ=255)

                    gatttgatGACTTACTGCGGCTTGGGTCACAAAAAGCTCTTCTGCTGCGCGAGCGAAACTTAAATGGCGCGCTGCGGCATCAAAAACTCGTaa                                                                    >  2:1552762/1‑93 (MQ=255)

                      tttgatGACTTACTGCGGCTTGGGTCACAAAAAGCTCTTCTGCTGCGCGAGTGAAACTTAAATGGCGCGCTGCGGCATCAAAAACTCGTAAgg                                                                  <  1:272142/93‑1 (MQ=255)

                       ttgatGACTTACTGCGGCTTGGGTCACAAAAAGCTCTTCTGCTGCGCGAGTGAAACTTAAATGGCGCGCTGCGGCATCAAAAACTCGTAAGGc                                                                 <  1:2547690/93‑1 (MQ=255)

                       ttgatGACTTACTGCGGCTTGGGTCACAAAAAGCTCTTCTGCTGCGCGAGTGAAACTTAAATGGCGCGCTGCGGCATCAAAAACTCGTAAGGc                                                                 >  1:4334529/1‑93 (MQ=255)

                         gatgaCTTACTGCGGCTTGGGTCACAAAAAGCTCTTCTGCTGCGCGAGTGAAACTTAAATGGCGCGCTGCGGCATCAAAAACTCGTAAGGCAt                                                               >  2:1217945/1‑93 (MQ=255)

                         gatgaCTTACTGCGGCTTGGGTCACAAAAAGCTCTTCTGCTGCGCGAGTGAAACTTAAATGGCGCGCTGCGGCATCAAAAACTCGTAAGGCAt                                                               >  1:3793978/1‑93 (MQ=255)

                          atgaCTTACTGCGGCTTGGGTCACAAAAAGCTCTTCTGCTGCGCGAGTGAAACTTAAATGGCGCGCTGCGGCATCAAAAACTCGTAAGGCAtt                                                              <  2:1889694/93‑1 (MQ=255)

                           tgaCTTACTGCGGCTTGGGTCACAAAAAGCTCTTCTGCTGCGCGAGTGAAACTTAAATGGCGCGCTGCGGCATCAAAAACTCGTAAGGCAttt                                                             >  2:555938/1‑93 (MQ=255)

                           tgaCTTACTGCGGCTTGGGTCACAAAAAGCTCTTCTGCTGCGCGAGTGAAACTTAAATGGCGCGCTGCGGCATCAAAAACTCGTAAGGCAttt                                                             >  1:4337864/1‑93 (MQ=255)

                            gaCTTACTGCGGCTTGGGTCACAAAAAGCTCTTCTGCTGCGCGAGTGAAACTTAAATGGCGCGCTGCGGCATCAAAAACTCGTAAGGCATTTa                                                            >  2:3817979/1‑93 (MQ=255)

                            gaCTTACTGCGGCTTGGGTCACAAAAAGCTCTTCTGCTGCGCGAGTGAAACTTAAATGGCGCGCTGCGGCATCAAAAACTCGTAAGGCATTTa                                                            <  2:3900024/93‑1 (MQ=255)

                             aCTTACTGCGGCTTGGGTCACAAAAAGCTCTTCTGCTGCGCGAGTGAAACTTAAATGGCGCGCTGCGGCATCAAAAACTCGTAAGGCATTTAg                                                           >  2:2059632/1‑93 (MQ=255)

                             aCTTACTGCGGCTTGGGTCACAAAAAGCTCTTCTGCTGCGCGAGTGAAACTTAAATGGCGCGCTGCGGCATCAAAAACTCGTAAGGCATTTAg                                                           >  2:723656/1‑93 (MQ=255)

                             aCTTACTGCGGCTTGGGTCACAAAAAGCTCTTCTGCTGCGCGAGTGAAACTTAAATGGCGCGCTGCGGCATCAAAAACTCGTAAGGCATTTAg                                                           <  2:4414115/93‑1 (MQ=255)

                              cTTACTGCGGCTTGGGTCACAAAAAGCTCTTCTGCTGCGCGAGTGAAACTTAAATGGCGCGCTGCGGCATCAAAAACTCGTAAGGCATTTAGc                                                          <  2:2859240/93‑1 (MQ=255)

                              cTTACTGCGGCTTGGGTCACAAAAAGCTCTTCTGCTGCGCGAGTGAAACTCAAATGGCGCGCTGCGGCATCAAAAACTCGTAAGGCATTTAGc                                                          <  1:2516397/93‑1 (MQ=255)

                                 aCTGCGGCTTGGGTCACAAAAAGCTCTTCTGCTGCGCGAGTGAAACTTAAATGGCGCGCTGCGGCATCAAAAACTCGTAAGGCATTTAGCggt                                                       >  2:2753066/1‑93 (MQ=255)

                                 aCTGCGGCTTGGGTCACAAAAAGCTCTTCTGCTGCGCGAGTGAAACTTAAATGGCGCGCTGCGGCATCAAAAACTCGTAAGGCATTTAGCggt                                                       <  2:1082642/93‑1 (MQ=255)

                                 aCTGCGGCTTGGGTCACAAAAAGCTCTTCTGCTGCGCGAGTGAAACTTAAATGGCGCGCTGCGGCATCAAAAACTCGTAAGGCATTTAGCggt                                                       <  2:671084/93‑1 (MQ=255)

                                  cTGCGGCTTGGGTCACAAAAAGCTCTTCTGCTGCGCGAGTGAAACTTAAATGGCGCGCTGCGGCATCAAAAACTCGTAAGGCATTTAGCggtg                                                      >  2:2048049/1‑93 (MQ=255)

                                   tGCGGCTTGGGTCACAAAAAGCTCTTCTGCTGCGCGAGTGAAACTTAAATGGCGCGCTGCGGCATCAAAAACTCGTAAGGCATTTAGCggtgg                                                     >  1:4540177/1‑93 (MQ=255)

