Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I2 R1	1873	183.1	9376148	98.2%	9207377	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,957,560	+C	intergenic (‑129/‑732)	ppiC ← / → rep	peptidyl‑prolyl cis‑trans isomerase C/rep helicase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,957,558	1	.	C	98.9%	582.7 / ‑5.1	184	intergenic (‑127/‑734)	ppiC/rep	peptidyl‑prolyl cis‑trans isomerase C/rep helicase
Reads supporting (aligned to +/- strand): ref base . (2/0); major base C (84/96); minor base A (1/1); total (87/97)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 196 total aligned reads displayed.

TTAGCATGATCCGCAGATGCTAACGTAATTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCC‑GGAAAAT‑CC‑‑T‑GC‑TCGCAGG‑T‑CC‑TCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTGTCAATTTATGAGCGATATG  >  NC_000913/3957469‑3957645

   gCATGATCCGCAGATGCTAACGTAATTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCC‑GGAAAAT‑CCG‑G‑GCTTCGCAGG‑T‑ccct                                                                                      >  2:4653394/1‑91 (MQ=255)

     aTGATCCGCAGATGCTAACGTAATTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCC‑GGAAAAT‑CCG‑G‑GCTTCGCAGG‑T‑ccctca                                                                                    >  2:3178309/1‑89 (MQ=255)

     aTGATCCGCAGATGCTAACGTAATTGTCAGTCAGGGAGTGGCTGGGACATATATGGCAAAAGCCC‑GGGAAAT‑CCG‑G‑GCTTCGCAGG‑T‑ccctcc                                                                                    >  2:2400370/1‑89 (MQ=255)

        aTCCGCAGATGCTAACGTAATTGTCAGTCGGGGAGTGGCTGGTACATATATGGCAAAAGCCC‑GGAAAAT‑CCG‑G‑GCTTCGCAGG‑TCCC‑TCAAgg                                                                                 <  1:1846983/93‑1 (MQ=255)

        aTCCGCAGATGCTAACGTAATTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCC‑GGAAAAT‑CCG‑G‑GCTTCGCAGG‑TCCC‑TCAAgg                                                                                 <  2:4503788/93‑1 (MQ=255)

        aTCCGCAGATGCTAACGTAATTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCC‑GGAAAAT‑CCG‑G‑GCTTCGCAGG‑TCCC‑TCAAgg                                                                                 <  1:4443476/93‑1 (MQ=255)

         tCCGCAGATGCTAACGTAATTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCC‑GGAAAAT‑CCG‑G‑GCTTCGCAGG‑TCCC‑TCAAggg                                                                                <  1:3730967/93‑1 (MQ=255)

         tCCGCAGATGCTAACGTAATTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCC‑GGAAAAT‑CCG‑G‑GCTTCGCAGG‑TCCC‑TCAAggg                                                                                >  2:4453096/1‑93 (MQ=255)

          ccGCAGATGCTAACGTAATTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCC‑GGAAAAT‑CCG‑G‑GCTTCGCAGG‑TCCC‑TCAAgggg                                                                               <  2:1534196/93‑1 (MQ=255)

            gCAGATGCTAACGTAATTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCC‑GGAAAAT‑CCG‑G‑GCTTCGCAGG‑TCCC‑TCAAgggggg                                                                             >  1:1666371/1‑91 (MQ=255)

            gCAGATGCTAACGTAATTGTCAGTCAGGGAGTGGCTGGGACATATATGGCAAAAGCCC‑GGAAAAT‑CCG‑G‑GCTTCGCAGG‑TCCC‑TCAAGGGgag                                                                             >  1:2855760/1‑93 (MQ=255)

             cAGATGCTAACGTAATTGTCAGTCAGGGAGTGGCTGGTTCCTATATCGCAAAAGCCCGGGAAAAA‑CG‑‑G‑GCGTCGCAGG‑TCCC‑TCAAAGGgaga                                                                            >  1:4587355/1‑93 (MQ=255)

