Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 1696 279.3 15874187 97.8% 15524954 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,704,865 T→C K157R (AAG→AGG)  dppA ← dipeptide transport protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,704,8650TC99.8% 1293.2 / ‑5.1 409K157R (AAG→AGG) dppAdipeptide transport protein
Reads supporting (aligned to +/- strand):  ref base T (1/0);  new base C (199/209);  total (200/209)
Fisher's exact test for biased strand distribution p-value = 4.89e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.77e-01

Only 100 of 424 total aligned reads displayed.

GACAGAATAGAGGCGAAGTCCATTGCCAGGTCAGCGAGGAACGGCGCTTCCGGGCGAGTCAGCACAAACTGAACGGTGTTGTCGTCCACCTTTTTCACTTCACTGATCAGCTCTGGCAAGCCCATGCCTTCGAAGTATTCGTAGCTGCCGCCAGAAACTTTATGGTACGGGTTTTGCGCG  >  NC_000913/3704775‑3704954
                                                                                          |                                                                                         
gACAGAATAGAGGCGAAGTCCATTGCCAGGTCAGCGAGGAACGGCGCTTCCGGGCGAGTCAGCACAAACTGAACGGTGTTGTCGTCCACCctt                                                                                         >  1:5507015/1‑93 (MQ=255)
 aCAGAATAGAGGCGAAGTCCATTGCCAGGTCAGCGAGGAACGGCGCTTCCGGGCGAGTCAGCACAAACTGAACGGTGTTGTCGTCCACCcttt                                                                                        >  1:3412447/1‑93 (MQ=255)
 aCAGAATAGAGGCGAAGTCCATTGCCAGGTCAGCGAGGAACGGCGCTTCCGGGCGAGTCAGCACAAACTGAACGGTGTTGTCGTCCACCcttt                                                                                        <  1:2538506/93‑1 (MQ=255)
   aGAATAGAGGCGAAGTCCATTGCCAGGTCAGCGAGGAACGGCGCTTCCGGGCGAGTCAGCACAAACTGAACGGTGTTGTCGTCCACCCTTttc                                                                                      >  1:4599941/1‑93 (MQ=255)
    gAATAGAGGCGAAGTCCATTGCCAGGTCAGCGAGGAACGGCGCTTCCGGGCGAGTCAGCACAAACTGAACGGTGTTGTCGTCCACCCTTttca                                                                                     >  2:3352762/1‑93 (MQ=255)
    gAATAGAGGCGAAGTCCATTGCCAGGTCAGCGAGGAACGGCGCTTCCGGGCGAGTCAGCACAAACTGAACGGTGTTGTCGTCCACCCTTttca                                                                                     >  1:4395552/1‑93 (MQ=255)
     aaTAGAGGCGAAGTCCATTGCCAGGTCAGCGAGGAACGGCGCTTCCGGGCGAGTCAGCACAAACTGAACGGTGTTGTCGTCCACCCTTttcac                                                                                    <  1:7542284/93‑1 (MQ=255)
      aTAGAGGCGAAGTCCATTGCCAGGTCAGCGAGGAACGGCGCTTCCGGGCGAGTCAGCACAAACTGAACGGTGTTGTCGTCCACCCTTttcact                                                                                   >  1:1438664/1‑93 (MQ=255)
        agagGCGAAGTCCATTGCCAGGTCAGCGAGGAACGGCGCTTCCGGGCGAGTCAGCACAAACTGAACGGTGTTGTCGTCCACCCTTttcacttc                                                                                 >  1:3910208/1‑93 (MQ=255)
         gagGCGAAGTCCATTGCCAGGTCAGCGAGGAACGGCGCTTCCGGGCGAGTCAGCACAAACTGAACGGTGTTGTCGTCCACCCTTttcccttca                                                                                >  2:1600724/1‑93 (MQ=255)
         gagGCGAAGTCCATTGCCAGGTCAGCGAGGAACGGCGCTTCCGGGCGAGTCAGCACAAACTGAACGGTGTTGTCGTCCACCCTTttcacttca                                                                                >  1:2311919/1‑93 (MQ=255)
           ggCGAAGTCCATTGCCAGGTCAGCGAGGAACGGCGCTTCCGGGCGAGTCAGCACAAACTGAACGGTGTTGTCGTCCACCCTTTtcacttcact                                                                              >  1:954828/1‑93 (MQ=255)
           ggCGAAGTCCATTGCCAGGTCAGCGAGGAACGGCGCTTCCGGGCGAGTCAGCACAAACTGAACGGTGTTGTCGTCCACCCTTTtcacttcact                                                                              <  1:5116135/93‑1 (MQ=255)
            gCGAAGTCCCTTGCCAGGTCAGCGAGGAACGGCGCTTCCGGGCGAGTCAGCACAAACTGAACGGTGTTGTCGTCCACCCTTTTCACTTCACTg                                                                             <  2:6579670/93‑1 (MQ=255)
            gCGAAGTCCATTGCCAGGTCAGCGAGGAACGGCGCTTCCGGGCGAGTCAGCACAAACTGAACGGTGTTGTCGTCCACCCTTTTCACTTCACTg                                                                             >  2:3262838/1‑93 (MQ=255)
            gCGAAGTCCATTGCCAGGTCAGCGAGGAACGGCGCTTCCGGGCGAGTCAGCACAAACTGAACGGTGTTGTCGTCCACCCTTTTCACTTCACTg                                                                             <  1:6997593/93‑1 (MQ=255)
            gCGAAGTCCATTGCCAGGTCAGCGAGGAACGGCGCTTCCGGGCGAGTCAGCACAAACTGAACGGTGTTGTCGTCCACCCTTTTCACTTCACTg                                                                             <  1:157051/93‑1 (MQ=255)
             cGAAGTCCATTGCCAGGTCAGCGAGGAACGGCGCTTCCGGGCGAGTCAGCACAAACTGAACGGTGTTGTCGTCCACCCTTTTCACTTCACTGa                                                                            <  1:7616260/93‑1 (MQ=255)
             cGAAGTCCATTGCCAGGTCAGCGAGGAACGGCGCTTCCGGGCGAGTCAGCACAAACTGAACGGTGTTGTCGTCCACCCTTTTCACTTCACTGa                                                                            <  1:3183059/93‑1 (MQ=255)
               aaGTCCATTGCCAGGTCAGCGAGGAACGGCGCTTCCGGGCGAGTCAGCACAAACTGAACGGTGTTGTCGTCCACCCTTTTCACTTCACTGATc                                                                          >  2:5359501/1‑93 (MQ=255)
                aGTCCATTGCCAGGTCAGCGAGGAACGGCGCTTCCGGGCGAGTCAGCACAAACTGAACGGTGTTGTCGTCCACCCTTTTCACTTCACTGATCa                                                                         >  2:5037330/1‑93 (MQ=255)
                 gTCCATTGCCAGGTCAGCGAGGAACGGCGCTTCCGGGCGAGTCAGCACAAACTGAACGGTGTTGTCGTCCACCCTTTTCACTTCACTGATCAg                                                                        >  2:7738245/1‑93 (MQ=255)
                 gTCCATTGCCAGGTCAGCGAGGAACGGCGCTTCCGGGCGAGTCAGCACAAACTGAACGGTGTTGTCGTCCACCCTTTTCACTTCACTGATCAg                                                                        >  1:6420284/1‑93 (MQ=255)
                  tCCATTGCCAGGTCAGCGAGGAACGGCGCTTCCGGGCGAGTCAGCACAAACTGAACGGTGTTGTCGTCCACCCTTTTCACTTCACTGATCAGc                                                                       <  1:414543/93‑1 (MQ=255)
                    cATTGCCAGGTCAGCGAGGAACGGCGCTTCCGGGCGAGTCAGCACAAACTGAACGGTGTTGTCGTCCACCCTTTTCACTTCACTGATCAGctc                                                                     <  1:3271654/93‑1 (MQ=255)
                    cATTGCCAGGTCAGCGAGGAACGGCGCTTCCGGGCGAGTCAGCACAAACTGAACGGTGTTGTCGTCCACCCTTTTCACTTCACTGATCAGctc                                                                     <  2:6948056/93‑1 (MQ=255)
                     aTTGCCAGGTCAGCGAGGAACGGCGCTTCCGGGCGAGTCAGCACAAACTGAACGGTGTTGTCGTCCACCCTTTTCACTTCACTGATCAGctct                                                                    >  1:6088145/1‑93 (MQ=255)
                     aTTGCCAGGTCAGCGAGGAACGGCGCTTCCGGGCGAGTCAGCACAAACTGAACGGTGTTGTCGTCCACCCTTTTCACTTCACTGATCAGctct                                                                    <  1:2047838/93‑1 (MQ=255)
                     aTTGCCAGGTCAGCGAGGAACGGCGCTTCCGGGCGAGTCAGCACAAACTGAACGGTGTTGTCGTCCACCCTTTTCACTTCACTGATCAGctct                                                                    <  2:7284057/93‑1 (MQ=255)
                     aTTGCCAGGTCAGCGAGGAACGGCGCTTCCGGGCGAGTCAGCACAAACTGAACGGTGTTGTCGTCCACCCTTTTCACTTCACTGATCAGctct                                                                    >  1:4013865/1‑93 (MQ=255)
                      ttGCCAGGTCAGCGAGGAACGGCGCTTCCGGGCGAGTCAGCACAAACTGAACGGTGTTGTCGTCCACCCTTTTCACTTCACTGATCAGCTCTg                                                                   >  2:1678240/1‑93 (MQ=255)
                      ttGCCAGGTCAGCGAGGAACGGCGCTTCCGGGCGAGTCAGCACAAACTGAACGGTGTTGTCGTCCACCCTTTTCACTTCACTGATCAGCTCTg                                                                   >  1:1290449/1‑93 (MQ=255)
                      ttGCCAGGTCAGCGAGGAACGGCGCTTCCGGGCGAGTCAGCACAAACTGAACGGGGTTGTCGTCCACCCTTTTCACTTCACTGATCAGCTCTg                                                                   >  1:7734530/1‑93 (MQ=255)
                          cAGGTCAGCGAGGAACGGCGCTTCCGGGCGAGTCAGCACAAACTGAACGGTGTTGTCGTCCACCCTTTTCACTTCACTGATCAGCTCTGGCaa                                                               >  1:6612281/1‑93 (MQ=255)
                           aGGTCAGCGAGGAACGGCGCTTCCGGGCGAGTCAGCACAAACTGAACGGTGTTGTCGTCCACCCTTTTCACTTCACTGATCAGCTCTGGCAAg                                                              >  2:799963/1‑93 (MQ=255)
                             gTCAGCGAGGAACGGCGCTTCCGGGCGAGTCAGCACAAACTGAACGGTGTTGTCGTCCACCCTTTTCACTTCACTGATCAGCTCTGGCAAGcc                                                            >  2:4228057/1‑93 (MQ=255)
