Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I2 R1	1873	183.1	9376148	98.2%	9207377	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,152,247	+C	intergenic (+10/+29)	yghB → / ← yqhC	hypothetical protein/putative ARAC‑type regulatory protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,152,244	1	.	C	99.4%	529.5 / ‑3.8	166	intergenic (+7/+32)	yghB/yqhC	hypothetical protein/putative ARAC‑type regulatory protein
Reads supporting (aligned to +/- strand): ref base . (1/0); new base C (106/59); total (107/59)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.96e-01

Only 100 of 198 total aligned reads displayed.

TGATGATCCTGCCAATTGCCTTGTTAACCGCTGGCTTGTTAGGCACGCTGTTTGTGGTGATTAAAAAAAAATACTGTAACGCCTGACGAT‑TTT‑CCC‑‑GTTCCCGGTTGCTGTACCGGGAACGTATTTAATTCCCCTGCATCGCCCGCATTCTTGCCGCATCTTCCCCCGGCGTCACACCGAA  >  NC_000913/3152152‑3152332

tgatgaTCCTGCCAATTGCCTTGTTAACCGCTGGCTTGTTAGGCACGCTGTTTGTGGTGATTAAAAAAAAATACTGTAACGCCTGACGAt‑ttt                                                                                             >  1:3843937/1‑93 (MQ=255)

tgatgaTCCTGCCAATTGCCTTGTTAACCGCTGGCTTGTTAGGCACGCTGTTTGTGGTGATTAAAAAAAAATACTGTAACGCCTGACGAt‑ttt                                                                                             >  1:3940926/1‑93 (MQ=255)

 gatgatCCTGCCAATTGCCTTGTTAACCGCTGGCTTGGTAGGCACGCTGTTTGTGGTGATTAAAAAAAAATACTGTAACGCCTGAGGAT‑Ttt‑c                                                                                           >  2:2819139/1‑93 (MQ=255)

   tgatCCTGCCAATTGCCTTGTTAACCGCTGGCTTGTTAGGCACGCTGTTTGTGGTGATTAAAAAAAAATACTGTAACGCCTGACGAT‑Ttt‑ccc                                                                                         <  1:3620705/93‑1 (MQ=255)

   tgatCCTGCCAATTGCCTTGTTAACCGCTGGCTTGTTAGGCACGCTGTTTGTGGTGATTAAAAAAAAATACTGTAACGCCTGACGAT‑Ttt‑ccc                                                                                         >  2:4365894/1‑93 (MQ=255)

   tgatCCTGCCAATTGCCTTGTTAACCGCTGGCTTGTTAGGCACGCTGTTTGTGGTGATTAAAAAAAAATACTGTAACGCCTGACGAT‑Ttt‑ccc                                                                                         >  1:4357498/1‑93 (MQ=255)

    gatCCTGCCAATTGCCTTGTTAACCGCTGGCTTGTTAGGCACGCTGTTTGTGGTGATTAAAAAAAAATACTGTAACGCCTGACGAT‑Ttt‑cccc                                                                                        >  1:4402806/1‑92 (MQ=255)

    gatCCTGCCAATTGCCTTGTTAACCGCTGGCTTGTTAGGCACGCTGTTTGTGGTGATTAAAAAAAAATACTGTAACGCCTGACGAT‑Ttt‑cccc                                                                                        <  2:4444581/93‑2 (MQ=255)

     atCCTGCCAATTGCCTTGTTAACCGCTGGCTTGTTAGGCACGCTGTTTGTGGTGATTAAAAAAAAATACTGTAACGCCTGACGAT‑Ttt‑ccccg                                                                                       <  2:3935952/93‑3 (MQ=255)

      tCCTGCCAATTGCCTTGTTAACCGCTGGCTTGTTAGGCACGCTGTTTGTGGTGATTAAAAAAAAATACTGTAACGCCTGACGAT‑Ttt‑ccccgt                                                                                      >  2:3023515/1‑90 (MQ=255)

         tGCCAATTGCCTTGTTAACCGCTGGCTTGTTAGGCACGCTGTTTGTGGTGATTAAAAAAAAATACTGTAACGCCTGACGAT‑TTtcccc‑‑gttcc                                                                                  >  2:3694614/1‑93 (MQ=255)

         tGCCAATTGCCTTGTTAACCGCTGGCTTGTTAGGCACGCTGTTTGTGGTGATTAAAAAAAAATACTGTAACGCCTGACGAT‑TTtcccc‑‑gttcc                                                                                  <  1:887328/93‑1 (MQ=255)

             aaTTGCCTTGTTAACCGCTGGCTTGTTAGGCACGCTGTTTGTGGTGATTAAAAAAAAATACTGTAACGCCTGACGAT‑TTTCCCC‑‑GTTCCCGGt                                                                              <  2:2235226/93‑1 (MQ=255)

              aTTGCCTTGTTAACCGCTGGCTTGTTAGGCACGCTGTTTGTGGTGATTAAAAAAAAATACTGTAACGCCTGACGAT‑TTTCCCC‑‑GTTCCCGGtt                                                                             >  1:283793/1‑93 (MQ=255)

              aTTGCCTTGTTAACCGCTGGCTTGTTAGGCACGCTGTTTGTGGTGATTAAAAAAAAATACTGTAACGCCTGACGAT‑TTTCCCC‑‑GTTCCCGGtt                                                                             <  2:1577110/93‑1 (MQ=255)

                tGCGTTGTTAACCGCTGTCTTGTTAGGCACGCTGTTTGTGGTGATTAAAAAAAAATACTGTAACGCCTGACGAT‑TTTCCCC‑‑GTTCCCGGTTGc                                                                           <  2:468534/93‑1 (MQ=255)

                tGCCTTGTTAACCGCTGGCTTGTTAGGCACGCTGTTTGTGGTGATAAAAAAAAAATACTGTAACGCCTGACGAT‑TTTCCCC‑‑GTTCCCGGTTGc                                                                           <  2:149318/93‑1 (MQ=255)

                 gCCTTGTTAACCGCTGGCTTGTTAGGCACGCTGTTTGTGGTGATTAAAAAAAAATACTGTAACGCCTGACGAT‑TTTCCCC‑‑GTTCCCGGTTGCt                                                                          >  2:2801959/1‑93 (MQ=255)

                  ccTTGTTAACCGCTGGCTTGTTAGGCACGCTGTTTGTGGTGATTAAAAAAAAATACTGTAACGCCTGACGAT‑TTTCCCC‑‑GTTCCCGGTTGCTg                                                                         >  1:4152542/1‑93 (MQ=255)

                       ttAACCGCTGGCTTGTTAGGCACGCTGTTTGTGGTGATTAAAAAAAAATACTGTAACGCCTGACGAT‑TTTCCCC‑‑GTTCCCGGTTGCTGTACCg                                                                    <  2:1306412/93‑1 (MQ=255)

                        tAACCGCTGGCTTGTTAGGCACGCTGTTTGTGGTGATTAAAAAAAAATACTGTAACGCCTGACGAT‑TTTCCCC‑‑GTTCCCGGTTGCTGTACCgg                                                                   >  1:4580227/1‑93 (MQ=255)

                          aCCGCTGGCTTGTTAGGCACGCTGTTTGTGGTGATTAAAAAAAAATACTGTAACGCCTGACGAT‑TTTCCCC‑‑GTTCCCGGTTGCTGTACCGGGa                                                                 >  1:1017940/1‑93 (MQ=255)

                           ccGCTGGCTTGTTAGGCACGCTGTTTGTGGTGATTAAAAAAAAATACTGTAACGCCTGACGAT‑TTTCCCC‑‑GTTCCCGGTTGCTGTACCGGGaa                                                                <  1:2930966/93‑1 (MQ=255)

