Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 1873 183.1 9376148 98.2% 9207377 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,838,693 A→G I280T (ATC→ACC)  yicM ← putative transport protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,838,6930AG99.6% 744.3 / ‑5.2 236I280T (ATC→ACC) yicMputative transport protein
Reads supporting (aligned to +/- strand):  ref base A (1/0);  new base G (108/127);  total (109/127)
Fisher's exact test for biased strand distribution p-value = 4.62e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.67e-01

Only 100 of 240 total aligned reads displayed.

GTTAAGCCATCCACGCCGAATCCCGCCAGGTTCATATACACCGGGCGAATATACGTGAAGAAAGCAAACTGCCCGGCGAAAGACATGAAGATGGCGATCATCCCTGCCATCACACCCGGACGTTGTAATAAGCGGAAAGTATTTTGTTTCTGATGCGAGGGTTCGCCTGGCAGTGAAGGC  >  NC_000913/3838603‑3838782
                                                                                          |                                                                                         
gTTAAGCCATCCACGCCGAATCCCGCCAGGTTCATATACACCGGGCGAATATACGTGAAGAAAGCAAACTGCCCGGCGAAAGACATGAAGGTg                                                                                         <  1:4284189/93‑1 (MQ=255)
  tAAGCCATCCACGCCGAATCCCGCCAGGTTCATATACACCGGGCGAATATACGTGAAGAAAGCAAACTGCCCGGCGAAAGACATGAAGGTGGc                                                                                       <  2:3448023/93‑1 (MQ=255)
     gCCATCCACGCCGAATCCCGCCAGGTTCATATACACCGGGCGAATATACGTGAAGAAAGCAAACTGCCCGGCGAAAGACATGAAGGTGGCGat                                                                                    <  2:4339608/93‑1 (MQ=255)
      ccATCCACGCCGAATCCCGCCAGGTTCATATACACCGGGCGAATATACGTGAAGAAAGCAAACTGCCCGGCGAAAGACATGAAGGTGGCGatc                                                                                   >  1:766710/1‑93 (MQ=255)
      ccATCCACGCCGAATCCCGCCAGGTTCATATACACCGGGCGAATATACGTGAAGAAAGCAAACTGCCCGGCGAAAGACATGAAGGTGGCGatc                                                                                   <  1:1669204/93‑1 (MQ=255)
       cATCCACGCCGAATCCCGCCAGGTTCATATACACCGGGCGAATATACGTGAAGAAAGCAAACTGCCCGGCGAAAGACATGAAGGTGGCGatca                                                                                  <  1:3529116/93‑1 (MQ=255)
       cATCCACGCCGAATCCCGCCAGGTTCATATACACCCGGCGAATATACGTGAAGAAAGCAAACTGCCCGGCGAAAGACATGAAGGTGGCGatca                                                                                  >  2:860656/1‑93 (MQ=255)
        aTCCACGCCGAATCCCGCCAGGTTCATATACACCGGGCGAATATACGTGAAGAAAGCAAACTGCCCGGCGAAAGACATGAAGGTGGCGatcat                                                                                 >  1:584904/1‑93 (MQ=255)
        aTCCACGCCGAATCCCGCCAGGTTCATATACACCGGGCGAATATACGTGAAGAAAGCAAACTGCCCGGCGAAAGACATGAAGGTGGCGatcat                                                                                 >  2:214708/1‑93 (MQ=255)
        aTCCACGCCGAATCCCGCCAGGTTCATATACACCGGGCGAATATACGTGAAGAAAGCAAACTGCCCGGCGAAAGACATGAAGGTGGCGatcat                                                                                 >  2:3874503/1‑93 (MQ=255)
         tCCACGCCGAATCCCGCCAGGTTCATATACACCGGGCGAATATACGTGAAGAAAGCAAACTGCCCGGCGAAAGACATGAAGGTGGCGatcatc                                                                                <  2:1976062/93‑1 (MQ=255)
          ccACGCCGAATCCCGCCAGGTTCATATACACCGGGCGAATATACGTGAAGAAAGCAAACTGCCCGGCGAAAGACATGAAGGTGGCGATCATcc                                                                               <  1:4018647/93‑1 (MQ=255)
           cACGCCGAATCCCGCCAGGTTCATATACACCGGGCGAATATACGTGAAGAAAGCAAACTGCCCGGCGAAAGACATGAAGGTGGCGATCATccc                                                                              >  2:916965/1‑93 (MQ=255)
           cACGCCGAATCCCGCCAGGTTCATATACACCGGGCGAATATACGTGAAGAAAGCAAACTGCCCGGCGAAAGACATGAAGGTGGCGATCATccc                                                                              <  1:3237559/93‑1 (MQ=255)
              gCCGAATCCCGCCAGGTTCATATACACCGGGCGAATATACGTGAAGAAAGCAAACTGCCCGGCGAAAGACATGAAGGTGGCGATCATCCCTGc                                                                           <  1:4658959/93‑1 (MQ=255)
                cGAATCCCGCCAGGTTCATATACACCGGGCGAATATACGTGAAGAAAGCAAACTGCCCGGCGAAAGACATGAAGGTGGCGATCATCCCTGCCa                                                                         <  1:2097353/93‑1 (MQ=255)
                 gAATCCCGCCAGGTTCATATACACCGGGCGAATATACGTGAAGAAAGCAAACTGCCCGGCGAAAGACATGAAGGTGGCGATCATCCCTGCCAt                                                                        >  2:770386/1‑93 (MQ=255)
                    acccGCCAGGTTCATATACACCGGGCGAATATACGTGAAGAAAGCAAACTGCCCGGCGAAAGACATGAAGGTGGCGATCATCCCTGCCATcac                                                                     <  1:315269/92‑1 (MQ=255)
                      ccGCCAGGTTCATATACACCGGGCGAATATACGTGAAGAAAGCAAACTGCCCGGCGAAAGACATGAAGGTGGCGATCATCCCTGCCATCacac                                                                   >  2:1020753/1‑93 (MQ=255)
                       cGCCAGGTTCATATACACCGGGCGAATATACGTGAAGAAAGCAAACTGCCCGGCGAAAGACATGAAGGTGGCGATCATCCCTGCCATCACAcc                                                                  <  2:2281770/93‑1 (MQ=255)
                       cGCCAGGTTCATATACACCGGGCGAATATACGTGAAGAAAGCAAACTGCCCGGCGAAAGACATGAAGGTGGCGATCATCCCTGCCATCACAcc                                                                  <  1:3400618/93‑1 (MQ=255)
                         ccAGGTTCATATACACCGGGCGAATATACGTGAAGAAAGCAAACTGCCCGGCGAAAGACATGAAGGTGGCGATCATCCCTGCCATCACACCCg                                                                <  2:3209661/93‑1 (MQ=255)
                         ccAGGTTCATATACACCGGGCGAATATACGTGAAGAAAGCAAACTGCCCGGCGAAAGACATGAAGATGGCGATCATCCCTGCCATCACACCCg                                                                >  1:1951330/1‑93 (MQ=255)
                          cAGGTTCATATACACCGGGCGAATATACGTGAAGAAAGCAAACTGCCCGGCGAAAGACATGAAGGTGGCGATCATCCCTGCCATCACACCCgg                                                               >  1:4522407/1‑93 (MQ=255)
                          cAGGTTCATATACACCGGGCGAATATACGTGAAGAAAGCAAACTGCCCGGCGAAAGACATGAAGGTGGCGATCATCCCTGCCATCACACCCgg                                                               >  2:681676/1‑93 (MQ=255)
                           aGGTTCATATACACCGGGCGAATATACGTGAAGAAAGCAAACTGCCCGGCGAAAGACATGAAGGTGGCGATCATCCCTGCCATCACACCCGGa                                                              >  1:1702270/1‑93 (MQ=255)
                           aGGTTCATATACACCGGGCGAATATACGTGAAGAAAGCAAACTGCCCGGCGAAAGACATGAAGGTGGCGATCATCCCTGCCATCACACCCGGa                                                              >  2:2240632/1‑93 (MQ=255)
