Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 1873 183.1 9376148 98.2% 9207377 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,704,865 T→C K157R (AAG→AGG)  dppA ← dipeptide transport protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,704,8650TC99.5% 603.3 / ‑4.8 192K157R (AAG→AGG) dppAdipeptide transport protein
Reads supporting (aligned to +/- strand):  ref base T (0/1);  new base C (89/102);  total (89/103)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.43e-01

Only 100 of 213 total aligned reads displayed.

GACAGAATAGAGGCGAAGTCCATTGCCAGGTCAGCGAGGAACGGCGCTTCCGGGCGAGTCAGCACAAACTGAACGGTGTTGTCGTCCACCTTTTTCACTTCACTGATCAGCTCTGGCAAGCCCATGCCTTCGAAGTATTCGTAGCTGCCGCCAGAAACTTTATGGTACGGGTTTTGCGCG  >  NC_000913/3704775‑3704954
                                                                                          |                                                                                         
gACAGAATAGAGGCGAAGTCCATTGCCAGGTCAGCGAGGAACGGCGCTTCCGGGCGAGTCAGCACAAACTGAACGGTGTTGTCGTCCACCctt                                                                                         <  2:4250603/93‑1 (MQ=255)
 aCAGAATAGAGGCGAAGTCCATTGCCAGGTCAGCGAGGAACGGCGCTTCCGGGCGAGTCAGCACAAACTGAACGGTGTTGTCGTCCACCcttt                                                                                        <  2:3635028/93‑1 (MQ=255)
 aCAGAATAGAGGCGAAGTCCATTGCCAGGTCAGCGAGGAACGGCGCTTCCGGGCGAGTCAGCACAAACTGAACGGTGTTGTCGTCCACCcttt                                                                                        >  1:1335823/1‑93 (MQ=255)
 aCAGAATAGAGGCGAAGTCCATTGCCAGGTCAGCGAGGAACGGCGCTTCCGGGCGAGTCAGCACAAACTGAACGGTGTTGTCGTCCACCcttt                                                                                        >  1:1839723/1‑93 (MQ=255)
  cAGAATAGAGGCGAAGTCCATTGCCAGGTCAGCGAGGAACGGCGCTTCCGGGCGAGTCAGCACAAACTGAACGGTGTTGTCGTCCACCctttt                                                                                       <  2:1638470/93‑1 (MQ=255)
  cAGAATAGAGGCGAAGTCCATTGCCAGGTCAGCGAGGAACGGCGCTTCCGGGCGAGTCAGCACAAACTGAACGGTGTTGTCGTCCACCctttt                                                                                       >  1:3894142/1‑93 (MQ=255)
   aGAATAGAGGCGAAGTCCATTGCCAGGTCAGCGAGGAACGGCGCTTCCGGGCGAGTCAGCACAAACTGAACGGTGTTGTCGTCCACCCTTttc                                                                                      >  2:3293372/1‑93 (MQ=255)
    gAATAGAGGCGAAGTCCATTGCCAGGTCAGCGAGGAACGGCGCTTCCGGGCGAGTCAGCACAAACTGAACGGTGTTGTCGTCCACCCTTttca                                                                                     >  2:161576/1‑93 (MQ=255)
    gAATAGAGGCGAAGTCCATTGCCAGGTCAGCGAGGAACGGCGCTTCCGGGCGAGTCAGCACAAACTGAACGGTGTTGTCGTCCACCCTTttca                                                                                     >  1:1756676/1‑93 (MQ=255)
    gAATAGAGGCGAAGTCCATTGCCAGGTCAGCGAGGAACGGCGCTTCCGGGCGAGTCAGCACAAACTGAACGGTGTTGTCGTCCACCCTTttca                                                                                     >  1:640168/1‑93 (MQ=255)
    gAATAGAGGCGAAGTCCATTGCCAGGTCAGCGAGGAACGGCGCTTCCGGGCGAGTCAGCACAAACTGAACGGTGTTGTCGTCCACCCTTttca                                                                                     <  1:4484279/93‑1 (MQ=255)
      aTAGAGGCGAAGTCCATTGCCAGGTCAGCGAGGAACGGCGCTTCCGGGCGAGTCAGCACAAACTGAACGGTGTTGTCGTCCACCCTTttcact                                                                                   >  2:469167/1‑93 (MQ=255)
       tAGAGGCGAAGTCCATTGCCAGGTCAGCGAGGAACGGCGCTTCCGGGCGAGTCAGCACAAACTGAACGGTGTTGTCGTCCACCCTTttcactt                                                                                  >  1:2888272/1‑93 (MQ=255)
       tAGAGGCGAAGTCCATTGCCAGGTCAGCGAGGAACGGCGCTTCCGGGCGAGTCAGCACAAACTGAACGGTGTTGTCGTCCACCCTTttcactt                                                                                  >  2:3736331/1‑93 (MQ=255)
       tAGAGGCGAAGTCCATTGCCAGGGCAGCGAGGAACGGCGCTTCCGGGCGAGTCAGCACAAACTGAACGGTGTTGTCGTCCACCCTTttcactt                                                                                  >  1:3158200/1‑93 (MQ=255)
         gagGCGAAGTCCATTGCCAGGTCAGCGAGGAACGGCGCTTCCGGGCGAGTCAGCACAAACTGAACGGTGTTGTCGTCCACCCTTttcacttca                                                                                >  2:714592/1‑93 (MQ=255)
          agGCGAAGTCCATTGCCAGGTCAGCGAGGAACGGCGCTTCCGGGCGAGTCAGCACAAACTGAACGGTGTTGTCGTCCACCCTTttcacttcac                                                                               >  2:186077/1‑93 (MQ=255)
           ggCGAAGTCCATTGCCAGGTCAGCGAGGAACGGCGCTTCCGGGCGAGTCAGCACAAACTGAACGGTGTTGTCGTCCACCCTTTtcacttcact                                                                              >  1:3752714/1‑93 (MQ=255)
            gCGAAGTCCATTGCCAGGTCAGCGAGGAACGGCGCTTCCGGGCGAGTCAGCACAAACTGAACGGTGTTGTCGTCCACCCTTTTCACTTCACTg                                                                             <  2:3103637/93‑1 (MQ=255)
            gCGAAGTCCATTGCCAGGTCAGCGAGGAACGGCGCTTCCGGGCGAGTCAGCACAAACTGAACGGTGTTGTCGTCCACCCTTTTCACTTCACTg                                                                             <  1:1135656/93‑1 (MQ=255)
             cGAAGTCCATTGCCAGGTCAGCGAGGAACGGCGCTTCCGGGCGAGTCAGCACAAACTGAACGGTGTTGTCGTCCACCCTTTTCCCTTCACTGa                                                                            <  2:3908528/93‑1 (MQ=255)
               aaGTCCATTGCCAGGTCAGCGAGGAACGGCGCTTCCGGGCGAGTCAGCACAAACTGAACGGTGTTGTCGTCCACCCTTTTCACTTCACTGATc                                                                          >  2:1107092/1‑93 (MQ=255)
                aGTCCATTGCCAGGTCAGCGAGGAACGGCGCTTCCGGGCGAGTCAGCACAAACTGAACGGTGTTGTCGTCCACCCTTTTCACTTCACTGATCa                                                                         <  2:1776904/93‑1 (MQ=255)
                 gTCCATTGCCAGGTCAGCGAGGAACGGCGCTTCCGGGCGAGTCAGCACAAACTGAACGGTGTTGTCGTCCACCCTTTTCACTTCACTGATCAg                                                                        >  1:2440314/1‑93 (MQ=255)
                 gTCCATTGCCAGGTCAGCGAGGAACGGCGCTTCCGGGCGAGTCAGCACAAACTGAACGGTGTTGTCGTCCACCCTTTTCACTTCACTGATCAg                                                                        >  1:1924187/1‑93 (MQ=255)