                            cGCTGGCTTGTTAGGCACGCTGTTTGTGGTGATTAAAAAAAAATACTGTAACGCCTGACGAT‑TTTCCCC‑‑GTTCCCGGTTGCTGTACCGGGAAc                                                               >  1:1029515/1‑93 (MQ=255)

                            cGCTGGCTTGTTAGGCACGCTGTTTGTGGTGATTAAAAAAAAATACTGTAACGCCTGACGAT‑TTTCCCC‑‑GTTCCCGGTTGCTGTACCGGGAAc                                                               >  2:3679194/1‑93 (MQ=255)

                             gCTGGCTTGTTAGGCACGCTGTTTGTGGTGATTAAAAAAAAATACTGTAACGCCTGACGAT‑TTTCCCC‑‑GTTCCCGGTTGCTGTACCGGGAACg                                                              >  1:2998736/1‑93 (MQ=255)

                             gCTGGCTTGTTAGGCACGCTGTTTGTGGTGATTAAAAAAAAATACTGTAACGCCTGACGAT‑TTTCCCC‑‑GTTCCCGGTTGCTGTACCGGGAACg                                                              >  1:1086210/1‑93 (MQ=255)

                               tGGCTTGTTAGGCACGCTGTTTGTGGTGATTAAAAAAAAATACTGTAACGCCTGACGAT‑TTTCCCC‑‑GTTCCCGGTTGCTGTACCGGGCACGtt                                                            >  1:651691/1‑92 (MQ=255)

                               tGGCTTGTTAGGCACGCTGTTTGTGGTGATTAAAAAAAAATACTGTAACGCCTGACGAT‑TTTCCCC‑‑GTTCCCGGTTGCTGTACCGGGAACGTa                                                            >  2:4176495/1‑93 (MQ=255)

                                ggCTTGTTAGGCACGCTGTTTGTGGTGATTAAAAAAAAATACTGTAACGCCTGACGAT‑TTTCCCC‑‑GTTCCCGGTTGCTGTACCGGGAACGTAt                                                           >  1:356722/1‑93 (MQ=255)

                                ggCTTGTTAGGCACGCTGTTTGTGGTGATTAAAAAAAAATACTGTAACGCCTGACGAT‑TTTCCCC‑‑GTTCCCGGTTGCTGTACCGGGAACGTAt                                                           >  2:2888937/1‑93 (MQ=255)

                                ggCTTGTTAGGCACGCTGTTTGTGGTGATTAAAAAAAAATACTGTAACGCCTGACGAT‑TTTCCCC‑‑GTTCCCGGTTACTGTACCGGGAACGTAt                                                           >  1:4125847/1‑93 (MQ=255)

                                 gCTTGTTAGGCACGCTGTTTGTGGTGATTAAAAAAAAATACTGTAACGCCTGACGAT‑TTTCCCC‑‑GTTCCCGGTTGCTGTACCGGGAACGTAtt                                                          <  1:2209332/93‑1 (MQ=255)

                                 gCTTGTTAGGCACGCTGTTTGTGGTGATTAAAAAAAAATACTGTAACGCCTGACGAT‑TTTCCCC‑‑GTTCCCGGTTGCTGTACCGGGAACGTAtt                                                          <  1:670339/93‑1 (MQ=255)

                                 gCTTGTTAGGCACGCTGTTTGTGGTGATTAAAAAAAAATACTGTAACGCCTGACGAT‑TTTCCCC‑‑GTTCCCGGTTGCCGTACCGGGAACGTAtt                                                          >  2:4393187/1‑93 (MQ=255)

                                  cTTGTTAGGCACGCTGTTTGTGGTGATTAAAAAAAAATACTGTAACGCCTGACGAT‑TTTCCCC‑‑GTTCCCGGTTGCTGTACCGGGAACGTAttt                                                         >  1:199170/1‑93 (MQ=255)

                                    tGTTAGGCACGCTGTTTGTGGTGATTAAAAAAAAATACTGTAACGCCTGACGAT‑TTTCCCC‑‑GTTCCCGGTTGCTGTACCGGGAACGTATTTaa                                                       >  2:1200448/1‑93 (MQ=255)

                                      ttAGGCACGCTGTTTGTGGTGATTAAAAAAAAATACTGTAACGCCTGACGAT‑TTTCCCC‑‑GTTCCCGGTTGCTGTACCGGGAACGTATTTAAtt                                                     >  2:4554060/1‑93 (MQ=255)

                                         ggCACGCTGTTTGTGGTGATTAAAAAAAAATACTGTAACGCCTGACGAT‑TTTCCCC‑‑GTTCCCGGTTGCTGTACCGGGAACGTATTTAATTccc                                                  >  2:3665556/1‑93 (MQ=255)

                                         ggCACGCTGTTTGTGGTGATTAAAAAAAAATACTGTAACGCCTGACGAT‑TTTCCCC‑‑GTTCCCGGTTGCTGTACCGGGAACGTATTTAATTccc                                                  >  1:1527744/1‑93 (MQ=255)

                                          gCACGCTGTTTGTTGTGATTAAAAAAAAATACTGTAACGCCTGACGAT‑TTTCCCC‑‑GTTCCCGGTTGCTGTACCGGGAACGTATTTAATTcccc                                                 <  2:4599028/93‑1 (MQ=255)

                                              gCTGTTTGTGGTGATTAAAAAAAAATACTGTAACGCCTGACGAT‑TTTCCCC‑‑GTTCCCGGTTGCTGTACCGGGAACGTATTTAATTCCCCTGCa                                             >  2:298069/1‑93 (MQ=255)

                                               gtGTTTGTGGTTATTAAAAAAAAATACTGTAACGCCTGACGAT‑TTTCCCC‑‑GTTCCCGGTTGCTGTACCGGGAACGTATTTAATTCCCCTGCAt                                            <  1:180076/92‑1 (MQ=255)

                                               cTGTTTGTGGTGATTAAAAAAAAATACTGTAACGCCTGACGAT‑TTTCCCC‑‑GTTCCCGGTTGCTGTACCGGGAACGTATTTAATTCCCCTGCAt                                            <  2:337384/93‑1 (MQ=255)

                                                tGTTTGTGGTGATTAAAAAAAAATACTGTAACGCCTGACGAT‑TTTCCCC‑‑GTTCCCGGTTGCTGTACCGGGAACGTATTTAATTCCCCTGCATc                                           >  2:2211370/1‑93 (MQ=255)

                                                tGTTTGTGGTGATTAAAAAAAAATACTGTAACGCCTGACGAT‑TTTCCCC‑‑GTTCCCGGTTGCTGTACCGGGAACGTATTTAATTCCCCTGCATc                                           <  1:2620361/93‑1 (MQ=255)

                                                 gTTTGTGGTGATTAAAAAAAAATACTGTAACGCCTGACGAT‑TTTCCCC‑‑GTTCCCGGTTGCTGTACCGGGAACGTATTTAATTCCCCTGCATCg                                          >  1:2715036/1‑93 (MQ=255)

                                                     gtggtgATTAAAAAAAAATACTGTAACGCCTGACGAT‑TTTCCCC‑‑GTTCCCGGTTGCTGTACCGGGAACGTATTTAATTCCCCTGCATCGCCCg                                      >  2:1906178/1‑93 (MQ=255)

                                                     gtggtgATTAAAAAAAAATACTGTAACGCCTGACGAT‑TTTCCCC‑‑GTTCCCGGTTGCTGTACCGGGAACGTATTTAATTCCCCTGCATCGCCCg                                      >  2:3587641/1‑93 (MQ=255)

                                                      tggtgATTAAAAAAAAATACTGTAACGCCTGACGAT‑TTTCCCC‑‑GTTCCCGGTTGCTGTACCGGGAACGTATTTAATTCCCCTGCATCGCCCGc                                     >  1:490980/1‑93 (MQ=255)

