Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 1873 183.1 9376148 98.2% 9207377 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,792,026 A→G N138S (AAC→AGC)  rfaD → ADP‑L‑glycero‑D‑mannoheptose‑6‑epimerase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,792,0260AG99.6% 861.3 / NA 270N138S (AAC→AGC) rfaDADP‑L‑glycero‑D‑mannoheptose‑6‑epimerase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  major base G (121/148);  minor base T (0/1);  total (121/149)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 279 total aligned reads displayed.

AAATCCCGTTCCTGTACGCTTCTTCCGCAGCCACCTACGGCGGACGCACCTCCGACTTTATTGAATCCCGCGAGTACGAAAAACCGTTGAACGTCTACGGTTACTCAAAATTCCTGTTTGATGAATATGTTCGTCAAATCCTGCCTGAAGCGAACTCGCAGATTGTTGGCTTCCGTTATT  >  NC_000913/3791936‑3792115
                                                                                          |                                                                                         
aaaTCCCGTTCCTGTACGCTTCTTCCGCAGCCACCTACGGCGGACGCACCTCCGACTTTATTGAATCCCGCGAGTACGAAAAACCGTTGAGCg                                                                                         >  1:857523/1‑93 (MQ=255)
aaaTCCCGTTCCTGTACGCTTCTTCCGCAGCCACCTACGGCGGACGCACCTCCGACTTTATTGAATCCCGCGAGTACGAAAAACCGTTGAGCg                                                                                         >  2:3232107/1‑93 (MQ=255)
 aaTCCCGTTCCTGTACGCTTCTTCCGCAGCCACCTACGGCGGACGCACCTCCGACTTTATTGAATCCCGCGAGTACGAAAAACCGTTGAGCGt                                                                                        <  1:2526794/93‑1 (MQ=255)
 aaTCCCGTTCCTGTACGCTTCTTCCGCAGCCACCTACGGCGGACGCACCTCCGACTTTATTGAATCCCGCGAGTACGAAAAACCGTTGAGCGt                                                                                        <  2:4517027/93‑1 (MQ=255)
  aTCCCGTTCCTGTACGCTTCTTCCGCAGCCACCTACGGCGGACGCACCTCCGACTTTATTGAATCCCGCGAGTACGAAAAACCGTTGAGCGTc                                                                                       >  2:1404955/1‑93 (MQ=255)
  aTCCCGTTCCTGTACGCTTCTTCCGCAGCCACCTACGGCGGACGCACCTCCGACTTTATTGAATCCCGCGAGTACGAAAAACCGTTGAGCGTc                                                                                       >  1:2968074/1‑93 (MQ=255)
   tCCCGTTCCTGTACGCTTCTTCCGCAGCCACCTACGGCGGACGCACCTCCGACTTTATTGAATCCCGCGAGTACGAAAAACCGTTGAGCGTCt                                                                                      <  2:3607858/93‑1 (MQ=255)
     ccGTTCCTGTACGCTTCTTCCGCAGCCACCTACGGCGGACGCACCTCCGACTTTATTGAATCCCGCGAGTACGAAAAACCGTTGAGCGTCTAc                                                                                    >  2:332904/1‑93 (MQ=255)
     ccGTTCCTGTACGCTTCTTCCGCAGCCACCTACGGCGGACGCACCTCCGACTTTATTGAATCCCGCGAGTACGAAAAACCGTTGAGCGTCTAc                                                                                    >  2:3517535/1‑93 (MQ=255)
     ccGTTCCTGTACGCTTCTTCCGCAGCCACCTACGGCGGACGCACCTCCGACTTTATTGAATCACGCGAGTACGAAAAACCGTTGAGCGTCTAc                                                                                    >  1:2352278/1‑93 (MQ=255)
       gTTCCTGTACGCTTCTTCCGCAGCCACCTACGGCGGACGCACCTCCGACTTTATTGAATCCCGCGAGTACGAAAAACCGTTGAGCGGCTACgg                                                                                  >  1:185716/1‑93 (MQ=255)
       cttCCTGTACGCTTCTTCCGCAGCCACCTACGGCCGACGCTCCTCCGACTTTATTGAATCCCGCGAGTACGAAAAACCGTTGAGCGTCTACgg                                                                                  <  2:3734472/92‑1 (MQ=255)
        ttCCTGTACGCTTCTTCCGCAGCCACCTACGGCGGACGCACCTCCGACTTTATTGAATCCCGCGAGTACGAAAAACCGTTGAGCGTCTACGGt                                                                                 <  2:2333991/93‑1 (MQ=255)
        ttCCTGTACGCTTCTTCCGCAGCCACCTACGGCGGACGCACCTCCGACTTTATTGAATCCCGCGAGTACGAAAAACCGTTGAGCGTCTACGGt                                                                                 >  2:2146647/1‑93 (MQ=255)
        ttCCTGTACGCTTCTTCCGCAGCCACCTACGGCGGACGCACCTCCGACTTTATTGAATCCCGCGAGTACGAAAAACCGTTGAGCGTCTACGGt                                                                                 >  1:3948808/1‑93 (MQ=255)
        ttCCTGTACGCTTCTTCCGCAGCCACCTACGGCGGACGCACCTCCGACTTTATTGAATCCCGCGAGTACGAAAAACCGTTGAGCGTCTACGGt                                                                                 >  1:4067106/1‑93 (MQ=255)
        ttCCTGTACGCTTCTTCCGCAGCCACCTACGGCGGACGCACCTCCGACTTTATTGAATCCCGCGAGTACGAAAAACCGTTGAGCGTCTACGGt                                                                                 <  1:1069647/93‑1 (MQ=255)
         tCCTGTGCGCTTCTTCCGCAGCCACCTACGGCGGACGCACCTCCGACTTTATTGAATCCCGCGAGTACGAAAAACCGTTGAGCGTCTACGGtt                                                                                <  2:689344/93‑1 (MQ=255)
         tCCTGTACGCTTCTTCCGCAGCCACCTACGGCGGACGCACCTCCGACTTTATTGAATCCCGCGAGTACGAAAAACCGTTGAGCGTCTACGGtt                                                                                >  1:76564/1‑93 (MQ=255)
          ccTGTACGCTGCTTCCGCAGCCACCTACGGCGGACGCACCTCCGACTTTATTGAATCCCGCAAGTACGAAAACCCGTTGAGCGTCTTCGGTTa                                                                               <  2:1150172/93‑1 (MQ=255)
            tGTACGCTTCTTCCGCAGCCACCTACGGCGGACGCACCTCCGACTTTATTGAATCCCGCGAGTACGAAAAACCGTTGAGCGTCTACGGTTACt                                                                             >  1:3242151/1‑93 (MQ=255)
             gTACGCTTCTTCCGCAGCCACCTACGGCGGACGCTCCTCCGACTTTATTGAATCCCGCGAGTACGAAAAACCGTTGAGCGTCTACGGTTACTc                                                                            <  2:3212013/93‑1 (MQ=255)
                cGCTTCTTCCGCAGCCACCTACGGCGGACGCACCTCCGACTTTATTGAATCCCGCGAGTACGAAAAACCGTTGAGCGTCTACGGTTACTCaaa                                                                         >  1:4174106/1‑93 (MQ=255)
                 gCTTCTTCCGCAGCCACCTACGGCGGACGCACCTCCGACTTTATTGAATCCCGCGAGTACGAAAAACCGTTGAGCGTCTACGGTTACTCaaaa                                                                        <  1:2538976/93‑1 (MQ=255)
                 gCTTCTTCCGCAGCCACCTACGGCGGACGCACCTCCGACTTTATTGAATCCCGCGAGTACGAAAAACCGTTGAGCGTCTACGGTTACTCaaaa                                                                        <  1:1498471/93‑1 (MQ=255)
                 gCTTCTTCCGCAGCCACCTACGGCGGACGCACCTCCGACTTTATTGAATCCCGCGAGTACGAAAAACCGTTGAGCGTCTACGGTTACTCaaaa                                                                        <  2:2911689/93‑1 (MQ=255)
                   ttcttcCGCAGCCACCTACGGCGGACGCACCTCCGACTTTATTGAATCCCGCGAGTACGAAAAACCGTTGAGCGTCTACGGTTACTCAAAAtt                                                                      <  2:394877/93‑1 (MQ=255)
                       tcCGCAGCCACCTACGGCGGACGCACCTCCGACTTTATTGAATCCCGCGAGTACGAAAAACCGTTGAGCGTCTACGGTTACTCAAAATTCCTg                                                                  <  1:421892/93‑1 (MQ=255)
                         cGCAGCCACCTACGGCGGACGCACCTCCGACTTTATTGAATCCCGCGAGTACGAAAAACCGTTGAGCGTCTACGGTTACTCAAAATTCCTGtt                                                                <  1:479214/93‑1 (MQ=255)
                         cGCAGCCACCTACGGCGGACGCACCTCCGACTTTATTGAATCCCGCGAGTACGAAAAACCGTTGAGCGTCTACGGTTACTCAAAATTCCTGtt                                                                <  2:4605010/93‑1 (MQ=255)