                 gTCCATTGCCAGGTCAGCGAGGAACGGCGCTTCCGGGCGAGTCAGCACAAACTGAACGGTGTTGTCGTCCACCCTTTTCACTTCACTGATCAg                                                                        >  1:1716043/1‑93 (MQ=255)
                  tCCATTGCCAGGTCAGCGAGGAACGGCGCTTCCGGGCGAGTCAGCACAAACTGAACGGTGTTGTCGTCCACCCTTTTCACTTCACTGATCAGc                                                                       >  1:531505/1‑93 (MQ=255)
                  tCCATTGCCAGGTCAGCGAGGAACGGCGCTTCCGGGCGAGTCAGCACAAACTGAACGGTGTTGTCGTCCACCCTTTTCACTTCACTGATCAGc                                                                       <  1:2002629/93‑1 (MQ=255)
                     aTTGCCAGGTCAGCGAGGAACGGCGCTTCCGGGCGAGTCAGCACAAACTGAACGGTGTTGTCGTCCACCCTTTTCACTTCACTGATCAGctct                                                                    >  1:3524529/1‑93 (MQ=255)
                     aTTGCCAGGTCAGCGAGGAACGGCGCTTCCGGGCGAGTCAGCACAAACTGAACGGTGTTGTCGTCCACCCTTTTCACTTCACTGATCAGctct                                                                    >  1:1439331/1‑93 (MQ=255)
                     aTTGCCAGGTCAGCGAGGAACGGCGCTTCCGGGCGAGTCAGCACAAACTGAACGGTGTTGTCGTCCACCCTTTTCACTTCACTGATCAGctct                                                                    >  1:4197010/1‑93 (MQ=255)
                     aTTGCCAGGTCAGCGAGGAACGGCGCTTCCGGGCGAGTCAGCACAAACTGAACGGTGTTGTCGTCCACCCTTTTCACTTCACTGATCAGctct                                                                    <  2:4352762/93‑1 (MQ=255)
                     aTTGCCAGGTCAGCGAGGAACGGCGCTTCCGGGCGAGTCAGCACAAACTGAACGGTGTTGTCGTCCACCCTTTTCACTTCACTGATCAGctct                                                                    >  2:3028592/1‑93 (MQ=255)
                      ttGCCAGGTCAGCGAGGAACGGCGCTTCCGGGCGAGTCAGCACAAACTGAACGGTGTTGTCGTCCACCCTTTTCACTTCACTGATCAGCTCTg                                                                   >  2:699933/1‑93 (MQ=255)
                      ttGCCAGGTCAGCGAGGAACGGCGCTTCCGGGCGAGTCAGCACAAACTGAACGGTGTTGTCGTCCACCCTTTTCACTTCACTGATCAGCTCTg                                                                   >  2:3596361/1‑93 (MQ=255)
                         ccAGGTCAGCGAGGAACGGCGCTTCCGGGCGAGTCAGCACAAACTGAACGGTGTTGTCGTCCACCCTTTTCACTTCACTGATCAGCTCTGGCa                                                                >  1:3798031/1‑93 (MQ=255)
                         ccAGGTCAGCGAGGAACGGCGCTTCCAGGCGAGTCAGCACAAACTGAACGGTGTTGTCGTCCACCCTTTTCACTTCACTGATCAGCTCTGGCa                                                                <  1:1905398/93‑1 (MQ=255)
                          cAGGTCAGCGAGGAACGGCGCTTCCGGGCGAGTCAGCACAAACTGAACGGTGTTGTCGTCCACCCTTTTCACTTCACTGATCAGCTCTGGCaa                                                               >  1:2335717/1‑93 (MQ=255)
                           aGGTCAGCGAGGAACGGCGCTTCCGGGCGAGTCAGCACAAACTGAACGGTGTTGTCGTCCACCCTTTTCACTTCACTGATCAGCTTTTGCaac                                                              >  2:1590935/1‑92 (MQ=255)
                            ggTCAGCGAGGAACGGCGCTTCCGGGCGAGTCAGCACAAACTGAACGGTGTTGTCGTCCACCCTTTTCACTTCACTGATCAGCTCTGGCAAGc                                                             >  1:2802047/1‑93 (MQ=255)
                            ggTCAGCGAGGAACGGCGCTTCCGGGCGAGTCAGCACAAACTGAACGGTGTTGTCGTCCACCCTTTTCACTTCACTGATCAGCTCTGGCAAGc                                                             >  1:3434984/1‑93 (MQ=255)
                               cAGCGAGGAACGGCGCTTCCGGGCGAGTCAGCACAAACTGAACGGTGTTGTCGTCCACCTTTTTCACTTCACTGATCAGCTCTGGCAAGCCCa                                                          <  1:1086825/93‑1 (MQ=255)
                               cAGCGAGGAACGGCGCTTCCGGGCGAGTCAGCACAAACTGAACGGTGTTGTCGTCCACCCTTTTCACTTCCCTGATCAGCTCTGGCAAGCCCa                                                          <  1:2726834/93‑1 (MQ=255)
                                aGCGAGGAACGGCGCTTCCGGGCGAGTCAGCACAAACTGAACGGTGTTGTCGTCCACCCTTTTCACTTCACTGATCAGCTCTGGCAAGCCCAt                                                         <  2:50596/93‑1 (MQ=255)
                                 gCGAGGAACGGGGCTTCCGGGCGAGTCAGCACAAACTGAACGGTGTTGTCGTCCACCCTTTTCACTTCACTGATCAGCTCTGGCAAGCCCATg                                                        >  1:2742968/1‑93 (MQ=255)
                                 gCGAGGAACGGCGCTTCCGGGCGAGTCAGCACAAACTGAACGGTGTTGTCGTCCACCCTTTTCACTTCACTGATCAGCTCTGGCAAGCCCATg                                                        >  1:474964/1‑93 (MQ=255)
                                  cGAGGAACGGCGCTTCCGGGCGAGTCAGCACAAACTGAACGGTGTTGTCGTCCACCCTTTTCACTTCACTGATCAGCTCTGGCAAGCCCATGc                                                       <  2:340895/93‑1 (MQ=255)
                                   gAGGAACGGCGCTTCCGGGCGAGTCAGCACAAACTGAACGGTGTTGTCGTCCACCCTTTTCACTTCACTGATCAGCTCTGGCAAGCCCATGcc                                                      <  1:2631708/93‑1 (MQ=255)
                                   gAGGAACGGCGCTTCCGGGCGAGTCAGCACAAACTGAACGGTGTTGTCGTCCACCCTTTTCACTTCACTGATCAGCTCTGGCAAGCCCATGcc                                                      <  2:1670637/93‑1 (MQ=255)
                                     ggAACGGCGCTTCCGGGCGAGTCAGCACAAACTGAACGGTGTTGTCGTCCACCCTTTTCACTTCACTGATCAGCTCTGGCAAGCCCATGCCtt                                                    >  2:2610210/1‑93 (MQ=255)
                                        aCGGCGCTTCCGGGCGAGTCAGCACAAACTGAACGGTGTTGTCGTCCACCCTTTTCACTTCACTGATCAGCTCTGGCAAGCCCATGCCTTCGa                                                 >  1:3280679/1‑93 (MQ=255)
                                         cGGCGCTTCCGGGCGAGTCAGCACAAACTGAACGGTGTTGTCGTCCACCCTTTTCACTTCACTGATCAGCTCTGGCAAGCCCATGCCTTCGaa                                                <  2:2661578/93‑1 (MQ=255)
                                          ggCGCTTCCGGGCGAGTCAGCACAAACTGAACGGTGTTGTCGTCCACCCTTTTCACTTCACTGATCAGCTCTGGCAAGCCCATGCCTTCGAAg                                               <  2:4513683/93‑1 (MQ=255)
                                            cgcTTCCGGGCGAGTCAGCACAAACTGAACAGTGTTGTCGTCCACCCTTTTCACTTCACTGATCAGCTCTGGCAAGCCCATGCCTTCGAAGTa                                             <  2:4599417/93‑1 (MQ=255)
                                             gcTTCCGGGCGAGTCAGCACAATCTGAACGGTGTTGTCGTCCACCCTTTTCACTTCCCTGATCAGCTCTGGCAAGCCCATGCCTTCGAAGTAt                                            <  2:2940975/93‑1 (MQ=255)
