Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 1873 183.1 9376148 98.2% 9207377 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,188,237 T→C V114A (GTG→GCG)  b3047 → putative membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,188,2370TC99.6% 824.9 / ‑5.1 263V114A (GTG→GCG) b3047putative membrane protein
Reads supporting (aligned to +/- strand):  ref base T (1/0);  new base C (139/123);  total (140/123)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.02e-01

Only 100 of 276 total aligned reads displayed.

ATCCCAGTGAGCAAATTCAAATAAGAATGATAACTCAGGAAAAAATTGCTCAACTTCCTAAAGACAGAGAAACGCTCTTCTATTTTAACGTGCGAGAAATACCGCCCAAAACAGATAAAAAAAATGTGATGCAAGTCACTATGCAGCACGCATTGAAACTATTCTGGCGGCCAAAAGCCA  >  NC_000913/3188147‑3188326
                                                                                          |                                                                                         
aTCCCAGTGAGCAAATTCAAATAAGAATGATAACTCAGGAAAAAATTGCTCAACTTCCTAAAGACAGAGAAACGCTCTTCTATTTTAACgcgc                                                                                         <  1:3883746/93‑1 (MQ=255)
aTCCCAGTGAGCAAATTCAAATAAGAATGATAACTCAGGAAAAAATTGCTCAACTTCCTAAAGACAGAGAAACGCTCTTCTATTTTAACgcgc                                                                                         >  2:4359748/1‑93 (MQ=255)
aTCCCAGTGAGCAAATTCAAATAAGAATGATAACTCAGGAAAAAATTGCTCAACTTCCTAAAGACAGAGAAACGCTCTTCTATTTTAACgcgc                                                                                         >  1:1927040/1‑93 (MQ=255)
 tCCCAGTGAGCAAATTCAAATAAGAATGATAACTCAGGAAAAAATTGCTCAACTTCCTAAAGACAGAGAAACGCTCTTCTATTTTAAcgcgcg                                                                                        >  1:2960877/1‑93 (MQ=255)
  cccAGTGAGCAAATTCAAATAAGAATGATAACTCAGGAAAAAATTGCTCAACTTCCTAAAGACAGAGAAACGCTCTTCTATTTTAACGCGCga                                                                                       <  2:4131286/93‑1 (MQ=255)
   ccAGTGAGCAAATTCAAATAAGAATGATAACTCAGGAAAAAATTGCTCAACTTCCTAAAGACAGAGAAACGCTCTTCTATTTTAACGCGCgag                                                                                      >  1:3329299/1‑93 (MQ=255)
     aGTGAGCAAATTCAAATAAGAATGATAACTCAGGAAAAAATTGCTCAACTTCCTAAAGACAGAGAAACGCTCTTCTATTTTAACGCGCGAGaa                                                                                    >  2:318532/1‑93 (MQ=255)
      gTGAGCAAATTCAAATAAGAATGATAACTCAGGAAAAAATTGCTCAACTTCCTAAAGACAGAGAAACGCTCTTCTATTTTAACGCGCGAGaaa                                                                                   <  1:3411096/93‑1 (MQ=255)
        gAGCAAATTCAAATAAGAATGATAACTCAGGAAAAAATTGCTCAACTTCCTAAAGACAGAGAAACGCTCTTCTATTTTAACGCGCGAGAAATa                                                                                 <  2:4662932/93‑1 (MQ=255)
        gAGCAAATTCAAATAAGAATGATAACTCAGGAAAAAATTGCTCAACTTCCTAAAGACAGAGAAACGCTCTTCTATTTTAACGCGCGAGAAATa                                                                                 <  1:3777704/93‑1 (MQ=255)
        gAGCAAATTCAAATAAGAATGATAACTCAGGAAAAAATTGCTCAACTTCCTAAAGACAGAGAAACGCTCTTCTATTTTAACGCGCGAGAAATa                                                                                 >  1:9273/1‑93 (MQ=255)
         aGCAAATTCAAATAAGAATGATAACTCAGGAAAAAATTGCTCAACTTCCTAAAGACAGAGAAACGCTCTTCTATTTTAACGCGCGAGAAATAc                                                                                <  1:3704445/93‑1 (MQ=255)
         aGCAAATTCAAATAAGAATGATAACTCAGGAAAAAATTGCTCAACTTCCTAAAGACAGAGAAACGCTCTTCTATTTTAACGCGCGAGAAATAc                                                                                <  2:2430474/93‑1 (MQ=255)
         aGCAAATTCAAATAAGAATGATAACTCAGGAAAAAATTGCTCAACTTCCTAAAGACAGAGAAACGCTCTTCTATTTTAACGCGCGAGAAATAc                                                                                <  2:3128025/93‑1 (MQ=255)
          gCAAATTCAAATAAGAATGATAACTCAGGAAAAAATTGCTCAACTTCCTAAAGACAGAGAATCGCTCTTCTATTTTAACGCGCGAGAAATAcc                                                                               <  2:2045774/93‑1 (MQ=255)
          gCAAATTCAAATAAGAATGATAACTCAGGAAAAAATTGCTCAACTTCCTAAAGACAGAGAAACGCTCTTCTATTTTAACGCGCGAGAAATAcc                                                                               >  1:1665644/1‑93 (MQ=255)
          gCAAATTCAAATAAGAATGATAACTCAGGAAAAAATTGCTCAACTTCCTAAAGACAGAGAAACGCTCTTCTATTTTAACGCGCGAGAAATAcc                                                                               <  2:3380474/93‑1 (MQ=255)
            aaaTTCAAATAAGAATGATAACTCAGGAAAAAATTGCTCAACTTCCTAAAGACAGAGAAACGCTCTTCTATTTTAACGCGCGAGAAATACCGc                                                                             >  2:3093291/1‑93 (MQ=255)
             aaTTCAAATAAGAATGATAACTCAGGAAAAAATTGCTCAACTTCCTAAAGACAGAGAAACGCTCTTCTATTTTAACGCGCGAGAAATACCGcc                                                                            >  2:2222523/1‑93 (MQ=255)
               ttCAAATAAGAATGATAACTCAGGAAAAAATTGCTCAACTTCCTAAAGACAGAGAAACGCTCTTCTATTTTAACGCGCGAGAAATACCGCCCa                                                                          >  2:2574363/1‑93 (MQ=255)
               ttCAAATAAGAATGATAACTCAGGAAAAAATTGCTCAACTTCCTAAAGACAGAGAAACGCTCTTCTATTTTAACGCGCGAGAAATACCGCCCa                                                                          >  2:1279466/1‑93 (MQ=255)
               atCAAATAAGAATGATAACTCAGGAAAAAATTGCTCAACTTCCTAAAGACAGAAAAACCCTCTTCTATTTTAACGCGCGAGAAATTCCGCCCa                                                                          <  2:894045/92‑1 (MQ=255)
                 cAAATAAGAATGATAACTCAGGAAAAAATTGCTCAACTTCCTAAAGACAGAGAAACGCTCTTCTATTTTAACGCGCGAGAAATACCGCCCaaa                                                                        >  1:1800899/1‑93 (MQ=255)
                 cAAATAAGAATGATAACTCAGGAAAAAATTGCTCAACTTCCTAAAGACAGAGAAACGCTCTTCTATTTTAACGCGCGAGAAATACCGCCCaaa                                                                        >  1:4203675/1‑93 (MQ=255)
                  aaaTAAGAATGATAACTCAGGAAAAAATTGCTCAACTTCCTAAAGACAGAGAAACGCTCTTCTATTTTAACGCGCGAGAAATACCGCCCaaaa                                                                       <  2:3308169/93‑1 (MQ=255)
                  aaaTAAGAATGATAACTCAGGAAAAAATTGCTCAACTTCCTAAAGACAGAGAAACGCTCTTCTATTTTAACGCGCGAGAAATACCGCCCaaaa                                                                       >  2:1677352/1‑93 (MQ=255)
                  aaaTAAGAATGATAACTCAGGAAAAAATTGCTCAACTTCCTAAAGACAGAGAAACGCTCTTCTATTTTAACGCGCGAGAAATACCGCCCaaaa                                                                       >  1:4451811/1‑93 (MQ=255)
                  aaaTAAGAATGATAACTCAGGAAAAAATTGCTCAACTTCCTAAAGACAGAGAAACGCTCTTCTATTTTAACGCGCGAGAAATACCGCCCaaaa                                                                       <  1:1707727/93‑1 (MQ=255)