                               cAGCGAGGAACGGCGCTTCCGGGCGAGTCAGCACAAACTGAACGGTGTTGTCGTCCACCCTTTTCACTTCACTGATCAGCTCTGGCAAGCCCa                                                          <  2:5310723/93‑1 (MQ=255)
                                aGCGAGGAACGGCGCTTCCGGGCGAGTCAGCACAAACTGAACGGTGTTGTCGTCCACCCTTTTCACTTCACTGATCAGCTCTGGCAAGCCCAt                                                         >  1:3508388/1‑93 (MQ=255)
                                aGCGAGGAACGGCGCTTCCGGGCGAGTCAGCACAAACTGAACGGTGTTGTCGTCCACCCTTTTCACTTCACTGATCAGCTCTGGCAAGCCCAt                                                         <  2:7180203/93‑1 (MQ=255)
                                 gcgcGGAACGGCGCTTCCGGGCGAGTCAGCACAAACTGAACGGTGTTGTCGTCCACCCTTTTCACTTCACTGATCAGCTCTGGCAAGCCCATg                                                        <  2:2918392/93‑1 (MQ=255)
                                 gCGAGGAACGGCGCTTCCGGGCGAGTCAGCACAAACTGAACGGTGTTGTCGTCCACCCTTTTCACTTCACTGATCAGCTCTGGCAAGCCCATg                                                        >  2:4566805/1‑93 (MQ=255)
                                 gCGAGGAACGGCGCTTCCGGGCGAGTCAGCACAAACTGAACGGTGTTGTCGTCCACCCTTTTCACTTCACTGATCAGCTCTGGCAAGCCCATg                                                        <  1:2841572/93‑1 (MQ=255)
                                    aGGAACGGCGCTTCCGGGCGAGTCAGCACAAACTGAACGGTGTTGTCGTCCACCCTTTTCACTTCACTGATCAGCTCTGGCAAGCCCATGCCt                                                     >  1:6763292/1‑93 (MQ=255)
                                    aGGAACGGCGCTTCCGGGCGAGTCAGCACAAACTGAACGGTGTTGTCGTCCACCCTTTTCACTTCACTGATCAGCTCTGGCAAGCCCATGCCt                                                     >  2:4094235/1‑93 (MQ=255)
                                    aGGAACGGCGCTTCCGGGCGAGTCAGCACAAACTGAACGGTGTTGTCGTCCACCCATTTCACTTCACTGATCAGCTCTGGCAAGCCCATGCCt                                                     <  1:194189/93‑1 (MQ=255)
                                     ggAACGGCGCTTCCGGGCGAGTCAGCACAAACTGAACGGGGTTGTCGTCCACCCTTTTCACTTCACTGATCAGCTCTGGGAAGCCCATGCCtt                                                    >  2:1070741/1‑93 (MQ=255)
                                      taaCGGCGCATCCGGGCTAGTCAGCACAAACTGAACGGTGTTGTCGTCCTCCCTTTTCACTTCACTGATCAGCTCTGGCAAGCCCATGCCTTc                                                   >  2:7046900/2‑93 (MQ=255)
                                      gAACGGCGCTTCCGGGCGAGTCAGCACAAACTGAACGGTGTTGTCGTCCACCCTTTTCACTTCACTGATCGNCTCTGGCAAGCCCATGCCTTc                                                   >  1:5903062/1‑93 (MQ=255)
                                      gAACGGCGCTTCCGGGCGAGTCAGCACAAACTGAACGGTGTTGTCGTCCACCCTTTTCACTTCACTGATCAGCTCTGGCAAGCCCATGCCTTc                                                   <  2:6048830/93‑1 (MQ=255)
                                        aCGGCGCTTCCGGGCGAGTCAGCACAAACTGAACGGTGTTGTCGTCCACCCTTTTCACTTCACTGATCAGCTCTGGCAAGCCCATGCCTTCGa                                                 <  2:1156742/93‑1 (MQ=255)
                                        aCGGCGCTTCCGGGCGAGTCAGCACAAACTGAACGCTGTTGTCGTCCACCCTTTTCACTTCACTGATCAGCTCTGGCAAGCCCATGCCTTCGa                                                 >  2:6217941/1‑93 (MQ=255)
                                         cGGCGCTTCCGGGCGAGTCAGCACAAACTGAACGGTGTTGTCGTCCACCCTTTTCACTTCACTTATCAGCTCTGGCAAGCCCATGCCTTCGaa                                                <  1:6691646/93‑1 (MQ=255)
                                         cGGCGCTTCCGGGCGAGTCAGCACAAACTGAACGGTGTTGTCGTCCACCCTTTTCACTTCACTGATCAGCTCTGGCAAGCCCATGCCTTCGaa                                                <  1:5043271/93‑1 (MQ=255)
                                              cTTCCGGGCGAGTCAGCACAAACTGAACGGTGTTGTCGTCCACCCTTTTCACTTCACTGATCAGCTCTGGCAAGCCCATGCCTTCGAAGTAtt                                           >  2:2051335/1‑93 (MQ=255)
                                              cTTCCGGGCGAGTCAGCACAAACTGAACGGTGTTGTCGTCCACCCTTTTCACTTCACTGATCAGCTCTGGCAAGCCCATGCCTTCGAAGTAtt                                           <  1:1164758/93‑1 (MQ=255)
                                               ttCCGGGCGAGTCAGCACAAACTGAACGGTGTTGTCGTCCACCCTTTTCACTTCACTGATCAGCTCTGGCAAGCCCATGCCTTCGAAGTATTc                                          >  1:4780128/1‑93 (MQ=255)
                                                tCCGGGCGAGTCAGCACAAACTGAACGGTGTTGTCGTCCACCCTTTTCACTTCACTGATCAGCTCTGGCAAGCCCATGCCTTCGAAGTATTCg                                         >  1:3778632/1‑93 (MQ=255)
                                                tCCGGGCGAGTCAGCACAAACTGAACGGTGTTGTCGTCCACCCTTTTCACTTCACTGATCAGCTCTGGCAAGCCCATGCCTTCGAAGTATTCg                                         <  1:651933/93‑1 (MQ=255)
                                                 ccGGGCGAGTCAGCACAAACTGAACGGTGTTGTCGTCCACCCTTTTCACTTCACTGATCAGCTCTGGCAAGCCCATGCCTTCGAAGTATTCGt                                        <  2:5211933/93‑1 (MQ=255)
                                                   gggCGAGTCAGCACAAACTGAACGGTGTTGTCGTCCACCCTTTTCACTTCACTGATCAGCTCTGGCAAGCCCATGCCTTCGAAGTATTCGTAg                                      <  2:1427777/93‑1 (MQ=255)
                                                      cGAGTCAGCACAAACTGAACGGTGTTGTCGTCCACCCTTTTCACTTCACTGATCAGCTCTGGCAAGCCCATGCCTTCGAAGTATTCGTAGCTg                                   <  1:1045309/93‑1 (MQ=255)
                                                       gAGTCAGCACAAACTGAACGGTGTTGTCGTCCACCCTTTTCACTTCACTGATCAGCTCTGGCAAGCCCATGCCTTCGAAGTATTCGTAGCTgc                                  >  1:4851174/1‑93 (MQ=255)
                                                        aGTCAGCACAAACTGAACGGTGTTGTCGTCCACCCTTTTCACTTCACTGATCAGCTCTGGCAAGCCCATGCCTTCGAAGTATTCGTAGCTgcc                                 >  2:3087436/1‑93 (MQ=255)
                                                           cAGCACAAACTGAACGGTGTTGTCGTCCACCCTTTTCACTTCACTGATCAGCTCTGGCAAGCCCATGCCTTCGAAGTATTCGTAGCTgccgcc                              <  1:1664438/93‑1 (MQ=255)
                                                            aGCACAAACTGAACGGTGTTGTCGTCCACCCTTTTCACTTCACTGATCAGCTCTGGCAAGCCCATGCCTTCGAAGTATTCGTAGCTGCCGCCa                             >  2:2248534/1‑93 (MQ=255)
                                                                 aaaCTGAACGGTGTTGTCGTCCACCCTTTTCACTTCACTGATCAGCTCTGGCAAGCCCATGCCTTCGAAGTATTCGTAGCTGCCGCCAGAAAc                        <  2:7499356/93‑1 (MQ=255)
                                                                 aaaCTGAACGGTGTTGTCGTCCACCCTTTTCACTTCACTGATCAGCTCTGGCAAGCCCATGCCTTCGAAGTATTCGTAGCTGCCGCCAGAAAc                        <  2:3987845/93‑1 (MQ=255)
                                                                  aaCTGAACGGTGTTGTCGTCCACCCTTTTCACTTCACTGATCAGCTCTGGAAAGCCCATGCCTTCGAAGGATTCGTAGCTGCCGCCAGaacat                       >  2:2350153/1‑90 (MQ=255)
                                                                     tGAACGGTGTTGTCGTCCACCCTTTTCACTTCACTGATCAGCTCTGGCAAGCCCATGCCTTCGAAGTATTCGTAGCTGCCGCCAGAAACTTTa                    <  2:1299634/93‑1 (MQ=255)
                                                                     tGAACGGTGTTGTCGTCCACCCTTTTCACTTCACTGATCAGCTCTGGCAAGCCCATGCCTTCGAAGTATTCGTAGCTGCCGCCAGAAACTTTa                    <  1:4108385/93‑1 (MQ=255)
                                                                      gAACGGTGTTGTCGTCCACCCTTTTCACTTCACTGATCAGCTCTGGCAAGCCCATGCCTTCGAAGTATTCGTAGCTGCCGCCAGAAACTTTAt                   <  2:6731712/93‑1 (MQ=255)
                                                                       aaCGGTGTTGTCGTCCACCCTTTTCACTTCACTGATCAGCTCTGGCAAGCCCATGCCTTCGAAGTATTCGTAGCTGCCGCCAGAAACTTTATg                  <  2:1886125/93‑1 (MQ=255)
                                                                       aaCGGTGTTGTCGTCCACCCTTTTCACTTCACTGATCAGCTCTGGCAAGCCCATGCCTTCGAAGTATTCGTAGCTGCCGCCAGAAACTTTATg                  <  2:4749364/93‑1 (MQ=255)
                                                                        aCGGTGTTGTCGTCCACCCTTTTCACTTCACTGATCAGCTCTGGCAAGCCCATGCCTTCGAAGTATTCGTAGCTGCCGCCAGAAACTTTATgg                 >  2:6837040/1‑93 (MQ=255)
                                                                         cGGTGTTGTCGTCCACCCTTTTCACTTCACTGATCAGCTCTGGCGAGCCCATGCCTTCGAAGTATTCGTAGCTGCCGCCAGAAACTTTATGGt                <  1:7409432/93‑1 (MQ=255)
                                                                         cGGTGTTGTCGTCCACCCTTTTCACTTCACTGATCAGCTCTGGCAAGCCCATGCCTTCGAAGTATTCGTAGCTGCCGCCAGAAACTTTATGGt                <  2:271254/93‑1 (MQ=255)
                                                                          ggTGTTGTCGTCCACCCTTTTCACTTCACTGATCAGCTCTGGCAAGCCCATGCCTTCGAAGTATTCGTAGCTGCCGCCAGAAACTTTATGGTa               <  2:4363196/93‑1 (MQ=255)