                          gCAGCCACCTACGGCGGACGCACCTCCGACTTTATTGAATCCCGCGAGTACGAAAAACCGTTGAGCGTCTACGGTTACTCAAAATTCCTGttt                                                               >  1:3442041/1‑93 (MQ=255)
                          gCAGCCACCTACGGCGGACGCACCTCCGACTTTATTGAATCCCGCGAGTACGAAAAACCGTTGAGCGTCTACGGTTACTCAAAATTCCTGttt                                                               <  1:56478/93‑1 (MQ=255)
                          gCAGCCACCTACGGCGGACGCACCTCCGACTTTATTGAATCCCGCGAGTACGAAAAACCGTTGAGCGTCTACGGTTACTCAAAATTCCTGttt                                                               <  2:585548/93‑1 (MQ=255)
                            aGCCACCTACGGCGGACGCACCTCCGACTTTATTGAATCCCGCGAGTACGAAAAACCGTTGAGCGTCTACGGTTACTCAAAATTCCTGTTtga                                                             <  1:1240273/93‑1 (MQ=255)
                            aGCCACCTACGGCGGACGCACCTCCGACTTTATTGAATCCCGCGAGTACGAAAAACCGTTGAGCGTCTACGGTTACTCAAAATTCCTGTTtga                                                             >  2:399914/1‑93 (MQ=255)
                             gCCACCTACGGCGGACGCACCTCCGACTTTATTGAATCCCGCGAGTACGAAAAACCGTTGAGCGTCTACGGTTACTCAAAATTCCTGTTtgat                                                            >  1:3825623/1‑93 (MQ=255)
                             gCCACCTACGGCGGACGCACCTCCGACTTTATTGAATCCCGCGAGTACGAAAAACCGTTGAGCGTCTACGGTTACTCAAAATTCCTGTTtgat                                                            >  2:2408834/1‑93 (MQ=255)
                             gCCACCTACGGCGGACGCACCTCCGACTTTATTGAATCCCGCGAGTACGAAAAACCGTTGAGCGTCTACGGTTACTCAAAATTCCTGTTtgat                                                            >  1:2662745/1‑93 (MQ=255)
                             gCCACCTACGGCGGACGCACCTCCGACTTTATTGAATCCCGCGAGTACGAAAAAACGTTGAGCGTCTACGGTTGCTCAAAATTCGTGgtttgt                                                            >  2:1760609/1‑90 (MQ=255)
                               cACCTACGGCGGACGCACCTCCGACTTTATTGAATCCCGCGAGTACGAAAAACCGTTGAGCGTCTACGGTTACTCAAAATTCCTGTTtgatga                                                          <  2:1450354/93‑1 (MQ=255)
                                aCCTACGGCGGACGCACCTCCGACTTTATTGAATCCCGCGAGTACGAAAAACCGTTGAGCGTCTACGGTTACTCAAAATTCCTGTTTGATGaa                                                         <  2:1210225/93‑1 (MQ=255)
                                aCCTACGGCGGACGCACCTCCGACTTTATTGAATCCCGCGAGTACGAAAAACCGTTGAGCGTCTACGGTTACTCAAAATTCCTGTTTGATGaa                                                         <  2:2856251/93‑1 (MQ=255)
                                 ccTACGGCGGACGCACCTCCGACTTTATTGAATCCCGCGAGTACGAAAAACCGTTGAGCGTCTACGGTTACTCAAAATTCCTGTTTGATGAat                                                        >  2:1101275/1‑93 (MQ=255)
                                  cTACGGCGGACGCACCTCCGACTTTATTGAATCCCGCGAGTATGAAAAACCGTTGAGCGTCTACGGTTACTCAAAATTCCTGTTTGATGAata                                                       <  1:1753767/93‑1 (MQ=255)
                                  cTACGGCGGACGCACCTCCGACTTTATTGAATCCCGCGAGTACGAAAAACCGTTGAGCGTCTACGGTTACTCAAAATTCCTGTTTGATGAata                                                       >  2:2621052/1‑93 (MQ=255)
                                   tACGGCGGACGCACCTCCGACTTTATTGAATCCCGCGAGTACGAAAAACCGTTGAGCGTCTACGGTTACTCAAAATTCCTGTTTGATGAatat                                                      >  2:3267870/1‑93 (MQ=255)
                                   tACGGCGGACGCACCTCCGACTTTATTGAATCCCGCGAGTACGAAAAACCGTTGAGCGTCTACGGTTACTCAAAATTCCTGTTTGATGAatat                                                      <  2:2219999/93‑1 (MQ=255)
                                   tACGGCGGACGCACCTCCGACTTTATTGAATCCCGCGAGTACGAAAAACCGTTGAGCGTCTACGGTTACTCAAAATTCCTGTTTGATGAatat                                                      <  2:799415/93‑1 (MQ=255)
                                      ggcggACGCACCTCCGACTTTATTGAATCCCGCGAGTACGAAAAACCGTTGAGCGTCTACGGTTACTCAAAATTCCTGTTTGATGAATATGtt                                                   <  2:847284/93‑1 (MQ=255)
                                       gcggACGCACCTCCGACTTTATTGAATCCCGCGAGTACGAAAAACCGTTGAGCGTCTACGGTTACTCAAAATTCCTGTTTGATGAATATGTTc                                                  <  1:2797549/93‑1 (MQ=255)
                                       gcggACGCACCTCCGACTTTATTGAATCCCGCGAGTACGAAAAACCGTTGAGCGTCTACGGTTACTCAAAATTCCTGTTTGATGAATATGTTc                                                  <  2:2100892/93‑1 (MQ=255)
                                          gACGCACCTCCGACTTTATTGAATCCCGCGAGTACGAAAAACCGTTGAGCGTCTACGGTTACTCAAAATTCCTGTTTGATGAATATGTTCGTc                                               <  1:112368/93‑1 (MQ=255)
                                           aCGCACCTCCGACTTTATTGAATCCCGCGAGTACGAAAAACCGTTGAGCGTCTACGGTTACTCAAAATTCCTGTTTGATGAATATGTTCGTCa                                              <  1:3081339/93‑1 (MQ=255)
                                             gCACCTCCGACTTTATTGAATCCCGCGAGTACGAAAAACCGTTGAGCGTCTACGGTTACTCAAAATTCCTGTTTGATGAATATGTTCGTCaaa                                            <  1:316735/93‑1 (MQ=255)
                                              cACCTCCGACTTTATTGAATCCCGCGAGTACGAAAAACCGTTGAGCGTCTACGGTTACTCAAAATTCCTGTTTGATGAATATGTTCGTCAAAt                                           >  1:4582417/1‑93 (MQ=255)
                                              cACCTCCGACTTTATTGAATCCCGCGAGTACGAAAAACCGTTGAGCGTCTACGGTTACTCAAAATTCCTGTTTGATGAATATGTTCGTCAAAt                                           <  2:3706222/93‑1 (MQ=255)
                                               aCCTCCGACTTTATTGAATCCCGCGAGTACGAAAAACCGTTGAGCGTCTACGGTTACTCAAAATTCCTGTTTGATGAATATGTTCGTCAAATc                                          <  2:467329/93‑1 (MQ=255)
                                                ccTCCGACTTTATTGAATCCCGCGAGTACGAAAAACCGTTGAGCGTCTACGGTTACTCAAAATTCCTGTTTGATGAATATGTTCGTCAAATcc                                         >  1:4183175/1‑93 (MQ=255)
                                                 cTCCGACTTTATTGAATCCCGCGAGTACGAAAAACCGTTGAGCGTCTACGGTTACTCAAAATTCCTGTTTGATGAATATGTTCGTCAAATcct                                        >  1:1017000/1‑93 (MQ=255)
                                                  tCCGACTTTATTGAATCCCGCGAGTACGAAAAACCGTTGAGCGTCTACGGTTACTCAAAATTCCTGTTTGATGAATATGTTCGTCAAATcctg                                       >  2:4165378/1‑93 (MQ=255)
                                                  tCCGACTTTATTGAATCCCGCGAGTACGAAAAACCGTTGAGCGTCTACGGTTACTCAAAATTCCTGTTTGATGAATATGTTCGTCAAATcctg                                       >  2:1552515/1‑93 (MQ=255)
                                                    cGACTTTATTGAATCCCGCGAGTACGAAAAACCGTTGAGCGTCTACGGTTACTCAAAATTCCTGTTTGATGAATATGTTCGTCAAATcctgcc                                     <  1:969834/93‑1 (MQ=255)
                                                     gACTTTATTGAATCCCGCGAGTACGAAAAACCGTTGAGCGTCTACGGTTACTCAAAATTCCTGTTTGATGAATATGTTCGTCAAATcctgcct                                    >  1:2174156/1‑93 (MQ=255)
                                                         ttATTGAATCCCGCGAGTACGAAAAACCGTTGAGCGTCTACGGTTACTCAAAATTCCTGTTTGATGAATATGTTCGTCAAATCCTGCCTGAAg                                >  2:1074505/1‑93 (MQ=255)