                           aGGTTCATATACACCGGGCGAATATACGTGAAGAAAGCAAACTGCCCGGCGAAAGACATGAAGGTGGCGATCATCCCTGCCATCACACCCGGa                                                              <  1:2974833/93‑1 (MQ=255)
                           aGGTTCATATACACCGGGCGAATATACGTGAAGAAAGCAAACTGCCAGGCGAAAGACATGAAGGTGGCGATCATCCCTGCCATCACACCCGGa                                                              <  1:3122585/93‑1 (MQ=255)
                             gTTCATATACACCGGGCGAATATACGTGAAGAAAGCAAACTGCCCGGCGAAAGACATGAAGGTGGCGATCATCCCTGCCATCACACCCGGACg                                                            <  1:900798/93‑1 (MQ=255)
                             gTTCATATACACCGGGCGAATATACGTGAAGAAAGCAAACTGCCCGGCGAAAGACATGAAGGTGGCGATCATCCCTGCCATCACACCCGGACg                                                            >  2:4218848/1‑93 (MQ=255)
                              ttCATATACACCGGGCGAATATACGTGAAGAAAGCAAACTGCCCGGCGAAAGACATGAAGGTGGCGATCATCCCTGCCATCACACCCGGACGt                                                           <  1:884063/93‑1 (MQ=255)
                              ttCATATACACCGGGCGAATATACGTGAAGAAAGCAAACTGCCCGGCGAAAGACATGAAGGTGGCGATCATCCCTGCCATCACACCCGGACGt                                                           <  1:684138/93‑1 (MQ=255)
                               tCATATACACCGGGCGAATATACGTGAAGAAAGCAAACTGCCCGGCGAAAGACATGAAGGTGGCGATCATCCCTGCCATCACACCCGGACGtt                                                          <  1:538252/93‑1 (MQ=255)
                               tCATATACACCGGGCGAATATACGTGAAGAAAGCAAACTGCCCGGCGAAAGACATGAAGGTGGCGATCATCCCTGCCATCACACCCGGACGtt                                                          <  2:1474021/93‑1 (MQ=255)
                                 atatACACCGGGCGAATATACGTGAAGAAAGCAAACTGCCCGGCGAAAGACATGAAGGTGGCGATCATCCCTGCCATCACACCCGGACGTTGt                                                        >  2:1808592/1‑93 (MQ=255)
                                  tataCACCGGGCGAATATACGTGAAGAAAGCAAACTGCCCGGCGAAAGACATGAAGGTGGCGATCATCCCTGCCATCACACCCGGACGTTGta                                                       >  2:142167/1‑93 (MQ=255)
                                   ataCACCGGGCGAATATACGTGAAGAAAGCAAACTGCCCGGCGAAAGACATGAAGGTGGCGATCATCCCTGCCATCACACCCGGACGTTGtaa                                                      <  2:1275664/93‑1 (MQ=255)
                                    tccacCGGGCGAAAAAACGTGAAGAAAGCAAACTGCCCGGCGAAAGACATGAAGGTGGCGATCATCCCTGCCATCACACCCGGACGTTGtagt                                                     <  2:3735865/91‑3 (MQ=255)
                                    taCACCGGGCGAATATACGTGAAGAAAGCAAACTGCCCGGCGAAAGACATGAAGGTGGCGATCATCCCTGCCATCACACCCGGACGTTGtaat                                                     >  1:1598192/1‑93 (MQ=255)
                                      cacCGGGCGAATATACGTGAAGAAAGCAAACTGCCCGGCGAAAGACATGAAGGTGGCGATCATCCCTGCCATCACACCCGGACGTTGtaataa                                                   <  2:453832/93‑1 (MQ=255)
                                        ccGGGCGAATATACGTGAAGAAAGCAAACTGCCCGGCGAAAGACATGAAGGTGGCGATCATCCCTGCCATCACACCCGGACGTTGTAATAAGc                                                 <  1:2564572/93‑1 (MQ=255)
                                         cGGGCGAATATACGTGAAGAAAGCAAACTGCCCGGCGAAAGACATGAAGGTGGCGATCATCCCTGCCATCACACCCGGACGTTGTAATAAGCg                                                >  2:2082966/1‑93 (MQ=255)
                                             cGAATATACGTGAAGAAAGCAAACTGCCCGGCGAAAGACATGAAGGTGGCGATCATCCCTGCCATCACACCCGGACGTTGTAATAAGCGGaaa                                            <  1:1293844/93‑1 (MQ=255)
                                              gAATATACGTGAAGAAAGCAAACTGCCCGGCGAAAGACATGAAGGTGGCGATCATCCCTGCCATCACACCCGGACGTTGTAATAAGCGGAAAg                                           <  2:2928177/93‑1 (MQ=255)
                                                atatACGTGAAGAAAGCAAACTGCCCGGCGAAAGACATGAAGGTGGCGATCATCCCTGCCATCACACCCGGACGTTGTAATAAGCGGAAAGTa                                         <  2:3919437/93‑1 (MQ=255)
                                                atatACGTGAAGAAAGCAAACTGCCCGGCGAAAGACATGAAGGTGGCGATCATCCCTGCCATCACACCCGGACGTTGTAATAAGCGGAAAGTa                                         >  1:2218194/1‑93 (MQ=255)
                                                atatACGTGAAGAAAGCAAACTGCCCGGCGAAAGACATGAAGGTGGCGATCATCCCTGCCATCACACCCGGACGTTGTAATAAGCGGAAAGTa                                         >  1:1249347/1‑93 (MQ=255)
                                                atatACGTGAAGAAAGCAAACTGCCCGGCGAAAGACATGAAGGTGGCGATCATCCCTGCCATCACACCCGGACGTTGTAATAAGCGGAAAGTa                                         >  2:614689/1‑93 (MQ=255)
                                                atatACGTGAAGAAAGCAAACTGCCCGGCGAAAGACATGAAGGTGGCGATCATCCCTGCCATCACACCCGGACGTTGTAATAAGCGGAAAGTa                                         >  1:2721594/1‑93 (MQ=255)
                                                atatACGTGAAGAAAGCAAACTGCCCGGCGAAAGACATGAAGGTGGCGATCATCCCTGCCATCACACCCGGACGTTGTAATAAGCGGAAAGTa                                         <  1:2761380/93‑1 (MQ=255)
                                                 tataCGTGAAGAAAGCAACCTGCCCGGCGAAAGACATGAAGGTGGCGATCATCCCTGCCATCACACCCGGACGTTGTAATAAGCGGAAAGTAt                                        <  2:477072/93‑1 (MQ=255)
                                                  ataCGTGAAGAAAGCAAACTGCCCGGCGAAAGACATGAAGGTGGCGATCATCCCTGCCATCACACCCGGACGTTGTAATAAGCGGAAAGTAtt                                       <  2:3088860/93‑1 (MQ=255)
                                                   taCGTGAAGAAAGCAAACTGCCCGGCGAAAGACATGAAGGTGGCGATCATCCCTGCCATCACACCCGGACGTTGTAATAAGCGGAAAGTAttt                                      >  2:57023/1‑93 (MQ=255)
                                                    aCGTGAAGAAAGCAAACTTCCCGGCGAAAGACATGAAGGTGGCGATCATCCCTGCCATCACACCCGGACGTTGTAATAAGCGGAAAGTAtttt                                     <  1:1146594/93‑1 (MQ=255)
                                                      gTGAAGAAAGCAAACTGCCCGGCGAAAGACATGAAGGTGGCGATCATCCCTGCCATCACACCCGGACGTTGTAATAAGCGGAAAGTATTTTGt                                   >  1:1898654/1‑93 (MQ=255)
                                                       tGAAGAAAGCAAACTGCCCGGCGAAAGACATGAAGGTGGCGATCATCCCTGCCATCACACCCGGACGTTGTAATAAGCGGAAAGTATTTTGtt                                  >  1:3601551/1‑93 (MQ=255)
                                                        gaagaaAGCAAACTGCCCGGCGAAAGACATGAAGGTGGCGATCATCCCTGCCATCACACCCGGACGTTGTAATAAGCGGAAAGTATTTTGttt                                 >  1:703817/1‑93 (MQ=255)
                                                        gaagaaAGCAAACTGCCCGGCGAAAGACATGAAGGTGGCGATCATCCCTGCCATCACACCCGGACGTTGTAATAAGCGGAAAGTATTTTGttt                                 <  1:3653469/93‑1 (MQ=255)