                    aTAAGAATGATAACTCAGGAAAAAA‑TGCTCAAATTCCTAAAGACAGAGAAAAGCTCTTCTATTTTAACGCGCGAGAAATACCGCCCAAAACaa                                                                    >  1:534786/1‑92 (MQ=255)
                     tAAGAATGATAACTCAGGAAAAAATTGCTCAACTTCCTAAAGACAGAGAAACGCTCTTCTATTTTAACGCGCGAGAAATACCGCCCAAAACAg                                                                    <  1:3738262/93‑1 (MQ=255)
                     tAAGAATGATAACTCAGGAAAAAATTGCTCAACTTCCTAAAGACAGAGAAACGCTCTTCTATTTTAACGCGCGAGAAATACCGCCCAAAACAg                                                                    >  2:475140/1‑93 (MQ=255)
                       aGAATGATAACTCAGGAAAAAATTGCTCAACTTCCTAAAGACAGAGAAACGCTCTTCTATTTTAACGCGCGAGAAATACCGCCCAAAACAGAt                                                                  <  1:836167/93‑1 (MQ=255)
                       aGAATGATAACTCAGGAAAAAATTGCTCAACTTCCTAAAGACAGAGAAACGCTCTTCTATTTTAACGCGCGAGAAATACCGCCCAAAACAGAt                                                                  <  2:2136517/93‑1 (MQ=255)
                       aGAATGATAACTCAGGAAAAAATTGCTCAACTTCCTAAAGACAGAGAAACGCTCTTCTATTTTAACGCGCGAGAAATACCGCCCAAAACAGAt                                                                  >  2:3754377/1‑93 (MQ=255)
                       aGAATGATAACTCAGGAAAAAATTGCTCAACTTCCTAAAGACAGAGAAACGCTCTTCTATTTTAACGCGCGAGAAATACCGCCCAAAACAGAt                                                                  <  2:3584113/93‑1 (MQ=255)
                        gAATGATAACTCAGGAAAAAATTGCTCAACTTCCTAAAGACAGAGAAACGCTCTTCTATTTTAACGCGCGAGAAATACCGCCCAAAACAGATa                                                                 <  1:4593852/93‑1 (MQ=255)
                         aaTGATAACTCAGGAAAAAATTGCTCAACTTCCTAAAGACAGAGAAACGCTCTTCTATTTTAACGCGCGAGAAATACCGCCCAAAACAGATaa                                                                >  1:2570690/1‑93 (MQ=255)
                           tGATAACTCAGGAAAAAAGTGCTCAACTTCCTAAAGACAGAGAAACGCTCTTCTATTTTAACGCGCGAGAAATACCGCCCAAAACAGATaaaa                                                              <  1:1284169/93‑1 (MQ=255)
                            gATAACTCAGGAAAAAATTGCTCAACTTCCTAAAGACAGAGAAACGCTCTTCTATTTTAACGCGCGAGAAATACCGCCCAAAACAGATaaaaa                                                             <  1:4164068/93‑1 (MQ=255)
                              tAACTCAGGAAAAAATTGCTCAACTTCCTAAAGACAGAGAAACGCTCTTCTATTTTAACGCGCGAGAAATACCGCCCAAAACAGATaaaaaaa                                                           >  1:2420989/1‑93 (MQ=255)
                              tAACTCAGGAAAAAATTGCTCAACTTCCTAAAGACAGAGAAACGCTCTTCTATTTTAACGCGCGAGAAATACCGCCCAAAACAGATaaaaaaa                                                           >  2:620206/1‑93 (MQ=255)
                                aCTCAGGAAAAAATTGCTCAACTTCCTAAAGACAGAGAAACGCTCTTCTATTTTAACGCGCGAGAAATACCGCCCAAAACAGATAAAAAAAAt                                                         <  2:1485924/93‑1 (MQ=255)
                                 cTCAGGAAAAAATTGCTCAACTTCCTAAAGACAGAGAAACGCTCTTCTATTTTAACGCGCGAGAAATACCGCCCAAAACAGATAAAAAAAAtg                                                        >  1:1502012/1‑93 (MQ=255)
                                 cTCAGGAAAAAATTGCTCAACTTCCTAAAGACAGAGAAACGCTCTTCTATTTTAACGCGCGAGAAATACCGCCCAAAACAGATAAAAAAAAtg                                                        <  2:717035/93‑1 (MQ=255)
                                 cTCAGGAAAAAATTGCTCAACTTCCTAAAGACAGAGAAACGCTCTTCTATTTTAACGCGCGAGAAATACCGCCCAAAACAGATAAAAAAAAtg                                                        <  2:3423712/93‑1 (MQ=255)
                                  tCAGGAAAAAATTGCTCAACTTCCTAAAGACAGAGAAACGCTCTTCTATTTTAACGCGCGAGAAATACCGCCCAAAACAGATAAAAAAAAtgt                                                       >  2:134784/1‑93 (MQ=255)
                                  tCAGGAAAAAATTGCTCAACTTCCTAAAGACAGAGAAACGCTCTTCTATTTTAACGCGCGAGAAATACCGCCCAAAACAGATAAAAAAAAtgt                                                       >  1:1301633/1‑93 (MQ=255)
                                     ggAAAAAATTGCTCAACTTCCTAAAGACAGAGAAACGCTCTTCTATTTTAACGCGCGAGAAATACCGCCCAAAACAGATAAAAAAAATGTGAt                                                    <  1:2143606/93‑1 (MQ=255)
                                       aaaaaaTTGCTCAACTTCCTAAAGACAGAGAAACGCTCTTCTATTTTAACGCGCGAGAAATACCGCCCAAAACAGATAAAAAAAATGTGATga                                                  >  2:4018646/1‑92 (MQ=255)
                                       aaaaaaTTGCTCAACTTCCTAAAGACAGAGAAACGCTCTTCTATTTTAACGCGCGAGAAATACCGCCCAAAACAGATAAAAAAAATGTGATGc                                                  <  1:3196963/93‑1 (MQ=255)
                                         aaaaTTGCTCAACTTCCTAAAGACAGAGAAACGCTCTTCTATTTTAACGCGCGAGAAATACCGCCCAAAACAGATAAAAAAAATGTGATGCaa                                                <  2:3676706/93‑1 (MQ=255)
                                         aaaaTTGCTCAACTTCCTAAAGACAGAGAAACGCTCTTCTATTTTAACGCGCGAGAAATACCGCCCAAAACAGATAAAAAAAATGTGATGCaa                                                <  1:1197030/93‑1 (MQ=255)
                                         aaaaTTGCTCAACTTCCTAAAGACAGAGAAACGCTCTTCTATTTTAACGCGCGAGAAATACCGCCCAAAACAGATAAAAAAAATGTGATGCaa                                                <  2:1897375/93‑1 (MQ=255)
                                          aaaTTGCTCAACTTCCTAAAGACAGAGAAACGCTCTTCTATTTTAACGCGCGAGAAATACCGCCCAAAACAGATAAAAAAAATGTGATGCAAg                                               <  1:3617789/93‑1 (MQ=255)
                                          aaaTTGCTCAACTTCCTAAAGACAGAGAAACGCTCTTCTATTTTAACGCGCGAGAAATACCGCCCAAAACAGATAAAAAAAATGTGATGCAAg                                               <  1:454143/93‑1 (MQ=255)
                                           aaTTGCTCAACTTCCTAAAGACAGAGAAACGCTCTTCTATTTTAACGCGCGAGAAATACCGCCCAAAACAGATAAAAAAAATGTGATGCAAGt                                              >  2:514153/1‑93 (MQ=255)
                                            aTTGCTCAACTTCCTAAAGACAGAGAAACGCTCTTCTATTTTAACGCGCGAGAAATACCGCCCAAAACAGATAAAAAAAATGTGATGCAAGTc                                             >  1:111078/1‑93 (MQ=255)
                                            aTTGCTCAACTTCCTAAAGACAGAGAAACGCTCTTCTATTTTAACGCGCGAGAAATACCGCCCAAAACAGATAAAAAAAATGTGATGCAAGTc                                             >  2:1509930/1‑93 (MQ=255)
                                             ttGCTCAACTTCCTAAAGACAGAGAAACGCTCTTCTATTTTAACGCGCGAGAAATACCGCCCAAAACAGATAAAAAAAATGTGATGCAAGTCa                                            >  2:1629801/1‑93 (MQ=255)
                                               gCTCAACTTCCTAAAGACAGAGAAACGCTCTTCTATTTTAACGCGCGAGAAATACCGCCCAAAACAGATAAAAAAAATGTGATGCAAGTCACt                                          >  2:3405537/1‑93 (MQ=255)
