Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 1873 183.1 9376148 98.2% 9207377 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,621,415 T→C intergenic (+2/‑594) yhhH → / → yhhI hypothetical protein/putative receptor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,621,4150TC99.5% 588.0 / ‑4.9 184intergenic (+2/‑594)yhhH/yhhIhypothetical protein/putative receptor
Reads supporting (aligned to +/- strand):  ref base T (0/1);  new base C (76/107);  total (76/108)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.41e-01

Only 100 of 184 total aligned reads displayed.

TTCACTCAGACCAAATTGCTGGTGGGGTTTTTATTATAGAAATAGGCAAAAATGATGGGAGAATTCTGAATTTTGGCCACGGAAAATAAGTATGTAAGGATAATCAAACATCGTGTGCGCTGATGGCAGAGTAGGTGGAGGACTCCAGACAGTCAAACGATAGAAAAAGATAGCCTTTAT  >  NC_000913/3621325‑3621504
                                                                                          |                                                                                         
ttCACTCAGACCAAATTGCTGGTGGGGTTTTTATTATAGAAATAGGCAAAAATGATGGGAGAATTCTGAATTTTGGCCACGGAAAATAAGCAt                                                                                         <  2:4076142/93‑1 (MQ=255)
ttCACTCAGACCAAATTGCTGGTGGGGTTTTTATTATAGAAATAGGCAAAAATGATGGGAGAATTCTGAATTTTGGCCACGGAAAATAAGCAt                                                                                         <  2:2753991/93‑1 (MQ=255)
   aCTCAGACCAAATTGCTGGTGGGGTTTTTATTATAGAAATAGGCAAAAATGATGGGAGAATTCTGAATTTTGGCCACGGAAAATAAGCATGTa                                                                                      <  1:1519252/93‑1 (MQ=255)
     tCAGACCAAATTGCTGGTGGGGTTTTTATTATAGAAATAGGCAAAAATGATGGGAGAATTCTGAATTTTGGCCACGGAAAATAAGCATGTAAg                                                                                    >  1:3291472/1‑93 (MQ=255)
        gACCAAATTGCTGGTGGGGTTTTTATTATAGAAATAGGCAAAAATGATGGGAGAATTCTGAATTTTGGCCACGGAAAATAAGCATGTAAGGAt                                                                                 <  2:4663913/93‑1 (MQ=255)
        gACCAAATTGCTGGTGGGGTTTTTATTATAGAAATAGGCAAAAATGATGGGAGAATTCTGAATTTTGGCCACGGAAAATAAGCATGTAAGGAt                                                                                 >  1:1971040/1‑93 (MQ=255)
          ccAAATTGCTGGTGGGGTTTTTATTATAGAAATAGGCAAAAATGATGGGAGAATTCTGAATTTTGGCCACGGAAAATAAGCATGTAAGGATaa                                                                               <  2:4141182/93‑1 (MQ=255)
           cAAATTGCTGGTGGGGTTTTTATTATAGAAATAGGCAAAAATGATGGGAGAATTCTGAATTTTGGCCACGGAAAATAAGCATGTAAGGATAAt                                                                              <  2:3587659/93‑1 (MQ=255)
            aaaTTGCTGGTGGGGTTTTTATTATAGAAATAGGCAAAAATGATGGGAGAATTCTGAATTTTGGCCACGGAAAATAAGCATGTAAGGATAATc                                                                             <  1:4433072/93‑1 (MQ=255)
            aaaTTGCTGGTGGGGTTTTTATTATAGAAATAGGCAAAAATGATGGGAGAATTCTGAATTTTGGCCACGGAAAATAAGCATGTAAGGATAATc                                                                             >  1:3141688/1‑93 (MQ=255)
             aaTTGCTGGTGGGGTTTTTATTATAGAAATAGGCAAAAATGATGGGAGAATTCTGAATTTTGGCCACGGAAAATAAGCATGTAAGGATAATCa                                                                            >  1:2929179/1‑93 (MQ=255)
               ttGCTGGTGGGGTTTTTATTATAGAAATAGGCAAAAATGATGGGAGAATTCTGAATTTTGGCCACGGAAAATAAGCATGTAAGGATAATCaaa                                                                          <  1:1262088/93‑1 (MQ=255)
                tGCTGGTGGGGTTTTTATTATAGAAATAGGCAAAAATGATGGGAGAATTCTGAATTTTGGCCACGGAAAATAAGCATGTAAGGATAATCAAAc                                                                         <  1:1901466/93‑1 (MQ=255)
                 gCTGGTGGGGTTTTTATTATAGAAATAGGCAAAAATGATGGGAGAATTCTGAATTTTGGCCACGGAAAATAAGCATGTAAGGATAATCAAACa                                                                        <  2:2553014/93‑1 (MQ=255)
                 gCTGGTGGGGTTTTTATTATAGAAATAGGCAAAAATGATGGGAGAATTCTGAATTTTGGCCACGGAAAATAAGCATGTAAGGATAATCAAACa                                                                        <  1:2478128/93‑1 (MQ=255)
                   tggtggGGTTTTTATTATAGAAATAGGCAAAAATGATGGGAGAATTCTGAATTTTGGCCACGGAAAATAAGCATGTAAGGATAATCAAACATc                                                                      >  2:850795/1‑93 (MQ=255)
                    ggtggGGTTTTTATTATAGAAATAGGCAAAAATGATGGGAGAATTCTGAATTTTGGCCACGGAAAATAAGCATGTAAGGATAATCAAACATCg                                                                     >  1:3713260/1‑93 (MQ=255)
                      tggGGTTTTTATTATAGAAATAGGCAAAAATGATGGGAGAATTCTGAATTTTGGCCACGGAAAATAAGCATGTAAGGATAATCAAACATCgtg                                                                   >  1:3258238/1‑93 (MQ=255)
                      tggGGTTTTTATTATAGAAATAGGCAAAAATGATGGGAGAATTCTGAATTTTGGCCACGGAAAATAAGCATGTAAGGATAATCAAACATCgtg                                                                   >  2:3966416/1‑93 (MQ=255)
                       ggggTTTTTATTATAGAAATAGGCAAAAATGATGGGAGAATTCTGAATTTTGGCCACGGAAAATAAGCATGTAAGGATAATCAAACATCgtgt                                                                  <  2:7981/93‑1 (MQ=255)
                       ggggTTTTTATTATAGAAATAGGCAAAAATGATGGGAGAATTCTGAATTTTGGCCACGGAAAATAAGCATGTAAGGATAATCAAACATCgtgt                                                                  <  1:3562365/93‑1 (MQ=255)
                       ggggTTTTTATTATAGAAATAGGCAAAAATGATGGGAGAATTCTGAATTTTGGCCACGGAAAATAAGCATGTAAGGATAATCAAACATCgtgt                                                                  >  1:3796916/1‑93 (MQ=255)
                          gTTTTTATTATAGAAATAGGCAAAAATGATGGGAGAATTCTGAATTTTGGCCACGGAAAATAAGCATGTAAGGATAATCAAACATCGTGTgcg                                                               >  1:17104/1‑93 (MQ=255)