                                                        gaagaaAGCAAACTGCCCGGCGAAAGACATGAAGGTGGCGATCATCCCTGCCATCACACCCGGACGTTGTAATAAGCGGAAAGTATTTTGttt                                 >  1:4322796/1‑93 (MQ=255)
                                                         aagaaAGCAAACTGCCCGGCGAAAGACATGAAGGTGGCGATCATCCCTGCCATCACACCCGGACGTTGTAATAAGCGGAAAGTATTTTGTTTc                                <  1:4217672/93‑1 (MQ=255)
                                                          agaaAGCAAACTGCCCGGCGAAAGACATGAAGGTGGCGATCATCCCTGCCATCACACCCGGACGTTGTAATAAGCGGAAAGTATTTTGTTTCt                               <  2:3312395/93‑1 (MQ=255)
                                                           gaaAGCAAACTGCCCGGCGAAAGACATGAAGGTGGCGATCATCCCTGCCATCACACCCGGACGTTGTAATAAGCGGAAAGTATTTTGTTTCTg                              >  1:2008700/1‑93 (MQ=255)
                                                            aaaGCAAACTGCCCGGCGAAAGACATGAAGGTGGCGATCATCCCTGCCATCACACCCGGACGTTGTAATAAGCGGAAAGTATTTTGTTTCTGa                             <  1:2890468/93‑1 (MQ=255)
                                                             aaGCAAACTGCCCGGCGAAAGACATGAAGGTGGCGATCATCCCTGCCATCACACCCGGACGTTGTAATAAGCGGAAAGTATTTTGTTTCTGAt                            <  2:3411303/93‑1 (MQ=255)
                                                             aaGCAAACTGCCCGGCGAAAGACATGAAGGTGGCGATCATCCCTGCCATCACACCCGGACGTTGTAATAAGCGGAAAGTATTTTGTTTCTGAt                            <  2:4141127/93‑1 (MQ=255)
                                                             aaGCAAACTGCCCGGCGAAAGACATGAAGGTGGCGATCATCCCTGCCATCACACCCGGACGTTGTAATAAGCGGAAAGTATTTTGTTTCTGAt                            >  1:659798/1‑93 (MQ=255)
                                                             aaGCAAACTGCCCGGCGAAAGACATGAAGGTGGCGATCATCCCTGCCATCACACCCGGACGTTGTAATAAGCGGAAAGTATTTTGTTTCTGAt                            <  2:2466194/93‑1 (MQ=255)
                                                              aGCAAACTGCCCGGCGAAAGACATGAAGGTGGCGATCATCCCTGCCATCACACCCGGACGTTGTAATAAGCGGAAAGTATTTTGTTTCTGATg                           >  2:2865764/1‑93 (MQ=255)
                                                               gCAAACTGCCCGGCGAAAGACATGAAGGTGGCGATCATCCCTGCCATCACACCCGGACGTTGTAATAAGCGGAAAGTATTTTGTTTCTGATGc                          >  1:1620540/1‑93 (MQ=255)
                                                                cAAACTGCCCGGCGAAAGACATGAAGGTGGCGATCATCCCTGCCATCACACCCGGACGTTGTAATAAGCGGAAAGTATTTTGTTTCTGATGCg                         <  1:4047923/93‑1 (MQ=255)
                                                                cAAACTGCCCGGCGAAAGACATGAAGGTGGCGATCATCCCTGCCATCACACCCGGACGTTGTAATAAGCGGAAAGTATTTTGTTTCTGATGCg                         >  2:3156222/1‑93 (MQ=255)
                                                                 aaaCTGCCCGGCGAAAGACATGAAGGTGGCGATCATCCCTGCCATCACACCCGGACGTTGTAATAAGCGGAAAGTATTTTGTTTCTGATGCGa                        >  2:444769/1‑93 (MQ=255)
                                                                    cTGCCCGGCGAAAGACATGAAGGTGGCGATCATCCCTGCCATCACACCCGGACGTTGTAATAAGCGGAAAGTATTTTGTTTCTGATGCGAggg                     <  2:4531890/93‑1 (MQ=255)
                                                                     tGCCCGGCGAAAGACATGAAGGTGGCGATCATCCCTGCCATCACACCCGGACGTTGTAATAAGCGGAAAGTATTTTGTTTCTGATGCGAGGGt                    <  1:4634539/93‑1 (MQ=255)
                                                                      gCCCGGCGAAAGACATGAAGGTGGCGATCATCCCTGCCATCACACCCGGACGTTGTAATAAGCGGAAAGTATTTTGTTTCTGATGCGAGGGtt                   <  1:2112050/93‑1 (MQ=255)
                                                                      gCCCGGCGAAAGACATGAAGGTGGCGATCATCCCTGCCATCACACCCGGACGTTGTAATAAGCGGAAAGTATTTTGTTTCTGATGCGAGGGtt                   >  1:1017590/1‑93 (MQ=255)
                                                                       cccGGCGAAAGACATGAAGGTGGCGATCATCCCTGCCATCACACCCGGACGTTGTAATAAGCGGAAAGTATTTTGTTTCTGATGCGAGGGTTc                  <  1:1824559/93‑1 (MQ=255)
                                                                           gCGAAAGACATGAAGGTGGCGATCATCCCTGCCATCACGCCCGGACGTTGTAATAAGCGGAAAGTATTTTGTTTCTGATGCGAGGGTTCGCCt              <  2:3644703/93‑1 (MQ=255)
                                                                             gAAAGACATGAAGGTGGCGATCATCCCTGCCATCACACCCGGACGTTGTAATAAGCGGAAAGTATTTTGTTTCTGATGCGAGGGTTCGCCTgg            <  2:2713122/93‑1 (MQ=255)
                                                                              aaaGACATGAAGGTGGCGATCATCCCTGCCATCACACCCGGACGTTGTAATAAGCGGAAAGTATTTTGTTTCTGATGCGAGGGTTCGCCTGGc           >  1:1631661/1‑93 (MQ=255)
                                                                              aaaGACATGAAGGTGGCGATCATCCCTGCCATCACACCCGGACGTTGTAATAAGCGGAAAGTATTTTGTTTCTGATGCGAGGGTTCGCCTGGc           >  1:4186168/1‑93 (MQ=255)
                                                                               aaGACATGAAGGTGGCGATCATCCCTGCCATCACACCCGGACGTTGTAATAAGCGGAAAGTATTTTGTTTCTGATGCGAGGGTTCGCCTGGCa          <  2:4270259/93‑1 (MQ=255)
                                                                               aaGAAATGAGGGTGGCGTCCATCCCTGCCATAACACCCGGACGTTGTAATAAGCGGAAAGTATTTTGTTTCTGATGCGAGGGTTCGCCTGGCa          <  1:4429536/93‑1 (MQ=255)
                                                                                aGACATGAAGGTGGCGATCATCCCTGCCATCACACCCGGACGTTGTAATAAGCGGAAAGTATTTTGTTTCTGATGCGAGGGTTCGCCTGGCAg         <  1:2071764/93‑1 (MQ=255)
                                                                                aGACATGAAGGTGGCGATCATCCCTGCCATCACACCCGGACGTTGTAATAAGCGGAAAGTATTTTGTTTCTGATGCGAGGGTTCGCCTGGCAg         >  1:4494898/1‑93 (MQ=255)
                                                                                 gACATGAAGGTGGCGATCATCCCTGCCATCACACCCGGGCGTTGTAATAAGCGGAAAGGATTTTGTTTCTGATGCGAGGGGTCCGCTGGGAGt        >  1:1799590/1‑93 (MQ=255)
                                                                                 gACATGAAGGTGGCGATCATCCCTGCCATCACACCCGGACGTTGTAATAAGCGGAAAGTATTTTGTTTCTGATGCGAGGGTTCGCCTGGCAGt        >  1:1453167/1‑93 (MQ=255)
                                                                                   cATGAAGGTGGCGATCATCCCTGCCATCACACCCGGACGTTGTAATAAGCGGAAAGTATTTTGTTTCTGATGCGAGGGTTCGCCTGGCAGTGa      >  2:2025231/1‑93 (MQ=255)
                                                                                   cATGAAGGTGGCGATCATCCCTGCCATCACACCCGGACGTTGTAATAAGCGGAAAGCATTTTGATTCTGATGCGAGGGTTCGCCTGGCAGTGa      >  2:3022589/1‑93 (MQ=255)
                                                                                    aTGAAGGTGGCGATCATCCCTGCCATCACACCCGGACGTTGTAATAAGCGGAAAGTATTTTGTTTCTGATGCGAGGGTTCGCCTGGCAGTGaa     >  2:1186655/1‑93 (MQ=255)
