Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 1873 183.1 9376148 98.2% 9207377 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,847,265 A→G W167R (TGG→CGG)  b3668 ← sensor histidine protein kinase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,847,2650AG99.5% 659.8 / ‑4.9 214W167R (TGG→CGG) b3668sensor histidine protein kinase
Reads supporting (aligned to +/- strand):  ref base A (1/0);  new base G (82/131);  total (83/131)
Fisher's exact test for biased strand distribution p-value = 3.88e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.79e-01

Only 100 of 239 total aligned reads displayed.

GACTGATAACAAACAGCAGCAAGTACCAGACCAGATGTCGCCCGCGCCAGTTGATTGGCTGAGAAACCAGTGACGGACCGAGCGGCAGCCAGGTGTTATTGGCGAGATAGTGCCAGAACACCAGACATATCGGGGCCAGCGTCAGGCCGCCAGTTAAAGTCAGCAACAGCGCATTCCACG  >  NC_000913/3847175‑3847354
                                                                                          |                                                                                         
 aCTGATAACAAACAGCAGCAAGTACCAGACCAGATGTCGCCCGCGCCAGTTGATTGGCTGAGAAACCAGTGACGGACCGAGCGGCAGCCGGgt                                                                                        <  1:676988/93‑1 (MQ=255)
  cTGATAACAAACAGCAGCAAGTACCAGACCAGATGTCGCCCGCGCCAGTTGATTGGCTGAGAAACCAGTGACGGACCGAGCGGCAGCCgggat                                                                                       >  1:4038020/1‑91 (MQ=255)
  cTGATAACAAACAGCAGCAAGTACCAGACCAGATGTCGCCCGCGCCAGTTGATTGGCTGAGAAACCAGTGACGGACCGAGCGGCAGCCGGgtg                                                                                       >  1:4227803/1‑93 (MQ=255)
   tGATAACAAACAGCAGCAAGTACCAGACCAGATGTCGCCCGCGCCAGTTGATTGGCTGAGAAACCAGTGACGGACCGAGCGGCAGCCGGgtgt                                                                                      >  2:3231272/1‑93 (MQ=255)
   tGATAACAAACAGCAGCAAGTACCAGACCAGATGTCGCCCGCGCCAGTTGATTGGCTGAGAAACCAGTGACGGACCGAGCGGCAGCCGGgtgt                                                                                      <  2:2510818/93‑1 (MQ=255)
   tGATAACAAACAGCAGCAAGTACCAGACCAGATGTCGCCCGCGCCAGTTGATTGGCTGAGAAACCAGTGACGGACCGAGCGGCAGCCGGgtgt                                                                                      <  1:2184968/93‑1 (MQ=255)
   tGATAACAAACAGCAGCAAGTACCAGACCAGATGTCGCCCGCGCCAGTTGATTGGCTGAGAAACCAGTGACGGACCGAGCGGCAGCCGGgtgt                                                                                      >  2:2147126/1‑93 (MQ=255)
   tGATAACAAACAGCAGCAAGTACCAGACCAGATGTCGCCCGCGCCAGTTGATTGGCTGAGAAACCAGTGACGGACCGAGCGGCAGCCGGgtgt                                                                                      >  1:1818402/1‑93 (MQ=255)
   tGATAACAAACAGCAGCAAGTACCAGACCAGATGTCGCCCGCGCCAGTTGATTGGCTGAGAAACCAGTGACGGACCGAGCGGCAGCCGGgtgt                                                                                      <  1:1762794/93‑1 (MQ=255)
   tGATAACAAACAGCAGCAAGTACCAGACCAGATGTCGCCCGCGCCAGTTGATTGGCTGACAAACCAGTGACGGACCGAGCGGCAGCCGGgggt                                                                                      >  1:2807180/1‑93 (MQ=255)
     aTAACAAACAGCAGCAAGTACCAGACCAGATGTCGCCCGCGCCAGTTGATTGTCTGAGAAACCAGTGACGGACCGAGCGGCAGCCGGGTGTTa                                                                                    <  1:812149/93‑1 (MQ=255)
     aTAACAAACAGCAGCAAGTACCAGACCAGATGTCGCCCGCGCCAGTTGATTGGCTGAGAAACCAGTGACGGACCGAGCGGCAGCCGGGTGTTa                                                                                    <  2:3137593/93‑1 (MQ=255)
     aTAACAAACAGCAGCAAGTACCAGACCAGATGTCGCCCGCGCCAGTTGATTGGCTGAGAAACCAGTGACGGACCGAGCGGCAGCCGGGTGTTa                                                                                    <  1:1188555/93‑1 (MQ=255)
      tatcaaacaGCAGCAAGTACCAGACCAGATGTCGCCCGCGCCAGTTGATTGGCTGAGAAACCAGTGACGGACCGAGCGGCAGCCGGGTGTTAt                                                                                   >  1:3780903/4‑93 (MQ=255)
       aacaaacaGCAGCAAGTACCAGACCAGATGTCGCCCGCGCCAGTTGATTGGCTGAGAAACCAGTGACGGACTGAGCGGCAGCCGGGCGTTAtt                                                                                  >  1:4590602/1‑93 (MQ=255)
       aacaaacaGCAGCAAGTACCAGACCAGATGTCGCCCGCGCCAGTTGATTGGCTGAGAAACCAGTGACGGACCGAGCGGCAGCCGGGTGTTAtt                                                                                  <  1:3741054/93‑1 (MQ=255)
       aacaaacaGCAGCAAGTACCAGACCAGATGTCGCCCGCGCCAGTTGATTGGCTGAGAAACCAGTGACGGACCGAGCGGCAGCCGGGTGTTAtt                                                                                  <  2:1311338/93‑1 (MQ=255)
        acaaacaGCAGCAAGTACCAGACCAGATGTCGCCCGCGCCAGTTGATTGGCTGAGAAACCAGTGACGGACCGAGCGGCAGCCGGGTGTTATTg                                                                                 <  2:3681452/93‑1 (MQ=255)
        acaaacaGCAGCAAGTACCAGACCAGATGTCGCCCGCGCCAGTTGATTGGCTGAGAAACCAGTGACGGACCGAGCGGCAGCCGGGTGTTATTg                                                                                 <  1:2999533/93‑1 (MQ=255)
         caaacaGCAGCAAGTACCAGACCAGATGTCGCCCGCGCCAGTTGATTGGCTGAGAAACCAGTGACGGACCGAGCGGCAGCCGGGTGTTATTgg                                                                                <  2:2755709/93‑1 (MQ=255)
           acgtgcagcaAGTGCCAGACCAGATGCCGCGCGCGCCAGTTGATTGGCTGAGAAACCAGTGACGGACCGAGCGGCAGCCGGGTGTTATTGGCg                                                                              <  1:3338549/89‑1 (MQ=255)
           aacaGCAGCAAGTACCAGACCAGATGTCGCCCGCGCCAGTTGATTGGCTGAGAAACCAGTGACGGACCGAGCGGCAGCCGGGTGTTATTGGCg                                                                              >  2:2373538/1‑93 (MQ=255)
           aacaGCAGCAAGTACCAGACCAGATGTCGCCCGCGCCAGTTGATTGGCTGAGAAACCAGTGACGGACCGAGCGGCAGCCGGGTGTTATTGGCg                                                                              >  1:1210556/1‑93 (MQ=255)
           aacaGCAGCAAGTACCAGACCAGATGTCGCCCGCGCCAGTTGATTGGCTGAGAAACCAGTGACGGACCGAGCGGCAGACGGGTGTTATTGGCg                                                                              >  1:10819/1‑93 (MQ=255)
           aacaGCAGCAAGTACCAGACCAGATGTCGCCCGCGCCAGTTGATTGGCTGAGAAACCAGTGACGGACCGAGCGGAAGCCGGGTGTTATTGTCg                                                                              <  2:1460097/93‑1 (MQ=255)
              agcagcAAGTACCAGACCAGATGTCGCCCGCGCCAGTTGATTGGCTGAGAAACCAGTGACGGACCGAGCGGCAGCCGGGTGTTATTGGCgaga                                                                           <  2:592637/93‑1 (MQ=255)