                                             gcTTCCGGGCGAGTCAGCACAAACTGAACGGTGTTGTCGTCCACCCTTTTCACTTCACTGATCAGCTCTGGCAAGCCCATGCCTTCGAAGTAt                                            <  2:58265/93‑1 (MQ=255)
                                             gcTTCCGGGCGAGTCAGCACAAACTGAACGGTGTTGTCGTCCACCCTTTTCACTTCACTGATCAGCTCTGGCAAGCCCATGCCTTCGAAGTAt                                            <  1:714010/93‑1 (MQ=255)
                                              cTTCCGGGCGAGTCAGCACAAACTGAACGGTGTTGTCGTCCACCCTTTTCACTTCACTGATCAGCTCTGGCAAGCCCATGCCTTCGAAGTAtt                                           <  2:1174134/93‑1 (MQ=255)
                                               ttCCGGGCGAGTCAGCACAAACTGAACGGTGTTGTCGTCCACCCTTTTCACTTCACTGATCAGCTCTGGCAAGCCCATGCCTTCGAAGTATTc                                          <  2:2186179/93‑1 (MQ=255)
                                                 ccGGGCGAGTCAGCACAAACTGAACGGTGTTGTCGTCCACCCTTTTCACTTCACTGATCAGCTCTGGCAAGCCCATGCCTTCGAAGTATTCGt                                        >  1:128360/1‑93 (MQ=255)
                                                   gggCGAGTCAGCACAAACTGAACGGTGTTGTCGTCCACCCTTTTCACTTCACTGATCAGCTCTGGCAAGCCCATGCCTTCGAAGTATTCGTAg                                      >  1:563211/1‑93 (MQ=255)
                                                   gggCGAGTCAGCACAAACTGAACGGTGTTGTCGTCCACCCTTTTCACTTCACTGATCAGCTCTGGCAAGCCCATGCCTTCGAAGTATTCGTAg                                      <  1:2983554/93‑1 (MQ=255)
                                                    ggCGAGTCAGCACAAACTGAACGGTGTTGTCGTCCACCCTTTTCACTTCACTGATCAGCTCTGGCAAGCCCATGCCTTCGAAGTATTCGTAGc                                     <  2:2042052/93‑1 (MQ=255)
                                                    ggCGAGTCAGCACAAACTGAACGGTGTTGTCGTCCACCCTTTTCACTTCACTGATCAGCTCTGGCAAGCCCATGCCTTCGAAGTATTCGTAGc                                     <  2:4306627/93‑1 (MQ=255)
                                                    ggCAAGTCAGCACAAACTGAACGGTGTTGTCGTCCACCCTTTTCACTTCACTGATCAGCTCTGCCAAGCCCATGCCTTCGAAGTATTCGTAGc                                     <  2:2111213/93‑1 (MQ=255)
                                                       gAGTCAGCACAAACTGAACGGTGTTGTCGTCCACCCTTTTCACTTCACTGATCAGCTCTGGCAAGCCCATGCCTTCGAAGTATTCGTAGCTgc                                  >  2:1966875/1‑93 (MQ=255)
                                                        aGTCAGCACAAACTGAACGGTGTTGTCGTCCACCCTTTTCACTTCACTGATCAGCTCTGGCAAGCCCATGCCTTCGCAGTATTCGTAGCTgcc                                 >  1:1724208/1‑93 (MQ=255)
                                                        aGTCAGCACAAACTGAACGGTGTTGTCGTCCACCCTTTTCACTTCACTGATCAGCTCTGGAAAGCCCATGCCTTCGAAGTATTCGTAGCTgcc                                 >  1:1028957/1‑93 (MQ=255)
                                                         gTCAGCACAAACTGAACGGTGTTGTCGTCCACCCTTTTCACTTCACTGATCAGCTCTGGCAAGCCCATGCCTTCGAAGTATTCGTAGCTgccg                                >  1:2651680/1‑93 (MQ=255)
                                                          tCAGCACAAACTGAACGGTGTTGTCGTCCACCCTTTTCACTTCACTGATCAGCTCTGGCAAGCCCATGCCTTCGAAGTATTCGTAGCTgccgc                               >  1:578945/1‑93 (MQ=255)
                                                           cAGCACAAACTGAACGGTGTTGTCGTCCACCCTTTTCACTTCACTGATCAGCTCTGGCAAGACCATGCCTTAGAAGTATTCGTAGCTgccgcc                              <  2:2335939/93‑1 (MQ=255)
                                                             gCACAAACTGAACGGTGTTGTCGTCCACCCTTTTCACTTCACTGATCAGCTCTGGCAAGCCCATGCCTTCGAAGTATTCGTAGCTGCCGCCAg                            >  1:2070093/1‑93 (MQ=255)
                                                             acacaAACTGAACGGTGTTGTCGTCCACCCTTTTCACTTCACTGATCAGCTCTGGCAAGCCCATGCCTTCGAAGTATTCGTAGCTGCCGCCAg                            <  2:929347/92‑1 (MQ=255)
                                                               acaAACTGAACGGTGTTGTCGTCCACCCTTTTCACTTCACTGATCAGCTCTGGCAAGCCCATGCCTTCGAAGTATTCGTAGCTGCCGCCAGaa                          >  2:1357466/1‑93 (MQ=255)
                                                               acaAACTGAACGGTGTTGTCGTCCACCCTTTTCACTTCACTGATCAGCTCTGGCAAGCCCATGCCTTCGAAGTATTCGTAGCTGCCGCCAGaa                          <  2:1036025/93‑1 (MQ=255)
                                                               acaAACTGAACGGTGTTGTCGTCCACCCTTTTCACTTCACTGATCAGCTCTGGCAAGCCCATGCCTTCGAAGTATTCGTAGCTGCCGCCAGaa                          >  1:1622815/1‑93 (MQ=255)
                                                                  aaCTGAACGGTGTTGTCGTCCACCCTTTTCACTTCACTGATCAGCTCTGGCAAGCCCATGCCTTCGAAGTATTCGTAGCTGCCGCCAGAAACt                       >  2:639877/1‑93 (MQ=255)
                                                                   aCTGAACGGTGTTGTCGTCCACCCTTTTCACTTCACTGATCAGCTCTGGCAAGCCCATGCCTTCGAAGTATTCGTAGCTGCCGCCAGAAACtt                      >  2:3977624/1‑93 (MQ=255)
                                                                     tGAACGGTGTTGTCGTCCACCCTTTTCACTTCACTGATCAGCTCTGGCAAGCCCATGCCTTCGAAGTATTCGTAGCTGCCGCCAGAAACTTTa                    <  2:3466877/93‑1 (MQ=255)
                                                                     tGAACGGTGTTGTCGTCCACCCTTTTCACTTCACTGATCAGCTCTGGCAAGCCCATGCCTTCGAAGTATTCGTAGCTGCCGCCAGAAACTTTa                    >  2:727472/1‑93 (MQ=255)
                                                                      gAACGGTGTTGTCGTCCACCCTTTTCACTTCACTGATCAGCTCTGGCAAGCCCATGCCTTCGAAGTATTCGTAGCTGCCGCCAGAAACTTTAt                   <  1:3059964/93‑1 (MQ=255)
                                                                       aaCGGTGTTGTCGTCCACCCTTTTCACTTCACTGATCAGCTCTGGCAAGCCCATGCCTTCGAAGTATTCGTAGCTGCCGCCAGAAACTTTATg                  <  1:394224/93‑1 (MQ=255)
                                                                       aaCGGTGTTGTCGTCCACCCTTTTCACTTCACTGATCAGCTCTGGCAAGCCCATGCCTTCGAAGTATTCGTAGCTGCCGCCAGAAACTTTATg                  <  2:4511437/93‑1 (MQ=255)
                                                                        aCGGTGTTGTCGTCCACCCTTTTCACTTCACTGATCAGCTCTGGCAAGCCCATGCCTTCGAAGTATTCGTAGCTGCCGCCAGAAACTTTATgg                 <  1:3228236/93‑1 (MQ=255)