                                                                                    aTGAAGGTGGCGATCATCCCTGCCATCACACCCGGACGTTGTAATAAGCGGAAAGTATTTTGTTTCTGATGCGAGGGTTCGCCTGGCAGTGaa     >  2:2615312/1‑93 (MQ=255)
                                                                                     tGAAGGTGGCGATCATCCCTGCCATCACACCCGGACGTTGTAATAAGCGGCAAGTATTTTGTTTCTGATGCGAGGGCTCGCCTGGCAGTGAAg    >  2:4465491/1‑93 (MQ=255)
                                                                                     tGAAGGTGGCGATCATCCCTGCCATCACACCCGGACGTTGTAATAAGCGGAAAGTATTTTGTTTCTGATGCGAGGGTTCGCCTGGCAGTGAAg    <  1:4155144/93‑1 (MQ=255)
                                                                                     tGAAGGTGGCGATCATCCCTGCCATCACACCCGGACGTTGTAATAAGCGGAAAGTATTTTGTTTCTGATGCGAGGGTTCGCCTGGCAGTGAAg    >  2:2156934/1‑93 (MQ=255)
                                                                                     tGAAGGTGGCGATCATCCCTGCCATCACACCCGGACGTTGTAATAAGCGGAAAGTATTTTGTTTCTGATGCGAGGGTTCGCCTGGCAGTGAAg    >  1:510280/1‑93 (MQ=255)
                                                                                      gAAGGTGGCGATCATCCCTGCCATCACACCCGGACGTTGTAATAAGCGGAAAGTATTTTGTTTCTGATGCGAGGGTTCGCCTGGCAGTGAAgg   <  2:3051625/93‑1 (MQ=255)
                                                                                      gAAGGTGGCGATCATCCCTGCCATCACACCCGGACGTTGTAATAAGCGGAAAGTATTTTGTTTCTGATGCGAGGGTTCGCCTGGCAGTGAAgg   <  1:2196084/93‑1 (MQ=255)
                                                                                      gAAGGTGGCGATCATCCCTGCCATCACACCCGGACGTTGTAATAAGCGGAAAGTATTTTGTTTCTGATGCGAGGGTTCGCCTGGCAGTGAAgg   >  2:3594971/1‑93 (MQ=255)
                                                                                       aaGGTGGCGATCATCCCTGCCATCACACCCGGACGTTGTAATAAGCGGAAAGTATTTTGTTTCTGATGCGAGGGTTCGCCTGGCAGTGAAGGc  <  1:3320790/93‑1 (MQ=255)
                                                                                          |                                                                                         
GTTAAGCCATCCACGCCGAATCCCGCCAGGTTCATATACACCGGGCGAATATACGTGAAGAAAGCAAACTGCCCGGCGAAAGACATGAAGATGGCGATCATCCCTGCCATCACACCCGGACGTTGTAATAAGCGGAAAGTATTTTGTTTCTGATGCGAGGGTTCGCCTGGCAGTGAAGGC  >  NC_000913/3838603‑3838782

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CGTTAAGCCATCCACGCCGAATCCCGCCAGGTTCATATACACCGGGCGAATATACGTGAAGAAAGCAAACTGCCCGGCGAAAGACATGAAGATGGCGATCATCCCTGCCAT‑CACACCCGGACGTTGTAATAAGCGGAAAGTATTTTGTTTCTGATGCGAGGGTTCGCCTGGCAGTGAAGGCAATG  >  NC_000913/3838602‑3838786
                                                                                           |                                                                                              
CGTTAAGCCATCCACGCCGAATCCCGCCAGGTTCATATACACCGGGCGAATATACGTGAAGAAAGCAAACTGCCCGGCGAAAGACATGAAGGTG                                                                                              <  SRR4302535.1152323/94‑1 (MQ=60)
CGTTAAGCCATCCACGCCGAATCCCGCCAGGTTCATATACACCGGGCGAATATACGTGAAGAAAGCAAACTGCCCGGCGAAAGACATGAAGGTG                                                                                              <  SRR4302535.2813798/94‑1 (MQ=60)
 GTTAAGCCATCCACGCCGAATCCCGCCAGGTTCATATACACCGGGCGAATATACGTGAAGAAAGCAAACTGCCCGGCGAAAGACATGAAGGTGG                                                                                             <  SRR4302535.4313498/94‑1 (MQ=60)
    AAGCCATCCACGCCGAATCCCGCCAGGTTCATATACACCGGGCGAATATACGTGAAGAAAGCAAACTGCCCGGCGAAAGACATGAAGGTGGCGA                                                                                          >  SRR4302535.22071/1‑94 (MQ=60)
     AGCCATCCACGCCGAATCCCGCCAGGTTCATATACACCGGGCGAATATACGTGAAGAAAGCAAACTGCCCGGCGAAAGACATGAAGGTGGCGAT                                                                                         >  SRR4302535.1076106/1‑94 (MQ=60)
      GCCATCCACGCCGAATCCCGCCAGGTTCATATACACCGGGCGAATATACGTGAAGAAAGCAAACTGCCCGGCGAAAGACATGAAGGTGGCGATC                                                                                        >  SRR4302535.772107/1‑94 (MQ=60)
      GCCATCCACGCCGAATCCCGCCAGGTTCATATACACCGGGCGAATATACGTGAAGAAAGCAAACTGCCCGGCGAAAGACATGAAGGTGGCGATC                                                                                        <  SRR4302535.1813151/94‑1 (MQ=60)
      GCCATCCACGCCGAATCCCGCCAGGTTCATATACACCGGGCGAATATACGTGAAGAAAGCAAACTGCCCGGCGAAAGACATGAAGGTGGCGATC                                                                                        <  SRR4302535.89501/94‑1 (MQ=60)
       CCATCCACGCCGAATCCCGCCAGGTTCATATACACCGGGCGAATATACGTGAAGAAAGCAAACTGCCCGGCGAAAGACATGAAGGTGGCGATCA                                                                                       <  SRR4302535.1681282/94‑1 (MQ=60)
        CATCCACGCCGAATCCCGCCAGGTTCATATACACCGGGCGAATATACGTGAAGAAAGCAAACTGCCCGGCGAAAGACATGAAGGTGGCGATCAT                                                                                      <  SRR4302535.3553622/94‑1 (MQ=60)
        CATCCACGCCGAATCCCGCCAGGTTCATATACACCGGGCGAATATACGTGAAGAAAGCAAACTGCCCGGCGAAAGACATGAAGGTGGCGATCAT                                                                                      >  SRR4302535.589004/1‑94 (MQ=60)
         ATCCACGCCGAATCCCGCCAGGTTCATATACACCGGGCGAATATACGTGAAGAAAGCAAACTGCCCGGCGAAAGACATGAAGGTGGCGATCATC                                                                                     >  SRR4302535.1305307/1‑94 (MQ=60)
          TCCACGCCGAATCCCGCCAGGTTCATATACACCGGGCGAATATACGTGAAGAAAGCAAACTGCCCGGCGAAAGACATGAAGGTGGCGATCATCC                                                                                    >  SRR4302535.780427/1‑94 (MQ=60)
          TCCACGCCGAATCCCGCCAGGTTCATATACACCGGGCGAATATACGTGAAGAAAGCAAACTGCCCGGCGAAAGACATGAAGGTGGCGATCATCC                                                                                    <  SRR4302535.3377806/94‑1 (MQ=60)
          TCCACGCCGAATCCCGCCAGGTTCATATACACCGGGCGAATATACGTGAAGAAAGCAAACTGCCCGGCGAAAGACATGAAGGTGGCGATCATCC                                                                                    <  SRR4302535.458629/94‑1 (MQ=60)
          TCCACGCCGAATCCCGCCAGGTTCATATACACCGGGCGAATATACGTGAAGAAAGCAAACTGACCGGCGCAAGACATGAAGGTGGCGATCATCC                                                                                    >  SRR4302535.1748674/1‑94 (MQ=60)
           CCACGCCGAATCCCGCCAGGTTCATATACACCGGGCGAATATACGTGAAGAAAGCAAACTGCCCGGCGAAAGACATGAAGGTGGCGATCATCCC                                                                                   <  SRR4302535.4046142/94‑1 (MQ=60)
            CACGCCGAATCCCGCCAGGTTCATATACACCGGGCGAATATACGTGAAGAAAGCAAACTGCCCGGCGAAAGACATGAAGGTGGCGATCATCCCT                                                                                  <  SRR4302535.739330/94‑1 (MQ=60)