               gcagcaAGTACCAGACCAGATGTCGCCCGCGCCAGTTGATTGGCTGAGAAACCAGTGACGGACCGAGCGGCAGCCGGGTGTTATTGGCGAGAt                                                                          <  2:2745837/93‑1 (MQ=255)
               gcagcaAGTACCAGACCAGATGTCGCCCGCGCCAGTTGATTGGCTGAGAAACCAGTGACGGACCGAGCGGCAGCCGGGTGTTATTGGCGAGAt                                                                          >  1:1655214/1‑93 (MQ=255)
                 agcaAGTACCAGACCAGATGTCGCCCGCGCCAGTTGATTGGCTGAGAAACCAGTGACGGACCGAGCGGCAGCCGGGTGTTATTGGCGAGATAg                                                                        <  1:3271568/93‑1 (MQ=255)
                 agcaAGTACCAGACCAGATGTCGCCCGCGCCAGTTGATTGGCTGAGAAACCAGTGACGGACCGAGCGGCAGCCGGGTGTTATTGGCGAGATAg                                                                        >  2:1050702/1‑93 (MQ=255)
                 agcaAGTACCAGACCAGATGTCGCCCGCGCCAGTTGATTGGCTGAGAAACCAGTGACGGACCGAGCGGCAGCCGGGTGTTATTGGCGAGATAg                                                                        <  2:3738825/93‑1 (MQ=255)
                  gcaAGTACCAGACCAGATGTCGCCCGCGCCAGTTGATTGGCTGAGAAACCAGTGACGGACCGAGCGGCAGCCGGGTGTTATTGGCGAGATAgg                                                                       >  1:2146019/1‑92 (MQ=255)
                  gcaAGTACCAGACCAGATGTCGCCCGCGCCAGTTGATTGGCTGAGAAACCAGTGACGGACCGAGCGGCAGCCGGGTGTTATTGGCGAGATAGt                                                                       <  2:1825597/93‑1 (MQ=255)
                  gcaAGTACCAGACCAGATGTCGCCCGCGCCAGTTGATTGGCTGAGAAACCAGTGACGGACCGAGCGGCAGCCGGGTGTTATTGGCGAGATAGt                                                                       <  2:1973719/93‑1 (MQ=255)
                   caAGTACCAGACCAGATGTCGCCCGCGCCAGTTGATTGGCTGAGAAACCAGTGACGGACCGAGCGGCAGCCGGGTGTTATTGGCGAGATAGTg                                                                      >  2:449388/1‑93 (MQ=255)
                    aaGTACCAGACCAGATGTCGCCCGCGCCAGTTGATTGGCTGAGAAACCAGTGACGGACCGAGCGGCAGCCGGGGGTTATTGGCGAGATAGGGc                                                                     >  1:3976505/1‑93 (MQ=255)
                     aGTACCAGACCAGATGTCGCCCGCGCCAGTTGATTGGCTGAGAAACCAGTGACGGACCGAGCGGCAGCCGGGTGTTATTGGCGAGATAGTGcc                                                                    >  2:1502875/1‑93 (MQ=255)
                      gTACCAGACCAGATGTCGCCCGCGCCAGTTGATTGGCTGAGAAACCAGTGACGGACCGAGCGGCAGCCGGGTGTTATTGGCGAGATAGTGCCa                                                                   >  1:2538677/1‑93 (MQ=255)
                       tACCAGACCAGATGTCGCCCGCGCCAGTTGATTGGCTGAGAAACCAGTGACGGACCGAGCGGCAGCCGGGTGTTATTGGCGAGATAGTGCCAg                                                                  >  1:3197394/1‑93 (MQ=255)
                        accagaccagATGTCGCCCGCGCCAGTTGATTGGCTGAGAAACCAGTGACGGACCGAGCGGCAGCCGGGTGTTATTGGCGAGATAGTGCCAGa                                                                 >  2:392979/1‑93 (MQ=255)
                          cagaccagaTGTCGCCCGCGCCAGTTGATTGGCTGAGAAACCAGTGACGGACCGAGCGGCAGCCGGGTGTTATTGGCGAGATAGTGCCAGAac                                                               <  1:870521/93‑1 (MQ=255)
                           agaccagaTGTCGCCCGCGCCAGTTGATTGGCTGAGAAACCAGTGACGGACCGAGCGGCAGCCGGGTGTTATTGGCGAGATAGTGCCAGAaca                                                              >  2:2234919/1‑93 (MQ=255)
                           agaccagaTGTCGCCCGCGCCAGTTGATTGGCTGAGAAACCAGTGACGGACCGAGCGGCAGCCGGGTGTTATTGGCGAGATAGTGCCAGAaca                                                              <  1:1854288/93‑1 (MQ=255)
                           agaccagaTGTCGCCCGCGCCAGTTGATTGGCTGAGAAACCAGTGACGGACCGAGCGGCAGCCGGGTGTTATTGGCGAGATAGTGCCAGAaca                                                              <  1:4384282/93‑1 (MQ=255)
                           agaccagaTGTCGCCCGCGCCAGCTGATTGGCTGAGAAACCAGTGACGGACCGAGCGGCAGCCGGGGGTTATTGGCGATGAAGTGCCCGAaca                                                              >  2:3582748/1‑93 (MQ=255)
                            gaccagaTGTCGCCCGCGCCAGTTGATTGGCTGAGAAACCAGTGACGGACCGAGCGGCAGCCGGGTGTTATTGGCGAGATAGTGGCAGAacac                                                             >  1:849060/1‑93 (MQ=255)
                            gaccagaTGTCGCCCGCGCCAGTTGATTGGCTGAGAAACCAGTGACGGACCGAGCGGCAGCCGGGTGTTATTGGCGAGATAGTGCCAGAacac                                                             >  1:535794/1‑93 (MQ=255)
                             accagaTGTCGCCCGCGCCAGTTGATTGGCTGAGAAACCAGTGACGGACCGAGCGGCAGCCGGGTGTTATTGGCGAGATAGTGCCAGAACAcc                                                            >  1:1951725/1‑93 (MQ=255)
                               cagaTGTCGCCCGCGCCAGTTGATTGGCTGAGAAACCAGTGACGGACCGAGCGGCAGCCGGGTGTTATTGGCGAGATAGTGCCAGAACACCAg                                                          <  1:2578930/93‑1 (MQ=255)
                               cagaTGTCGCCCGCGCCAGTTGATTGGCTGAGAAACCAGGGACGGACCGAGGGGCAGCCGGGTGTTATTTGAGAGATAGTTCCAGAACACCaa                                                          >  2:20648/1‑92 (MQ=255)
                                cgacgTCGCCCGCGCCAGTTGATTGGCTGAGAAACCAGTGACGGACCGAGCGGCAGCCGGGTGTTATTGGCGAGATAGTGCCAGAACACCAGa                                                         <  1:14959/89‑1 (MQ=255)
                                 gaTGTCGCCCGCGCCAGTTGATTGGCTGGGAAACCAGTGACGGACCGAGCGGCAGCCGGGTGTTATTGGCGAGATAGTGCCAGAACACCAGAc                                                        <  2:3789755/93‑1 (MQ=255)
                                 gaTGTCGCCCGCGCCAGTTGATTGGCTGAGAAACCAGTGACGGACCGAGCGGCAGCCGGGTGTTATTGGCGAGATAGTGCCAGAACACCAGAc                                                        >  1:735057/1‑93 (MQ=255)
                                 gaTGTCGCCCGCGCCAGTTGATTGGCTGAGAAACCAGTGACGGACCGAGCGGCAGCCGGGTGTTATTGGCGAGATAGTGCCAGAACACCAGAc                                                        <  1:4351635/93‑1 (MQ=255)
                                 gaTGTCGCCCGCGCCAGTTGATTGGCTGAGAAACCAGTGACGGACCGAGCGGCAGCCGGGTGTTATTGGCGAGATAGTGCCAGAACACCAGAc                                                        >  2:3330352/1‑93 (MQ=255)
                                  aTGTCGCCCGCGCCAGTTGATTGGCTGAGAAACCAGTGACGGACCGAGCGGCAGCCGGGCGTTATTGGCGAGATAGTGCCAGAACACCAGACa                                                       >  2:3628965/1‑93 (MQ=255)
                                  aTGTCGCCCGCGCCAGTTGATTGGCTGAGAAACCAGTGACGGACCGAGCGGCAGCCGGGAGTTATTGGCGAGATAGTGCCAGAACACCAGACa                                                       >  2:767640/1‑93 (MQ=255)