              aGATGCTAACGTAATTGTCAGTCAGGGAGTGGCTGGTACATATATGGGAAAAGCCC‑GGAAAATCCC‑‑G‑GCTTCGCAAG‑TACC‑TCAAGGGgagca                                                                           >  1:4140935/1‑91 (MQ=255)

              aGATGCTAACGTAATTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCC‑GGAAAAT‑CCG‑G‑GCTTCGCAGG‑TCCC‑TCAAGGGgagga                                                                           >  1:3216590/1‑91 (MQ=255)

               gATGCTAACGTAATTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCC‑GGAAAAT‑CCG‑G‑GCTTCGCAGG‑TCCC‑TCAAGGGGAGaaa                                                                          >  2:367432/1‑93 (MQ=255)

               gATGCTAACGTAATTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCC‑GGAAAAT‑CCG‑G‑GCTTCGCAGG‑TCCC‑TCAAGGGGAGaaa                                                                          >  2:2703296/1‑93 (MQ=255)

                 tGCTAACGTAATTGTCAGTCAGGGAGTGGCTGGGACATATATGGCAAAAGCCC‑GGAAAAT‑CCG‑G‑GCTTCGCAGG‑TCCC‑TCAAGGGGAGAAAAc                                                                        >  2:3318892/1‑93 (MQ=255)

                   cTAACGTAATTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCC‑GGAAAAT‑CCG‑G‑GCTTCGCAGGAC‑CC‑TCAAGGGGAGAAAACtt                                                                      >  1:1284556/1‑93 (MQ=255)

                   cTAACGTAATTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCC‑GGAAAAT‑CCG‑G‑GCTTCGCAGG‑TCCC‑TCAAGGGGAGAAAACtt                                                                      >  1:4197072/1‑93 (MQ=255)

                    tAACGTAATTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCC‑GGAAAAT‑CC‑‑GGGCTTCGCAGG‑TCCC‑TCAAGGGGAGAAAACTTa                                                                     <  2:2275360/93‑1 (MQ=255)

                     aaCGTAATTGTCAGTCAGGGAGTGGCTGTTACATATATGGCAAAATCCC‑GGAAAAT‑CCG‑G‑GCTTCGCAGG‑TCCC‑TCAAGGGGAGAAAACTTAg                                                                    <  2:4067072/93‑1 (MQ=255)

                     aaCGTAATTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCC‑GGAAAAT‑CCG‑G‑GCTTCGCAGG‑TCCC‑TCAAGGGGAGAAAACTTAg                                                                    <  2:1949860/93‑1 (MQ=255)

                       cGTAATTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCC‑GGAAAAT‑CCG‑G‑GCTTCGCAGG‑TCCC‑TCAAGGGGAGAAAACTTAggg                                                                  <  1:784265/93‑1 (MQ=255)

                        gTAATTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCC‑GGAAAAT‑CCG‑G‑GCTTCGCAGG‑TCCC‑TCAAGGGGAGAAAACTTAGGGc                                                                 <  2:3910044/93‑1 (MQ=255)

                         tAATTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCC‑GGAAAAT‑CCGGG‑GT‑TCGCAGG‑TCCC‑CCAAGGGGAGAAAACTTAggggc                                                                >  2:2508064/1‑91 (MQ=255)

                         tAATTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCC‑GGAAAAT‑CCG‑G‑GCTTCGCAGG‑TCCC‑TCAAGGGGAGAAAACTTAGGGcc                                                                >  1:3918564/1‑93 (MQ=255)

                         tAATTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCC‑GGAAAAT‑CCG‑G‑GCTTCGCAGG‑TCCC‑TCAAGGGGAGAAAACTTAGGGcc                                                                <  1:2078765/93‑1 (MQ=255)

                          aaTTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCC‑GGAAAAT‑CCG‑G‑GCTTCGCAGG‑TCCC‑TCAAGGGGAGAAAACTTAGGGCct                                                               >  1:3329894/1‑93 (MQ=255)