            CACGCCGAATCCCGCCAGGTTCATATACACCGGGCGAATATACGTGAAGAAAGCAAACTGCCCGGCGAAAGACATGAAGGTGGCGATCATCCCT                                                                                  <  SRR4302535.3260034/94‑1 (MQ=60)
               GCCGAATCCCGCCAGGTTCATATACACCGGGCGAATATACGTGAAGAAAGCAAACTGCCCGGCGAAAGACATGAAGGTGGCGATCATCCCTGCC                                                                               <  SRR4302535.4690473/94‑1 (MQ=60)
                CCGAATCCCGCCAGGTTCATATACACCGGGCGAATATACGTGAAGAAAGCAAACTGCCCGGCGAAAGACATGAAGGTGGCGATCATCCCTGCCA                                                                              >  SRR4302535.1301605/1‑94 (MQ=60)
                 CGAATCCCGCCAGGTTCATATACACCGGGCGAATATACGTGAAGAAAGCAAACTGCCCGGCGAAAGACATGAAGGTGGCGATCATCCCTGCCAT                                                                             <  SRR4302535.2112479/94‑1 (MQ=60)
                 CGAATCCCGCCAGGTTCATATACACCGGGCGAATATACGTGAAGAAAGCAAACTGCCCGGCGAAAGACATGAAGGTGGCGATCATCCCTGCCAT                                                                             >  SRR4302535.4025483/1‑94 (MQ=60)
                     TCCCGCCAGGTTCATATACACCGGGCGAATATACGTGAAGAAAGCAAACTGCCCGGCGAAAGACATGAAGGTGGCGATCATCCCTGCCAT‑CACA                                                                        >  SRR4302535.3029105/1‑94 (MQ=60)
                     ACCCGCCAGGTTCATATACACCGGGCGAATATACGTGAAGAAAGCAAACTGCCCGGCGAAAGACATGAAGGTGGCGATCATCCCTGCCAT‑CACA                                                                        <  SRR4302535.317447/94‑1 (MQ=60)
                      CCCGCCAGGTTCATATACACCGGGCGAATATACGTGAAGAAAGCAAACTGCCCGGCGAAAGACATGAAGGTGGCGATCATCCCTGCCAT‑CACAC                                                                       >  SRR4302535.1506013/1‑94 (MQ=60)
                      CCCGCCAGGTTCATATACACCGGGCGAATATACGTGAAGAAAGCAAACTGCCCGGCGAAAGACATGAAGGTGGCGATAATCACTGCCAT‑CACAC                                                                       >  SRR4302535.67187/1‑94 (MQ=60)
                        CGCCAGGTTCATATACACCGGGCGAATATACGTGAAGAAAGCAAACTGCCCGGCGAAAGACATGAAGGTGGCGATCATCCCTGCCAT‑CACACCC                                                                     <  SRR4302535.3424287/94‑1 (MQ=60)
                         GCCAGGTTCATATACACCGGGCGAATATACGTGAAGAAAGCAAACTGCCCGGCGAAAGACATGAAGGTGGCGATCATCCCTGCCAT‑CACACCCG                                                                    >  SRR4302535.3334458/1‑94 (MQ=60)
                         GCCAGGTTCATATACACCGGGCGAATATACGTGAAGAAAGCAAACTGCCCGGCGAAAGACATGAAGATGGCGATCATCCCTGCCAT‑CACACCCG                                                                    >  SRR4302535.1965374/1‑94 (MQ=60)
                          CCAGGTTCATATACACCGGGCGAATATACGTGAAGAAAGCAAACTGCCCGGCGAAAGACATGAAGGTGGCGATCATCCCTGCCAT‑CACACCCGG                                                                   >  SRR4302535.4122821/1‑94 (MQ=60)
                          CCAGGTTCATATACACCGGGCGAATATACGTGAAGAAAGCAAACTGCCCGGCGAAAGACATGAAGGTGGCGATCATCCCTGCCAT‑CACACCCGG                                                                   >  SRR4302535.4553396/1‑94 (MQ=60)
                           CAGGTTCATATACACCGGGCGAATATACGTGAAGAAAGCAAACTGCCCGGCGAAAGACATGAAGGTGGCGATCATCCCTGCCAT‑CACACCCGGA                                                                  >  SRR4302535.1714576/1‑94 (MQ=60)
                            AGGTTCATATACACCGGGCGAATATACGTGAAGAAAGCAAACTGCCCGGCGAAAGACATGAAGGTGGCGATCATCCCTGCCAT‑CACACCCGGAC                                                                 <  SRR4302535.899251/94‑1 (MQ=60)
                            AGGTTCATATACACCGGGCGAATATACGTGAAGAAAGCAAACTGCCCGGCGAAAGACATGAAGGTGGCGATCATCCCTGCCAT‑CACACCCGGAC                                                                 <  SRR4302535.1218193/94‑1 (MQ=60)
                            AGGTTCATATACACCGGGCGAATATACGTGAAGAAAGCAAACTGCCCGGCGAAAGACATGAAGGTGGCGATCATCCCTGCCAT‑CACACCCGGAC                                                                 <  SRR4302535.2995711/94‑1 (MQ=60)
                            AGGTTCATATACACCGGGCGAATATACGTGAAGAAAGCAAACTGCCCGGCGAAAGACATGAAGGTGGCGATCATCCCTGCCAT‑CACACCCGGAC                                                                 <  SRR4302535.1632515/94‑1 (MQ=60)
                            AGGTTCATATACACCGGGCGAATATACGTGAAGAAAGCAAACTGCCAGGCGAAAGACATGAAGGTGGCGATCATCCCTGCCAT‑CACACCCGGAC                                                                 <  SRR4302535.3144332/94‑1 (MQ=60)
                             GGTTCATATACACCGGGCGAATATACGTGAAGAAAGCAAACTGCCCGGCGAAAGACATGAAGGTGGCGATCATCCCTGCCAT‑CACACCCGGACG                                                                <  SRR4302535.1668431/94‑1 (MQ=60)
                              GTTCATATACACCGGGCGAATATACGTGAAGAAAGCAAACTGCCCGGCGAAAGACATGAAGGTGGCGATCATCCCTGCCAT‑CACACCCGGACGT                                                               <  SRR4302535.907175/94‑1 (MQ=60)
                               TTCATATACACCGGGCGAATATACGTGAAGAAAGCAAACTGCCCGGCGAAAGACATGAAGGTGGCGATCATCCCTGCCAT‑CACACCCGGACGTT                                                              <  SRR4302535.890318/94‑1 (MQ=60)
                               TTCATATACACCGGGCGAATATACGTGAAGAAAGCAAACTGCCCGGCGAAAGACATGAAGGTGGCGATCATCCCTGCCAT‑CACACCCGGACGTT                                                              <  SRR4302535.688926/94‑1 (MQ=60)
                                TCATATACACCGGGCGAATATACGTGAAGAAAGCAAACTGCCCGGCGAAAGACATGAAGGTGGCGATCATCCCTGCCAT‑CACACCCGGACGTTG                                                             >  SRR4302535.706255/1‑94 (MQ=60)
                                TCATATACACCGGGCGAATATACGTGAAGAAAGCAAACTGCCCGGCGAAAGACATGAAGGTGGCGATCATCCCTGCCAT‑CACACCCGGACGTTG                                                             <  SRR4302535.604733/94‑1 (MQ=60)
                                TCATATACACCGGGCGAATATACGTGAAGAAAGCAAACTGCCCGGCGAAAGACATGAAGGTGGCGATCATCCCTGCCAT‑CACACCCGGACGTTG                                                             <  SRR4302535.542010/94‑1 (MQ=60)
                                    ATACACCGGGCGAATATACGTGAAGAAAGCAAACTGCCCGGCGAAAGACATGAAGGTGGCGATCATCCCTGCCAT‑CACACCCGGACGTTGTAAT                                                         >  SRR4302535.1609720/1‑94 (MQ=60)
                                    ATACACCGGGCGAATATACGTGAAGAAAGCAAACTGCCCGGCGAAAGACATGAAGGTGGCGATCATCCCTGCCAT‑CACACCCGGACGTTGTAAT                                                         <  SRR4302535.3156084/94‑1 (MQ=60)