                                                                          ggTGTTGTCGTCCACCCTTTTCACTTCACTGATCAGCTCTGGCAAGCCCATGCCTTCGAAGTATTCGTAGCTGCCGCCAGAAACTTTATGGTa               <  1:3044058/93‑1 (MQ=255)
                                                                           gtgtTGTCGTCCACCCTTTTCACTTCACTGATCAGCTCTGGCAAGCCCATGCCTTCGAAGTATTCGTAGCTGCCGCCAGAAACTTTATGGTAc              <  1:5725580/93‑1 (MQ=255)
                                                                           gtgtTGTCGTCCACCCTTTTCACTTCACTGATCAGCTCTGGCAAGCCCATGCCTTCGAAGTATTCGTAGCTGCCGCCAGAAACTTTATGGTAc              <  2:1330313/93‑1 (MQ=255)
                                                                           gtgtTGTCGTCCACCCTTTTCACTTCACTGATCAGCTCTGGCAAGCCCATGCCTTCGAAGTATTCGTAGCTGCCGCCAGAAACTTTATGGTAc              <  1:3574217/93‑1 (MQ=255)
                                                                           gtgtTGTCGTCCACCCTTTTCACTTCACTGATCAGCTCTGGCAAGCCCATGCCTTCGAAGTATTCGTAGCTGCCGCCAGAAACTTTATGGTAc              <  2:6522445/93‑1 (MQ=255)
                                                                            tgttgtCGTCCACCCTTTTCACTTCACTGATCAGCTCTGGCAAGCCCATGCCTTCGAAGTATTCGTAGCTGCCGCCAGAAACTTTATGGTACg             >  1:7830410/1‑93 (MQ=255)
                                                                             gttgtCGTCCACCCTTTTCACTTCACTGATCAGCTCTGGCAAGCCCATGCCTTCGAAGTATTCGTAGCTGCCGCCAGAAACTTTATGGTACgg            >  2:5960272/1‑93 (MQ=255)
                                                                             gttgtCGTCCACCCTTTTCACTTCACTGATCAGCTCTGGCAAGCCCATGCCTTCGAAGTATTCGTAGCTGCCGCCAGAAACTTTATGGTACgg            <  1:5250375/93‑1 (MQ=255)
                                                                             gttgtCGTCCACCCTTTTCACTTCACTGATCAGCTCTGGCAAGCCCATGCCTTCGAAGTATTCGTAGCTGCCGCCAGAAACTTTATGGTACgg            >  1:4331541/1‑93 (MQ=255)
                                                                             gttgtCGGCCACCCTTTTCACTGCACTGATCAGCTCTTGCAAGCCCATGCCTTCGAAGTATTCGTAGCTGCCGCCAGAAACTTTATGGTACgg            <  2:6356490/93‑1 (MQ=255)
                                                                              ttgtCGTCCACCCTTTTCACTTCACTGATCAGCTCTGGCAAGCCCATGCCTTCGAAGTATTCGTAGCTGCCGCCAGAAACTTTATGGTACggg           <  1:5619585/93‑1 (MQ=255)
                                                                               tgtCGTCCACCCTTTTCACTTCACTGATCAGCTCTGGCAAGCCCATGCCTTCGAAGTATTCGTAGCTGCCGCCAGAAACTTTATGGTACGGGt          <  2:2477286/93‑1 (MQ=255)
                                                                               tgtCGTCCACCCTTTTCACTTCACTGATCAGCTCTGGCAAGCCCATGCCTTCGAAGTATTCGTAGCTGCCGCCAGAAACTTTATGGTACGGGt          <  1:6918738/93‑1 (MQ=255)
                                                                                 tcgtcCACCCTTTTCACTTCACTGATCAGCTCTGGCAAGCCCATGCCTTCGAAGTATTCGTAGCTGCCGCCAGAAACTTTATGGTACGGGttt        <  2:2990371/93‑1 (MQ=255)
                                                                                  cgtcCACCCTTTTCACTTCACTGATCAGCTCTGGCAAGCCCATGCCTTCGAAGTATTCGTAGCTGCCGCCAGAAACTTTATGGTACGGGtttt       <  2:3754265/93‑1 (MQ=255)
                                                                                   gtcCACCCTTTTCACTTCACTGATCAGCTCTGGCAAGCCCATGCCTTCGAAGTATTCGTAGCTGCCGCCAGAAACTTTATGGTACGGGTTTTg      >  1:1880575/1‑93 (MQ=255)
                                                                                     ccACCCTTTTCACTTCACTGATCAGCTCTGGCAAGCCCATGCCTTCGAAGTATTCGTAGCTGCCGCCAGAAACTTTATGGTACGGGTTTTgcg    >  2:2083667/1‑93 (MQ=255)
                                                                                     ccACCCTTTTCACTTCACTGATCAGCTCTGGCAAGCCCATGCCTTCGAAGTATTCGTAGCTGCCGCCAGAAACTTTATGGTACGGGTTTTgcg    >  1:1397680/1‑93 (MQ=255)
                                                                                     ccACCCTTTTCACTTCACTGATCAGCTCTGGCAAGCCCATGCCTTCGAAGTATTCGTAGCTGCCGCCAGAAACTTTATGGTACGGGTTTTgcg    >  2:7770668/1‑93 (MQ=255)
                                                                                      cACCCTTTTCACTTCACTGATCAGCTCTGGCAAGCCCATGCCTTCGAAGTATTCGTAGCTGCCGCCGGAAACTTTATGGTACGGGTGTTgcgc   >  1:6414486/1‑93 (MQ=255)
                                                                                      cACCCTTTTCACTTCACTGATCAGCTCTGGCAAGCCCATGCCTTCGAAGTATTCGTAGCTGCCGCCAGAAACTTTATGGGACGGGTTTTgcgg   >  1:2108669/1‑92 (MQ=255)
                                                                                       aCCCTTTTCACTTCACTGATCAGCTCTGGCAAGCCCATGCCTTCGAAGTATTCGTAGCTGCCGCCAGAAACTTTATGGTACGGGTTTTGcgcg  >  2:3507122/1‑93 (MQ=255)
                                                                                       aCCCTTTTCACTTCACTGATCAGCTCTGGCAAGCCCATGCCTTCGAAGTATTCGTAGCTGCCGCCAGAAACTTTATGGTACGGGTTTTGcgcg  >  2:5580737/1‑93 (MQ=255)
                                                                                          |                                                                                         
GACAGAATAGAGGCGAAGTCCATTGCCAGGTCAGCGAGGAACGGCGCTTCCGGGCGAGTCAGCACAAACTGAACGGTGTTGTCGTCCACCTTTTTCACTTCACTGATCAGCTCTGGCAAGCCCATGCCTTCGAAGTATTCGTAGCTGCCGCCAGAAACTTTATGGTACGGGTTTTGCGCG  >  NC_000913/3704775‑3704954

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output Only 200 of 237 total aligned reads displayed.
TTTGACAGAATAGAGGCGAAGTCCATTGCCAGGTCAGCGAGGAACGGCGCTTCCGGGCGAGTCAGCACAAACTGAACGGTGTTGTCGTCCACCTTTTTCACTTCACTGATCAGCTCTGGCAAGCCCATGCCTTCGAAGTATTCGTAGCTGCCGCCAGAAACTTTATGGTACGGGTTTTGCGCGTTTT  >  NC_000913/3704772‑3704958
                                                                                             |                                                                                             
TTTGACAGAATGGAGGCGAAGTCCATTGCCAGGTCAGCGAGTAACGGCGCTTCCGGGCGAGTGAGCACAACCTGACCGGTGTTGTCGTCCACCC                                                                                               <  SRR4302543.7292717/94‑1 (MQ=60)
TTTGACAGAATAGAGGCGAAGTCCATTGCCAGGTCAGCGAGGAACGGCGCTTCCGGGCGAGTCAGCACAAACTGAACGGTGTTGTCGTCCACCC                                                                                               <  SRR4302543.7292716/94‑1 (MQ=60)
 TTGACAGAATAGAGGCGAAGTCCATTGCCAGGTCAGCGAGGAACGGCGCTTCCGGGCGAGTCAGCACAAACTGAACGGTGTTGTCGTCCACCCT                                                                                              <  SRR4302543.7520593/94‑1 (MQ=60)
 TTGACAGAATAGAGGCGAAGTCCATTGCCAGGTCAGCGAGGAACGGCGCTTCCGGGCGAGTCAGCACAAACTGAACGGTGTTGTCGTCCACCCT                                                                                              <  SRR4302543.1818241/94‑1 (MQ=60)
 TTGACAGAATAGAGGCGAAGTCCATTGCCAGGTCAGCGAGGAACGGCGCTTCCGGGCGAGTCAGCACAAACTGAACGGTGTTGTCGTCCACCCT                                                                                              <  SRR4302543.1726251/94‑1 (MQ=60)
 TTGACAGAATAGAGGCGAAGTCCATTGCCAGGTCAGCGAGGAACGGCGCTTCCGGGCGAGTCAGCACAAACTGAACGGTGTTGTCGTCCACCCT                                                                                              >  SRR4302543.2975895/1‑94 (MQ=60)
  TGACAGAATAGAGGCGAAGTCCATTGCCAGGTCAGCGAGGAACGGCGCTTCCGGGCGAGTCAGCACAAACTGAACGGTGTTGTCGTCCACCCTT                                                                                             >  SRR4302543.598531/1‑94 (MQ=60)
  TGACAGAATAGAGGCGAAGTCCATTGCCAGGTCAGCGAGGAACGGCGCTTCCGGGCGAGTCAGCACAAACTGAACGGTGTTGTCGTCCACCCTT                                                                                             >  SRR4302543.5557013/1‑94 (MQ=60)
   GACAGAATAGAGGCGAAGTCCATTGCCAGGTCAGCGAGGAACGGCGCTTCCGGGCGAGTCAGCACAAACTGAACGGTGTTGTCGTCCACCCTTT                                                                                            <  SRR4302543.6579027/94‑1 (MQ=60)
   GACAGAATAGAGGCGAAGTCCATTGCCAGGTCAGCGAGGAACGGCGCTTCCGGGCGAGTCAGCACAAACTGAACGGTGTTGTCGTCCACCCTTT                                                                                            >  SRR4302543.3442997/1‑94 (MQ=60)
     CAGAATAGAGGCGAAGTCCATTGCCAGGTCAGCGAGGAACGGCGCTTCCGGGCGAGTCAGCACAAACTGAACGGTGTTGTCGTCCACCCTTTTC                                                                                          >  SRR4302543.440363/1‑94 (MQ=60)
     CAGAATAGAGGCGAAGTCCATTGCCAGGTCAGCGAGGAACGGCGCTTCCGGGCGAGTCAGCACAAACTGAACGGTGTTGTCGTCCACCCTTTTC                                                                                          >  SRR4302543.4641728/1‑94 (MQ=60)