                                                cTCACCTTCCTAAAGACAGAGAAACGCTCTTCTATTTTAGCGCGCGAGAAATACCGCCCAAAACAGAAAAAAAAAATGTGGTGCAAGGCACTa                                         <  2:3554537/93‑1 (MQ=255)
                                                cTCAACTTCCTAAAGACAGAGAAACGCTCTTCTATTTTAACGCGCGAGAAATACCGCCCAAAACAGATAAAAAAAATGTGATGCAAGTCACTa                                         >  2:2252521/1‑93 (MQ=255)
                                                cTCAACTTCCTAAAGACAGAGAAACGCTCTTCTATTTTAACGCGCGAGAAATACCGCCCAAAACAGATAAAAAAAATGTGATGCAAGTCACTa                                         <  1:4658042/93‑1 (MQ=255)
                                                 tCAACTTCCTAAAGACAGAGAAACGCTCTTCTATTTTAACGCGCGAGAAATACCGCCCAGAACAGATAAAAAAAATGTGATGCAAGTCACTAt                                        <  2:3487990/93‑1 (MQ=255)
                                                 tCAACTTCCTAAAGACAGAGAAACGCTCTTCTATTTTAACGCGCGAGAAATACCGCCCAAAACAGATAAAAAAAATGTGATGCAAGTCACTAt                                        <  1:4287687/93‑1 (MQ=255)
                                                   aaCTTCCTAAAGACAGAGAAACGCTCTTCTATTTTAACGCGCGAGAAATACCGCCCAAAACAGATAAAAAAAATGTGATGCAAGTCACTATgc                                      <  2:2214709/93‑1 (MQ=255)
                                                   aaCTTCCTAAAGACAGAGAAACGCTCTTCTATTTTAACGCGCGAGAAATACCGCCCAAAACAGATAAAAAAAATGTGATGCAAGTCACTATgc                                      <  2:1765610/93‑1 (MQ=255)
                                                   aaCTTCCTAAAGACAGAGAAACGCTCTTCTATTTTAACGCGCGAGAAATACCGCCCAAAACAGATAAAAAAAATGTGATGCAAGTCACTATgc                                      >  1:1080250/1‑93 (MQ=255)
                                                    aCTTCCTAAAGACAGAGAAACGCTCTTCTATTTTAACGCGCGAGAAATACCGCCCAAAACAGATAAAAAAAATGTGATGCAAGTCACTATgca                                     >  2:1104011/1‑93 (MQ=255)
                                                    aCTTCCTAAAGACAGAGAAACGCTCTTCTATTTTAACGCGCGAGAAATACCGCCCAAAACAGATAAAAAAAATGTGATGCAAGTCACTATgca                                     >  1:625159/1‑93 (MQ=255)
                                                    aCTTCCTAAAGACAGAGAAACGCTCTTCTATTTTAACGCGCGAGAAATACCGCCCAAAACAGATAAAAAAAATGTGATGCAAGTCACTATgca                                     <  1:275738/93‑1 (MQ=255)
                                                       tCCTAAAGACAGAGAAACGCTCTTCTATTTTAACGCGCGAGAAATACCGCCCAAAACAGATAAAAAAAATGTGATGCAAGTCACTATgcagca                                  <  1:2857302/93‑1 (MQ=255)
                                                         cTAAAGACAGAGAAACGCTCTTCTATTTTAACGCGCGAGAAATACCGCCCAAAACAGATAAAAAAAATGTGATGCAAGTCACTATGCAGCACg                                >  2:2764089/1‑93 (MQ=255)
                                                           aaaGACAGAGAAACGCTCTTCTATTTTAACGCGCGAGAAATACCGCCCAAAACAGATAAAAAAAATGTGATGCAAGTCACTATGCAGCACGCa                              <  2:1369433/93‑1 (MQ=255)
                                                           aaaGACAGAGAAACGCTCTTCTATTTTAACGCGCGAGAAATACCGCCCAAAACAGATAAAAAAAATGTGATGCAAGTCACTATGCAGCACGCa                              >  2:298524/1‑93 (MQ=255)
                                                              gACAGAGAAACGCTCTTCTATTTTAACGCGCGAGAAATACCGCCCAAAACAGATAAAAAAAATGTGATGCAAGTCACTATGCAGCACGCATTg                           <  2:4539752/93‑1 (MQ=255)
                                                              gACAGAGAAACGCTCTTCTATTTTAACGCGCGAGAAATACCGCCCAAAACAGATAAAAAAAATGTGATGCAAGTCACTATGCAGCACGCATTg                           <  2:2516307/93‑1 (MQ=255)
                                                                 agagAAACGCTCTTCTATTTTAACGCGCGAGAAATACCGCCCAAAACAGATAAAAAAAATGTGATGCAAGTCACTATGCAGCACGCATTGaaa                        <  2:3694891/93‑1 (MQ=255)
                                                                  gagaAACGCTCTTCTATTTTAACGCGCGAGAAATACCGCCCAAAACAGATAAAAAAAATGTGATGCAAGTCACTATGCAGCACGCATTGAAAc                       <  2:1937583/93‑1 (MQ=255)
                                                                  gagaAACGCTCTTCTATTTTAACGCGCGAGAAATACCGCCCAAAACAGATAAAAAAAATGTGATGCAAGTCACTATGCAGCACGCATTGAAAc                       <  1:2344682/93‑1 (MQ=255)
                                                                   agaAACGCTCTTCTATTTTAACGCGCGAGAAATACCGCCCAAAACAGATAAAAAAAATGTGATGCAAGTCACTATGCAGCACGCATTGAAACt                      <  1:2050976/93‑1 (MQ=255)
                                                                    gaAACGCTCTTCTATTTTAACGCGCGAGAAATACCGCCCAAAACAGATAAAAAAAATGTGATGCAAGTCACTATGCAGCACGCATTGAAACTa                     <  1:75505/93‑1 (MQ=255)
                                                                    gaAACGCTCTTCTATTTTAACGCGCGAGAAATACCGCCCAAAACAGATAAAAAAAATGTGATGCAAGTCACTATGCAGCACGCATTGAAACTa                     <  2:4438154/93‑1 (MQ=255)
                                                                       aCGCTCTTCTATTTTAACGCGCGAGAAATACCGCCCAAAACAGATAAAAAAAATGTGATGCAAGTCACTATGCAGCACGCATTGAAACTATTc                  >  1:3011584/1‑93 (MQ=255)
                                                                       aCGCTCTTCTATTTTAACGCGCGAGAAATACCGCCCAAAACAGATAAAAAAAATGTGATGCAAGTCACTATGCAGCACGCATTGAAACTATTc                  >  1:3543179/1‑93 (MQ=255)
                                                                        cGCTCTTCTATTTTAACGCGCGAGAAATACCGCCCAAAACAGATAAAAAAAATGTGATGCAAGTCACTATGCAGCACGCATTGAAACTATTCt                 >  1:550510/1‑93 (MQ=255)
                                                                         gCTCTTCTATTTTAACGCGCGAGAAATACCGCCCAAAACAGATAAAAAAAATGTGATGCAAGTCACTATGCAGCACGCATTGAAACTATTCTg                <  2:3981006/93‑1 (MQ=255)
                                                                         gCTCTTCTATTTTAACGCGCGAGAAATACCGCCCAAAACAGATAAAAAAAATGTGATGCAAGTCACTATGCAGCACGCATTGAAACTATTCTg                <  1:4478367/93‑1 (MQ=255)
                                                                           tcttctATTTTAACGCGCGAGAAATACCGCCCAAAACAGATAAAAAAAATGTGATGCAAGTCACTATGCAGCACGCATTGAAACTATTCTggc              >  1:2651252/1‑93 (MQ=255)
                                                                           tcttctATTTTAACGCGCGAGAAATACCGCCCAAAACAGATAAAAAAAATGTGATGCAAGTCACTATGCAGCACGCATTGAAACTATTCTggc              >  1:4046589/1‑93 (MQ=255)
                                                                            cttctATTTTAACGCGCGAGAAATACCGCCCAAAACAGATAAAAAAAATGTGATGCAAGTCACTATGCAGCACGCATTGAAACTATTCTggcg             >  2:3888080/1‑93 (MQ=255)
                                                                             ttctATTTTAACGCGCGAGAAATACCGCCCAAAACAGATAAAAAAAATGTGATGCAAGTCACTATGCAGCACGCATTGAAACTATTCTggcgg            >  1:3056247/1‑93 (MQ=255)