                           tttttATTATAGAAATAGGCAAAAATGATGGGAGAATTCTGAATTTTGGCCACGGAAAATAAGCATGTAAGGATAATCAAACATCGTGTgcgc                                                              >  2:2178132/1‑93 (MQ=255)
                             tttATTATAGAAATAGGCAAAAATGATGGGAGAATTCTGAATTTTGGCCACGGAAAATAAGCATGTAAGGATAATCAAACATCGTGTGCGCTg                                                            >  1:3958515/1‑93 (MQ=255)
                              ttattaTAGAAATAGGCAAAAATGATGGGAGAATTCTGAATTTTGGCCACGGAAAATAAGCATGTAAGGATAATCAAACATCGTGTGCGCTGa                                                           >  1:4676940/1‑93 (MQ=255)
                               tattatAGAAATAGGCAAAAATGATGGGAGAATTCTGAATTTTGGCCACGGAAAATAAGCATGTAAGGATAATCAAACATCGTGTGCGCTGAt                                                          <  1:73780/93‑1 (MQ=255)
                                attatAGAAATAGGCAAAAATGATGGGAGAATTCTGAATTTTGGCCACGGAAAATAAGCATGTAAGGATAATCAAACATCGTGTGCGCTGATg                                                         <  2:2303590/93‑1 (MQ=255)
                                  tataGAAATAGGCAAAAATGATGGGAGAATTCTGAATTTTGGCCACGGAAAATAAGCATGTAAGGATAATCAAACATCGTGTGCGCTGATGGc                                                       <  2:531896/93‑1 (MQ=255)
                                  tataGAAATAGGCAAAAATGATGGGAGAATTCTGAATTTTGGCCACGGAAAATAAGCATGTAAGGATAATCAAACATCGTGTGCGCTGATGGc                                                       >  1:4229067/1‑93 (MQ=255)
                                      gAAATAGGCAAAAATGATGGGAGAATTCTGAATTTTGGCCACGGAAAATAAGCATGTAAGGATAATCAAACATCGTGTGCGCTGATGGCagag                                                   <  1:4474547/93‑1 (MQ=255)
                                      gAAATAGGCAAAAATGATGGGAGAATTCTGAATTTTGGCCACGGAAAATAAGCATGTAAGGATAATCAAACATCGTGTGCGCTGATGGCagag                                                   >  2:1037812/1‑93 (MQ=255)
                                       aaaTAGGCAAAAATGATGGGAGAATTCTGAATTTTGGCCACGGAAAATAAGCATGTAAGGATAATCAAACATCGTGTGCGCTGATGGCAGAGt                                                  <  2:206905/93‑1 (MQ=255)
                                        aaTAGGCAAAAATGATGGGAGAATTCTGAATTTTGGCCACGGAAAATAAGCATGTAAGGATAATCAAACATCGTGTGCGCTGATGGCAGAGTa                                                 >  2:1157923/1‑93 (MQ=255)
                                        aaTAGGCAAAAATGATGGGAGAATTCTGAATTTTGGCCACGGAAAATAAGCATGTAAGGATAATCAAACATCGTGTGCGCTGATGGCAGAGTa                                                 <  1:2409881/93‑1 (MQ=255)
                                        aaTAGGCAAAAATGATGGGAGAATTCTGAATTTTGGCCACGGAAAATAAGCATGTAAGGATAATCAAACATCGTGTGCGCTGATGGCAGAGTa                                                 <  2:1944588/93‑1 (MQ=255)
                                           aGGCAAAAATGATGGGAGAATTCTGAATTTTGGCCACGGAAAATAAGCATGTAAGGATAATCAAACATCGTGTGCGCTGATGGCAGAGTAGGt                                              <  2:3952636/93‑1 (MQ=255)
                                           aGGCAAAAATGATGGGAGAATTCTGAATTTTGGCCACGGAAAATAAGCATGTAAGGATAATCAAACATCGTGTGCGCTGATGGCAGAGTAGGt                                              >  1:336037/1‑93 (MQ=255)
                                           aGGCAAAAATGATGGGAGAATTCTGAATTTTGGCCACGGAAAATAAGCATGTAAGGATAATCAAACATCGTGTGCGCTGATGGCAGAGTAGGt                                              <  1:3347603/93‑1 (MQ=255)
                                            ggCAAAAATGATGGGAGAATTCTGAATTTTGGCCACGGAAAATAAGCATGTAAGGATAATCAAACATCGTGTGCGCTGATGGCAGAGTAGGTg                                             <  2:3707420/93‑1 (MQ=255)
                                            ggCAAAAATGATGGGAGAATTCTGAATTTTGGCCACGGAAAATAAGCATGTAAGGATAATCAAACATCGTGTGCGCTGATGGCAGAGTAGGTg                                             >  1:2221076/1‑93 (MQ=255)
                                              cAAAAATGATGGGAGAATTCTGAATTTTGGCCACGGAAAATAAGCATGTAAGGATAATCAAACATCGTGTGCGCTGATGGCAGAGTAGGTgga                                           <  1:1542535/93‑1 (MQ=255)
                                              cAAAAATGATGGGAGAATTCTGAATTTTGGCCACGGAAAATAAGCATGTAAGGATAATCAAACATCGTGTGCGCTGATGGCAGAGTAGGTgga                                           <  2:1862389/93‑1 (MQ=255)
                                              cAAAAATGATGGGAGAATTCTGAATTTTGGCCACGGAAAATAAGCATGTAAGGATAATCAAACATCGTGTGCGCTGATGGCAGAGTAGGTgga                                           <  2:2877470/93‑1 (MQ=255)
                                               aaaaaTGATGGGAGAATTCTGAATTTTGGCCACGGAAAATAAGCATGTAAGGATAATCAAACATCGTGTGCGCTGATGGCAGAGTAGGTggag                                          <  1:2186951/93‑1 (MQ=255)
                                                aaaaTGATGGGAGAATTCTGAATTTTGGCCACGGAAAATAAGCATGTAAGGATAATCAAACATCGTGTGCGCTGATGGCAGAGTAGGTggagg                                         >  1:176180/1‑93 (MQ=255)
                                                aaaaTGATGGGAGAATTCTGAATTTTGGCCACGGAAAATAAGCATGTAAGGATAATCAAACATCGTGTGCGCTGATGGCAGAGTAGGTggagg                                         >  2:2647309/1‑93 (MQ=255)
                                                aaaaTGATGGGAGAATTCTGAATTTTGGCCACGGAAAATAAGCATGTAAGGATAATCAAACATCGTGTGCGCTGATGGCAGAGTAGGTggagg                                         >  1:2845979/1‑93 (MQ=255)
                                                aaaaTGATGGGAGAATTCTGAATTTTGGCCACGGAAAATAAGCATGTAAGGATAATCAAACATCGTGTGCGCTGATGGCAGAGTAGGGggagg                                         >  2:190126/1‑93 (MQ=255)
                                                 aaaTGATGGGAGAATTCTGAATTTTGGCCACGGAAAATAAGCATGTAAGGATAATCAAACATCGTGTGCGCTGATGGCAGAGTAGGTggagga                                        >  2:3286428/1‑93 (MQ=255)
