Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 1873 183.1 9376148 98.2% 9207377 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,812,035 G→A intergenic (+21/‑86) dut → / → b3641 deoxyuridinetriphosphatase/putative transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,812,0350GA99.3% 970.3 / ‑5.5 269intergenic (+21/‑86)dut/b3641deoxyuridinetriphosphatase/putative transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base G (0/2);  new base A (118/149);  total (118/151)
Fisher's exact test for biased strand distribution p-value = 5.06e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 274 total aligned reads displayed.

TAATCTGGTGGAAGATTTCGACGCCACCGACCGCGGTGAAGGCGGCTTTGGTCACTCTGGTCGTCAGTAACACATACGCATCCGAATAACGTCATAACATAGCCGCAAACATTTCGTTTGCGGTCATAGCGTGGGTGCCGCCTGGCAAGTGCTTATTTTCAGGGGTATTTTGTAACATGG  >  NC_000913/3811945‑3812124
                                                                                          |                                                                                         
 aaTCTGGTGGAAGATTTCGACGCCACCGACCGCGGTGAAGGCGGCTTTGGTCACTCTGGTCGTCAGTAACACATACGCATCCGAATAACATCa                                                                                        <  2:2209460/93‑1 (MQ=255)
 aaTCTGGTGGAAGATTTCGACGCCACCGACCGCGGTGAAGGCGGCTTTGGTCACTCTGGTCGTCAGTAACACATACGCATCCGAATAACATCa                                                                                        >  1:2045932/1‑93 (MQ=255)
 aaTCTGGTGGAAGATTTCGACGCCACCGACCGCGGTGAAGGCGGCTTTGGTCACTCTGGTCGTCAGTAACACATACGCATCCGAATAACATCa                                                                                        <  2:4379178/93‑1 (MQ=255)
  aTCTGGTGGAAGATTTCGACGCCACCGACCGCGGTGAAGGCGGCTTTGGTCACTCTGGTCGTCAGTAACACATACGCATCCGAATAAcatcat                                                                                       <  1:1523237/93‑1 (MQ=255)
   tCTGGTGGAAGATTTCGACGCCACCGACCGCGGTGAAGGCGGCTTTGGTCACTCTGGTCGTCAGTAACACATACGCATCCGAATAACATCATa                                                                                      >  2:2740856/1‑93 (MQ=255)
       gtggAAGATTTCGACGCCACCGACCGCGGTGAAGGCGGCTTTGGTCACTCTGGTCGTCAGTAACACATACGCATCCGAATAACATCATAACAt                                                                                  <  2:1401277/93‑1 (MQ=255)
       gtggAAGATTTCGACGCCACCGACCGCGGTGAAGGCGGCTTTGGTCACTCTGGTCGTCAGTAACACATACGCATCCGAATAACATCATAACAt                                                                                  <  1:3115674/93‑1 (MQ=255)
       gtggAAGATTTCGACGCCACCGACCGCGGTGAAGGCGGCTTGGGTCACTCTGGTCGTCAGTAACACATACGCATCCGAATAACATCATAACAt                                                                                  <  1:3364617/93‑1 (MQ=255)
        tggAAGATTTCGACGCCACCGACCGCGGTGAAGGCGGCTTTGGTCACTCTGGTCGTCAGTAACACATACGCATCCGAATAACATCATAACATa                                                                                 <  2:716384/93‑1 (MQ=255)
        tggAAGATTTCGACGCCACCGACCGCGGTGAAGGCGGCTTTGGTCACTCTGGTCGTCAGTAACACATACGCATCCGAATAACATCATAACATa                                                                                 >  2:4069487/1‑93 (MQ=255)
        tggAAGATTTCGACGCCACCGACCGCGGTGAAGGCGGCTTTGGTCACTCTGGTCGTCAGTAACACATACGCATCCGAATAACATCATAACATa                                                                                 >  2:2374070/1‑93 (MQ=255)
        gggAAGATTTCGACGCCACCGACCGCGGTGAAGGCGGCTTTGGTCACTCTGGTCGTCAGTAACACATACGCATCCGAATAACATCATAACATa                                                                                 <  1:928069/92‑1 (MQ=255)
         ggAAGATTTCGACGCCACCGACCGCGGTGAAGGCGGCTTTGGTCACTCTGGTCGTCAGTAACACATACGCATCCGAATAACATCATAACATAg                                                                                >  1:3793321/1‑93 (MQ=255)
         ggAAGATTTCGACGCCACCGACCGCGGTGAAGGCGGCTTTGGTCACTCTGGTCGTCAGTAACACATACGCATCCGAATAACATCATAACATAg                                                                                >  2:1597726/1‑93 (MQ=255)
             gATTTCGACGCCACCGACCGCGGTGAAGGCGGCTTTGGTCACTCTGGTCGTCAGTAACACATACGCATCCGAATAACATCATAACATAGCCGc                                                                            <  1:1085033/93‑1 (MQ=255)
             gATTTCGACGCCACCGACCGCGGTGAAGGCGGCTTTGGTCACTCTGGTCGTCAGTAACACATACGCATCCGAATAACATCATAACATAGCCGc                                                                            >  1:2469580/1‑93 (MQ=255)
             aaTTTCGACGCCACCGACCGCGGTGAAGGCGGCTTTGGTCACTCTGGTCGTCAGTAACACATACGCATCCGAATAACATCATAACATAGCCGc                                                                            <  2:1906120/92‑1 (MQ=255)
              aTTTCGACGCCACCGACCGCGGTGAAGGCGGCTTTGGTCACTCTGGTCGTCAGTAACACATACGCATCCGAATAACATCATAACATAGCCGCa                                                                           <  1:3201222/93‑1 (MQ=255)
               tttCGACGCCACCGACCGCGGTGAAGGCGGCTTTGGTCACTCTGGTCGTCAGTAACACATACGCATCCGAATAACATCATAACATAGCCGCaa                                                                          <  2:2438187/93‑1 (MQ=255)
               tttCGACGCCACCGACCGCGGTGAAGGCGGCTTTGGTCACTCTGGTCGTCAGTAACACATACGCATCCGAATAACATCATAACATAGCCGCaa                                                                          >  1:3036669/1‑93 (MQ=255)
               tttCGACGCCACCGACCGCGGTGAAGGCGGCTTTGGTCACTCTGGTCGTCAGTAACACATACGCATCCGAATAACATCATAACATAGCCGCaa                                                                          >  2:432852/1‑93 (MQ=255)
                 tCGACGCCACCGACCGCGGTGAAGGCGGCTTTGGTCACTCTGGTCGTCAGTAACACATACGCATCCGAATAACATCATAACATAGCCGCAAAc                                                                        <  2:3608494/93‑1 (MQ=255)
                   gACGCCACCGACCGCGGTGAAGGCGGCTTTGGTCACTCTGGTCGTCAGTAACACATACGCATCCGAATAACATCATAACATAGCCGCAAACAt                                                                      >  2:3046788/1‑93 (MQ=255)
                    aCGCCACCGACCGCGGTGAAGGCGGCTTTGGTCACTCTGGTCGTCAGTAACACATACGCATCCGAATAACATCATAACATAGCCGCAAACAtt                                                                     >  1:4565995/1‑93 (MQ=255)
                    aCGCCACCGACCGCGGTGAAGGCGGCTTTGGTCACTCTGGTCGTCAGTAACACATACGCATCCGAATAACATCATAACATAGCCGCAAACAtt                                                                     <  2:4347946/93‑1 (MQ=255)
                      gCCACCGACCGCGGTGAAGGCGGCTTTGGTCACTCTGGTCGTCAGTAACACATACGCATCCGAATAACATCATAACATAGCCGCAAACATTTc                                                                   <  2:2500583/93‑1 (MQ=255)
                      gCCACCGACCGCGGTGAAGGCGGCTTTGGTCACTCTGGTCGTCAGTAACACATACGCATCCGAATAACATCATAACATAGCCGCAAACATTTc                                                                   >  2:1744843/1‑93 (MQ=255)