                           atggtcagtcaGGGAGTGGCTGGTACATATATGGCAAAAGCCC‑GGAAAAT‑CC‑‑GGGCTTCGCAGG‑TCCC‑TCAAGGGGAGAAAACTTAGGGCctc                                                              <  2:677083/90‑1 (MQ=255)

                           aTTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCC‑GGAAAAT‑CCG‑G‑GCTTCGCAGG‑TCCC‑TCAAGGGGAGAAAACTTAGGGCctc                                                              <  1:1020261/93‑1 (MQ=255)

                             tGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCC‑GGAAAAT‑CCG‑G‑GCTTCGCAGG‑TCCC‑TCAAGGGGAGAAAACTTAGGGCCTCTa                                                            <  2:1273965/93‑1 (MQ=255)

                             tGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCC‑GGAAAAT‑CCG‑G‑GCTTCGCAGG‑TCCC‑TCAAGGGGAGAAAACTTAGGGCCTCTa                                                            <  2:4150698/93‑1 (MQ=255)

                              gtcagtcaGGGAGTGGCTGGTACATATATGGCAAAAGCCC‑GGAAAAT‑CCG‑G‑GCTTCGCAGG‑TCCC‑TCAAGGGGAGAAAACTTAGGGCCTCTAt                                                           <  2:130383/93‑1 (MQ=255)

                              gtcagtcaGGGAGTGGCTGGTACATATATGGCAAAAGCCC‑GGAAAAT‑CCG‑G‑GCTTCGCAGG‑TCCC‑TCAAGGGGAGAAAACTTAGGGCCTCTAt                                                           <  2:3303971/93‑1 (MQ=255)

                               tcagtcagGGAGTGGCTGGTACATATATGGCAAAAGGCC‑GGAAAAT‑CCG‑G‑GCTTCGCAGG‑TCCC‑TCAAGGGGAGAAAACTTAGGGCCTCCATg                                                          >  2:1630245/1‑93 (MQ=255)

                               tcagtcagGGAGTGGCTGGTACATATATGGCAAAAGCCC‑GGAAAAT‑CCG‑G‑GCTTCGCAGG‑TCCC‑TCAAGGGGAGAAAACTTAGGGCCTCTATg                                                          <  1:538367/93‑1 (MQ=255)

                                  gtcagGGAGTGGCTGGTACGTATATGGCAAAAGCCC‑GGAAAAT‑CCG‑G‑GCTTCGCAGG‑TCCC‑TCAAGGGGAGAAAACTTAGGGCCTCTATGGGc                                                       <  2:480700/93‑1 (MQ=255)

                                  gtcagGGAGTGGCTGGTACATATATGGCAAAAGCCC‑GGAAAAT‑CCG‑G‑GCTTCGCAGG‑TCCC‑TCAAGGGGAGAAAACTTAGGGCCTCTATGGGc                                                       <  2:2999988/93‑1 (MQ=255)

                                    cagGGAGTGGCTGGTACATATATGGCAAAAGCCC‑GGAAAAT‑CCG‑G‑GCTTCGCAGG‑TCCC‑TCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTa                                                     <  1:2390802/93‑1 (MQ=255)

                                     agGGAGTGGCTGGTACATATATGGCAAAAGCCC‑GGAAAAT‑CCG‑G‑GCTTCGCAGG‑TCCC‑TCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTAc                                                    <  1:3110353/93‑1 (MQ=255)

                                     agGGAGTGGCTGGTACATATATGGCAAAAGCCC‑GGAAAAT‑CCG‑G‑GCTTCGCAGG‑TCCC‑TCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTAc                                                    >  1:3007812/1‑93 (MQ=255)

                                      gggAGTGGCTGGTACATATATGGCAAAAGCCC‑GGAAAAT‑CCG‑G‑GCTTCGCAGG‑TCCC‑TCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACa                                                   >  2:1766907/1‑93 (MQ=255)