                                                         tgATTAAAAAAAAATACTGTAACGCCTGACGAT‑TTTCCCC‑‑GTTCCCGGTTGCTGTACCGGGAACGTATTTAATTCCCCTGCATCGCCCGCAtt                                  >  1:2108532/1‑93 (MQ=255)

                                                         tgATTAAAAAAAAATACTGTAACGCCTGACGAT‑TTTCCCC‑‑GTTCCCGGTTGCTGTACCGGGAACGTATTTAATTCCCCTGCATCGCCCGCAtt                                  >  1:3393654/1‑93 (MQ=255)

                                                          gATTAAAAAAAAATACTGTAACGCCTGACGAT‑TTTCCCC‑‑GTTCCCGGTTGCTGTACCGGGAACGTATTTAATTCCCCTGCATCGCCCGCATTc                                 >  2:4615943/1‑93 (MQ=255)

                                                          gATTAAAAAAAAATACTGTAACGCCTGACGAT‑TTTCCCC‑‑GTTCCCGGTTGCTGTACCGGGAACGTATTTAATTCCCCTGCATCGCCCGCATTc                                 >  1:2667930/1‑93 (MQ=255)

                                                            ttAAAAAAAAATACTGTAACGCCTGACGAT‑TTTCCCC‑‑GTTCCCGGTTGCTGTACCGGGGACGCATTTAATTCCCCTGCATCGGCCGCATTCtt                               >  2:1835136/1‑93 (MQ=255)

                                                            ttAAAAAAAAATACTGTAACGCCTGACGAT‑TTTCCCC‑‑GTTCCCGGTTGCTGTACCGGGAACGTATTTAATTCCCCTGCATCGCCCGCATTCtt                               >  2:598059/1‑93 (MQ=255)

                                                            ttAAAAAAAAATACTGTAACGCCTGACGAT‑TTTCCCC‑‑GATCCCGGCTTCTTTAGCGGGGACCTATTTATATGTCCTGCCTCGCCCGCACTCtt                               >  2:1655435/1‑93 (MQ=255)

                                                             tAAAAAAAAATACTGTAACGCCTGACGAT‑TTTCCCC‑‑GTTCCCGGTTGCTGTACCGGGAACGTATTTAATTCTCCTGCATCGCCCGCATTCTTg                              >  2:506189/1‑93 (MQ=255)

                                                             tAAAAAAAAATACTGTAACGCCTGACGAT‑TTTCCCC‑‑GTTCCCGGTTGCTGTACCGGGAACGTATTTAATTCCCCTGCATCGCCCGCATTCTTg                              >  2:2729611/1‑93 (MQ=255)

                                                              aaaaaaaaaTACTGTAACGCCTGACGAT‑TTTCCCC‑‑GTTCCCGGTTGCTGTACCGGGAACGTATTTAATTCCCCTGCATCGCCCGCATTCTTGc                             <  1:3223347/93‑1 (MQ=255)

                                                              aaaaaaaaaTACTGTAACGCCTGACGAT‑TTTCCCC‑‑CTTCCCGGTTGCTGTACCGGGAACGCATTTAATTCCCCTGCACCGCCCGCATTCCTGc                             >  2:1106101/1‑93 (MQ=255)

                                                               aaaaaaaaTACTGTAACGCCTGACGAT‑TTTCCCC‑‑GTTCCCGGTTGCTGTACCGGGAACGTATTTAATTCCCCTGCATCGCCCGCATTCTTGcc                            >  2:3860136/1‑93 (MQ=255)

                                                               aaaaaaaaTACTGTAACGCCTGACGAT‑TTTCCCC‑‑GTTCCCGGTTGCTGTACCGGGAACGTATTTAATTCCCCTGCATCGCCCGCATTCTTGcc                            >  1:1192483/1‑93 (MQ=255)

                                                               aaaaaaaaTACTGTAACGCCTGACGAT‑TTTCCCC‑‑GTTCCCGGTTGCTGTACCGGGAACGTATTTAATTCCCCTGCATCGCCCGCATTCTTGcc                            <  2:2022169/93‑1 (MQ=255)

                                                               aaaaaaaaTACTGTAACGCCTGACGAT‑TTTCCCC‑‑GTTCCCGGTTGCTGTACCGGGAACGTATTTAATTCCCCTGCATCGCCCGCATTCTTGcc                            >  1:776672/1‑93 (MQ=255)

                                                               aaaaaaaaTACTGTAACGCCTGACGAT‑TTTCCCC‑‑GTTCCCGGTTGCTGTACCGGGAACGTATTTAATGCCCCTGCATCGCCCGCATTCTTGcc                            <  2:3325133/93‑1 (MQ=255)

                                                                aaaaaaaTACTGTAACGCCTGACGAT‑TTTCCCC‑‑GTTCCCGGTTGCTGTACCCGGGACGGATTTAATTTCCCTGCATCGCCCGCCTTCTTGccc                           >  2:821498/1‑92 (MQ=255)

                                                                 aaaaaaTACTGTAACGCCTGACGAT‑TTTCCCC‑‑GTTCCCGGTTGCTGTACCGGGAACGTATTTAATTCCCCTGCATCGCCCGCATTCTTGCCGc                          >  1:1109992/1‑93 (MQ=255)

                                                                 aaaaaaTACTGTAACGCCTGACGAT‑TTTCCCC‑‑GTTCCCGGTTGCTGTACCGGGAACGTATTTAATTCCCCTGCATCGCCCGCATTCTTGCCGc                          >  1:2754945/1‑93 (MQ=255)

                                                                    aaaTACTGTAACGCCTGACGAT‑TTTCCCC‑‑GTTCCCGGTTGCTGTACCGGGAACGTATTTAATTCCCCTGCATCGCCCGCATTCTTGCCGCATc                       <  2:1300777/93‑1 (MQ=255)

                                                                    aaaTACTGTAACGCCTGACGAT‑TTTCCCC‑‑GTTCCCGGTTGCTGTACCGGGAACGTATTTAATTCCCCTGCATCGCCCGCATTCTTGCCGCATc                       >  1:591535/1‑93 (MQ=255)

                                                                     aaTACTGTAACGCCTGACGAT‑TTTCCCC‑‑GTTCCCGGTTGCTGTACCGGGAACGTATTTAATTCCCCTGCATCGCCCGCATTCTTGCCGCATCt                      >  2:2286838/1‑93 (MQ=255)

                                                                     aaTACTGTAACGCCTGACGAT‑TTT‑CCCCGGTTCCCGG‑TGGTGTACCGGGGACGTATTTAATTCCCCCGCATCGCCCGCCATCTTTGCGCATCt                      >  2:3964524/1‑93 (MQ=255)

                                                                      aTACTGTAACGCCTGACGAT‑TTTCCCC‑‑GTTCCCGGTTGCTGTACCGGGAACGTATTTAATTCCCCTGCATCGCCCGCATTCTTGCCGCATCtt                     >  1:2593441/1‑93 (MQ=255)

                                                                       tACTGTAACGCCTGACGAT‑TTTCCCC‑‑GTTCCCGGTTGCTGTACCGGGAACGTATTTAATTCCCCTGCATCGCCCGCATTCTTGCCGCATCTTc                    >  1:1864246/1‑93 (MQ=255)

                                                                       tACTGTAACGCCTGACGAT‑TTTCCCC‑‑GTTCCCGGTTGCTGTACCGGGAACGTATTTAATTCCCCTGCATCGCCCGCATTCTTGCCGCATCTTc                    >  1:4235865/1‑93 (MQ=255)

                                                                        aCTGTAACGCCTGACGAT‑TTTCCCC‑‑GTTCCCGGTTGCTCTACCGGGGACGGATTTAATTCCCCTGCATCGCCCGCATTCCTTCCGCCTCtttc                   >  2:2533812/1‑91 (MQ=255)