                      gCCACCGACCGCGGTGAAGGCGGCTTTGGTCACTCTGGTCGTCAGTAACACATACGCATCCGAATAACATCATAACATAGCCGCAAACATTTc                                                                   <  1:3684006/93‑1 (MQ=255)
                        cACCGACCGCGGTGAAGGCGGCTTTGGTCACTCTGGTCGTCAGTAACACATACGCATCCGAATAACATCATAACATAGCCGCAAACATTTCGt                                                                 <  1:2837243/93‑1 (MQ=255)
                        cACCGACCGCGGTGAAGGCGGCTTTGGTCACTCTGGTCGTCAGTAACACATACGCATCCGAATAACATCATAACATAGCAGCAAACATTTCGt                                                                 >  1:206617/1‑93 (MQ=255)
                         accgaccgCGGTGAAGGCGGCTTTGGTCACTCTGGTCGTCAGTAACACATACGCATCCGAATAACATCATAACATAGCCGCAAACATTTCGtt                                                                <  2:3102059/93‑1 (MQ=255)
                         accgaccgCGGTGAAGGCGGCTTTGGTCACTCTGGTCGTCAGTAACACATACGCATCCGAATAACATCATAACATAGCCGCAAACATTTCGtt                                                                >  2:2711939/1‑93 (MQ=255)
                         accgaccgCGGTGAAGGCGGCTTTGGTCACTCTGGTCGTCAGTAACACATACGCATCCGAATAACATCATAACATAGCCGCAAACATTTCGtt                                                                >  2:95873/1‑93 (MQ=255)
                         accgaccgCGGTGAAGGCGGCTTTGGTCACTCTGGTCGTCAGTAACACATACGCATCCGAATAACATCATAACATAGCCGCAAACATTTCGtt                                                                <  2:214436/93‑1 (MQ=255)
                          ccgaccgCGGTGAAGGCGGCTTTGGTCACTCTGGTCGTCAGTAACACATACGCATCCGAATAACATCATAACATAGCCGCAAACATTTCGttt                                                               <  2:2284785/93‑1 (MQ=255)
                          ccgaccgCGGTGAAGGCGGCTTTGGTCACTCTGGTCGTCAGTAACACATACGCATCCGAATAACATCATAACATAGCCGCAAACATTTCGttt                                                               <  2:207335/93‑1 (MQ=255)
                            gaccgCGGTGAAGGCGGCTTTGGTCACTCTGGTCGTCAGTAACACATACGCATCCGAATAACATCATAACATAGCCGCAAACATTTCGTTTGc                                                             >  2:4049066/1‑93 (MQ=255)
                            gaccgCGGTGAAGGCGGCTTTGGTCACTCTGGTCGTCAGTAACACATACGCATCCGAATAACATCATAACATAGCCGCAAACATTTCGTTTGc                                                             >  2:4630280/1‑93 (MQ=255)
                             accgCGGTGAAGGCGGCTTTGGTCACTCTGGTCGTCAGTAACACATACGCATCCGAATAACATCATAACATAGCCGCAAACATTTCGTTTGCg                                                            >  2:4226012/1‑93 (MQ=255)
                                 cgGTGAAGGCGGCTTTGGTCACTCTGGTCGTCAGTAACACATACGCATCCGAATAACATCATAACATAGCCGCAAACATTTCGTTTGCGGTCa                                                        <  2:4198441/93‑1 (MQ=255)
                                 cgGTGAAGGCGGCTTTGGTCACTCTGGTCGTCAGTAACACATACGCATCCGAATAACATCATAACATAGCCGCAAACATTTCGTTTGCGGTCa                                                        >  2:1994394/1‑93 (MQ=255)
                                   gTGAAGGCGGCTTTGGTCGCTCTGGTCGTCAGTAACACATACGCATCCGAATAACATCATAACATAGCCGCAAACATTTCGTTTGCGGTCATa                                                      <  1:1689700/93‑1 (MQ=255)
                                   gTGAAGGCGGCTTTGGTCACTCTGGTCGTCAGTAACACATACGCATCCGAATAACATCATAACATAGCCGCAAACATTTCGTTTGCGGTCATa                                                      <  1:355652/93‑1 (MQ=255)
                                   gTGAAGGCGGCTTTGGTCACTCTGGTCGTCAGTAACACATACGCATCCGAATAACATCATAACATAGCCGCAAACATTTCGTTTGCGGTCATa                                                      <  1:3458257/93‑1 (MQ=255)
                                   gTGAAGGCGACTTTGGTCACTCTGGTCGTCAGTAACACATACGCATCCGAATAACATCATAACATAGCCGCAAACATTTCGTTTGCGGTCATa                                                      >  2:1740920/1‑93 (MQ=255)
                                    tGAAGGCGGCTTTGGTCACTCTGGTCGTCAGTAACACATACGCATCCGAATAACATCATAACATAGCCGCAAACATTTCGTTTGCGGTCATAg                                                     <  2:2316772/93‑1 (MQ=255)
                                    tGAAGGCGGCTTTGGTCACTCTGGTCGTCAGTAACACATACGCATCCGAATAACATCATAACATAGCCGCAAACATTTCGTTTGCGGTCATAg                                                     <  1:1105160/93‑1 (MQ=255)
                                     gAAGGCGGCTTTGGTCACTCTGGTCGTCAGTAACACATACGCATCCGAATAACATCATAACATAGCCGCAAACATTTCGTTTGCGGTCATAGc                                                    <  1:3496141/93‑1 (MQ=255)
                                     gAAGGCGGCTTTGGTCACTCTGGTCGTCAGTAACACATACGCATCCGAATAACATCATAACATAGCCGCAAACATTTCGTTTGCGGTCATAGc                                                    <  1:2267099/93‑1 (MQ=255)
                                       aGGCGGCTTTGGTCACTCTGGTCGTCAGTAACACATACGCATCCGAATAACATCATAACATAGCCGCAAACATTTCGTTTGCGGTCATAGCGt                                                  >  2:3214865/1‑93 (MQ=255)
                                         gcggcTTTGGTCACTCTGGTCGTCAGTAACACATACGCATCCGAATAACATCATAACATAGCCGCAAACATTTCGTTTGCGGTCATAGCGTgg                                                <  1:3161142/93‑1 (MQ=255)
                                         gcggcTTTGGTCACTCTGGTCGTCAGTAACACATACGCATCCGAATAACATCATAACATAGCCGCAAACATTTCGTTTGCGGTCATAGCGTgg                                                <  1:1715601/93‑1 (MQ=255)
                                         gcggcTTTGGTCACTCTGGTCGTCAGTAACACATACGCATCCGAATAACATCATAACATAGCCGCAAACATTTCGTTTGCGGTCATAGCGTgg                                                <  1:1936602/93‑1 (MQ=255)
                                          cggcTTTGGTCACTCTGGTCGTCAGTAACACATACGCATCCGAATAACATCATAACATAGCCGCAAACATTTCGTTTGCGGTCATAGCGTggg                                               <  2:4367848/93‑1 (MQ=255)
                                            gcTTTGGTCACTCTGGTCGTCAGTAACACATACGCATCCGAATAACATCATAACATAGCCGCAAACATTTCGTTTGCGGTCATAGCGTGGGTg                                             >  1:4681184/1‑93 (MQ=255)
                                            gcTTTGGTCACTCTGGTCGTCAGTAACACATACGCATCCGAATAACATCATAACATAGCCGCAAACATTTCGTTTGCGGTCATAGCGTGGGTg                                             >  2:3560908/1‑93 (MQ=255)
                                            gcTTTGGTCACTCTGGTCGTCAGTAACACATACGCATCCGAATAACATCATAACATAGCCGCAAACATTTCGTTTGCGGTCATAGCGTGGGTg                                             >  2:3843805/1‑93 (MQ=255)
                                             cTTTGGTCACTCTGGTCGTCAGTAACACATACGCATCCGAATAACATCATAACATAGCCGCAAACATTTCGTTTGCGGTCATAGCGTGGGTgc                                            >  1:1319772/1‑93 (MQ=255)
                                             cTTTGGTCACTCTGGTCGTCAGTAACACATACGCATCCGAATAACATCATAACATAGCCGCAAACATTTCGTTTGCGGTCATAGCGTGGGTgc                                            >  2:3254763/1‑93 (MQ=255)