                                                                              tctATTTTAACGCGCGAGAAATACCGCCCAAAACAGATAAAAAAAATGTGATGCAAGTCACTATGCAGCACGCATTGAAACTATTCTggcggc           >  2:444887/1‑93 (MQ=255)
                                                                               ctATTTTAACGCGCGAGAAATACCGCCCAAAACAGATAAAAAAAATGTGATGCAAGTCACTATGCAGCACGCATTGAAACTATTCTGGCGGcc          <  2:4232751/93‑1 (MQ=255)
                                                                               ctATTTTAACGCGCGAGAAATACCGCCCAAAACAGATAAAAAAAATGTGATGCAAGTCACTATGCAGCACGCATTGAAACTATTCTGGCGGcc          <  2:1567571/93‑1 (MQ=255)
                                                                                tATTTTAACGCGCGAGAAATACCGCCCAAAACAGATAAAAAAAATGTGATGCAAGTCACTATGCAGCACGCATTGAAACTATTCTGGCGGCCa         >  1:2200660/1‑93 (MQ=255)
                                                                                  ttttAACGCGCGAGAAATACCGCCCAAAACAGATAAAAAAAATGTGATGCAAGTCACTATGCAGCACGCATTGAAACTATTCTGGCGGCCaaa       >  2:606493/1‑93 (MQ=255)
                                                                                   tttAACGCGCGAGAAATACCGCCCAAAACAGATAAAAAAAATGTGATGCAAGTCACTATGCAGCACGCATTGAAACTATTCTGGCGGCCaaaa      <  1:684428/93‑1 (MQ=255)
                                                                                     tAACGCGCGAGAAATACCGCCCAAAACAGATAAAAAAAATGTGATGCAAGTCACTATGCAGCACGCATTGAAACTATTCTGGCGGCCAAAAGc    <  1:1224510/93‑1 (MQ=255)
                                                                                      aaCGCGCGAGAAATACCGCCCAAAACAGATAAAAAAAATGTGATGCAAGTCACTATGCAGCACGCATTGAAACTATTCTGGCGGCCAAAAGcc   >  2:695020/1‑93 (MQ=255)
                                                                                          |                                                                                         
ATCCCAGTGAGCAAATTCAAATAAGAATGATAACTCAGGAAAAAATTGCTCAACTTCCTAAAGACAGAGAAACGCTCTTCTATTTTAACGTGCGAGAAATACCGCCCAAAACAGATAAAAAAAATGTGATGCAAGTCACTATGCAGCACGCATTGAAACTATTCTGGCGGCCAAAAGCCA  >  NC_000913/3188147‑3188326

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
AAATCCCAGTGAGCAAATTCAAATAAGAATGATAACTCAGGAAAAAATTGCTCAACTTCCTAAAGACAGAGAAACGCTCTTCTATTTTAACGTGCGAGAAATACCGCCCAAAACAGATAAAAAAAATGTGATGCAAGTCACTATGCAGCACGCATTGAAACTATTCTGGCGGCCAAAAGCCATTGA  >  NC_000913/3188145‑3188330
                                                                                            |                                                                                             
AAATCCCAGTGAGCAAATTCAAATAAGAATGATAACTCAGGAAAAAATTGCTCAACTTCCTAAAGACAGAGAAACGCTCTTCTATTTTAACGCG                                                                                              <  SRR4302535.1932212/94‑1 (MQ=60)
AAATCCCAGTGAGCAAATTCAAATAAGAATGATAACTCAGGAAAAAATTGCTCAACTTCCTAAAGACAGAGAAACGCTCTTCTATTTTAACGCG                                                                                              >  SRR4302535.2978976/1‑94 (MQ=60)
 AATCCCAGTGAGCAAATTCAAATAAGAATGATAACTCAGGAAAAAATTGCTCAACTTCCTAAAGACAGAGAAACGCTCTTCTATTTTAACGCGC                                                                                             >  SRR4302535.2828924/1‑94 (MQ=60)
 AATCCCAGTGAGCAAATTCAAATAAGAATGATAACTCAGGAAAAAATTGCTCAACTTCCTAAAGACAGAGAAACGCTCTTCTATTTTAACGCGC                                                                                             >  SRR4302535.1940929/1‑94 (MQ=60)
  ATCCCAGTGAGCAAATTCAAATAAGAATGATAACTCAGGAAAAAATTGCTCAACTTCCTAAAGACAGAGAAACGCTCTTCTATTTTAACGCGCG                                                                                            >  SRR4302535.4285813/1‑94 (MQ=60)
  ATCCCAGTGAGCAAATTCAAATAAGAATGATAACTCAGGAAAAAATTGCTCAACTTCCTAAAGACAGAGAAACGCTCTTCTATTTTAACGCGCG                                                                                            >  SRR4302535.1937637/1‑94 (MQ=60)
  ATCCCAGTGAGCAAATTCAAATAAGAATGATAACTCAGGAAAAAATTGCTCAACTTCCTAAAGACAGAGAAACGCTCTTCTATTTTAACGCGCG                                                                                            >  SRR4302535.658585/1‑94 (MQ=60)
  ATCCCAGTGAGCAAATTCAAATAAGAATGATAACTCAGGAAAAAATTGCTCAACTTCCTAAAGACAGAGAAACGCTCTTCTATTTTAACGCGCG                                                                                            <  SRR4302535.3910389/94‑1 (MQ=60)
  ATCCCAGTGAGCAAATTCAAATAAGAATGATAACTCAGGAAAAAATTGCTCAACTTCCTAAAGACAGAGAAACGCTCTTCTATTTTAACGCGCG                                                                                            >  SRR4302535.2981661/1‑94 (MQ=60)
    CCCAGTGAGCAAATTCAAATAAGAATGATAACTCAGGAAAAAATTGCTCAACTTCCTAAAGACAGAGAAACGCTCTTCTATTTTAACGCGCGAG                                                                                          >  SRR4302535.3352461/1‑94 (MQ=60)
     CCAGTGAGCAAATTCAAATAAGAATGATAACTCAGGAAAAAATTGCTCAACTTCCTAAAGACAGAGAAACGCTCTTCTATTTTAACGCGCGAGA                                                                                         >  SRR4302535.724092/1‑94 (MQ=60)
      CAGTGAGCAAATTCAAATAAGAATGATAACTCAGGAAAAAATTGCTCAACTTCCTAAAGACAGAGAAACGCTCTTCTATTTTAACGCGCGAGAA                                                                                        <  SRR4302535.2300983/94‑1 (MQ=60)
      CAGTGAGCAAATTCAAATAAGAATGATAACTCAGGAAAAAATTGCTAAACTTCCTAAAGACAGAGAAACGCTCTTCTATTTTAACGCGCGAGAA                                                                                        >  SRR4302535.1818253/1‑94 (MQ=60)
        GTGAGCAAATTCAAATAAGAATGATAACTCAGGAAAAAATTGCTCAACTTCCTAAAGACAGAGAAACGCTCTTCTATTTTAACGCGCGAGAAAT                                                                                      <  SRR4302535.3434827/94‑1 (MQ=60)
        GTGAGCAAATTCAAATAAGAATGATAACTCAGGAAAAAATTGCTCAACTTCCTAAAGACAGAGAAACGCTCTTCTATTTTAACGCGCGAGAAAT                                                                                      <  SRR4302535.3313125/94‑1 (MQ=60)
        GTGAGCAAATTCAAATAAGAATGATAACTCAGGAAAAAATTGCTCAACTTCCTAAAGACAGAGAAACGCTCTTCTATTTTAACGCGCGAGAAAT                                                                                      <  SRR4302535.2994548/94‑1 (MQ=60)
        GTGAGCAAATTCAAATAAGAATGATAACTCAGGAAAAAATTGCTCAACTTCCTAAAGACAGAGAAACGCTCTTCTATTTTAACGCGCGAGAAAT                                                                                      <  SRR4302535.1708058/94‑1 (MQ=60)
        GTGAGCAAATTCAAATAAGAATGATAACTCAGGAAAAAATTGCTCAACTTCCTAAAGACAGAGAAACGCTCTTCTATTTTAACGCGCGAGAAAT                                                                                      >  SRR4302535.2610801/1‑94 (MQ=60)
         TGAGCAAATTCAAATAAGAATGATAACTCAGGAAAAAATTGCTCAACTTCCTAAAGACAGAGAAACGCTCTTCTATTTTAACGCGCGAGAAATA                                                                                     >  SRR4302535.9346/1‑94 (MQ=60)