                                                            ttGAATCCCGCGAGTACGAAAAACCGTTGAGCGTCTACGGTTACTCAAAATTCCTGTTTGATGAATATGTTCGTCAAATCCTGCCTGAAGCGa                             <  1:3923319/93‑1 (MQ=255)
                                                             tGAATCCCGCGAGTACGAAAAACCGTTGAGCGTCTACGGTTACTCAAAATTCCTGTTTGATGAATATGTTCGTCAAATCCTGCCTGAAGCGaa                            <  1:1653097/93‑1 (MQ=255)
                                                               aaTCCCGCGAGTACGAAAAACCGTTGAGCGTCTACGGTTACTCAAAATTCCTGTTTGATGAATATGTTCGTCAAATCCTGCCTGAAGCGCACt                          >  2:2435646/1‑93 (MQ=255)
                                                               aaTCCCGCGAGTACGAAAAACCGTTGAGCGTCTACGGTTACTCAAAATTCCTGTTTGATGAATATGTTCGTCAAATCCTGCCTGAAGCGAACt                          <  2:1240605/93‑1 (MQ=255)
                                                               aaTCCCGCGAGTACGAAAAACCGTTGAGCGTCTACGGTTACTCAAAATTCCTGTTTGATGAATATGTTCGTCAAATCCTGCCTGAAGCGAACt                          <  2:4340682/93‑1 (MQ=255)
                                                               aaTCCCGCGAGTACGAAAAACCGTTGAGCGTCTACGGTTACTCAAAATTCCTGTTTGATGAATATGTTCGTCAAATCCTGCCTGAAGCGAACt                          <  1:1545627/93‑1 (MQ=255)
                                                               aaTCCCGCGAGTACGAAAAACCGTTGAGCGTCTACGGTTACTCAAAATTCCTGTTTGATGAATATGTTCGTCAAATCCTGCCTGAAGCGAACt                          <  2:2605640/93‑1 (MQ=255)
                                                                aTCCCGCGAGTACGAAAAACCGTTGAGCGTCTACGGTTACTCAAAATTCCTGTTTGATGAATATGTTCGTCAAATCCTGCCTGAAGCGAACTc                         >  2:618644/1‑93 (MQ=255)
                                                                aTCCCGCGAGTACGAAAAACCGTTGAGCGTCTACGGTTACTCAAAATTCCTGTTTGATGAATATGTTCGTCAAATCCTGCCTGAAGCGAACTc                         >  1:4535290/1‑93 (MQ=255)
                                                                aTCCCGCGAGTACGAAAAACCGTTGAGCGTCTACGGTTACTCAAAATTCCTGTTTGATGAATATGTTCGTCAAATCCTGCCTGAAGCGAACTc                         >  1:3611612/1‑93 (MQ=255)
                                                                aTCCCGCGAGTACGAAAAACCGTTGAGCGTCTACGGTTACTCAAAATTCCTGTTTGATGAATATGTTCGTCAAATCCTGCCTGAAGCGAACTc                         >  1:2059810/1‑93 (MQ=255)
                                                                 tCCCGCGAGTACGAAAAACCGTTGAGCGTCTACGGTTACTCAAAATTCCTGTTTGATGAATATGTTCGTCAAATCCTGCCTGAAGCGAACTCg                        >  2:402847/1‑93 (MQ=255)
                                                                 tCCCGCGAGTACGAAAAACCGTTGAGCGTCTACGGTTACTCAAAATTCCTGTTTGATGAATATGTTCGTCAAATCCTGCCTGAAGCGAACTCg                        >  2:3771966/1‑93 (MQ=255)
                                                                  cccGCGAGTACGAAAAACCGTTGAGCGTCTACGGTTACTCAAAATTCCTGTTTGATGCATATGTTCGTCAAATCCTGCCTGCAGCGACCTCGc                       >  1:2300468/1‑93 (MQ=255)
                                                                   ccGCGAGTACGAAAAACCGTTGAGCGTCTACGGTTACTCAAAATTCCTGTTTGATGAATATGTTCGTCAAATCCTGCCTGAAGCGAACTCGCa                      <  2:48008/93‑1 (MQ=255)
                                                                   ccGCGAGTACGAAAAACCGTTGAGCGTCTACGGTTACTCAAAATTCCTGTTTGATGAATATGTTCGTCAAATCCTGCCTGAAGCGAACTCGCa                      <  1:1982164/93‑1 (MQ=255)
                                                                     gcgAGTACGAAAAACCGTTGAGCGTCTACGGTTACTCAAAATTCCTGTTTGATGAATATGTTCGTCAAATCCTGCCTGAAGCGAACTCGCAGa                    <  1:2902693/93‑1 (MQ=255)
                                                                      cgAGTACGAAAAACCGTTGAGCGTCTACGGTTACTCAAAATTCCTGTTTGATGAATATGTTCGTCAAATCCTGCCTGAAGCGAACTCGCAGAt                   <  1:3792901/93‑1 (MQ=255)
                                                                      cgAGAACGAAAAACCGTTGATCGTCTACGGTTACTCAAAATTCCTGTTTGATGAATATGTTCGTCAAATCCTGCCTGAAGCGAACTCGCAGAt                   <  2:1896946/93‑1 (MQ=255)
                                                                       gAGTACGAAAAACCGTTGAGCGTCTACGGTTACTCAAAATTCCTGTTTGATGAATATGTTCGTCAAATCCTGCCTGAAGCGAACTCGCAGAtt                  <  1:1711705/93‑1 (MQ=255)
                                                                        aGTACGAAAAACCGTTGAGCGTCTACGGTTACTCAAAATTCCTGTTTGATGAATATGTTCGTCAAATCCTGCCTGAAGCGAACTCGCAGAttg                 >  1:2937088/1‑93 (MQ=255)
                                                                         gTACGAAAAACCGTTGAGCGTCTACGGTTACTCAAAATTCCTGTTTGATGAATATGTTCGTCAAATCCTGCCTGAAGCGAACTCGCAGAttgt                <  1:1899368/93‑1 (MQ=255)
                                                                          tACGAAAAACCGTTGAGCGTCTACGGTTACTCAAAATTCCTGTTTGATGAATATGTTCGTCAAATCCTGCCTGAAGCGAACTCGCAGAttgtt               <  2:3476621/93‑1 (MQ=255)
                                                                          tACGAAAAACCGTTGAGCGTCTACGGTTACTCAAAATTCCTGTTTGATGAATATGTTCGTCAAATCCTGCCTGAAGCGAACTCGCAGAttgtt               <  2:540525/93‑1 (MQ=255)
                                                                          tACGAAAAACCGTTGAGCGTCTACGGTTACTCAAAATTCCTGTTTGATGAATATGTTCGTCAAATCCTGCCTGAAGCGAACTCGCAGAttgtt               <  1:3366480/93‑1 (MQ=255)
                                                                          tACGAAAAACCGTTGAGCGTCTACGGTTACTCAAAATTCCTGTTTGATGAATATGTTCGTCAAATCCTGCCTGAAGCGAACTCGCAGAttgtt               >  1:4644923/1‑93 (MQ=255)
                                                                          tACGAAAAACCGTTGAGCGTCTACGGTTACTCAAAATTCCTGTTTGATGAATATGTTCGTCAAATCCTGCCTGAAGCGAACTCGCAGAttgtt               >  2:2768383/1‑93 (MQ=255)
                                                                           aCGAAAAACCGTTGAGCGTCTACGGTTACTCAAAATTCCTGTTTGATGAATATGTTCGTCAAATCCTGCCTGAAGCGAACTCGCAGAttgttg              <  1:3490123/93‑1 (MQ=255)
                                                                             gAAAAACCGTTGAGCGTCTACGGTTACTCAAAATTCCTGTTTGATGAATATGTTCGTCAAATCCTGCCTGAAGCGAACTCGCAGATTGTTGGc            <  1:1321814/93‑1 (MQ=255)
                                                                              aaaaaCCGTTGAGCGTCTACGGTTACTCAAAATTCCTGTTTGATGAATATGTTCGTCAAATCCTGCCTGAAGCGAACTCGCAGATTGTTGGCt           >  1:1336683/1‑93 (MQ=255)
                                                                                   ccGTTGAGCGTCTACGGTTACTCAAAATTCCTGTTTGATGAATATGTTCGTCAAATCCTGCCTGAAGCGAACTCGCAGATTGTTGGCTTCCGt      <  2:204559/93‑1 (MQ=255)
                                                                                     gTTGAGCGTCTGCGGTTACTCAAAATTCCTGTTTGATGAATATGTTCGTCAAATCCTGCCTGAAGCGAACTCGCAGATTGTTGGCTTCCGTTa    <  1:656165/93‑1 (MQ=255)
                                                                                     gTTGAGCGTCTACGGTTACTCAAAATTCCTGTTTGATGAATATGTTCGTCAAATCCTGCCTGAAGCGAACTCGCAGATTGTTGGCTTCCGTTa    <  1:4471475/93‑1 (MQ=255)
                                                                                     gTTGAGCGTCTACGGTTACTCAAAATTCCTGTTTGATGAATATGTTCGTCAAATCCTGCCTGAAGCGAACTCGCAGATTGTTGGCTTCCGTTa    >  1:1922125/1‑93 (MQ=255)
                                                                                       tGAGCGTCTACGGTTACTCAAAATTCCTGTTTGATGAATATGTTCGTCAAATCCTGCCTGAAGCGAACTCGCAGATTGTTGGCTTCCGTTAtt  <  2:299768/93‑1 (MQ=255)