                                              tttGGTCACTCTGGTCGTCAGTAACACATACGCATCCGAATAACATCATAACATAGCCGCAAACATTTCGTTTGCGGTCATAGCGTGGGTgcc                                           <  2:1082607/93‑1 (MQ=255)
                                               ttGGTCACTCTGGTCGTCAGTAACACATACGCATCCGAATAACATCATAACATAGCCGCAAACATTTCGTTTGCGGTCATAGCGTGGGTgccg                                          >  1:2937320/1‑93 (MQ=255)
                                                 ggTCACTCTGGTCGTCAGTAACACATACGCATCCGAATAACATCATAACATAGCCGCAAACATTTCGTTTGCGGTCATAGCGTGGGTgccgcc                                        <  1:3155247/93‑1 (MQ=255)
                                                   tCACTCTGGTCGTCAGTAACACATACGCATCCGAATAACATCATAACATAGCCGCAAACATTTCGTTTGCGGTCATAGCGTGGGTGCCGCCTg                                      <  1:4484332/93‑1 (MQ=255)
                                                     aCTCTGGTCGTCAGTAACACATACGCATCCGAATAACATCATAACATAGCCGCAAACATTTCGTTTGCGGTCATAGCGTGGGTGCCGCCTGGc                                    >  1:1466394/1‑93 (MQ=255)
                                                        ctGGTCGTCAGTAACACATACGCATCCGAATAACATCATAACATAGCCGCAAACATTTCGTTTGCGGTCATAGCGTGGGTGCCGCCTGGCAAg                                 <  2:1946157/93‑1 (MQ=255)
                                                               tcAGTAACACATACGCATCCGAATAACATCATAACATAGCCGCAAACATTTCGTTTGCGGTCATAGCGTGGGTGCCGCCTGGCAAGTGCTTAt                          <  1:4015938/93‑1 (MQ=255)
                                                               tcAGTAACACATACGCATCCGAATAACATCATAACATAGCCGCAAACATTTCGTTTGCGGTCATAGCGTGGGTGCCGCCTGGCAAGTGCTTAt                          >  1:792305/1‑93 (MQ=255)
                                                               tcAGTAACACATACGCATCCGAATAACATCATAACATAGCCGCAAACATTTCGTTTGCGGTCATAGCGTGGGTGCCGCCTGGCAAGTGCTTAt                          >  2:3249631/1‑93 (MQ=255)
                                                               tcAGTAACACATACGCATCCGAATAACATCATAACATAGCCGCAAACATTTCGTTTGCGGTCATAGCGTGGGTGCCGCCTGGCAAGTGCTTAt                          <  2:2613613/93‑1 (MQ=255)
                                                                cAGTAACACATACGCATCCGAATAACATCATAACATAGCCGCAAACATTTCGTTTGCGGTCATAGCGTGGGTGCCGCCTGGCAAGTGCTTAtt                         <  1:411378/93‑1 (MQ=255)
                                                                cAGTAACACATACGCATCCGAATAACATCATAACATAGCCGCAAACATTTCGTTTGCGGTCATAGCGTGGGTGCCGCCTGGCAAGTGCTTAtt                         <  1:1366433/93‑1 (MQ=255)
                                                                cAGTAACACATACGCATCCGAATAACATCATAACATAGCCGCAAACATTTCGTTTGCGGTCATAGCGTGGGTGCCGCCTGGCAAGTGCTTAtt                         <  2:3140524/93‑1 (MQ=255)
                                                                  gTAACACATACGCATCCGAATAACATCATAACATAGCCGCAAACATTTCGTTTGCGGTCATAGCGTGGGTGCCGCCTGGCAAGTGCTTAtttt                       >  2:1016218/1‑93 (MQ=255)
                                                                    aaCACATACGCATCCGAATAACATCATAACATAGCCGCAAACATTTCGTTTGCGGTCATAGCGTGGGTGCCGCCTGGCAAGTGCTTATTTTCa                     <  1:347161/93‑1 (MQ=255)
                                                                       acaTACGCATCCGAATAACATCATAACATAGCCGCAAACATTTCGTTTGCGGTCATAGCGTGGGTGCCGCCTGGCAAGTGCTTATTTTCAggg                  >  1:1418066/1‑93 (MQ=255)
                                                                       acaTACGCATCCGAATAACATCATAACATAGCCGCAAACATTTCGTTTGCGGTCATAGCGTGGGTGCCGCCTGGCAAGTGCTTATTTTCAggg                  >  1:3317424/1‑93 (MQ=255)
                                                                        caTACGCATCCGAATAACATCATAACATAGCCGCAAACATTTCGTTTGCGGTCATAGCGTGGGTGCCGCCTGGCAAGTGCTTATTTTCAgggg                 <  2:1453373/93‑1 (MQ=255)
                                                                        caTACGCATCCGAATAACATCATAACATAGCCGCAAACATTTCGTTTGCGGTCATAGCGTGGGTGCCGCCTGGCAAGTGCTTATTTTCAgggg                 <  2:514556/93‑1 (MQ=255)
                                                                         aTACGCATCCGAATAACATCATAACATAGCCGCAAACATTTCGTTTGCGGTCATAGCGTGGGTGCCGCCTGGCAAGTGCTTATTTTCAGGGGt                <  1:71029/93‑1 (MQ=255)
                                                                         aTACGCATCCGAATAACATCATAACATAGCCGCAAACATTTCGTTTGCGGTCATAGCGTGGGTGCCGCCTGGCAAGTGCTTATTTTCAGGGGt                >  1:21182/1‑93 (MQ=255)
                                                                           aCGCATCCGAATAACGTCATAACATAGCCGCAAACATTTCGTTTGCGGTCATAGCGTGGGTGCCGCCTGGCAAGTGCTTATTTTCAGGGGTAt              <  1:230899/93‑1 (MQ=255)
                                                                           aCGCATCCGAATAACATCATAACATAGCCGCAAACATTTCGTTTGCGGTCATAGCGTGGGTGCCGCCTGGCAAGTGCTTATTTTCAGGGGTAt              <  2:2567166/93‑1 (MQ=255)
                                                                           aCGCATCCGAATAACATCATAACATAGCCGCAAACATTTCGTTTGCGGTCATAGCGTGGGTGCCGCCTGGCAAGTGCTTATTTTCAGGGGTAt              >  2:3271110/1‑93 (MQ=255)
                                                                           aCGCATCCGAATAACATCATAACATAGCCGCAAACATTTCGTTTGCGGTCATAGCGTGGGTGCCGCCTGGCAAGTGCTTATTTTCAGGGGTAt              <  1:406464/93‑1 (MQ=255)
                                                                            cGCATCCGAATAACATCATAACATAGCCGCAAACATTTCGTTTGCGGTCATAGCGTGGGTGCCGCCTGGCAAGTGCTTATTTTCAGGGGTAtt             <  2:1052569/93‑1 (MQ=255)
                                                                              cATCCGAATAACATCATAACATAGCCGCAAACATTTCGTTTGCGGTCATAGCGTGGGTGCCGCCTGGCAAGTGCTTATTTTCAGGGGTAtttt           >  1:2316998/1‑93 (MQ=255)
                                                                               aTCCGAATAACATCATAACATAGCCGCAAACATTTCGTTTGCGGTCATAGCGTGGGTGCCGCCTGGCAAGTGCTTATTTTCAGGGGTATTTTg          >  1:1626478/1‑93 (MQ=255)
                                                                                tCCGAATAACATCATAACATAGCCGCAAACATTTCGTTTGCGGTCATAGCGTGGGTGCCGCCTGGCAAGTGCTTATTTTCAGGGGTATTTTGt         <  2:3415043/93‑1 (MQ=255)
                                                                                 ccGAATAACATCATAACATAGCCGCAAACATTTCGTTTGCGGTCATAGCGTGGGTGCCGCCTGGCAAGTGCTTATTTTCAGGGGTATTTTGTa        <  2:2823758/93‑1 (MQ=255)
                                                                                  cGAATAACATCATAACATAGCCGCAAACATTTCGTTTGCGGTCATAGCGTGGGTGCCGCCTGGCAAGTGCTTATTTTCAGGGGTATTTTGTaa       >  2:4417696/1‑93 (MQ=255)
                                                                                  cGAATAACATCATAACATAGCCGCAAACATTTCGTTTGCGGTCATAGCGTGGGTGCCGCCTGGCAAGTGCTTATTTTCAGGGGTATTTTGTaa       >  2:1686200/1‑93 (MQ=255)
                                                                                   gAATAACATCATAACATAGCCGCAAACATTTCGTTTGCGGTCATAGCGTGGGTGCCGCCTGGCAAGTGCTTATTTTCAGGGGTATTTTGTAAc      >  2:673516/1‑93 (MQ=255)