     CAGAATAGAGGCGAAGTCCATTGCCAGGTCAGCGAGGAACGGCGCTTCCGGGCGAGTCAGCACAAACTGAACGGTGTTGTCGTCCACCCTTTTC                                                                                          >  SRR4302543.3894491/1‑94 (MQ=60)
     CAGAATAGAGGCGAAGTCCATTGCCAGGTCAGCGAGGAACGGCGCTTCCGGGCGAGTCAGCACAAACTGAACGGTGTTGTCGTCCACCCTTTTC                                                                                          >  SRR4302543.7952416/1‑94 (MQ=60)
     CAGAATAGAGGCGAAGTCCATTGCCAGGTCAGCGAGGAACGGCGCTTCCGGGCGAGTCAGCACAAACTGAACGGTGTTGTCGTCCACCCTTTTC                                                                                          >  SRR4302543.4394558/1‑94 (MQ=60)
      AGAATAGAGGCGAAGTCCATTGCCAGGTCAGCGAGGAACGGCGCTTCCGGGCGAGTCAGCACAAACTGAACGGTGTTGTCGTCCACCCTTTTCA                                                                                         >  SRR4302543.4435403/1‑94 (MQ=60)
      AGAATAGAGGCGAAGTCCATTGCCAGGTCAGCGAGGAACGGCGCTTCCGGGCGAGTCAGCACAAACTGAACGGTGTTGTCGTCCACCCTTTTCA                                                                                         <  SRR4302543.6182480/94‑1 (MQ=60)
      AGAATAGAGGCGAAGTCCATTGCCAGGTCAGCGAGGAACGGCGCTTCCGGGCGAGTCAGCACAAACTGAACGGTGTTGTCGTCCACCCTTTTCA                                                                                         >  SRR4302543.4977198/1‑94 (MQ=60)
        AATAGAGGGGAAGTCCATTGCCAGGTCAGCGAGGAACGGCGCTTCCGGGCGAGTCAGCACAAACTGAACGGTGTTGTCGTCCACCCTTTTCACT                                                                                       <  SRR4302543.1436233/94‑1 (MQ=60)
        AATAGAGGCGAAGTCCATTGCCAGGTCAGCGAGGAACGGCGCTTCCGGGCGAGTCAGCACAAACTGAACGGTGTTGTCGTCCACCCTTTTCACT                                                                                       >  SRR4302543.1451661/1‑94 (MQ=60)
        AATAGAGGCGAAGTCCATTGCCAGGTCAGCGAGGAACGGCGCTTCCGGGCGAGTCAGCACAAACTGAACGGTGTTGTCGTCCACCCTTTTCACT                                                                                       <  SRR4302543.316663/94‑1 (MQ=60)
        AATAGAGGCGAAGTCCATTGCCAGGTCAGCGAGGAACGGCGCTTCCGGGCGAGTCAGCACAAACTGAACGGTGTTGTCGTCCACCCTTTTCACT                                                                                       <  SRR4302543.7614160/94‑1 (MQ=60)
        AATAGAGGCGAAGTCCATTGCCAGGTCAGCGAGGAACGGCGCTTCCGGGCGAGTCAGCACAAACTGAACGGTGTTGTCGTCCACCCTTTTCACT                                                                                       >  SRR4302543.7665452/1‑94 (MQ=60)
        AATAGAGGCGAAGTCCATTGCCAGGTCAGCGAGGAACGGCGCTTCCGGGCGAGTCAGCACAAACTGAACGGTGTTGTCGTCCACCCTTTTCACT                                                                                       >  SRR4302543.4657835/1‑94 (MQ=60)
        AATAGAGGCGAAGTCCATTGCCAGGTCAGCGAGGAACGGCGCTTCCGGGCGAGTCAGCACAAACTGAACGGTGCTGTCGTCCACCCTTTTCACT                                                                                       >  SRR4302543.5547477/1‑94 (MQ=60)
         ATAGAGGCGAAGTCCATTGCCAGGTCAGCGAGGAACGGCGCTTCCGGGCGAGTCAGCACAAACTGAACGGTGTTGTCGTCCACCCTTTTCACTT                                                                                      >  SRR4302543.3813683/1‑94 (MQ=60)
           AGAGGCGAAGTCCATTGCCAGGTCAGCGAGGAACGGCGCTTCCGGGCGAGTCAGCACAAACTGAACGGTGTTGTCGTCCACCCTTTTCACTTCC                                                                                    >  SRR4302543.6478762/1‑94 (MQ=60)
           AGAGGCGAAGTCCATTGCCAGGTCAGCGAGGAACGGCGCTTCCGGGCGAGTCAGCACAAACTGAACGGTGTTGTCGTCCACCCTTTTCACTTCA                                                                                    >  SRR4302543.394583/1‑94 (MQ=60)
           AGAGGCGAAGTCCATTGCCAGGTCAGCGAGGAACGGCGCTTCCGGGCGAGTCAGCACAAACTGAACGGTGTTGTCGTCCACCCTTTTCACTTCA                                                                                    >  SRR4302543.2332955/1‑94 (MQ=60)
           AGAGGCGAAGTCCATTGCCAGGTCAGCGAGGAACGGCGCTTCCGGGCGAGTCAGCACAAACTGAACGGTGTTGTCGTCCACCCTTTTCACTTCA                                                                                    <  SRR4302543.3583812/94‑1 (MQ=60)
           AGAGGCGAAGTCCATTGCCAGGTCAGCGAGGAACGGCGCTTCCGGGCGAGTCAGCACAAACTGAACGGTGTTGTCGTCCACCCTTTTCACTTCA                                                                                    >  SRR4302543.428002/1‑94 (MQ=60)
           AGAGGCGAAGTCCATTGCCAGGTCAGCGAGGAACGGCGCTTCCGGGCGAGTCAGCACAAACTGAACGGTGTTGTCGTCCACCCTTTTCACTTCA                                                                                    <  SRR4302543.486086/94‑1 (MQ=60)
           AGAGGCGAAGTCCATTGCCAGGTCAGCGAGGAACGGCGCTTCCGGGCGAGTCAGCACAAACTGAACGGTGTTGTCGTCCACCCTTTTCACTTCA                                                                                    >  SRR4302543.4828262/1‑94 (MQ=60)
            GAGGCGAAGTCCATTGCCAGGTCAGCGAGGAACGGCGCTTCCGGGCGAGTCAGCACAAACTGAACGGTGTTGTCGTCCACCCTTTTCACTTCAC                                                                                   <  SRR4302543.2531131/94‑1 (MQ=60)
             AGGCGAAGTCCATTGCCAGGTCAGCGAGGAACGGCGCTTCCGGGCGAGTCAGCACAAACTGAACGGTGTTGTCGTCCACCCTTTTCACTTCACT                                                                                  >  SRR4302543.963200/1‑94 (MQ=60)
             AGGCGAAGTCCATTGCCAGGTCAGCGAGGAACGGCGCTTCCGGGCGAGTCAGCACAAACTGAACGGTGTTGTCGTCCACCCTTTTCACTTCACT                                                                                  >  SRR4302543.595194/1‑94 (MQ=60)
              GGCGAAGTCCATTGCCAGGTCAGCGAGGAACGGCGCTTCCGGGCGAGTCAGCACAAACTGAACGGTGTTGTCGTCCACCCTTTTCACTTCACTG                                                                                 <  SRR4302543.5162415/94‑1 (MQ=60)
               GCGAAGTCCATTGCCAGGTCAGCGAGGAACGGCGCTTCCGGGCGAGTCAGCACAAACTGAACGGTGTTGTCGTCCACCCTTTTCACTTCACTGA                                                                                <  SRR4302543.158399/94‑1 (MQ=60)
               GCGAAGTCCATTGCCAGGTCAGCGAGGAACGGCGCTTCCGGGCGAGTCAGCACAAACTGAACGGTGTTGTCGTCCACCCTTTTCACTTCACTGA                                                                                <  SRR4302543.373456/94‑1 (MQ=60)
                CGAAGTCCATTGCCAGGTCAGCGAGGAACGGCGCTTCCGGGCGAGTCAGCACAAACTGAACGGTGTTGTCGTCCACCCTTTTCACTTCACTGAT                                                                               >  SRR4302543.6829452/1‑94 (MQ=60)
                CGAAGTCCATTGCCAGGTCAGCGAGGAACGGCGCTTCCGGGCGAGTCAGCACAAACTGAACGGTGTTGTCGTCCACCCTTTTCACTTCACTGAT                                                                               <  SRR4302543.3211764/94‑1 (MQ=60)
                CGAAGTCCATTGCCAGGTCAGCGAGGAACGGCGCTTCCGGGCGAGTCAGCACAAACTGAACGGTGTTGTCGTCCACCCTTTTCACTTCACTGAT                                                                               <  SRR4302543.7688558/94‑1 (MQ=60)
                CGAAGTCCATTGCCAGGTCAGCGAGGAACGGCGCTTCCGGGCGAGTCAGCACAAACTGAACGGTGTTGTCGTCCACCCTTTTCACTTCACTGAC                                                                               >  SRR4302543.4041823/1‑94 (MQ=60)
                CGAAGTCCATTGCCAGGTCAGCGAGGAACGGCGATTCCGGGCGAGTCAGCACAAACTGAACGGTGTTGTCGTCCACCCTTTTCACTTCACTGAT                                                                               <  SRR4302543.7688557/94‑1 (MQ=60)
                 GAAGTCCATTGCCAGGTCAGCGAGGAACGGCGCTTCCGGGCGAGTCAGCACAAACTGAACGGTGTTGTCGTCCACCCTTTTCACTTCACTGATC                                                                              >  SRR4302543.6676203/1‑94 (MQ=60)
                 GAAGTCCATTGCCAGGTCAGCGAGGAACGGCGCTTCCGGGCGAGTCAGCACAAACTGAACGGTGTTGTCGTCCACCCTTTTCACTTCACTGATC                                                                              <  SRR4302543.5033831/94‑1 (MQ=60)
                 GAAGTCCATTGCCAGGTCAGCGAGGAACGGCGCTTCCGGGCGAGTCAGCACAAACTGAACGGCGATGTAGTCCACCCTTTTCACTTCACTGATC                                                                              >  SRR4302543.1640069/1‑94 (MQ=60)
                 GAAGTCCATTGCCAGGTCAGCGAGGAACAGCGCTTCCGGGCGAGTCAGCACAAACTGAACGGTGTTGTCGTCCACCCTTTTCACTTCACTGATC                                                                              >  SRR4302543.4168629/1‑94 (MQ=60)
                  AAGTCCATTGCCAGGTCAGCGAGGAACGGCGCTTCCGGGCGAGTCAGCACAAACTGAACGGTGTTGTCGTCCACCCTTTTCACTTCACTGATCA                                                                             >  SRR4302543.808446/1‑94 (MQ=60)
                  AAGTCCATTGCCAGGTCAGCGAGGAACGGCGCTTCCGGGCGAGTCAGCACAAACTGAACGGTGTTGTCGTCCACCCTTTTCACTTCACTGATCA                                                                             <  SRR4302543.7307956/94‑1 (MQ=60)