                                   tGCGGCTTGGGTCACAAAAAGCTCTTCTGCTGCGCGAGTGAAACTTAAATGGCGCGCTGCGGCATCAAAAACTCGTAAGGCATTTAGCggtgg                                                     <  2:2621913/93‑1 (MQ=255)

                                   tGCGGCTTGGGTCACAAAAAGCTCTTCTGCTGCGCGAGTGAAACTTAAATGGCGCGCTGCGGCATCAAAAACTCGTAAGGCATTTAGCggtgg                                                     >  1:2797144/1‑93 (MQ=255)

                                    gCGGCTTGGGTCACAAAAAGCTCTTCTGCTGCGCGAGTGAAACTTAAATGGCGCGCTGCGGCATCAAAAACTCGTAAGGCATTTAGCggtggt                                                    <  2:3866203/93‑1 (MQ=255)

                                      ggCTTGGGTCACAAAAAGCTCTTCTGCTGCGCGAGTGAAACTTAAATGGCGCGCTGCGGCATCAAAAACTCGTAAGGCATTTAGCGGTGGTaa                                                  <  2:1628804/93‑1 (MQ=255)

                                       gCTTGGGTCACAAAAAGCTCTTCTGCTGCGCGAGTGAAACTTAAATGGCGCGCTGCGGCATCAAAAACTCGTAAGGCATTTAGCGGTGGTAAt                                                 <  2:542897/93‑1 (MQ=255)

                                       gCTTGGGTCACAAAAAGCTCTTCTGCTGCGCGAGTGAAACTTAAATGGCGCGCTGCGGCATCAAAAACTCGTAAGGCATTTAGCGGTGGTAAt                                                 <  2:772900/93‑1 (MQ=255)

                                         ttGGGTCACAAAAAGCTCTTCTGCTGCGCGAGTGAAACTTAAATGGCGCGCTGCGGCATCAAAAACTCGTAAGGCATTTAGCGGTGGTAATCg                                               >  2:1993339/1‑93 (MQ=255)

                                         ttGGGTCACAAAAAGCTCTTCTGCTGCGCGAGTGAAACTTAAATGGCGCGCTGCGGCATCAAAAACTCGTAAGGCATTTAGCGGTGGTAATCg                                               >  2:3023174/1‑93 (MQ=255)

                                         ttGGGTCACAAAAAGCTCTTCTGCTGCGCGAGTGAAACTTAAATGGCGCGCTGCGGCATCAAAAACTCGTAAGGCATTTAGCGGTGGTAATCg                                               >  2:4038429/1‑93 (MQ=255)

                                            ggTCACAAAAAGCTCTTCTGCTGCGCGAGTGAAACTTAAATGGCGCGCTGCGGCATCAAAAACTCGTAAGGCATTTAGCGGTGGTAATCGttt                                            <  1:580038/93‑1 (MQ=255)

                                            ggTCACAAAAAGCTCTTCTGCTGCGCGAGTGAAACTTAAATGGCGCGCTGCGGCATCAAAAACTCGTAAGGCATTTAGCGGTGGTAATCGttt                                            >  2:4516780/1‑93 (MQ=255)

                                             gTCACAAAAAGCTCTTCTGCTGCGCGAGTGAAACTTAAATGGCGCGCTGCGGCATCAAAAACTCGTAAGGCATTTAGCGGTGGTAATCGTTTa                                           >  2:3110545/1‑93 (MQ=255)

                                             gTCACAAAAAGCTCTTCTGCTGCGCGAGGGAAACTTAAATGGCGCGCTGCGGCATCAAAAACTCGTAAGGCATTTAGCGGTGGTAATCGTTTa                                           >  2:3420050/1‑93 (MQ=255)

                                               cacaAAAAGCTCTTCTGCTGCGCGAGTGAAACTTAAATGGCGCGCTGCGGCATCAAAAACTCGTAAGGCATTTAGCGGTGGTAATCGTTTAGa                                         <  2:2939859/93‑1 (MQ=255)

                                               cacaAAAAGCTCTTCTGCTGCGCGAGTGAAACTTAAATGGCGCGCTGCGGCATCAAAAACTCGTAAGGCATTTAGCGGTGGTAATCGTTTAGa                                         <  2:896883/93‑1 (MQ=255)

                                                acaAAAAGCTCTTCTGCTGCGCGAGTGAAACTTAAATGGCGCGCTGCGGCATCAAAAACTCGTAAGGCATTTAGCGGTGGTAATCGTTTAGAc                                        >  2:733610/1‑93 (MQ=255)

                                                 caAAAAGCTCTTCTGCTGCGCGAGTGAAACTTAAATGGCGCGCTGCGGCATCAAAAACTCGTAAGGCATTTAGCGGTGGTAATCGTTTAGACa                                       >  1:1013731/1‑93 (MQ=255)

                                                  aaaaaGCTCTTCTGCTGCGCGAGTGAAACTTAAATGGCGCGCTGCGGCATCAAAAACTCGTAAGGCATTTAGCGGTGGTAATCGTTTAGACAt                                      <  1:2155769/93‑1 (MQ=255)

                                                   aaaaGCTCTTCTGCTGCGCGAGTGAAACTTAAATGGCGCGCTGCGGCATCAAAAACTCGTAAGGCATTTAGCGGTGGTAATCGTTTAGACATg                                     <  2:3484427/93‑1 (MQ=255)

                                                    aaaGCTCTTCTGCTGCGCGAGTGAAACTTAAATGGCGCGCTGCGGCATCAAAAACTCGTAAGGCATTTAGCGGTGGTAATCGTTTAGACATgg                                    <  1:3396915/93‑1 (MQ=255)

                                                     aaGCTCTTCTGCTGCGCGAGTGAAACTTAAATGGCGCGCTGCGGCATCAAAAACTCGTAAGGCATTTAGCGGTGGTAATCGTTTAGACATGGc                                   >  1:2728493/1‑93 (MQ=255)