                                                                                   gAATAACATCATAACATAGCCGCAAACATTTCGTTTGCGGTCATAGCGTGGGTGCCGCCTGGCAAGTGCTTATTTTCAGGGGTATTTTGTAAc      >  2:3883415/1‑93 (MQ=255)
                                                                                   gAATAACATCATAACATAGCCGCAAACATTTCGTTTGCGGTCATAGCGTGGGTGCCGCCTGGCAAGTGCTTATTTTCAGGGGTATTTTGTAAc      <  2:2275491/93‑1 (MQ=255)
                                                                                    aaTAACATCATAACATAGCCGCAAACATTTCGTTTGCGGTCATAGCGTGGGTGCCGCCTGGCAAGTGCTTATTTTCAGGGGTATTTTGTAACa     >  1:264754/1‑93 (MQ=255)
                                                                                    aaTAACATCATAACATAGCCGCAAACATTTCGTTTGCGGTCATAGCGTGGGTGCCGCCTGGCAAGTGCTTATTTTCAGGGGTATTTTGTAACa     <  2:4555554/93‑1 (MQ=255)
                                                                                     aTAACATCATAACATAGCCGCAAACATTTCGTTTGCGGTCATAGCGTGGGTGCCGCCTGGCAAGTGCTTATTTTCAGGGGTATTTTGTAACAt    <  1:4343145/93‑1 (MQ=255)
                                                                                      tAACATCATAACATAGCCGCAAACATTTCGTTTGCGGTCATAGCGTGGGTGCCGCCTGGCAAGTGCTTATTTTCAGGGGTATTTTGTAACATg   <  1:4542021/93‑1 (MQ=255)
                                                                                      tAACATCATAACATAGCCGCAAACATTTCGTTTGCGGTCATAGCGTGGGTGCCGCCTGGCAAGTGCTTATTTTCAGGGGTATTTTGTAACATg   >  2:897724/1‑93 (MQ=255)
                                                                                       aaCATCATAACATAGCCGCAAACATTTCGTTTGCGGTCATAGCGTGGGTGCCGCCTGGCAAGTGCTTATTTTCAGGGGTATTTTGTAACATgg  >  2:1311449/1‑93 (MQ=255)
                                                                                          |                                                                                         
TAATCTGGTGGAAGATTTCGACGCCACCGACCGCGGTGAAGGCGGCTTTGGTCACTCTGGTCGTCAGTAACACATACGCATCCGAATAACGTCATAACATAGCCGCAAACATTTCGTTTGCGGTCATAGCGTGGGTGCCGCCTGGCAAGTGCTTATTTTCAGGGGTATTTTGTAACATGG  >  NC_000913/3811945‑3812124

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
ATTTAATCTGGTGGAAGATTTCGACGCCACCGACCGCGGTGAAGGCGGCTTTGGTCACTCTGGTCGTCAGTAACACATACGCATCCGAATAACGTCATAACATAGCCGCAAACATTTCGTTTGCGGTCATAGCGTGGGTGCCGCCTGGCAAGTGCTTATTTTCAGGGGTATTTTGTAACATGGCAGA  >  NC_000913/3811942‑3812128
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ATTTAATCTGGTGGAAGATTTCGACGCCACCGACCGCGGTGAAGGCGGCTTTGGTCACTCTGGTCGTCAGTAACACATACGCATCCGAATAACA                                                                                               <  SRR4302535.1156410/94‑1 (MQ=60)
  TTAATCTGGTGGAAGATTTCGACGCCACCGACCGCGGTGAAGGCGGCTTTGGTCACTCTGGTCGTCAGTAACACATACGCATCCGAATAACATC                                                                                             <  SRR4302535.3572362/94‑1 (MQ=60)
   TAATCTGGTGGAAGATTTCGACGCCACCGACCGCGGTGAAGGCGGCTTTGGTCACTCTGGTCGTCAGTAACACATACGCATCCGAATAACATCA                                                                                            >  SRR4302535.4138346/1‑94 (MQ=60)
   TAATCTGGTGGAAGATTTCGACGCCACCGACCGCGGTGAAGGCGGCTTTGGTCACTCTGGTCGTCAGTAACACATACGCATCCGAATAACATCA                                                                                            >  SRR4302535.2060667/1‑94 (MQ=60)
    AATCTGGTGGAAGATTTCGACGCCACCGACCGCGGTGAAGGCGGCTTTGGTCACTCTGGTCGTCAGTAACACATACGCATCCGAATAACATCAT                                                                                           <  SRR4302535.938557/94‑1 (MQ=60)
    AATCTGGTGGAAGATTTCGACGCCACCGACCGCGGTGAAGGCGGCTTTGGTCACTCTGGTCGTCAGTAACACATACGCATCCGAATAACATCAT                                                                                           >  SRR4302535.1293338/1‑94 (MQ=60)
     ATCTGGTGGAAGATTTCGACGCCACCGACCGCGGTGAAGGCGGCTTTGGTCACTCTGGTCGTCAGTAACACATACGCATCCGAATAACATCATA                                                                                          <  SRR4302535.467437/94‑1 (MQ=60)
     ATCTGGTGGAAGATTTCGACGCCACCGACCGCGGTGAAGGCGGCTTTGGTCACTCTGGTCGTCAGTAACACATACGCATCCGAATAACATCATA                                                                                          <  SRR4302535.1534208/94‑1 (MQ=60)
      TCTGGTGGAAGATTTCGACGCCACCGACCGCGGTGAAGGCGGCTTTGGTCACTCTGGTCGTCAGTAACACATACGCATCCGAATAACATCATAA                                                                                         <  SRR4302535.3676511/94‑1 (MQ=60)
        TGGTGGAAGATTTCGACGCCACCGACCGCGGTGAAGGCGGCTTTGGTCACTCTGGTCGTCAGTAACACATACGCATCCGAATAACATCATAACA                                                                                       <  SRR4302535.351225/94‑1 (MQ=60)
          GTGGAAGATTTCGACGCCACCGACCGCGGTGAAGGCGGCTTTGGTCACTCTGGTCGTCAGTAACACATACGCATCCGAATAACATCATAACATA                                                                                     <  SRR4302535.3137366/94‑1 (MQ=60)
          GTGGAAGATTTCGACGCCACCGACCGCGGTGAAGGCGGCTTTGGTCACTCTGGTCGTCAGTAACACATACGCATCCGAATAACATCATAACATA                                                                                     >  SRR4302535.2315808/1‑94 (MQ=60)
          GTGGAAGATTTCGACGCCACCGACCGCGGTGAAGGCGGCTTGGGTCACTCTGGTCGTCAGTAACACATACGCATCCGAATAACATCATAACATA                                                                                     <  SRR4302535.3388028/94‑1 (MQ=60)
           TGGAAGATTTCGACGCCACCGACCGCGGTGAAGGCGGCTTTGGTCACTCTGGTCGTCAGTAACACATACGCATCCGAATAACATCATAACATAG                                                                                    >  SRR4302535.3819475/1‑94 (MQ=60)
           GGGAAGATTTCGACGCCACCGACCGCGGTGAAGGCGGCTTTGGTCACTCTGGTCGTCAGTAACACATACGCATCCGAATAACATCATAACATAG                                                                                    <  SRR4302535.934641/94‑1 (MQ=60)
             GAAGATTTCGACGCCACCGACCGCGGTGAAGGCGGCTTTGGTCACTCTGGTCGTCAGTAACACATACGCATCCGAATAACATCATAACATAGCC                                                                                  <  SRR4302535.3144191/94‑1 (MQ=60)
              AAGATTTCGACGCCACCGACCGCGGTGAAGGCGGCTTTGGTCACTCTGGTCGTCAGTAACACATACGCATCCGAATAACATCATAACATAGCCG                                                                                 <  SRR4302535.625999/94‑1 (MQ=60)
               AGATTTCGACGCCACCGACCGCGGTGAAGGCGGCTTTGGTCACTCTGGTCGTCAGTAACACATACGCATCCGAATAACATCATAACATAGCCGC                                                                                >  SRR4302535.2487383/1‑94 (MQ=60)
               AGATTTCGACGCCACCGACCGCGGTGAAGGCGGCTTTGGTCACTCTGGTCGTCAGTAACACATACGCATCCGAATAACATCATAACATAGCCGC                                                                                <  SRR4302535.3489013/94‑1 (MQ=60)