                                      gggAGTGGCTGGTACATATATGGCAAAAGCCC‑GGAAAAT‑CCG‑G‑GCTTCGCAGG‑TCCC‑TCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACa                                                   >  2:241424/1‑93 (MQ=255)

                                      gggAGTGGCTGGTACATATATGGCAAAAGCCC‑GGAAAAT‑CC‑‑GGGCTTCGCAGG‑TCCC‑TCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACa                                                   >  1:1499344/1‑93 (MQ=255)

                                        gAGTGGCTGGTACATATATGGCAAAAGCCC‑GGAAAAT‑CCG‑G‑GCTTCGCAGG‑TCCC‑TCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACaaa                                                 <  1:1416845/93‑1 (MQ=255)

                                          gTGGCTGGTACATATATGGCAAAAGCCC‑GGAAAAT‑CCG‑G‑GCTTCGCAGG‑TCCC‑TCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGt                                               <  2:4116896/93‑1 (MQ=255)

                                          gTGGCTGGTACATATATGGCAAAAGCCC‑GGAAAAT‑CCG‑G‑GCTTCGCAGG‑TCCC‑TCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGt                                               >  2:956580/1‑93 (MQ=255)

                                           tGGCTGGTACATATATGGCAAAAGCCC‑GGAAAAT‑CC‑‑GGGCTTCGCAGG‑TCCC‑TCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTc                                              >  1:98210/1‑93 (MQ=255)

                                            ggCTGGTACATATATGGCAAAAGCCC‑GGAAAAT‑CCG‑G‑GCTTCGCAGG‑TCCC‑TCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCa                                             <  2:3399515/93‑1 (MQ=255)

                                            ggCTGGTACATATATGGCAAAAGCCC‑GGAAAAT‑CCG‑G‑GCTTCGCAGG‑TCCC‑TCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCa                                             >  2:4242645/1‑93 (MQ=255)

                                            ggCTGGTACATATATGGCAAAAGCCC‑GGAAAAT‑CCG‑G‑GCTTCGCAGG‑TCCC‑TCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCa                                             <  1:1675130/93‑1 (MQ=255)

                                            ggCTGGTACATATATGGCAAAAGCCC‑GGAAAAT‑CCG‑G‑GCTTCGCAGG‑TCCC‑TCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCa                                             <  1:2609307/93‑1 (MQ=255)

                                            ggCTGGTACATATATGGCAAAAGCCC‑GGAAAAT‑CCG‑G‑GCTTCGCAGG‑TCCC‑TCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCa                                             <  1:395835/93‑1 (MQ=255)

                                               tGGTACATATATGGCAAAAGCCC‑GGAAAAT‑CCG‑G‑GCTTCGCAGG‑TCCC‑TCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTa                                          <  2:1197357/93‑1 (MQ=255)

                                                ggTACATATATGGCAAAAGCCC‑GGAAAAT‑CCG‑G‑GCTTCGCAGG‑TCCC‑TCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTAt                                         <  2:4287924/93‑1 (MQ=255)

                                                 gTACATATATGGCAAAAGCCC‑GGAAAAT‑CCG‑G‑GCTTCGCAGG‑TCCC‑TCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATc                                        >  1:3559288/1‑93 (MQ=255)

                                                 gTACATATATGGCAAAAGCCC‑GGAAAAT‑CCG‑G‑GCTTCGCAGG‑TCCC‑TCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATc                                        >  1:3286258/1‑93 (MQ=255)

                                                   aCATATATGGCAAAAGCCC‑GGAAAAT‑CCG‑G‑GCTTCGCAGG‑TCCC‑TCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCgg                                      <  1:1031198/93‑1 (MQ=255)