                                    gTCGCCCGCGCCAGTTGATTGGCTGAGAAACCAGTGACGGACCGAGCGGCAGCCGGGTGTTATTGGCGAGATAGTGCCAGAACACCAGACata                                                     <  1:3723873/93‑1 (MQ=255)
                                       gCCCGCGCCAGTTGATTGGCTGAGAAACCAGTGACGGACCGCGCGGCAGCCGGGGGGTATTTGCGCGATAGTGCCAGAACACCAGACATATCg                                                  >  2:2312437/1‑93 (MQ=255)
                                       gCCCGCGCCAGTTGATTGGCTGAGAAACCAGTGACGGACCGAGCGGCAGCCGGGTGTTATTGGCGAGATAGTGCCAGAACACCAGACATATCg                                                  <  1:3085769/93‑1 (MQ=255)
                                       gCCCGCGCCAGTTGATTGGCTGAGAAACCAGTGACGGACCGAGCGGCAGCCGGGTGTTATTGGCGAGATAGTGCCAGAACACCAGACATATCg                                                  <  2:4193754/93‑1 (MQ=255)
                                         ccGCGCCAGTTGATTGGCTGAGAAACCAGTGACGGACCGAGCGGCAGCCGGGTGTTATTGGCGAGATAGTGCCAGAACACCAGACATATCggg                                                <  2:4553727/93‑1 (MQ=255)
                                         ccGCGCCAGTTGATTGGCTGAGAAACCAGTGACGGACCGAGCGGCAGCCGGGTGTTATTGGCGAGATAGTGCCAGAACACCAGACATATCggg                                                >  1:2684431/1‑93 (MQ=255)
                                         ccGCGCCAGTTGATTGGCTGAGAAACCAGTGACGGACCGAGCGGCAGCCGGGTGTTATTGGCGAGATAGTGCCAGAACACCAGACATATCggg                                                <  1:3588757/93‑1 (MQ=255)
                                         ccGCGCAAGTTGATTGGCTGAGAAACCAGTGACGTACCGAGCGTCAGCCGGGTGTTATTGGCGAGATAGTGCCAGAACGCCAGACATATCggg                                                <  2:2994987/93‑1 (MQ=255)
                                             gcCAGTTGATTGGCTGAGAAGCCAGTGACGGACCGAGCGGCAGCCGGGTGTTATTGGCGAGATAGTGCCAGAACACCAGACATATCGGGGCCa                                            <  2:42921/93‑1 (MQ=255)
                                              ccAGTTGATTGGCTGAGAAACCAGTGACGGACCGAGCGGCAGCCGGGTGTTATTGGCGAGATAGTGCCAGAACACCAGACATATCGGGGCCAg                                           <  2:2282171/93‑1 (MQ=255)
                                              ccAGTTGATTGGCTGAGAAACCAGTGACGGACCGAGCGGCAGCCGGGTGTTATTGGCGAGATAGTGCCAGAACACCAGACATATCGGGGCCAg                                           >  1:2886541/1‑93 (MQ=255)
                                                aGTTGATTGGCTGAGAAACCAGTGACGGACCGAGCGGCAGCCGGGTGTTATTGGCGAGATAGTGCCAGAACACCAGACAAATCGGGGCCGGCg                                         >  2:2597393/1‑93 (MQ=255)
                                                  ttGATTGGCTGAGAAACCAGTGACGGACCGAGCGGCAGCCGGGTGTTATTGGCGAGATAGTGCCAGAACACCAGACATATCGGGGTCAGCGTc                                       >  1:2042625/1‑93 (MQ=255)
                                                    gATTGGCTGAGAAACCAGTGACGGACCGAGCGGCAGCCGGGTGTTATTGGCGAGATAGTGCCAGAACACCAGACATATCGGGGCCAGCGTCAg                                     >  1:2455342/1‑93 (MQ=255)
                                                    gATTGGCTGAGAAACCAGTGACGGACCGAGCGGCAGCCGGGTGTTATTGGCGAGATAGTGCCAGAACACCAGACATATCGGGGCCAGCGTCAg                                     <  2:4462025/93‑1 (MQ=255)
                                                    gATTGGCTGAGAAACCAGTGACGGACCGAGCGGCAGCCGGGTGTTATTGGCGAGATAGGACCCAAACCCCAGACATATCGGGGCCAGCGTCAg                                     >  1:3522724/1‑93 (MQ=255)
                                                     aTTGGCTGAGAAACCAGTGACGGACCGAGCGGCAGCCGGGTGTTATTGGCGAGATAGTGCCAGAACACCAGACATATCGGGGCCAGCGTCAgg                                    >  1:2883249/1‑93 (MQ=255)
                                                      ttGGCTGAGAAACCAGTGACGGACCGAGCGGCAGCCGGGTGTTATTGGCGAGATAGTGCCAGAACACCAGACATATCGGGGCCAGCGTCAGgc                                   >  1:3846126/1‑93 (MQ=255)
                                                      ttGGCTGAGAAACCAGTGACGGACCGAGCGGCAGCCGGGTGTTATTGGCGAGATAGTGCCAGAACACCAGACATATCGGGGCCAGCGTCAGgc                                   >  2:2869624/1‑93 (MQ=255)
                                                       tGGCTGAGAAACCAGTGACGGACCGAGCGGCAGCCGGGGGTTATTGGCGAGATAGTGCCAGAACACCAGACATATCGGGGCCAGCGTCATgcc                                  >  1:4613922/1‑93 (MQ=255)
                                                          cTGAGAAACCAGTGACGGACCGAGCGGCAGCCGGGTGTTATTGGCGAGATAGTGCCAGAACACCAGACATATCGGGGCCAGCGTCAGgccgcc                               <  2:2339403/93‑1 (MQ=255)
                                                           tGAGAAACCAGTGACGGACCGAGCGGCAGCCGGGTGTTATTGGCGAGATAGTGCCAGAACTCCAGACATATCGGGGCCAGCGTCAGGCCGCCa                              >  2:4362070/1‑93 (MQ=255)
                                                            gagaAACCAGTGACGGACCGAGCGGCAGCCGGGTGTTATTGGCGAGATAGTGCCAGAACACCAGACATATCGGGGCCAGCGTCAGGCCGCCAg                             >  1:1061140/1‑93 (MQ=255)
                                                             agaAACCAGTGACGGACCGAGCGGCAGCCGGGTGTTATTGGCGAGATAGTGCCAGAACACCAGACATATCGGGGCCAGCGTCAGGCCGCCAGt                            <  2:2153954/93‑1 (MQ=255)
                                                               aaaCCAGTGACGGACCGAGCGGCAGCCGGGTGTTATTGGCGAGATAGTGCCAGAACACCAGACATATCGGGGCCAGCGTCAGGCCGCCAGTTa                          <  1:2697110/93‑1 (MQ=255)
                                                                 aCCAGTGACGGACCGAGCGGCAGCCGGGTGTTATTGGCGAGATAGTGCCAGAACACCAGACATATCGGGGCCAGCGTCAGGCCGCCAGTTaaa                        <  1:804505/93‑1 (MQ=255)
                                                                  ccAGTGACGGACCGAGCGGCAGCCGGGTGTTATTGGCGAGATAGTGCCAGAACACCAGACATATCGGGGCCAGCGTCAGGCCGCCAGTTAAAg                       <  2:1665525/93‑1 (MQ=255)
                                                                  ccAGTGACGGACCGAGCGGCAGCCGGGTGTTATTGGCGAGATAGTGCCAGAACACCAGACATATCGGGGCCAGCGTCAGGCCGCCAGTTAAAg                       <  2:1872772/93‑1 (MQ=255)
                                                                  ccAGTGACGGACCGAGCGGCAGCCGGGTGTTATTGGCGAGATAGTGCCAGAACACCAGACATATCGGGGCCAGCGTCAGGCCGCCAGTTAAAg                       >  2:1368319/1‑93 (MQ=255)
                                                                   cAGTGACGGACCGAGCGGCAGCCGGGTGTTATTGGCGAGATAGTGCCAGAACACCAGACATATCGGGGCCAGCGTCAGGCCGCCAGTTAAAGt                      <  1:1294281/93‑1 (MQ=255)