                  AAGTCCATTGCCAGGTCAGCGAGGAACGGCGCTTCCGGGCGAGTCAGCACAAACTGAACGGTGTTGTCGTCCACCCTTTTCACTTCACTGATCA                                                                             <  SRR4302543.1991251/94‑1 (MQ=60)
                   AGTCCATTGCCAGGTCAGCGAGGAACGGCGCTTCCGGGCGAGTCAGCACAAACTGAACGGTGTTGTCGTCCACCCTTTTCACTTCACTGATCAG                                                                            >  SRR4302543.6480776/1‑94 (MQ=60)
                   AGTCCATTGCCAGGTCAGCGAGGAACGGCGCTTCCGGGCGAGTCAGCACAAACTGAACGGTGTTGTCGTCCACCCTTTTCACTTCACTGATCAG                                                                            >  SRR4302543.6480536/1‑94 (MQ=60)
                     TCCATTGCCAGGTCAGCGAGGAACGGCGCTTCCGGGCGAGTCAGCACAAACTGAACGGTGTTGTCGTCCACCCTTTTCACTTCACTGATCAGCT                                                                          >  SRR4302543.6594340/1‑94 (MQ=60)
                     TCCATTGCCAGGTCAGCGAGGAACGGCGCTTCCGGGCGAGTCAGCACAAACTGAACGGTGTTGTCGTCCACCCTTTTCACTTCACTGATCAGCT                                                                          <  SRR4302543.418416/94‑1 (MQ=60)
                      CCATTGCCAGGTCAGCGAGGAACGGCGCTTCCGGGCGAGTCAGCACAAACTGAACGGTGTTGTCGTCCACCCTTTTCACTTCACTGATCAGCTC                                                                         <  SRR4302543.1928403/94‑1 (MQ=60)
                      CCATTGCCAGGTCAGCGAGGAACGGCGCTTCCGGGCGAGTCAGCACAAACTGAACGGTGTTGTCGTCCACCCTTTTCACTTCACTGATCAGCTC                                                                         <  SRR4302543.1928404/94‑1 (MQ=60)
                       CATTGCCAGGTCAGCGAGGAACGGCGCTTCCGGGCGAGTCAGCACAAACTGAACGGTGTTGTCGTCCACCCTTTTCACTTCACTGATCAGCTCT                                                                        <  SRR4302543.5784733/94‑1 (MQ=60)
                       CATTGCCAGGTCAGCGAGGAACGGCGCTTCCGGGCGAGTCAGCACAAACTGAACGGTGTTGTCGTCCACCCTTTTCACTTCACTGATCAGCTCT                                                                        <  SRR4302543.4189059/94‑1 (MQ=60)
                       CATTGCCAGGTCAGCGAGGAACGGCGCTTCCGGGCGAGTCAGCACAAACTGAACGGTGTTGTCGTCCACCCTTTTCACTTCACTGATCAGCTCT                                                                        <  SRR4302543.3301212/94‑1 (MQ=60)
                       CATTGCCAGGTCAGCGAGGAACGGCGCTTCCGGGCGAGTCAGCACAAACTGAACGGTGTTGTCGTCCACCCTTTTCACTTCACTGATCAGCTCT                                                                        >  SRR4302543.4049714/1‑94 (MQ=60)
                       CATTGCCAGGTCAGCGAGGAACGGCGCTTCCGGGCGAGTCAGCACAAACTGAACGGTGTTGTCGTCCACCCTTTTCACTTCACTGATCAGCTCT                                                                        >  SRR4302543.6145845/1‑94 (MQ=60)
                        ATTGCCAGGTCAGCGAGGAACGGCGCTTCCGGGCGAGTCAGCACAAACTGAACGGTGTTGTCGTCCACCCTTTTCACTTCACTGATCAGCTCTG                                                                       >  SRR4302543.3215848/1‑94 (MQ=60)
                        ATTGCCAGGTCAGCGAGGAACGGCGCTTCCGGGCGAGTCAGCACAAACTGAACGGTGTTGTCGTCCACCCTTTTCACTTCACTGATCAGCTCTG                                                                       >  SRR4302543.1301940/1‑94 (MQ=60)
                        ATTGCCAGGTCAGCGAGGAACGGCGCTTCCGGGCGAGTCAGCACAAACTGAACGGTGTTGTCGTCCACCCTTTTCACTTCACTGATCAGCTCTG                                                                       <  SRR4302543.2066186/94‑1 (MQ=60)
                        ATTGCCAGGTCAGCGAGGAACGGCGCTTCCGGGCGAGTCAGCACAAACTGAACGGTGTTGTCGTCCACCCTTTTCACTTCACTGATCAGCTCTG                                                                       >  SRR4302543.1649565/1‑94 (MQ=60)
                        ATTGCCAGGTCAGCGAGGAACGGCGCTTCCGGGCGAGTCAGCACAAACTGAACGGTGTTGTCGTCCACCCTTTTCACTTCACTGATCAGCTCTG                                                                       >  SRR4302543.2063839/1‑94 (MQ=60)
                        ATTGCCAGGTCAGCGAGGAACGGCGCTTCCGGGCGAGTCAGCACAAACTGAACGGGGTTGTCGTCCACCCTTTTCACTTCACTGATCAGCTCTG                                                                       >  SRR4302543.7807618/1‑94 (MQ=60)
                          TGCCAGGTCAGCGAGGAACGGCGCTTCCGGGCGAGTCAGCACAAACTGAACGGTGTTGTCGTCCACCCTTTTCACTTCACTGATCAGCTCTGGC                                                                     >  SRR4302543.7003006/1‑94 (MQ=60)
                          TGCCAGGTCAGCGAGGAACGGCGCTTCCGGGCGAGTCAGCACAAACTGAACGGTGTTGTCGTCCACCCTTTTCACTTCACTGATCAGCTCTGGC                                                                     <  SRR4302543.115603/94‑1 (MQ=60)
                           GCCAGGTCAGCGAGGAACGGCGCTTCCGGGCGAGTCAGCACAAACTGAACGGTGTTGTCGTCCACCCTTTTCACTTCACTGATCAGCTCTGGCA                                                                    <  SRR4302543.7725368/94‑1 (MQ=60)
                           GCCAGGTCAGCGAGGAACGGCGCTTCCGGGCGAGTCAGCACAAACTGAACGGTGTTGTCGTCCACCCTTTTCACTTCACTGATCAGCTCTGGCA                                                                    <  SRR4302543.7569758/94‑1 (MQ=60)
                            CCAGGTCAGCGAGGAACGGCGCTTCCGGGCGAGTCAGCACAAACTGAACGGTGTTGTCGTCCACCCTTTTCACTTCACTGATCAGCTCTGGCAA                                                                   >  SRR4302543.6674612/1‑94 (MQ=60)
                            CCAGGTCAGCGAGGAACGGCGCTTCCGGGCGAGTCAGCACAAACTGAACGGCGTTGTCGTCCACCCTTTTCCCTTCACTGATCAGCTCTcgcag                                                                   >  SRR4302543.6674613/1‑89 (MQ=60)
                             CAGGTCAGCGAGGAACGGCGCTTCCGGGCGAGTCAGCACAAACTGAACGGTGTTGTCGTCCACCCTTTTCACTTCACTGATCAGCTCTGGCAAG                                                                  <  SRR4302543.201579/94‑1 (MQ=60)
                             CAGGTCAGCGAGGAACGGCGCTTCCGGGCGAGTCAGCACAAACTGAACGGTGTTGTCGTCCACCCTTTTCACTTCACTGATCAGCTCTGGCAAG                                                                  >  SRR4302543.3443984/1‑94 (MQ=60)
                             CAGGTCAGCGAGGAACGGCGCTTCCGGGCGAGTCAGCACAAACTGAACGGTGTTGTCGTCCACCCTTTTCACTTCACTGATCAGCTCTGGCAAG                                                                  <  SRR4302543.1955333/94‑1 (MQ=60)
                              AGGTCAGCGAGGAACGGCGCTTCCGGGCGAGTCAGCACAAACTGAACGGTGTTGTCGTCCACCCTTTTCACTTCACTGATCAGCTCTGGCAAGC                                                                 >  SRR4302543.3024307/1‑94 (MQ=60)
                               GGTCAGCGAGGAACGGCGCTTCCGGGCGAGTCAGCACAAACTGAACGGTGTTGTCGTCCACCCTTTTCACTTCACTGATCAGCTCTGGCAAGCC                                                                >  SRR4302543.1571805/1‑94 (MQ=60)
                                GTCAGCGAGGAACGGCGCTTCCGGGCGAGTCAGCACAAACTGAACGGTGTTGTCGTCCACCCTTTTCACTTCACTGATCAGCTCTGGAAAGCCC                                                               >  SRR4302543.341506/1‑94 (MQ=60)
                                 TCAGCGAGTAAAGGCGCTGCCGGGCCAGTCAGCATAAACGTAACGGTGTTGTCGTCCACCCTTTTCACTTCACTGATCAGCTCTGGCAAGCCCA                                                              <  SRR4302543.4886592/94‑1 (MQ=60)
                                  CAGCGAGGAACGGCGCTTCCGGGCGAGTCAGCACAAACTGAACGGTGTTGTCGTCCACCCTTTTCACTTCACTGATCAGCTCTGGCAAGCCCAT                                                             <  SRR4302543.4425934/94‑1 (MQ=60)
                                  CAGCGAGGAACGGCGCTTCCGGGCGAGTCAGCACAAACTGAACGGTGTTGTCGTCCACCCTTTTCACTTCACTGATCAGCTCTGGCAAGCCCAT                                                             <  SRR4302543.3408702/94‑1 (MQ=60)
                                  CAGCGAGGAACGGCGCTTCCGGGCGAGTCAGCACAAACTGAACGGTGTTGTCGTCCACCCTTTTCACTTCACTGATCAGCTCTGGCAAGCCCAT                                                             >  SRR4302543.3539746/1‑94 (MQ=60)
                                  CAGCGAGGAACGGCGCTTCCGGGCGAGTCAGCACAAACTGAACGGTGTTGTCGTCCACCCTTTTCACTTCACTGATCAGCTCTGGCAAGCCCAT                                                             <  SRR4302543.2271276/94‑1 (MQ=60)
                                   AGCGAGGAACGGCGCTTCCGGGCGAGTCAGCACAAACTGAACGGTGTTGTCGTCCACCCTTTTCACTTCACTGATCAGCTCTGGCAAGCCCATG                                                            <  SRR4302543.5325706/94‑1 (MQ=60)
                                    GCGAGGAACGGCGCTTCCGGGCGAGTCAGCACAAACTGAACGGTGTTGTCGTCCACCCTTTTCACTTCACTGATCAGCTCTGGCAAGCCCATGC                                                           <  SRR4302543.1882635/94‑1 (MQ=60)
                                    GCGAGGAACGGCGCTTCCGGGCGAGTCAGCACAAACTGAACGGTGTTGTCGTCCACCCTTTTCACTTCACTGATCAGCTCTGGCAAGCCCATGC                                                           <  SRR4302543.2867542/94‑1 (MQ=60)