                                                                          tGTAACGCCTGACGAT‑TTTCCCC‑‑GTTCCCGGTTGCTGTACCGGGAACGTATTTAATTCCCCTGCATCGCCCGCATTCTTGCCGCATCTTcccc                 <  2:4307012/93‑1 (MQ=255)

                                                                           gTAACGCCTGACGAT‑TTTCCCC‑‑GTTCCCGGTTGCTGTACCGGGAACGTATTTAATTCCCCTGCATCGCCCGCATTCTTGCCGCATCTTccccc                >  2:1465588/1‑93 (MQ=255)

                                                                            tAACGCCTGACGAT‑TTTCCCC‑‑GTTCCCGGTTGCTGTACCGGGAACGTATTTAATTCCCCTGCATCGCCCGCATTCTTGCCGCATCTTCCCCCg               >  2:792451/1‑93 (MQ=255)

                                                                             aaCGCCTGACGATGTAC‑CCC‑‑GTTCCCGGTTGCTGTACCGGGACTGTATTTAATTCCCCTGCATCGCCCGCATTCTTGCCGCGTCCTCCCCCgg              <  2:4572929/93‑1 (MQ=255)

                                                                              aCGCCTGACGAT‑TTTCCCC‑‑GTTCCCGGTTGCTGTACCGGGAACGTATTTAATTCCCCTGCATCGCCCGCATTCTTGCCGCATCTTCCCCCGGc             <  2:2396492/93‑1 (MQ=255)

                                                                               cGCCTGACGAT‑TTTCCCC‑‑GTTCCCGGTTGCTGTACCGGGAACGTATTTAATTCCCCTGCATCGCCCGCATTCTTGCCGCATCTTCCCCCGGCg            <  1:2978616/93‑1 (MQ=255)

                                                                                gCCTGACGAT‑TTTCCCC‑‑GTTCCCGGTTGCTGTACCGGGGACGTATTTAATTCCCCTGCATCGCCCGCATTCTTGCCGCATCTTCCCCCcgcgc           >  2:4076215/1‑92 (MQ=255)

                                                                                gCCTGACGAT‑TTTCCCC‑‑GTTCCCGGTTGCTGTACCGGGAACGTATTTAATTCCCCTGCATCGCCCGCATTCTTGCCGCATCTTCCCCCGGCGt           >  1:4346732/1‑93 (MQ=255)

                                                                                  cTGACGAT‑TTTCCCC‑‑GTTCCCCGGTGCTGTACCGGGGACGTATTTTATTTCCCTGCATCGCCCGCCTTCTTTCCGCATCTTCCCCCCGCGTcc         >  2:848660/1‑92 (MQ=255)

                                                                                   tGACGAT‑TTTCCCC‑‑GTTCCCGGTTGCTGTACCGGGAACGTATTTAATTCCCCTGCATCGCCCGCATTCTTGCCGCATCTTCCCCCGGCGTcac        >  2:141400/1‑93 (MQ=255)

                                                                                   tGACGAT‑TTTCCCC‑‑GTTCCCGGTTGCTGTACCGGGAACGTATTTAATTCCCCTGCATCGCCCGCATTCTTGCCGCATCTTCCCCCGGCGTcac        >  2:2785870/1‑93 (MQ=255)

                                                                                   tGACGAT‑TTTCCCC‑‑GTTCCCGGTTGCTGTACCGGGAACGTATTTAATTCCCCTGCATCGCCCGCATTCTTGCCGCATCTTCCCCCGGCGTcac        >  2:640548/1‑93 (MQ=255)

                                                                                   tGACGAT‑TTTCCCC‑‑GTTCCCGGTTGCTGTACCGGGAACGTATTTAATTCCCCTGCATCGCCCGCATTCTTGCCGCATCTTCCCCCGGCGTcac        <  2:3814349/93‑1 (MQ=255)

                                                                                   tGACGAT‑TTTCCCC‑‑GTTCCCGGTTGCTGTACCGGGAACGTATTTAATTCCCCTGCATCGCCCGCATTCTTGCCGCATCTTCCCCCCGCGTcac        >  1:1969375/1‑93 (MQ=255)

                                                                                   tGACGAT‑TTTCCCC‑‑GTTCCCGGTTGCTGTACCGGGAACGTATTTAATTCCCCTGCATCGCCCGCATTCTTGCCGCATCTTCCCCCCGCGTcac        >  1:1397459/1‑93 (MQ=255)

                                                                                    gACGAT‑TTTCCCC‑‑GTTCCCGGTTGCTGTACCGGGAACGTATTTAATTCCCCTGCATCGCCCGCATTCTTGCCGCATCTTCCCCCGGCGTcaca       <  1:1716957/93‑1 (MQ=255)

                                                                                     aCGAT‑TTTCCCC‑‑GTTCCCGGTTGCTGTACCGGGAACGTATTTAATTCCCCTGCATCGCCCGCATTCTTGCCGCATCTTCCCCCGGCGTCacac      >  1:3709861/1‑93 (MQ=255)

                                                                                      gatt‑ttc‑CCC‑‑GTTCCCGGTTGCTGTACCGGGAACGGATTTAATTCCCCTGCATCGCCCGCATTCTTGCCGCATCTTCCCCCGGCGTCACACCg    >  2:2835143/4‑93 (MQ=255)

                                                                                      cGAT‑TTTCCCC‑‑GTTCCCGGTTGCTGTACCGGGAACGTATTTAATTCCCCTGCATCGCCCGCATTCTTGCCGCATCTTCCCCCGGCGTCACAcc     <  1:1798213/93‑1 (MQ=255)

                                                                                      cGAT‑TTTCCCC‑‑GTTCCCGGTTGCTGTACCGGGAACGTATTTAATTCCCCTGCATCGCCCGCATTCTTGCCGCATCTTCCCCCGGCGTCACAcc     <  2:3214701/93‑1 (MQ=255)

                                                                                       att‑ttc‑CCC‑‑GTTCCCGGTTGCTGTACCGGGAACGTATTTAATTCCCCTGCATCGCCCGCATTCTTGCCGCATCTTCCCCCGGCGTCACACCGa   <  2:603828/91‑1 (MQ=255)

                                                                                       att‑ttc‑CCC‑‑GTTCCCGGTTGCTGTACCGGGAACGTATTTAATTCCCCTGCATCGCCCGCATTCTTGCCGCATCTTCCCCCGGCGTCACACCGa   <  1:1267806/91‑1 (MQ=255)

                                                                                        aT‑TTTCCCC‑‑GTTCCCCGTTGCTGTACCGGGGACGTATTTAATTCCCCTGCATCGCCCGCCTTCTTGCCGCATCTTCCCCCCGCGTCCCACCGa   >  2:4142358/1‑93 (MQ=255)

TGATGATCCTGCCAATTGCCTTGTTAACCGCTGGCTTGTTAGGCACGCTGTTTGTGGTGATTAAAAAAAAATACTGTAACGCCTGACGAT‑TTT‑CCC‑‑GTTCCCGGTTGCTGTACCGGGAACGTATTTAATTCCCCTGCATCGCCCGCATTCTTGCCGCATCTTCCCCCGGCGTCACACCGAA  >  NC_000913/3152152‑3152332

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 17 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

ATGATCCTGCCAATTGCCTTGTTAACCGCTGGCTTGTTAGGCACGCTGTTTGTGGTGATTAAAAAAAAATACTGTAACGCCTGACGATTTT‑CCC‑GTTCCCGGTTGCTGTACCGGGAACGTATTTAATTCCCCTGCAT‑CGCCCGCATTCTTGCCGCATCTTCCCCCGGCGTCACACCGAAGTAACGTT  >  NC_000913/3152154‑3152340