          GAGCAAATTCAAATAAGAATGATAACTCAGGAAAAAATTGCTCAACTTCCTAAAGACAGAGAAACGCTCTTCTATTTTAACGCGCGAGAAATAC                                                                                    <  SRR4302535.3803743/94‑1 (MQ=60)
           AGCAAATTCAAATAAGAATGATAACTCAGGAAAAAATTGCTCAACTTCCTAAAGACAGAGAAACGCTCTTCTATTTTAACGCGCGAGAAATACC                                                                                   <  SRR4302535.3730100/94‑1 (MQ=60)
           AGCAAATTCAAATAAGAATGATAACTCAGGAAAAAATTGCTCAACTTCCTAAAGACAGAGAAACGCTCTTCTATTTTAACGCGCGAGAAATACC                                                                                   >  SRR4302535.1677696/1‑94 (MQ=60)
            GCAAATTCAAATAAGAATGATAACTCAGGAAAAAATTGCTCAACTTCCTAAAGACAGAGAAACGCTCTTCTATTTTAACGCGCGAGAAATACCG                                                                                  >  SRR4302535.846078/1‑94 (MQ=60)
              AAATTCAAATAAGAATGATAACTCAGGAAAAAATTGCTCAACTTCCTAAAGACAGAGAAACGCTCTTCTATTTTAACGCGCGAGAAATACCGCC                                                                                >  SRR4302535.543267/1‑94 (MQ=60)
               AATTCAAATAAGAATGATAACTCAGGAAAAAATTGCTCAACTTCCTAAAGACAGAGAAACGCTCTTCTATTTTAACGCGCGAGAAATACCGCCC                                                                               >  SRR4302535.1138475/1‑94 (MQ=60)
               AATTCAAATAAGAATGATAACTCAGGAAAAAATTGCTCAACTTCCTAAAGACAGAGAAACGCTCTTCTATTTTAACGCGCGAGAAATACCGCCC                                                                               <  SRR4302535.486211/94‑1 (MQ=60)
                ATTCAAATAAGAATGATAACTCAGGAAAAAATTGCTCAACTTCCTAAAGACAGAGAAACGCTCTTCTATTTTAACGCGCGAGAAATACCGCCCA                                                                              >  SRR4302535.3751968/1‑94 (MQ=60)
                 TTCAAATAAGAATGATAACTCAGGAAAAAATTGCTCAACTTCCTAAAGACAGAGAAACGCTCTTCTATTTTAACGCGCGAGAAATACCGCCCAA                                                                             >  SRR4302535.3456687/1‑94 (MQ=60)
                  TCAAATAAGAATGATAACTCAGGAAAAAATTGCTCAACTTCCTAAAGACAGAGAAACGCTCTTCTATTTTAACGCGCGAGAAATACCGCCCAAA                                                                            >  SRR4302535.4232445/1‑94 (MQ=60)
                  TCAAATAAGAATGATAACTCAGGAAAAAATTGCTCAACTTCCTAAAGACAGAGAAACGCTCTTCTATTTTAACGCGCGAGAAATACCGCCCAAA                                                                            >  SRR4302535.1813917/1‑94 (MQ=60)
                   CAAATAAGAATGATAACTCAGGAAAAAATTGCTCAACTTCCTAAAGACAGAGAAACGCTCTTCTATTTTAACGCGCGAGAAATACCGCCCAAAA                                                                           >  SRR4302535.4482341/1‑94 (MQ=60)
                    AAATAAGAATGATAACTCAGGAAAAAATTGCTCAACTTCCTAAAGACAGAGAAACGCTCTTCTATTTTAACGCGCGAGAAATACCGCCCAAAAC                                                                          <  SRR4302535.1720073/94‑1 (MQ=60)
                    AAATAAGAATGATAACTCAGGAAAAAATTGCTCAACTTCCTAAAGACAGAGAAACGCTCTTCTATTTTAACGCGCGAGAAATACCGCCCAAAAC                                                                          <  SRR4302535.3741105/94‑1 (MQ=60)
                    AAATAAGAATGATAACTCAGGAAAAAATTGCTCAACTTCCTAAAGACAGAGAAACGCTCTTCTATTTTAACGCGCGAGAAATACCGCCCAAAAC                                                                          >  SRR4302535.613976/1‑94 (MQ=60)
                    AAATAAGAATGATAACTCAGGAAAAAATTGCTCAACTTCCTAAAGACAGAGAAACGCTCTTCTATTTTAACGCGCGAGAAATACCGCCCAAAAC                                                                          >  SRR4302535.4013797/1‑94 (MQ=60)
                     AATAAGAATGATAACTCAGGAAAAAA‑TGCTCAAATTCCTAAAGACAGAGAAAAGCTCTTCTATTTTAACGCGCGAGAAATACCGCCCAAAACAA                                                                        >  SRR4302535.538525/1‑94 (MQ=60)
                      ATAAGAATGATAACTCAGGAAAAAATTGCTCAACTTCCTAAAGACAGAGAAACGCTCTTCTATTTTAACGCGCGAGAAATACCGCCCAAAACAG                                                                        >  SRR4302535.279096/1‑94 (MQ=60)
                       TAAGAATGATAACTCAGGAAAAAATTGCTCAACTTCCTAAAGACAGAGAAACGCTCTTCTATTTTAACGCGCGAGAAATACCGCCCAAAACAGA                                                                       >  SRR4302535.4706830/1‑94 (MQ=60)
                       TAAGAATGATAACTCAGGAAAAAATTGCTCAACTTCCTAAAGACAGAGAAACGCTCTTCTATTTTAACGCGCGAGAAATACCGCCCAAAACAGA                                                                       <  SRR4302535.2078614/94‑1 (MQ=60)
                       TAAGAATGATAACTCAGGAAAAAATTGCTCAACTTCCTAAAGACAGAGAAACGCTCTTCTATTTTAACGCGCGAGAAATACCGCCCAAAACAGA                                                                       <  SRR4302535.3764133/94‑1 (MQ=60)
                        AAGAATGATAACTCAGGAAAAAATTGCTCAACTTCCTAAAGACAGAGAAACGCTCTTCTATTTTAACGCGCGAGAAATACCGCCCAAAACAGAT                                                                      >  SRR4302535.1241972/1‑94 (MQ=60)
                        AAGAATGATAACTCAGGAAAAAATTGCTCAACTTCCTAAAGACAGAGAAACGCTCTTCTATTTTAACGCGCGAGAAATACCGCCCAAAACAGAT                                                                      <  SRR4302535.3796850/94‑1 (MQ=60)
                         AGAATGATAACTCAGGAAAAAATTGCTCAACTTCCTAAAGACAGAGAAACGCTCTTCTATTTTAACGCGCGAGAAATACCGCCCAAAACAGATA                                                                     <  SRR4302535.842033/94‑1 (MQ=60)
                          GAATGATAACTCAGGAAAAAATTGCTCAACTTCCTAAAGACAGAGAAACGCTCTTCTATTTTAACGCGCGAGAAATACCGCCCAAAACAGATAA                                                                    <  SRR4302535.4625117/94‑1 (MQ=60)
                          GAATGATAACTCAGGAAAAAATTGCTCAACTTCCTAAAGACAGAGAAACGCTCTTCTATTTTAACGCGCGAGAAATACCGCCCAAAACAGATAA                                                                    >  SRR4302535.3087911/1‑94 (MQ=60)
                          GAATGATAACTCAGGAAAAAATTGCTCAACTTCCTAAAGACAGAGAAACGCTCTTCTATTTTAACGCGCGAGAAATACCGCCCAAAACAGATAA                                                                    >  SRR4302535.2589172/1‑94 (MQ=60)
                            ATGATAACTCAGGAAAAAATTGCTCAACTTCCTAAAGACAGAGAAACGCTCTTCTATTTTAACGCGCGAGAAATACCGCCCAAAACAGATAAAA                                                                  >  SRR4302535.2306740/1‑94 (MQ=60)
                             TGATAACTCAGGAAAAAATTGCTCAACTTCCTAAAGACAGAGAAACGCTCTTCTATTTTAACGCGCGAGAAATACCGCCCAAAACAGATAAAAA                                                                 <  SRR4302535.2372122/94‑1 (MQ=60)
                             TGATAACTCAGGAAAAAATTGCTCAACTTCCTAAAGACAGAGAAACGCTCTTCTATTTTAACGCGCGAGAAATACCGCCCAAAACAGATAAAAA                                                                 >  SRR4302535.1954055/1‑94 (MQ=60)