                                    GCGAGGAACGGCGCTTCCGGGCGAGTCAGCACAAACTGAACGGTGTTGTCGTCCACCCTTTTCACTTCACTGATCAGCTCTGGCAAGCCCATGC                                                           <  SRR4302543.1580350/94‑1 (MQ=60)
                                     CGAGGAACGGCGCTTCCGGGCGAGTCAGCACAAACTGAACGGTGTTGTCGTCCACCCTTTTCACTTCACTGATCAGCTCTGGCAAGCCCATGCC                                                          >  SRR4302543.1527951/1‑94 (MQ=60)
                                      GAGGAACGGCGCTTCCGGGCGAGTCAGCACAAACTGAACGGTGTTGTCGTCCACCCTTTTCACTTCACTGATCAGCTCTGGCAAGCCCATGCCT                                                         >  SRR4302543.4731723/1‑94 (MQ=60)
                                      GAGGAACGGCGCTTCCGGGCGAGTCAGCACAAACTGAACGGTGTTGTCGTCCACCCTTTTCACTTCACTGATCAGCTCTGGCAAGCCCATGCCT                                                         >  SRR4302543.4152442/1‑94 (MQ=60)
                                      GAGGAACGGCGCTTCCGGGCGAGTCAGCACAAACTGAACGGTGTTGTCGTCCACCCTTTTCACTTCACTGATCAGCTCTGGCAAGCCCATGCCT                                                         >  SRR4302543.6827167/1‑94 (MQ=60)
                                       AGGAACGGCGCTTCCGGGCGAGTCAGCACAAACTGAACGGTGTTGTCGTCCACCCTTTTCACTTCACTGATCAGCTCTGGCAAGCCCATGCCTT                                                        >  SRR4302543.2474121/1‑94 (MQ=60)
                                       AGGAACGGCGCTTCCGGGCGAGTCAGCACAAACTGAACGGTGTTGTCGTCCACCCTTTTCACTTCACTGATCAGCTCTGGCAAGCCCATGCCTT                                                        >  SRR4302543.5109333/1‑94 (MQ=60)
                                        GGAACGGCGCTTCCGGGCGAGTCAGCACAAACTGAACGGTGTTGTCGTCCACCCTTTTCACTTCACTGATCGNCTCTGGCAAGCCCATGCCTTC                                                       >  SRR4302543.5956927/1‑94 (MQ=60)
                                         GAACGGCGCTTCCGGGCGAGTCAGCACAAACTGAACGGTGTTGTCGTCCACCCTTTTCACTTCACTGATCAGCTCTGGCAAGCCCATGCCTTCG                                                      >  SRR4302543.5619126/1‑94 (MQ=60)
                                         GAACGGCGCTTCCGGGCGAGTCAGCACAAACTGAACGGTGTTGTCGTCCACCCTTTTCACTTCACTGATCAGCTCTGGCAAGCCCATGCCTTCG                                                      <  SRR4302543.7317384/94‑1 (MQ=60)
                                         GAACGGCGCTTCCGGGCGAGTCAGCACAAACTGAACGGTGTTGTCGTCCACCCTTTTCACTTCACTGATCAGCTCTGGCAAGCCCATGCCTTCG                                                      <  SRR4302543.5263873/94‑1 (MQ=60)
                                         GAACGGCGCTTCCGGGCGAGTCAGCACAAACTGAACGGTGTTGTCGTCCACCCTTTTCACTTCACTGATCAGCTCTGGCAAGCCCATGCCTTCG                                                      <  SRR4302543.5263872/94‑1 (MQ=60)
                                          AACGGCGCTTCCGGGCGAGTCAGCACAAACTGAACGGTGTTGTCGTCCACCCTTTTCACTTCACTGATCAGCTCTGGCAAGCCCATGCCTTCGA                                                     >  SRR4302543.1369435/1‑94 (MQ=60)
                                            CGGCGCTTCCTGGCGAGTCAGCACAAACTGAACGGTGTTGTCGTCCACCCTTTTCACTTCACTGATCAGCTCTGGCAAGCCCATGCCTTCGAAG                                                   <  SRR4302543.2122030/94‑1 (MQ=60)
                                            CGGCGCTTCCGGGCGAGTCAGCACAAACTGAACGGTGTTGTCGTCCACCCTTTTCACTTCACTTATCAGCTCTGGCAAGCCCATGCCTTCGAAG                                                   <  SRR4302543.6754810/94‑1 (MQ=60)
                                            CGGCGCTTCCGGGCGAGTCAGCACAAACTGAACGGTGTTGTCGTCCACCCTTTTCACTTCACTGATCAGCTCTGGCAAGCCCATGCCTTCGAAG                                                   <  SRR4302543.2744157/94‑1 (MQ=60)
                                            CGGCGCTTCCGGGCGAGTCAGCACAAACTGAACGGTGTTGTCGTCCACCCTTTTCACTTCACTGATCAGCTCTGGCAAGCCCATGCCTTCGAAG                                                   <  SRR4302543.5089133/94‑1 (MQ=60)
                                             GGCGCTTCCGGGCGAGTCAGCACAAACTGAACGGTGTTGTCGTCCTCCCTTTTCACTTCACTGATCAGCTCTGGCAAGCCCATGCCTTCGAAGT                                                  <  SRR4302543.510385/94‑1 (MQ=60)
                                             GGCGCTTCCGGGCGAGTCAGCACAAACTGAACGGTGTTGTCGTCCACCCTTTTCACTTCACTGATCAGCTCTCGAAAGCCCATGCCTTCGAAGT                                                  >  SRR4302543.5269190/1‑94 (MQ=60)
                                             CGCGCTTCCGGGCGAGTCAGCACAAACTGAACGGTGTTGTCGTCCACCCTTTTCACTTCACTGATCAGCTCTGGCAAGCCCATGCCTTCGAAGT                                                  <  SRR4302543.7394708/94‑1 (MQ=60)
                                              GCGCTTCCGGGCGAGTCAGCACAAACTGAACGGTGTTGTCGTCCACCCTTTTCACTTCACTGATCAGCTCTGGCAAGCCCATGCCTTCGAAGTA                                                 <  SRR4302543.4965621/94‑1 (MQ=60)
                                               CGCTTCCGGGCGAGTCAGCACAAACTGAACGGTGTTGTCGTCCACCCTTTTCACTTCACTGATCAGCTCTGGCAAGCCCATGCCTTCGAAGTAT                                                <  SRR4302543.573851/94‑1 (MQ=60)
                                               CGCTTCCGGGCGAGTCAGCACAAACTGAACGGTGTTGTCGTCCACCCTTTTCACTTCACTGATCAGCTCTGGCAAGCCCATGCCTTCGAAGTAT                                                <  SRR4302543.7610683/94‑1 (MQ=60)
                                               CGCTTCCGGGCGAGTCAGCACAAACTGAACGGTGTTGTCGTCCACCCTTTTCACTTCACTGATCAGCTCTGGCAAGCCCATGCCTTCGAAGTAT                                                <  SRR4302543.4614875/94‑1 (MQ=60)
                                                GCTTCCGGGCGAGTCAGCACAAACTGAACGGTGTTGTCGTCCACCCTTTTCACTTCACTGATCAGCTCTGGCAAGCCCATGCCTTCGAAGTATT                                               >  SRR4302543.7882757/1‑94 (MQ=60)
                                                GCTTCCGGGCGAGTCAGCACAAACTGAACGGTGTTGTCGTCCACCCTTTTCACTTCACTGATCAGCTCTGGCAAGCCCATGCCTTCGAAGTATT                                               >  SRR4302543.496372/1‑94 (MQ=60)
                                                GCTTCCGGGCGAGTCAGCACAAACTGAACGGTGTTGTCGTCCACCCTTTTCACTTCACTGATCAGCTCTGGCAAGCCCATGCCTTCGAAGTATT                                               <  SRR4302543.4468208/94‑1 (MQ=60)
                                                 CTTCCGGGCGAGTCAGCACAAACTGAACGGTGTTGTCGTCCACCCTTTTCACTTCACTGATCAGCTCTGGCAAGCCCATGCCTTCGAAGTATTC                                              <  SRR4302543.108238/94‑1 (MQ=60)
                                                 CTTCCGGGCGAGTCAGCACAAACTGAACGGTGTTGTCGTCCACCCTTTTCACTTCACTGATCAGCTCTGGCAAGCCCATGCCTTCGAAGTATTC                                              <  SRR4302543.3455075/94‑1 (MQ=60)
                                                 CTTCCGGGCGAGTCAGCACAAACTGAACGGTGTTGTCGTCCACCCTTTTCACTTCACTGATCAGCTCTGGCAAGCCCATGCCTTCGAAGTATTC                                              <  SRR4302543.1175354/94‑1 (MQ=60)
                                                 CTTCCGGGCGAGTCAGCACAAACTGAACGGTGTTGTCGTCCACCCTTTTCACTTCACTGATCAGCTCTGGCAAGCCCATGCCTTCGAAGTATTC                                              >  SRR4302543.4823816/1‑94 (MQ=60)
                                                 CTTCCGGGCGAGTCAGCACAAACTGAACGGTGTTGTCGTCCACCCTTTTCACTTCACTGATCAGCTCTGGCAAGCCCATGCCTTCGAAGTATTC                                              >  SRR4302543.7790842/1‑94 (MQ=60)
                                                 CTTCCGGGCGAGTCAGCACAAACTGAACAGTGTTGTCGTCCACCCTTTTCACTTCACTGATCAGCTCTGGCAAGCCCATGCCTTCGAAGTATTC                                              <  SRR4302543.108239/94‑1 (MQ=60)
                                                  TTCCGGGCGAGTCAGCACAAACTGAACGGTGTTGTCGTCCACCCTTTTCACTTCACTGATCAGCTCTGGCAAGCCCATGCCTTCGAAGTATTCG                                             <  SRR4302543.7860916/94‑1 (MQ=60)
                                                  TTCCGGGCGAGTCAGCACAAACTGAACGGTGTTGTCGTCCACCCTTTTCACTTCACTGATCAGCTCTGGCAAGCCCATGCCTTCGAAGTATTCG                                             >  SRR4302543.3811900/1‑94 (MQ=60)
                                                   TCCGGGCGAGTCAGCACAAACTGAACGGTGTTGTCGTCCACCCTTTTCACTTCACTGATCAGCTCTGGCAAGCCCATGCCTTCGAAGTATTCGT                                            <  SRR4302543.780782/94‑1 (MQ=60)
                                                   TCCGGGCGAGTCAGCACAAACTGAACGGTGTTGTCGTCCACCCTTTTCACTTCACTGATCAGCTCTGGCAAGCCCATGCCTTCGAAGTATTCGT                                            <  SRR4302543.657905/94‑1 (MQ=60)
                                                   TCCGGGCGAGTCAGCACAAACTGAACGGTGTTGTCGTCCACCCTTTTCACTTCACTGATCAGCTCTGGCAAGCCCATGCCTTCGAAGTATTCGT                                            <  SRR4302543.4251409/94‑1 (MQ=60)