                                                 aaaTGATGGGAGAATTCTGAATTTTGGCCACGGAAAATAAGCATGTAAGGATAATCAAACATCGTGTGCGCTGATGGCAGAGTAGGTggagga                                        <  2:2852895/93‑1 (MQ=255)
                                                  aaTGATGGGAGAATTCTGAATTTTGGCCACGGAAAATAAGCATGTAAGGATAATCAAACATCGTGTGCGCTGATGGCAGAGTAGGTGGAGGAc                                       <  2:2527003/93‑1 (MQ=255)
                                                  aaTGATGGGAGAATTCTGAATTTTGGCCACGGAAAATAAGCATGTAAGGATAATCAAACATCGTGTGCGCTGATGGCAGAGTAGGTGGAGGAc                                       <  1:1124413/93‑1 (MQ=255)
                                                   atgatgGGAGAATTCTGAATTTTGGCCACGGAAAATAAGCATGTAAGGATAATCAAACATCGTGTGCGCTGATGGCAGAGTAGGTGGAGGACt                                      >  2:1258976/1‑93 (MQ=255)
                                                    tgatgGGAGAATTCTGAATTTTGGCCACGGAAAATAAGCATGTAAGGATAATCAAACATCGTGTGCGCTGATGGCAGAGTAGGTGGAGGACTc                                     >  1:3531529/1‑93 (MQ=255)
                                                     gatgGGAGAATTCTGAATTTTGGCCACGGAAAATAAGCATGTAAGGATAATCAAACATCGTGTGCGCTGATGGCAGAGTAGGTGGAGGACTcc                                    <  2:634810/93‑1 (MQ=255)
                                                       tgGGAGAATTCTGAATTTTGGCCACGGAAAATAAGCATGTAAGGATAATCAAACATCGTGTGCGCTGATGGCAGAGTAGGTGGAGGACTCCAg                                  >  2:479267/1‑93 (MQ=255)
                                                         ggAGAATTCTGAATTTTGGCCACGGAAAATAAGCATGTAAGGATAATCAAACATCGTGTGCGCTGATGGCAGAGTAGGTGGAGGACTCCAGAc                                <  2:1337489/93‑1 (MQ=255)
                                                         ggAGAATTCTGAATTTTGGCCACGGAAAATAAGCATGTAAGGATAATCAAACATCGTGTGCGCTGATGGCAGAGTAGGTGGAGGACTCCAGAc                                >  1:1294803/1‑93 (MQ=255)
                                                          gagaATTCTGAATTTTGGCCACGGAAAATAAGCATGTAAGGATAATCAAACATCGTGTGCGCTGATGGCAGAGTAGGTGGAGGACTCCAGACa                               <  2:641062/93‑1 (MQ=255)
                                                          gagaATTCTGAATTTTGGCCACGGAAAATAAGCATGTAAGGATAATCAAACATCGTGTGCGCTGATGGCAGAGTAGGTGGAGGACTCCAGACa                               <  2:2312991/93‑1 (MQ=255)
                                                          gagaATTCTGAATTTTGGCCACGGAAAATAAGCATGTAAGGATAATCAAACATCGTGTGCGCTGATGGCAGAGTAGGTGGAGGACTCCAGACa                               <  2:2144936/93‑1 (MQ=255)
                                                          gagaATTCTGAATTTTGGCCACGGAAAATAAGCATGTAAGGATAATCAAACATCGTGTGCGCTGATGGCAGAGTAGGTGGAGGACTCCAGACa                               >  1:3071967/1‑93 (MQ=255)
                                                           agaATTCTGAATTTTGGCCACGGAAAATAAGCATGTAAGGATAATCAAACATCGTGTGCGCTGATGGCAGAGTAGGTGGAGGACTCCAGACAg                              <  2:4021242/93‑1 (MQ=255)
                                                            gaATTCTGAATTTTGGCCACGGAAAATAAGCATGTAAGGATAATCAAACATCGTGTGCGCTGATGGCAGAGTAGGTGGAGGACTCCAGACAGt                             >  1:3005328/1‑93 (MQ=255)
                                                            gaATTCTGAATTTTGGCCACGGAAAATAAGCATGTAAGGATAATCAAACATCGTGTGCGCTGATGGCAGAGTAGGTGGAGGACTCCAGACAGt                             >  2:3305724/1‑93 (MQ=255)
                                                             aaTTCTGAATTTTGGCCACGGAAAATAAGCATGTAAGGATAATCAAACATCGTGTGCGCTGATGGCAGAGTAGGTGGAGGACTACAGACAGTc                            >  2:2933480/1‑93 (MQ=255)
                                                               ttCTGAATTTTGGCCACGGAAAATAAGCATGTAAGGATAATCAAACATCGTGTGCGCTGATGGCAGAGTAGGTGGAGGACTCCAGACAGTCaa                          >  2:4532100/1‑93 (MQ=255)
                                                                tCTGAATTTTGGCCACGGAAAATAAGCATGTAAGGATAATCAAACATCGTGTGCGCTGATGGCAGAGTAGGTGGAGGACTCCAGACAGTCaaa                         <  1:1934694/93‑1 (MQ=255)
                                                                tCTGAATTTTGGCCACGGAAAATAAGCATGTAAGGATAATCAAACATCGTGTGCGCTGATGGCAGAGTAGGTGGAGGACTCCAGACAGTCaaa                         >  1:1040466/1‑93 (MQ=255)
                                                                tCTGAATTTTGGCCACGGAAAATAAGCATGTAAGGATAATCAAACATCGTGTGCGCTGATGGCAGAGTAGGTGGAGGACTCCAGACAGTCaaa                         <  1:3623166/93‑1 (MQ=255)
                                                                tCTGAATTTTGGCCACGGAAAATAAGCATGTAAGGATAATCAAACATCGTGTGCGCTGATGGCAGAGTAGGTGGAGGACTCCAGACAGTCaaa                         >  1:2572872/1‑93 (MQ=255)
                                                                 cTGAATTTTGGCCACGGAAAATAAGCATGTAAGGATAATCAAACATCGTGTGCGCTGATGGCAGAGTAGGTGGAGGACTCCAGACAGTCAAAc                        <  2:3796916/93‑1 (MQ=255)
                                                                   gAATTTTGGCCACGGAAAATAAGCATGTAAGGATAATCAAACATCGTGTGCGCTGATGGCAGAGTAGGTGGAGGACTCCAGACAGTCAAACGa                      <  1:2535765/93‑1 (MQ=255)
                                                                   gAATTTTGGCCACGGAAAATAAGCATGTAAGGATAATCAAACATCGTGTGCGCTGATGGCAGAGTAGGTGGAGGACTACAGACAGTCAAACGa                      >  1:1300351/1‑93 (MQ=255)
                                                                     aTTTTGGCCACGGAAAATAAGCATGTAAGGATAATCAAACATCGTGTGCGCTGATGGCAGAGTAGGTGGAGGACTCCAGACAGTCAAACGATa                    <  1:2915509/93‑1 (MQ=255)
                                                                     aTTTTGGCCACGGAAAATAAGCATGTAAGGATAATCAAACATCGTGTGCGCTGATGGCAGAGTAGGTGGAGGACTCCAGACAGTCAAACGATa                    <  1:1838633/93‑1 (MQ=255)