                                                                                       tGAGCGTCTACGGTTACTCAAAATTCCTGTTTGATGAATATGTTCGTCAAATCCTGCCTGAAGCGAACTCGCAGATTGTTGGCTTCCGTTAtt  <  2:3835922/93‑1 (MQ=255)
                                                                                          |                                                                                         
AAATCCCGTTCCTGTACGCTTCTTCCGCAGCCACCTACGGCGGACGCACCTCCGACTTTATTGAATCCCGCGAGTACGAAAAACCGTTGAACGTCTACGGTTACTCAAAATTCCTGTTTGATGAATATGTTCGTCAAATCCTGCCTGAAGCGAACTCGCAGATTGTTGGCTTCCGTTATT  >  NC_000913/3791936‑3792115

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GTGAAATCCCGTTCCTGTACGCTTCTTCCGCAGCCACCTACGGCGGACGCACCTCCGACTTTATTGAATCCCGCGAGTACGAAAAACCGTTGAACGTCTACGGTTACTCAAAATTCCTGTTTGATGAATATGTTCGTCAAATCCTGCCTGAAGCGAACTCGCAGATTGTTGGCTTCCGTTATTTCA  >  NC_000913/3791933‑3792118
                                                                                             |                                                                                            
GTGAAATCCCGTTCCTGTACGCTTCTTCCGCAGCCACCTACGGCGGACGCACCTCCGACTTTATTGAATCCCGCGAGTACGAAAAACCGTTGAG                                                                                              <  SRR4302535.2339431/94‑1 (MQ=60)
GTGAAATCCCGTTCCTGTACGCTTCTTCCGCAGCCACCTACGGCGGACGCACCTCCGACTTTATTGAATCCCGCGAGTACGAAAAACCGTTGAG                                                                                              >  SRR4302535.2373259/1‑94 (MQ=60)
GTGAAATCCCGTTCCTGTACGCTTCTTCCGCAGCCACCTACGGCGGACGCACCTCCGACTTTATTGAATCCCGCGAGTACGAAAAACCGTTGAG                                                                                              >  SRR4302535.4717454/1‑94 (MQ=60)
GTGAAATCCCGTTCCTGTACGCTTCTTCCGCAGCCACCTACGGCGGACGCACCTCCGACTTTATTGAATCCCGCGAGTACGAAAAACCGTTGAG                                                                                              <  SRR4302535.4691800/94‑1 (MQ=60)
GTGAAATCCCGTTCCTGTACGCTTCTTCCGCAGCCACCTACGGCGGACGCACCTCCGACTTTATTGAATCCCACGAGTACGAAAAACCGTTGAG                                                                                              >  SRR4302535.254355/1‑94 (MQ=60)
 TGAAATCCCGTTCCTGTACGCTTCTTCCGCAGCCACCTACGGCGGACGCACCTCCGACTTTATTGAATCCCGCGAGTACGAAAAACCGTTGAGC                                                                                             <  SRR4302535.3304648/94‑1 (MQ=60)
 TGAAATCCCGTTCCTGTACGCTTCTTCCGCAGCCACCTACGGCGGACGCACCTCCGACTTTATTGAATCCCGCGAGTACGAAAAACCGTTGAGC                                                                                             <  SRR4302535.1639169/94‑1 (MQ=60)
 TGAAATCCCGTTCCTGTACGCTTCTTCCGCAGCCACCTACGGCGGACGCACCTCCGACTTTATTGAATCCCGCGAGTACGAAAAACCGTTGAGC                                                                                             >  SRR4302535.3673629/1‑94 (MQ=60)
 TGAAATCCCGTTCCTGTACGCTTCTTCCGCAGCCACCTACGGCGGACGCACCTCCGACTTTATTGAATCCCGCGAGTACGAAAAACCGTTGAGC                                                                                             <  SRR4302535.3761399/94‑1 (MQ=60)
  GAAATCCCGTTCCTGTACGCTTCTTCCGCAGCCACCTACGGCGGACGCACCTCCGACTTTATTGAATCCCGCGAGTACGAAAAACCGTTGAGCG                                                                                            >  SRR4302535.863568/1‑94 (MQ=60)
  GAAATCCCGTTCCTGTACGCTTCTTCCGCAGCCACCTACGGCGGACGCACCTCCGACTTTATTGAATCCCGCGAGTACGAAAAACCGTTGAGCG                                                                                            <  SRR4302535.352230/94‑1 (MQ=60)
   AAATCCCGTTCCTGTACGCTTCTTCCGCAGCCACCTACGGCGGACGCACCTCCGACTTTATTGAATCCCGCGAGTACGAAAAACCGTTGAGCGT                                                                                           <  SRR4302535.3662976/94‑1 (MQ=60)
   AAATCCCGTTCCTGTACGCTTCTTCCGCAGCCACCTACGGCGGACGCACCTCCGACTTTATTGAATCCCGCGAGTACGAAAAACCGTTGAGCGT                                                                                           >  SRR4302535.1805387/1‑94 (MQ=60)
    AATCCCGTTCCTGTACGCTTCTTCCGCAGCCACCTACGGCGGACGCACCTCCGACTTTATTGAATCCCGCGAGTACGAAAAACCGTTGAGCGTC                                                                                          >  SRR4302535.2988903/1‑94 (MQ=60)
    AATCCCGTTCCTGTACGCTTCTTCCGCAGCCACCTACGGCGGACGCACCTCCGACTTTATTGAATCCCGCGAGTACGAAAAACCGTTGAGCGTC                                                                                          <  SRR4302535.2544997/94‑1 (MQ=60)
     ATCCCGTTCCTGTACGCTTCTTCCGCAGCCACCTACGGCGGACGCACCTCCGACTTTATTGAATCCCGCGAGTACGAAAAACCGTTGAGCGTCT                                                                                         <  SRR4302535.3088323/94‑1 (MQ=60)
     ATCCCGTTCCTGTACGCTTCTTCCGCAGCCACCTACGGCGGACGCACCTCCGACTTTATTGAATCCCGCGAGTACGAAAAACCGTTGAGCGTCT                                                                                         >  SRR4302535.4205482/1‑94 (MQ=60)
     ATCCCGTTCCTGTACGCTTCTTCCGCAGCCACCTACGGCGGACGCACCTCCGACTTTATTGAATCCCGCGAGTACGAAAAACCGTTGAGCGTCT                                                                                         >  SRR4302535.4013355/1‑94 (MQ=60)
     ATCCCGTTCCTGTACGCTTCTTCCGCAGCCACCTACGGCGGACGCACCTCCGACTTTATTGAATCCCGCGAGTACGAAAAACCGTTGAGCGTCT                                                                                         <  SRR4302535.2346657/94‑1 (MQ=60)
      TCCCGTTCCTGTACGCTTCTTCCGCAGCCACCTACGGCGGACGCACCTCCGACTTTATTGAATCCCGCGAGTACGAAAAACCGTTGAGCGTCTA                                                                                        >  SRR4302535.1709575/1‑94 (MQ=60)
       CCCGTTCCTGTACGCTTCTTCCGCAGCCACCTACGGCGGACGCACCTCCGACTTTATTGAATCCCGCGAGTACGAAAAACCGTTGAGCGTCTAC                                                                                       >  SRR4302535.1281732/1‑94 (MQ=60)
       CCCGTTCCTGTACGCTTCTTCCGCAGCCACCTACGGCGGACGCACCTCCGACTTTATTGAATCCCGCGAGTACGAAAAACCGTTGAGCGTCTAC                                                                                       <  SRR4302535.4690278/94‑1 (MQ=60)
       CCCGTTCCTGTACGCTTCTTCCGCAGCCACCTACGGCGGACGCACCTCCGACTTTATTGAATCACGCGAGTACGAAAAACCGTTGAGCGTCTAC                                                                                       >  SRR4302535.2369142/1‑94 (MQ=60)
        CCGTTCCTGTACGCTTCTTCCGCAGCCACCTACGGCGGACGCACCTCCGACTTTATTGAATCCCGCGAGTACGAAAAACCGTTGAGCGTCTACG                                                                                      <  SRR4302535.896974/94‑1 (MQ=60)
         CGTTCCTGTACGCTTCTTCCGCAGCCACCTACGGCGGACGCACCTCCGACTTTATTGAATCCCGCGAGTACGAAAAACCGTTGAGCGGCTACGG                                                                                     >  SRR4302535.186998/1‑94 (MQ=60)
          GTTCCTGTACGCTTCTTCCGCAGCCACCTACGGCGGACGCACCTCCGACTTTATTGAATCCCGCGAGTACGAAAAACCGTTGAGCGTCTACGGT                                                                                    >  SRR4302535.4580432/1‑94 (MQ=60)
          GTTCCTGTACGCTTCTTCCGCAGCCACCTACGGCGGACGCACCTCCGACTTTATTGAATCCCGCGAGTACGAAAAACCGTTGAGCGTCTACGGT                                                                                    >  SRR4302535.2857182/1‑94 (MQ=60)
          GTTCCTGTACGCTTCTTCCGCAGCCACCTACGGCGGACGCACCTCCGACTTTATTGAATCCCGCGAGTACGAAAAACCGTTGAGCGTCTACGGT                                                                                    >  SRR4302535.4094936/1‑94 (MQ=60)