                                                      aGCTCTTCTGCTGCGCGAGTGAAACTTAAATGGCGCGCTGCGGCATCAAAAACTCGTAAGGCATTTAGCGGTGGTAATCGTTTAGACATGGCt                                  >  1:4573303/1‑93 (MQ=255)

                                                       gCTCTTCTGCTGCGCGAGTGAAACTTAAATGGCGCGCTGCGGCATCAAAAACTCGTAAGGCATTTAGCGGTGGTAATCGTTTAGACATGGCTa                                 <  1:3873620/93‑1 (MQ=255)

                                                        ctctTCTGCTGCGCGAGTGAAACTTAAATGGCGCGCTGCGGCATCAAAAACTCGTAAGGCATTTAGCGGTGGTAATCGTTTAGACATGGCTAt                                <  1:605087/93‑1 (MQ=255)

                                                        ctctTCTGCTGCGCGAGTGAAACTTAAATGGCGCGCTGCGGCATCAAAAACTCGTAAGGCATTTAGCGGTGGTAATCGTTTAGACATGGCTAt                                <  2:4328119/93‑1 (MQ=255)

                                                         tcttctGCTGCGCGAGTGAAACTTAAATGGCGCGCTGCGGCATCAAAAACTCGTAAGGCATTTAGCGGTGGTAATCGTTTAGACATGGCTAtt                               >  2:124295/1‑93 (MQ=255)

                                                         tcttctGCTGCGCGAGTGAAACTTAAATGGCGCGCTGCGGCATCAAAAACTCGTAAGGCATTTAGCGGTGGTAATCGTTTAGACATGGCTAtt                               >  2:3143785/1‑93 (MQ=255)

                                                          cttctGCTGCGCGAGTGAAACTTAAATGGCGCGCTGCGGCATCAAAAACTCGTAAGGCATTTAGCGGTGGTAATCGTTTAGACATGGCTATTa                              >  1:3494373/1‑93 (MQ=255)

                                                           ttctGCTGCGCGAGTGAAACTTAAATGGCGCGCTGCGGCATCAAAAACTCGTAAGGCATTTAGCGGTGGTAATCGTTTAGACATGGCTATTaa                             <  2:2368586/93‑1 (MQ=255)

                                                            tctGCTGCGCGAGTGAAACTTAAATGGCGCGCTGCGGCATCAAAAACTCGTAAGGCATTTAGCGGTGGTAATCGTTTAGACATGGCTATTaaa                            >  2:3944571/1‑93 (MQ=255)

                                                             ctgctgCGCGAGTGAAACTTAAATGGCGCGCTGCGGCATCAAAAACTCGTAAGGCATTTAGCGGTGGTAATCGTTTAGACATGGCTATTAAAc                           <  2:127433/93‑1 (MQ=255)

                                                             ctgctgCGCGAGTGAAACTTAAATGGCGCGCTGCGGCATCAAAAACTCGTAAGGCATTTAGCGGTGGTAATCGTTTAGACATGGCTATTAAAc                           >  1:3034656/1‑93 (MQ=255)

                                                              tgctgcGCGAGTGAAACTTAAATGGCGCGCTGCGGCATCAAAAACTCGTAAGGCATTTAGCGGTGGTAATCGTTTAGACATGGCTATTAAACt                          >  2:3633516/1‑93 (MQ=255)

                                                               gctgcGCGAGTGAAACTTAAATGGCGCGCTGCGGCATCAAAAACTCGTAAGGCATTTAGCGGTGGTAATCGTTTAGACATGGCTATTAAACtt                         <  1:1129413/93‑1 (MQ=255)

                                                                 tgcGCGAGTGAAACTTAAATGGCGCGCTGCGGCATCAAAAACTCGTAAGGCATTTAGCGGTGGTAATCGTTTAGACATGGCTATTAAACTTTg                       >  2:2465893/1‑93 (MQ=255)

                                                                  gcgcGAGTGAAACTTAAATGGCGCGCTGCGGCATCAAAAACTCGTAAGGCATTTAGCGGTGGTAATCGTTTAGACATGGCTATTAAACTTTGa                      <  2:3327127/93‑1 (MQ=255)

                                                                  gcgcGAGTGAAACTTAAATGGCGCGCTGCGACATCAAAAACTCGTAAGGCATTTAGCGGTGGTAATCGTTTAGACATGGCTATTAAACTTTGa                      <  2:3438802/93‑1 (MQ=255)

                                                                        gTGAAACTTAAATGGCGCGCTGCGGCATCAAAAACTCGTAAGGCATTTAGCGGTGGTAATCGTTTAGACATGGCTATTAAACTTTGATGTTaa                <  1:356056/93‑1 (MQ=255)

                                                                         tGAAACTTAAATGGCGCGCTGCGGCATCAAAAACTCGTAAGGCATTTAGCGGTGGTAATCGTTTAGACATGGCTATTAAACTTTGATGTTaaa               >  1:48606/1‑93 (MQ=255)

                                                                            aaCTTAAATGGCGCGCTGCGGCATCAAAAACTCGTAAGGCATTTAGCGGTGGTAATCGTTTAGACATGGCTATTAAACTTTGATGTTAAATGa            >  2:218066/1‑93 (MQ=255)

                                                                            aaCTTAAATGGCGCGCTGCGGCATCAAAAACTCGTAAGGCATTTAGCGGTGGTAATCGTTTAGACATGGCTATTAAACTTTGATGTTAAATGa            >  1:4617978/1‑93 (MQ=255)

                                                                            aaCTTAAATGGCGCGCTGCGGCATCAAAAACTCGTAAGGCATTTAGCGGTGGTAATCGTTTAGACATGGCTATTAAACTTTGATGTTAAATGa            <  1:3205711/93‑1 (MQ=255)

                                                                            aaCTTAAATGGCGCGCTGCGGCATCAAAAACTCGTAAGGCATTTAGCGGTGGTAATCGTTTAGACATGGCTATTAAACTTTGATGTTAAATGa            >  1:1615661/1‑93 (MQ=255)