ATGATCCTGCCAATTGCCTTGTTAACCGCTGGCTTGTTAGGCACGCTGTTTGTGGTGATTAAAAAAAAATACTGTAACGCCTGACGATTTT‑CCC                                                                                                 >  SRR4302535.4408313/1‑94 (MQ=60)

ATGATCCTGCCAATTGCCTTGTTAACCGCTGGCTTGTTAGGCACGCTGTTTGTGGTGATTAAAAAAAAATACTGTAACGCCTGACGATTTT‑CCC                                                                                                 >  SRR4302535.4387331/1‑94 (MQ=60)

 TGATCCTGCCAATTGCCTTGTTAACCGCTGGCTTGTTAGGCACGCTGTTTGTGGTGATTAAAAAAAAATACTGTAACGCCTGACGATTTT‑CCC‑C                                                                                               >  SRR4302535.4432937/1‑94 (MQ=60)

 TGATCCTGCCAATTGCCTTGTTAACCGCTGGCTTGTTAGGCACGCTGTTTGTGGTGATTAAAAAAAAATACTGTAACGCCTGACGATTTT‑CCC‑C                                                                                               <  SRR4302535.3645804/94‑1 (MQ=60)

    TCCTGCCAATTGCCTTGTTAACCGCTGGCTTGTTAGGCACGCTGTTTGTGGTGATTAAAAAAAAATACTGTAACGCCTGACGATTTTCCCC‑GTT                                                                                             <  SRR4302535.1736268/94‑1 (MQ=60)

     CCTGCCAATTGCCTTGTTAACCGCTGGCTTGTTAGGCACGCTGTTTGTGGTGATTAAAAAAAAATACTGTAACGCCTGACGATTTTCCCC‑GTTC                                                                                            >  SRR4302535.2704008/1‑94 (MQ=60)

     CCTGCCAATTGCCTTGTTAACCGCTGGCTTGTTAGGCACGCTGTTTGTGGTGATTAAAAAAAAATACTGTAACGCCTGACGATTTTCCCC‑GTTC                                                                                            >  SRR4302535.4174589/1‑94 (MQ=60)

      CTGCCAATTGCCTTGTTAACCGCTGGCTTGTTAGGCACGCTGTTTGTGGTGATTAAAAAAAAATACTGTAACGCCTGACGATTTT‑CCCcgttgc                                                                                           >  SRR4302535.347809/1‑88 (MQ=60)

       TGCCAATTGCCTTGTTAACCGCTGGCTTGTTAGGCACGCTGTTTGTGGTGATTAAAAAAAAATACTGTAACGCCTGACGATTTTCCCC‑GTTCCC                                                                                          <  SRR4302535.893611/94‑1 (MQ=60)

       TGCCAATTGCCTTGTTAACCGCTGGCTTGTTAGGCACGCTGTTTGTGGTGATTAAAAAAAAATACTGTAACGCCTGACGATTTTCCCC‑GTTCCC                                                                                          >  SRR4302535.2789476/1‑94 (MQ=60)

         CCAATTGCCTTGTTAACCGCTGGCTTGTTAGGCACGCTGTTTGTGGTGATTAAAAAAAAATACTGTAACGCCTGACGATTTT‑CCCccttccccg                                                                                        >  SRR4302535.3223888/1‑85 (MQ=60)

           AATTGCCTTGTTAACCGCTGGCTTGTTAGGCACGCTGTTTGTGGTGATTAAAAAAAAATACTGTAACGCCTGACGATTTTCCCC‑GTTCCCGGTT                                                                                      >  SRR4302535.285774/1‑94 (MQ=60)

           AATTGCCTTGTTAACCGCTGGCTTGTTAGGCACGCTGTTTGTGGTGATTAAAAAAAAATACTGTAACGCCTGACGATTTTCCCC‑GTTCCCGGTT                                                                                      >  SRR4302535.2997251/1‑94 (MQ=60)

              TGCCTTGTTAACCGCTGGCTTGTTAGGCACGCTGTTTGTGGTGATTAAAAAAAAATACTGTAACGCCTGACGATTTTCCCC‑GTTCCCGGTTGCT                                                                                   <  SRR4302535.4163908/94‑1 (MQ=60)

               GCCTTGTTAACCGCTGGCTTGTTAGGCACGCTGTTTGTGGTGATTAAAAAAAAATACTGTAACGCCTGACGATTTTCCCC‑GTTCCCGGTTGCTG                                                                                  >  SRR4302535.4180975/1‑94 (MQ=60)

                 CTTGTTAACCGCTGGCTTGTTAGGCACGCTGTTTGTGGTGATTAAAAAAAAATACTGTAACGCCTGACGATTTTCCCC‑GTTCCCGGTTGCTGTA                                                                                >  SRR4302535.356549/1‑94 (MQ=60)

                   TGTTAACCGCTGGCTTGTTAGGCACGCTGTTTGTGGTGATTAAAAAAAAATACTGTAACGCCTGACGATTTTCCCC‑GTTCCCGGTTGCTGTACC                                                                              <  SRR4302535.1507222/94‑1 (MQ=60)

                   TGTTAACCGCTGGCTTGTTAGGCACGCTGTTTGTGGTGATTAAAAAAAAATACTGTAACGCCTGACGATTTTCCCC‑GTTCCCGGTTGCTGTACC                                                                              <  SRR4302535.3911157/94‑1 (MQ=60)

                   TGTTAACCGCTGGCTTGTTAGGCACGCTGTTTGTGGTGATTAAAAAAAAATACTGTAACGCCTGACGATTTT‑CCCcgcttcccggttctgcacc                                                                              >  SRR4302535.2478769/1‑75 (MQ=60)

                     TTAACCGCTGGCTTGTTAGGCACGCTGTTTGTGGTGATTAAAAAAAAATACTGTAACGCCTGACGATTTTCCCC‑GTTCCCGGTTGCTGTACCGG                                                                            >  SRR4302535.4611423/1‑94 (MQ=60)

                      TAGCCGCTGGCTTGTTAGGCACGCTTTTTGGGGTGATTAAAAAAAAATACTGTATCGCCTGACGATTTTCCCC‑TTTCCCGGTTGCTGTACCGGG                                                                           <  SRR4302535.2592214/94‑1 (MQ=60)

                       AACCGCTGGCTTGTTAGGCACGCTGTTTGTGGTGATTAAAAAAAAATACTGTAACGCCTGACGATTTTCCCC‑GTTCCCGGTTGCTGTACCGGGA                                                                          >  SRR4302535.1025175/1‑94 (MQ=60)

                        ACCGCTGGCTTGTTAGGCACGCTGTTTGTGGTGATTAAAAAAAAATACTGTAACGCCTGACGATTTTCCCC‑GTTCCCGGTTGCTGTACCGGGAA                                                                         <  SRR4302535.1264263/94‑1 (MQ=60)

                         CCGCTGGCTTGTTAGGCACGCTGTTTGTGGTGATTAAAAAAAAATACTGTAACGCCTGACGATTTTCCCC‑GTTCCCGGTTGCTGTACCGGGAAC                                                                        >  SRR4302535.1036822/1‑94 (MQ=60)

                         CCGCTGGCTTGTTAGGCACGCTGTTTGTGGTGATTAAAAAAAAATACTGTAACGCCTGACGATTTTCCCC‑GTTCCCGGTTGCTGTACCGGGAAC                                                                        <  SRR4302535.2951606/94‑1 (MQ=60)

                          CGCTGGCTTGTTAGGCACGCTGTTTGTGGTGATTAAAAAAAAATACTGTAACGCCTGACGATTTTCCCC‑GTTCCCGGTTGCTGTACCGGGAACG                                                                       >  SRR4302535.3019762/1‑94 (MQ=60)