                                                                     aTTTTGGCCACGGAAAATAAGCATGTAAGGATAATCAAACATCGTGTGCGCTGATGGCAGAGTAGGTGGAGGACTCCAGACAGTCAAACGATa                    <  1:2041734/93‑1 (MQ=255)
                                                                     aTTTTGGCCACGGAAAATAAGCATGTAAGGATAATCAAACATCGTGTGCGCTGATGGCAGAGTAGGTGGAGGACTCCAGACAGTCAAACGATa                    <  2:3403050/93‑1 (MQ=255)
                                                                      ttttGGCCACGGAAAATAAGCATGTAAGGATAATCAAACATCGTGTGCGCTGATGGCAGAGTAGGTGGAGGACTCCAGACAGTCAAACGATAg                   >  2:4290936/1‑93 (MQ=255)
                                                                       tttGGCCACGGAAAATAAGCATGTAAGGATAATCAAACATCGTGTGCGCTGATGGTAGAGTAGGTGGAGGACTCCAGACAGTCAAACGATAGa                  <  2:1829508/93‑1 (MQ=255)
                                                                         tGGCCACGGAAAATAAGCATGTAAGGATAATCAAACATCGTGTGCGCTGATGGCAGAGTAGGTGGAGGACTCCAGACAGTCAAACGATAGaaa                <  2:4352012/93‑1 (MQ=255)
                                                                         tGGCCACGGAAAATAAGCATGTAAGGATAATCAAACATCGTGTGCGCTGATGGCAGAGTAGGTGGAGGACTCCAGACAGTCAAACGATAGaaa                <  1:186460/93‑1 (MQ=255)
                                                                         tGGCCACGGAAAATAAGCATGTAAGGATAATCAAACATCGTGTGCGCTGATGGCAGAGTAGGTGGAGGACTCCAGACAGTCAAACGATAGaaa                <  1:251374/93‑1 (MQ=255)
                                                                           gCCACGGAAAATAAGCATGTAAGGATAATCAAACATCGTGTGCGCTGATGGCAGAGTAGGTGGAGGACTCCAGACAGTCAAACGATAGaaaaa              <  1:4019582/93‑1 (MQ=255)
                                                                            ccACGGAAAATAAGCATGTAAGGATAATCAAACATCGTGTGCGCTGATGGCAGAGTAGGTGGAGGACTCCAGACAGTCAAACGATAGAAAAAg             <  2:2709230/93‑1 (MQ=255)
                                                                             cACGGAAAATAAGCATGTAAGGATAATCAAACATCGTGTGCGCTGATGGCAGAGTAGGTGGAGGACTCCAGACAGTCAAACGATAGAAAAAGa            >  1:135302/1‑93 (MQ=255)
                                                                              aCGGAAAATAAGCATGTAAGGATAATCAAACATCGTGTGCGCTGATGGCAGAGTAGGTGGAGGACTCCAGACAGTCAAACGATAGAAAAAGAt           >  1:4319483/1‑93 (MQ=255)
                                                                              aCGGAAAATAAGCATGTAAGGATAATCAAACATCGTGTGCGCTGATGGCAGAGTAGGTGGAGGACTCCAGACAGTCAAACGATAGAAAAAGAt           <  1:1031963/93‑1 (MQ=255)
                                                                              aCGGAAAATAAGCATGTAAGGATAATCAAACATCGTGTGCGCTGATGGCAGAGTAGGTGGAGGACTCCAGACAGTCAAACGATAGAAAAAGAt           <  1:3222469/93‑1 (MQ=255)
                                                                               cGGAAAATAAGCATGTAAGGATAATCAAACATCGTGTGCGCTGATGGCAGAGTAGGTGGAGGACTCCAGACAGTCAAACGATAGAAAAAGATa          <  1:2961466/93‑1 (MQ=255)
                                                                                ggAAAATAAGCATGTAAGGATAATCAAACATCGTGTGCGCTGATGGCAGAGTAGGTGGAGGACTCCAGACAGTCAAACGATAGAAAAAGATAg         >  1:4168235/1‑93 (MQ=255)
                                                                                   aaaTAAGCATGTAAGGATAATCAAACATCGTGTGCGCTGATGGCAGAGTAGGTGGAGGACTCCAGACAGTCAAACGATAGAAAAAGATAGCCt      <  2:3643457/93‑1 (MQ=255)
                                                                                   aaaTAAGCATGTAAGGATAATCAAACATCGTGTGCGCTGATGGCAGAGTAGGTGGAGGACTCCAGACAGTCAAACGATAGAAAAAGATAGCCt      <  1:966343/93‑1 (MQ=255)
                                                                                   aaaTAAGCATGTAAGGATAATCAAACATCGTGTGCGCTGATGGCAGAGTAGGTGGAGGACTCCAGACAGTCAAACGATAGAAAAAGATAGCCt      <  1:134147/93‑1 (MQ=255)
                                                                                    aaTAAGCATGTAAGGATAATCAAACATCGTGTGCGCTGATGGCAGAGTAGGTGGAGGACTCCAGACAGTCAAACGATAGAAAAAGATAGCCtt     >  1:2671460/1‑93 (MQ=255)
                                                                                    aaTAAGCATGTAAGGATAATCAAACATCGTGTGCGCTGATGGCAGAGTAGGTGGAGGACTCCAGACAGTCAAACGATAGAAAAAGATAGCCtt     >  2:2005287/1‑93 (MQ=255)
                                                                                      tAAGCATGTAAGGATAATCAAACATCGTGTGCGCTGATGGCAGAGTAGGTGGAGGACTCCAGACAGTCAAACGATAGAAAAAGATAGCCTTTa   <  1:1452869/93‑1 (MQ=255)
                                                                                      tAAGCATGTAAGGATAATCAAACATCGTGTGCGCTGATGGCAGAGTAGGTGGAGGACTCCAGACAGTCAAACGATAGAAAAAGATAGCCTTTa   <  2:1955642/93‑1 (MQ=255)
                                                                                      tAAGCATGTAAGGATAATCAAACATCGTGTGCGCTGATGGCAGAGTAGGTGGAGGACTCCAGACAGTCAAACGATAGAAAAAGATAGCCTTTa   <  1:2061546/93‑1 (MQ=255)
                                                                                          |                                                                                         
TTCACTCAGACCAAATTGCTGGTGGGGTTTTTATTATAGAAATAGGCAAAAATGATGGGAGAATTCTGAATTTTGGCCACGGAAAATAAGTATGTAAGGATAATCAAACATCGTGTGCGCTGATGGCAGAGTAGGTGGAGGACTCCAGACAGTCAAACGATAGAAAAAGATAGCCTTTAT  >  NC_000913/3621325‑3621504

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
ATTCACTCAGACCAAATTGCTGGTGGGGTTTTTATTATAGAAATAGGCAAAAATGATGGGAGAATTCTGAATTTTGGCCACGGAAAATAAGTATGTAAGGATAATCAAACATCGTGTGCGCTGATGGCAGAGTAGGTGGAGGACTCCAGACAGTCAAACGATAGAAAAAGATAGCCTTTATGGA  >  NC_000913/3621324‑3621507
                                                                                           |                                                                                            
ATTCACTCAGACCAAATTGCTGGTGGGGTTTTTATTATAGAAATAGGCAAAAATGATGGGAGAATTCTGAATTTTGGCCACGGAAAATAAGCAT                                                                                            >  SRR4302535.3095945/1‑94 (MQ=60)