          GTTCCTGTACGCTTCTTCCGCAGCCACCTACGGCGGACGCACCTCCGACTTTATTGAATCCCGCGAGTACGAAAAACCGTTGAGCGTCTACGGT                                                                                    >  SRR4302535.3975770/1‑94 (MQ=60)
           TTCCTGTACGCTTCTTCCGCAGCCACCTACGGCGGACGCACCTCCGACTTTATTGAATCCCGCGAGTACGAAAAACCGTTGAGCGTCTACGGTT                                                                                   <  SRR4302535.3559991/94‑1 (MQ=60)
           TTCCTGTACGCTTCTTCCGCAGCCACCTACGGCGGACGCACCTCCGACTTTATTGAATCCCGCGAGTACGAAAAACCGTTGAGCGTCTACGGTT                                                                                   <  SRR4302535.1077251/94‑1 (MQ=60)
           TTCCTGTACGCTTCTTCCGCAGCCACCTACGGCGGACGCACCTCCGACTTTATTGAATCCCGCGAGTACGAAAAACCGTTGAGCGTCTACGGTT                                                                                   <  SRR4302535.2862283/94‑1 (MQ=60)
           TTCCTGTACGCTTCTTCCGCAGCCACCTACGGCGGACGCACCTCCGACTTTATTGAATCCCGCGAGTACGAAAAACCGTTGAGCGTCTACGGTT                                                                                   >  SRR4302535.77118/1‑94 (MQ=60)
             CCTGTACGCTTCTTCCGCAGCCACCTACGGCGGACGCACCTCCGACTTTATTGAATCCCGCGAGTACGAAAAACCGTTGAGCGTCTACGGTTAC                                                                                 <  SRR4302535.2351436/94‑1 (MQ=60)
              CTGTACGCTTCTTCCGCAGCCACCTACGGCGGACGCACCTCCGACTTTATTGAATCCCGCGAGTACGAAAAACCGTTGAGCGTCTACGGTTACT                                                                                >  SRR4302535.3264669/1‑94 (MQ=60)
              CTGTACGCTTCTTCCGCAGCCACCTACGGCGGACGCACCTCCGACTTTATTGAATCCCGCGAGTACGAAAAACCGTTGAGCGTCTACGGTTACT                                                                                >  SRR4302535.1102021/1‑94 (MQ=60)
              CTGTACGCTTCTTCCGCAGCCACCTACGGCGGACGCACCTCCGAATTTATTGAATCCCGCGAGTACGAAAAACCGTTGAGCGTCTACGGTTACT                                                                                >  SRR4302535.788437/1‑94 (MQ=60)
                GTACGCTTCTTCCGCAGCCACCTACGGCGGACGCACCTCCGACTTTATTGAATCCCGCGAGTACGAAAAACCGTTGAGCGTCTACGGTTACTCA                                                                              >  SRR4302535.1790610/1‑94 (MQ=60)
                GTACGCTTCTTCCGCAGCCACCTACGGCGGACGCACCTCCGACTTTATTGAATCCCGCGAGTACGAAAAACCGTTGAGCGTCTACGGTTACTCA                                                                              >  SRR4302535.626264/1‑94 (MQ=60)
                  ACGCTTCTTCCGCAGCCACCTACGGCGGACGCACCTCCGACTTTATTGAATCCCGCGAGTACGAAAAACCGTTGAGCGTCTACGGTTACTCAAA                                                                            >  SRR4302535.4202673/1‑94 (MQ=60)
                   CGCTTCTTCCGCAGCCACCTACGGCGGACGCACCTCCGACTTTATTGAATCCCGCGAGTACGAAAAACCGTTGAGCGTCTACGGTTACTCAAAA                                                                           >  SRR4302535.1890313/1‑94 (MQ=60)
                    GCTTCTTCCGCAGCCACCTACGGCGGACGCACCTCCGACTTTATTGAATCCCGCNAGTACGAANAACCGTTNAGCGTCTACGGTTNCNCNANAT                                                                          >  SRR4302535.1119203/1‑94 (MQ=60)
                    GCTTCTTCCGCAGCCACCTACGGCGGACGCACCTCCGACTTTATTGAATCCCGCGAGTACGAAAAACCGTTGAGCGTCTACGGTTACTCAAAAT                                                                          <  SRR4302535.2557265/94‑1 (MQ=60)
                    GCTTCTTCCGCAGCCACCTACGGCGGACGCACCTCCGACTTTATTGAATCCCGCGAGTACGAAAAACCGTTGAGCGTCTACGGTTACTCAAAAT                                                                          <  SRR4302535.1509249/94‑1 (MQ=60)
                    GCTTCTTCCGCAGCCACCTACGGCGGACGCACCTCCGACTTTATTGAATCCCGCGAGTACGAAAAACCGTTGAGCGTCTACGGTTACTCAAAAT                                                                          <  SRR4302535.3503722/94‑1 (MQ=60)
                    GCTTCTTCCGCAGCCACCTACGGCGGAAGCACCTCCGACTTTATTGAATCCCGCGAGTACGAAAAACCGTTGAGCGTCTACGGTTACTCAAAAT                                                                          >  SRR4302535.1882954/1‑94 (MQ=60)
                     CTTCTTCCGCAGCCACCTACGGCGGACGCACCTCCGACTTTATTGAATCCCGCGAGTACGAAAAACCGTTGAGCGTCTACGGTTACTCAAAATT                                                                         <  SRR4302535.3823141/94‑1 (MQ=60)
                     CTTCTTCCGCAGCCACCTACGGCGGACGCACCTCCGACTTTATTGAATCCCGCGAGTACGAAAAACCGTTGAGCGTCTACGGTTACTCAAAATT                                                                         >  SRR4302535.602804/1‑94 (MQ=60)
                     CTTCTTCCGCAGCCACCTACGGCGGACGCACCTCCGACTTTATTGAATCCCGCGAGTACGAAAAACCGTTGAGCGTCTACGGTTACTCAAAATT                                                                         <  SRR4302535.43475/94‑1 (MQ=60)
                     CTTCTTCCGCAGCCACCTACGGCGGACGCACCTCCGACTTTATTGAATCCCGCGAGCACGTAAAACCGTTGTGCGTCTTCGGCTACTCCAAATT                                                                         >  SRR4302535.1391361/1‑94 (MQ=60)
                        CTTCCGCAGCCACCTACGGCGGACGCACCTCCGACTTTATTGAATCCCGCGAGTACGAAAAACCGTTGAGCGTCTACGGTTACTCAAAATTCCT                                                                      <  SRR4302535.2589132/94‑1 (MQ=60)
                        CTTCCGCAGCCACCTACGGCGGACGCACCGCCGACTTTATTGAATCCCGCGAGTACGAAAAACCGTTGAGCGTCTACGGTTACTCAAAATTCCT                                                                      <  SRR4302535.1922030/94‑1 (MQ=60)
                         TTCCGCAGCCACCTACGGCGGACGCACCTCCGACTTTATTGAATCCCGCGAGTACGAAAAACCGTTGAGCGTCTACGGTTACTCAAAATTCCTG                                                                     <  SRR4302535.485681/94‑1 (MQ=60)
                          TCCGCAGCCACCTACGGCGGACGCACCTCCGACTTTATTGAATCCCGCGAGTACGAAAAACCGTTGAGCGTCTACGGTTACTCAAAATTCCTGT                                                                    <  SRR4302535.424837/94‑1 (MQ=60)
                           CCGCAGCCACCTACGGCGGACGCACCTCCGACTTTATTGAATCCCGCGAGTACGAAAAACCGTTGAGCGTCTACGGTTACTCAAAATTCCTGTT                                                                   <  SRR4302535.2099425/94‑1 (MQ=60)
                           CCGCAGCCACCTACGGCGGACGCACCTCCGACTTTATTGAATCCCGCGAGTACGAAAAACCGTTGAGCGTCTACGGTTACTCAAAATTCCTGTT                                                                   <  SRR4302535.814632/94‑1 (MQ=60)
                            CGCAGCCACCTACGGCGGACGCACCTCCGACTTTATTGAATCCCGCGAGTACGAAAAACCGTTGAGCGTCTACGGTTACTCAAAATTCCTGTTT                                                                  <  SRR4302535.482577/94‑1 (MQ=60)
                            CGCAGCCACCTACGGCGGACGCACCTCCGACTTTATTGAATCCCGCGAGTACGAAAAACCGTTGAGCGTCTACGGTTACTCAAAATTCCTGTTT                                                                  <  SRR4302535.1676768/94‑1 (MQ=60)
                            CGCAGCCACCTACGGCGGACGCACCTCCGACTTTATTGAATCCCGCGAGTACGAAAAACCGTTGAGCGTCTACGGTTACTCAAAATTCCTGTTT                                                                  <  SRR4302535.4181506/94‑1 (MQ=60)
                            CGCAGCCACCTACGGCGGACGCACCTCCGACTTTATTGAATCCCGCGAGTACGAAAAACCGTTGAGCGTCTACGGTTACTCAAAATTCCTGTTT                                                                  >  SRR4302535.3465977/1‑94 (MQ=60)