                          CGCTGGCTTGTTAGGCACGCTGTTTGTGGTGATTAAAAAAAAATACTGTAACGCCTGACGATTTTCCCC‑GTTCCCGGTTGCTGTACCGGGAACG                                                                       >  SRR4302535.1093932/1‑94 (MQ=60)

                          CGCTGGCTTGTTAGGCACGCTGTTTGTGGTGATTAAAAAAAAATACTGTAACGCCTGACGATTTTCCCC‑GTTCCCGGTTGCTGTACCGGCAACG                                                                       >  SRR4302535.4243044/1‑94 (MQ=60)

                            CTGGCTTGTTAGGCACGCTGTTTGTGGTGATTAAAAAAAAATACTGTAACGCCTGACGATTTTCCCC‑GTTCCCGGTTGCTGTACCGGGCACGTT                                                                     >  SRR4302535.656238/1‑94 (MQ=60)

                            CTGGCTTGTTAGGCACGCTGTTTGTGGTGATTAAAAAAAAATACTGTAACGCCTGACGATTTTCCCC‑GTTCCCGGTTGCTGTACCGGGAACGTA                                                                     >  SRR4302535.1067901/1‑94 (MQ=60)

                            CTGGCTTGTTAGGCACGCTGTTTGTGGTGATTAAAAAAAAATACTGTAACGCCTGACGATTTTCCCC‑GTTCCCGGTTGCTGTACCGGGAACGTA                                                                     >  SRR4302535.2673753/1‑94 (MQ=60)

                             TGGCTTGTTAGGCACGCTGTTTGTGGTGATTAAAAAAAAATACTGTAACGCCTGACGATTTTCCCC‑GTTCCCGGTTGCTGTACCGGGAACGTAT                                                                    >  SRR4302535.359187/1‑94 (MQ=60)

                             TGGCTTGTTAGGCACGCTGTTTGTGGTGATTAAAAAAAAATACTGTAACGCCTGACGATTTTCCCC‑GTTCCCGGTTACTGTACCGGGAACGTAT                                                                    >  SRR4302535.4154083/1‑94 (MQ=60)

                               GCTTGTTAGGCACGCTGTTTGTGGTGATTAAAAAAAAATACTGTAACGCCTGACGATTTTCCCC‑GTTCCCGGTTGCTGTACCGGGAACGTATTT                                                                  <  SRR4302535.181259/94‑1 (MQ=60)

                               GCTTGTTAGGCACGCTGTTTGTGGTGATTAAAAAAAAATACTGTAACGCCTGACGATTTTCCCC‑GTTCCCGGTTGCTGTACCGGGAACGTATTT                                                                  <  SRR4302535.675018/94‑1 (MQ=60)

                               GCTTGTTAGGCACGCTGTTTGTGGTGATTAAAAAAAAATACTGTAACGCCTGACGATTTTCCCC‑GTTCCCGGTTGCTGTACCGGGAACGTATTT                                                                  <  SRR4302535.2225068/94‑1 (MQ=60)

                               GCTTGTTAGGCACGCTGTTTGTGGTGATTAAAAAAAAATACTGTAACGCCTGACGATTTTCCCC‑GTTCCCGGTTGCTGTACCGGGAACGTATTT                                                                  <  SRR4302535.3248463/94‑1 (MQ=60)

                               GCTTGTTAGGCACGCTGTTTGTGGTGATTAAAAAAAAATACTGTAACGCCTGACGATTTTCCCC‑GTTCCCGGTTGCTGTACCGGGAACGTATTT                                                                  >  SRR4302535.200535/1‑94 (MQ=60)

                                CTTGTTAGGCACGCTGTTTGTGGTGATTAAAAAAAAATACTGTAACGCCTGACGATTTTCCCC‑GTTCCCGGTTGCTGTACCGGGAACGTATTTA                                                                 <  SRR4302535.3643050/94‑1 (MQ=60)

                                CTTGTTAGGCACGCTGTTTGTGGTGATTAAAAAAAAATACTGTAACGCCTGACGATTTTCCCC‑GTTCCCGGGTGCTGTACCGGGGACGTATTTA                                                                 >  SRR4302535.884550/1‑94 (MQ=60)

                                 TTGTTAGGCACGCTGTTTGTGGTGATTAAAAAAAAATACTGTAACGCCTGACGATTTTCCCC‑GTTCCCGGTTGCTGTACCGGGAACGTATTTAA                                                                <  SRR4302535.1545925/94‑1 (MQ=60)

                                 TTGTTAGGCACGCTGTTTGTGGTGATTAAAAAAAAATACTGTAACGCCTGACGATTTTCCCC‑GTTCCCGGTTGCTGTACCGGGAACGTATTTAA                                                                >  SRR4302535.669890/1‑94 (MQ=60)

                                  TGTTAGGCACGCTGTTTGTGGTGATTAAAAAAAAATACTGTAACGCCTGACGATTTTCCCC‑GTTCCCGGTTGCTGTACCGGGAACGTATTTAAT                                                               <  SRR4302535.4660314/94‑1 (MQ=60)

                                    TTAGGCACGCTGTTTGTGGTGATTAAAAAAAAATACTGTAACGCCTGACGATTTTCCCC‑GTTCCCGGTTGCTGTACCGGGAACGTATTTAATTC                                                             <  SRR4302535.1857655/94‑1 (MQ=60)

                                      AGGCACGCTGTTTGTGGTGATTAAAAAAAAATACTGTAACGCCTGACGATTTTCCCC‑GTTCCCGGTTGCTGTACCGGGGACGTATTTAATTCCC                                                           >  SRR4302535.4317456/1‑94 (MQ=60)

                                      AGGCACGCTGTTTGTGGTGATTAAAAAAAAATACTGTAACGCCTGACGATTTTCCCC‑GTTCCCGGTTGCTGTACCGGGAACGTATTTAATTCCC                                                           >  SRR4302535.1538749/1‑94 (MQ=60)

                                        GCCCGCTGTTTGTGGTGATTAAAAAAAAATACTGTAACGCCTGACGATTTTCCCC‑GTTCCCGGTTGCTGTACCGGGAACGTATTTAATTCCCCT                                                         <  SRR4302535.1101334/94‑1 (MQ=60)

                                        GCACGCTGTTTGTGGTGATTAAAAAAAAATACTGTAACGCCTGACGATTTTCCCC‑GTTCCCGGTTGCTGTACCGGGAACGTATTTAATTCCCCT                                                         <  SRR4302535.2614597/94‑1 (MQ=60)

                                        GCACGCTGTTTGTGGTGATTAAAAAAAAATACTGTAACGCCTGACGATTTTCCCC‑GTTCCCGGTTGCTGTACCGGGAACGTATTTAATTCCCCT                                                         <  SRR4302535.1280336/94‑1 (MQ=60)

                                         CACGCTGTTTGTGGTGATTAAAAAAAAATACTGTAACGCCTGACGATTTTCCCC‑GTTCCCGGTTGCTGTACCGGGAACGTATTTAATTCCCCTA                                                        >  SRR4302535.2013843/1‑94 (MQ=60)

                                         CACGCTGTTTGTGGTGATTAAAAAAAAATACTGTAACGCCTGACGATTTT‑CCCAGATCCCGGTTGCTGTACCGGGAACGgctttaattcaccag                                                        >  SRR4302535.259027/1‑79 (MQ=60)

                                            GCTGTTTGTGGTGATTAAAAAAAAATACTGTAACGCCTGACGATTTTCCCC‑GTTCCCGGTTGCTGTACCGGGAACGTATTTAATTCCCCTGCAT                                                     <  SRR4302535.3684290/94‑1 (MQ=60)