                                                                        aCGGTGTTGTCGTCCACCCTTTTCACTTCACTGATCAGCTCTGGCAAGCCCATGCCTTCGAAGTATTCGTAGCTGCCGCCAGAAACTTTATgg                 >  2:2830902/1‑93 (MQ=255)
                                                                          ggTGTTGTCGTCCACCCTTTTCACTTCACTGATCAGCTCTGGCAAGCCCATGCCTTCGAAGTATTCGTAGCTGCCGCCAGAAACTTTATGGTa               <  1:780511/93‑1 (MQ=255)
                                                                          ggTGTTGTCGTCCACCCTTTTCACTTCACTGATCAGCTCTGGCAAGCCCATGCCTTCGAAGTATTCGTAGCTGCCGCCAGAAACTTTATGGTa               <  1:406886/93‑1 (MQ=255)
                                                                           gtgtTGTCGTCCACCCTTTTCGCTTCACTGATCAGCTCTGGCAAGCCCATGCCTTCGAAGTATTCGTAGCTGCCGCCAGAAACTTTATGGTAc              <  1:1545989/93‑1 (MQ=255)
                                                                           gtgtTGTCGTCCACCCTTTTCACTTCACTGATCAGCTCTGGCAAGCCCATGCCTTCGAAGTATTCGTAGCTGCCGCCAGAAACTTTATGGTAc              >  2:3535282/1‑93 (MQ=255)
                                                                           gtgtTGTCGTCCACCCTTTTCACTTCACTGATCAGCTCTGGCAAGCCCATGCCTTCGAAGTATTCGTAGCTG‑CGCCAGAAACTTTATGGTACg             >  1:2688338/1‑93 (MQ=255)
                                                                              ttgtCGTCCACCCTTTTCACTTCACTGATCAGCTCTGGCAAGCCCATGCCTTCGAAGTATTCGTAGCTGCCGCCAGAAACTTTATGGTACggg           >  2:2523726/1‑93 (MQ=255)
                                                                              ttgtCGTCCACCCTTTTCACTTCACTGATCAGCTCTGGCAAGCCCATGCCTTCGAAGTATTCGTAGCTGCCGCCAGAAACTTTATGGTACggg           <  1:4431228/93‑1 (MQ=255)
                                                                               tgtCGTCCACCCTTTTCACTTCACTGATCAGCTCTGGCAAGCCCATGCCTCCGAAGTATTCGTAGCTGCCGCCAGAAACTTTATGGTACGGGt          <  1:2509869/93‑1 (MQ=255)
                                                                                gtcgtcCACCCTTTTCACTTCACTGATCAGCTCTGGCAAGCCCATGCCTTCGAAGTATTCGTAGCTGCCGCCAGAAACTTTATGGTACgggct         >  1:665931/1‑91 (MQ=255)
                                                                                gtcgtcCACCCTTTTCACTTCACTGATCAGCTCTGGCAAGCCCATGCCTTCGAAGTATTCGTAGCTGCCGCCAGAAACTTTATGGTACGGGtt         <  2:37719/93‑1 (MQ=255)
                                                                                 tcgtcCACCCTTTTCACTTCACTGATCAGCTCTGGCAAGCCCATGCCTTCGAAGTATTCGTAGCTGCCGCCAGAAACTTTATGGTACGGGttt        >  2:531354/1‑93 (MQ=255)
                                                                                    tcCACCCTTTTCACTTCACTGATCAGCTCTGGCAAGCCCATGCCTTCGAAGTATTCGTAGCTGCCGCCAGAAACTTTATGGTACGGGTTTTgc     >  2:3858259/1‑93 (MQ=255)
                                                                                    tcCACCCTTTTCACTTCACTGATCAGCTCTGGCAAGCCCATGCCTTCGAAGTATTCGTAGCTGCCGCCAGAAACTTTATGGTACGGGTTTTgc     <  2:2090981/93‑1 (MQ=255)
                                                                                      cACCCTTTTCACTTCACTGATCAGCTCTGGCAAGCCCATGCCTTCGAAGTATTCGTAGCTGCCGCCAGAAACTTTATGGTACGGGTTTTgcgc   <  1:880091/93‑1 (MQ=255)
                                                                                      cACCCTTTTCACTTCACTGATCAGCTCTGGCAAGCCCATGCCTTCGAAGTATTCGTAGCTGCCGCCAGAAACTTTATGGTACGGGTTTTgcgc   <  2:2490419/93‑1 (MQ=255)
                                                                                          |                                                                                         
GACAGAATAGAGGCGAAGTCCATTGCCAGGTCAGCGAGGAACGGCGCTTCCGGGCGAGTCAGCACAAACTGAACGGTGTTGTCGTCCACCTTTTTCACTTCACTGATCAGCTCTGGCAAGCCCATGCCTTCGAAGTATTCGTAGCTGCCGCCAGAAACTTTATGGTACGGGTTTTGCGCG  >  NC_000913/3704775‑3704954

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
TGACAGAATAGAGGCGAAGTCCATTGCCAGGTCAGCGAGGAACGGCGCTTCCGGGCGAGTCAGCACAAACTGAACGGTGTTGTCGTCCACCTTTTTCACTTCACTGATCAGCTCTGGCAAGCCCATGCCTTCGAAGTATTCGTAGCTGCCGCCAGAAACTTTATGGTACGGGTTTTGCGCGTTTT  >  NC_000913/3704774‑3704958
                                                                                           |                                                                                             
TGACAGAATAGAGGCGAAGTCCATTGCCAGGTCAGCGAGGAACGGCGCTTCCGGGCGAGTCAGCACAAACTGAACGGTGTTGTCGTCCACCCTT                                                                                             <  SRR4302535.3049530/94‑1 (MQ=60)
TGACAGAATAGAGGCGAAGTCCATTGCCAGGTCAGCGAGGAACGGCGCTTCCGGGCGAGTCAGCACAAACTGAACGGTGTTGTCGTCCACCCTT                                                                                             <  SRR4302535.2166404/94‑1 (MQ=60)
TGACAGAATAGAGGCGAAGTCCATTGCCAGGTCAGCGAGGAACGGCGCTTCCGGGCGAGTCAGCACAAACTGAACGGTGTTGTCGTCCACCCTT                                                                                             <  SRR4302535.1436365/94‑1 (MQ=60)
 GACAGAATAGAGGCGAAGTCCATTGCCAGGTCAGCGAGGAACGGCGCTTCCGGGCGAGTCAGCACAAACTGAACGGTGTTGTCGTCCACCCTTT                                                                                            >  SRR4302535.1852994/1‑94 (MQ=60)
 GACAGAATAGAGGCGAAGTCCATTGCCAGGTCAGCGAGGAACGGCGCTTCCGGGCGAGTCAGCACAAACTGAACGGTGTTGTCGTCCACCCTTT                                                                                            >  SRR4302535.1345352/1‑94 (MQ=60)
  ACAGAATAGAGGCGAAGTCCATTGCCAGGTCAGCGAGGAACGGCGCTTCCGGGCGAGTCAGCACAAACTGAACGGTGTTGTCGTCCACCCTTTT                                                                                           <  SRR4302535.1831537/94‑1 (MQ=60)
  ACAGAATAGAGGCGAAGTCCATTGCCAGGTCAGCGAGGAACGGCGCTTCCGGGCGAGTCAGCACAAACTGAACGGTGTTGTCGTCCACCCTTTT                                                                                           >  SRR4302535.3920834/1‑94 (MQ=60)
  ACAGAATAGAGGCGAAGTCCATTGCCAGGTCAGCGAGGAACGGCGCTTCCGGGCGAGTCAGCACAAACTGAACGGTGTTGTCGTCCACCCTTTT                                                                                           <  SRR4302535.3897232/94‑1 (MQ=60)
   CAGAATAGAGGCGAAGTCCATTGCCAGGTCAGCGAGGAACGGCGCTTCCGGGCGAGTCAGCACAAACTGAACGGTGTTGTCGTCCACCCTTTTC                                                                                          >  SRR4302535.636674/1‑94 (MQ=60)