                                                    cATATATGGCAAAAGCCC‑GGAAAAT‑CCG‑G‑GCTTCGCAGG‑TCCC‑TCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCggg                                     >  1:4676919/1‑93 (MQ=255)

                                                    cATATATGGCAAAAGCCC‑GGAAAAT‑CC‑‑GGGCTTCGCAGG‑TCCC‑TCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCggg                                     >  2:3766126/1‑93 (MQ=255)

                                                     atatatGGCAAAAGCCC‑GGAAAAT‑CCG‑G‑GCTTCGCAGG‑TCCC‑TCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCgggg                                    >  1:4603493/1‑93 (MQ=255)

                                                      tatatGGCAAAAGCCC‑GGAAAAT‑CCG‑G‑GCTTCGCAGG‑TCCC‑TCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGa                                   >  1:1325728/1‑93 (MQ=255)

                                                       atatGGCAAAAGCCC‑GGAAAAT‑CCG‑G‑GCTTCGCAGG‑TCCC‑TCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGAt                                  <  2:265810/93‑1 (MQ=255)

                                                        tatGGCAAAAGCCC‑GGAAAAT‑CCG‑G‑GCTTCGCAGG‑TCCC‑TCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATg                                 >  2:510672/1‑93 (MQ=255)

                                                          tGGCAAAAGCCC‑GGAAAAT‑CCG‑G‑GCTTCGCAGG‑TCCC‑TCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGt                               <  2:1462522/93‑1 (MQ=255)

                                                          tGGCAAAAGCCC‑GGAAAAT‑CCG‑G‑GCTTCGCAGG‑TCCC‑TCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGt                               >  1:3142560/1‑93 (MQ=255)

                                                             cAAAAGCCC‑GGAAAAT‑CCG‑G‑GCTTCGCAGG‑TCCC‑TCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGttat                            >  1:4016532/1‑93 (MQ=255)

                                                              aaaaGCCC‑GGAAAAT‑CCG‑G‑GCTTCGCAGG‑TCCC‑TCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGCTGGttatt                           >  1:2239000/1‑93 (MQ=255)

                                                              aaaaGCCC‑GGAAAAT‑CCG‑G‑GCTTCGCAGG‑TCCC‑TCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGttatt                           >  1:2945734/1‑93 (MQ=255)

                                                              aaaaGCCC‑GGAAAAT‑CCG‑G‑GCTTCGCAGG‑TCCC‑TCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGttatt                           <  1:1949039/93‑1 (MQ=255)

                                                              aaaaGCCC‑GGAAAAT‑CCG‑G‑GCTTCGCAGG‑TCCC‑TCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGttatt                           >  1:3342342/1‑93 (MQ=255)

                                                              aaaaGCCC‑GGAAAAT‑CCG‑G‑GCTTCGCAGG‑TCCC‑TCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGttatt                           >  2:3647666/1‑93 (MQ=255)

                                                              aaaaGCCC‑GGAAAAT‑CCG‑G‑GCTTCGCAGG‑TCCC‑TCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGttatt                           <  2:3476585/93‑1 (MQ=255)

                                                                 aGCCC‑GGAAAAT‑CCG‑G‑GCTTCGCAGG‑TCCC‑TCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGttatttta                        <  2:1014414/93‑1 (MQ=255)

                                                                   ccc‑GGAAAAT‑CCG‑G‑GCTTCGCAGG‑TCCC‑TCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGttattttatt                      <  2:1234236/93‑1 (MQ=255)

                                                                   ccc‑GGAAAAT‑CCG‑G‑GCTTCGCAGG‑TCCC‑TCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGttattttatt                      <  1:3800201/93‑1 (MQ=255)

                                                                   ccc‑GGAAAAT‑CCG‑G‑GCGTCGCAGG‑TCCC‑TCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGttattttatt                      <  1:3258482/93‑1 (MQ=255)