                             GCAGCCACCTACGGCGGACGCACCTCCGACTTTATTGAATCCCGCGAGTACGAAAAACCGTTGAGCGTCTACGGTTACTCAAAATTCCTGTTTG                                                                 <  SRR4302535.56886/94‑1 (MQ=60)
                              CAGCCACCTACGGCGGACGCACCTCCGACTTTATTGAATCCCGCGAGTACGAAAAACCGTTGAGCGTCTACGGTTACTCAAAATTCCTGTTTGA                                                                <  SRR4302535.4218562/94‑1 (MQ=60)
                              CAGCCACCTACGGCGGACGCACCTCCGACTTTATTGAATCCCGCGAGTACGAAAAACCGTTGAGCGTCTACGGTTACTCAAAATTCCTGTTTGA                                                                >  SRR4302535.1551506/1‑94 (MQ=60)
                               AGCCACCTACGGCGGACGCACCTCCGACTTTATTGAATCCCGCGAGTACGAAAAACCGTTGAGCGTCTACGGTTACTCAAAATTCCTGTTTGAT                                                               >  SRR4302535.2681814/1‑94 (MQ=60)
                               AGCCACCTACGGCGGACGCACCTCCGACTTTATTGAATCCCGCGAGTACGAAAAACCGTTGAGCGTCTACGGTTACTCAAAATTCCTGTTTGAT                                                               <  SRR4302535.1249128/94‑1 (MQ=60)
                               AGCCACCTACGGCGGACGCACCTCCGACTTTATTGAATCCCGCGAGTACGAAAAACCGTTGAGCGTCTACGGTTACTCAAAATTCCTGTTTGAT                                                               >  SRR4302535.3852014/1‑94 (MQ=60)
                                GCCACCTACGGCGGACGCACCTCCGACTTTATTGAATCCCGCGAGTACGAAAAACCGTTGAGCGTCTACGGTTACTCAAAATTCCTGTTTGATG                                                              <  SRR4302535.3441526/94‑1 (MQ=60)
                                GCCACCTACGGCGGACGCACCTCCGACTTTATTGAATCCCGCGAGTACGAAAAACCGTTGAGCGTCTACGGTTACTCAAAATTCCTGTTTGATG                                                              <  SRR4302535.4510838/94‑1 (MQ=60)
                                   ACCTACGGCGGACGCACCTCCGACTTTATTGAATCCCGCGAGTACGAAAAACCGTTGAGCGTCTACGGTTACTCAAAATTCCTGTTTGATGAAT                                                           <  SRR4302535.1660145/94‑1 (MQ=60)
                                   ACCTACGGCGGACGCACCTCCGACTTTATTGAATCCCGCGAGTACGAAAAACCGTTGAGCGTCTACGGTTACTCAAAATTCCTGTTTGATGAAT                                                           >  SRR4302535.3259822/1‑94 (MQ=60)
                                    CCTACGGCGGACGCACCTCCGACTTTATTGAATCCCGCGAGTACGAAAAACCGTTGAGCGTCTACGGTTACTCAAAATTCCTGTTTGATGAATA                                                          >  SRR4302535.274527/1‑94 (MQ=60)
                                    CCTACGGCGGACGCACCTCCGACTTTATTGAATCCCGCGAGTACGAAAAACCGTTGAGCGTCTACGGTTACTCAAAATTCCTGTTTGATGAATA                                                          >  SRR4302535.3009369/1‑94 (MQ=60)
                                    CCTACGGCGGACGCACCTCCGACTTTATTGAATCCCGCGAGTACGAAAAACCGTTGAGCGTCTACGGTTACTCAAAATTCCTGTTTGATGAATA                                                          >  SRR4302535.1114627/1‑94 (MQ=60)
                                     CTGCGGCGGACGCACATCCGACTTTATAGAATCCCGCGGGTACGAAAAACCGTTGAGCGCCTCTGGTTACTCAAAATTCCTGTTTGATGAATAT                                                         <  SRR4302535.1992528/94‑1 (MQ=60)
                                     CTACGGCGGACGCACCTCCGACTTTATTGAATCCCGCGAGTATGAAAAACCGTTGAGCGTCTACGGTTACTCAAAATTCCTGTTTGATGAATAT                                                         <  SRR4302535.1766438/94‑1 (MQ=60)
                                     CTACGGCGGACGCACCTCCGACTTTATTGAATCCCGCGAGTACGAAAAACCGTTGAGCGTCTACGGTTACTCAAAATTCCTGTTTGATGCATAT                                                         >  SRR4302535.2959090/1‑94 (MQ=60)
                                     CTACGGCGGACGCACCTCCGACTTTATTGAATCCCGCGAGTACGAAAAACCGTTGAGCGTCTACGGTTACTCAAAATTCCTGTTTGATGAATAT                                                         <  SRR4302535.1336620/94‑1 (MQ=60)
                                     CTACGGCGGACGCACCTCCGACTTTATTGAATCCCGCGAGTACGAAAAACCGTTCAGCGTCTACGGTTACTCAAAATTCCTGTTTGATGAATAT                                                         >  SRR4302535.4442085/1‑94 (MQ=60)
                                        CGGCGGACGCACCTCCGACTTTATTGAATCCCGCGAGTACGAAAAACCGTTGAGCGTCTACGGTTACTCAAAATTCCTGTTTGATGAATATGTT                                                      <  SRR4302535.2154157/94‑1 (MQ=60)
                                        CGGCGGACGCACCTCCGACTTTATTGAATCCCGCGAGTACGAAAAACCGTTGAGCGTCTACGGTTACTCAAAATTCCTGTTTGATGAATATGTT                                                      <  SRR4302535.3155101/94‑1 (MQ=60)
                                         GGCGGACGCACCTCCGACTTTATTGAATCCCGCGAGTACGAAAAACCGTTGAGCGTCTACGGTTACTCAAAATTCCTGTTTGATGAATATGTTC                                                     >  SRR4302535.4233731/1‑94 (MQ=60)
                                         GGCGGACGCACCTCCGACTTTATTGAATCCCGCGAGTACGAAAAACCGTTGAGCGTCTACGGTTACTCAAAATTCCTGTTTGATGAATATGTTC                                                     <  SRR4302535.3864510/94‑1 (MQ=60)
                                          GCGGACGCACCTCCGACTTTATTGAATCCCGCGAGTACGAAAAACCGTTGAGCGTCTACGGTTACTCAAAATTCCTGTTTGATGAATATGTTCG                                                    <  SRR4302535.2817456/94‑1 (MQ=60)
                                            GGACGCACCTCCGACTTTATTGAATCCCGCGAGTACGAAAAACCGTTGAGCGTCTACGGTTACTCAAAATTCCTGTTTGATGAATATGTTCGTC                                                  <  SRR4302535.4634387/94‑1 (MQ=60)
                                             GACGCACCTCCGACTTTATTGAATCCCGCGAGTACGAAAAACCGTTGAGCGTCTACGGTTACTCAAAATTCCTGTTTGATGAATATGTTCGTCA                                                 <  SRR4302535.1761625/94‑1 (MQ=60)
                                             GACGCACCTCCGACTTTATTGAATCCCGCGAGTACGAAAAACCGTTGAGCGTCTACGGTTACTCAAAATTCCTGTTTGATGAATATGTTCGTCA                                                 >  SRR4302535.414151/1‑94 (MQ=60)
                                             GACGCACCTCCGACTTTATTGAATCCCGCGAGTACGAAAAACCGTTGAGCGTCTACGGTTACTCAAAATTCCTGTTTGATGAATATGTTCGTCA                                                 <  SRR4302535.113159/94‑1 (MQ=60)
                                              ACGCACCTCCGACTTTATTGAATCCCGCGAGTACGAAAAACCGTTGAGCGTCTACGGTTACTCAAAATTCCTGTTTGATGAATATGTTCGTCAA                                                <  SRR4302535.3102818/94‑1 (MQ=60)
                                              ACGCACCTCCGACTTTATTGAATCCCGCGAGTACGAAAAACCGTTGAGCGTCTACGGTTACTCAAAATTCCTGTTTGATGAATATGTTCGTCAA                                                <  SRR4302535.298245/94‑1 (MQ=60)
                                                GCACCTCCGACTTTATTGAATCCCGCGAGTACGAAAAACCGTTGAGCGTCTACGGTTACTCAAAATTCCTGTTTGATGAATATGTTCGTCAAAT                                              <  SRR4302535.318934/94‑1 (MQ=60)
                                                GCACCTCCGACTTTATTGAATCCCGCGAGTACGAAAAACCGTTGAGCGTCTACGGTTACTCAAAATTCCTGTTTGATGAATATGTTCGTCAAAT                                              >  SRR4302535.4613626/1‑94 (MQ=60)
                                                 CACCTCCGACTTTATTGAATCCCGCGAGTACGAAAAACCGTTGAGCGTCTACGGTTACTCAAAATTCCTGTTTGATGAATATGTTCGTCAAATC                                             <  SRR4302535.2521679/94‑1 (MQ=60)