                                                                    aGTGACGGACCGAGCGGCAGCCGGGTGTTATTGGCGAGATAGTGCCAGAACACCAGACATATCGGGGCCAGCGTCAGGCCGCCAGTTAAAGTc                     <  2:3559090/93‑1 (MQ=255)
                                                                    aGTGACGGACCGAGCGGCAGCCGGGTGTTATTGGCGAGATAGTGCCAGAACACCAGACATATCGGGGCCAGCGTCAGGCCGCCAGTTAAAGTc                     <  1:1029588/93‑1 (MQ=255)
                                                                     gTGACGGACCGAGCGGCAGCCGGGTGTTATTGGCGAGATAGTGCCAGAACACCAGACATATCGGGGCCAGCGTCAGGCCGCCAGTTAAAGTCa                    >  1:4106511/1‑93 (MQ=255)
                                                                     gTGACGGACCGAGCGGCAGCCGGGTGTTATTGGCGAGATAGTGCCAGAACACCAGACATATCGGGGCCAGCGTCAGGCCGCCAGTTAAAGTCa                    <  1:999232/93‑1 (MQ=255)
                                                                           gACCGAGCGGCAGCCGGGTGTTATTGGCGAGATAGTGCCAGAACACCAGACATATCGGGGCCAGCGTCAGGCCGCCAGTTAAAGTCAGCAACa              <  1:3892144/93‑1 (MQ=255)
                                                                            aCCGAGCGCCACCCGGGTGTTATTGGCGAGATAGTGCCAGAACACCAGACATATCGGGGCCAGCGTCAGGCCGCCAGTTAAAGTCAGCAACAg             <  2:2565501/93‑1 (MQ=255)
                                                                              cGAGCGGCAGCCGGGTGTTATTGGCGAGATAGTGCCAGAACACCCGACATATCGGGGTCAGCGTCAGGCCGCCCGTTAAAGCAAGCAACAgcg           >  2:3796998/1‑93 (MQ=255)
                                                                               gcgCGGCAGCCGGGTGTTATTGGCGAGATAGTGCCAGAACACCAGACATATCGGGGCCAGCGTCAGGCCGCCAGTTAAAGTCAGCAACAgcgc          >  2:2835988/3‑93 (MQ=255)
                                                                               gAGCGGCAGCCGGGTGTTATTGGCGAGATAGTGCCAGAACACCAGACATATCGGGGCCAGCGTCAGGCCGCCAGTTAAAGTCAGCAACAgcgc          >  1:2324363/1‑93 (MQ=255)
                                                                                 gCGGCAGCCGGGTGTTATTGGCGAGATAGTGCCAGAACACCAGACATATCGGGGCCAGCGTCAGGCCGCCAGTTAAAGTCAGCAACAGCGCAt        >  2:2655332/1‑93 (MQ=255)
                                                                                    gCAGCCGGGTGTTATTGGCGAGATAGTGCCAGAACACCAGACATATCGGGGCCAGCGTCAGGCCGCCAGTTAAAGTCAGCAACAGCGCATTcc     <  2:2591155/93‑1 (MQ=255)
                                                                                    gCAGCCGGGTGTTATTGGCGAGATAGTGCCAGAACACCAGACATATCGGGGCCAGCGTCAGGCCGCCAGTTAAAGTCAGCAACAGCGCATTcc     <  2:4352444/93‑1 (MQ=255)
                                                                                      aGCCGGGTGTTATTGGCGAGATAGTGCCAGAACACCAGACATATCGGGGCCAGCGTCAGGCCGCCAGTTAAAGTCAGCAACAGCGCATTCCAc   <  2:161180/93‑1 (MQ=255)
                                                                                          |                                                                                         
GACTGATAACAAACAGCAGCAAGTACCAGACCAGATGTCGCCCGCGCCAGTTGATTGGCTGAGAAACCAGTGACGGACCGAGCGGCAGCCAGGTGTTATTGGCGAGATAGTGCCAGAACACCAGACATATCGGGGCCAGCGTCAGGCCGCCAGTTAAAGTCAGCAACAGCGCATTCCACG  >  NC_000913/3847175‑3847354

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
AGAGACTGATAACAAACAGCAGCAAGTACCAGACCAGATGTCGCCCGCGCCAGTTGATTGGCTGAGAAACCAGTGACGGACCGAGCGGCAGCCAGGTGTTATTGGCGAGATAGTGCCAGAACACCAGACATATCGGGGCCAGCGTCAGGCCGCCAGTTAAAGTCAGCAACAGCGCATTCCACGACTC  >  NC_000913/3847172‑3847358
                                                                                             |                                                                                             
AGAGACTGATAACAAACAGCAGCAAGTACCAGACCAGATGTCGCCCGCGCCAGTTGATTGGCTGAGAAACCAGTGACGGACCGAGCGGCAGCCG                                                                                               <  SRR4302535.1947385/94‑1 (MQ=60)
 GAGACTGATAACAAACAGCAGCAAGTACCAGACCAGATGTCGCCCGCGCCAGTTGATTGGCTGAGAAACCAGTGACGGACCGAGCGGCAGCCGG                                                                                              <  SRR4302535.4453877/94‑1 (MQ=60)
  AGACTGATAACAAACAGCAGCAAGTACCAGACCAGATGTCGCCCGCGCCAGTTGATTGGCTGAGAAACCAGTGACGGACCGAGCGGCAGCCGGG                                                                                             >  SRR4302535.2730952/1‑94 (MQ=60)
  AGACTGATAACAAACAGCAGCAAGTACCAGACCAGATGTCGCCCGCGCCAGTTGATTGGCTGAGAAACCAGTGACGGACCGAGCGGCAGCCGGG                                                                                             <  SRR4302535.2753504/94‑1 (MQ=60)
    ACTGATAACAAACAGCAGCAAGTACCAGACCAGATGTCGCCCGCGCCAGTTGATTGGCTGAGAAACCAGTGACGGACCGAGCGGCAGCCGGGTG                                                                                           <  SRR4302535.681721/94‑1 (MQ=60)
    ACTGATAACAAACAGCAGCAAGTACCAGACCAGATGTCGCCCGCGCCAGTTGATTGGCTGAGAAACCAGTGACGGACCGAGCGGCAGCCGGGTG                                                                                           >  SRR4302535.1079270/1‑94 (MQ=60)
    ACTGATAACAAACAGCAGCAAGTACCAGACCAGATGTCGCCCGCGCCAGTTGATTGGCTGAGAAACCAGTGACGGACCGAGCGGCAGCCGGGTG                                                                                           >  SRR4302535.4256734/1‑94 (MQ=60)
     CTGATAACAAACAGCAGCAAGTACCAGACCAGATGTCGCCCGCGCCAGTTGATTGGCTGAGAAACCAGTGACGGACCGAGCGGCAGCCGGGTGT                                                                                          >  SRR4302535.986984/1‑94 (MQ=60)
     CTGATAACAAACAGCAGCAAGTACCAGACCAGATGTCGCCCGCGCCAGTTGATTGGCTGAGAAACCAGTGACGGACCGAGCGGCAGCCGGGTGT                                                                                          >  SRR4302535.1831543/1‑94 (MQ=60)
     CTGATAACAAACAGCAGCAAGTACCAGACCAGATGTCGCCCGCGCCAGTTGATTGGCTGAGAAACCAGTGACGGACCGAGCGGCAGCCGGGTGT                                                                                          >  SRR4302535.1208023/1‑94 (MQ=60)
     CTGATAACAAACAGCAGCAAGTACCAGACCAGATGTCGCCCGCGCCAGTTGATTGGCTGACAAACCAGTGACGGACCGAGCGGCAGCCGGGGGT                                                                                          >  SRR4302535.2827134/1‑94 (MQ=60)
      TGATAACAAACAGCAGCAAGTACCAGACCAGATGTCGCCCGCGCCAGTTGATTGGCTGAGAAACCAGTGACGGACCGAGCGGCAGCCGGGTGTT                                                                                         <  SRR4302535.2200543/94‑1 (MQ=60)
      TGATAACAAACAGCAGCAAGTACCAGACCAGATGTCGCCCGCGCCAGTTGATTGGCTGAGAAACCAGTGACGGACCGAGCGGCAGCCGGGTGTT                                                                                         <  SRR4302535.1775562/94‑1 (MQ=60)