                                             GTGTTTGTGGTTATTAAAAAAAAATACTGTAACGCCTGACGATTTTCCCC‑GTTCCCGGTTGCTGTACCGGGAACGTATTTAATTCCCCTGCAT‑C                                                   <  SRR4302535.181327/94‑1 (MQ=60)

                                             CTGTTTGTGGTGATTAAAAAAAAATACTGTAACGCCTGACGATTTTCCCC‑GTTCCCGGTTGCTGTACCGGGAACGTATTTAATTCCCCTGCAT‑C                                                   >  SRR4302535.1970764/1‑94 (MQ=60)

                                              TGTTTGTGGTGATTAAAAAAAAATACTGTAACGCCTGACGATTTTCCCC‑GTTCCCGGTTGCTGTACCGGGAACGTATTTAATTCCCCTGCAT‑CG                                                  >  SRR4302535.2734432/1‑94 (MQ=60)

                                              TGTTTGTGGTGATTAAAAAAAAATACTGTAACGCCTGACGATTTTCCCC‑GTTCCCGGTTGCTGTACCGGGAACGTATTTAATTCCCCTGCAT‑CG                                                  <  SRR4302535.2639145/94‑1 (MQ=60)

                                               GTTTGTGGTGATTAAAAAAAAATACTGTAACGCCTGACGATTTTCCCC‑GTTCCCGGTTGCTGTACCGGGAACGTATTTAATTCCCCTGCAT‑CGC                                                 >  SRR4302535.1157866/1‑94 (MQ=60)

                                                   GTGGTGATTAAAAAAAAATACTGTAACGCCTGACGATTTTCCCC‑GTTCCCGGTTGCTGTACCGGGAACGTATTTAATTCCCCTGCAT‑CGCCCGC                                             >  SRR4302535.494423/1‑94 (MQ=60)

                                                      GTGATTAAAAAAAAATACTGTAACGCCTGACGATTTTCCCC‑GTTCCCGGTTGCTGTACCGGGAACGTATTTAATTCCCCTGCAT‑CGCCCGCATT                                          >  SRR4302535.4386398/1‑94 (MQ=60)

                                                      GTGATTAAAAAAAAATACTGTAACGCCTGACGATTTTCCCC‑GTTCCCGGTTGCTGTACCGGGAACGTATTTAATTCCCCTGCAT‑CGCCCGCATT                                          >  SRR4302535.2123738/1‑94 (MQ=60)

                                                      GTGATTAAAAAAAAATACTGTAACGCCTGACGATTTTCCCC‑GTTCCCGGTTGCTGTACCGGGAACGTATTTAATTCCCCTGCAT‑CGCCCGCATT                                          >  SRR4302535.3417265/1‑94 (MQ=60)

                                                       TGATTAAAAAAAAATACTGTAACGCCTGACGATTTTCCCC‑GTTCCCGGTTGCTGTACCGGGAACGTATTTAATTCCCCTGCAT‑CGCCCGCATTC                                         >  SRR4302535.2687026/1‑94 (MQ=60)

                                                          TTAAAAAAAAATACTGTAACGCCTGACGATTTTCCCC‑GTTCCCGGTTGCTGTACCGGGAACGTATTTAATTCCCCTGCAT‑CGCCCGCATTCTTG                                      <  SRR4302535.3008482/94‑1 (MQ=60)

                                                            AAAAAAAAATACTGTAACGCCTGACGATTTTCCCC‑GTTCCCGGTTGCTGTACCGGGAACGTATTTAATTCCCCTGCAT‑CGCCCGCATTCTTGCC                                    <  SRR4302535.3245690/94‑1 (MQ=60)

                                                            AAAAAAAAATACTGTAACGCCTGACGATTTTCCCC‑GTTCCCGGTTGCTGTACCGGGAACGTATTTAATTCCCCTGCAT‑CGCCCGCATTCTTGCC                                    >  SRR4302535.1201023/1‑94 (MQ=60)

                                                            AAAAAAAAATACTGTAACGCCTGACGATTTTCCCC‑GTTCCCGGTTGCTGTACCGGGAACGTATTTAATTCCCCTGCAT‑CGCCCGCATTCTTGCC                                    >  SRR4302535.782129/1‑94 (MQ=60)

                                                             AAAAAAAATACTGTAACGCCTGACGATTTTCCCC‑GTTCCCGGTTGCTGTACCGGGAACGTATTTAATTCCCCTGCAT‑CGCCCGCATTCTTGCCG                                   >  SRR4302535.4062179/1‑94 (MQ=60)

                                                              AAAAAAATACTGTAACGCCTGACGATTTTCCCC‑GTTCCCGGTTGCTGTACCGGGAACGTATTTAATTCCCCTGCAT‑CGCCCGCATTCTTGCCGC                                  >  SRR4302535.1117920/1‑94 (MQ=60)

                                                              AAAAAAATACTGTAACGCCTGACGATTTTCCCC‑GTTCCCGGTTGCTGTACCGGGAACGTATTTAATTCCCCTGCAT‑CGCCCGCATTCTTGCCGC                                  >  SRR4302535.2774584/1‑94 (MQ=60)

                                                              AAAAAAATACTGTAACGCCTGACGATTTTCCCC‑GTTCCCGGTTGCTGTACCGGGAACGTATTTAATTCCCCTGCAT‑CGCCCGCATTCTTGCCGC                                  >  SRR4302535.2747600/1‑94 (MQ=60)

                                                               AAAAAATACTGTAACGCCTGACGATTTTCCCC‑GTTCCCGGTTGCTGTACCGGGAACGTATTTAATTCCCCTGCAT‑CGCCCGCATTCTTGCCGCA                                 >  SRR4302535.3185424/1‑94 (MQ=60)

                                                                AAAAATACTGTAACGCCTGACGATTTTCCCC‑GTTCCCGGTTGCTGTACCGGGAACGTATTTAATTCCCCTGCAT‑CGCCCGCATTCTTGCCGCAT                                <  SRR4302535.3782187/94‑1 (MQ=60)

                                                                 AAAATACTGTAACGCCTGACGATTTTCCCC‑GTTCCCGGTTGCTGTACCGGGAACGTATTTAATTCCCCTGCAT‑CGCCCGCATTCTTGCCGCATC                               >  SRR4302535.595680/1‑94 (MQ=60)

                                                                  AAATACTGTAACGCCTGACGATTTTCCCC‑GTTCCCGGTTGCTGTACCGGGAACGTATTTAATTCCCCTGCAT‑CGCCCGCATTCTTGCCGCATCT                              <  SRR4302535.2937754/94‑1 (MQ=60)

                                                                  AAATACTGTAACGCCTGACGATTTTCCCC‑GTTCCCGGTTGCTGTACCGGGAACGTATTTAATTCCCCTGCAT‑CGCCCGCATTCTTGCCGCATCT                              <  SRR4302535.4443506/94‑1 (MQ=60)

                                                                   AATACTGTAACGCCTGACGATTTTCCCC‑GTTCCCGGTTGCTGTACCGGGAACGTATTTAATTCCCCTGCAT‑CGCCCGCATTCTTGCCGCATCTT                             >  SRR4302535.2612071/1‑94 (MQ=60)

                                                                    ATACTGTAACGCCTGACGATTTTCCCC‑GTTCCCGGTTGCTGTACCGGGAACGTATTTAATTCCCCTGCAT‑CGCCCGCATTCTTGCCGCATCTTC                            >  SRR4302535.1877690/1‑94 (MQ=60)

                                                                    ATACTGTAACGCCTGACGATTTTCCCC‑GTTCCCGGTTGCTGTACCGGGAACGTATTTAATTCCCCTGCAT‑CGCCCGCATTCTTGCCGCATCTTC                            >  SRR4302535.4264855/1‑94 (MQ=60)