                                                                            aaCTTAAATGGCGCGCTGCGGCATCAAAAACTCGTAAGGCATTTAGCGGTGGTAATCGTTTAGACATGGCTATTAAACTTTGATGTTAAATGa            >  1:2579257/1‑93 (MQ=255)

                                                                             aCTTAAATGGCGCGCTGCGGCATCAAAAACTCGTAAGGCATTTAGCGGTGGTAATCGTTTAGACATGGCTATTAAACTTTGATGTTAAATGaa           <  2:3260167/93‑1 (MQ=255)

                                                                             aCTTAAATGGCGCGCTGCGGCATCAAAAACTCGTAAGGCATTTAGCGGTGGTAATCGTTTAGACATGGCTATTAAACTTTGATGTTAAATGaa           <  1:1277397/93‑1 (MQ=255)

                                                                             aCTTAAATGGCGCGCTGCGGCATCAAAAACTCGTAAGGCATTTAGCGGTGGTAATCGTTTAGACATGGCTATTAAACTTTGATGTTAAATGaa           >  2:1403453/1‑93 (MQ=255)

                                                                                  aaTGGCGCGCTGCGGCATCAAAAACTCGTAAGGCATTTAGCGGTGGTAATCGTTTAGACATGGCTATTAAACTTTGATGTTAAATGAATTTaa      >  2:1967260/1‑93 (MQ=255)

                                                                                   aTGGCGCGCTGCGGCATCAAAAACTCGTAAGGCATTTAGCGGTGGTAATCGTTTAGACATGGCTATTAAACTTTGATGTTAAATGAATTTAAc     <  2:1447433/93‑1 (MQ=255)

                                                                                    tGGCGCGCTGCGGCATCAAAAACTCGTAAGGCATTTAGCGGTGGTAATCGTTTAGACATGGCTATTAAACTTTGATGTTAAATGAATTTAACa    >  2:1850651/1‑93 (MQ=255)

                                                                                     ggCGCGCTGCGGCATCAAAAACTCGTAAGGCATTTAGCGGTGGTAATCGTTTAGACATGGCTATTAAACTTTGATGTTAAATGAATTTAACaa   <  2:284597/93‑1 (MQ=255)

                                                                                     ggCGCGCTGCGGCATCAAAAACTCGTAAGGCATTTAGCGGTGGTAATCGTTTAGACATGGCTATTAAACTTTGATGTTAAATGAATTTAACaa   <  1:4641702/93‑1 (MQ=255)

                                                                                     ggCGCGCTGCGGCATCAAAAACTCGTAAGGCATTTAGCGGTGGTAATCGTTTAGACATGGCTATTAAACTTTGATGTTAAATGAATTTAACaa   <  2:1533213/93‑1 (MQ=255)

                                                                                     ggCGCGCTGCGGCATCAAAAACTCGTAAGGCATTTAGCGGTAGTAATCGTTTAGACATGGCTATTAAACTTTGATGTTAAATGAATTTAACaa   <  1:1441126/93‑1 (MQ=255)

                                                                                      gcgcgcTGCGGCATCAAAAACTCGTAAGGCATTTAGCGGTGGTAATCGTTTAGACATGGCTATTAAACTTTGATGTTAAATGAATTTAACAAt  <  2:3537453/93‑1 (MQ=255)

AAAAAATCCTCAAGAGACTTGATTTGATGACTTACTGCGGCTTGGGTCACAAAAAGCTCTTCTGCTGCGCGAGTGAAACTTAAATGGCGTGCTGCGGCATCAAAAACTCGTAAGGCATTTAGCGGTGGTAATCGTTTAGACATGGCTATTAAACTTTGATGTTAAATGAATTTAACAAT  >  NC_000913/2940447‑2940625

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 23 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

CCCCAAAAAATCCTCAAGAGACTTGATTTGATGACTTACTGCGGCTTGGGTCACAAAAAGCTCTTCTGCTGCGCGAGTGAAACTTAAATGGCGTGCTGCGGCATCAAAAACTCGTAAGGCATTTAGCGGTGGTAATCGTTTAGACATGGCTATTAAACTTTGATGTTAAATGAATTTAACAATTAGA  >  NC_000913/2940443‑2940629

CCCCAAAAAATCCTCAAGAGACTTGATTTGATGACTTACTGCGGCTTGGGTCACAAAAAGCTCTTCTGCTGCGCGAGTGAAACTTAAATGGCGC                                                                                               >  SRR4302535.2790854/1‑94 (MQ=60)

 CCCAAAAAATCCTCAAGAGACTTGATTTGATGACTTACTGCGGCTTGGGTCACAAAAAGCTCTTCTGCTGCGCGAGTGAAACTTAAATGGCGCG                                                                                              >  SRR4302535.1178706/1‑94 (MQ=60)

 CCCAAAAAATCCTCAAGAGACTTGATTTGATGACTTACTGCGGCTTGGGTCACAAAAAGCTCTTCTGCTGCGCGAGTGAAACTTAAATGGCGCG                                                                                              >  SRR4302535.3191002/1‑94 (MQ=60)

   CAAAAAATCCTCAAGAGACTTGATTTGATGACTTACTGCGGCTTGGGTCACAAAAAGCTCTTCTGCTGCGCGAGTGAAACTTAAATGGCGCGCT                                                                                            >  SRR4302535.4157632/1‑94 (MQ=60)

     AAAAATCCTCAAGAGACTTGATTTGATGACTTACTGCGGCTTGGGTCACAAAAAGCTCTTCTGCTGCGCGAGTGAAACTTAAATGGCGCGCTGC                                                                                          >  SRR4302535.1975182/1‑94 (MQ=60)

      AAAATCCTCAAGAGACTTGATTTGATGACTTACTGCGGCTTGGGTCACAAAAAGCTCTTCTGCTGCGCGAGTGAAACTTAAATGGCGCGCTGCG                                                                                         <  SRR4302535.3726475/94‑1 (MQ=60)