        ATATCAAACAGCAGCAAGTACCAGACCAGATGTCGCCCGCGCCAGTTGATTGGCTGAGAAACCAGTGACGGACCGAGCGGCAGCCGGGTGTTAT                                                                                       >  SRR4302535.3806996/1‑94 (MQ=60)
        ATAACAAACAGCAGCAAGTACCAGACCAGATGTCGCCCGCGCCAGTTGATTGTCTGAGAAACCAGTGACGGACCGAGCGGCAGCCGGGTGTTAT                                                                                       <  SRR4302535.817844/94‑1 (MQ=60)
        ATAACAAACAGCAGCAAGTACCAGACCAGATGTCGCCCGCGCCAGTTGATTGGCTGAGAAACCAGTGACGGACCGAGCGGCAGCCGGGTGTTAT                                                                                       <  SRR4302535.1197067/94‑1 (MQ=60)
        ATAACAAACAGCAGCAAGTACCAGACCAGATGTCGCCCGCGCCAGTTGATTGGCTGAGAAACCAGTGACGGACCGAGCGGCAGCCGGGTGTTAT                                                                                       <  SRR4302535.3823589/94‑1 (MQ=60)
        ATAACAAACAGCAGCAAGTACCAGACCAGATGTCGCCCGCGCCAGTTGATTGGCTGAGAAACCAGTGACGGACCGAGCGGCAGCCGGGTGTTAT                                                                                       <  SRR4302535.1060418/94‑1 (MQ=60)
        ATAACAAACAGCAGCAAGTACCAGACCAGATGTCGCCCGCGCCAGTTGATTGGCTGAGAAACCAGTGACGGACCGAGCGGCAGCCGCGTGTTAT                                                                                       >  SRR4302535.282084/1‑94 (MQ=60)
         TAACAAACAGCAGCAAGTACCAGACCAGATGTCGCCCGCGCCAGTTGATTGGCTGAGAAACCAGTGACGGACTGAGCGGCAGCCGGGCGTTATT                                                                                      >  SRR4302535.4621850/1‑94 (MQ=60)
          AACAAACAGCAGCAAGTACCAGACCAGATGTCGCCCGCGCCAGTTGATTGGCTGAGAAACCAGTGACGGACCGAGCGGCAGCCGGGTGTTATTG                                                                                     <  SRR4302535.1353070/94‑1 (MQ=60)
          AACAAACAGCAGCAAGTACCAGACCAGATGTCGCCCGCGCCAGTTGATTGGCTGAGAAACCAGTGACGGACCGAGCGGCAGCCGGGTGTTATTG                                                                                     <  SRR4302535.3766943/94‑1 (MQ=60)
           ACAAACAGCAGCAAGTACCAGACCAGATGTCGCCCGCGCCAGTTGATTGGCTGAGAAACCAGTGACGGACCGAGCGGCAGCCGGGTGTTATTGG                                                                                    <  SRR4302535.3020563/94‑1 (MQ=60)
            CAAACAGCAGCAAGTACCAGACCAGATGTCGCCCGCGCCAGTTGATTGGCTGAGAAACCAGTGACGGACCGAGCGGCAGCCGGGTGTTATTGGC                                                                                   <  SRR4302535.1715217/94‑1 (MQ=60)
             AAACAGCAGCAAGTACCAGACCAGATGTCGCCCGCGCCAGTTGATTGGCTGAGAAACCAGTGACGGACCGAGCGGCAGCCGGGTGTTATTGGCG                                                                                  >  SRR4302535.1219251/1‑94 (MQ=60)
             AAACAGCAGCAAGTACCAGACCAGATGTCGCCCGCGCCAGTTGATTGGCTGAGAAACCAGTGACGGACCGAGCGGCAGACGGGTGTTATTGGCG                                                                                  >  SRR4302535.10904/1‑94 (MQ=60)
              acgtGCAGCAAGTGCCAGACCAGATGCCGCGCGCGCCAGTTGATTGGCTGAGAAACCAGTGACGGACCGAGCGGCAGCCGGGTGTTATTGGCGA                                                                                 <  SRR4302535.3361803/90‑1 (MQ=60)
                 AGCAGCAAGTACCAGACCAGATGTCGCCCGCGCCAGTTGATTGGCTGAGAAACCAGTGACGGACCGAGCGGCAGCCGGGTGTTATTGGCGAGAT                                                                              >  SRR4302535.1667186/1‑94 (MQ=60)
                   CAGCAAGTACCAGACCAGATGTCGCCCGCGCCAGTTGATTGGCTGAGAAATCAGTGACGGACCGAGCGGCAGCCGGGTGTTATTGGCGAGATAG                                                                            <  SRR4302535.3331240/94‑1 (MQ=60)
                   CAGCAAGTACCAGACCAGATGTCGCCCGCGCCAGTTGATTGGCTGAGAAACCAGTGACGGACCGAGCGGCAGCCGGGTGTTATTGGCGAGATAG                                                                            >  SRR4302535.2140262/1‑94 (MQ=60)
                    AGCAAGTACCAGACCAGATGTCGCCCGCGCCAGTTGATTGGCTGAGAAACCAGTGACGGACCGAGCGGCAGCCGGGTGTTATTGGCGAGATAGT                                                                           <  SRR4302535.2570057/94‑1 (MQ=60)
                    AGCAAGTACCAGACCAGATGTCGCCCGCGCCAGTTGATTGGCTGAGAAACCAGTGACGGACCGAGCGGCAGCCGGGTGTTATTGGCGAGATAGT                                                                           <  SRR4302535.3294295/94‑1 (MQ=60)
                    AGCAAGTACCAGACCAGATGTCGCCCGCGCCAGTTGATTGGCTGAGAAACCAGTGACGGACCGAGCGGCAGCCGGGTGTTATTGGCGAGATAGG                                                                           >  SRR4302535.2161457/1‑94 (MQ=60)
                     GCAAGTACCAGGCCAGATGGCGCCCGCGCCAGTTGATTGGCTGAGAAACCAGTGACGGACCGAGCGGCAGCCGGGTGTTATTGGCGAGATAGTG                                                                          <  SRR4302535.3145276/94‑1 (MQ=60)
                     GCAAGTACCAGACCAGATGTCGCCCGCGCCAGTTGATTGGCTGAGAAACCAGTGACGGACCGAGCGGCAGCCGGGTGTTATTGGCGAGATAGTG                                                                          <  SRR4302535.1294983/94‑1 (MQ=60)
                      CAAGTACCAGACCAGATGTCGCCCGCGCCAGTTGATTGGCTGAGAAACCAGTGACGGACCGAGCGGCAGCCGGGTGTTATTGGCGAGATAGTGC                                                                         >  SRR4302535.2425861/1‑94 (MQ=60)
                      CAAGTACCAGACCAGATGTCGCCCGCGCCAGTTGATTGGCTGAGAAACCAGTGACGGACCGAGCGGCAGCCGGGGGTTATTGGCGAGATAGGGC                                                                         >  SRR4302535.4003670/1‑94 (MQ=60)
                       AAGTACCAGACCAGATGTCGCCCGCGCCAGTTGATTGGCTGAGAAACCAGTGACGGACCGAGCGGCAGCCGGGTGTTATTGGCGAGATAGTGCC                                                                        >  SRR4302535.3565713/1‑94 (MQ=60)
                       AAGTACCAGACCAGATGTCGCCCGCGCCAGTTGATTGGCTGAGAAACCAGTGACGGACCGAGCGGCAGCCGGGTGTTATTGGCGAGATAGTGCC                                                                        >  SRR4302535.4054903/1‑94 (MQ=60)
                        AGTACCAGACCAGATGTCGCCCGCGCCAGTTGATTGGCTGAGAAACCAGTGACGGACCGAGCGGCAGCCGGGTGTTATTGGCGAGATAGTGCCA                                                                       >  SRR4302535.2556965/1‑94 (MQ=60)
                        AGTACCAGACCAGATGTCGCCCGCGCCAGTTGATTGGCTGAGAAACCAGTGACGGACCGAGCGGCAGCCGGGTGTTATTGGCGAGATAGTGCCA                                                                       >  SRR4302535.2556966/1‑94 (MQ=60)
                         GTACCAGACCAGATGTCGCCCGCGCCAGTTGATTGGCTGAGAAACCAGTGACGGACCGAGCGGCAGCCGGGTGTTATTGGCGAGATAGTGCCAG                                                                      >  SRR4302535.3219519/1‑94 (MQ=60)