    ACTCAGACCAAATTGCTGGTGGGGTTTTTATTATAGAAATAGGCAAAAATGATGGGAGAATTCTGAATTTTGGCCACGGAAAATAAGCATGTAA                                                                                        <  SRR4302535.1530194/94‑1 (MQ=60)
     CTCAGACCAAATTGCTGGTGGGGTTTTTATTATAGAAATAGGCAAAAATGATGGGAGAATTCTGAATTTTGGCCACGGAAAATAAGCATGTAAG                                                                                       >  SRR4302535.3314360/1‑94 (MQ=60)
       CAGACCAAATTGCTGGTGGGGTTTTTATTATAGAAATAGGCAAAAATGATGGGAGAATTCTGAATTTTGGCCACGGAAAATAAGCATGTAAGGA                                                                                     <  SRR4302535.4604104/94‑1 (MQ=60)
        AGACCAAATTGCTGGTGGGGTTTTTATTATAGAAATAGGCAAAAATGATGGGAGAATTCTGAATTTTGGCCACGGAAAATAAGCATGTAAGGAT                                                                                    >  SRR4302535.1985236/1‑94 (MQ=60)
         GACCAAATTGCTGGTGGGGTTTTTATTATAGAAATAGGCAAAAATGATGGGAGAATTCTGAATTTTGGCCACGGAAAATAAGCATGTAAGGATA                                                                                   <  SRR4302535.2599121/94‑1 (MQ=60)
            CAAATTGCTGGTGGGGTTTTTATTATAGAAATAGGCAAAAATGATGGGAGAATTCTGAATTTTGGCCACGGAAAATAAGCATGTAAGGATAATC                                                                                >  SRR4302535.3163548/1‑94 (MQ=60)
             AAATTGCTGGTGGGGTTTTTATTATAGAAATAGGCAAAAATGATGGGAGAATTCTGAATTTTGGCCACGGAAAATAAGCATGTAAGGATAATCA                                                                               <  SRR4302535.3039663/94‑1 (MQ=60)
             AAATTGCTGGTGGGGTTTTTATTATAGAAATAGGCAAAAATGATGGGAGAATTCTGAATTTTGGCCACGGAAAATAAGCATGTAAGGATAATCA                                                                               <  SRR4302535.4463448/94‑1 (MQ=60)
             AAATTGCTGGTGGGGTTTTTATTATAGAAATAGGCAAAAATGATGGGAGAATTCTGAATTTTGGCCACGGAAAATAAGCATGTAAGGATAATCA                                                                               >  SRR4302535.2949818/1‑94 (MQ=60)
                TTGCTGGTGGGGTTTTTATTATAGAAATAGGCAAAAATGATGGGAGAATTCTGAATTTTGGCCACGGAAAATAAGCATGTAAGGATAATCAAAC                                                                            <  SRR4302535.1271120/94‑1 (MQ=60)
                 TGCTGGTGGGGTTTTTATTATAGAAATAGGCAAAAATGATGGGAGAATTCTGAATTTTGGCCACGGAAAATAAGCATGTAAGGATAATCAAACA                                                                           <  SRR4302535.1915151/94‑1 (MQ=60)
                  GCTGGTGGGGTTTTTATTATAGAAATAGGCAAAAATGATGGGAGAATTCTGAATTTTGGCCACGGAAAATAAGCATGTAAGGATAATCAAACAT                                                                          <  SRR4302535.2928869/94‑1 (MQ=60)
                  GCTGGTGGGGTTTTTATTATAGAAATAGGCAAAAATGATGGGAGAATTCTGAATTTTGGCCACGGAAAATAAGCATGTAAGGATAATCAAACAT                                                                          <  SRR4302535.2496001/94‑1 (MQ=60)
                   CTGGTGGGGTTTTTATTATAGAAATAGGCAAAAATGATGGGAGAATTCTGAATTTTGGCCACGGAAAATAAGCATGTAAGGATAATCAAACATC                                                                         <  SRR4302535.1503533/94‑1 (MQ=60)
                   CTGGTGGGGTTTTTATTATAGAAATAGGCAAAAATGATGGGAGAATTCTGAATTTTGGCCACGGAAAATAAGCATGTAAGGATAATCAAACATC                                                                         <  SRR4302535.1530407/94‑1 (MQ=60)
                    TGGTGGGGTTTTTATTATAGAAATAGGCAAAAATGATGGGAGAATTCTGAATTTTGGCCACGGAAAATAAGCATGTAAGGATAATCAAACATCG                                                                        >  SRR4302535.3738986/1‑94 (MQ=60)
                     GGTGGGGTTTTTATTATAGAAATAGGCAAAAATGATGGGAGAATTCTGAATTTTGGCCACGGAAAATAAGCATGTAAGGATAATCAAACATCGT                                                                       <  SRR4302535.1868912/94‑1 (MQ=60)
                      GTGGGGTTTTTATTATAGAAATAGGCAAAAATGATGGGAGAATTCTGAATTTTGGCCACGGAAAATAAGCATGTAAGGATAATCAAACATCGTG                                                                      >  SRR4302535.3280881/1‑94 (MQ=60)
                       TGGGGTTTTTATTATAGAAATAGGCAAAAATGATGGGAGAATTCTGAATTTTGGCCACGGAAAATAAGCATGTAAGGATAATCAAACATCGTGT                                                                     >  SRR4302535.3823091/1‑94 (MQ=60)
                        GGGGTTTTTATTATAGAAATAGGCAAAAATGATGGGAGAATTCTGAATTTTGGCCACGGAAAATAAGCATGTAAGGATAATCAAACATCGTGTG                                                                    <  SRR4302535.3587065/94‑1 (MQ=60)
                          GGTTTTTATTATAGAAATAGGCAAAAATGATGGGAGAATTCTGAATTTTGGCCACGGAAAATAAGCATGTAAGGATAATCAAACATCGTGTGCG                                                                  >  SRR4302535.17231/1‑94 (MQ=60)
                            TTTTTATTATAGAAATAGGCAAAAATGATGGGAGAATTCTGAATTTTGGCCACGGAAAATAAGCATGTAAGGATAATCAAACATCGTGTGCGCT                                                                <  SRR4302535.2098889/94‑1 (MQ=60)
                             TTTTATTATAGAAATAGGCAAAAATGATGGGAGAATTCTGAATTTTGGCCACGGAAAATAAGCATGTAAGGATAATCAAACATCGTGTGCGCTG                                                               >  SRR4302535.3985546/1‑94 (MQ=60)
                              TTTATTATAGAAATAGGCAAAAATGATGGGAGAATTCTGAATTTTGGCCACGGAAAATAAGCATGTAAGGATAATCAAACATCGTGTGCGCTGA                                                              >  SRR4302535.4708506/1‑94 (MQ=60)
                              TTTATTATAGAAATAGGCAAAAATGATGGGAGAATTCTGAATTTTGGCCACGGAAAATAAGCATGTAAGGATAATCAAACATCGTGTGCGCTGA                                                              <  SRR4302535.1941677/94‑1 (MQ=60)