               AGATTTCGACGCCACCGACCGCGGTGAAGGCGGCTTTGGTCACTCTGGTCGTCAGTAACACATACGCATCCGAATAACATCATAACATAGCCGC                                                                                >  SRR4302535.854021/1‑94 (MQ=60)
                GATTTCGACGCCACCGACCGCGGTGAAGGCGGCTTTGGTCACTCTGGTCGTCAGTAACACATACGCATCCGAATAACATCATAACATAGCCGCA                                                                               <  SRR4302535.1092747/94‑1 (MQ=60)
                 ATTTCGACGCCACCGACCGCGGTGAAGGCGGCTTTGGTCACTCTGGTCGTCAGTAACACATACGCATCCGAATAACATCATAACATAGCCGCAA                                                                              >  SRR4302535.1593995/1‑94 (MQ=60)
                 ATTTCGACGCCACCGACCGCGGTGAAGGCGGCTTTGGTCACTCTGGTCGTCAGTAACACATACGCATCCGAATAACATCATAACATAGCCGCAA                                                                              >  SRR4302535.3057937/1‑94 (MQ=60)
                 ATTTCGACGCCACCGACCGCGGTGAAGGCGGCTTTGGTCACTCTGGTCGTCAGTAACACATACGCATCCGAATAACATCATAACATAGCCGCAA                                                                              <  SRR4302535.4490656/94‑1 (MQ=60)
                 ATTTCGACGCCACCGACCGCGGTGAAGGCGGCTTTGGTCACTCTGGTCGTCAGTAACACATACGCATCCGAATAACATCATAACATAGCCGCAA                                                                              <  SRR4302535.3223388/94‑1 (MQ=60)
                 ATTTCGACGCCACCGACCGCGGTGAAGGCGGCTTTGGTCACTCTGGTCGTCAGTAACACATACGCATCCGAATAACATCATAACATAGCCGCAA                                                                              >  SRR4302535.3353046/1‑94 (MQ=60)
                 ATTTCGACGCCACCGACCGCGGTGAAGGCGGCTTTGGTCACTCTGGTCGTCAGTAACACATACGCATCCGAATAACATCATAACATAGCCGCAA                                                                              <  SRR4302535.353920/94‑1 (MQ=60)
                  TTTCGACGCCACCGACCGCGGTGAAGGCGGCTTTGGTCACTCTGGTCGTCAGTAACACATACGCATCCGAATAACATCATAACATAGCCGCAAA                                                                             <  SRR4302535.3467373/94‑1 (MQ=60)
                   TTCGACGCCACCGACCGCGGTGAAGGCGGCTTTGGTCACTCTGGTCGTCAGTAACACATACGCATCCGAATAACATCATAACATAGCCGCAAAC                                                                            >  SRR4302535.2312301/1‑94 (MQ=60)
                    TCGACGCCACCGACCGCGGTGAAGGCGGCTTTGGTCACTCTGGTCGTCAGTAACACATACGCATCCGAATAACATCATAACATAGCCGCAAACA                                                                           <  SRR4302535.1112354/94‑1 (MQ=60)
                     CGACGCCACCGACCGCGGTGAAGGCGGCTTTGGTCACTCTGGTCGTCAGTAACACATACGCATCCGAATAACATCATAACATAGCCGCAAACAT                                                                          <  SRR4302535.1704257/94‑1 (MQ=60)
                      GACGCCACCGACCGCGGTGAAGGCGGCTTTGGTCACTCTGGTCGTCAGTAACACATACGCATCCGAATAACATCATAACATAGCCGCAAACATT                                                                         >  SRR4302535.4597144/1‑94 (MQ=60)
                       ACGCCACCGACCGCGGTGAAGGCGGCTTTGGTCACTCTGGTCGTCAGTAACACATACGCATCCGAATAACATCATAACATAGCCGCAAACATTT                                                                        <  SRR4302535.3318004/94‑1 (MQ=60)
                        CGCCACCGACCGCGGTGAAGGCGGCTTTGGTCACTCTGGTCGTCAGTAACACATACGCATCCGAATAACATCATAACATAGCCGCAAACATTTC                                                                       <  SRR4302535.511387/94‑1 (MQ=60)
                        CGCCACCGACCGCGGTGAAGGCGGCTTTGGTCACTCTGGTCGTCAGTAACACATACGCATCCGAATAACATCATAACATAGCCGCAAACATTTC                                                                       >  SRR4302535.417175/1‑94 (MQ=60)
                         GCCACCGACCGCGGTGAAGGCGGCTTTGGTCACTCTGGTCGTCAGTAACACATACGCATCCGAATAACATCATAACATAGCCGCAAACATTTCG                                                                      <  SRR4302535.3709497/94‑1 (MQ=60)
                         GCCACCGACCGCGGTGAAGGCGGCTTTGGTCACTCTGGTCGTCAGTAACACATACGCATCCGAATAACATCATAACATAGCCGCAAACATTTCG                                                                      >  SRR4302535.3712502/1‑94 (MQ=60)
                          CCACCGACCGCGGTGAAGGCGGCTTTGGTCACTCTGGTCGTCAGTAACACATACGCATCCGAATAACATCATAACATAGCCGCAAACATTTCGT                                                                     <  SRR4302535.148993/94‑1 (MQ=60)
                          CCACCGACCGCGGTGAAGGCGGCTTTGGTCACTCTGGTCGTCAGTAACACATACGCATCCGAATAACATCATAACATAGCAGCAAACATTTCGT                                                                     >  SRR4302535.208035/1‑94 (MQ=60)
                           CACCGACCGCGGTGAAGGCGGCTTTGGTCACTCTGGTCGTCAGTAACACATACGCATCCGAATAACATCATAACATAGCCGCAAACATTTCGTT                                                                    >  SRR4302535.1776824/1‑94 (MQ=60)
                           CACCGACCGCGGTGAAGGCGGCTTTGGTCACTCTGGTCGTCAGTAACACATACGCATCCGAATAACATCATAACATAGCCGCAAACATTTCGTT                                                                    >  SRR4302535.165724/1‑94 (MQ=60)
                           CACCGACCGCGGTGAAGGCGGCTTTGGTCACTCTGGTCGTCAGTAACACATACGCATCCGAATAACATCATAACATAGCCGCAAACATTTCGTT                                                                    <  SRR4302535.2857377/94‑1 (MQ=60)
                            ACCGACCGCGGTGAAGGCGGCTTTGGTCACTCTGGTCGTCAGTAACACATACGCATCCGAATAACATCATAACATAGCCGCAAACATTTCGTTT                                                                   <  SRR4302535.1957417/94‑1 (MQ=60)
                             CCGACCGCGGTGAAGGCGGCTTTGGTCACTCTGGTCGTCAGTAACACATACGCATCCGAATAACATCATAACATAGCCGCAAACATTTCGTTTG                                                                  <  SRR4302535.1757704/94‑1 (MQ=60)
                              CGACCGCGGTGAAGGCGGCTTTGGTCACTCTGGTCGTCAGTAACACATACGCATCCGAATAACATCATAACATAGCCGCAAACATTTCGTTTGC                                                                 <  SRR4302535.7360/94‑1 (MQ=60)
                              CGACCGCGGTGAAGGCGGCTTTGGTCACTCTGGTCGTCAGTAACACATACGCATCCGAATAACATCATAACATAGCCGCAAACATTTCGTTTGC                                                                 >  SRR4302535.2143576/1‑94 (MQ=60)
                              CGACCGCGGTGAAGGCGGCTTTGGTCACTCTGGTCGTCAGTAACACATACGCATCCGAATAACATCATAACATAGCCGCAAACATTTCGTTTGC                                                                 >  SRR4302535.4573928/1‑94 (MQ=60)
                                  CGCGGTGAAGGCGGCTTTGGTCACTCTGGTCGTCAGTAACACATACGCATCCGAATAACATCATAACATAGCCGCAAACATTTCGTTTGCGGTC                                                             <  SRR4302535.3618301/94‑1 (MQ=60)
                                  CGCGGTGAAGGCGGCTTTGGTCACTCTGGTCGTCAGTAACACATACGCATCCGAATAACATCATAACATAGCCGCAAACATTTCGTTTGCGGTC                                                             >  SRR4302535.3250625/1‑94 (MQ=60)
