Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 1873 183.1 9376148 98.2% 9207377 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,977,219 T→C H59H (CAT→CAC)  lysR → LysR

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,977,2190TC100.0% 602.9 / NA 188H59H (CAT→CAC) lysRLysR
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (90/98);  total (90/98)

Only 100 of 193 total aligned reads displayed.

CACCTCACAGCCAACCGTCAGCCGCGAACTTGCGCGCTTTGAGAAGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACATCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATCCTGGTACGGACTGGATCGCATTGTCAGCGCCGCAGAAAGTCTGC  >  NC_000913/2977129‑2977307
                                                                                          |                                                                                        
cacCTCACAGCCAACCGTCAGCCGCGAACTTGCGCGCTTTGAGAAGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACAccc                                                                                        <  1:3872939/93‑1 (MQ=255)
cacCTCACAGCCAACCGTCAGCCGCGAACTTGCGCGCTTTGAGAAGGTGATCGGGCTGAAATTGTTTGAGCGCGCACGTGGGCGATTACAccc                                                                                        >  1:865682/1‑93 (MQ=255)
   cgcacaGCCAACCGTCAGCCGCGAACTTGCGCGCTTTGAGAAGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACACCCTAc                                                                                     <  1:4107895/91‑1 (MQ=255)
    tCACAGCCAACCGTCAGCCGCGAACTTGCGCGCTTTGAGAAGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACACCCTAcc                                                                                    >  1:1257039/1‑93 (MQ=255)
      ccaGCCAACCGTCAGCCGCGAACTTGCGCGCTTTGAGAAGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACACCCTACCGt                                                                                  <  2:2259397/92‑1 (MQ=255)
      acaGCCAACCGTCAGCCGCGAACTTGCGCGCTTTGAGAAGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACACCCTACCGt                                                                                  >  2:2710458/1‑93 (MQ=255)
         gCCAACCGTCAGCCGCGAACTTGCGCGCTTTGAGAAGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACACCCTACCGTGCa                                                                               >  1:377482/1‑93 (MQ=255)
          ccAACCGTCAGCCGCGAACTTGCGCGCTTTGAGAAGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACACCCTACCGTGcca                                                                              >  1:1724806/1‑91 (MQ=255)
           cAACCGTCAGCCGCGAACTTGCGCGCTTTGAGAAGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACACCCTACCGTGCAAg                                                                             <  2:4627979/93‑1 (MQ=255)
             aCCGTCAGCCGCGAACTTGCGCGCTTTGAGAAGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACACCCTACCGTGCAAGGa                                                                           >  2:324415/1‑93 (MQ=255)
               cGTCAGCCGCGAACTTGCGCGCTTTGAGAAGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACACCCTACCGTGCAAGGACt                                                                         <  2:3369525/93‑1 (MQ=255)
                 tCAGCCGCGAACTTGCGCGCTTTGAGAAGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACACCCTACCGTGCAAGGACTGc                                                                       >  1:2180969/1‑93 (MQ=255)
                   aGCCGCGAACTTGCGCGCTTTGAGAAGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACACCCTACCGTGCAAGGACTGCGt                                                                     <  1:3969485/93‑1 (MQ=255)
                    gCCGCGAACTTGCGCGCTTTGAGAAGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACACCCTACCGTGCAAGGACTGCGTc                                                                    <  2:1183461/93‑1 (MQ=255)
                    gCCGCGAACTTGCGCGCTTTGAGAAGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACACCCTACCGTGCAAGGACTGCGTc                                                                    <  2:1543440/93‑1 (MQ=255)
                      cgcgAACTTGCGCGCTTTGAGAAGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACACCCTACCGTGCAAGGACTGCGTCTg                                                                  >  1:3286826/1‑93 (MQ=255)
                       gcgAACTTGCGCGCTTTGAGAAGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACACCCTACCGTGCAAGGACTGCGTCTGt                                                                 <  1:186724/93‑1 (MQ=255)
                       gcgAACTTGCGCGCTTTGAGAAGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACACCCTACCGTGCAAGGACTGCGTCTGt                                                                 >  2:2475534/1‑93 (MQ=255)
                       gcgAACTTGCGCGCTTTGAGAAGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACACCCTACCGTGCAAGGACTGCGTCTGt                                                                 <  1:2900794/93‑1 (MQ=255)
                       gcgAACTTGCGCGCTTTGAGAAGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACACCCTACCGTGCAAGGACTGCGTCTGt                                                                 <  2:1017854/93‑1 (MQ=255)
                        cgAACTTGCGCGCTTTGAGAAGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACACCCTACCGTGCAAGGACTGCGTCTGtt                                                                <  1:2465546/93‑1 (MQ=255)
                         gAACTTGCGCGCTTTGAGAAGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACACCCTACCGTGCAAGGACTGCGTCTGttt                                                               <  1:2428936/93‑1 (MQ=255)
                         gAACTTGCGCGCTTTGAGAAGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACACCCTACCGTGCAAGGACTGCGTCTGttt                                                               >  1:4224868/1‑93 (MQ=255)
                             ttGCGCGCTTTGAGAAGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACACCCTACCGTGCAAGGACTGCGTCTGTTTgaag                                                           >  1:2522186/1‑93 (MQ=255)
                             ttGCGCGCTTTGAGAAGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACACCCTACCGTGCAAGGACTGCGTCTGTTTgaag                                                           <  2:51764/93‑1 (MQ=255)
                              tGCGCGCTTTGAGAAGGTGATCGGGCTGAAATTGTTTGAGCGCGTGCGTGGGCGATTACACCCTACCGTGCAAGGACTGCGTCTGTTTgaaga                                                          <  1:1447441/93‑1 (MQ=255)
                              tGCGCGCTTTGAGAAGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACACCCTACCGTGCAAGGACTGCGTCTGTTTgaaga                                                          <  2:3058013/93‑1 (MQ=255)
                               gcgcgcTTTGAGAAGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACACCCTACCGTGCAAGGACTGCGTCTGTTTgaagca                                                         >  1:4200924/1‑91 (MQ=255)
                               gcgcgcTTTGAGAAGGTGATCGGGCAGAAATTGTTTGAGCGCGTACGTGGGCGATTACACCCTACCGTGCAAGGACTGCGTCTGTTTgaagaa                                                         <  1:4527883/93‑1 (MQ=255)
                                cgcgcTTTGAGAAGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACACCCTACCGTGCAAGGACTGCGTCTGTTTGaagaag                                                        <  2:3538149/93‑1 (MQ=255)
                                cgcgcTTTGAGAAGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACACCCTACCGTGCAAGGACTGCGTCTGTTTGaagaag                                                        >  1:297040/1‑93 (MQ=255)
                                 gcgcTTTGAGAAGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACACCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGt                                                       <  2:1884792/93‑1 (MQ=255)
                                 gcgcTTTGAGAAGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACACCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGt                                                       <  1:3064431/93‑1 (MQ=255)
                                  cgcTTTGAGAAGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACACCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTg                                                      <  1:626239/93‑1 (MQ=255)
                                   gcTTTGAGAAGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACACCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGc                                                     <  1:4306823/93‑1 (MQ=255)
                                   gcTTTGAGAAGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACACCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGc                                                     >  1:3844109/1‑93 (MQ=255)
                                     tttGAGAAGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACACCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCaa                                                   <  2:489216/93‑1 (MQ=255)
                                     tttGAGAAGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACACCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCaa                                                   >  2:2550629/1‑93 (MQ=255)
                                     tttGAGAAGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACACCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCaa                                                   <  1:1308130/93‑1 (MQ=255)
                                     tttGAGAAGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACACCCTACCCTGCAAGGACTGCGTCTGTTTGTAGAAGTGGaa                                                   >  2:3285114/1‑93 (MQ=255)
                                      ttGAGAAGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACACCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAAc                                                  <  2:4413638/93‑1 (MQ=255)
                                        gagaAGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACACCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGa                                                <  2:588087/93‑1 (MQ=255)
                                        gagaAGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACACCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGa                                                >  2:1060125/1‑93 (MQ=255)
                                        gagaAGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACACCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGa                                                <  2:352600/93‑1 (MQ=255)
                                          gaAGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACACCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATc                                              >  1:3814733/1‑93 (MQ=255)
                                          gaAGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACACCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATc                                              >  2:3598125/1‑93 (MQ=255)
                                           aaGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACACCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATcc                                             <  2:4101744/93‑1 (MQ=255)
                                            aGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACACCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATCCt                                            >  1:4278771/1‑93 (MQ=255)
                                            aGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACACCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATCCt                                            >  1:4557796/1‑93 (MQ=255)
                                            aGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACACCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATCCt                                            <  1:2019412/93‑1 (MQ=255)
                                             ggcgATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACACCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATCATg                                           >  2:920438/4‑93 (MQ=255)
                                             ggTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACACCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATCCTg                                           <  1:4080319/93‑1 (MQ=255)
                                               tGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACACCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATCCTGGt                                         <  1:3581270/93‑1 (MQ=255)
                                                 aTCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACACCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATCCTGGTAc                                       <  1:2405102/93‑1 (MQ=255)
                                                  tCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACACCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATCCTGGTACg                                      >  1:1052149/1‑93 (MQ=255)