                                                                      ACTGTAACGCCTGACGATTTTCCCC‑GTTCCCGGCTGCTGTACCCGGGACGTATTTCATTCCCCCGCCTCCGCCCCCA‑TCTTGCCGCATCTTCCC                          >  SRR4302535.916163/1‑94 (MQ=26)

                                                                          TAACGCCTGACGATTTTCCCC‑GTTCCCGGTTGCTGTACCGGGAACGTATTTAATTCCCCTGCAT‑CGCCCGCATTCTTGCCGCATCTTCCCCCGG                      >  SRR4302535.614263/1‑94 (MQ=60)

                                                                             CGCCTGACGATTTTCCCC‑GTTCCCGGTTGCTGTACCGGGAACGTATTTAATTCCCCTGCAT‑CGCCCGCATTCTTGCCGCATCTTCCCCCGGCGT                   >  SRR4302535.4376472/1‑94 (MQ=60)

                                                                             CGCCTGACGATTTTCCCC‑GTTCCCGGTTGCTGTACCGGGAACGTATTTAATTCCCCTGCAT‑CGCCCGCATTCTTGCCGCATCTTCCCCCGGCGT                   <  SRR4302535.2999515/94‑1 (MQ=60)

                                                                                CTGACGATTTTCCCC‑GTTCCCGGTTGCTGTACCGGGAACGTATTTAATTCCCCTGCAT‑CGCCCGCATTCTTGCCGCATCTTCCCCCGGCGTCAC                >  SRR4302535.2000196/1‑94 (MQ=60)

                                                                                CTGACGATTTTCCCC‑GTTCCCGGTTGCTGTACCGGGAACGTATTTAATTCCCCTGCAT‑CGCCCGCATTCTTGCCGCATCTTCCCCCCGCGTCAC                >  SRR4302535.1407435/1‑94 (MQ=60)

                                                                                CTGACGATTTTCCCC‑GTTCCCGGTTGCTGTACCGGGAACGTATTTAATTCCCCTGCAT‑CGCCCGCATTCTTGCCGCATCTTCCCCCCGCGTCAC                >  SRR4302535.1983563/1‑94 (MQ=60)

                                                                                CTGACGATTTTCCCC‑GTTCCCGGTTGCTGTACCGGGAACGGATTTAATTCCCCTGGAT‑CGCCCCCATTCTTGCCGCATCTTCCCCCGGCGTCAC                >  SRR4302535.909522/1‑94 (MQ=60)

                                                                                  GACGATTTTCCCC‑GTTCCCGGTTGCTGTACCGGGAACGTATTTAATTCCCCTGCAT‑CGCCCGCATTCTTGCCGCATCTTCCCCCGGCGTCACAC              >  SRR4302535.3735554/1‑94 (MQ=60)

                                                                                  GACGATTTTCCCC‑GTTCCCGGTTGCTGTACCGGGAACGTATTTAATTCCCCTGCAT‑CGCCCGCATTCTTGCCGCATCTTCCCCCGGCGTCACAC              <  SRR4302535.1729379/94‑1 (MQ=60)

                                                                                    CGATTTTCCCC‑GTTCCCGGTTGCTGTACCGGGAACGTATTTAATTCCCCTGCAT‑CGCCCGCATTCTTGCCGCATCTTCCCCCGGCGTCACACCG            <  SRR4302535.1811215/94‑1 (MQ=60)

                                                                                    CGATTTTCCCC‑GTTCCCGGTTGCTGTACCGGGAACGTATTTAATTCCCCTGCAT‑CGCCCGCATTCTTGCCGCATCTTCCCCCGGCGTCACACCG            >  SRR4302535.2144685/1‑94 (MQ=60)

                                                                                     GATTTTCCCC‑GTTCCCGGTTGCTGTACCGGGAACGTATTTAATTCCCCTGCAT‑CGCCCGCATTCTTGCCGCATCTTCCCCCGGCGTCACACCGA           >  SRR4302535.677971/1‑94 (MQ=60)

                                                                                      ATTTTCCCC‑GTTCCCGGTTGCTGTACCGGGAACGTATTTAATTCCCCTGCAT‑CGCCCGCATTCTTGCCGCATCTTCCCCCGGCGTCACACCGAA          <  SRR4302535.1276877/94‑1 (MQ=60)

                                                                                        TTC‑CCC‑GTTCCCGGTTGCTGTACCGGGAACGTATTTAATTCCCCTGCAT‑CGCCCGCATTCTTGCCGCATCTTCCCCCGGCGTCACACCGAAGTA       <  SRR4302535.3189910/94‑1 (MQ=60)

                                                                                          C‑CCC‑GTTCCCGGTTGCTGTACCGGGAACGTATTTAATTCCCCTGCAT‑CGCCCGCATTCTTGCCGCATCTTCCCCCGGCGTCACACCGAAGTAAC     <  SRR4302535.4176061/94‑1 (MQ=60)

                                                                                          C‑CCC‑GTTCCCGGTTGCTGTACCGGGAACGTATTTAATTCCCCTGCAT‑CGCCCGCATTCTTGCCGCATCTTCCCCCGGCGTCACACCGAAGTAAC     <  SRR4302535.2544528/94‑1 (MQ=60)

                                                                                            CCC‑GTTCCCGGTTGCTGTACCGGGAACGTATTTAATTCCCCTGCAT‑CGCCCGCATCCTTGCCGCATCTTCCCCCGGCGTCACACCGAAGTAACG    <  SRR4302535.4667142/94‑1 (MQ=60)

                                                                                            CCC‑CTTCCCGGTTGCTGTACCGGCAACGTATTTAATCCCCCTGCAT‑CGCCCGCATTCTTGCCGCATCTTCCCCCGGCGTCACACCGAAGTAACG    <  SRR4302535.1541561/94‑1 (MQ=60)

                                                                                             CC‑GTTCCCGGTTGCTGTACCGGGAACGTATTTAATTCCCCTGCAT‑CGCCCGCATTCTTGCCGCATCTTCCCCCGGCGTCACACCGAAGTAACGT   <  SRR4302535.3513224/94‑1 (MQ=60)

                                                                                             CC‑GTTCCCGGTTGCTGTACCGGGAACGTATTTAATTCCCCTGCAT‑CGCCCGCATTCTTGCCGCATCTTCCCCCGGCGTCACACCGAAGTAACGT   >  SRR4302535.3167359/1‑94 (MQ=60)

                                                                                              C‑GTTCCCGGTTGCTGTACCGGGAACGTATTTAATTCCCCTGCAT‑CGCCCGCATTCTTGCCGCATCTTCCCCCGGCGTCACACCGCAGTAACGTT  >  SRR4302535.679374/1‑94 (MQ=60)

                                                                                              C‑GTTCCCGGTTGCTGTACCGGGAACGTATTTAATTCCCCTGCAT‑CGCCCGCATTCTTGCCGCATCTTCCCCCGGCGTCACACCGAAGTAACGTT  <  SRR4302535.312881/94‑1 (MQ=60)

                                                                                              C‑GTTCCCGGTTGCTGTACCGGGAACGTATTTAATTCCCCTGCAT‑CGCCCGCATTCTTGCCGCATCTTCCCCCGGCGTCACACCGAAGTAACGTT  >  SRR4302535.2762499/1‑94 (MQ=60)

ATGATCCTGCCAATTGCCTTGTTAACCGCTGGCTTGTTAGGCACGCTGTTTGTGGTGATTAAAAAAAAATACTGTAACGCCTGACGATTTT‑CCC‑GTTCCCGGTTGCTGTACCGGGAACGTATTTAATTCCCCTGCAT‑CGCCCGCATTCTTGCCGCATCTTCCCCCGGCGTCACACCGAAGTAACGTT  >  NC_000913/3152154‑3152340

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 2 ≤` `ATCG/ATCG` `< 23 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`