                                   GCGGTGAAGGCGGCTTTGGTCACTCTGGTCGTCAGTAACACATACGCATCCGAATAACATCATAACATAGCCGCAAACATTTCGTTTGCGGTCA                                                            >  SRR4302535.1348129/1‑94 (MQ=60)
                                    CGGTGAAGGCGGCTTTGGTCACTCTGGTCGTCAGTAACACATACGCATCCGAATAACATCATAACATAGCCGCAAACATTTCGTTTGCGGTCAT                                                           >  SRR4302535.3804769/1‑94 (MQ=60)
                                    CGGTGAAGGCGGCTTTGGTCACTCTGGTCGTCAGTAACACATACGCATCCGAATAACATCATAACATAGCCGCAAACATTTCGTTTGCGGTCAT                                                           >  SRR4302535.2468196/1‑94 (MQ=60)
                                    CGGTGAAGGCGGCTTTGGTCACTCTGGTCGTCAGTAACACATACGCATCCGAATAACATCATAACATAGCCGCAAACATTTCGTTTGCGGTCAT                                                           <  SRR4302535.2667753/94‑1 (MQ=60)
                                     GGTGAAGGCGGCTTTGGTCACTCTGGTCGTCAGTAACACATACGCATCCGAATAACATCATAACATAGCCGCAAACATTTCGTTTGCGGTCATA                                                          >  SRR4302535.2332480/1‑94 (MQ=60)
                                      GTGAAGGCGGCTTTGGTCGCTCTGGTCGTCAGTAACACATACGCATCCGAATAACATCATAACATAGCCGCAAACATTTCGTTTGCGGTCATAG                                                         <  SRR4302535.1701930/94‑1 (MQ=60)
                                      GTGAAGGCGGCTTTGGTCACTCTGGTCGTCAGTAACACATACGCATCCGAATAACATCATAACATAGCCGCAAACATTTCGTTTGCGGTCATAG                                                         <  SRR4302535.3482302/94‑1 (MQ=60)
                                      GTGAAGGCGGCTTTGGTCACTCTGGTCGTCAGTAACACATACGCATCCGAATAACATCATAACATAGCCGCAAACATTTCGTTTGCGGTCATAG                                                         <  SRR4302535.2119973/94‑1 (MQ=60)
                                      GTGAAGGCGGCTTTGGTCACTCTGGTCGTCAGTAACACATACGCATCCGAATAACATCATAACATAGCCGCAAACATTTCGTTTGCGGTCATAG                                                         <  SRR4302535.358104/94‑1 (MQ=60)
                                       TGAAGGCGGCTTTGGTCACTCTGGTCGTCAGTAACACATACGCATCCGAATAACATCATAACATAGCCGCAAACATTTCGTTTGCGGTCATAGC                                                        <  SRR4302535.1113056/94‑1 (MQ=60)
                                        cgctcttccgatctgcgtggTGGTCGTCAGTAACACATACGCATCCGAATAACATCATAACATAGCCGCAAACATTTCGTTTGCGGTCATAGCG                                                       <  SRR4302535.2651180/74‑1 (MQ=60)
                                        GAAGGCGGCTTTGGTCACTCTGGTCGTCAGTAACACATACGCATCCGAATAACATCATAACATAGCCGCAAACATTTCGTTTGCGGTCATAGCG                                                       <  SRR4302535.3520438/94‑1 (MQ=60)
                                        GAAGGCGGCTTTGGTCACTCTGGTCGTCAGTAACACATACGCATCCGAATAACATCATAACATAGCCGCAAACATTTCGTTTGCGGTCATAGCG                                                       >  SRR4302535.4238521/1‑94 (MQ=60)
                                        GAAGGCGGCTTTGGTCACTCTGGTCGTCAGTAACACATACGCATCCGAATAACATCATAACATAGCCGCAAACATTTCGTTTGCGGTCATAGCG                                                       <  SRR4302535.2283259/94‑1 (MQ=60)
                                          AGGCGGCTTTGGTCACTCTGGTCGTCAGTAACACATACGCATCCGAATAACATCATAACATAGCCGCAAACATTTCGTTTGCGGTCATAGCGTG                                                     >  SRR4302535.3006696/1‑94 (MQ=60)
                                            GCGGCTTTGGTCACTCTGGTCGTCAGTAACACATACGCATCCGAATAACATCATAACATAGCCGCAAACATTTCGTTTGCGGTCATAGCGTGGG                                                   <  SRR4302535.3183071/94‑1 (MQ=60)
                                            GCGGCTTTGGTCACTCTGGTCGTCAGTAACACATACGCATCCGAATAACATCATAACATAGCCGCAAACATTTCGTTTGCGGTCATAGCGTGGG                                                   <  SRR4302535.1950548/94‑1 (MQ=60)
                                            GCGGCTTTGGTCACTCTGGTCGTCAGTAACACATACGCATCCGAATAACATCATAACATAGCCGCAAACATTTCGTTTGCGGTCATAGCGTGGG                                                   >  SRR4302535.4440600/1‑94 (MQ=60)
                                            GCGGCTTTGGTCACTCTGGTCGTCAGTAACACATACGCATCCGAATAACATCATAACATAGCCGCAAACATTTCGTTTGCGGTCATAGCGTGGG                                                   <  SRR4302535.1728011/94‑1 (MQ=60)
                                              GGCTTTGGTCACTCTGGTCGTCAGTAACACATACGCATCCGAATAACATCATAACATAGCCGCAAACATTTCGTTTGCGGTCATAGCGTGGGTG                                                 >  SRR4302535.2679568/1‑94 (MQ=60)
                                              GGCTTTGGTCACTCTGGTCGTCAGTAACACATACGCATCCGAATAACATCATAACATAGCCGCAAACATTTCGTTTGCGGTCATAGCGTGGGTG                                                 >  SRR4302535.4712812/1‑94 (MQ=60)
                                               GCTTTGGTCACTCTGGTCGTCAGTAACACATACGCATCCGAATAACATCATAACATAGCCGCAAACATTTCGTTTGCGGTCATAGCGTGGGTGC                                                >  SRR4302535.1329202/1‑94 (MQ=60)
                                                 TTTGGTCACTCTGGTCGTCAGTAACACATACGCATCCGAATAACATCATAACATAGCCGCAAACATTTCGTTTGCGGTCATAGCGTGGGTGCCG                                              >  SRR4302535.2957984/1‑94 (MQ=60)
                                                 TGTGGTCACTCTGGTCGTCAGTAACACATACGCATCCGAATACCATCATAACATAGCCGCAAACTTTTCGTTTGCGGTCATAGCGTGGGTGCCG                                              <  SRR4302535.3487088/94‑1 (MQ=60)
                                                    GGTCACTCTGGTCGTCAGTAACACATACGCATCCGAATAACATCATAACATAGCCGCAAACATTTCGTTTGCGGTCATAGCGTGGGTGCCGCCT                                           <  SRR4302535.3177163/94‑1 (MQ=60)
                                                    GGTCACTCTGGTCGTCAGTAACACATACGCATCCGAATAACATCATAACATAGCCGCAAACATTTCGTTTGCGGTCATAGCGTGGGTGCCGCCT                                           <  SRR4302535.2642261/94‑1 (MQ=60)
                                                      TCACTCTGGTCGTCAGTAACACATACGCATCCGAATAACATCATAACATAGCCGCAAACATTTCGTTTGCGGTCATAGCGTGGGTGCCGCCTGG                                         <  SRR4302535.4515117/94‑1 (MQ=60)
                                                       CACTCTGGTCGTCAGTAACACATACGCATCCGAATAACATCATAACATAGCCGCAAACATTTCGTTTGCGGTCATAGCGTGGGTGCCGCCTGGC                                        >  SRR4302535.1476900/1‑94 (MQ=60)
                                                       CACTCTGGTCGTCAGTAACACATACGCATCCGAATAACATCATAACATAGCCGCAAACATTTCGTTTGCGGTCATAGCGTGGGTGCCGCCTGGC                                        >  SRR4302535.1551939/1‑94 (MQ=60)
                                                        ACTCTGGTCGTCAGTAACACATACGCATCCGAATAACATCATAACATAGCCGCAAACATTTCGTTTGCGGTCATAGCGTGGGTGCCGCCTGGCA                                       >  SRR4302535.3457944/1‑94 (MQ=60)