                                                    CCGGGCGAGTCAGCACAAACTGAACGGTGTTGTCGTCCACCCTTTTCACTTCACTGATCAGCTCTGGCAAGCCCATGCCTTCGAAGTATTCGTA                                           <  SRR4302543.3973394/94‑1 (MQ=60)
                                                     CGGGCGAGTCAGCACAAACTGAACGGTGTTGTCGTCCACCCTTTTCACTTCACTGATCAGCTCTGGCAAGCCCATGCCTTCGAAGTATTCGTAG                                          <  SRR4302543.1218177/94‑1 (MQ=60)
                                                     CGGGCGAGTCAGCACAAACTGAACGGTGTTGTCGTCCACCCTTTTCACTTCACTGATCAGCTCTGGCAAGCCCATGCCTTCGAAGTATTCGTAG                                          <  SRR4302543.6631751/94‑1 (MQ=60)
                                                      GGGCGAGTCAGCACAAACTGAACGGTGTTGTCGTCCACCCTTTTCACTTCACTGATCAGCTCTGGCAAGCCCATGCCTTCGAAGTATTCGTAGC                                         >  SRR4302543.7925749/1‑94 (MQ=60)
                                                         CGAGTCAGCACAAACTGAACGGTGTTGTCGTCCACCCTTTTCACTTCACTGATCAGCTCTGGCAAGCCCATGCCTTCGAAGTATTCGTAGCTGC                                      >  SRR4302543.1765056/1‑94 (MQ=60)
                                                         CGAGTCAGCACAAACTGAACGGTGTTGTCGTCCACCCTTTTCACTTCACTGATCAGCTCTGGCAAGCCCATGCCTTCGAAGTATTCGTAGCTGC                                      <  SRR4302543.1054651/94‑1 (MQ=60)
                                                         CGAGTCAGCACAAACTGAACGGTGTTGTCGTCCACCCTTTTCACTTCACTGATCAGCTCTGGCAAGCCCATGCCTTCGAAGTATTCGTAGCTGC                                      >  SRR4302543.4895415/1‑94 (MQ=60)
                                                          GAGTCAGCACAAACTGAACGGTGTTGTCGTCCACCCTTTTCACTTCACTGATCAGCTCTGGCAAGCCCATGCCTTCGAAGTATTCGTAGCTGCC                                     <  SRR4302543.4101730/94‑1 (MQ=60)
                                                          GAGTCAGCACAAACTGAACGGTGTTGTCGTCCACCCTTTTCACTTCACTGATCAGCTCTGGCAAGCCCATGCCTTCGAAGTATTCGTAGCTGCC                                     <  SRR4302543.4101731/94‑1 (MQ=60)
                                                          GAGTCAGCACAAACTGAACGGTGTTGTCGTCCACCCTTTTCACTTCACTGATCAGCTCTGGCAAGCCCATGCCTTCGAAGTATTCGTAGCTGCC                                     >  SRR4302543.531201/1‑94 (MQ=60)
                                                           AGTCAGCACAAACTGAACGGTGTTGTCGTCCACCCTTTTCACTTCACTGATCAGCTCTGGCAAGCCCATGCCTTCGAAGTATTCGTAGCTGCCG                                    >  SRR4302543.7839156/1‑94 (MQ=60)
                                                            GTCAGCACAAACTGAACGGTGTTGTCGTCCACCCTTTTCACTTCACTGATCAGCTCTGGCAAGCCCATGCCTTCGAAGTATTCGTAGCTGCCGC                                   >  SRR4302543.6737401/1‑94 (MQ=60)
                                                            GTCAGCACAAACTGAACGGTGTTGTCGTCCACCCTTTTCACTTCACTGATCAGCTCTGGAAAGCCCATGCCTTCGAAGTATTCGTAGCTGCCGC                                   >  SRR4302543.1229772/1‑94 (MQ=60)
                                                             TCAGCACAAACTGAACGGTGTTGTCGTCCACCCTTTTCACTTCACTGATCAGCTCTGGCAAGCCCATGCCTTCGAAGTATTCGTAGCTGCCGCC                                  >  SRR4302543.7679323/1‑94 (MQ=60)
                                                              CAGCACAAACTGAACGGTGTTGTCGTCCACCCTTTTCACTTCACTGATCAGCTCTGGCAAGCCCATGCCTTCGAAGTATTCGTAGCTGCCGCCA                                 <  SRR4302543.1679348/94‑1 (MQ=60)
                                                              CAGCACAAACTGAACGGTGTTGTCGTCCACCCTTTTCACTTCACTGATCAGCTCTGGCAAGCCCATGCCTTCGAAGTATTCGTAGCTGCCGCCA                                 <  SRR4302543.3852845/94‑1 (MQ=60)
                                                               AGCACAAACTGAACGGTGTTGTCGTCCACCCTTTTCACTTCACTGATCAGCTCTGGCAAGCCCATGCCTTCGAAGTATTCGTAGCTGCCGCCAG                                <  SRR4302543.5567342/94‑1 (MQ=60)
                                                                  ACAAACTGAACGGTGTTGTCGTCCACCCTTTTCACTTCACTGATCAGCTCTGGCAAGCCCATGCCTTCGAAGTATTCGTAGCTGCCTCCAGAAA                             >  SRR4302543.5686238/1‑94 (MQ=60)
                                                                   CAAACTGAACGGTGTTGTCGTCCACCCTTTTCACTTCACTGATCAGCTCTGGCAAGCCCATGCCTTCGAAGTATTCGTAGCTGCCGCCAGAAAC                            <  SRR4302543.3256089/94‑1 (MQ=60)
                                                                   CAAACTGAACGGTGTTGTCGTCCACCCTTTTCACTTCACTGATCAGCTCTGGCAAGCCCATGCCTTCGAAGTATTCGTAGCTGCCGCCAGAAAC                            <  SRR4302543.138391/94‑1 (MQ=60)
                                                                   CAAACTGAACGGTGTTGTCGTCCACCCTTTTCACTTCACTGATCAGCTCTGGCAAGCCCATGCCTTCGAAGTATTCGTAGCTGCCGCCAGAAAC                            <  SRR4302543.5940592/94‑1 (MQ=60)
                                                                    AAACTGAACGGTGTTGTCGTCCACCCTTTTCACTTCACTGATCAGCTCTGGCAAGCCCATGCCTTCGAAGTATTCGTAGCTGCCGCCGGAAACT                           >  SRR4302543.1425976/1‑94 (MQ=60)
                                                                    AAACTGAACGGTGTTGTCGTCCACCCTTTTCACTTCACTGATCAGCTCTGGCAAGCCCATGCCTTCGAAGTATTCGTAGCTGCCGCCAGAAACT                           <  SRR4302543.6276800/94‑1 (MQ=60)
                                                                    AAACTGAACGGTGTTGTCGTCCACCCTTTTCACTTCACTGATCAGCTCTGGCAAGCCCATGCCTTCGAAGTATTCGTAGCTGCCGCCAGAAACT                           <  SRR4302543.2239507/94‑1 (MQ=60)
                                                                    AAACTGAACGGTGTTGTCGTCCACCCTTTTCACTTCACTGATCAGCTCTGGCAAGCCCATGCCTTCGAAGTATTCGTAGCTGCCGCCAGAAACT                           >  SRR4302543.2152601/1‑94 (MQ=60)
                                                                     AACTGAACGGTGTTGTCGTCCACCCTTTTCACTTCACTGATCAGCTCTGGCAAGCCCATGCCTTCGAAGTATTCGTAGCTGCCGCCAGAAACTT                          >  SRR4302543.6908209/1‑94 (MQ=60)
                                                                     AACTGAACGGTGTTGTCGTCCACCCTTTTCACTTCACTGATCAGCTCTGGCAAGCCCATGCCTTCGAAGTATTCGTAGCTGCCGCCAGAAACTT                          <  SRR4302543.6720802/94‑1 (MQ=60)
                                                                     AACTGAACGGTGTTGTCGTCCACCCTTTTCACTTCACTGATCAGCTCTGGCAAGCCCATGCCTTCGAAGTATTCGTAGCTGCCGCCAGAAACTT                          <  SRR4302543.3742746/94‑1 (MQ=60)
                                                                      ACTGAACGGTGTTGTCGTCCACCCTTTTCACTTCACTGATCAGCTCTGGCAAGCCCATGCCTTCGAAGTATTCGTAGCTGCCGCCAGAAACTTT                         <  SRR4302543.2430557/94‑1 (MQ=60)
                                                                       CTGAACGGTGTTGTCGTCCACCCTTTTCACTTCACTGATCAGCTCTGGCAAGCCCATGCCTTCGAAGTATTCGTAGCTGCCGCCAGAAACTTTA                        >  SRR4302543.2703737/1‑94 (MQ=60)
                                                                       CTGAACGGTGTTGTCGTCCACCCTTTTCACTTCACTGATCAGCTCTGGCAAGCCCATGCCTTCGAAGTATTCGTAGCTGCCGCCAGAAACTTTA                        >  SRR4302543.149612/1‑94 (MQ=60)
                                                                        TGAACGGTGTTGTCGTCCACCCTTTTCACTTCACTGATCAGCTCTGGCAAGCCCATGCCTTCGAAGTATTCGTAGCTGCCGCCAGAAACTTTAT                       <  SRR4302543.6555787/94‑1 (MQ=60)
                                                                        TGAACGGTGTTGTCGTCCACCCTTTTCACTTCACTGATCAGCTCTGGCAAGCCCATGCCTTCGAAGTATTCGTAGCTGCCGCCAGAAACTTTAT                       >  SRR4302543.3409191/1‑94 (MQ=60)
                                                                        TGAACGGTGTTGTCGTCCACCCTTTTCACTTCACTGATCAGCTCTGGCAAGCCCATGCCTTCGAAGTATTCGTAGCTGCCGCCAGAAACTTTAT                       <  SRR4302543.4145351/94‑1 (MQ=60)
                                                                         GAACGGTGTTGTCGTCCACCCTTTTCACTTCACTGATCAGCTCTGGCAAGCCCATGCCTTCGAAGTATTCGTAGCTGCCGCCAGAAACTTTATG                      >  SRR4302543.6244293/1‑94 (MQ=60)
                                                                         GAACGGTGTTGTCGTCCACCCTTTTCACTTCACTGATCAGCTCTGGCAAGCCCATGCCTTCGAAGTATTCGTAGCTGCCGCCAGAAACTTTATG                      <  SRR4302543.3271130/94‑1 (MQ=60)
                                                                          AACGGTGTTGTCGTCCACCCTTTTCACTTCACTGATCAGCTCTGGCAAGCCCATGCCTTCGAAGTATTCGTAGCTGCCGCCAGAAACTTTATGG                     >  SRR4302543.6057762/1‑94 (MQ=60)
                                                                          AACGGTGTTGTCGTCCACCCTTTTCACTTCACTGATCAGCTCTGGCAAGCCCATGCCTTCGAAGTATTCGTAGCTGCCGCCAGAAACTTTATGG                     <  SRR4302543.2105002/94‑1 (MQ=60)