   CAGAATAGAGGCGAAGTCCATTGCCAGGCCAGCGAGGAACGGCGCTTCCGGGCGAGCAAGCACAAACTGAACGGTGTTGTCGCCCACCCTTTTC                                                                                          >  SRR4302535.1720221/1‑94 (MQ=60)
    AGAATAGAGGCGAAGTCCATTGCCAGGTCAGCGAGGAACGGCGCTTCCGGGCGAGTCAGCACAAACTGAACGGTGTTGTCGTCCACCCTTTTCA                                                                                         >  SRR4302535.1769375/1‑94 (MQ=60)
    AGAATAGAGGCGAAGTCCATTGCCAGGTCAGCGAGGAACGGCGCTTCCGGGCGAGTCAGCACAAACTGAACGGTGTTGTCGTCCACCCTTTTCA                                                                                         >  SRR4302535.644641/1‑94 (MQ=60)
    AGAATAGAGGCGAAGTCCATTGCCAGGTCAGCGAGGAACGGCGCTTCCGGGCGAGTCAGCACAAACTGAACGGTGTTGTCGTCCACCCTTTTCA                                                                                         >  SRR4302535.2659169/1‑94 (MQ=60)
    AGAATAGAGGCGAAGTCCATTGCCAGGTCAGCGAGGAACGGCGCTTCCGGGCGAGTCAGCACAAACTGAACGGTGTTGTCGTCCACCCTTTTCA                                                                                         <  SRR4302535.3537769/94‑1 (MQ=60)
     GAATAGAGGCGAAGTCCATTGCCAGGTCAGCGAGGAACGGCGCTTCCGGGCGAGTCAGCACAAACTGAACGGTGTTGTCGTCCACCCTTTTCAC                                                                                        <  SRR4302535.4515064/94‑1 (MQ=60)
      AATAGAGGCGAAGTCCATTGCCAGGTCAGCGAGGAACGGCGCTTCCGGGCGAGTCAGCACAAACTGAACGGTGTTGTCGTCCACCCTTTTCACT                                                                                       >  SRR4302535.395815/1‑94 (MQ=60)
       ATAGAGGCGAAGTCCATTGCCAGGTCAGCGAGGAACGGCGCTTCCGGGCGAGTCAGCACAAACTGAACGGTGTTGTCGTCCACCCTTTTCACTT                                                                                      >  SRR4302535.193681/1‑94 (MQ=60)
       ATAGAGGCGAAGTCCATTGCCAGGTCAGCGAGGAACGGCGCTTCCGGGCGAGTCAGCACAAACTGAACGGTGTTGTCGTCCACCCTTTTCACTT                                                                                      >  SRR4302535.2908707/1‑94 (MQ=60)
       ATAGAGGCGAAGTCCATTGCCAGGGCAGCGAGGAACGGCGCTTCCGGGCGAGTCAGCACAAACTGAACGGTGTTGTCGTCCACCCTTTTCACTT                                                                                      >  SRR4302535.3180120/1‑94 (MQ=60)
           AGGCGAAGTCCATTGCCAGGTCAGCGAGGAACGGCGCTTCCGGGCGAGTCAGCACAAACTGAACGGTGTTGTCGTCCACCCTTTTCACTTCACT                                                                                  >  SRR4302535.3778684/1‑94 (MQ=60)
            GGCGAAGTCCATTGCCAGGTCAGCGAGGAACGGCGCTTCCGGGCGAGTCAGCACAAACTGAACGGTGTTGTCGTCCACCCTTTTCACTTCACTG                                                                                 >  SRR4302535.82197/1‑94 (MQ=60)
            GGCGAAGTCCATTGCCAGGTCAGCGAGGAACGGCGCTTCCGGGCGAGTCAGCACAAACTGAACGGTGTTGTCGTCCACCCTTTTCACTTCACTG                                                                                 <  SRR4302535.1176369/94‑1 (MQ=60)
             GCGAAGTCCATTGCCAGGTCAGCGAGGAACGGCGCTTCCGGGCGAGTCAGCACAAACTGAACGGTGTTGTCGTCCACCCTTTTCACTTCACTGA                                                                                <  SRR4302535.1143755/94‑1 (MQ=60)
              CGAAGTCCATTGCCAGGTCAGCGAGGAACGGCGCTTCCGGGCGAGTCAGCACAAACTGAACGGTGTTGTCGTCCACCCTTTTCACTTCACTGAT                                                                               <  SRR4302535.6653/94‑1 (MQ=60)
               GAAGTCCATTGCCAGGTCAGCGAGGAACGGCGCTTCCGGGCGAGTCAGCACAAACTGAACGGTGTTGTCGTCCACCCTTTTCACTTCACTGATC                                                                              <  SRR4302535.1732695/94‑1 (MQ=60)
                 AGTCCATTGCCAGGTCAGCGAGGAACGGCGCTTCCGGGCGAGTCAGCACAAACTGAACGGTGTTGTCGTCCACCCTTTTCACTTCACTGATCAG                                                                            >  SRR4302535.1091831/1‑94 (MQ=60)
                 AGTCCATTGCCAGGTCAGCGAGGAACGGCGCTTCCGGGCGAGTCAGCACAAACTGAACGGTGTTGTCGTCCACCCTTTTCACTTCACTGATCAG                                                                            >  SRR4302535.1728458/1‑94 (MQ=60)
                 AGTCCATTGCCAGGTCAGCGAGGAACGGCGCTTCCGGGCGAGTCAGCACAAACTGAACGGTGTTGTCGTCCACCCTTTTCACTTCACTGATCAG                                                                            >  SRR4302535.2457894/1‑94 (MQ=60)
                 AGTCCATTGCCAGGTCAGCGAGGAACGGCGCTTCCGGGCGAGTCAGCACAAACTGAACGGTGTTGTCGTCCACCCTTTTCACTTCACTGATCAG                                                                            >  SRR4302535.1938056/1‑94 (MQ=60)
                  GTCCATTGCCAGGTCAGCGAGGAACGGCGCTTCCGGGCGAGTCAGCACAAACTGAACGGTGTTGTCGTCCACCCTTTTCACTTCACTGATCAGC                                                                           >  SRR4302535.535223/1‑94 (MQ=60)
                   TCCATTGCCAGGTCAGCGAGGAACGGCGCTTCCGGGCGAGTCAGCACAAACTGAACGGTGTTGTCGTCCACCCTTTTCACTTCACTGATCAGCT                                                                          <  SRR4302535.1750641/94‑1 (MQ=60)
                   TCCATTGCCAGGTCAGCGAGGAACGGCGCTTCCGGGCGAGTCAGCACAAACTGAACGGTGTTGTCGTCCACCCTTTTCACTTCACTGATCAGCT                                                                          <  SRR4302535.2017060/94‑1 (MQ=60)
                     CATTGCCAGGTCAGCGAGGAACGGCGCTTCCGGGCGAGTCAGCACAAACTGAACGGTGTTGTCGTCCACCCTTTTCACTTCACTGATCAGCTCT                                                                        >  SRR4302535.1449619/1‑94 (MQ=60)
                     CATTGCCAGGTCAGCGAGGAACGGCGCTTCCGGGCGAGTCAGCACAAACTGAACGGTGTTGTCGTCCACCCTTTTCACTTCACTGATCAGCTCT                                                                        >  SRR4302535.4225729/1‑94 (MQ=60)
                     CATTGCCAGGTCAGCGAGGAACGGCGCTTCCGGGCGAGTCAGCACAAACTGAACGGTGTTGTCGTCCACCCTTTTCACTTCACTGATCAGCTCT                                                                        >  SRR4302535.3549007/1‑94 (MQ=60)
                      ATTGCCAGGTCAGCGAGGAACGGCGCTTCCGGGCGAGTCAGCACAAACTGAACGGTGTTGTCGTCCACCCTTTTCACTTCACTGATCAGCTCTG                                                                       >  SRR4302535.3297191/1‑94 (MQ=60)
                      ATTGCCAGGTCAGCGAGGAACGGCGCTTCCGGGCGAGTCAGCACAAACTGAACGGTGTTGTCGTCCACCCTTTTCACTTCACTGATCAGCTCTG                                                                       <  SRR4302535.2539178/94‑1 (MQ=60)