                            CCAGACCAGATGTCGCCCGCGCCAGTTGATTGGCTGAGAAACCAGTGACGGACCGAGCGGCAGCCGGGTGTTATTGGCGAGATAGTGCCAGAAC                                                                   <  SRR4302535.1177729/94‑1 (MQ=60)
                            CCAGACCAGATGTCGCCCGCGCCAGTTGATTGGCTGAGAAACCAGTGACGGACCGAGCGGCAGCCGGGTGTTATTGGCGAGATAGTGCCAGAAC                                                                   <  SRR4302535.4362128/94‑1 (MQ=60)
                             CAGACCAGATGTCGCCCGCGCCAGTTGATTGGCTGAGAAACCAGTGACGGACCGAGCGGCAGCCGGGTGTTATTGGCGAGATAGTGGCAGAACA                                                                  >  SRR4302535.290361/1‑94 (MQ=60)
                             CAGACCAGATGTCGCCCGCGCCAGTTGATTGGCTGAGAAACCAGTGACGGACCGAGCGGCAGCCGGGTGTTATTGGCGAGATAGTGCCAGAACA                                                                  <  SRR4302535.876687/94‑1 (MQ=60)
                             CAGACCAGATGTCGCCCGCGCCAGTTGATTGGCTGAGAAACCAGTGACGGACCGAGCGGCAGCCGGGTGTTATTGGCGAGATAGTGCCAGAACA                                                                  <  SRR4302535.2897725/94‑1 (MQ=60)
                              AGACCAGATGTCGCCCGCGCCAGTTGATTGGCTGAGAAACCAGTGACGGACCGAGCGGCAGCCGGGTGTTATTGGCGAGATAGTGGCAGAACAC                                                                 >  SRR4302535.855032/1‑94 (MQ=60)
                              AGACCAGATGTCGCCCGCGCCAGTTGATTGGCTGAGAAACCAGTGACGGACCGAGCGGCAGCCGGGTGTTATTGGCGAGATAGTGCCAGAACAC                                                                 <  SRR4302535.179209/94‑1 (MQ=60)
                              AGACCAGATGTCGCCCGCGCCAGTTGATTGGCTGAGAAACCAGTGACGGACCGAGCGGCAGCCGGGTGTTATTGGCGAGATAGTGCCAGAACAC                                                                 >  SRR4302535.1502409/1‑94 (MQ=60)
                              AGACCAGATGTCGCCCGCGCCAGTTGATTGGCTGAGAAACCAGTGACGGACCGAGCGGCAGCCGGGTGTTATTGGCGAGATAGTGCCAGAACAC                                                                 <  SRR4302535.4414279/94‑1 (MQ=60)
                              AGACCAGATGTCGCCCGCGCCAGTTGATTGGCTGAGAAACCAGTGACGGACCGAGCGGCAGCCGGGTGTTATTGGCGAGATAGTGCCAGAACAC                                                                 <  SRR4302535.1867645/94‑1 (MQ=60)
                              AGACCAGATGTCGCCCGCGCCAGTTGATTGGCTGAGAAACCAGTGACGGACCGAGCGGCAGCCGGGTGTTATTGGCGAGATAGTGCCAGAACAC                                                                 >  SRR4302535.539535/1‑94 (MQ=60)
                               GACCAGATGTCGCCCGCGCCAGTTGATTGGCTGAGAAACCAGTGACGGACCGAGCGGCAGCCGGGTGTTATTGGCGAGATAGTGCCAGAACACC                                                                >  SRR4302535.2685401/1‑94 (MQ=60)
                               GACCAGATGTCGCCCGCGCCAGTTGATTGGCTGAGAAACCAGTGACGGACCGAGCGGCAGCCGGGTGTTATTGGCGAGATAGTGCCAGAACACC                                                                >  SRR4302535.1965772/1‑94 (MQ=60)
                                CCCAGATGTCGCCCGCGCCAGTTGATTGGCTGAGAAACCAGTGACGGACCGAGCGGCAGCCGGGTGTTATTGGCGAGATAGTGCCAGAACACCA                                                               <  SRR4302535.1696576/94‑1 (MQ=60)
                                  CAGATGTCGCCCGCGCCAGTTGATTGGCTGAGAAACCAGTGACGGACCGAGCGGCAGCCGGGTGTTATTGGCGAGATAGTGCCAGAACACCAGA                                                             <  SRR4302535.2597460/94‑1 (MQ=60)
                                  CAGATGTCGCCCGCGCCAGTTGATTGGCTGAGAAACCAGTGACGGACCGAGCGGCAGCCGGGTGTTATTGGCGAGATAGTGCCAGAACACCAGA                                                             <  SRR4302535.1111285/94‑1 (MQ=60)
                                  CAGATGTCGCCCGCGCCAGTTGATTGGCTGAGAAACCAGTGACGGACCGAGCGGCAGCCGGGTGTTATTGGCGAGATAGTGCCAGAACACCAGA                                                             <  SRR4302535.3921809/94‑1 (MQ=60)
                                   cgacGTCGCCCGCGCCAGTTGATTGGCTGAGAAACCAGTGACGGACCGAGCGGCAGCCGGGTGTTATTGGCGAGATAGTGCCAGAACACCAGAC                                                            <  SRR4302535.15066/90‑1 (MQ=60)
                                   AGATGTCGCCCGCGCCAGTTGATTGGCTGAGAAACCAGTGACGGACCGAGCGGCAGCCGGGTGTTATTGGCGAGATAGTGCCAGAACACCAGAC                                                            >  SRR4302535.740226/1‑94 (MQ=60)
                                    GATGTCGCCCGCGCCAGTTGATTGGCTGAGAAACCAGTGACGGACCGAGCGGCAGCCGGGTGTTATTGGCGAGATAGTGCCAGAACACCAGACA                                                           <  SRR4302535.4381410/94‑1 (MQ=60)
                                     ATGTCGCCCGCGCCAGTTGATTGGCTGAGAAACCAGTGACGGACCGAGCGGCAGCCGGGTGTTATTGGCGAGATAGTGCCAGAACACCAGACAT                                                          <  SRR4302535.606706/94‑1 (MQ=60)
                                       GTCGCCCGCGCCAGTTGATTGGCTGAGAAACCAGTGACGGACCGAGCGGCAGCCGGGTGTTATTGGCGAGATAGTGCCAGAACACCAGACATAT                                                        <  SRR4302535.3909174/94‑1 (MQ=60)
                                       GTCGCCCGCGCCAGTTGATTGGCTGAGAAACCAGTGACGGACCGAGCGGCAGCCGGGTGTTATTGGCGAGATAGTGCCAGAACACCAGACATAT                                                        <  SRR4302535.3749669/94‑1 (MQ=60)
                                        TCGCCCGCGCCAGTTGATTGGCTGAGAAACCAGTGACGGACCGAGCGGCAGCCGGGTGTTATTGGCGAGATAGTGCCAGAACACCAGACATATC                                                       <  SRR4302535.824065/94‑1 (MQ=60)
                                         CGNCNCNGCCAGTTAATTGCCNNAGAAACCAGTGACGGACCGAGCGGCAGCCGGGTGTTATTGGCGAGATAGTGCCAGAACACCAGACATATCG                                                      <  SRR4302535.1899441/94‑1 (MQ=60)
                                         CGCCCGCGCCAGTTGATTGTCTGAGAAACCAGTGACGGACCGAGCGGCAGCCGGGTGTTATTGGCGAGATAGTGCCAGAACACCAGACATATCG                                                      <  SRR4302535.2083825/94‑1 (MQ=60)
                                         CGCCCGCGCCAGTTGATTGGCTGAGAAACCAGTGACGGACCGAGCGGCAGCCGGGTGTTATTGGCGAGATAGTGCCAGAACACCAGACATATCG                                                      <  SRR4302535.871541/94‑1 (MQ=60)
                                         CGCCCGCGCCAGTTGATTGGCTGAGAAACCAGTGACGGACCGAGCGGCAGCCGGGTGTTATTGGCGAGATAGTGCCAGAACACCAGACATATCG                                                      <  SRR4302535.1642078/94‑1 (MQ=60)
                                          GCCCGCGCCAGTTGATTGGCTGAGAAACCAGTGACGGACCGAGCGGCAGCCGGGTGTTATTGGCGAGATAGTGCCAGAACACCAGACATATCGG                                                     <  SRR4302535.3107263/94‑1 (MQ=60)