                                                           CTGGTCGTCAGTAACACATACGCATCCGAATAACATCATAACATAGCCGCAAACATTTCGTTTGCGGTCATAGCGTGGGTGCCGCCTGGCAAGT                                    >  SRR4302535.1309319/1‑94 (MQ=60)
                                                           CTGGTCGTCAGTAACACATACGCATCCGAATAACATCATAACATAGCCGCAAACATTTCGTTTGCGGTCATAGCGTGGGTGCCGCCTGGCAAGT                                    >  SRR4302535.3177573/1‑94 (MQ=60)
                                                           CTGGTCGTCAGTAACACATACGCATCCGAATAACATCATAACATAGCCGCAAACATTTCGTTTGCGGTCATAGCGTGGGTGCCGCCTGGCAAGT                                    <  SRR4302535.1432242/94‑1 (MQ=60)
                                                            TGGTCGTCAGTAACACATACGCATCCGAATAACATCATAACATAGCCGCAAACATTTCGTTTGCGGTCATAGCGTGGGTGCCGCCTGGCAAGTG                                   <  SRR4302535.4050121/94‑1 (MQ=60)
                                                              GTCGTCAGTAACACATACGCATCCGAATAACATCATAACATAGCCGCAAACATTTCGTTTGCGGTCATAGCGTGGGTGCCGCCTGGCAAGTGCT                                 <  SRR4302535.2871546/94‑1 (MQ=60)
                                                               TCGTCAGTAACACATACGCATCCGAATAACATCATAACATAGCCGCAAACATTTCGTTTGCGGTCATAGCGTGGGTGCCGCCTGGCAAGTGCTT                                <  SRR4302535.2102481/94‑1 (MQ=60)
                                                                 GTCAGTAACACATACGCATCCGAATAACATCATAACATAGCCGCAAACATTTCGTTTGCGGTCATAGCGTGGGTGCCGCCTGGCAAGTGCTTAT                              >  SRR4302535.797872/1‑94 (MQ=60)
                                                                  TCAGTAACACATACGCATCCGAATAACATCATAACATAGCCGCAAACATTTCGTTTGCGGTCATAGCGTGGGTGCCGCCTGGCAAGTGCTTATT                             <  SRR4302535.1474639/94‑1 (MQ=60)
                                                                  TCAGTAACACATACGCATCCGAATAACATCATAACATAGCCGCAAACATTTCGTTTGCGGTCATAGCGTGGGTGCCGCCTGGCAAGTGCTTATT                             <  SRR4302535.4043406/94‑1 (MQ=60)
                                                                   CAGTAACACATACGCATCCGAATAACATCATAACATAGCCGCAAACATTTCGTTTGCGGTCATAGCGTGGGTGCCGCCTGGCAAGTGCTTATTT                            <  SRR4302535.414268/94‑1 (MQ=60)
                                                                   CAGTAACACATACGCATCCGAATAACATCATAACATAGCCGCAAACATTTCGTTTGCGGTCATAGCGTGGGTGCCGCCTGGCAAGTGCTTATTT                            <  SRR4302535.1376201/94‑1 (MQ=60)
                                                                     GTAACACATACGCATCCGAATAACATCATAACATAGCCGCAAACATTTCGTTTGCGGTCATAGCGTGGGTGCCGCCTGGCAAGTGCTTATTTTC                          <  SRR4302535.457917/94‑1 (MQ=60)
                                                                      TAACACATACGCATCCGAATAACATCATAACATAGCCGCAAACATTTCGTTTGCGGTCATAGCGTGGGTGCCGCCTGGCAAGTGCTTATTTTCA                         <  SRR4302535.1490119/94‑1 (MQ=60)
                                                                      TAACACATACGCATCCGAATAACATCATAACATAGCCGCAAACATTTCGTTTGCGGTCATAGCGTGGGTGCCGCCTGGCAAGTGCTTATTTTCA                         >  SRR4302535.2201562/1‑94 (MQ=60)
                                                                       AACACATACGCATCCGAATAACATCATAACATAGCCGCAAACATTTCGTTTGCGGTCATAGCGTGGGTGCCGCCTGGCAAGTGCTTATTTTCAG                        <  SRR4302535.349553/94‑1 (MQ=60)
                                                                       AACACATACGCATCCGAATAACATCATAACATAGCCGCAAACATTTCGTTTGCGGTCATAGCGTGGGTGCCGCCTGGCAAGTGCTTATTTTCAG                        <  SRR4302535.2071891/94‑1 (MQ=60)
                                                                         CACATACGCATCCGAATAACATCATAACATAGCCGCAAACATTTCGTTTGCGGTCATAGCGTGGGTGCCGCCTGGCAAGTGCTTATTTTCAGGG                      >  SRR4302535.1428190/1‑94 (MQ=60)
                                                                         CACATACGCATCCGAATAACATCATAACATAGCCGCAAACATTTCGTTTGCGGTCATAGCGTGGGTGCCGCCTGGCAAGTGCTTATTTTCAGGG                      >  SRR4302535.299867/1‑94 (MQ=60)
                                                                         CACATACGCATCCGAATAACATCATAACATAGCCGCAAACATTTCGTTTGCGGTCATAGCGTGGGTGCCGCCTGGCAAGTGCTTATTTTCAGGG                      >  SRR4302535.3340506/1‑94 (MQ=60)
                                                                          ACATACGCATCCGAATAACATCATAACATAGCCGCAAACATTTCGTTTGCGGTCATAGCGTGGGTGCCGCCTGGCAAGTGCTTATTTTCAGGGG                     >  SRR4302535.1966306/1‑94 (MQ=60)
                                                                          ACATACGCATCCGAATAACATCATAACATAGCCGCAAACATTTCGTTTGCGGTCATAGCGTGGGTGCCGCCTGGCAAGTGCTTATTTTCAGGGG                     <  SRR4302535.4093928/94‑1 (MQ=60)
                                                                           CATACGCATCCGAATAACATCATAACATAGCCGCAAACATTTCGTTTGCGGTCATAGCGTGGGTGCCGCCTGGCAAGTGCTTATTTTCAGGGGT                    >  SRR4302535.21339/1‑94 (MQ=60)
                                                                            ATACGCATCCGAATAACATCATAACATAGCCGCAAACATTTCGTTTGCGGTCATAGCGTGGGTGCCGCCTGGCAAGTGCTTATTTTCAGGGGTA                   <  SRR4302535.71551/94‑1 (MQ=60)
                                                                              ACGCATCCGAATAACGTCATAACATAGCCGCAAACATTTCGTTTGCGGTCATAGCGTGGGTGCCGCCTGGCAAGTGCTTATTTTCAGGGGTATT                 <  SRR4302535.232491/94‑1 (MQ=60)
                                                                              ACGCATCCGAATAACATCATAACATAGCCGCAAACATTTCGTTTGCGGTCATAGCGTGGGTGCCGCCTGGCAAGTGCTTATTTTCAGGGGTATT                 <  SRR4302535.837148/94‑1 (MQ=60)
                                                                              ACGCATCCGAATAACATCATAACATAGCCGCAAACATTTCGTTTGCGGTCATAGCGTGGGTGCCGCCTGGCAAGTGCTTATTTTCAGGGGTATT                 <  SRR4302535.409309/94‑1 (MQ=60)
                                                                               CGCATCCGAATAACATCATAACATAGCCGCAAACATTTCGTTTGCGGTCATAGCGTGGGTGCCGCCTGGCAAGTGCTTATTTTCAGGGGTATTT                <  SRR4302535.1715171/94‑1 (MQ=60)
                                                                                GCATCCGAATAACATCATAACATAGCCGCAAACATTTCGTTTGCGGTCATAGCGTGGGTGCCGCCTGGCAAGTGCTTATTTTCAGGGGTATTTT               >  SRR4302535.2333579/1‑94 (MQ=60)
                                                                                 CATCCGAATAACATCATAACATAGCCGCAAACATTTCGTTTGCGGTCATAGCGTGGGTGCCGCCTGGCAAGTGCTTATTTTCAGGGGTATTTTG              >  SRR4302535.3163383/1‑94 (MQ=60)
                                                                                 CATCCGAATAACATCATAACATAGCCGCAAACATTTCGTTTGCGGTCATAGCGTGGGTGCCGCCTGGCAAGTGCTTATTTTCAGGGGTATTTTG              >  SRR4302535.319025/1‑94 (MQ=60)