      AAAATCCTCAAGAGACTTGATTTGATGACTTACTGCGGCTTGGGTCACAAAAAGCTCTTCTGCTGCGCGAGTGAAACTTAAATGGCGCGCTGCG                                                                                         >  SRR4302535.3110582/1‑94 (MQ=60)

       AAATCCTCAAGAGACTTGATTTGATGACTTACTGCGGCTTGGGTCACAAAAAGCTCTTCTGCTGCGCGAGTGAAACTTAAATGGCGCGCTGCGG                                                                                        >  SRR4302535.716775/1‑94 (MQ=60)

        AATCCTCAAGAGACTTGATTTGATGACTTACTGCGGCTTGGGTCACAAAAAGCTCTTCTGCTGCGCGAGTGAAACTTAAATGGCGCGCTGCGGC                                                                                       >  SRR4302535.148893/1‑94 (MQ=60)

          TCCTCAAGAGACTTGATTTGATGACTTACTGCGGCTTGGGTCACAAAAAGCTCTTCTGCTGCGCGAGTGAAACTTAAATGGCGCGCTGCGGCAT                                                                                     >  SRR4302535.4602573/1‑94 (MQ=60)

          TCCTCAAGAGACTTGATTTGATGACTTACTGCGGCTTGGGTCACAAAAAGCTCTTCTGCTGCGCGAGTGAAACTTAAATGGCGCGCTGCGGCAT                                                                                     <  SRR4302535.4236531/94‑1 (MQ=60)

             TCAAGAGACTTGATTTGATGACTTACTGCGGCTTGGGTCACAAAAAGCTCTTCTGCTGCGCGAGTGAAACTTAAATGGCGCGCTGCGGCATCTA                                                                                  >  SRR4302535.1526958/1‑94 (MQ=60)

             TCAAGAGACTTGATTTGATGACTTACTGCGGCTTGGGTCACAAAAAGCTCTTCTGCTGCGCGAGTGAAACTTAAATGGCGCGCTGCGGCATCAA                                                                                  <  SRR4302535.999990/94‑1 (MQ=60)

             TCAAGAGACTTGATTTGATGACTTACTGCGGCTTGGGTCACAAAAAGCTCTTCTGCTGCGCGAGTGAAACTTAAATGGCGCGCTGCGGCATCAA                                                                                  >  SRR4302535.349902/1‑94 (MQ=60)

                 GAGACTTGATTTGATGACTTACTGCGGCTTGGGTCACAAAAAGCTCTTCTGCTGCGCGAGTGAAACTTAAATGGCGCGCTGCGGCATCAAAAAC                                                                              <  SRR4302535.1040085/94‑1 (MQ=60)

                  AGACTTGATTTGATGACTTACTGCGGCTTGGGTCACAAAAAGCTCTTCTGCTGCGCGAGTGAAACTTAAATGGCGCGCTTCGGCATCAAAAACT                                                                             >  SRR4302535.2121626/1‑94 (MQ=60)

                  AGACTTGATTTGATGACTTACTGCGGCTTGGGTCACAAAAAGCTCTTCTGCTGCGCGAGTGAAACTTAAATGGCGCGCTGCGGCATCAAAAACT                                                                             <  SRR4302535.2783369/94‑1 (MQ=60)

                   GACTTGATTTGATGACTTACTGCGGCTTGGGTCACAAAAAGCTCTTCTGCTGCGCGAGTGAAACTTAAATGGCGCGCTGCGGCATCAAAAACTC                                                                            >  SRR4302535.4634060/1‑94 (MQ=60)

                    ACTTGATTTGATGACTTACTGCGGCTTGGGTCACAAAAAGCTCTTCTGCTGCGCGAGTGAAACTTAAATGGCGCGCTGCGGCATCAAAAACTCG                                                                           >  SRR4302535.3534940/1‑94 (MQ=60)

                     CTTGATTTGATGACTTACTGCGGCTTGGGTCACAAAAAGCTCTTCTGCTGCGCGAGTGAAACTTAAATGGCGCGCTGCGGCATCAAAAACTCGT                                                                          >  SRR4302535.3428078/1‑94 (MQ=60)

                       TGATTTGATGACTTACTGCGGCTTGGGTCACAAAAAGCTCTTCTGCTGCGCGAGTGAAACTTAAATGGCGCGCTGCGGCATCAAAAACTCGTAA                                                                        >  SRR4302535.1269229/1‑94 (MQ=60)

                        GATTTGATGACTTACTGCGGCTTGGGTCACAAAAAGCTCTTCTGCTGCGCGAGTGAAACTTAAATGGCGCGCTGCGGCATCAAAAACTCGTAAG                                                                       <  SRR4302535.1491/94‑1 (MQ=60)

                          TTTGATGACTTACTGCGGCTTGGGTCACAAAAAGCTCTTCTGCTGCGCGAGTGAAACTTAAATGGCGCGCTGCGGCATCAAAAACTCGTAAGGC                                                                     >  SRR4302535.4364176/1‑94 (MQ=60)

                          TTTGATGACTTACTGCGGCTTGGGTCACAAAAAGCTCTTCTGCTGCGCGAGTGAAACTTAAATGGCGCGCTGCGGCATCAAAAACTCGTAAGGC                                                                     <  SRR4302535.274036/94‑1 (MQ=60)

                           TTGATGACTTACTGCGGCTTGGGTCACAAAAAGCTCTTCTGCTGCGCGAGTGAAACTTAAATGGCGCGCTGCGGCATCAAAAACTCGTAAGGCA                                                                    <  SRR4302535.2566021/94‑1 (MQ=60)

                            TGATGACTTACTGCGGCTTGGGTCACAAAAAGCTCTTCTGCTGCGCGAGTGAAACTTAAATGGCGCGCTGCGGCATCAAAAACTCGTAAGGCAT                                                                   >  SRR4302535.3820134/1‑94 (MQ=60)