                                                  tCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACACCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATCCTGGTACg                                      <  1:2150204/93‑1 (MQ=255)
                                                     ggCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACACCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATCCTGGTACGGAc                                   <  2:3908473/93‑1 (MQ=255)
                                                         gAAATTGTTTGAGCGCGTACGTGGGCGATTACACCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATCCTGGTACGGACTGGa                               <  2:3016682/93‑1 (MQ=255)
                                                          aaaTTGTTTGAGCGCGTACGTGGGCGATTACACCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATCCTGGTACGGACTGGAt                              <  1:2761077/93‑1 (MQ=255)
                                                          aaaTTGTTTGAGCGCGTACGTGGGCGATTACACCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATCCTGGTACGGACTGGAt                              <  1:2850625/93‑1 (MQ=255)
                                                            aTTGTTTGAGCGCGTACGTGGGCGATTACACCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATCCTGGTACGGACTGGATCg                            <  1:524164/93‑1 (MQ=255)
                                                            aTTGTTTGAGCGCGTACGTGGGCGATTACACCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATCCTGGTACGGACTGGATCg                            <  1:1335514/93‑1 (MQ=255)
                                                            aTTGTTTGAGCGCGTACGTGGGCGATTACACCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATCCTGGTACGGACTGGATCg                            >  1:3189827/1‑93 (MQ=255)
                                                             ttGTTTGAGCGCGTACGTGGGCGATTACACCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATCCTGGTACGGACTGGATCGc                           <  1:652537/93‑1 (MQ=255)
                                                             ttGTTTGAGCGCGTACGTGGGCGATTACACCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATCCTGGTACGGACTGGATCGc                           <  2:2219945/93‑1 (MQ=255)
                                                             ttGTTTGAGCGCGTACGTGGGCGATTACACCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATCCTGGTACGGACTGGATCGc                           >  1:3388581/1‑93 (MQ=255)
                                                              tGTTTGAGCGCGTACGTGGGCGATTACACCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATCCTGGTACGGACTGGATCGCa                          >  2:974412/1‑93 (MQ=255)
                                                              tGTTTGAGCGCGTACGTGGGCGATTACACCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATCCTGGTACGGACTGGATCGCa                          <  1:487953/93‑1 (MQ=255)
                                                               gTTTGAGCGCGTACGTGGGCGATTACACCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATCCTGGTACGGACTGGATCGCAt                         >  2:1942000/1‑93 (MQ=255)
                                                               gTTTGAGCGCGTACGTGGGCGATTACACCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATCCTGGTACGGACTGGATCGCAt                         >  1:182037/1‑93 (MQ=255)
                                                                  tGAGCGCGTACGTGGGCGATTACACCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATCCTGGTACGGACTGGATCGCATTGt                      >  2:1910245/1‑93 (MQ=255)
                                                                  tGAGCGCGTACGTGGGCGATTACACCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATCCTGGTACGGACTGGATCGCATTGt                      <  1:4589428/93‑1 (MQ=255)
                                                                  tGAGCGCGTACGTGGGCGATTACACCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATCCTGGTACGGACTGGATCGCATTGt                      <  1:2091829/93‑1 (MQ=255)
                                                                  tGAGCGCGTACGTGGGCGATTACACCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATCCTGGTACGGACTGGATCGCATTGt                      <  1:3714767/93‑1 (MQ=255)
                                                                    aGCGCGTACGTGGGCGATTACACCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATCCTGGTACGGACTGGATCGCATTGTCa                    <  2:655208/93‑1 (MQ=255)
                                                                     gcgcGTACGTGGGCGATTACACCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATCCTGGTACGGACTGGATCGCATTGTCAg                   <  1:1010063/93‑1 (MQ=255)
                                                                      cgcgTACGTGGGCGATTACACCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATCCTGGTACGGACTGGATCGCATTGTCAgc                  >  2:4341791/1‑93 (MQ=255)
                                                                        cgTACGTGGGCGATTACACCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATCCTGGTACGGACTGGATCGCATTGTCAgcgc                >  1:730153/1‑93 (MQ=255)
                                                                         gTACGTGGGCGATTACACCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATCCTGGTACGGACTGGATCGCATTGTCAGcgcc               <  2:2442244/93‑1 (MQ=255)
                                                                         gTACGTGGGCGATTACACCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATCCTGGTACGGACTGGATCGCATTGTCAGcgcc               <  1:4047383/93‑1 (MQ=255)
                                                                         gTACGTGGGCGATTACACCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATCCTGGTACGGACTGGATCGCATTGTCAGcgcc               >  2:2849275/1‑93 (MQ=255)
                                                                         gTACGTGGGCGATTACACCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATCCTGGTACGGACTGGATCGCATTGTCAGcgcc               <  1:4474126/93‑1 (MQ=255)
                                                                          tACGTGGGCGATTACACCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATCCTGGTACGGACTGGATCGCATTGTCAGcgccg              >  1:1236861/1‑93 (MQ=255)
                                                                          tACGTGGGCGATTACACCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATCCTGGTACGGACTGGATCGCATTGTCAGcgccg              >  2:6645/1‑93 (MQ=255)
                                                                          tACGTGGGCGATTACACCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATCCTGGTACGGACTGGATCGCATTGTCAGcgccg              >  1:216341/1‑93 (MQ=255)
                                                                          tACGTGGGCGATTACACCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATCCTGGTACGGACTGGATCGCATTGTCAGcgacg              >  2:2313335/1‑93 (MQ=255)
                                                                           aCGTGGGCGATTACACCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATCCTGGTACGGACTGGATCGCATTGTCAGcgccgc             >  2:3449625/1‑93 (MQ=255)
                                                                            cGTGGGCGATTACACCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATCCTGGTACGGACTGGATCGCATTGTCAGCGCCGCa            <  2:3980389/93‑1 (MQ=255)
                                                                             gTGGGCGATTACACCCTACCGTGCAAGGTCTGCGTCTGTTTGAAGAAGTGCAAGGATCTTGGTACGCATCGGATAATATTTTCAGctccgcca           >  2:449573/1‑91 (MQ=255)
                                                                              tGGGCGATTACACCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATCCTGGTACGGACTGGATCGCATTGTCAGCGCCGCAGa          <  2:443102/93‑1 (MQ=255)
                                                                               gggCGATTACACCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATCCTGGTACGGACTGGATCGCATTGTCAGCGCCGCAGaa         >  2:4007017/1‑93 (MQ=255)
                                                                                ggCGATTACACCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATCCTGGTACGGACTGGATCGCATTGTCAGCGCCGCAGaaa        >  1:4328755/1‑93 (MQ=255)
                                                                                ggCGATTACACCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATCCTGGTACGGACTGGATCGCATTGTCAGCGCCGCAGaaa        <  1:1574281/93‑1 (MQ=255)
                                                                                ggCGATTACACCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATCCTGGTACGGACTGGATCGCATTGTCAGCGCCGCAGaaa        >  1:3122408/1‑93 (MQ=255)
                                                                                 gCGATTACACCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATCCTGGTACGGACTGGATCGCATTGTCAGCGCCGCAGAAAg       <  1:3241548/93‑1 (MQ=255)
                                                                                   gATTACACCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATCCTGGTACGGACTGGATCGCATTGTCAGCGCCGCAGAAAGTc     <  1:1535394/93‑1 (MQ=255)
                                                                                   gATTACACCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATCCTGGTACGGACTGGATCGCATTGTCAGCGCCGCAGAAAGTc     >  2:160511/1‑93 (MQ=255)
                                                                                    aTTACACCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGAGCAACGATCCTGGTACGGACTGGATCGCATTGTCAGCGCCGCAGAAAGTCt    >  2:3412431/1‑93 (MQ=255)
                                                                                     ttACACCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATCCTGGTACGGACTGGATCGCATTGTCAGCGCCGCAGAAAGTCTg   <  2:4017524/93‑1 (MQ=255)
                                                                                      tACACCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATCCTGGTACGGACTGGATCGCATTGTCAGCGCCGCAGAAAGTCTgc  <  1:2905967/93‑1 (MQ=255)
                                                                                          |                                                                                        
CACCTCACAGCCAACCGTCAGCCGCGAACTTGCGCGCTTTGAGAAGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACATCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATCCTGGTACGGACTGGATCGCATTGTCAGCGCCGCAGAAAGTCTGC  >  NC_000913/2977129‑2977307

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
ACACACCTCACAGCCAACCGTCAGCCGCGAACTTGCGCGCTTTGAGAAGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACATCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATCCTGGTACGGACTGGATCGCATTGTCAGCGCCGCAGAAAGTCTGCGCGAG  >  NC_000913/2977126‑2977312
                                                                                             |                                                                                             
ACACACCTCACAGCCAACCGTCAGCCGCGAACTTGCGCGCTTTGAGAAGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACAC                                                                                               <  SRR4302535.3811908/94‑1 (MQ=60)
ACACACCTCACAGCCAACCGTCAGCCGCGAACTTGCGCGCTTTGAGAAGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACAC                                                                                               <  SRR4302535.3690184/94‑1 (MQ=60)
  ACACCTCACAGCCAACCGTCAGCCGCGAACTTGCGCGCTTTGAGAAGGTGATCGGGCTGAAATTGTTTGAGCGCGCACGTGGGCGATTACACCC                                                                                             >  SRR4302535.871797/1‑94 (MQ=60)
   CACCTCACAGCCAACCGTCAGCCGCGAACTTGCGCGCTTTGAGAAGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACACCCT                                                                                            <  SRR4302535.3899553/94‑1 (MQ=60)
    ACCTCACAGCCAACCGTCAGCCGCGAACTTGCGCGCTTTGAGAAGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACACCCTA                                                                                           <  SRR4302535.1330595/94‑1 (MQ=60)
     CCTCACAGCCAACCGTCAGCCGCGAACTTGCGCGCTTTGAGAAGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACACCCTAC                                                                                          >  SRR4302535.3801080/1‑94 (MQ=60)
      CTCACAGCCAACCGTCAGCCGCGAACTTGCGCGCTTTGAGAAGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACACCCTACC                                                                                         >  SRR4302535.1266031/1‑94 (MQ=60)
      CTCACAGCCAACCGTCAGCCGCGAACTTGCGCGCTTTGAGAAGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACACCCTACC                                                                                         >  SRR4302535.648272/1‑94 (MQ=60)
      CGCACAGCCAACCGTCAGCCGCGAACTTGCGCGCTTTGAGAAGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACACCCTACC                                                                                         <  SRR4302535.4136022/94‑1 (MQ=60)