                             TGATAACTCAGGAAAAAAGTGCTCAACTTCCTAAAGACAGAGAAACGCTCTTCTATTTTAACGCGCGAGAAATACCGCCCAAAACAGATAAAAA                                                                 <  SRR4302535.1293321/94‑1 (MQ=60)
                              GATATCTCAGGAAAAAATTGCTCAACTTCCTAAAGACAGAGAAACGCTCTTCTATTTTAACGCGCGAGAAATACCGCCCAAAACAGATAAAAAA                                                                <  SRR4302535.213994/94‑1 (MQ=60)
                              GATAACTCAGGAAAAAATTGCTCAACTTCCTAAAGACAGAGAAACGCTCTTCTATTTTAACGCGCGAGAAATACCGCCCAAAACAGATAAAAAA                                                                <  SRR4302535.4192577/94‑1 (MQ=60)
                               ATAACTCAGGAAAAAATTGCTCAACTTCCTAAAGACAGAGAAACGCTCTTCTATTTTAACGCGCGAGAAATACCGCCCAAAACAGATAAAAAAA                                                               >  SRR4302535.2438422/1‑94 (MQ=60)
                                TAACTCAGGAAAAAATTGCTCAACTTCCTAAAGACAGAGAAACGCTCTTCTATTTTAACGCGCGAGAAATACCGCCCAAAACAGATAAAAAAAA                                                              <  SRR4302535.3202509/94‑1 (MQ=60)
                                 AACTCAGGAAAAAATTGCTCAACTTCCTAAAGACAGAGAAACGCTCTTCTATTTTAACGCGCGAGAAATACCGCCCAAAACAGATAAAAAAAAT                                                             <  SRR4302535.1215072/94‑1 (MQ=60)
                                 AACTCAGGAAAAAATTGCTCAACTTCCTAAAGACAGAGAAACGCTCTTCTATTTTAACGCGCGAGAAATACCGCCCAAAACAGATAAAAAAAAT                                                             <  SRR4302535.3636970/94‑1 (MQ=60)
                                 AACTCAGGAAAAAATTGCTCAACTTCCTAAAGACAGAGAAACGCTCTTCTATTTTAACGCGCGAGAAATACCGCCCAAAACAGATAAAAAAAAT                                                             <  SRR4302535.2484140/94‑1 (MQ=60)
                                  ACTCAGGAAAAAATTGCTCAACTTCCTAAAGACAGAGAAACGCTCTTCTATTTTAACGCGCGAGAAATACCGCCCAAAACAGATAAAAAAAATG                                                            >  SRR4302535.1512818/1‑94 (MQ=60)
                                   CTCAGGAAAAAATTGCTCAACTTCCTAAAGACAGAGAAACGCTCTTCTATTTTAACGCGCGAGAAATACCGCCCAAAACAGATAAAAAAAATGT                                                           <  SRR4302535.3299510/94‑1 (MQ=60)
                                   CTCAGGAAAAAATTGCTCAACTTCCTAAAGACAGAGAAACGCTCTTCTATTTTAACGCGCGAGAAATACCGCCCAAAACAGATAAAAAAAATGT                                                           >  SRR4302535.1310931/1‑94 (MQ=60)
                                   CTCAGGAAAAAATTGCTCAACTTCCTAAAGACAGAGAAACGCTCTTCTATTTTAACGCGCGAGAAATACCGCCCAAAACAGATAAAAAAAATGT                                                           <  SRR4302535.3044852/94‑1 (MQ=60)
                                   CTCAGGAAAAAATTGCTCAACTTCCTAAAGACAGAGAAACGCTCTTCTATTTTAACGCGCGAGAAATACCGCCCAAAACAGATAAAAAAAATGT                                                           >  SRR4302535.2375686/1‑94 (MQ=60)
                                   CTCAGGAAAAAATTGCTCAACTTCCTAAAGACAGAGAAACGCTCTTCTATTTTAACGCGCGAGAAATACCGCCCAAAACAGATAAAAAAAATGT                                                           <  SRR4302535.4277956/94‑1 (MQ=60)
                                     CAGGAAAAAATTGCTCAACTTCCTAAAGACAGAGAAACGCTCTTCTATTTTAACGCGCGAGAAATACCGCCCAAAACAGATAAAAAAAATGTGA                                                         <  SRR4302535.768915/94‑1 (MQ=60)
                                      AGGAAAAAATTGCTCAACTTCCTAAAGACAGAGAAACGCTCTTCTATTTTAACGCGCGAGAAATACCGCCCAAAACAGATAAAAAAAATGTGAT                                                        >  SRR4302535.4543896/1‑94 (MQ=60)
                                      AGGAAAAAATTGCTCAACTTCCTAAAGACAGAGAAACGCTCTTCTATTTTAACGCGCGAGAAATACCGCCCAAAACAGATAAAAAAAATGTGAT                                                        >  SRR4302535.3697019/1‑94 (MQ=60)
                                       GGAAAAAATTGCTCAACTTCCTAAAGACAGAGAAACGCTCTTCTATTTTAACGCGCGAGAAATACCGCCCAAAACAGATAAAAAAAATGTGATG                                                       <  SRR4302535.118402/94‑1 (MQ=60)
                                       GGAAAAAATTGCTCAACTTCCTAAAGACAGAGAAACGCTCTTCTATTTTAACGCGCGAGAAATACCGCCCAAAACAGATAAAAAAAATGTGATG                                                       <  SRR4302535.2159025/94‑1 (MQ=60)
                                         AAAAAATTGCTCAACTTCCTAAAGACAGAGAAACGCTCTTCTATTTTAACGCGCGAGAAATACCGCCCAAAACAGATAAAAAAAATGTGATGCA                                                     <  SRR4302535.696696/94‑1 (MQ=60)
                                         AAAAAATTGCTCAACTTCCTAAAGACAGAGAAACGCTCTTCTATTTTAACGCGCGAGAAATACCGCCCAAAACAGATAAAAAAAATGTGATGCA                                                     <  SRR4302535.3219086/94‑1 (MQ=60)
                                         AAAAAATTGCTCAACTTCCTAAAGACAGAGAAACGCTCTTCTATTTTAACGCGCGAGAAATACCGCCCAAAACAGATAAAAAAAATGTGATGCA                                                     >  SRR4302535.1617013/1‑94 (MQ=60)
                                         AAAAAATTGCTCAACTTCCTAAAGACAGAGAAACGCTCTTCTATGTTAACGCGCGAGAAATACCGCCCAAAACAGATAAAAAAAATGTGATGCA                                                     <  SRR4302535.2702892/94‑1 (MQ=60)
                                          AAAAATTGCTCAACTTCCTAAAGACAGAGAAACGCTCTTCTATTTTAACGCGCGAGAAATACCGCCCAAAACAGATAAAAAAAATGTGATGCAA                                                    >  SRR4302535.4637147/1‑94 (MQ=60)
                                           AAAATTGCTCAACTTCCTAAAGACAGAGAAACGCTCTTCTATTTTAACGCGCGAGAAATACCGCCCAAAACAGATAAAAAAAATGTGATGCAAG                                                   <  SRR4302535.648329/94‑1 (MQ=60)
                                           AAAATTGCTCAACTTCCTAAAGACAGAGAAACGCTCTTCTATTTTAACGCGCGAGAAATACCGCCCAAAACAGATAAAAAAAATGTGATGCAAG                                                   <  SRR4302535.293312/94‑1 (MQ=60)
                                           AAAATTGCTCAACTTCCTAAAGACAGAGAAACGCTCTTCTATTTTAACGCGCGAGAAATACCGCCCAAAACAGATAAAAAAAATGTGATGCAAG                                                   <  SRR4302535.1205601/94‑1 (MQ=60)
                                            AAATTGCTCAACTTCCTAAAGACAGAGAAACGCTCTTCTATTTTAACGCGCGAGAAATACCGCCCAAAACAGATAAAAAAAATGTGATGCAAGT                                                  <  SRR4302535.3891472/94‑1 (MQ=60)
                                            AAATTGCTCAACTTCCTAAAGACAGAGAAACGCTCTTCTATTTTAACGCGCGAGAAATACCGCCCAAAACAGATAAAAAAAATGTGATGCAAGT                                                  <  SRR4302535.3642869/94‑1 (MQ=60)
                                            AAATTGCTCAACTTCCTAAAGACAGAGAAACGCTCTTCTATTTTAACGCGCGAGAAATACCGCCCAAAACAGATAAAAAAAATGTGATGCAAGT                                                  <  SRR4302535.457326/94‑1 (MQ=60)
                                             AATTGCTCAACTTCCTAAAGACAGAGAAACGCTCTTCTATTTTAACGCGCGAGAAATACCGCCCAAAACAGATAAAAAAAATGTGATGCAAGTC                                                 <  SRR4302535.3258540/94‑1 (MQ=60)