                      ATTGCCAGGTCAGCGAAGAACGGCGCTTCCGGGCGAGTCAGCACAAACTGAACGGTGTTGTCGTCCACCCTTTTCACTTCACTGATCAGCTCTG                                                                       >  SRR4302535.1102348/1‑94 (MQ=60)
                         GCCAGGTCAGCGAGGAACGGCGCTTCCGGGCGAGTCAGCACAAACTGAACGGTGTTGTCGTCCACCCTTTTCACTTCACTGATCAGCTCTGGCA                                                                    >  SRR4302535.3824215/1‑94 (MQ=60)
                          CCAGGTCAGCGAGGAACGGCGCTTCCGGGCGAGTCAGCACAAACTGAACGGTGTTGTCGTCCACCCTTTTCACTTCACTGATCAGCTCTGGCAA                                                                   >  SRR4302535.2352431/1‑94 (MQ=60)
                          CCAGGTCAGCGAGGAACGGCGCTTCCAGGCGAGTCAGCACAAACTGAACGGTGTTGTCGTCCACCCTTTTCACTTCACTGATCAGCTCTGGCAA                                                                   <  SRR4302535.1919111/94‑1 (MQ=60)
                           CAGGTCAGCGAGGAACGGCGCTTCCGGGCGAGTCAGCACAAACTGAACGGTGTTGTCGTCCACCCTTTTCACTTCACTGATCAGCTCTGGCAAG                                                                  <  SRR4302535.1539575/94‑1 (MQ=60)
                            AGGTCAGCGAGGAACGGCGCTTCCGGGCGAGTCAGCACAAACTGAACGGTGTTGTCGTCCACCCTTTTCACTTCACTGATCAGCTCTGGCAAGC                                                                 >  SRR4302535.2821976/1‑94 (MQ=60)
                            AGGTCAGCGAGGAACGGCGCTTCCGGGCGAGTCAGCACAAACTGAACGGTGTTGTCGTCCACCCTTTTCACTTCACTGATCAGCTCTGGCAAGC                                                                 >  SRR4302535.3458855/1‑94 (MQ=60)
                             GGTCAGCGAGGAACGGCGCTTCCGGGCGAGTCAGCACAAACTGAACGGTGTTGTCGTCCACCCTTTTCACTTCACTGATCAGCTCTGGCAAGCC                                                                >  SRR4302535.3058584/1‑94 (MQ=60)
                                CAGCGAGGAACGGCGCTTCCGGGCGAGTCAGCACAAACTGAACGGTGTTGTCGTCCACCTTTTTCACTTCACTGATCAGCTCTGGCAAGCCCAT                                                             <  SRR4302535.1094551/94‑1 (MQ=60)
                                CAGCGAGGAACGGCGCTTCCGGGCGAGTCAGCACAAACTGAACGGTGTTGTCGTCCACCCTTTTCACTTCCCTGATCAGCTCTGGCAAGCCCAT                                                             <  SRR4302535.2746310/94‑1 (MQ=60)
                                 AGCGAGGAACGGGGCTTCCGGGCGAGTCAGCACAAACTGAACGGTGTTGTCGTCCACCCTTTTCACTTCACTGATCAGCTCTGGCAAGCCCATG                                                            >  SRR4302535.2762519/1‑94 (MQ=60)
                                 AGCGAGGAACGGCGCTTCCGGGCGAGTCAGCACAAACTGAACGGTGTTGTCGTCCACCCTTTTCACTTCACTGATCAGCTCTGGCAAGCCCATG                                                            <  SRR4302535.2781417/94‑1 (MQ=60)
                                 AGCGAGGAACGGCGCTTCCGGGCGAGTCAGCACAAACTGAACGGTGTTGTCGTCCACCCTTTTCACTTCACTGATCAGCTCTGGCAAGCCCATG                                                            >  SRR4302535.478295/1‑94 (MQ=60)
                                 AGCGAGGAACGGCGCTTCCGGGCGAGTCAGCACAAACTGAACGGTGTTGTCGTCCACCCTTTTCACTTCACTGATCAGCTCTGGCAAGCCCATG                                                            <  SRR4302535.3660826/94‑1 (MQ=60)
                                  GCGAGGAACGGCGCTTCCGGGCGAGTCAGCACAAACTGAACGGTGTTGTCGTCCACCCTTTTCACTTCACTGATCAGCTCTGGCAAGCCCATGC                                                           >  SRR4302535.4454761/1‑94 (MQ=60)
                                   CGAGGAACGGCGCTTCCGGGCGAGTCAGCACAAACTGAACGGTGTTGTCGTCCACCCTTTTCACTTCACTGATCAGCTCTGGCAAGCCCATGCC                                                          <  SRR4302535.3444840/94‑1 (MQ=60)
                                    GAGGAACGGCGCTTCCGGGCGAGTCAGCACAAACTGAACGGTGTTGTCGTCCACCCTTTTCACTTCACTGATCAGCTCTGGCAAGCCCATGCCT                                                         <  SRR4302535.2650565/94‑1 (MQ=60)
                                    GAGGAACGGCGCTTCCGGGCGAGTCAGCACAAACTGAACGGTGTTGTCGTCCACCCTTTTCACTTCACTGATCAGCTCTGGCAAGCCCATGCCT                                                         <  SRR4302535.4492879/94‑1 (MQ=60)
                                      GGAACGGCGCTTCCGGGCGAGTCAGCACAAACTGAACGGTGTTGTCGTCCACCCTTTTCACTTCACTGATCAGCTCTGGCAAGCCCATGCCTTC                                                       <  SRR4302535.2783070/94‑1 (MQ=60)
                                        AACGGCGCTTCCGGGCGAGTCAGCACAAACTGAACGGTGTTGTCGTCCACCCTTTTCACTTCACTGATCAGCTCTGGCAAGCCCATGCCTTCGA                                                     >  SRR4302535.3303466/1‑94 (MQ=60)
                                         ACGGCGCTTCCGGGCGAGTCAGCACAACCTGAACGGTATTGTCGTCCACCCTTTTCACTTCACTGATCAGCCCTGGCAAGCCCATGCCTTCGAA                                                    <  SRR4302535.2346842/94‑1 (MQ=60)
                                              GCTTCCGGGCGAGTCAGCACAAACTGAACGGTGTTGTCGTCCACCCTTTTCACTTCACTGATCAGCTCTGGCAAGCCCATGCCTTCGAAGTATT                                               <  SRR4302535.719025/94‑1 (MQ=60)
                                                TTCCGGGCGAGTCAGCACAAACTGAACGGTGTTGTCGTCCACCCTTTTCACTTCACTGATCAGCTCTGGCAAGCCCATGCCTTCGAAGTATTCG                                             <  SRR4302535.2392276/94‑1 (MQ=60)
                                                 TCCGGGCGAGTCATCACAAACTGAACGGTGTTGTCGTCCACCCTTTTCACTTCACTGATCAGCTCTGGCAAGCCCATGCCTTCGAAGTATTCGT                                            <  SRR4302535.995230/94‑1 (MQ=60)
                                                 TCCGGGCGAGTCAGCACAAACTGAACGGTGTTGTCGTCCACCCTTTTCACTTCACTGATCAGCTCTGGCAAGCCCATGCCTTCGAAGTATTCGT                                            <  SRR4302535.2987161/94‑1 (MQ=60)
                                                 TCCGGGCGAGTCAGCACAAACTGAACGGTGTTGTCGTCCACCCTTTTCACTTCACTGATCAGCTCTGGCAAGCCCATGCCTTCGAAGTATTCGT                                            >  SRR4302535.129266/1‑94 (MQ=60)
                                                 TCCGGGCGAGTCAGCACAAACTGAACGGTGTTGTCGTCCACCCTTTTCACTTCACTGATCAGCTCTGGCAAGCCCATGCCTTCGAAGTATTCGT                                            <  SRR4302535.2120931/94‑1 (MQ=60)
                                                   CGGGCGAGTCAGCACAAACTGAACGGTGTTGTCGTCCACCCTTTTCACTTCACTGATCAGCTCTGGCAAGCCCATGCCTTCGAAGTATTCGTAG                                          >  SRR4302535.567155/1‑94 (MQ=60)