         ACAGCCAACCGTCAGCCGCGAACTTGCGCGCTTTGAGAAGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACACCCTACCGTG                                                                                      <  SRR4302535.134737/94‑1 (MQ=60)
           AGCCAACCGTCAGCCGCGAACTTGCGCGCTTTGAGAAGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACACCCTACCGTGCA                                                                                    >  SRR4302535.380101/1‑94 (MQ=60)
           AGCCAACCGTCAGCCGCGAACTTGCGCGCTTTGAGAAGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACACCCTACCGTGCA                                                                                    <  SRR4302535.2725050/94‑1 (MQ=60)
            GCCAACCGTCAGCCGCGAACTTGCGCGCTTTGAGAAGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACACCCTACCGTGCCA                                                                                   >  SRR4302535.1737280/1‑94 (MQ=60)
             CCAACCGTCAGCCGCGAACTTGCGCGCTTTGAGAAGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACACCCTACCGTGCAAG                                                                                  <  SRR4302535.3863065/94‑1 (MQ=60)
               AACCGTCAGCCGCGAACTTGCGCGCTTTGAGAAGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACACCCTACCGTGCAAGGA                                                                                >  SRR4302535.1607790/1‑94 (MQ=60)
                ACCGTCAGCCGCGAACTTGCGCGCTTTGAGAAGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACACCCTACCGTGCAAGTAC                                                                               >  SRR4302535.1284076/1‑94 (MQ=60)
                ACCGTCAGCCGCGAACTTGCGCGCTTTGAGAAGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACACCCTACCGTGCAAGGAC                                                                               <  SRR4302535.4064585/94‑1 (MQ=60)
                ACCGTCAGCCGCGAACTTGCGCGCTTTGAGAAGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACACCCTACCGTGCAAGGAC                                                                               <  SRR4302535.4308942/94‑1 (MQ=60)
                 CCGTCAGCCGCGAACTTGCGCGCTTTGAGAAGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACACCCTACCGTGCAAGGACT                                                                              <  SRR4302535.430785/94‑1 (MQ=60)
                   GTCAGCCGCGAACTTGCGCGCTTTGAGAAGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACACCCTACCGTGCAAGGACTGC                                                                            >  SRR4302535.2196530/1‑94 (MQ=60)
                      AGCCGCGAACTTGCGCGCTTTGAGAAGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACACCCTACCGTGCAAGGACTGCGTC                                                                         <  SRR4302535.3996598/94‑1 (MQ=60)
                      AGCCGCGAACTTGCGCGCTTTGAGAAGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACACCCTACCGTGCAAGGACTGCGTC                                                                         >  SRR4302535.4099962/1‑94 (MQ=60)
                        CCGCGAACTTGCGCGCTTTGAGAAGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACACCCTACCGTGCAAGGACTGCGTCTG                                                                       >  SRR4302535.3309675/1‑94 (MQ=60)
                         CGCGAACTTGCGCGCTTTGAGAAGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACACCCTACCGTGCAAGGACTGCGTCTGT                                                                      >  SRR4302535.2063498/1‑94 (MQ=60)
                          GCGAACTTGCGCGCTTTGAGAAGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACACCCTACCGTGCAAGGACTGCGTCTGTT                                                                     <  SRR4302535.2921297/94‑1 (MQ=60)
                          GCGAACTTGCGCGCTTTGAGAAGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACACCCTACCGTGCAAGGACTGCGTCTGTT                                                                     <  SRR4302535.188012/94‑1 (MQ=60)
                           CGAACTTGCGCGCTTTGAGAAGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACACCCTACCGTGCAAGGACTGCGTCTGTTT                                                                    <  SRR4302535.2483323/94‑1 (MQ=60)
                           CGAACTTGCGCGCTTTGAGAAGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACACCCTACCGTGCAAGGACTGCGTCTGTTT                                                                    >  SRR4302535.4253776/1‑94 (MQ=60)
                            GAACTTGCGCGCTTTGAGAAGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACACCCTACCGTGCAAGGACTGCGTCTGTTTG                                                                   <  SRR4302535.2446437/94‑1 (MQ=60)
                             AACTTGCGCGCTTTGAGAAGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACACCCTACCGTGCAAGGACTGCGTCTGTTTGA                                                                  >  SRR4302535.2925773/1‑94 (MQ=60)
                             AACTTGCGCGCTTTGAGAAGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACACCCTACCGTGCAAGGACTGCGTCTGTTTGA                                                                  >  SRR4302535.2909252/1‑94 (MQ=60)
                               CTTGCGCGCTTTGAGAAGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACACCCTACCGTGCAAGGACTGCGTCTGTTTGAAG                                                                >  SRR4302535.2540361/1‑94 (MQ=60)
                                 TGCGCGCTTTGAGAAGGTGATCGGGCTGAAATTGTTTGAGCGCGTGCGTGGGCGATTACACCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAA                                                              <  SRR4302535.1457791/94‑1 (MQ=60)
                                 TGCGCGCTTTGAGAAGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACACCCTACCGTGCAAGGACTGCGTCTGTTTGAAGCA                                                              >  SRR4302535.4229671/1‑94 (MQ=60)
                                  GCGCGCTTTGAGAAGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACACCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAG                                                             >  SRR4302535.299110/1‑94 (MQ=60)
                                  GCGCGCTTTGAGAAGGTGATCGGGCAGAAATTGTTTGAGCGCGTACGTGGGCGATTACACCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAG                                                             <  SRR4302535.4558893/94‑1 (MQ=60)
                                    GCGCTTTGAGAAGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACACCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTG                                                           <  SRR4302535.3085851/94‑1 (MQ=60)