                                             AATTGCTCAACTTCCTAAAGACAGAGAAACGCTCTTCTATTTTAACGCGCGAGAAATACCGCCCAAAACAGATAAAAAAAATGTGATGCAAGTC                                                 >  SRR4302535.2543153/1‑94 (MQ=60)
                                             AATTGCTCAACTTCCTAAAGACAGAGAAACGCTCTTCTATTTTAACGCGCGAGAAATACCGCCCAAAACAGATAAAAAAAATGTGATGCAAGTC                                                 <  SRR4302535.4575023/94‑1 (MQ=60)
                                             AATTGCTCAACTTCCTAAAGACAGAGAAACGCTCTTCTATTTTAACGCGCGAGAAATACCGCCCAAAACAGATAAAAAAAATGTGATGCAAGTC                                                 >  SRR4302535.111861/1‑94 (MQ=60)
                                                TGCTCAACTTCCTAAAGACAGAGAAACGCTCTTCTATTTTAACGCGCGAGAAATACCGCCCAAAACAGATAAAAAAAATGTGATGCAAGTCACT                                              >  SRR4302535.305667/1‑94 (MQ=60)
                                                  CTCAACTTCCTAAAGACAGAGAAACGCTCTTCTATTTTAACGCGCGAGAAATACCGCCCAAAACAGATAAAAAAAATGTGATGCAAGTCACTAT                                            <  SRR4302535.4689555/94‑1 (MQ=60)
                                                  CTCAACTTCCTAAAGACAGAGAAACGCTCTTCTATTTTAACGCGCGAGAAATACCGCCCAAAACAGATAAAAAAAATGTGATGCAAGTCACTAT                                            <  SRR4302535.1109644/94‑1 (MQ=60)
                                                   TCAACTTCCTAAAGACAGAGAAACGCTCTTCTATTTTAACGCGCGAGAAATACCGCCCAAAACAGATAAAAAAAATGTGATGCAAGTCACTATG                                           <  SRR4302535.979505/94‑1 (MQ=60)
                                                   TCAACTTCCTAAAGACAGAGAAACGCTCTTCTATTTTAACGCGCGAGAAATACCGCCCAAAACAGATAAAAAAAATGTGATGCAAGTCACTATG                                           <  SRR4302535.4317020/94‑1 (MQ=60)
                                                    CAACTTCCTAAAGACAGAGAAACGCTCTTCTATTTTAACGCGCGAGAAATACCGCCCAAAACAGATAAAAAAAATGTGATGCAAGTCACTATGC                                          >  SRR4302535.1087935/1‑94 (MQ=60)
                                                    CAACTTCCTAAAGACAGAGAAACGCTCTTCTATTTTAACGCGCGAGAAATACCGCCCAAAACAGATAAAAAAAATGTGATGCAAGTCACTATGC                                          >  SRR4302535.62749/1‑94 (MQ=60)
                                                     AACTTCCTAAAGACAGAGAAACGCTCTTCTATTTTAACGCGCGAGAAATACCGCCCAAAACAGATAAAAAAAATGTGATGCAAGTCACTATGCA                                         >  SRR4302535.4657688/1‑94 (MQ=60)
                                                     AACTTCCTAAAGACAGAGAAACGCTCTTCTATTTTAACGCGCGAGAAATACCGCCCAAAACAGATAAAAAAAATGTGATGCAAGTCACTATGCA                                         >  SRR4302535.629507/1‑94 (MQ=60)
                                                      ACTTCCTAAAGACAGAGAAACGCTCTTCTATTTTAACGCGCGAGAAATACCGCCCAAAACAGATAAAAAAAATGTGATGCAAGTCACTATGCAG                                        <  SRR4302535.277661/94‑1 (MQ=60)
                                                      ACTTCCTAAAGACAGAGAAACGCTCTTCTATTTTAACGCGCGAGAAATACCGCCCAAAACAGATAAAAAAAATGTGATGCAAGTCACTATGCAG                                        <  SRR4302535.4488500/94‑1 (MQ=60)
                                                       CTTCCTAAAGACAGAGAAACGCTCTTCTATTTTAACGCGCGAGAAATACCGCCCAAAACAGATAAAAAAAATGggttcaagtcactattgagcc                                       >  SRR4302535.3277550/1‑73 (MQ=60)
                                                       CTTCCTAAAGACAGAGAAACGCTCTTCTATTTTAACGCGCGAGAAATACCGCCCAAAACAGATAAAAAAAATGTGATGCAAGTCACTATGCAGC                                       >  SRR4302535.1248157/1‑94 (MQ=60)
                                                       ATTCCTAAAGACAGAAGAACGCTCCTCTATTTTAACGCGCGAGGAATTCCGCCCAAAACAGATAAAAAAAATGTGATGCAAGTCACTATGCAGC                                       <  SRR4302535.4198233/94‑1 (MQ=60)
                                                         TCCTAAAGACAGAGAAACGCTCTTCTATTTTAACGCGCGAGAAATACCGCCCAAAACAGATAAAAAAAATGTGATGCAAGTCACTATGCAGCAC                                     <  SRR4302535.2877544/94‑1 (MQ=60)
                                                            TAAAGACAGAGAAACGCTCTTCTATTTTAACGCGCGAGAAATACCGCCCAAAACAGATAAAAAAAATGTGATGCAAGTCACTATGCAGCACGCA                                  >  SRR4302535.4169140/1‑94 (MQ=60)
                                                             AAAGACAGAGAAACGCTCTTCTATTTTAACGCGCGAGAAATACCGCCCAAAACAGATAAAAAAAATGTGATGCAAGTCACTATGCAGCACGCAT                                 <  SRR4302535.3420346/94‑1 (MQ=60)
                                                                  CAGAGAAACGCTCTTCTATTTTAACGCGCGAGAAATACCGCCCAAAACAGATAAAAAAAATGTGATGCAAGTCACTATGCAGCACGCATTGAAA                            <  SRR4302535.2702294/94‑1 (MQ=60)
                                                                   AGAGAAACGCTCTTCTATTTTAACGCGCGAGAAATACCGCCCAAAACAGATAAAAAAAATGTGATGCAAGTCACTATGCAGCACGCATTGAAAC                           >  SRR4302535.3381869/1‑94 (MQ=60)
                                                                    GAGAAACGCTCTTCTATTTTAACGCGCGAGAAATACCGCCCAAAACAGATAAAAAAAATGTGATGCAAGTCACTATGCAGCACGCATTGAAACT                          <  SRR4302535.2361480/94‑1 (MQ=60)
                                                                    GAGAAACGCTCTTCTATTTTAACGCGCGAGAAATACCGCCCAAAACAGATAAAAAAAATGTGATGCAAGTCACTATGCAGCACGCATTGAAACT                          <  SRR4302535.1710203/94‑1 (MQ=60)
                                                                    GAGAAACGCTCTTCTATTTTAACGCGCGAGAAATACCGCCCAAAACAGATAAAAAAAATGTGATGCAAGTCACTATGCAGCACGCATTGAAACT                          <  SRR4302535.4380680/94‑1 (MQ=60)
                                                                     AGAAACGCTCTTCTATTTTAACGCGCGAGAAATACCGCCCAAAACAGATAAAAAAAATGTGATGCAAGTCACTATGCAGCACGCATTGAAACTA                         >  SRR4302535.4385818/1‑94 (MQ=60)
                                                                     AGAAACGCTCTTCTATTTTAACGCGCGAGAAATACCGCCCAAAACAGATAAAAAAAATGTGATGCAAGTCACTATGCAGCACGCATTGAAACTA                         <  SRR4302535.2065745/94‑1 (MQ=60)
                                                                     AGAAACGCTCTTCTATTTTAACGCGCGAGAAATACCGCCCAAAACAGATAAAAAAAATGTGATGCAAGTCACTATGCAGCACGCATTGAAACTA                         >  SRR4302535.1223198/1‑94 (MQ=60)
                                                                      GAAACGCTCTTCTATTTTAACGCGCGAGAAATACCGCCCAAAACAGATAAAAAAAATGTGATGCAAGTCACTATGCAGCACGCATTGAAACTAT                        <  SRR4302535.76056/94‑1 (MQ=60)
                                                                       AAACGCTCTTCTATTTTAACGCGCGAGAAATACCGCCCAAAACAGATAAAAAAAATGTGATGCAAGTCACTATGCAGCACGCATTGAAACTATT                       >  SRR4302535.1733506/1‑94 (MQ=60)