                                      ACACCGGGCGAATATACGTGAAGAAAGCAAACTGCCCGGCGAAAGACATGAAGGTGGCGATCATCCCTGCCAT‑CACACCCGGACGTTGTAATAA                                                       <  SRR4302535.642185/94‑1 (MQ=60)
                                      ACACCGGGCGAATATACGTGAAGAAAGCAAACTGCCCGGCGAAAGACATGAAGGTGGCGATCATCCCTGCCAT‑CACACCCGGACGTTGTAATAA                                                       <  SRR4302535.1691324/94‑1 (MQ=60)
                                       CACCGGGCGAATATACGTGAAGAAATCAAACTGCCCGGCGAAAGACATGAAGGGGGCGATCATCCCTGCCAT‑CACACCCGGACGTTGTAATAAG                                                      <  SRR4302535.2308777/94‑1 (MQ=60)
                                       CACCGGGCGAATATACGTGAAGAAAGCAAACTGCCCGGCGAAGGACATGAAGGTGGCGATCATCCCTGCCAT‑CACACCCAGACGTTGTAATAAG                                                      >  SRR4302535.4321730/1‑94 (MQ=60)
                                       CACCGGGCGAATATACGTGAAGAAAGCAAACTGCCCGGCGAAAGACATGAAGGTGGCGATCATCCCTGCCAT‑CACACCCGGACGTTGTAATAAG                                                      <  SRR4302535.3645909/94‑1 (MQ=60)
                                        ACCGGGCGAATATACGTGAAGAAAGCAAACTGCCCGGCGAAAGACATGAAGGTGGCGATCATCCCTGCCAT‑CACACCCGGACGTTGTAATAAGC                                                     >  SRR4302535.18005/1‑94 (MQ=60)
                                         CCGGGCGAATATACGTGAAGAAAGCAAACTGCCCGGCGAAAGACATGAAGGTGGCGATCATCCCTGCCAT‑CACACCCGGACGTTGTAATAAGCG                                                    <  SRR4302535.2586572/94‑1 (MQ=60)
                                         CCGGGCGAATATACGTGAAGAAAGCAAACTGCCCGGCGAAAGACATGAAGGTGGCGATCATCCCTGCCAT‑CACACCCGGACGTTGTAATAAGCG                                                    <  SRR4302535.2583030/94‑1 (MQ=60)
                                           GGGCGAATATACGTGAAGAAAGCAAACTGCCCGGCGAAAGACATGAAGGTGGCGATCATCCCTGCCAT‑CACACCCGGACGTTGTAATAAGCGGA                                                  <  SRR4302535.1666078/94‑1 (MQ=60)
                                            GGCGAATATACGTGAAGAAAGCAAACTGCCCGGCGAAAGACATGAAGGTGGCGATCATCCCTGCCAT‑CACACCCGGACGTTGTAATAAGCGGAA                                                 <  SRR4302535.508580/94‑1 (MQ=60)
                                            GGCGAATATACGTGAAGAAAGCAAACTGCCCGGCGAAAGACATGAAGGTGGCGATCATCCCTGCCAT‑CACACCCGGACGTTGTAATAAGCGGAA                                                 <  SRR4302535.4654542/94‑1 (MQ=60)
                                             GCGAATATACGTGAAGAAAGCAAACTGCCCGGCGAAAGACATGAAGGTGGCGATCATCCCTGACAT‑CACACCCGGACGTTGTAATAAGCGGAAA                                                >  SRR4302535.309653/1‑94 (MQ=60)
                                              CGAATATACGTGAAGAAAGCAAACTGCCCGGCGAAAGACATGAAGGTGGCGATCATCCCTGCCAT‑CACACCCGGACGTTGTAATAAGCGGAAAG                                               <  SRR4302535.2121621/94‑1 (MQ=60)
                                              CGAATATACGTGAAGAAAGCAAACTGCCCGGCGAAAGACATGAAGGTGGCGATCATCCCTGCCAT‑CACACCCGGACGTTGTAATAAGCGGAAAG                                               <  SRR4302535.2108665/94‑1 (MQ=60)
                                              CGAATATACGTGAAGAAAGCAAACTGCCCGGCGAAAGACATGAAGGTGGCGATCATCCCTGCCAT‑CACACCCGGACGTTGTAATAAGCGGAAAG                                               <  SRR4302535.2528377/94‑1 (MQ=60)
                                              CGAATATACGTGAAGAAAGCAAACTGCCCGGCGAAAGACATGAAGGTGGCGATCATCCCTGCCAT‑CACACCCGGACGTTGTAATAAGCGGAAAG                                               <  SRR4302535.1303064/94‑1 (MQ=60)
                                               GAATATACGTGAAGAAAGCAAACTGCCCGGCGAAAGACATGAAGGTGGCGATCATCCCTGCCAT‑CACACCCGGACGTTGTAATAAGCGGAAAGT                                              <  SRR4302535.2076559/94‑1 (MQ=60)
                                               GAATATACGTGAAGAAAGCAAACTGCCCGGCGAAAGACATGAAGGTGGCGATCATCCCTGCCAT‑CACACCCGGACGTTGTAATAAGCGGAAAGT                                              <  SRR4302535.250432/94‑1 (MQ=60)
                                                AATATACGTGAAGAAAGCAAACTGCCCGGCGAAAGACATGAAGGTGGCGATCATCCCTGCCAT‑CACACCCGGACGTTGTAATAAGCGGAAAGTA                                             >  SRR4302535.1258263/1‑94 (MQ=60)
                                                AATATACGTGAAGAAAGCAAACTGCCCGGCGAAAGACATGAAGGTGGCGATCATCCCTGCCAT‑CACACCCGGACGTTGTAATAAGCGGAAAGTA                                             >  SRR4302535.1926642/1‑94 (MQ=60)
                                                AATATACGTGAAGAAAGCAAACTGCCCGGCGAAAGACATGAAGGTGGCGATCATCCCTGCCAT‑CACACCCGGACGTTGTAATAAGCGGAAAGTA                                             >  SRR4302535.2741036/1‑94 (MQ=60)
                                                AATATACGTGAAGAAAGCAAACTGCCCGGCGAAAGACATGAAGGTGGCGATCATCCCTGCCAT‑CACACCCGGACGTTGTAATAAGCGGAAAGTA                                             >  SRR4302535.2233979/1‑94 (MQ=60)
                                                 ATATACGTGAAGAAAGCAAACTGCCCGGCGAAAGACATGAAGGTGGCGATCATCCCTGCCAT‑CACACCCGGACGTTGTAATAAGCGGAAAGTAT                                            <  SRR4302535.2781056/94‑1 (MQ=60)
                                                 ATATACGTGAAGAAAGCAAACTGCCCGGCGAAAGACATGAAGGTGGCGATCATCCCTGCCAT‑CACACCCGGACGTTGTAATAAGCGGAAAGTAT                                            <  SRR4302535.4370781/94‑1 (MQ=60)
                                                  TATACGTGAAGAAAGCAAACTGCCCGGCGAAAGACATGAAGGTGGCGATCATGCCTGCCAT‑CACACCCGGACGTTGTAATAAGCGGAAAGTATT                                           <  SRR4302535.1957833/94‑1 (MQ=60)
                                                   ATACGTGAAGAAAGCAAACTGCCCGGCGAAAGACATGAAGGTGGCGATCATCCCTGCCAT‑CACACCCGGACGTTGTAATAAGCGGAAAGTATTT                                          >  SRR4302535.699023/1‑94 (MQ=60)
                                                    TACGTGAAGAAAGCAAACTGCCCGGCGAAAGACATGAAGGTGGCGATCATCCCTGCCAT‑CACACCCGGACGTTGTAATAAGCGGAAAGTATTTT                                         <  SRR4302535.1630549/94‑1 (MQ=60)
                                                    TACGTGAAGAAAGCAAACTGCCCGGCGAAAGACATGAAGGTGGCGATCATCCCTGCCAT‑CACACCCGGACGTTGTAATAAGCGGAAAGTATTTT                                         <  SRR4302535.2138448/94‑1 (MQ=60)
                                                     ACGTGAAGAAAGCAAACTTCCCGGCGAAAGACATGAAGGTGGCGATCATCCCTGCCAT‑CACACCCGGACGTTGTAATAAGCGGAAAGTATTTTG                                        <  SRR4302535.1154764/94‑1 (MQ=60)
                                                     ACGTGAAGAAAGCAAACTGCCCGGCGAAAGCCATGAAGGGGGCGACCATCCCTGCCATACAC‑CCCGGCCGTTGTAATAAGCGGAAAGTATTTTG                                        >  SRR4302535.4431846/1‑94 (MQ=60)