                                                                                 CATCCGAATAACATCATAACATAGCCGCAAACATTTCGTTTGCGGTCATAGCGTGGGTGCCGCCTGGCAAGTGCTTATTTTCAGGGGTATTTTG              >  SRR4302535.1638220/1‑94 (MQ=60)
                                                                                  ATCCGAATAACATCATAACATAGCCGCAAACATTTCGTTTGCGGTCATAGCGTGGGTGCCGCCTGGCAAGTGCTTATTTTCAGGGGTATTTTGT             >  SRR4302535.1355873/1‑94 (MQ=60)
                                                                                   TCCGAATAACATCATAACATAGCCGCAAACATTTCGTTTGCGGTCATAGCGTGGGTGCCGCCTGGCAAGTGCTTATTTTCAGGGGTATTTTGTA            >  SRR4302535.4702863/1‑94 (MQ=60)
                                                                                   TCCGAATAACATCATAACATAGCCGCAAACATTTCGTTTGCGGTCATAGCGTGGGTGCCGCCTGGCAAGTGCTTATTTTCAGGGGTATTTTGTA            <  SRR4302535.3188917/94‑1 (MQ=60)
                                                                                   TCCGAATAACATCATAACATAGCCGCAAACATTTCGTTTGCGGTCATAGCGTGGGGGCCGCCTGGCAAGTGCTTATTTTCAGGGGTATTTTGTA            >  SRR4302535.3102901/1‑94 (MQ=60)
                                                                                    CCGAATAACATCATAACATAGCCGCAAACATTTCGTTTGCGGTCATAGCGTGGGTGCCGCCTGGCAAGTGCTTATTTTCAGGGGTATTTTGTAA           >  SRR4302535.3511325/1‑94 (MQ=60)
                                                                                      GAATAACATCATAACATAGCCGCAAACATTTCGTTTGCGGTCATAGCGTGGGTGCCGCCTGGCAAGTGCTTATTTTCAGGGGTATTTTGTAACA         <  SRR4302535.3994899/94‑1 (MQ=60)
                                                                                      GAATAACATCATAACATAGCCGCAAACATTTCGTTTGCGGTCATAGCGTGGGTGCCGCCTGGCAAGTGCTTATTTTCAGGGGTATTTTGTAACA         >  SRR4302535.266592/1‑94 (MQ=60)
                                                                                       AATAACATCATAACATAGCCGCAAACATTTCGTTTGCGGTCATAGCGTGGGTGCCGCCTGGCAAGTGCTTATTTTCAGGGGTATTTTGTAACAT        >  SRR4302535.2423169/1‑94 (MQ=60)
                                                                                        ATAACATCATAACATAGCCGCAAACATTTCGTTTGCGGTCATAGCGTGGGTGCCGCCTGGCAAGTGCTTATTTTCAGGGGTATTTTGTAACATG       <  SRR4302535.4537026/94‑1 (MQ=60)
                                                                                        ATAACATCATAACATAGCCGCAAACATTTCGTTTGCGGTCATAGCGTGGGTGCCGCCTGGCAAGTGCTTATTTTCAGGGGTATTTTGTAACATG       <  SRR4302535.389099/94‑1 (MQ=60)
                                                                                        ATAACATCATAACATAGCCGCAAACATTTCGTTTGCGGTCATAGCGTGGGTGCCGCCTGGCAAGTGCTTATTTTCAGGGGTATTTTGTAACATG       <  SRR4302535.4372846/94‑1 (MQ=60)
                                                                                         TAACATCATAACATAGCCGCAAACATTTCGTTTGCGGTCATAGCGTGGGTGCCGCCTGGCAAGTGCTTATTTTCAGGGGTATTTTGTAACATGG      <  SRR4302535.3100729/94‑1 (MQ=60)
                                                                                         TAACATCATAACATAGCCGCAAACATTTCGTTTGCGGTCATAGCGTGGGTGCCGCCTGGCAAGTGCTTATTTTCAGGGGTATTTTGTAACATGG      <  SRR4302535.1406067/94‑1 (MQ=60)
                                                                                         TAACATCATAACATAGCCGCAAACATTTCGTTTGCGGTCATAGCGTGGGTGCCGCCTGGCAAGTGCTTATTTTCAGGGGTATTTTGTAACATGG      <  SRR4302535.4573090/94‑1 (MQ=60)
                                                                                          AACATCATAACATAGCCGCAAACATTTCGTTTGCGGTCATAGCGTGGGTGCCGCCTGGCAAGTGCTTATTTTCAGGGGTATTTTGTAACATGGC     >  SRR4302535.43758/1‑94 (MQ=60)
                                                                                          AACATCATAACATAGCCGCAAACATTTCGTTTGCGGTCATAGCGTGGGTGCCGCCTGGCAAGTGCTTATTTTCAGGGGTATTTTGTAACATGGC     >  SRR4302535.886102/1‑94 (MQ=60)
                                                                                           ACATCATAACATAGCCGCAAACATTTCGTTTGCGGTCATAGCGTGGGTGCCGCCTGGCAAGTGCTTATTTTCAGGGGTATTTTGTAACATGGCA    >  SRR4302535.846622/1‑94 (MQ=60)
                                                                                            CATCATAACATAGCCGCAAACATTTCGTTTGCGGTCATAGCGTGGGTGCCGCCTGGCAAGTGCTTATTTTCAGGGGTATTTTGTAACATGGCAG   >  SRR4302535.1028065/1‑94 (MQ=60)
                                                                                            CATCATAACATAGCCGCAAACATTTCGTTTGCGGTCATAGCGTGGGTGCCGCCTGGCAAGTGCTTATTTTCAGGGGTATTTTGTAACATGGCAG   >  SRR4302535.3941078/1‑94 (MQ=60)
                                                                                            CATCATAACATAGCCGCAAACATTTCGTTTGCGGTCATAGCGTGGGTGCCGCCTGGCAAGTGCTTATTTTCAGGGGTATTTTGTAACATGGCAG   >  SRR4302535.2041121/1‑94 (MQ=60)
                                                                                             ATCATAACATAGCCGCAAACATTTCGTTTGCGGTCATAGCGTGGGTGCCGCCTGGCAAGTGCTTATTTTCAGGGGTATTTTGTAACATGGCAGA  >  SRR4302535.4499846/1‑94 (MQ=60)
                                                                                             ATCATAACATAGCCGCAAACATTTCGTTTGCGGTCATAGCGTGGGTGCCGCCTGGCAAGTGCTTATTTTCAGGGGTATTTTGTAACATGGCAGA  <  SRR4302535.1737992/94‑1 (MQ=60)
                                                                                             ATCATAACATAGCCGCAAACATTTCGTTTGCGGTCATAGCGTGGGTGCCGCCTGGCAAGTGCTTATTTTCAGGGGTATTTTGTAACATGGCAGA  >  SRR4302535.566604/1‑94 (MQ=60)
                                                                                             ATCATAACATAGCCGCAAACATTTCGTTTGCGGTCATAGCGTGGGTGCCGCCTGGCAAGTGCTTATTTTCAGGGGTATTTTGTAACATGGCAGA  <  SRR4302535.4175272/94‑1 (MQ=60)
                                                                                             ATCATAACATAGCCGCAAACATTTCGTTTGCGGTCATAGCGTGGGTGCCGCCTGGCAAGTGCTTATTTTCAGGGGTATTTTGTAACATGGCAGA  >  SRR4302535.1122174/1‑94 (MQ=60)
                                                                                             ATCATAACATAGCCGCAAACATTTCGTTTGCGGTCATAGCGTGGGTGCCGCCTGGCAAGTGCTTATTTTCAGGGGTATTTTGTAACATGGCAGA  >  SRR4302535.1217102/1‑94 (MQ=60)
                                                                                             ATCATAACATAGCCGCAAACATTTCGTTTGCGGTCATAGCGTGGGTGCCGCCTGGCAAGGGCTTATTTTCAGGGGTATTTTGTAACATGGCAGA  >  SRR4302535.2797832/1‑94 (MQ=60)
                                                                                             |                                                                                             
ATTTAATCTGGTGGAAGATTTCGACGCCACCGACCGCGGTGAAGGCGGCTTTGGTCACTCTGGTCGTCAGTAACACATACGCATCCGAATAACGTCATAACATAGCCGCAAACATTTCGTTTGCGGTCATAGCGTGGGTGCCGCCTGGCAAGTGCTTATTTTCAGGGGTATTTTGTAACATGGCAGA  >  NC_000913/3811942‑3812128

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