                                                  ACCTCCGACTTTATTGAATCCCGCGAGTACGAAAAACCGTTGAGCGTCTACGGTTACTCAAAATTCCTGTTTGATGAATATGTTCGTCAAATCC                                            >  SRR4302535.4211798/1‑94 (MQ=60)
                                                   CCTCCGACTTTATTGAATCCCGCGAGTACGAAAAACCGTTGAGCGTCTACGGTTACTCAAAATTCCTGTTTGATGAATATGTTCGTCAAATCCT                                           >  SRR4302535.1024226/1‑94 (MQ=60)
                                                   CCTCCGACTTTATTGAATCCCGCGAGTACGAAAAACCGTTGAGCGTCTACGGTTACTCAAAATTCCTGTTTGATGAATATGTTCGTCAAATCCT                                           <  SRR4302535.3892883/94‑1 (MQ=60)
                                                   CCTCCGACTTTATTGAATCCCGCGAGTACGAAAAACCGTTGAGCGTCTACGGTTACTCAAAATTCCTGTTTGATGAATATGTTCGTCAAATCCT                                           >  SRR4302535.2670682/1‑94 (MQ=60)
                                                    CTCCGACTTTATTGAATCCCGCGAGTACGAAAAACCGTTGAGCGTCTACGGTTACTCAAAATTCCTGTTTGATGAATATGTTCGTCAAATCCTG                                          >  SRR4302535.196628/1‑94 (MQ=60)
                                                      CCGACTTTATTGAATCCCGCGAGTACGAAAAACCGTTGAGCGTCTACGGTTACTCAAAATTCCTGTTTGATGAATATGTTCGTCAAATCCTGCC                                        <  SRR4302535.4687099/94‑1 (MQ=60)
                                                       CGACTTTATTGAATCCCGCGAGTACGAAAAACCGTTGAGCGTCTACGGTTACTCAAAATTCCTGTTTGATGAATATGTTCGTCAAATCCTGCCT                                       <  SRR4302535.976720/94‑1 (MQ=60)
                                                       CGACTTTATTGAATCCCGCGAGTACGAAAAACCGTTGAGCGTCTACGGTTACTCAAAATTCCTGTTTGATGAATATGTTCGTCAAATCCTGCCT                                       <  SRR4302535.2555139/94‑1 (MQ=60)
                                                       CGACTTTATTGAATCCCGCGAGTACGAAAAACCGTTGAGCGTCTACGGTTACTCAAAATTCCTGTTTGATGAATATGTTCGTCAAATCCTGCCT                                       >  SRR4302535.2189706/1‑94 (MQ=60)
                                                           TTTATTGAATCCCGCGAGTACGAAAAACCGTTGAGCGTCTACGGTTACTCAAAATTCCTGTTTGATGAATATGTTCGTCAAATCCTGCCTGAAG                                   <  SRR4302535.1283193/94‑1 (MQ=60)
                                                           TTTATTGAATCCCGCGAGTACGAAAAACCGTTGAGCGTCTACGGTTACTCAAAATTCCTGTTTGATGAATATGTTCGTCAAATCCTGCCTGAAG                                   <  SRR4302535.4531859/94‑1 (MQ=60)
                                                             TATTGAATCCCGCGAGTACGAAAACCCGTTGAGCGTCTACGGTTACTCAAAATTCCTGTTTGATGAATATGTTCGTCAAATCCTGCCTGAAGCG                                 <  SRR4302535.2052742/94‑1 (MQ=60)
                                                             TATTGAATCCCGCGAGTACGAAAAACCGTTGAGCGTCTACGGTTACTCAAAATTCCTGTTTGATGAATATGTTCGTCAAATCCTGCCTGAAGCG                                 <  SRR4302535.499481/94‑1 (MQ=60)
                                                              ATTGAATCCCGCGAGTACGAAAAACCGTTGAGCGTCTACGGTTACTCAAAATTCCTGTTTGATGAATATGTTCGTCAAATCCTGCCTGAAGCGA                                >  SRR4302535.3757128/1‑94 (MQ=60)
                                                               TTGAATCCCGCGAGTACGAAAAACCGTTGAGCGTCTACGGTTACTCAAAATTCCTGTTTGATGAATATGTTCGTCAAATCCTGCCTGAAGCGAA                               <  SRR4302535.3950131/94‑1 (MQ=60)
                                                                TGAATCCCGCGAGTACGAAAAACCGTTGAGCGTCTACGGTTACTCAAAATTCCTGTTTGATGAATATGTTCGTCAAATCCTGCCTGAAGCGAAC                              <  SRR4302535.1665060/94‑1 (MQ=60)
                                                                TGAATCCCGCGAGTACGAAAAACCGTTGAGCGTCTACGGTTACTCAAAATTCCTGTTTGATGAATATGTTCGTCAAATCCTGCCTGAAGCGAAC                              >  SRR4302535.2217498/1‑94 (MQ=60)
                                                                  AATCCCGCGAGTACGAAAAACCGTTGAGCGTCTACGGTTACTCAAAATTCCTGTTTGATGAATATGTTCGTCAAATCCTGCCTGAAGCGAACTC                            >  SRR4302535.2074651/1‑94 (MQ=60)
                                                                  AATCCCGCGAGTACGAAAAACCGTTGAGCGTCTACGGTTACTCAAAATTCCTGTTTGATGAATATGTTCGTCAAATCCTGCCTGAAGCGAACTC                            >  SRR4302535.4566323/1‑94 (MQ=60)
                                                                  AATCCCGCGAGTACGAAAAACCGTTGAGCGTCTACGGTTACTCAAAATTCCTGTTTGATGAATATGTTCGTCAAATCCTGCCTGAAGCGAACTC                            >  SRR4302535.3636657/1‑94 (MQ=60)
                                                                  AATCCCGCGAGTACGAAAAACCGTTGAGCGTCTACGGTTACTCAAAATTCCTGTTTGATGAATATGTTCGTCAAATCCTGCCTGAAGCGAACTC                            >  SRR4302535.2703684/1‑94 (MQ=60)
                                                                  AATCCCGCGAGTACGAAAAACCGTTGAGCGTCTACGGTTACTCAAAATTCCTGTTTGATGAATATGTTCGTCAAATCCTGCCTGAAGCGAACTC                            <  SRR4302535.1556757/94‑1 (MQ=60)
                                                                   ATCCCGCGAGTACGAAAAACCGTTGAGCGTCTACGGTTACTCAAAATTCCTGTTTGATGAATATGTTCGTCAAATCCTGCCTGAAGCGAACTCG                           >  SRR4302535.960177/1‑94 (MQ=60)
                                                                    TCCCGCGAGTACGAAAAACCGTTGAGCGTCTACGGTTACTCAAAATTCCTGTTTGATGCATATGTTCGTCAAATCCTGCCTGCAGCGACCTCGC                          >  SRR4302535.2316930/1‑94 (MQ=60)
                                                                    TCCCGCGAGTACGAAAAACCGTTGAGCGTCTACGGTTACTCAAAATTCCTGTTTGATGAATATGTTCGTCAAATCCTGCCTGAAGCGAACTCGC                          >  SRR4302535.1390715/1‑94 (MQ=60)
                                                                     CCCGCGAGTACGAAAAACCGTTGAGCGTCTACGGTTACTCAAAATTCCTGTTTGATGAATATGTTCGTCAAATCCTGCCTGAAGCGAACTCGCA                         >  SRR4302535.1219723/1‑94 (MQ=60)
                                                                     CCCGCGAGTACGAAAAACCGTTGAGCGTCTACGGTTACTCAAAATTCCTGTTTGATGAATATGTTCGTCAAATCCTGCCTGAAGCGAACTCGCA                         >  SRR4302535.3973047/1‑94 (MQ=60)
                                                                     CCCGCGAGTACGAAAAACCGTTGAGCGTCTACGGTTACTCAAAATTCCTGTTTGATGAATATGTTCGTCAAATCCTGCCTGAAGCGAACTCGCA                         >  SRR4302535.2283197/1‑94 (MQ=60)
                                                                      CCGCGAGTACGAAAAACCGTTGAGCGTCTACGGTTACTCAAAATTCCTGTTTGATGAATATGTTCGTCAAATCCTGCCTGAAGCGAACTCGCAG                        <  SRR4302535.3792828/94‑1 (MQ=60)
                                                                      CCGCGAGTACGAAAAACCGTTGAGCGTCTACGGTTACTCAAAATTCCTGTTTGATGAATATGTTCGTCAAATCCTGCCTGAAGCGAACTCGCAG                        <  SRR4302535.1996435/94‑1 (MQ=60)
                                                                      CCGCGAGTACGAAAAACCGTTGAGCGTCTACGGTTACTCAAAATTCCTGTTTGATGAATATGTTCGTCAAATCCTGCCTGAAGCGAACTCGCAG                        >  SRR4302535.3468825/1‑94 (MQ=60)
                                                                      CCGCGAGTACGAAAAACCGTTGAGCGTCTACGGTTACTCAAAATTCCTGTTTGATGAATATGTTCGTCAAATCCTGCCTGAAGCGAACTCGCAG                        <  SRR4302535.1535358/94‑1 (MQ=60)
                                                                       CGCGAGTACGAAAAACCGTTGAGCGTCTACGGTTACTCAAAATTCCTGTTTGATGAATATGTTCGTCAAATCCTGCCTGAAGCGAACTCGCAGA                       >  SRR4302535.1585399/1‑94 (MQ=60)