                                                     ACGTGAAGAAAGCAAACTGCCCGGCGAAAGACATGAAGGTGGCGATCATCCCTGCCAT‑CACACCCGGACGTTGTAATAAGCGGAAAGTATTTTG                                        <  SRR4302535.4118745/94‑1 (MQ=60)
                                                      CGTGAAGAAAGCAAACTGCCCGGCGAAAGACATGAAGGTGGCGATCATCCCTGCCAT‑CACACCCGGACGTTGTAATAAGCGGAAAGTATTTTGT                                       >  SRR4302535.1912315/1‑94 (MQ=60)
                                                      CGTGAAGAAAGCAAACTGCCCGGCGAAAGACATGAAGGTGGCGATCATCCCTGCCAT‑CACACCCGGACGTTGTAATAAGCGGAAAGTATTTTGT                                       <  SRR4302535.3582641/94‑1 (MQ=60)
                                                      CGTGAAGAAAGCAAACTGCCCGGCGAAAGACATGAAGGTGGCGATCATCCCTGCCAT‑CACACCCGGACGTTGTAATAAGCGGAAAGTATTTTGT                                       <  SRR4302535.4057941/94‑1 (MQ=60)
                                                      CGTGAAGAAAGCAAACTGCCCGGCGAAAGACATGAAGGTGGCGATCATCCATGCCAT‑CACACCCGGACGTTGTAATAAGCGGAAAGTATTTTGT                                       <  SRR4302535.3331590/94‑1 (MQ=60)
                                                       GTGAAGAAAGCAAACTGCCCGGCGAAAGACATGAAGGTGGCGATCATCCCTGCCAT‑CACACCCGGACGTTGTAATAAGCGGAAAGTATTTTGTT                                      >  SRR4302535.3626542/1‑94 (MQ=60)
                                                        TGAAGAAAGCAAACTGCCCGGCGAAAGACATGAAGGTGGCGATCATCCCTGCCAT‑CACACCCGGACGTTGTAATAAGCGGAAAGTATTTTGTTT                                     >  SRR4302535.4352369/1‑94 (MQ=60)
                                                        TGAAGAAAGCAAACTGCCCGGCGAAAGACATGAAGGTGGCGATCATCCCTGCCAT‑CACACCCGGACGTTGTAATAAGCGGAAAGTATTTTGTTT                                     >  SRR4302535.708753/1‑94 (MQ=60)
                                                         GAAGAAAGCAAACTGCCCGGCGAAAGACATGAAGGTGGCGATCATCCCTGCCAT‑CACACCCGGACGTTGTAATAAGCGGAAAGTATTTTGTTTC                                    <  SRR4302535.3678764/94‑1 (MQ=60)
                                                          AGGAAAGCAAACTGCCCGGCGAAAGACATGAAGGTGTCGATCATCCCTGCCAT‑CACACCCGGACGTTGTAATAAGCGGAAAGTATTTTGTTTCT                                   <  SRR4302535.18978/94‑1 (MQ=60)
                                                          AAGAAAGCAAACTGCCCGGCGAAAGACATGAAGGTGGCGATCATCCCTGCCAT‑CACACCCGGACGTTGTAATAAGCGGAAAGTATTTTGTTTCT                                   <  SRR4302535.4246535/94‑1 (MQ=60)
                                                          AAGAAAGCAAACTGCCCGGCGAAAGACATGAAGGTGGCGATCATCCCTGCCAT‑CACACCCGGACGTTGTAATAAGCGGAAAGTATTTTGTTTCT                                   >  SRR4302535.2221966/1‑94 (MQ=60)
                                                           AGAAAGCAAACTGCCCGGCGAAAGACATGAAGGTGGCGATCATCCCTGCCAT‑CACACCCGGACGTTGTAATAAGCGGAAAGTATTTTGTTTCTG                                  >  SRR4302535.4675601/1‑94 (MQ=60)
                                                           AGAAAGCAAACTGCCCGGCGAAAGACATGAAGGTGGCGATCATCCCTGCCAT‑CACACCCGGACGTTGTAATAAGCGGAAAGTATTTTGTTTCTG                                  >  SRR4302535.2023166/1‑94 (MQ=60)
                                                           AGAAAGCAAACTGCCCGGCGAAAGACATGAAGGTGGCGATCATCCCTGCCAT‑CACACCCGGACGTTGTAATAAGCGGAAAGTATTTTGTTTCTG                                  >  SRR4302535.1477729/1‑94 (MQ=60)
                                                             AAAGCAAACTGCCCGGCGAAAGACATGAAGGTGGCGATCATCCCTGCCAT‑CACACCCGGACGTTGTAATAAGCGGAAAGTATTTTGTTTCTGAT                                >  SRR4302535.664401/1‑94 (MQ=60)
                                                             AAAGCAAACTGCCCGGCGAAAGACATGAAGGTGGCGATCATCCCTGCCAT‑CACACCCGGACGTTGTAATAAGCGGAAAGTATTTTGTTTCTGAT                                <  SRR4302535.2910918/94‑1 (MQ=60)
                                                             AAAGCAAACTGCCCGGCGAAAGACATGAAGGTGGCGATCATCCCTGCCAT‑CACACCCGGACGTTGTAATAAGCGGAAAGTATTTTGTTTCTGAT                                <  SRR4302535.47097/94‑1 (MQ=60)
                                                              AAGCAAACTGCCCGGCGAAAGACATGAAGGTGGCGATCATCCCTGCCAT‑CACACCCGGACGTTGTAATAAGCGGAAAGTATTTTGTTTCTGATG                               <  SRR4302535.4221499/94‑1 (MQ=60)
                                                               AGCAAACTGCCCGGCGAAAGACATGAAGGTGGCGATCATCCCTGCCAT‑CACACCCGGACGTTGTAATAAGCGGAAAGTATTTTGTTTCTGATGC                              >  SRR4302535.1632232/1‑94 (MQ=60)
                                                                acaaacgggCCGGCGAAAAACATGAAGGTGGCCATCATCCCTGCCAT‑CACACCCGGACGTTGTAATAAGCTGAAAGTATTTTGTTTCTGATGCG                             <  SRR4302535.2010161/85‑1 (MQ=60)
                                                                GCAAACTGCCCGGCGAAAGACATGAAGGTGGCGATCATCCCTGCCAT‑CACACCCGGACGTTGTAATAAGCGGAAAGTATTTTGTTTCTGATGCG                             >  SRR4302535.453089/1‑94 (MQ=60)
                                                                 CAAACTGCCCGGCGAAAGACATGAAGGTGGCGATCATCCCTGCCAT‑CACACCCGGACGTTGTAATAAGCGGAAAGTATTTTGTTTCTGATGCGA                            <  SRR4302535.4075631/94‑1 (MQ=60)
                                                                  AAACTGCCCGGCGAAAGACATGAAGGTGGCGATCATCCCTGCCAT‑CACACCCGGACGTTGTAATAAGCGGAAAGTATTTTGTTTCTGATGCGAG                           <  SRR4302535.347082/94‑1 (MQ=60)
                                                                   AACTGCCCGGCGAAAGACATGAAGGTGGCGATCATCCCTGCCAT‑CACACCCGGACGTTGTAATAAGCGGAAAGTATTTTGTTTCTGATGCGAGG                          >  SRR4302535.4517889/1‑94 (MQ=60)
                                                                    ACTGCCCGGCGAAAGACATGAAGGTGGCGATCATCCCTGCCAT‑CACACCCGGACGTTGTAATAAGCGGAAAGTATTTTGTTTCTGATGCGAGGG                         <  SRR4302535.4294494/94‑1 (MQ=60)
                                                                     CTGCCCGGCGAAAGACATGAAGGTGGCGATCATCCCTGCCAT‑CACACCCGGACGTTGTAATAAGCGGAAAGTATTTTGTTTCTGATGCGAGGGT                        <  SRR4302535.437649/94‑1 (MQ=60)
                                                                      TGCCCGGCGAAAGACATGAAGGTGGCGATCATCCCTGCCAT‑CACACCCGGACGTTGTAATAAGCGGAAAGTATTTTGTTTCTGATGCGAGGGTT                       >  SRR4302535.1024823/1‑94 (MQ=60)
                                                                      TGCCCGGCGAAAGACATGAAGGTGGCGATCATCCCTGCCAT‑CACACCCGGACGTTGTAATAAGCGGAAAGTATTTTGTTTCTGATGCGAGGGTT                       <  SRR4302535.4665976/94‑1 (MQ=60)
                                                                       GCCCGGCGAAAGACATGAAGGTGGCGATCATCCCTGCCAT‑CACACCCGGACGTTGTAATAAGCGGAAAGTATTTTGTTTCTGATGCGAGGGTTC                      <  SRR4302535.2127296/94‑1 (MQ=60)