                               TTATTATAGAAATAGGCAAAAATGATGGGAGAATTCTGAATTTTGGCCACGGAAAATAAGCATGTAAGGATAATCAAACATCGTGTGCGCTGAT                                                             >  SRR4302535.1313171/1‑94 (MQ=60)
                                TATTATAGAAATAGGCAAAAATGATGGGAGAATTCTGAATTTTGGCCACGGAAAATAAGCATGTAAGGATAATCAAACATCGTGTGCGCTGATG                                                            <  SRR4302535.74315/94‑1 (MQ=60)
                                 ATTATAGAAATAGGCAAAAATGATGGGAGAATTCTGAATTTTGGCCACGGAAAATAAGCATGTAAGGATAATCAAACATCGTGTGCGCTGATGG                                                           <  SRR4302535.3970376/94‑1 (MQ=60)
                                  TTATAGAAATAGGCAAAAATGATGGGAGAATTCTGAATTTTGGCCACGGAAAATAAGCATGTAAGGATAATCAAACATCGTGTGCGCTGATGGC                                                          >  SRR4302535.4258011/1‑94 (MQ=60)
                                  TTATAGAAATAGGCAAAAATGATGGGAGAATTCTGAATTTTGGCCACGGAAAATAAGCATGTAAGGATAATCAAACATCGTGTGCGCTGATGGC                                                          >  SRR4302535.2524096/1‑94 (MQ=60)
                                    ATAGAAATAGGCAAAAATGATGGGAGAATTCTGAATTTTGGCCACGGAAAATAAGCATGTAAGGATAATCAAACATCGTGTGCGCTGATGGCAG                                                        >  SRR4302535.2543359/1‑94 (MQ=60)
                                     TAGAAATAGGCAAAAATGATGGGAGAATTCTGAATTTTGGCCACGGAAAATAAGCATGTAAGGATAATCAAACATCGTGTGCGCTGATGGCAGA                                                       <  SRR4302535.1059372/94‑1 (MQ=60)
                                       GAAATAGGCAAAAATGATGGGAGAATTCTGAATTTTGGCCACGGAAAATAAGCATGTAAGGATAATCAAACATCGTGTGCGCTGATGGCAGAGT                                                     <  SRR4302535.4505248/94‑1 (MQ=60)
                                       GAAATAGGCAAAAATGATGGGAGAATTCTGAATTTTGGCCACGGAAAATAAGCATGTAAGGATAATCAAACATCGTGTGCGCTGATGGCAGAGT                                                     <  SRR4302535.4282430/94‑1 (MQ=60)
                                        AAATAGGCAAAAATGATGGGAGAATTCTGAATTTTGGCCACGGAAAATAAGCATGTAAGGATAATCAAACATCGTGTGCGCTGATGGCAGAGTA                                                    >  SRR4302535.3614763/1‑94 (MQ=60)
                                         AATAGGCAAAAATGATGGGAGAATTCTGAATTTTGGCCACGGAAAATAAGCATGTAAGGATAATCAAACATCGTGTGCGCTGATGGCAGAGTAG                                                   <  SRR4302535.2793213/94‑1 (MQ=60)
                                         AATAGGCAAAAATGATGGGAGAATTCTGAATTTTGGCCACGGAAAATAAGCATGTAAGGATAATCAAACATCGTGTGCGCTGATGGCAGAGTAG                                                   <  SRR4302535.2427231/94‑1 (MQ=60)
                                           TAGGCAAAAATGATGGGAGAATTCTGAATTTTGGCCACGGAAAATAAGCATGTAAGGATAATCAAACATCGTGTGCGCTGATGGCAGAGTAGGT                                                 >  SRR4302535.338363/1‑94 (MQ=60)
                                            AGGCAAAAATGATGGGAGAATTCTGAATTTTGGCCACGGAAAATAAGCATGTAAGGATAATCAAACATCGTGTGCGCTGATGGCAGAGTAGGTG                                                <  SRR4302535.3370923/94‑1 (MQ=60)
                                            AGGCAAAAATGATGGGAGAATTCTGAATTTTGGCCACGGAAAATAAGCATGTAAGGATAATCAAACATCGTGTGCGCTGATGGCAGAGTAGGTG                                                >  SRR4302535.2236889/1‑94 (MQ=60)
                                               CAAAAATGATGGGAGAATTCTGAATTTTGGCCACGGAAAATAAGCATGTAAGGATAATCAAACATCGTGTGCGCTGATGGCAGAGTAGGTGGAG                                             <  SRR4302535.1893035/94‑1 (MQ=60)
                                               CAAAAATGATGGGAGAATTCTGAATTTTGGCCACGGAAAATAAGCATGTAAGGATAATCAAACATCGTGTGCGCTGATGGCAGAGTAGGTGGAG                                             >  SRR4302535.144536/1‑94 (MQ=60)
                                               CAAAAATGATGGGAGAATTCTGAATTTTGGCCACGGAAAATAAGCATGTAAGGATAATCAAACATCGTGTGCGCTGATGGCAGAGTAGGTGGAG                                             <  SRR4302535.1553654/94‑1 (MQ=60)
                                                AAAAATGATGGGAGAATTCTGAATTTTGGCCACGGAAAATAAGCATGTAAGGATAATCAAACATCGTGTGCGCTGATGGCAGAGTAGGTGGAGG                                            >  SRR4302535.3570516/1‑94 (MQ=60)
                                                AAAAATGATGGGAGAATTCTGAATTTTGGCCACGGAAAATAAGCATGTAAGGATAATCAAACATCGTGTGCGCTGATGGCAGAGTAGGTGGAGG                                            <  SRR4302535.2202531/94‑1 (MQ=60)
                                                AAAAATGATGGGAGAATTCTGAATTTTGGCCACGGAAAATAAGCATGTAAGGATAATCAAACATCGTGTGCGCTGATGGCAGAGTAGGTGGAGG                                            >  SRR4302535.177405/1‑94 (MQ=60)
                                                AAAAATGATGGGAGAATTCTGAATTTTGGCCACGGAAAATAAGCATGTAAGGATAATCAAACATCGTGTGCGCTGATGGCAGAGTAGGTGGAGG                                            >  SRR4302535.2866156/1‑94 (MQ=60)
                                                   AATGATGGGAGAATTCTGAATTTTGGCCACGGAAAATAAGCATGTAAGGATAATCAAACATCGTGTGCGCTGATGGCAGAGTAGGTGGAGGACT                                         <  SRR4302535.1132437/94‑1 (MQ=60)
                                                   AATGATGGGAGAATTCTGAATTTTGGCCACGGAAAATAAGCATGTAAGGATAATCAAACATCGTGTGCGCTGATGGCAGAGTAGGTGGAGGACT                                         <  SRR4302535.4076768/94‑1 (MQ=60)
                                                   AATGATGGGAGAATTCTGAATTTTGGCCACGGAAAATAAGCATGTAAGGATAATCAAACATCGTGTGCGCTGATGGCAGAGTAGGTGGAGGACT                                         >  SRR4302535.3187235/1‑94 (MQ=60)
                                                   AATGATGGGAGAATTCTGAATTTTGGCCACGGAAAATAAGCATGTAAGGATAATCAAACATCGTGTGCGCTGATGGCAGAGTAGGTGGAGGACT                                         <  SRR4302535.3247532/94‑1 (MQ=60)