                                     CGCTTTGAGAAGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACACCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGC                                                          >  SRR4302535.3870585/1‑94 (MQ=60)
                                     CGCTTTGAGAAGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACACCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGC                                                          <  SRR4302535.630596/94‑1 (MQ=60)
                                      GCTTTGAGAAGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACACCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCA                                                         <  SRR4302535.4336288/94‑1 (MQ=60)
                                      GCTTTGAGAAGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACACCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCA                                                         >  SRR4302535.3883983/1‑94 (MQ=60)
                                      GCTTTGAGAAGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACACCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCA                                                         >  SRR4302535.526177/1‑94 (MQ=60)
                                       CTTTGAGAAGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACACCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAA                                                        >  SRR4302535.3182802/1‑94 (MQ=60)
                                        TTTGAGAAGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTCCACCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAAC                                                       <  SRR4302535.2537558/94‑1 (MQ=60)
                                        TTTGAGAAGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACACCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAAC                                                       <  SRR4302535.1317473/94‑1 (MQ=60)
                                            AGAAGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACACCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATC                                                   >  SRR4302535.3841055/1‑94 (MQ=60)
                                              GAGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACACCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATCCT                                                 <  SRR4302535.1098853/94‑1 (MQ=60)
                                              AAGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACACCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATCCT                                                 >  SRR4302535.1264437/1‑94 (MQ=60)
                                              AAGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACACCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATCCT                                                 >  SRR4302535.4308051/1‑94 (MQ=60)
                                              AAGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACACCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATCCT                                                 >  SRR4302535.4588916/1‑94 (MQ=60)
                                               AGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACACCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATCCTG                                                <  SRR4302535.2033969/94‑1 (MQ=60)
                                                GGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACACCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATCCTGG                                               <  SRR4302535.1878494/94‑1 (MQ=60)
                                                GGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACACCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATCCTGG                                               <  SRR4302535.4108229/94‑1 (MQ=60)
                                                GGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACACCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATCCTGG                                               <  SRR4302535.2869493/94‑1 (MQ=60)
                                                  TGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACACCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATCCTGGTA                                             >  SRR4302535.418945/1‑94 (MQ=60)
                                                  TGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACACCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATCCTGGTA                                             <  SRR4302535.3606109/94‑1 (MQ=60)
                                                    ATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACACCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATCCTGGTACG                                           >  SRR4302535.1059632/1‑94 (MQ=60)
                                                    ATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACACCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATCCTGGTACG                                           <  SRR4302535.2422411/94‑1 (MQ=60)
                                                     TCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACACCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATCCTGGTACGG                                          <  SRR4302535.2165666/94‑1 (MQ=60)
                                                        GGCTGAAAGTGTGTGAGCGCGTAGGTGGGCGATGACACACGACCGTGCAAGGACTGCGTCGGTTTGAAGAAGTGCAACGATCCTGGTACGGACT                                       <  SRR4302535.2239182/94‑1 (MQ=60)
                                                         GCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACACCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATCCTGGTACGGACTG                                      >  SRR4302535.2125536/1‑94 (MQ=60)
                                                         GCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACACCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATCCTGGTACGGACTG                                      >  SRR4302535.374221/1‑94 (MQ=60)
                                                          CTGAAATTGTTTGAGCGCGTACGTGGGCGATTACACCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATCCTGGTACGGACTGG                                     >  SRR4302535.487275/1‑94 (MQ=60)