                                           CCCGCGCCAGTTGATTGGCTGAGAAACCAGTGACGGACCGAGCGGCAGCCGGGTGTTATTGGCGAGATAGTGCCAGAACACCAGACATATCGGG                                                    >  SRR4302535.2703626/1‑94 (MQ=60)
                                           CCCGCGCCAGTTGATTGGCTGAGAAACCAGTGACGGACCGAGCGGCAGCCGGGTGTTATTGGCGAGATAGTGCCAGAACACCAGACATATCGGG                                                    >  SRR4302535.4633710/1‑94 (MQ=60)
                                            CCGCGCCAGTTGATTGGCTGAGAAACCAGTGACGGACCGAGCGGCAGCCGGGTGTTATTGGCGAGATAGTGCCAGAACACCAGACATATCGGGG                                                   <  SRR4302535.3613651/94‑1 (MQ=60)
                                             CGCGCCAGTTGATTGGCTGAGAAACCAGTGACGGACCGAGCGGCAGCCGGGTGTTATTGGCGAGATAGTGCCAGAACACCAGACATATCGGGGC                                                  <  SRR4302535.2212033/94‑1 (MQ=60)
                                              GCGCCAGTTGATTGGCTGAGAAACCAGTGACGGACCGAGCGGCAGCCGGGTGTTATTGGCGAGATAGTGCCAGAACACCAGACATATCGGGGCC                                                 <  SRR4302535.1847278/94‑1 (MQ=60)
                                              GCGCCAGTTGATTGGCTGAGAAACCAGTGACGGACCGAGCGGCAGCCGGGTGTTATTGGCGAGATAGTGCCAGAACACCAGACATATCGGGGCC                                                 <  SRR4302535.1909973/94‑1 (MQ=60)
                                              GCGCCAGTTGATTGGCTGAGAAACCAGTGACGGACCGAGCGGCAGCCGGGTGTTATTGGCGAGATAGTGCCAGAACACCAGACATATCGGGGCC                                                 >  SRR4302535.4230033/1‑94 (MQ=60)
                                               CGCCAGTTGATTGGCTGAGAAACCAGTGACGGACCGAGCGGCAACCGGGTGTTATCGGCGAGATAGTGCCAGAACACCAGACATATCGGGGCCA                                                <  SRR4302535.2704780/94‑1 (MQ=60)
                                                GCCAGTTGATTGGCTGAGAAACCAGTGACGGACCGAGCGGCAGCCGGGTGTTATTGGCGAGATAGTGCCAGAACACCAGACATATCGGGGCCAG                                               >  SRR4302535.2906963/1‑94 (MQ=60)
                                                GCCAGTTGATTGGCTGAGAAACCAGTGACGGACCGAGCGGCAGCCGGGTGTTATTGGCGAGATAGTGCCAGAACACCAGACATATCGGGGCCAG                                               >  SRR4302535.4400561/1‑94 (MQ=60)
                                                GCCAGTTGATTGGCTGAGAAACCAGTGACGGACCGAGCGGCAGCCGGGGGTTATTGGCGAGATAGTGCCAGAACACCAGACATATCGGGGCCAG                                               >  SRR4302535.3698101/1‑94 (MQ=60)
                                                  CAGTTGATTGGCTGAGAAACCAGTGACGGACCGAGCGGCAGCCGGGTGTTATTGGCGAGATAGTGCCAGAACACCAGACATATCGGGGCCAGCG                                             <  SRR4302535.4197594/94‑1 (MQ=60)
                                                    GTTGATTGGCTGAGAAACCAGTGACGGACCGAGCGGCAGCCGGGTGTTATTGGCGAGATAGTGCCAGAACACCAGACATATCGGGGTCAGCGTC                                           >  SRR4302535.2057342/1‑94 (MQ=60)
                                                     TTGATTGGCTGAGAAACCAGTGACGGACCGAGCGGCAGCCGGGTGTTATTGGCGAGATAGTGCCAGAACACCAGACATATCGGGGCCAGCGTCA                                          >  SRR4302535.529263/1‑94 (MQ=60)
                                                      TGATTGGCTGAGAAACCAGTGACGGACCGAGCGGCAGCCGGGTGTTATTGGCGAGATAGTGCCAGAACACCAGACATATCGGGGCCAGCGTCAG                                         >  SRR4302535.2473044/1‑94 (MQ=60)
                                                      TGATTGGCTGAGAAACCAGTGACGGACCGAGCGGCAGCCGGGTGTTATTGGCGAGATAGGACCCAAACCCCAGACATATCGGGGCCAGCGTCAG                                         >  SRR4302535.3547188/1‑94 (MQ=60)
                                                       GATTGGCTGAGAAACCAGTGACGGACCGAGCGGCAGCCGGGTGTTATTGGCGAGATAGTGCCAGAACACCAGACATATCGGGGCCAGCGTCAGG                                        >  SRR4302535.4515184/1‑94 (MQ=60)
                                                       GATTGGCTGAGAAACCAGTGACGGACCGAGCGGCAGCCGGGTGTTATTGGCGAGATAGTGCCAGAACACCAGACATATCGGGGCCAGCGTCAGG                                        >  SRR4302535.2903654/1‑94 (MQ=60)
                                                        ATTGGCTGAGAAACCAGTGACGGACCGAGCGGCAGCCGGGTGTTATTGGCGAGATAGTGCCAGAACACCAGACATATCGGGGCCAGCGTCAGGC                                       >  SRR4302535.3872606/1‑94 (MQ=60)
                                                        ATTGGCTGAGAAACCAGTGACGGACCGAGCGGCAGCCGGGTGTTATTGGCGAGATAGTGCCAGAACACCAGACATATCGGGGCCAGCGTCAGGC                                       >  SRR4302535.3837826/1‑94 (MQ=60)
                                                         TTGGCTGAGAAACCAGTGACGGACCGAGCGGCAGCCGGGGGTTATTGGCGAGATAGTGCCAGAACACCAGACATATCGGGGCCAGCGTCATGCC                                      >  SRR4302535.4645269/1‑94 (MQ=60)
                                                           GGCTGAGAAACCAGTGACGGACCGAGCGGCAGCCGGGTGTTATTGGCGAGATAGTGCCAGAACACCAGACATATCGGGGCCAGCGTCAGGCCGC                                    >  SRR4302535.2031876/1‑94 (MQ=60)
                                                              TGAGAAACCAGTGACGGACCGAGCGGCAGCCGGGTGTTATTGGCGAGATAGTGCCAGAACACCAGACATATCGGGGCCAGCGTCAGGCCGCCAG                                 >  SRR4302535.3798/1‑94 (MQ=60)
                                                              TGAGAAACCAGTGACGGACCGAGCGGCAGCCGGGTGTTATTGGCGAGATAGTGCCAGAACACCAGACATATCGGGGCCAGCGTCAGGCCGCCAG                                 >  SRR4302535.1068694/1‑94 (MQ=60)
                                                               GAGAAACCAGTGACGGACCGAGCGGCAGCCGGGTGTTATTGGCGAGATAGTGCCAGAACACCAGACATATCGGGGCCAGCGTCAGGCCGCCAGT                                >  SRR4302535.2982321/1‑94 (MQ=60)
                                                                AGAAACCAGTGACGGACCGAGCGGCAGCCGGGTGTTATTGGCGTGATAGTGCCAGAACACCAGACATATCGGGGCCAGCGTCAGGCCGCCAGTT                               >  SRR4302535.2529551/1‑94 (MQ=60)
                                                                  AAACCAGTGACGGACCGAGCGGCAGCCGGGTGTTATTGGCGAGATAGTGCCAGAACACCAGACATATCGGGGCCAGCGTCAGGCCGCCAGTTAA                             <  SRR4302535.539390/94‑1 (MQ=60)
                                                                  AAACCAGTGACGGACCGAGCGGCAGCCGGGTGTTATTGGCGAGATAGTGCCAGAACACCAGACATATCGGGGCCAGCGTCAGGCCGCCAGTTAA                             <  SRR4302535.2716386/94‑1 (MQ=60)