                                                   CGGGCGAGTCAGCACAAACTGAACGGTGTTGTCGTCCACCCTTTTCACTTCACTGATCAGCTCTGGAAAGCCCATGCCTTCGAAGTATTCGTAG                                          >  SRR4302535.3131934/1‑94 (MQ=60)
                                                    GGGCGAGTCAGCACAAACTGAACGGTGTTGTCGTCCACCCTTTTCACTTCACTGATCAGCTCTGGCAAGCCCATGCCTTCGAAGTATTCGTAGC                                         <  SRR4302535.3004480/94‑1 (MQ=60)
                                                      GCGAGTCAGCACAAACTGAACGGTGTTGTCGTCCACCCTTTTCACTTCACTGATCAGCTCTGGCAAGCCCATGCCTTCGAAGTATTCGTAGCTG                                       <  SRR4302535.727817/94‑1 (MQ=60)
                                                        GAGTCAGCACAAACTGAACGGTGTTGTCGTCCACCCTTTTCACTTCACTGATCAGCTCTGGCAAGCCCATGCCTTCGCAGTATTCGTAGCTGCC                                     >  SRR4302535.1736680/1‑94 (MQ=60)
                                                        GAGTCAGCACAAACTGAACGGTGTTGTCGTCCACCCTTTTCACTTCACTGATCAGCTCTGGCAAGCCCATGCCTTCGAAGTATTCGTAGCTGCC                                     >  SRR4302535.715438/1‑94 (MQ=60)
                                                        GAGTCAGCACAAACTGAACGGTGTTGTCGTCCACCCTTTTCACTTCACTGATCAGCTCTGGAAAGCCCATGCCTTCGAAGTATTCGTAGCTGCC                                     >  SRR4302535.1036263/1‑94 (MQ=60)
                                                         AGTCAGCACAAACTGAACGGTGTTGTCGTCCACCCTTTTCACTTCACTGATCAGCTCTGGCAAGCCCATGCCTTCGAAGTATTCGTAGCTGCCG                                    >  SRR4302535.699652/1‑94 (MQ=60)
                                                         AGTCAGCACAAACTGAACGGTGTTGTCGTCCACCCTTTTCACTTCACTGATCAGCTCTGGCAAGCCCATGCCTTCGAAGTATTCGTAGCTGCCG                                    >  SRR4302535.2670675/1‑94 (MQ=60)
                                                          GTCAGCACAAACTGAACGGTGTTGTCGTCCACCCTTTTCACTTCACTGATCAGCTCTGGCAAGCCCATGCCTTCGAAGTATTCGTAGCTGCCGC                                   >  SRR4302535.583015/1‑94 (MQ=60)
                                                          GTCAGCACAAACTGAACGGTGTTGTCGTCCACCCTTTTCACTTCACTGATCAGCTCTGGCAAGCCCATGCCTTCGAAGTATTCGTAGCTGCCGC                                   >  SRR4302535.2748098/1‑94 (MQ=60)
                                                             AGCACAAACTGAACGGTGTTGTCGTCCACCCTTTTCACTTCACTGATCAGCTCTGGCAAGCCCATGCCTTCGAAGTATTCGTAGCTGCCGCCAG                                >  SRR4302535.2085015/1‑94 (MQ=60)
                                                               CACAAACTGAACGGTGTTGTCGTCCACCCTTTTCACTTCACTGATCAGCTCTGGCAAGCCCATGCCTTCGAAGTATTCGTAGCTGCCGCCAGAA                              >  SRR4302535.1634529/1‑94 (MQ=60)
                                                               CACAAACTGAACGGTGTTGTCGTCCACCCTTTTCACTTCACTGATCAGCTCTGGCAAGCCCATGCCTTCGAAGTATTCGTAGCTGCCGCCAGAA                              >  SRR4302535.2697115/1‑94 (MQ=60)
                                                               CACAAACTGAACGGTGTTGTCGTCCACCCTTTTCACTTCACTGATCAGCTCTGGCAAGCCCATGCCTTCGAAGTATTCGTAGCTGCCGCCAGAA                              >  SRR4302535.581555/1‑94 (MQ=60)
                                                                 CAAACTGAACGGTGTTGTCGTCCACCCTTTTCACTTCACTGATCAGCTCTGGCAAGCCCATGCCTTCGAAGTATTCGTAGCTGCCGCCAGAAAC                            >  SRR4302535.3234346/1‑94 (MQ=60)
                                                                 CAAACTGAACGGTGTTGTCGTCCACCCTTTTCACTTCACTGATCAGCTCTGGCAAGCCCATGCCTTCGAAGTATTCGTAGCTGCCGCCAGAAAC                            >  SRR4302535.2349660/1‑94 (MQ=60)
                                                                  AAACTGAACGGTGTTGTCGTCCACCCTTTTCACTTCACTGATCAGCTCTGGCAAGCCCATGCCTTCGAAGTATTCGTAGCTGCCGCCAGAAACT                           <  SRR4302535.1574483/94‑1 (MQ=60)
                                                                    ACTGAACGGTGTTGTCGTCCACCCTTTTCACTTCACTGATCAGCTCTGGCAAGCCCATGCCTTCGAAGTATTCGTAGCTGCCGCCAGAAACTTT                         <  SRR4302535.2836208/94‑1 (MQ=60)
                                                                      TGAACGGTGTTGTCGTCCACCCTTTTCACTTCACTGATCAGCTCTGGCAAGCCCATGCCTTCGAAGTATTCGTAGCTGCCGCCAGAAACTTTAT                       <  SRR4302535.208026/94‑1 (MQ=60)
                                                                      TGAACGGTGTTGTCGTCCACCCTTTTCACTTCACTGATCAGCTCTGGCAAGCCCATGCCTTCGAAGTATTCGTAGCTGCCGCCAGAAACTTTAT                       <  SRR4302535.4627547/94‑1 (MQ=60)
                                                                       GAACGGTGTTGTCGTCCACCCTTTTCACTTCACTGATCAGCTCTGGCAAGCCCATGCCTTCGAAGTATTCGTAGCTGCCGCCAGAAACTTTATG                      <  SRR4302535.3081365/94‑1 (MQ=60)
                                                                       GAACGGTGTTGTCGTCCACCCTTTTCACTTCACTGATCAGCTCTGGCAAGCCCATGCCTTCGAAGTATTCGTAGCTGCCGCCAGAAACTTTATG                      <  SRR4302535.1313466/94‑1 (MQ=60)
                                                                       GAACGGTGTTGTCGTCCACCCTTTTCACTTCACTGATCAGCTCTGGCAAGCCCATGCCTTCGAAGTATTCGTAGCTGCCGCCAGAAACTTTATG                      >  SRR4302535.1897677/1‑94 (MQ=60)
                                                                        AACGGTGTTGTCGTCCACCCTTTTCACTTCACTGATCAGCTCTGGCAAGCCCATGCCTTCGAAGTATTCGTAGCTGCCGCCAGAAACTTTATGG                     <  SRR4302535.396977/94‑1 (MQ=60)
                                                                        AACGGTGTTGTCGTCCACCCTTTTCACTTCACTGATCAGCTCTGGCAAGCCCATGCCTTCGAAGTATTCGTAGCTGCCGCCAGAAACTTTATGG                     <  SRR4302535.1685971/94‑1 (MQ=60)
                                                                         ACGGTGTTGTCGTCCACCCTTTTCACTTCACTGATCAGCTCTGGCAAGCCCATGCCTTCGAAGTATTCGTAGCTGCCGCCAGAAACTTTATGGT                    <  SRR4302535.3250608/94‑1 (MQ=60)
                                                                           GGTGTTGTCGTCCACCCTTTTCACTTCACTGATCAGCTCTGGCAAGCCCATGCCTTCGAAGTATTCGTAGCTGCCGCCAGAAACTTTATGGTAC                  >  SRR4302535.2461756/1‑94 (MQ=60)
                                                                           GGTGTTGTCGTCCACCCTTTTCACTTCACTGATCAGCTCTGGCAAGCCCATGCCTTCGAAGTATTCGTAGCTGCCGCCAGAAACTTTATGGTAC                  <  SRR4302535.785990/94‑1 (MQ=60)