                            TGATGACTTACTGCGGCTTGGGTCACAAAAAGCTCTTCTGCTGCGCGAGTGAAACTTAAATGGCGCGCTGCGGCATCAAAAACTCGTAAGGCAT                                                                   >  SRR4302535.3868356/1‑94 (MQ=60)

                              ATGACTTACTGCGGCTTGGGTCACAAAAAGCTCTTCTGCTGCGCGAGTGAAACTTAAATGGCGCGCTGCGGCATCAAAAACTCGTAAGGCATTT                                                                 >  SRR4302535.4367530/1‑94 (MQ=60)

                               TGACTTACTGCGGCTTGGGTCACAAAAAGCTCTTCTGCTGCGCGAGTGAAACTTAAATGGCGCGCTGCGGCATCAAAAACTCGTAAGGCATTTA                                                                <  SRR4302535.2205292/94‑1 (MQ=60)

                                GACTTACTGCGGCTTGGGTCACAAAAAGCTCTTCTGCTGCGCGAGTGAAACTTAAATGGCGCGCTGCGGCATCAAAAACTCGTAAGGCATTTAG                                                               <  SRR4302535.1271586/94‑1 (MQ=60)

                                  CTTACTGCGGCTTGGGTCACAAAAAGCTCTTCTGCTGCGCGAGTGAAACTTAAATGGCGCGCTGCGGCATCAAAAACTCGTAAGGCATTTAGCG                                                             <  SRR4302535.2572751/94‑1 (MQ=60)

                                  CTTACTGCGGCTTGGGTCACAAAAAGCTCTTCTGCTGCGCGAGTGAAACTTAAATGGCGCGCTGCGGCATCAAAAACTCGTAAGGCATTTAGCG                                                             >  SRR4302535.948069/1‑94 (MQ=60)

                                  CTTACTGCGGCTTGGGTCACAAAAAGCTCTTCTGCTGCGCGAGTGAAACTCAAATGGCGCGCTGCGGCATCAAAAACTCGTAAGGCATTTAGCG                                                             <  SRR4302535.2534535/94‑1 (MQ=60)

                                     ACTGCGGCTTGGGTCACAAAAAGCTCTTCTGCTGCGCGAGTGAAACTTAAATGGCGCGCTGCGGCATCAAAAACTCGTAAGGCATTTAGCGGTG                                                          <  SRR4302535.469575/94‑1 (MQ=60)

                                      CTGCGGCTTGGGTCACAAAAAGCTCTTCTGCTGCGCGAGTGAAACTTAAATGGCGCGCTGCGGCATCAAAAACTCGTAAGGCATTTAGCGGTGG                                                         >  SRR4302535.2817048/1‑94 (MQ=60)

                                      CTGCGGCTTGGGTCACAAAAAGCTCTTCTGCTGCGCGAGTGAAACTTAAATGGCGCGCTGCGGCATCAAAAACTCGTAAGGCATTTAGCGGTGG                                                         >  SRR4302535.4571230/1‑94 (MQ=60)

                                         CGGCTTGGGTCACAAAAAGCTCTTCTGCTGCGCGAGTGAAACTTAAATGGCGCGCTGCGGCATCAAAAACTCGTAAGGCATTTAGCGGTGGTAA                                                      >  SRR4302535.4658273/1‑94 (MQ=60)

                                          GGCTTGGGTCACAAAAAGCTCTTCTGCTGCGCGAGTGAAACTTAAATGGCGCGCTGCGGCATCAAAAACTCGTAAGGCATTTAGCGGTGGTAAT                                                     <  SRR4302535.4545622/94‑1 (MQ=60)

                                          GGCTTGGGTCACAAAAAGCTCTTCTGCTGCGCGAGTGAAACTTAAATGGCGCGCTGCGGCATCAAAAACTCGTAAGGCATTTAGCGGTGGTAAT                                                     <  SRR4302535.4162500/94‑1 (MQ=60)

                                            CTTGGGTCACAAAAAGCTCTTCTGCTGCGCGAGTGAAACTTAAATGGCGCGCTGCGGCATCAAAAACTCGTAAGGCATTTAGCGGTGGTAATCG                                                   >  SRR4302535.1124673/1‑94 (MQ=60)

                                               GGGTCACAAAAAGCTCTTCTGCTGCGCGAGTGAAACTTAAATGGCGCGCTGCGGCATCAAAAACTCGTAAGGCATTTAGCGGTGGTAATCGTTT                                                <  SRR4302535.2687501/94‑1 (MQ=60)

                                                GGTCACAAAAAGCTCTTCTGCTGCGCGAGTGAAACTTAAATGGCGCGCTGCGGCATCAAAAACTCGTAAGGCATTTAGCGGTGGTAATCGTTTA                                               <  SRR4302535.584117/94‑1 (MQ=60)

                                                   CACAAAAAGCTCTTCTGCTGCGCGAGTGAAACTTAAATGGCGCGCTGCGGCATCAAAAACTCGTAAGGCATTTAGCGGTGGTAATCGTTTAGAC                                            <  SRR4302535.1367964/94‑1 (MQ=60)

                                                    ACAAAAAGCTCTTCTGCTGCGCGAGTGAAACTTAAATGGCGCGCTGCGGCATCAAAAACTCGTAAGGCATTTAGCGGTGGTAATCGTTTAGACA                                           >  SRR4302535.1020939/1‑94 (MQ=60)

                                                     CAAAAAGCTCTTCTGCTGCGCGAGTGAAACTTAAATGGCGCGCTGCGGCATCAAAAACTCGTAAGGCATTTAGCGGTGGTAATCGTTTAGACAT                                          >  SRR4302535.3416916/1‑94 (MQ=60)

                                                      AAAAAGCTCTTCTGCTGCGCGAGTGAAACTTAAATGGCGCGCTGCGGCATCAAAAACTCGTAAGGCATTTAGCGGTGGTAATCGTTTAGACATG                                         <  SRR4302535.2171260/94‑1 (MQ=60)