                                                                        GCGAGTACGAAAAACCGTTGAGCGTCTACGGTTACTCAAAATTCCTGTTTGATGAATATGTTCGTCAAATCCTGCCTGAAGCGAACTCGCAGAT                      <  SRR4302535.2923211/94‑1 (MQ=60)
                                                                         CGAGTACGAAAAACCGTTGAGCGTCTACGGTTACTCAAAATTCCTGTTTGATGAATATGTTCGTCAAATCCTGCCTGAAGCGAACTCGCAGATT                     <  SRR4302535.3819050/94‑1 (MQ=60)
                                                                          GAGTACGAAAAACCGTTGAGCGTCTACGGTTACTCAAAATTCCTGTTTGATGAATATGTTCGTCAAATCCTGCCTGAAGCGAACTCGCAGATTG                    >  SRR4302535.2957752/1‑94 (MQ=60)
                                                                          GAGTACGAAAAACCGTTGAGCGTCTACGGTTACTCAAAATTCCTGTTTGATGAATATGTTCGTCAAATCCTGCCTGAAGCGAACTCGCAGATTG                    <  SRR4302535.1724084/94‑1 (MQ=60)
                                                                           AGTACGAAAAACCGTTGAGCGTCTACGGTTACTCAAAATTCCTGTTTGATGAATATGTTCGTCAAATCCTGCCTGAAGCGAACTCGCAGATTGT                   >  SRR4302535.4003380/1‑94 (MQ=60)
                                                                            GTACGAAAAACCGTTGAGCGTCTACGGTTACTCAAAATTCCTGTTTGATGAATATGTTCGTCAAATCCTGCCTGAAGCGAACTCGCAGATTGTT                  >  SRR4302535.4676401/1‑94 (MQ=60)
                                                                            GTACGAAAAACCGTTGAGCGTCTACGGTTACTCAAAATTCCTGTTTGATGAATATGTTCGTCAAATCCTGCCTGAAGCGAACTCGCAGATTGTT                  <  SRR4302535.1913038/94‑1 (MQ=60)
                                                                            GTACGAAAAACCGTTGAGCGTCTACGGTTACTCAAAATTCCTGTTTGATGAATATGTTCGTCAAATCCTGCCTGAAGCGAACTCGCAGATTGTT                  >  SRR4302535.3162750/1‑94 (MQ=60)
                                                                             TACGAAAAACCGTTGAGCGTCTACGGTTACTCAAAATTCCTGTTTGATGAATATGTTCGTCAAATCCTGCCTGAAGCGAACTCGCAGATTGTTG                 <  SRR4302535.3389905/94‑1 (MQ=60)
                                                                              ACGAAAAACCGTTGAGCGTCTACGGTTACTCAAAATTCCTGTTTGATGAATATGTTCGTCAAATCCTGCCTGAAGCGAACTCGCAGATTGTTGG                <  SRR4302535.3514375/94‑1 (MQ=60)
                                                                              ACGAAAAACCGTTGAGCGTCTACGGTTACTCAAAATTCCTGTTTGATGAATATGTTCGTCAAATCCTGCCTGAAGCGAACTCGCAGATTGTTGG                >  SRR4302535.396866/1‑94 (MQ=60)
                                                                               CGAAAAACCGTTGAGCGTCTACGGTTACTCAAAATTCCTGTTTGATGAATATGTTCGTCAAATCCTGCCTGAAGCGAACTCGCAGATTGTTGGC               <  SRR4302535.122182/94‑1 (MQ=60)
                                                                                GAAAAACCGTTGAGCGTCTACGGTTACTCAAAATTCCTGTTTGATGAATATGTTCGTCAAATCCTGCCTGAAGCGAACTCGCAGATTGTTGGCT              <  SRR4302535.1331250/94‑1 (MQ=60)
                                                                                GAAAAACCGTTGAGCGTCTACGGTTACTCAAAATTCCTGTTTGATGAATATGTTCGTCAAATCCTGCCTGAAGCGAACTCGCAGATTGTTGGCT              >  SRR4302535.1346218/1‑94 (MQ=60)
                                                                                 AAAAACCGTTGAGCGTCTACGGTTACTCAAAATTCCTGTTTGATGAATATGTTCGTCAAATCCTGCCTGAAGCGAACTCGCAGATTGTTGGCTT             >  SRR4302535.3535363/1‑94 (MQ=60)
                                                                                  AAAACCGTTGAGCGTCTACGGTTACTCAAAATTCCTGTTTGATGAATATGTTCGTCAAATCCTGCCTGAAGCGAACTCGCAGATTGTTGGCTTC            <  SRR4302535.3453306/94‑1 (MQ=60)
                                                                                   AAACCGTTGAGCTTCTACGGTTACTCAAAATGCCTGTTTGATGAATATGTTCGTCAAATCCTGCCTGAAGCGAACTCGCAGATTGTTGGCTTCC           <  SRR4302535.707915/94‑1 (MQ=60)
                                                                                    AACCGTTGAGCGTCTACGGTTACTCAAAATTCCTGTTTGATGAATATGTTCGTCAAATCCTGCCTGAAGCGAACTCGCAGATTGTTGGCTTCCG          <  SRR4302535.2934606/94‑1 (MQ=60)
                                                                                    AACCGTTGAGCGTCTACGGTTACTCAAAATTCCTGTTTGATGAATATGTTCGTCAAATCCTGCCTGAAGCGAACTCGCAGATTGTTGGCTTCCG          >  SRR4302535.3264019/1‑94 (MQ=60)
                                                                                     ACCGTTGAGCGTCTACGGTTACTCAAAATTCCTGTTTGATGAATATGTTCTTCAAATCCTGCCTGAAGCGAACTCGCAGATTGTTGGCTTCCGT         <  SRR4302535.2529956/94‑1 (MQ=60)
                                                                                     ACCGTTGAGCGTCTACGGTTACTCAAAATTCCTGTTTGATGAATATGTTCGTCAAATCCTGCCTGAAGCGAACTCGCAGATTGTTGGCTTCCGT         >  SRR4302535.1069265/1‑94 (MQ=60)
                                                                                       CGTTGAGCGTCTACGGTTACTCAAAATTCCTGTTTGATGAATATGTTCGTCAAATCCTGCCTGAAGCGAACTCGCAGATTGTTGGCTTCCGTTA       <  SRR4302535.1506413/94‑1 (MQ=60)
                                                                                       CGTTGAGCGTCTACGGTTACTCAAAATTCCTGTTTGATGAATATGTTCGTCAAATCCTGCCTGAAGCGAACTCGCAGATTGTTGGCTTCCGTTA       >  SRR4302535.1935981/1‑94 (MQ=60)
                                                                                       CGTTGAGCGTCTACGGTTACTCAAAATTCCTGTTTGATGAATATGTTCGTCAAATCCTGCCTGAAGCGAACTCGCAGATTGTTGGCTTCCGTTA       >  SRR4302535.3318663/1‑94 (MQ=60)
                                                                                        GTTGAGCGTCTGCGGTTACTCAAAATTCCTGTTTGATGAATATGTTCGTCAAATCCTGCCTGAAGCGAACTCGCAGATTGTTGGCTTCCGTTAT      <  SRR4302535.660746/94‑1 (MQ=60)
                                                                                        GTTGAGCGTCTACGGTTACTCAAAATTCCTGTTTGATGAATATGTTCGTCAAATCCTGCCTGAAGCGAACTCGCAGATTGTTGGCTTCCGTTAT      <  SRR4302535.1934792/94‑1 (MQ=60)
                                                                                        GTTGAGCGTCTACGGTTACTCAAAATTCCTGTTTGATGAATATGTTCGTCAAATCCTGCCTGAAGCGAACTCGCAGATTGTTGGCTTCCGTTAT      <  SRR4302535.4502161/94‑1 (MQ=60)
                                                                                         TTGAGCGTCTACGGTTACTCAAAATTCCTGTTTGATGAATATGTTCGTCAAATCCTGCCTGAAGCGAACTCGCAGATTGTTGGCTTCCGTTATT     <  SRR4302535.4647613/94‑1 (MQ=60)
                                                                                            AGCGTCTACGGTTACTCAAAATTCCTGTTTGATGAATATGTTCGTCAAATCCTGCCTGAAGCGAACTCGCAGATTGTTGGCTTCCGTTATTTCA  >  SRR4302535.4180370/1‑94 (MQ=60)
                                                                                            AGCGTCTACGGTTACTCAAAATTCCTGTTTGATGAATATGTTCGTCAAATCCTGCCTGAAGCGAACTCGCAGATTGTTGGCTTCCGTTATTTCA  <  SRR4302535.3533960/94‑1 (MQ=60)
                                                                                             |                                                                                            
GTGAAATCCCGTTCCTGTACGCTTCTTCCGCAGCCACCTACGGCGGACGCACCTCCGACTTTATTGAATCCCGCGAGTACGAAAAACCGTTGAACGTCTACGGTTACTCAAAATTCCTGTTTGATGAATATGTTCGTCAAATCCTGCCTGAAGCGAACTCGCAGATTGTTGGCTTCCGTTATTTCA  >  NC_000913/3791933‑3792118

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