                                                                  AAACCAGTGACGGACCGAGCGGCAGCCGGGTGTTATTGGCGAGATAGTGCCAGAACACCAGACATATCGGGGCCAGCGTCAGGCCGCCAGTTAA                             >  SRR4302535.2165456/1‑94 (MQ=60)
                                                                   AACCAGTGACGGACCGAGCGGCAGCCGGGTGTTATTGGCGAGATAGTGCCAGAACACCAGACATATCGGGGCCAGCGTCAGGCCGCCAGTTAAA                            >  SRR4302535.70421/1‑94 (MQ=60)
                                                                    ACCAGTGACGGACCGAGCGGCAGCCGGGTGTTATTGGCGAGATAGTGCCAGAACACCAGACATATCGGGGCCAGCGTCAGGCCGCCAGTTAAAG                           <  SRR4302535.810154/94‑1 (MQ=60)
                                                                     CCAGTGACGGCCGGAGCGGC‑GCCGGGTGTTATTGGCGAGATAGTGCCAGAACACCAGACATATCGGGGCCAGCGTCAGGCCGCCAGTTAAAGTC                         <  SRR4302535.3485112/94‑1 (MQ=60)
                                                                      CAGTGACGGACCGAGCGGCAGCCGGGTGTTATTGGCGAGATAGTGCCAGAACACCAGACATATCGGGGCCAGCGTCAGGCCGCCAGTTAAAGTC                         <  SRR4302535.1303508/94‑1 (MQ=60)
                                                                      CAGTGACGGACCGAGCGGCAGCCGGGTGTTATTGGCGAGATAGTGCCAGAACACCAGACATATCGGGGCCAGCGTCAGGCCGCCAGTTAAAGTC                         <  SRR4302535.3071210/94‑1 (MQ=60)
                                                                       AGTGACGGACCGAGCGGCAGCCGGGTGTTATTGGCGAGATAGTGCCAGAACACCAGACATATCGGGGCCAGCGTCAGGCCGCCAGTTAAAGTCA                        <  SRR4302535.1036896/94‑1 (MQ=60)
                                                                       AGTGACGGACCGAGCGGCAGCCGGGTGTTATTGGCGAGATAGTGCCAGAACACCAGACATATCGGGGCCAGCGTCAGGCCGCCAGTTAAAGTCA                        <  SRR4302535.3502024/94‑1 (MQ=60)
                                                                       AGTGACGGACCGAGCGGCAGCCGGGTGTTATTGGCGAGATAGTGCCAGAACACCAGACATATCGGGGCCAGCGTCAGGCCGCCAGTTAAAGTCA                        >  SRR4302535.4134630/1‑94 (MQ=60)
                                                                       AGTGACGGACCGAGCGGCAGCCGGGTGTTATTGGCGAGATAGTGCCAGAACACCAGACATATCGGGGCCAGCGGCAGGCCGCCAGTTAAAGTCA                        >  SRR4302535.745865/1‑94 (MQ=60)
                                                                        GTGACGGACCGAGCGGCAGCCGGGTGTTATTGGCGAGATAGTGCCAGAACACCAGACATATCGGGGCCAGCGTCAGGCCGCCAGTTAAAGTCAG                       <  SRR4302535.1006318/94‑1 (MQ=60)
                                                                        GTGACGGACCGAGCGGCAGCCGGGTGTTATTGGCGAGATAGTGCCAGAACACCAGACATATCGGGGCCAGCGTCAGGCCGCCAGTTAAAGTCAG                       <  SRR4302535.3957914/94‑1 (MQ=60)
                                                                            CGGACCGAGCGGCAGCCGGGTGTTATTGGCGAGATAGTGCCAGAACACCAGACATATCGGGGCCAGCGTCAGGCCGCCAGTTAAAGTCAGCAAC                   <  SRR4302535.2387378/94‑1 (MQ=60)
                                                                              GACCGAGCGGCAGCCGGGTGTTATTGGCGAGATAGTGCCAGAACACCAGACATATCGGGGCCAGCGTCAGGCCGCCAGTTAAAGTCAGCAACAG                 <  SRR4302535.3918828/94‑1 (MQ=60)
                                                                                 CGAGCGGCAGCCGGGTGTTATTGGCGAGATAGTGCCAGAACACCAGACATATCGGGGCCAGCGTCAGGCCGCCAGTTAAAGTCAGCAACAGCGC              >  SRR4302535.3011571/1‑94 (MQ=60)
                                                                                 CGAGCGGCAGCCGGGTGTTATTGGCGAGATAGTGCCAGAACACCAGACATATCGGGGCCAGCGTCAGGCCGCCAGTTAAAGTCAGCAACAGCGC              >  SRR4302535.2340994/1‑94 (MQ=60)
                                                                                   AGCGGCAGCCGGGTGTTATTGGCGAGATAGTGCCAGAACACCAGACATATCGGGGCCAGCGTCAGGCCGCCAGTTAAAGTCAGCAACAGCGCAT            <  SRR4302535.802247/94‑1 (MQ=60)
                                                                                   AGCGGCAGCCGGGTGTTATTGGCGAGATAGTGCCAGAACACCAGACATATCGGGGCCAGCGTCAGGCCGCCAGTTAAAGTCAGCAACAGCGCAT            >  SRR4302535.451782/1‑94 (MQ=60)
                                                                                      GGCAGCCGGGTGTTATTGGCGAGATAGTGCCAGAACACCAGACATATCGGGGCCAGCGTCAGGCCGCCAGTTAAAGTCAGCAACAGCGCATTCC         <  SRR4302535.3253676/94‑1 (MQ=60)
                                                                                       GCAGCCGGGTGTTATTGGCGAGATAGTGCCAGAACACCAGACATATCGGGGCCAGCGTCAGGCCGCCAGTTAAAGTCAGCAACAGCGCATTCCA        >  SRR4302535.811228/1‑94 (MQ=60)
                                                                                        CAGCCGGGTGTTATTGGCGAGATAGTGCCAGAACACCAGACATATCGGGGCCAGCGTCAGGCCGCCAGTTAAAGTCAGCAACAGCGCATTCCAC       <  SRR4302535.37283/94‑1 (MQ=60)
                                                                                        CAGCCGGGTGTTATTGGCGAGATAGTGCCAGAACACCAGACATATCGGGGCCAGCGTCAGGCCGCCAGTTAAAGTCAGCAACAGCGCATTCCAC       <  SRR4302535.3128966/94‑1 (MQ=60)
                                                                                        CAGCCGGGTGTTATTGGCGAGATAGTGCCAGAACACCAGACATATCGGGGCCAGCGTCAGGCCGCCAGTTAAAGTCAGCAACAGCGCATTCCAC       <  SRR4302535.845502/94‑1 (MQ=60)
                                                                                         AGCCGGGTGTTATTGGCGAGATAGTGCCAGAACACCAGACATATCGGGGCCAGCGTCAGGCCGCCAGTTAAAGTCAGCAACAGCGCATTCCACG      <  SRR4302535.4091550/94‑1 (MQ=60)
                                                                                            CGGGTGTTATTGGCGAGATAGTGCCAGAACACCAGACATATCGGGGCCAGCGTCAGGCCGCCAGTTAAAGTCAGCAACAGCGCATTCCACGACT   <  SRR4302535.2145459/94‑1 (MQ=60)
                                                                                            CGGGTGTTATTGGCGAGATAGTGCCAGAACACCAGACATATCGGGGCCAGCGTCAGGCCGCCAGTTAAAGTCAGCAACAGCGCATTCCACGACT   <  SRR4302535.4312802/94‑1 (MQ=60)
                                                                                             GGGTGTTATTGGCGAGATAGTGCCAGAACACCAGACATATCGGGGCCAGCGTCAGGCCGCCAGTTAAAGTCAGCAACAGCGCATTCCACGACTC  <  SRR4302535.3123892/94‑1 (MQ=60)
                                                                                             |                                                                                             
AGAGACTGATAACAAACAGCAGCAAGTACCAGACCAGATGTCGCCCGCGCCAGTTGATTGGCTGAGAAACCAGTGACGGACCGAGCGGCAGCCAGGTGTTATTGGCGAGATAGTGCCAGAACACCAGACATATCGGGGCCAGCGTCAGGCCGCCAGTTAAAGTCAGCAACAGCGCATTCCACGACTC  >  NC_000913/3847172‑3847358

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