                                                        AAAGCTCTTCTGCTGCGCGAGTGAAACTTAAATGGCGCGCTGCGGCATCAAAAACTCGTAAGGCATTTAGCGGTGGTAATCGTTTAGACATGGC                                       <  SRR4302535.3420549/94‑1 (MQ=60)

                                                        AAAGCTCTTCTGCTGCGCGAGTGAAACTTAAATGGCGCGCTGCGGCATCAAAAACTCGTAAGGCATTTAGCGGTGGTAATCGTTTAGACATGGC                                       >  SRR4302535.2747976/1‑94 (MQ=60)

                                                         AAGCTCTTCTGCTGCGCGAGTGAAACTTAAATGGCGCGCTGCGGCATCAAAAACTCGTAAGGCATTTAGCGGTGGTAATCGTTTAGACATGGCT                                      >  SRR4302535.4604478/1‑94 (MQ=60)

                                                         AAGCTCTTCTGCTGCGCGAGTGAAACTTAAATGGCGCGCTGCGGCATCAAAAACTCGTAAGGCATTTAGCGGTGGTAATCGTTTAGACATGGCT                                      >  SRR4302535.4284102/1‑94 (MQ=60)

                                                         AAGCTCTTCTGCTGCGCGAGTGAAACTTAAATGGCGCGCTGCGGCATCAAAAACTCGTAAGGCATTTAGCGGTGGTAATCGTTTAGACATGGCT                                      <  SRR4302535.4089705/94‑1 (MQ=60)

                                                          AGCTCTTCTGCTGCGCGAGTGAAACTTAAATGGCGCGCTGCGGCATCAAAAACTCGTAAGGCATTTAGCGGTGGTAATCGTTTAGACATGGCTA                                     >  SRR4302535.2020258/1‑94 (MQ=60)

                                                           GCTCTTCTGCTGCGCGAGTGAAACTTAAATGGCGCGCTGCGGCATCAAAAACTCGTAAGGCATTTAGCGGTGGTAATCGTTTAGACATGGCTAT                                    <  SRR4302535.3900238/94‑1 (MQ=60)

                                                            CTCTTCTGCTGCGCGAGTGAAACTTAAATGGCGCGCTGCGGCATCAAAAACTCGTAAGGCATTTAGCGGTGGTAATCGTTTAGACATGGCTATT                                   <  SRR4302535.3437417/94‑1 (MQ=60)

                                                            CTCTTCTGCTGCGCGAGTGAAACTTAAATGGCGCGCTGCGGCATCAAAAACTCGTAAGGCATTTAGCGGTGGTAATCGTTTAGACATGGCTATT                                   <  SRR4302535.609319/94‑1 (MQ=60)

                                                             TCTTCTGCTGCGCGAGTGAAACTTAAATGGCGCGCTGCGGCATCAAAAACTCGTAAGGCATTTAGCGGTGGTAATCGTTTAGACATGGCTATTA                                  >  SRR4302535.3518652/1‑94 (MQ=60)

                                                              CTTCTGCTGCGCGAGTGAAACTTAAATGGCGCGCTGCGGCATCAAAAACTCGTAAGGCATTTAGCGGTGGTAATCGTTTAGACATGGCTATTAA                                 >  SRR4302535.1943108/1‑94 (MQ=60)

                                                                TCTGCTGCGCGAGTGAAACTTAAATGGCGCGCTGCGGCATCAAAAACTCGTAAGGCATTTAGCGGTGGTAATCGTTTAGACATGGCTATTAAAC                               <  SRR4302535.3641071/94‑1 (MQ=60)

                                                                TCTGCTGCGCGAGTGAAACTTAAATGGCGCGCTGCGGCATCAAAAACTCGTAAGGCATTTAGCGGTGGTAATCGTTTAGACATGGCTATTAAAC                               >  SRR4302535.3055900/1‑94 (MQ=60)

                                                                 CTGCTGCGCGAGTGAAACTTAAATGGCGCGCTGCGGCATCAAAAACTCGTAAGGCATTTAGCGGTGGTAATCGTTTAGACATGGCTATTAAACT                              >  SRR4302535.3083680/1‑94 (MQ=60)

                                                                   ggcGCGCGAGTGAAACTTAAATGGCGCGCTGCGACATAAAAAACTCGTAAGGTATTTAGCGGTGGTAATCGTTTAGACATGGCTATTAAACTTT                            <  SRR4302535.3523169/91‑1 (MQ=60)

                                                                   GCTGCGCGAGTGAAACTTAAATGGCGCGCTGCGGCATCAAAAACTCGTAAGGCATTTAGCGGTGGTAATCGTTTAGACATGGCTATTAAACTTT                            <  SRR4302535.1137475/94‑1 (MQ=60)

                                                                    CTGCGCGAGTGAAACTTAAATGGCGCGCTGCGGCATCAAAAACTCGTAAGGCATTTAGCGGTGGTAATCGTTTAGACATGGCTATTAAACTTTG                           <  SRR4302535.1505340/94‑1 (MQ=60)

                                                                    CTGCGCGAGTGAAACTTAAATGGCGCGCTGCGGCATCAAAAACTCGTAAGGCATTTAGCGGTGGTAATCGTTTAGACATGGCTATTAAACTTTG                           <  SRR4302535.2740961/94‑1 (MQ=60)

                                                                       CGCGAGTGAAACTTAAATGGCGCGCTGCGGCATCAAAAACTCGTAAGGCATTTAGCGGTGGTAATCGTTTAGACATGGCTATTAAACTTTGATG                        >  SRR4302535.3505788/1‑94 (MQ=60)

                                                                        GCGAGTGAAACTTAAATGGCGCGCTGCGGCATCAAAAACTCGTAAGGCATTTAGCGGTGGTAATCGTTTAGACATGGCTATTAAACTTTGATGT                       <  SRR4302535.2159896/94‑1 (MQ=60)