                                                                          AACGGTGTTGTCGTCCACCCTTTTCACTTCACTGATCAGCTCTGGCAAGCCCATGCCTTCGAAGTATTCGTAGCTGCCGCCAGAAACTTTATGG                     <  SRR4302543.3574981/94‑1 (MQ=60)
                                                                          AACGGTGTTGTCGTCCACCCTTTTCACTTCACTGATCAGCTCTGGCAAGCCCATGCCTTCGAAGTATTCGTAGCTGCCGCCAGAAACTTTATGG                     <  SRR4302543.2574601/94‑1 (MQ=60)
                                                                            CGGTGTTGTCGTCCACCCTTTTCACTTCACTGATCAGCTCTGGCAAGCCCATGCCTTCGAAGTATTCGTAGCTGCCGCCAGAAACTTTATGGTA                   <  SRR4302543.6826445/94‑1 (MQ=60)
                                                                            CGGTGTTGTCGTCCACCCTTTTCACTTCACTGATCAGCTCTGGCAAGCCCATGCCTTCGAAGTATTCGTAGCTGCCGCCAGAAACTTTATGGTA                   >  SRR4302543.2767343/1‑94 (MQ=60)
                                                                             GGTGTTGTCGTCCACCCTTTTCACTTCACTGATCAGCTCTGGCAAGCCCATGCCTTCGAAGTATTCGTAGCTGCCGCCAGAAACTTTATGGTAC                  >  SRR4302543.1984647/1‑94 (MQ=60)
                                                                             GGTGTTGTCGTCCACCCTTTTCACTTCACTGATCAGCTCTGGCAAGCCCATGCCTTCGAAGTATTCGTAGCTGCCGCCAGAAACTTTATGGTAC                  <  SRR4302543.3071505/94‑1 (MQ=60)
                                                                             GGTGTTGTCGTCCACCCTTTTCACTTCACTGATCAGCTCTGGCAAGCCCATGCCTTCGAAGTATTCGTAGCTGCCGCCAGAAACTTTATGGTAC                  <  SRR4302543.5667521/94‑1 (MQ=60)
                                                                              GTGTTGTCGTCCACCCTTTTCACTTCACTGATCAGCTCTGGCAAGCCCATGCCTTCGAAGTATTCGTAGCTGCCGCCAGAAACTTTATGGTACG                 <  SRR4302543.3605807/94‑1 (MQ=60)
                                                                              GTGTTGTCGTCCACCCTTTTCACTTCACTGATCAGCTCTGGCAAGCCCATGCCTTCGAAGTATTCGTAGCTGCCGCCAGAAACTTTATGGTACG                 >  SRR4302543.7904143/1‑94 (MQ=60)
                                                                              GTGTTGTCGTCCACCCTTTTCACTTCACTGATCAGCTCTGGCAAGCCCATGCCTTCGAAGTATTCGTAGCTGCCGCCAGAAACTTTATGGTACG                 <  SRR4302543.3130055/94‑1 (MQ=60)
                                                                              GTGTGGTCGTCCACCCTTTTCACTTCACTGATCAGCTCTGGCAAGCCCATGCCTTCGAAGTATTCGTAGCTGCCGCCAGAAACTTTATGGTACG                 <  SRR4302543.3605806/94‑1 (MQ=60)
                                                                               TGTTGTCGTCCACCCTTTTCACTTCACTGATCAGCTCTGGCAAGCCCATGCCTTCGAAGTATTCGTAGCTGCCGCCAGAAACTTTATGGTACGG                >  SRR4302543.4370842/1‑94 (MQ=60)
                                                                               TGTTGTCGTCCACCCTTTTCACTTCACTGATCAGCTCTGGCAAGCCCATGCCTTCGAAGTATTCGTAGCTGCCGCCAGAAACTTTATGGTACGG                <  SRR4302543.1440810/94‑1 (MQ=60)
                                                                                GTTGTCGTCCACCCTTTTCACTTCACTGATCAGCTCTGGCAAGCCCATGCCTTCGAAGTATTCGTAGCTGCCGCCAGAAACTTTATGGTACGGG               <  SRR4302543.3954482/94‑1 (MQ=60)
                                                                                GTTGTCGTCCACCCTTTTCACTTCACTGATCAGCTCTGGCAAGCCCATGCCTTCGAAGTATTCGTAGCTGCCGCCAGAAACTTTATGGTACGGG               >  SRR4302543.307128/1‑94 (MQ=60)
                                                                                GTTGTCGTCCACCCTTTTCACTTCACTGATCAGCTCTGGCAAGCCCATGCCTTCGAAGTATTCGTAGCTGCCGCCAGAAACTTTATGGTACGGG               <  SRR4302543.5736643/94‑1 (MQ=60)
                                                                                 TTGTCGTCCACCCTTTTCACTTCACTGATCAGCTCTGGCAAGCCCATGCCTTCGAAGTATTCGTAGCTGCCGCCAGAAACTTTATGGTACGGGT              <  SRR4302543.5670791/94‑1 (MQ=60)
                                                                                  TGTCGTCCCCCCTTTTCACTTCACTGATCAGCTCTGGCAAGCCCATGCCTTCGAAGTATTCGTAGCTGCCGCCAGAAACTTTATGGTACGGGTT             <  SRR4302543.6983783/94‑1 (MQ=60)
                                                                                  TGTCGTCCACCCTTTTCACTTCACTGATCAGCTCTGGCAAGCCCATGCCTTCGAAGTATTCGTAGCTGCCGCCAGAAACTTTATGGTACGGGTT             <  SRR4302543.6816654/94‑1 (MQ=60)
                                                                                  TGTCGTCCACCCTTTTCACTTCACTGATCAGCTCTGGCAAGCCCATGCCTTCGAAGTATTCGTAGCTGCCGCCAGAAACTTTATGGTACGGGTT             <  SRR4302543.6983782/94‑1 (MQ=60)
                                                                                  TGTCGTCCACCCTTTTCACTTCACTGATCAGCTCTGGCAAGCCCATGCCTTCGAAGTATTCGTAGCTGCCGCCAGAAACTTTATGGTACGGGTT             <  SRR4302543.6990991/94‑1 (MQ=60)
                                                                                     CGTCCACCCTTTTCACTTCACTGATCAGCTCTGGCAAGCCCATGCCTTCGAAGTATTCGTAGCTGCCGCCAGAAACTTTATGGTACGGGTTTTG          >  SRR4302543.1897473/1‑94 (MQ=60)
                                                                                       TCCACCCTTTTCACTTCACTGATCAGCTCTGGCAAGCCCATGCCTTCGAAGTATTCGTAGCTGCCGCCAGAAACTTTATGGTACGGGTTTTGCG        <  SRR4302543.7528795/94‑1 (MQ=60)
                                                                                       TCCACCCTTTTCACTTCACTGATCAGCTCTGGCAAGCCCATGCCTTCGAAGTATTCGTAGCTGCCGCCAGAAACTTTATGGTACGGGTTTTGCG        <  SRR4302543.4214635/94‑1 (MQ=60)
                                                                                       TCCACCCTTTTCACTTCACTGATCAGCTCTGGCAAGCCCATGCCTTCGAAGTATTCGTAGCTGCCGCCAGAAACTTTATGGTACGGGTTTTGCG        >  SRR4302543.1410284/1‑94 (MQ=60)
                                                                                        CCACCCTTTTCACTTCACTGATCAGCTCTGGCAAGCCCATGCCTTCGCAGTATTCGTAGCTGCCGCCCGAAACTTTATGGTACGcggtgtgcgc       >  SRR4302543.6474908/1‑84 (MQ=60)
                                                                                        CCACCCTTTTCACTTCACTGATCAGCTCTGGCAAGCCCATGCCTTCGAAGTATTCGTAGCTGCCGCCGGAAACTTTATGGTACGGGTGTTGCGC       >  SRR4302543.6474907/1‑94 (MQ=60)
                                                                                        CCACCCTTTTCACTTCACTGATCAGCTCTGGCAAGCCCATGCCTTCGAAGTATTCGTAGCTGCCGCCAGAAACTTTATGGGACGGGTTTTGCGG       >  SRR4302543.2127543/1‑94 (MQ=60)
                                                                                         CACCCTTTTCACTTCACTGATCAGCTCTGGCAAGCCCATGCCTTCGAAGTATTCGTAGCTGCCGCCAGAAACTTTATGGTACGGGTTTTGCGCG      >  SRR4302543.7473166/1‑94 (MQ=60)
                                                                                          ACCCTTTTCACTTCACTGATCAGCTCTGGCAAGCCCATGCCTTCGAAGTATTCGTAGCTGCCGCCAGAAACTTTATGGTACGGGTTTTGCGCGT     >  SRR4302543.2536777/1‑94 (MQ=60)
                                                                                           CCCTTTTCACTTCACTGATCAGCTCTGGCAAGCCCATGCCTTCGAAGTATTCGTAGCTGCCGCCAGAAACTTTATGGTACGGGTTTTGCGCGTT    >  SRR4302543.7028775/1‑94 (MQ=60)
                                                                                           CCCTTTTCACTTCACTGATCAGCTCTGGCAAGCCCATGCCTTCGAAGTATTCGTAGCTGCCGCCAGAAACTTTATGGTACGGGTTTTGCGCGTT    >  SRR4302543.4517746/1‑94 (MQ=60)
                                                                                           CCCTTTTCACTTCACTGATCAGCTCTGGCAAGCCCATGCCTTCGAAGTATTCGTAGCTGCCGCCAGAAACTTTATGGTACGGGTTTTGCGCGTT    >  SRR4302543.63931/1‑94 (MQ=60)
                                                                                           CCCTTTTCACTTCACTGATCAGCTCTGGCAAGCCCATGCCTTCGAAGTATTCGTAGCTGCCGCCAGAAACTTTATGGTACGGGTTTTGCGCGTT    >  SRR4302543.1137888/1‑94 (MQ=60)
                                                                                            CCTTTTCACTTCACTGATCAGCTCTGGCAAGCCCATGCCTTCGAAGTATTCGTAGCTGCCGCCAGAAACTTTATGGTACGGGTTTTGCGCGTTT   <  SRR4302543.4691917/94‑1 (MQ=60)
                                                                                            CCTTTTCACTTCACTGATCAGCTCTGGCAAGCCCATGCCTTCGAAGTATTCGTAGCTGCCGCCAGAAACTTTATGGTACGGGTTTTGCGCGTTT   >  SRR4302543.1284331/1‑94 (MQ=60)
                                                                                             |                                                                                             
TTTGACAGAATAGAGGCGAAGTCCATTGCCAGGTCAGCGAGGAACGGCGCTTCCGGGCGAGTCAGCACAAACTGAACGGTGTTGTCGTCCACCTTTTTCACTTCACTGATCAGCTCTGGCAAGCCCATGCCTTCGAAGTATTCGTAGCTGCCGCCAGAAACTTTATGGTACGGGTTTTGCGCGTTTT  >  NC_000913/3704772‑3704958

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