                                                    ATGATGGGAGAATTCTGAATTTTGGCCACGGAAAATAAGCATGTAAGGATAATCAAACATCGTGTGCGCTGATGGCAGAGTAGGTGGAGGACTC                                        >  SRR4302535.3556046/1‑94 (MQ=60)
                                                    ATGATGGGAGAATTCTGAATTTTGGCCACGGAAAATAAGCATGTAAGGATAATCAAACATCGTGTGCGCTGATGGCAGAGTAGGTGGAGGACTC                                        >  SRR4302535.486891/1‑94 (MQ=60)
                                                      GATGGGAGAATTCTGAATTTTGGCCACGGAAAATAAGCATGTAAGGATAATCAAACATCGTGTGCGCTGATGGCAGAGTAGGTGGAGGACTCCA                                      >  SRR4302535.1765363/1‑94 (MQ=60)
                                                         GGGAGAATTCTGAATTTTGGCCACGGAAAATAAGCATGTAAGGATAATCAAACATCGTGTGCGCTGATGGCAGAGTAGGTGGAGGACTCCAGAC                                   >  SRR4302535.1304035/1‑94 (MQ=60)
                                                         GGGAGAATTCTGAATTTTGGCCACGGAAAATAAGCATGTAAGGATAATCAAACATCGTGTGCGCTGATGGCAGAGTAGGTGGAGGACTCCAGAC                                   >  SRR4302535.4360856/1‑94 (MQ=60)
                                                          GGAGAATTCTGAATTTTGGCCACGGAAAATAAGCATGTAAGGATAATCAAACATCGTGTGCGCTGATGGCAGAGTAGGTGGAGGACTCCAGACA                                  >  SRR4302535.3093414/1‑94 (MQ=60)
                                                            AGAATTCTGAATTTTGGCCACGGAAAATAAGCATGTAAGGATAATCAAACATCGTGTGCGCTGATGGCAGAGTAGGTGGAGGACTCCAGACAGT                                >  SRR4302535.3026390/1‑94 (MQ=60)
                                                               ATTCTGAATTTTGGCCACGGAAAATAAGCATGTAAGGATAATCAAACATCGTGTGCGCTGATGGCAGAGTAGGTGGAGGACTCCAGACAGTCAA                             >  SRR4302535.835217/1‑94 (MQ=60)
                                                                TTCTGAATTTTGGCCACGGAAAATAAGCATGTAAGGATAATCAAACATCGTGTGCGCTGATGGCAGAGTAGGTGGAGGACTCCAGACAGTCAAA                            >  SRR4302535.1047846/1‑94 (MQ=60)
                                                                TTCTGAATTTTGGCCACGGAAAATAAGCATGTAAGGATAATCAAACATCGTGTGCGCTGATGGCAGAGTAGGTGGAGGACTCCAGACAGTCAAA                            <  SRR4302535.2980380/94‑1 (MQ=60)
                                                                TTCTGAATTTTGGCCACGGAAAATAAGCATGTAAGGATAATCAAACATCGTGTGCGCTGATGGCAGAGTAGGTGGAGGACTCCAGACAGTCAAA                            >  SRR4302535.2591363/1‑94 (MQ=60)
                                                                 TCTGAATTTTGGCCACGGAAAATAAGCATGTAAGGATAATCAAACATCGTGTGCGCTGATGGCAGAGTAGGTGGAGGACTCCAGACAGTCAAAC                           <  SRR4302535.3648280/94‑1 (MQ=60)
                                                                 TCTGAATTTTGGCCACGGAAAATAAGCATGTAAGGATAATCAAACATCGTGTGCGCTGATGGCAGAGTAGGTGGAGGACTCCAGACAGTCAAAC                           <  SRR4302535.1948628/94‑1 (MQ=60)
                                                                  CTGAATTTTGGCCACGGAAAATAAGCATGTAAGGATAATCAAACATCGTGTGCGCTGATGGCAGAGTAGGTGGAGGACTCCAGACAGTCAAACG                          <  SRR4302535.1305475/94‑1 (MQ=60)
                                                                   TGAATTTTGGCCACGGAAAATAAGCATGTAAGGATAATCAAACATCGTGTGCGCTGATGGCAGAGTAGGTGGAGGACTCCAGACAGTCAAACGA                         >  SRR4302535.1160836/1‑94 (MQ=60)
                                                                   TGAATTTTGGCCACGGAAAATAAGCATGTAAGGATAATCAAACATCGTGTGCGCTGATGGCAGAGTAGGTGGAGGACTACAGACAGTCAAACGA                         >  SRR4302535.1309620/1‑94 (MQ=60)
                                                                    GAATTTTGGCCACGGAAAATAAGCATGTAAGGATAATCAAACATCGTGTGCGCTGATGGCAGAGTAGGTGGAGGACTCCAGACAGTCAAACGAT                        <  SRR4302535.2554029/94‑1 (MQ=60)
                                                                     AATTTTGGCCACGGAAAATAAGCATGTAAGGATAATCAAACATCGTGTGCGCTGATGGCAGAGTAGGTGGAGGACTCCAGACAGTCAAACGATA                       <  SRR4302535.2941429/94‑1 (MQ=60)
                                                                      ATTTTGGCCACGGAAAATAAGCATGTAAGGATAATCAAACATCGTGTGCGCTGATGGCAGAGTAGGTGGAGGACTCCAGACAGTCAAACGATAG                      <  SRR4302535.2056440/94‑1 (MQ=60)
                                                                      ATTTTGGCCACGGAAAATAAGCATGTAAGGATAATCAAACATCGTGTGCGCTGATGGCAGAGTAGGTGGAGGACTCCAGACAGTCAAACGATAG                      <  SRR4302535.1851895/94‑1 (MQ=60)
                                                                      ATTTTGGCCACGGAAAATAAGCATGTAAGGATAATCAAACATCGTGTGCGCTGATGGCAGAGTAGGTGGAGGACTCCAGACAGTCAAACGATAG                      <  SRR4302535.2286084/94‑1 (MQ=60)
                                                                      ATTTTGGCCACGGAAAATAAGCATGTAAGGATAATCAAACATCGTGTGCGCTGATGGCAGAGTAGGTGGAGGACTCCAGACAGTCAAACGATAG                      <  SRR4302535.3307623/94‑1 (MQ=60)
                                                                      ATTTTGGCCACGGAAAATAAGCATGTAAGGATAATCAAACATCGTGTGCGCTGATGGCAGAGTAGGTGGAGGACTCCAGACAGTCAAACGATAG                      <  SRR4302535.2936097/94‑1 (MQ=60)
                                                                          TGGCCACGGAAAATAAGCATGTAAGGATAATCAAACATCGTGTGCGCTGATGGCAGAGTAGGTGGAGGACTCCAGACAGTCAAACGATAGAAAA                  <  SRR4302535.253105/94‑1 (MQ=60)
                                                                          TGGCCACGGAAAATAAGCATGTAAGGATAATCAAACATCGTGTGCGCTGATGGCAGAGTAGGTGGAGGACTCCAGACAGTCAAACGATAGAAAA                  <  SRR4302535.187748/94‑1 (MQ=60)
                                                                           GGCCACGGAAAATAAGTATGTAAGGATAATCAAACATCGTGTGCGCTGATGGCAGAGTAGGTGGAGGACTCCAGACAGTCAAACGATAGAAAAA                 <  SRR4302535.4251317/94‑1 (MQ=60)