                                                                            GTGAAACTTAAATGGCGCGCTGCGGCATCAAAAACTCGTAAGGCATTTAGCGGTGGTAATCGTTTAGACATGGCTATTAAACTTTGATGTTAAA                   >  SRR4302535.48974/1‑94 (MQ=60)

                                                                            GTGAAACTTAAATGGCGCGCTGCGGCATCAAAAACTCGTAAGGCATTTAGCGGTGGTAATCGTTTAGACATGGCTATTAAACTTTGATGTTAAA                   <  SRR4302535.358515/94‑1 (MQ=60)

                                                                            GTGAAACTTAAATGGCGCGCTGCGGCATCAAAAACTCGTAAGGCATTTAGCGGTGGTAATCGTTTAGACATGGCTATTAAACTTTGATGTTAAA                   <  SRR4302535.3745765/94‑1 (MQ=60)

                                                                              GAAACTTAAATGGCGCGCTGCGGCATCAAAAACTCGTAAGGCATTTAGCGGTGGTAATCGTTTAGACATGGCTATTAAACTTTGATGTTAAATG                 <  SRR4302535.3422073/94‑1 (MQ=60)

                                                                               AAACTTAAATGGCGCGCTGCGGCATCAAAAACTCGTAAGGCATTTAGCGGTGGTAATCGTTTAGACATGGCTATTAAACTTTGATGTTAAATGA                >  SRR4302535.1627305/1‑94 (MQ=60)

                                                                               AAACTTAAATGGCGCGCTGCGGCATCAAAAACTCGTAAGGCATTTAGCGGTGGTAATCGTTTAGACATGGCTATTAAACTTTGATGTTAAATGA                >  SRR4302535.2597789/1‑94 (MQ=60)

                                                                               AAACTTAAATGGCGCGCTGCGGCATCAAAAACTCGTAAGGCATTTAGCGGTGGTAATCGTTTAGACATGGCTATTAAACTTTGATGTTAAATGA                >  SRR4302535.4649361/1‑94 (MQ=60)

                                                                                AACTTAAATGGCGCGCTGCGGCATCAAAAACTCGTAAGGCATTTAGCGGTGGTAATCGTTTAGACATGGCTATTAAACTTTGATGTTAAATGAA               <  SRR4302535.3227912/94‑1 (MQ=60)

                                                                                 ACTTAAATGGCGCGCTGCGGCATCAAAAACTCGTAAGGCATTTAGCGGTGGTAATCGTTTAGACATGGCTATTAAACTTTGATGTTAAATGAAT              <  SRR4302535.1286520/94‑1 (MQ=60)

                                                                                  CTTAAATGGCGCGCTGCGGCATCAAAAACTCGTAAGGCATTTAGCGGTGGTAATCGTTTAGACATGGCTATTAAACTTTGATGTTAAATGAATT             >  SRR4302535.676645/1‑94 (MQ=60)

                                                                                      AATGGCGCGCTGCGGCATCAAAAACTCGTAAGGCATTTAGCGGTGGTAATCGTTTAGACATGGCTATTAAACTTTGATGTTAAATGAATTTAAC         <  SRR4302535.1479816/94‑1 (MQ=60)

                                                                                        TGGCGCGCTGCGGCATCAAAAACTCGTAAGGCATTTAGCGGTGGTAATCGTTTAGACATGGCTATTAAACTTTGATGTTAAATGAATTTAACAA       <  SRR4302535.2853855/94‑1 (MQ=60)

                                                                                        TGGCGCGCTGCGGCATCAAAAACTCGTAAGGCATTTAGCGGTGGTAATCGTTTAGACATGGCTATTAAACTTTGATGTTAAATGAATTTAACAA       <  SRR4302535.521505/94‑1 (MQ=60)

                                                                                        TGGAGCGCTGCGGCATCAAAAACTCGTAAGGCATTTAGCGGTGGTAATCGTTTAGACATGGCTATTAAACTTTGATGTTAAATGAATTTAACAA       <  SRR4302535.2073718/94‑1 (MQ=60)

                                                                                         GGCGCGCTGCGGCATCAAAAACTCGTAAGGCATTTAGCGGTGGTAATCGTTTAGACATGGCTATTAAACTTTGATGTTAAATGAATTTAACAAT      <  SRR4302535.4673168/94‑1 (MQ=60)

                                                                                         GGCGCGCTGCGGCATCAAAAACTCGTAAGGCATTTAGCGGTGGTAATCGTTTAGACATGGCTATTAAACTTTGATGTTAAATGAATTTAACAAT      <  SRR4302535.1623382/94‑1 (MQ=60)

                                                                                         GGCGCGCTGCGGCATCAAAAACTCGTAAGGCATTTAGCGGTAGTAATCGTTTAGACATGGCTATTAAACTTTGATGTTAAATGAATTTAACAAT      <  SRR4302535.1451435/94‑1 (MQ=60)

                                                                                            GCGCTGCGGCATCAAAAACTCGTAAGGCATTTAGCGGTGGTAATCGTTTAGACATGGCTATTAAACTTTGATGTTAAATGAATTTAACAATTAG   <  SRR4302535.4421426/94‑1 (MQ=60)

                                                                                             CGCTGCGGCATCAAAAACTCGTAAGGCATTTAGCGGTGGTAATCGTTTAGACATGGCTATTAAACTTTGATGTTAAATGAATTTAACAATTAGA  <  SRR4302535.1660895/94‑1 (MQ=60)

CCCCAAAAAATCCTCAAGAGACTTGATTTGATGACTTACTGCGGCTTGGGTCACAAAAAGCTCTTCTGCTGCGCGAGTGAAACTTAAATGGCGTGCTGCGGCATCAAAAACTCGTAAGGCATTTAGCGGTGGTAATCGTTTAGACATGGCTATTAAACTTTGATGTTAAATGAATTTAACAATTAGA  >  NC_000913/2940443‑2940629

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 20 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`