                                                                       GCCCGGCGAAAGACATGAAGGTGGCGATCATCCCTGCCAT‑CACACCCGGACGTTGTAATAAGCGGAAAGTATTTTGTTTCTGATGCGAGGGTTC                      >  SRR4302535.3494535/1‑94 (MQ=60)
                                                                        CCCGGCGAAAGACATGAAGGTGGCGATCATCCCTGCCAT‑CACACCCGGACGTTGTAATAAGCGGAAAGTATTTTGTTTCTGATGCGAGGGTTCG                     <  SRR4302535.1837731/94‑1 (MQ=60)
                                                                        CCCGGCGAAAGACATGAAGGTGGCGATCATCCCTGCCAT‑CACACCCGGACGTTGTAATAAGCGGAAAGTATTTTGTTTCTGATGCGAGGGTTCG                     <  SRR4302535.3945561/94‑1 (MQ=60)
                                                                        CCCGGCGAAAGACATGAAGGTGGCGATCATCCCTGCCAT‑CACACCCGGACGTTGTAATAAGCGGAAAGTATTTTGTTTCTGATGCGAGGGTTCG                     <  SRR4302535.2949721/94‑1 (MQ=60)
                                                                           GGCGAAAGACATGAAGGTGGCGATCATCCCTGCCAT‑CACACCCGGACGTTGTAATAAGCGGAAAGTATTTTGTTTCTGATGCGAGGGTTCGCCT                  >  SRR4302535.4208641/1‑94 (MQ=60)
                                                                           GGCGAAAGACATGAAGGTGGCGATCATCCCTGCCAT‑CACACCCGGACGTTGTAATAAGCGGAAAGTATTTTGTTTCTGATGCGAGGGTTCGCCT                  <  SRR4302535.2058890/94‑1 (MQ=60)
                                                                             CGAAAGACATGAAGGTGGCGATCATCCCTGCCAT‑CACGCCCGGACGTTGTAATAAGCGGAAAGTATTTTGTTTCTGATGCGAGGGTTCGCCTGG                <  SRR4302535.553017/94‑1 (MQ=60)
                                                                             CGAAAGACATGAAGGTGGCGATCATCCCTGCCAT‑CACACCCGGACGTTGTAATAAGCGGAAAGTATTTTGTTTCTGATGCGAGGGTTCGCCTGG                <  SRR4302535.3236665/94‑1 (MQ=60)
                                                                             CGAAAGACATGAAGGTGGCGATCATCCCTGCCAT‑CACACCCGGACGTTGTAATAAGCGGAAAGTATTTTGTTTCTGATGCGAGGGTTCGCCTGG                >  SRR4302535.528722/1‑94 (MQ=60)
                                                                              GAAAGACATGAAGGTGGCGATCATCCCTGCCAT‑CACACCCGGACGTTGTAATAAGCGGAAAGTATTTTGTTTCTGATGCGAGGGTTCGCCTGGC               >  SRR4302535.4214820/1‑94 (MQ=60)
                                                                              GAAAGACATGAAGGTGGCGATCATCCCTGCCAT‑CACACCCGGACGTTGTAATAAGCGGAAAGTATTTTGTTTCTGATGCGAGGGTTCGCCTGGC               >  SRR4302535.1643449/1‑94 (MQ=60)
                                                                                AAGACATGAAGGTGGCGATCATCCCTGCCAT‑CACACCCGGACGTTGTAATAAGCGGAAAGTATTTTGTTTCTGATGCGAGGGTTCGCCTGGCAG             >  SRR4302535.4525751/1‑94 (MQ=60)
                                                                                 AGACATGAAGGTGGCGATCATCCCTGCCAT‑CACACCCGGGCGTTGTAATAAGCGGAAAGGATTTTGTTTCTGATGCGAGGGgtccgctgggagt            >  SRR4302535.1812603/1‑81 (MQ=60)
                                                                                 AGACATGAAGGTGGCGATCATCCCTGCCAT‑CACACCCGGACGTTGTAATAAGCGGAAAGTATTTTGTTTCTGATGCGAGGGTTCGCCTGGCAGT            >  SRR4302535.1463567/1‑94 (MQ=60)
                                                                                 AGACATGAAGGTGGCGATCATCCCTGCCAT‑CACACCCGGACGTTGTAATAAGCGGAAAGTATTTTGTTTCTGATGCGAGGGTTCGCCTGGCAGT            >  SRR4302535.2858555/1‑94 (MQ=60)
                                                                                 AGACATGAAGGTGGCGATCATCCCTGCCAT‑CACACCCGGACGTTGTAATAAGCGGAAAGTATTTTGTTTCTGATGCGAGGGTTCGCCTGGCAGT            <  SRR4302535.281840/94‑1 (MQ=60)
                                                                                 AGACATGAAGGTGGCGATCATCCCTGCCAT‑CACACCCGGACGTTGTAATAAGCGGAAAGTATTTTGTTTCTGATGCGAGGGTTCGCCTGGCAGT            <  SRR4302535.2086693/94‑1 (MQ=60)
                                                                                   ACATGAAGGTGGCGATCATCCCTGCCAT‑CACACCCGGACGTTGTAATAAGCGGAAAGTATTTTGTTTCTGATGCGAGGGTTCGCCTGGCAGTGA          >  SRR4302535.276965/1‑94 (MQ=60)
                                                                                    CATGAAGGTGGCGATCATCCCTGCCAT‑CACACCCGGACGTTGTAATAAGCGGAAAGTATTTTGTTTCTGATGCGAGGGTTCGCCTGGCAGTGAA         >  SRR4302535.1284821/1‑94 (MQ=60)
                                                                                     ATGAAGGTGGCGATCATCCCTGCCAT‑CACACCCGGACGTTGTAATAAGCGGAAAGTATTTTGTTTCTGATGCGAGGGTTCGCCTGGCAGTGGAG        >  SRR4302535.426469/1‑94 (MQ=60)
                                                                                     ATGAAGGTGGCGATCATCCCTGCCAT‑CACACCCGGACGTTGTAATAAGCGGAAAGTATTTTGTTTCTGATGCGAGGGTTCGCCTGGCAGTGAAG        >  SRR4302535.93614/1‑94 (MQ=60)
                                                                                     ATGAAGGTGGCGATCATCCCTGCCAT‑CACACCCGGACGTTGTAATAAGCGGAAAGTATTTTGTTTCTGATGCGAGGGTTCGCCTGGCAGTGAAG        >  SRR4302535.513849/1‑94 (MQ=60)
                                                                                      TGAAGGTGGCGATCATCCCTGCCAT‑CACACCCGGACGTTGTAATAAGCGGAAAGTATTTTGTTTCTGATGCGAGGGTTCGCCTGGCAGTGAAGG       <  SRR4302535.4183591/94‑1 (MQ=60)
                                                                                       GAAGGTGGCGATCATCCCTGCCAT‑CACACCCGGACGTTGTAATAAGCGGAAAGTATTTTGTTTCTGATGCGAGGGTTCGCCTGGCAGTGAAGGC      <  SRR4302535.2211735/94‑1 (MQ=60)
                                                                                        AAGGTGGCGATCATCCCTGCCAT‑CACACCCGGACGTTGTAATAAGCGGAAAGTATTTTGTTTCTGATGCGAGGGTTCGCCTGGCAGTGAAGGCA     <  SRR4302535.3343897/94‑1 (MQ=60)
                                                                                         AGGTGGCGATCATCCCTGCCAT‑CACACCCGGACGTTGTAATAAGCGGAAAGTATTTTGTTTCTGATGCGAGGGTTCGCCTGGCAGTGAAGGCAA    >  SRR4302535.4239390/1‑94 (MQ=60)
                                                                                          GGTGGCGATCATCCCTGCCAT‑CACACCCGGACGTTGTAATAAGCGGAAAGTATTTTGTTTCTGATGCGAGGGTTCGCCTGGCAGTGAAGGCAAT   <  SRR4302535.3237621/94‑1 (MQ=60)
                                                                                           GTGGCGATCATCCCTGCCAT‑CACACCCGGACGTTGTAATAAGCGGAAAGTATTTTGTTTCTGATGCGAGGGTTCGCCTGGCAGTGAAGGCAATG  <  SRR4302535.2117508/94‑1 (MQ=60)
                                                                                           |                                                                                              
CGTTAAGCCATCCACGCCGAATCCCGCCAGGTTCATATACACCGGGCGAATATACGTGAAGAAAGCAAACTGCCCGGCGAAAGACATGAAGATGGCGATCATCCCTGCCAT‑CACACCCGGACGTTGTAATAAGCGGAAAGTATTTTGTTTCTGATGCGAGGGTTCGCCTGGCAGTGAAGGCAATG  >  NC_000913/3838602‑3838786

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