                                                                        AACGCTCTTCTATTTTAACGCGCGAGAAATACCGCCCAAAACAGATAAAAAAAATGTGATGCAAGTCACTATGCAGCACGCATTGAAACTATTC                      >  SRR4302535.3567793/1‑94 (MQ=60)
                                                                        AACGCTCTTCTATTTTAACGCGCGAGAAATACCGCCCAAAACAGATAAAAAAAATGTGATGCAAGTCACTATGCAGCACGCATTGAAACTATTC                      >  SRR4302535.3032685/1‑94 (MQ=60)
                                                                        AACGCTCTTCTATTTTAACGCGCGAGAAATACCGCCCAAAACAGATAAAAAAAATGTGATGCAAGTCACTATGCAGCACGCATTGAAACTATTC                      <  SRR4302535.3574594/94‑1 (MQ=60)
                                                                         ACGCTCTTCTATTTTAACGCGCGAGAAATACCGCCCAAAACAGATAAAAAAAATGTGATGCAAGTCACTATGCAGCACGCATTGAAACTATTCT                     >  SRR4302535.554361/1‑94 (MQ=60)
                                                                           GCTCTTCTATTTTAACGCGCGAGAAATACCGCCCAAAACAGATAAAAAAAATGTGATGCAAGTCACTATGCAGCACGCATTGAAACTATTCTGG                   <  SRR4302535.4509114/94‑1 (MQ=60)
                                                                           GCTCTTCTATTTTAACGCGCGAGAAATACCGCCCAAAACAGATAAAAAAAATGTGATGCAAGTCACTATGCAGCACGCATTGAAACTATTCTGG                   <  SRR4302535.3989045/94‑1 (MQ=60)
                                                                           GCTCTTCTATTTTAACGCGCGAGAAATACCGCCCAAAACAGATAAAAAAAATGTGATGCAAGTCACTATGCAGCACGCATTGAAACTATTCTGG                   <  SRR4302535.2165960/94‑1 (MQ=60)
                                                                            CTCTTCTATTTTAACGCGCGAGAAATACCGCCCAAAACAGATAAAAAAAATGTGATGCAAGTCACTATGCAGCACGCATTGAAACTATTCTGGC                  >  SRR4302535.4583134/1‑94 (MQ=60)
                                                                            CTCTTCTATTTTAACGCGCGAGAAATACCGCCCAAAACAGATAAAAAAAATGTGATGCAAGTCACTATGCAGCACGCATTGAAACTATTCTGGC                  <  SRR4302535.3870098/94‑1 (MQ=60)
                                                                            CTCTTCTATTTTAACGCGCGAGAAATACCGCCCAAAACAGATAAAAAAAATGTGATGCAAGTCACTATGCAGCACGCATTGAAACTATTCTGGC                  >  SRR4302535.4074288/1‑94 (MQ=60)
                                                                            CTCTTCTATTTTAACGCGCGAGAAATACCGCCCAAAACAGATAAAAAAAATGTGATGCAAGTCACTATGCAGCACGCATTGAAACTATTCTGGC                  <  SRR4302535.3718415/94‑1 (MQ=60)
                                                                            CTCTTCTATTTTAACGCGCGAGAAATACCGCCCAAAACAGATAAAAAAAATGTGATGCAAGTCACTATGCAGCACGCATTGAAACTATTCTGGC                  >  SRR4302535.2670247/1‑94 (MQ=60)
                                                                              CTTCTATTTTAACGCGCGAGAAATACCGCCCAAAACAGATAAAAAAAATGTGATGCAAGTCACTATGCAGCACGCATTGAAACTATTCTGGCGG                >  SRR4302535.3077637/1‑94 (MQ=60)
                                                                                 CTATTTTAACGCGCGAGAAATACCGCCCAAAACAGATAAAAAAAATGTGATGCAAGTCACTATGCAGCACGCATTGAAACTATTCTGGCGGCCA             >  SRR4302535.2216347/1‑94 (MQ=60)
                                                                                 CTATTTTAACGCGCGAGAAATACCGCCCAAAACAGATAAAAAAAATGTGATGCAAGTCACTATGCAGCACGCATTGAAACTATTCTGGCGGCCA             >  SRR4302535.1298531/1‑94 (MQ=60)
                                                                                 CTATTTTAACGCGCGAGAAATACCGCCCAAAACAGATAAAAAAAATGTGATGCAAGTCACTATGCAGCACGCATTGAAACTATTCTGGCGGCCA             >  SRR4302535.4133389/1‑94 (MQ=60)
                                                                                  TATTTTAACGCGCGAGAAATACCGCCCAAAACAGATAAAAAAAATGTGATGCAAGTCACTATGCAGCACGCATTGAAACTATTCTGGCGGCCAA            >  SRR4302535.1362196/1‑94 (MQ=60)
                                                                                   ATTTTAACGCGCGAGAAATACCGCCCAAAACAGATAAAAAAAATGTGATGCAAGTCACTATGCAGCACGCATTGAAACTATTCTGGCGGCAAAA           >  SRR4302535.2048839/1‑94 (MQ=60)
                                                                                    TTTTAACGCGCGAGAAATACCGCCCAAAACAGATAAAAAAAATGTGATGCAAGTCACTATGCAGCACGCATTGAAACTATTCTGGCGGCCAAAA          <  SRR4302535.4489802/94‑1 (MQ=60)
                                                                                     TTTAACGCGCGAGAAATACCGCCCAAAACAGATAAAAAAAATGTGATGCAAGTCACTATGCAGCACGCATTGAAACTATTCTGGCGGCCAAAAG         <  SRR4302535.689220/94‑1 (MQ=60)
                                                                                       TAACGCGCGAGAAATACCGCCCAAAACAGATAAAAAAAATGTGATGCAAGTCACTATGCAGCACGCATTGAAACTATTCTGGCGGCCAAAAGCC       <  SRR4302535.1233269/94‑1 (MQ=60)
                                                                                       TAACGCGCGAGAAATACCGCCCAAAACAGATAAAAAAAATGTGATGCAAGTCACTATGCAGCACGCATTGAAACTATTCTGGCGGCCAAAAGCC       <  SRR4302535.900285/94‑1 (MQ=60)
                                                                                        AACGCGCGAGAAATACCGCCCAAAACAGATAAAAAAAATGTGATGCAAGTCACTATGCAGCACGCATTGAAACTATTCTGGCGGCCAAAAGCCA      <  SRR4302535.1606819/94‑1 (MQ=60)
                                                                                        AACGCGCGAGAAATACCGCCCAAAACAGATAAAAAAAATGTGATGCAAGTCACTATGCAGCACGCATTGAAACTATTCTGGCGGCCAAAAGCCA      >  SRR4302535.1430734/1‑94 (MQ=60)
                                                                                        AACGCGCGAGAAATACCGCCCAAAACAGATAAAAAAAATGTGATGCAAGTCACTATGCAGCACGCATTGAAACTATTCTGGCGGCCAAAAGCCA      <  SRR4302535.2924961/94‑1 (MQ=60)
                                                                                          CGCGCGAGAAATACCGCCCAAAACAGATAAAAAAAATGTGATGCAAGTCACTATGCAGCACGCATTGAAACTATTCTGGCGGCCAAAAGCCATT    <  SRR4302535.1301806/94‑1 (MQ=60)
                                                                                           GCGCGAGAAATACCGCCCAAAACAGATAAAAAAAATGTGATGCAAGTCACTATGCAGCACGCATTGAAACTATTCTGGCGGCCAAAAGCCATTG   <  SRR4302535.210268/94‑1 (MQ=60)
                                                                                            CGCGAGAAATACCGCCCAAAACAGATAAAAAAAATGTGATGCAAGTCACTATGCAGCACGCATTGAAACTATTCTGGCGGCCAAAAGCCATTGA  >  SRR4302535.4094579/1‑94 (MQ=60)
                                                                                            |                                                                                             
AAATCCCAGTGAGCAAATTCAAATAAGAATGATAACTCAGGAAAAAATTGCTCAACTTCCTAAAGACAGAGAAACGCTCTTCTATTTTAACGTGCGAGAAATACCGCCCAAAACAGATAAAAAAAATGTGATGCAAGTCACTATGCAGCACGCATTGAAACTATTCTGGCGGCCAAAAGCCATTGA  >  NC_000913/3188145‑3188330

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