                                                          CTGAAATTGTTTGAGCGCGTACGTGGGCGATTACACCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATCCTGGTACGGACTGG                                     >  SRR4302535.1542673/1‑94 (MQ=60)
                                                           TGAAATTGTTTGAGCGCGTACGTGGGCGATTACACCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATCCTGGTACGGACTGGA                                    >  SRR4302535.4355301/1‑94 (MQ=60)
                                                           TGAAATTGTTTGAGCGCGTACGTGGGCGATTACACCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATCCTGGTACGGACTGGA                                    >  SRR4302535.2187406/1‑94 (MQ=60)
                                                           TGAAATTGTTTGAGCGCGTACGTGGGCGATTACACCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATCCTGGTACGGACTGGA                                    >  SRR4302535.3060333/1‑94 (MQ=60)
                                                             AAATTGTTTGAGCGCGTACGTGGGCGATTACACCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATCCTGGTACGGACTGGATC                                  <  SRR4302535.2780752/94‑1 (MQ=60)
                                                             AAATTGTTTGAGCGCGTACGTGGGCGATTACACCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATCCTGGTACGGACTGGATC                                  <  SRR4302535.2870825/94‑1 (MQ=60)
                                                              AATTGTTTGAGCGCGTACGTGGGCGATTACACCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATCCTGGTACGGACTGGATCG                                 >  SRR4302535.2475238/1‑94 (MQ=60)
                                                              AATTGTTTGAGCGCGTACGTGGGCGATTACACCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATCCTGGTACGGACTGGATCG                                 >  SRR4302535.3211888/1‑94 (MQ=60)
                                                               ATTGTTTGAGCGCGTACGTGGGCGATTACACCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATCCTGGTACGGACTGGATCGC                                <  SRR4302535.1345041/94‑1 (MQ=60)
                                                               ATTGTTTGAGCGCGTACGTGGGCGATTACACCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATCCTGGTACGGACTGGATCGC                                <  SRR4302535.527826/94‑1 (MQ=60)
                                                               ATTGTTTGAGCGCGTACGTGGGCGATTACACCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATCCTGGTACGGACTGGATCGC                                >  SRR4302535.3412155/1‑94 (MQ=60)
                                                                TTGTTTGAGCGCGTACGTGGGCGATTACACCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATCCTGGTACGGACTGGATCGCA                               <  SRR4302535.657088/94‑1 (MQ=60)
                                                                 TGTTTGAGCGCGTACGTGGGCGATTACACCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATCCTGGTACGGACTGGATCGCAT                              <  SRR4302535.491373/94‑1 (MQ=60)
                                                                 TGTTTGAGCGCGTACGTGGGCGATTACACCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATCCTGGTACGGACTGGATCGCAT                              >  SRR4302535.183297/1‑94 (MQ=60)
                                                                  GTTTGAGCGCGTACGTGGGCGATTACACCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATCCTGGTACGGACTGGATCGCATT                             <  SRR4302535.3644160/94‑1 (MQ=60)
                                                                   TTTGAGCGCGTACGTGGGCGATTACACCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATCCTGGTACGGACTGGATCGCATTG                            <  SRR4302535.3570538/94‑1 (MQ=60)
                                                                   TGTGAGCGCGTACGTGGGCGATTACACCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATCCTGGTACGGACTGGATCGCATTG                            <  SRR4302535.3112541/94‑1 (MQ=60)
                                                                     TGAGCGCGTACGTGGGCGATTACACCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATCCTGGTACGGACTGGATCGCATTGTC                          <  SRR4302535.4620672/94‑1 (MQ=60)
                                                                     TGAGCGCGTACGTGGGCGATTACACCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATCCTGGTACGGACTGGATCGCATTGTC                          <  SRR4302535.3740501/94‑1 (MQ=60)
                                                                     TGAGCGCGTACGTGGGCGATTACACCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATCCTGGTACGGACTGGATCGCATTGTC                          <  SRR4302535.2106903/94‑1 (MQ=60)
                                                                        GCGCGTACGTGGGCGATTACACCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATCCTGGTACGGACTGGATCGCATTGTCAGC                       <  SRR4302535.1017240/94‑1 (MQ=60)
                                                                         CGCGTACGTGGGCGATTACACCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATCCTGGTACGGACTGGATCGCATTGTCAGCG                      <  SRR4302535.4608871/94‑1 (MQ=60)
                                                                         CGCGTACGTGGGCGATTACACCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATCCTGGTACGGACTGGATCGCATTGTCAGCG                      >  SRR4302535.3289755/1‑94 (MQ=60)
                                                                          GCGTACGTGGGCGATTACACCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATCCTGGTACGGACTGGATCGCATTGTCAGCGC                     >  SRR4302535.735280/1‑94 (MQ=60)
                                                                            GTACGTGGGCGATTACACCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATCCTGGTACGGACTGGATCGCATTGTCAGCGCCG                   <  SRR4302535.1798507/94‑1 (MQ=60)
                                                                            GTACGTGGGCGATTACACCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATCCTGGTACGGACTGGATCGCATTGTCAGCGCCG                   >  SRR4302535.1245695/1‑94 (MQ=60)
                                                                            GTACGTGGGCGATTACACCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATCCTGGTACGGACTGGATCGCATTGTCAGCGCCG                   <  SRR4302535.4075088/94‑1 (MQ=60)
                                                                            GTACGTGGGCGATTACACCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATCCTGGTACGGACTGGATCGCATTGTCAGCGCCG                   >  SRR4302535.217825/1‑94 (MQ=60)
                                                                            GTACGTGGGCGATTACACCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATCCTGGTACGGACTGGATCGCATTGTCAGCGCCG                   <  SRR4302535.4504827/94‑1 (MQ=60)
                                                                             TACGTGGGCGATTACACCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATCCTGGTACGGACTGGATCGCATTGTCAGCGCCGC                  >  SRR4302535.3922914/1‑94 (MQ=60)
                                                                              ACGTGGGCGATTACACCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCCACGATCCTGGTACGGACTGGATCGCATTGTCAGCGCCGCG                 >  SRR4302535.4532005/1‑94 (MQ=60)
                                                                               CGTGGGCGATTACACCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATCCTGGTACGGACTGGATCGCCTTGTCAGCGCCGCAG                >  SRR4302535.1921935/1‑94 (MQ=60)
                                                                               CGTGGGCGATTACACCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATCCTGGTACGGACTGGATCGCATTGTCAGCGCCGCAG                >  SRR4302535.622748/1‑94 (MQ=60)
                                                                                  GGGCGATTACACCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATCCTGGTACGGACTGGATCGCATTGTCAGCGCCGCAGAAA             >  SRR4302535.3144153/1‑94 (MQ=60)
                                                                                  GGGCGATTACACCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATCCTGGTACGGACTGGATCGCATTGTCAGCGCCGCAGAAA             >  SRR4302535.4358354/1‑94 (MQ=60)
                                                                                   GGCGATTACACCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATCCTGGTACGGACTGGATCGCATTGTCAGCGCCGCAGAAAG            <  SRR4302535.1580583/94‑1 (MQ=60)
                                                                                   GGCGATTACACCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATCCTGGTACGGACTGGATCGCATTGTCAGCGCCGCAGAAAG            <  SRR4302535.1585646/94‑1 (MQ=60)
                                                                                   GGCGATTACACCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATCCTGGTACGGACTGGATCGCATTGTCAGCGCCGCAGAAAG            >  SRR4302535.697304/1‑94 (MQ=60)
                                                                                    GCGATTACACCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATCCTGGTACGGACTGGATCGCATTGTCAGCGCCGCAGAAAGT           <  SRR4302535.4571608/94‑1 (MQ=60)
                                                                                    GCGATTACACCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATCCTGGTACGGACTGGATCGCATTGTCAGCGCCGCAGAAAGT           <  SRR4302535.3264062/94‑1 (MQ=60)
                                                                                     CGATTACACCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATCCTGGTACGGACTGGATCGCATTGTCAGCGCCGCAGAAAGTC          <  SRR4302535.4125038/94‑1 (MQ=60)
                                                                                      GATTACACCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATCCTGGTACGGACTGGATCGCATTGTCAGCGCCGCAGAAAGTCT         <  SRR4302535.3410316/94‑1 (MQ=60)
                                                                                      GATTACACCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATCCTGGTACGGACTGGATCGCATTGTCAGCGCCGCAGAAAGTCT         <  SRR4302535.1546455/94‑1 (MQ=60)
                                                                                        TTACACCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGAGCAACGATCCTGGTACGGACTGGATCGCATTGTCAGCGGCGCAGAAAGTCTGC       >  SRR4302535.2929571/1‑94 (MQ=60)
                                                                                         TACACCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATCCTGGTACGGACTGGATCGCATTGTCAGCGCCGCAGAAAGTCTGCG      <  SRR4302535.437367/94‑1 (MQ=60)
                                                                                         TACACCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATCCTGGTACGGACTGGATCGCATTGTCAGCGCCGCAGAAAGTCTGCG      <  SRR4302535.2427546/94‑1 (MQ=60)
                                                                                         TACACCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATCCTGGTACGGACTGGATCGCATTGTCAGCGCCGCAGAAAGTCTGCG      <  SRR4302535.802585/94‑1 (MQ=60)
                                                                                         TACACCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATCCTGGTACGGACTGGATCGCATTGTCAGCGCCGCAGAAAGTCTGCG      <  SRR4302535.2926507/94‑1 (MQ=60)
                                                                                           CACCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATCCTGGTACGGACTGGATCGCATTGTCAGCGCCGCAGAAAGTCTGCGCG    >  SRR4302535.4656032/1‑94 (MQ=60)
                                                                                           CACCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATCCTGGTACGGACTGGATCGCATTGTCAGCGCCGCAGAAAGTCTGCGCG    >  SRR4302535.4340925/1‑94 (MQ=60)
                                                                                           CACCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATCCTGGTACGGACTGGATCGCATTGTCAGCGCCGCAGAAAGTCTGCGCG    >  SRR4302535.4018953/1‑94 (MQ=60)
                                                                                            ACCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATCCTGGTACGGACTGGATCGCATTGTCAGCGCCGCAGAAAGTCTGCGCGA   >  SRR4302535.4657450/1‑94 (MQ=60)
                                                                                            ACCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATCCTGGTACGGACTGGATCGCATTGTCAGCGCCGCAGAAAGTCTGCGCGA   >  SRR4302535.3916171/1‑94 (MQ=60)
                                                                                             CCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATCCTGGTACGGACTGGATCGCATTGTCAGCGCCGCAGAAAGTCTGCGCGAG  >  SRR4302535.1482481/1‑94 (MQ=60)
                                                                                             |                                                                                             
ACACACCTCACAGCCAACCGTCAGCCGCGAACTTGCGCGCTTTGAGAAGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACATCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATCCTGGTACGGACTGGATCGCATTGTCAGCGCCGCAGAAAGTCTGCGCGAG  >  NC_000913/2977126‑2977312

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