                                                                           GGTGTTGTCGTCCACCCTTTTCACTTCACTGATCAGCTCTGGCAAGCCCATGCCTTCGAAGTATTCGTAGCTGCCGCCAGAAACTTTATGGTAC                  <  SRR4302535.409736/94‑1 (MQ=60)
                                                                           GGTGTTGTCGTCCACCCTTTTCACTTCACTGATCAGCTCTGGCAAGCCCATGCCTTCGAAGTATTCGTAGCTG‑CGCCAGAAACTTTATGGTACG                 >  SRR4302535.2707549/1‑94 (MQ=60)
                                                                            GTGTTGTCGTCCACCCTTTTCGCTTCACTGATCAGCTCTGGCAAGCCCATGCCTTCGAAGTATTCGTAGCTGCCGCCAGAAACTTTATGGTACG                 <  SRR4302535.1557123/94‑1 (MQ=60)
                                                                            GTGTTGTCGTCCACCCTTTTCACTTCACTGATCAGCTCTGGCAAGCCCATGCCTTCGAAGTATTCGTAGCTGCCGCCAGAAACTTTATGGTACG                 <  SRR4302535.4064442/94‑1 (MQ=60)
                                                                             TGTTGTCGTCCACCCTTTTCACTTCACTGATCAGCTCTGGCAAGCCCATGCCTTCGAAGTATTCGTAGCTGCCGCCAGAAACTTTATGGTACGG                >  SRR4302535.4149392/1‑94 (MQ=60)
                                                                             TGTTGTCGTCCACCCTTTTCACTTCACTGATCAGCTCTGGCAAGCCCATGCCTTCGAAGTATTCGTAGCTGCCGCCAGAAACTTTATGGTACGG                <  SRR4302535.2716848/94‑1 (MQ=60)
                                                                              GTTGTCGTCCACCCTTTTCACTTCACTGATCAGCTCTGGCAAGCCCATGCCTTCGAAGTATTCGTAGCTGCCGCCAGAAACTTTATGGTACGGG               <  SRR4302535.565207/94‑1 (MQ=60)
                                                                               TTGTCGTCCACCCTTTTCACTTCACTGATCAGCTCTGGCAAGCCCATGCCTTCGAAGTATTCGTAGCTGCCGCCAGAAACTTTATGGTACGGGT              <  SRR4302535.4461590/94‑1 (MQ=60)
                                                                               TTGTCGTCCACCCTTTTCACTTCACTGATCAGCTCTGGCAAGCCCATGCCTTCGAAGTATTCGTAGCTGCCGCCAGAAACTTTATGGTACGGGT              <  SRR4302535.4416687/94‑1 (MQ=60)
                                                                                TGTCGTCCACCCTTTTCACTTCACTGATCAGCTCTGGCAAGCCCATGCCTTCGAAGTATTCGTAGCTGCCGCCAGAAACTTTATGGTACGGGTT             <  SRR4302535.1348969/94‑1 (MQ=60)
                                                                                TGTCGTCCACCCTTTTCACTTCACTGATCAGCTCTGGCAAGCCCATGCCTTCGAAGTATTCGTAGCTGCCGCCAGAAACTTTATGGTACGGGCT             >  SRR4302535.670576/1‑94 (MQ=60)
                                                                                TGTCGTCCACCCTTTTCACTTCACTGATCAGCTCTGGCAAGCCCATGCCTCCGAAGTATTCGTAGCTGCCGCCAGAAACTTTATGGTACGGGTT             <  SRR4302535.2527962/94‑1 (MQ=60)
                                                                                    GTCCACCCTTTTCACTTCACTGATCAGCTCTGGCAAGCCCATGCCTTCGAAGTATTCGTAGCTGCCGCCAGAAACTTTATGGTACGGGTTTTGC         >  SRR4302535.3303626/1‑94 (MQ=60)
                                                                                     TCCACCCTCTTCACTTCACTGATCAGCTCTGGCAAGCCCATGCCTTCGAAGTATTCGTAGCTGCCGCCAGAAACTTTATGGTACGGGTTTTGCG        <  SRR4302535.2012875/94‑1 (MQ=60)
                                                                                      CCACCCTTTTCACTTCACTGATCAGCTCTGGCAAGCCCATGCCTTCGAAGTATTCGTAGCTGCCGCCAGAAACTTTATGGTACGGGTTTTGCGC       <  SRR4302535.3814943/94‑1 (MQ=60)
                                                                                       CACCCTTTTCACTTCACTGATCAGCTCTGGCAAGCCCATGCCTTCGAAGTATTCGTAGCTGCCGCCAGAAACTTTATGGTACGGGTTTTGCGCG      >  SRR4302535.501824/1‑94 (MQ=60)
                                                                                       CACCCTTTTCACTTCACTGATCAGCTCTGGCAAGCCCATGCCTTCGAAGTATTCGTAGCTGCCGCCAGAAACTTTATGGTACGGGTTTTGCGCG      <  SRR4302535.886323/94‑1 (MQ=60)
                                                                                        ACCCTTTTCACTTCACTGATCAGCTCTGGCAAGCCCATGCCTTCGAAGTATTCGTAGCTGCCGCCAGAAACTTTATGGTACGGGTTTTGCGCGT     >  SRR4302535.3021891/1‑94 (MQ=60)
                                                                                        ACCCTTTTCACTTCACTGATCAGCTCTGGCAAGCCCATGCCTTCGAAGTATTCGTAGCTGCCGCCAGAAACTTTATGGTACGGGTTTTGCGCGT     >  SRR4302535.575428/1‑94 (MQ=60)
                                                                                        ACCCTTTTCACTTCACTGATCAGCTCTGGCAAGCCCATGCCTTCGAAGTATTCGTAGCTGCCGCCAGAAACTTTATGGTACGGGTTTTGCGCGT     >  SRR4302535.4491337/1‑94 (MQ=60)
                                                                                        ACCCTTTTCACTTCACTGATCAGCTCTGGCAAGCCCATGCCTTCGAAGTATTCGTAGCTGCCGCCAGAAACTTTATGGTACGGGTTTTGCGCGT     >  SRR4302535.3592142/1‑94 (MQ=60)
                                                                                        ACCCTTTTCACTTCACTGATCAGCTCTGGCAAGCCCATGCCTTCGAAGTATTCGTAGCTGCCGCCAGAAACTTTATGGTACGGGTTTTGCGCGT     >  SRR4302535.3263212/1‑94 (MQ=60)
                                                                                        ACCCTTTTCAATTCACTGATCAGCTCTGGCAAGCCCATGCCTTCGAAGTATTCGTAGCTGCCGCCAGAAACTTTATGGTACGGGTTTTGCGCGT     >  SRR4302535.2900903/1‑94 (MQ=60)
                                                                                         CCCTTTTCACTTCACTGATCAGCTCTGGCAAGCCCATGCCTTCGAAGTATTCGTAGCTGCCGCCAGAAACTTTATGGTACGGGTTTTGCGCGTT    >  SRR4302535.70235/1‑94 (MQ=60)
                                                                                          CCTTTTCACTTCACTGATCAGCTCTGGCAAGCCCATGCCTTCGAAGTATTCGTAGCTGCCGCCAGAAACTTTATGGTACGGGTTTTGCGCGTTT   >  SRR4302535.1000895/1‑94 (MQ=60)
                                                                                           CTTTTCACTTCACTGATCAGCGCTCGCAAGCCCATGCCTTCGAAGTCTTCGTAGCTGCCGCCAGAAACTTTATAGTACGGGTTTTGCGCGTTTT  >  SRR4302535.2311264/1‑94 (MQ=60)
                                                                                           |                                                                                             
TGACAGAATAGAGGCGAAGTCCATTGCCAGGTCAGCGAGGAACGGCGCTTCCGGGCGAGTCAGCACAAACTGAACGGTGTTGTCGTCCACCTTTTTCACTTCACTGATCAGCTCTGGCAAGCCCATGCCTTCGAAGTATTCGTAGCTGCCGCCAGAAACTTTATGGTACGGGTTTTGCGCGTTTT  >  NC_000913/3704774‑3704958

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