                                                                            GCCACGGAAAATAAGCATGTAAGGATAATCAAACATCGTGTGCGCTGATGGCAGAGTAGGTGGAGGACTCCAGACAGTCAAACGATAGAAAAAG                <  SRR4302535.4047086/94‑1 (MQ=60)
                                                                             CCACGGAAAATAAGCATGTAAGGATAATCAAACATCGTGTGCGCTGATGGCAGAGTAGGTGGAGGACTCCAGACAGTCAAACGATAGAAAAAGA               >  SRR4302535.136247/1‑94 (MQ=60)
                                                                              CACGGAAAATAAGCATGTAAGGATAATCAAACATCGTGTGCGCTGATGGCAGAGTAGGTGGAGGACTCCAGACAGTCAAACGATAGAAAAAGAT              >  SRR4302535.2030982/1‑94 (MQ=60)
                                                                              CACGGAAAATAAGCATGTAAGGATAATCAAACATCGTGTGCGCTGATGGCAGAGTAGGTGGAGGACTCCAGACAGTCAAACGATAGAAAAAGAT              >  SRR4302535.4349032/1‑94 (MQ=60)
                                                                              CACGGAAAATAAGCATGTAAGGATAATCAAACATCGTGTGCGCTGATGGCAGAGTAGGTGGAGGACTCCAGACAGTCAAACGATAGAAAAAGAT              >  SRR4302535.2218562/1‑94 (MQ=60)
                                                                               ACGGAAAATAAGCATGTAAGGATAATCAAACATCGTGTGCGCTGATGGCAGAGTAGGTGGAGGACTCCAGACAGTCAAACGATAGAAAAAGATA             <  SRR4302535.3244806/94‑1 (MQ=60)
                                                                               ACGGAAAATAAGCATGTAAGGATAATCAAACATCGTGTGCGCTGATGGCAGAGTAGGTGGAGGACTCCAGACAGTCAAACGATAGAAAAAGATA             <  SRR4302535.1039292/94‑1 (MQ=60)
                                                                                CGGAAAATAAGCATGTAAGGATAATCAAACATCGTGTGCGCTGATGGCAGAGTAGGTGGAGGACTCCAGACAGTCAAACGATAGAAAAAGATAG            <  SRR4302535.1670530/94‑1 (MQ=60)
                                                                                CGGAAAATAAGCATGTAAGGATAATCAAACATCGTGTGCGCTGATGGCAGAGTAGGTGGAGGACTCCAGACAGTCAAACGATAGAAAAAGATAG            <  SRR4302535.2982253/94‑1 (MQ=60)
                                                                                CGGAAAATAAGCATGTAAGGATAATCAAACATCGTGTGCGCTGATGGCAGAGTAGGTGGAGGACTCCAGACAGTCAAACGATAGAAAAAGATAG            >  SRR4302535.4196775/1‑94 (MQ=60)
                                                                                CGGAAAATAAGCATGTAAGGATAATCAAACATCGTGTGCGCTGATGGCAGAGTAGGTGGAGGACTCCAGACAGTCAAACGATAGAAAAAGATAG            <  SRR4302535.337546/94‑1 (MQ=60)
                                                                                 GGAAAATAAGCATGTAAGGATAATCAAACATCGTGTGCGCTGATGGCAGAGTAGGTGGAGGACTCCAGACAGTCAAACGATAGAAAAAGATAGC           <  SRR4302535.3680010/94‑1 (MQ=60)
                                                                                 GGAAAATAAGCATGTAAGGATAATCAAACATCGTGTGCGCTGATGGCAGAGTAGGTGGAGGACTCCAGACAGTCAAACGATAGAAAAAGATAGC           <  SRR4302535.1291233/94‑1 (MQ=60)
                                                                                   AAAATAAGCATGTAAGGATAATCAAACATCGTGTGCGCTGATGGCAGAGTAGGTGGAGGACTCCAGACAGTCAAACGATAGAAAAAGATAGCCT         <  SRR4302535.2442156/94‑1 (MQ=60)
                                                                                    AAATAAGCATGTAAGGATAATCAAACATCGTGTGCGCTGATGGCAGAGTAGGTGGAGGACTCCAGACAGTCAAACGATAGAAAAAGATAGCCTT        <  SRR4302535.135082/94‑1 (MQ=60)
                                                                                    AAATAAGCATGTAAGGATAATCAAACATCGTGTGCGCTGATGGCAGAGTAGGTGGAGGACTCCAGACAGTCAAACGATAGAAAAAGATAGCCTT        >  SRR4302535.2690572/1‑94 (MQ=60)
                                                                                    AAATAAGCATGTAAGGATAATCAAACATCGTGTGCGCTGATGGCAGAGTAGGTGGAGGACTCCAGACAGTCAAACGATAGAAAAAGATAGCCTT        <  SRR4302535.973209/94‑1 (MQ=60)
                                                                                     AATAAGCATGTAAGGATAATCAAACATCGTGTGCGCTGATGGCAGAGTAGGTGGAGGACTCCAGACAGTCAAACGATAGAAAAAGATAGCCTTT       <  SRR4302535.1967376/94‑1 (MQ=60)
                                                                                       TAAGCATGTAAGGATAATCAAACATCGTGTGCGCTGATGGCAGAGTAGGTGGAGGACTCCAGACAGTCAAACGATAGAAAAAGATAGCCTTTAT     <  SRR4302535.4014962/94‑1 (MQ=60)
                                                                                       TAAGCATGTAAGGATAATCAAACATCGTGTGCGCTGATGGCAGAGTAGGTGGAGGACTCCAGACAGTCAAACGATAGAAAAAGATAGCCTTTAT     <  SRR4302535.2076402/94‑1 (MQ=60)
                                                                                       TAAGCATGTAAGGATAATCAAACATCGTGTGCGCTGATGGCAGAGTAGGTGGAGGACTCCAGACAGTCAAACGATAGAAAAAGATAGCCTTTAT     <  SRR4302535.1463267/94‑1 (MQ=60)
                                                                                        AAGCATGTAAGGATAATCAAACATCGTGTGCGCTGATGGCAGAGTAGGTGGAGGACTCCAGACAGTCAAACGATAGAAAAAGATAGCCTTTATG    >  SRR4302535.4316222/1‑94 (MQ=60)
                                                                                        AAGCATGTAAGGATAATCAAACATCGTGTGCGCTGATGGCAGAGTAGGTGGAGGACTCCAGACAGTCAAACGATAGAAAAAGATAGCCTTTATG    <  SRR4302535.3485502/94‑1 (MQ=60)
                                                                                          GCATGTAAGGATAATCAAACATCGTGTGCGCTGATGGCAGAGTAGGTGGAGGACTCCAGACAGTCAAACGATAGAAAAAGATAGCCTTTATGGA  <  SRR4302535.3022078/94‑1 (MQ=60)
                                                                                           |                                                                                            
ATTCACTCAGACCAAATTGCTGGTGGGGTTTTTATTATAGAAATAGGCAAAAATGATGGGAGAATTCTGAATTTTGGCCACGGAAAATAAGTATGTAAGGATAATCAAACATCGTGTGCGCTGATGGCAGAGTAGGTGGAGGACTCCAGACAGTCAAACGATAGAAAAAGATAGCCTTTATGGA  >  NC_000913/3621324‑3621507

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