                                                                    cc‑GGAAAAT‑CCG‑G‑GCTTCGCAGG‑TCCC‑TCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTg                     >  1:3605119/1‑93 (MQ=255)

                                                                    cc‑GGAAAAT‑CCG‑G‑GCTTCGCAGG‑TCCC‑TCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTg                     >  1:3630673/1‑93 (MQ=255)

                                                                    cc‑GGAAAAT‑CCG‑G‑GCTTCGCAGG‑TCCC‑TCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTg                     <  2:3996342/93‑1 (MQ=255)

                                                                                    ggcttCGCAGG‑TCCC‑TCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTGTCAATTTATGAg         >  1:3440693/5‑93 (MQ=255)

                                                                                     gcttCGCAGG‑TCCC‑TCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTGTCAATTTATGAGc        >  1:1127069/4‑93 (MQ=255)

                                                                                     gcttCGCAGG‑TCCC‑TCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTGTCAATTTATGAGc        <  2:3855718/90‑1 (MQ=255)

                                                                                     gctgcGCAGG‑TCCC‑TCAAGGGGAGAAACCTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTGTCAATTTATGAGc        <  1:972014/89‑1 (MQ=255)

                                                                                      tttCGCAGG‑T‑CCGTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTTCAAAGTCATTCTCGGGGATGCTTATTTTATTGTCAATTTATGAGCg       <  2:672912/91‑1 (MQ=255)

                                                                                      cttCGCAGG‑TCCC‑TCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTGTCAATTTATGAGCg       >  1:3452303/3‑93 (MQ=255)

                                                                                       ttCGCAGG‑TCCC‑TCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTGTCAATTTATGAGCGa      >  1:377684/2‑93 (MQ=255)

                                                                                       ttCGCAGG‑TCCC‑TCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTGTCAATTTATGAGCGa      >  2:106642/2‑93 (MQ=255)

                                                                                       ttCGCAGG‑TCCC‑TCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTGTCAATTTATGAGCGa      <  2:3537196/92‑1 (MQ=255)

                                                                                       ttCGCAGG‑TCCC‑TCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTGTCAATTTATGAGCGa      >  1:3856433/2‑93 (MQ=255)

                                                                                        tCGCAGG‑TCCC‑TCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTGTCAATTTATGAGCGat     <  2:2853594/93‑1 (MQ=255)

                                                                                        tCGCAGG‑TCCC‑TCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTGTCAATTTATGAGCGat     <  1:3093857/93‑1 (MQ=255)

                                                                                         cGCAGG‑TCCC‑TCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTGTCAATTTATGAGCGata    <  2:3088012/93‑1 (MQ=255)

                                                                                          gCAGG‑TCCC‑TCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTGTCAATTTATGAGCGatat   >  1:4312881/1‑93 (MQ=255)

                                                                                          gCAGG‑TCCC‑TCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTGTCAATTTATGAGCGatat   <  1:718913/93‑1 (MQ=255)

                                                                                          gCAGG‑TCCC‑TCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTGTCAATTTATGAGCGatat   >  1:3535182/1‑93 (MQ=255)

                                                                                          gCAGG‑TCCC‑TCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTGTCAATTTATGAGCGatat   <  2:82057/93‑1 (MQ=255)

                                                                                           cAGG‑TCCC‑TCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTGTCAATTTATGAGCGATATg  <  1:2896696/93‑1 (MQ=255)

                                                                                           cAGG‑TCCC‑TCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTGTCAATTTATGAGCGATATg  <  2:1820970/93‑1 (MQ=255)

                                                                                           cAGG‑TCCC‑TCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTGTCAATTTATGAGCGATATg  >  2:3969323/1‑93 (MQ=255)

TTAGCATGATCCGCAGATGCTAACGTAATTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCC‑GGAAAAT‑CC‑‑T‑GC‑TCGCAGG‑T‑CC‑TCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTGTCAATTTATGAGCGATATG  >  NC_000913/3957469‑3957645

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 19 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

TCCGCAGATGCTAACGTAATTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCTG‑‑CT‑CGCA‑GG‑T‑CCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTGTCAATTTATGAGCGATATGGCATTGAT  >  NC_000913/3957478‑3957653

TTCGCAGATGCTAACGTAAGTGTCAGTCAGGCAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGGGCTT‑CGCA‑GG‑TCCCTCAAGGGG                                                                                       <  SRR4302535.3756935/94‑1 (MQ=60)

TCCGCAGATGCTAACGTAATTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGGGCTT‑CGCA‑GG‑TCCCTCAAGGGG                                                                                       <  SRR4302535.3756803/94‑1 (MQ=60)

  CGCAGATGCTAACGTAATTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGGGCTT‑CGCA‑GG‑TCCCTCAAGGGGAG                                                                                     <  SRR4302535.1249612/94‑1 (MQ=60)

  CGCAGATGCTAACGTAATTGTCAGTCAGGGAGTGGCTGGTACAGATATGGCAAAAGCCCGGAAAATCCGGGCTT‑CGCA‑GG‑TCCCTCAAGGGGAG                                                                                     >  SRR4302535.3317948/1‑94 (MQ=60)

  CGCAGATGCTAACGTAATTGTCAGTCAGGGAGTGGCTGGGACATATATGGCAAAAGCCCGGAAAATCCGGGCTT‑CGCA‑GG‑TCCCTCAAGGGGAG                                                                                     >  SRR4302535.2875995/1‑94 (MQ=60)

   GCAGATGCTAACGTAATTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGGGCTT‑CGCA‑GG‑TCCCTCAAGGGGAGA                                                                                    >  SRR4302535.2227627/1‑94 (MQ=60)

    CAGATGCTAACGTAATTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGGGCTT‑CGCA‑GG‑TCCCTCAAGGGGAGAA                                                                                   <  SRR4302535.3293805/94‑1 (MQ=60)

     AGATGCTAACGTAATTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGGGCTT‑CGCA‑GG‑TCCCTCAAGGGGAGAAA                                                                                  >  SRR4302535.298982/1‑94 (MQ=60)

        TGCTAACGTAATTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGGGCTT‑CGCA‑GG‑TCCCTCAAGGGGAGAAAACT                                                                               >  SRR4302535.369306/1‑94 (MQ=60)

        TGCTAACGTAATTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGGGCTT‑CGCA‑GG‑TCCCTCAAGGGGAGAAAACT                                                                               <  SRR4302535.311616/94‑1 (MQ=60)

         GCTAACGTAATTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGGAAATCCGGGCTT‑CGCA‑CG‑TCCCTCAAGGGGAGAAAACTT                                                                              >  SRR4302535.1683059/1‑94 (MQ=60)

         GCTAACGTAATTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGGGCTT‑CGCA‑GGAC‑CCTCAAGGGGAGAAAACTT                                                                              >  SRR4302535.1293711/1‑94 (MQ=60)

         GCTAACGTAATTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGGGCTT‑CGCA‑GG‑TCCCTCAAGGGGAGAAAACTT                                                                              >  SRR4302535.4225791/1‑94 (MQ=60)

         GCTAACGTAATTGTCAGTCAGGGAGTGGCTGGGACATATATGGCAAAAGCCCGGAAAATACGGGCTT‑CGCA‑GG‑TCCCTCAAGGGGAGAAAACTT                                                                              >  SRR4302535.2008279/1‑94 (MQ=60)

          CTAACGTAATTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGGGCTT‑CGCA‑GG‑TCCCTCAAGGGGAGAAAACTTA                                                                             <  SRR4302535.1887438/94‑1 (MQ=60)

           TAACGTAATGGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGGGCTT‑CGCA‑GG‑TCCCTCAAGGGGAGAAAACTTAG                                                                            <  SRR4302535.856625/94‑1 (MQ=60)

             ACGTAATTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGGGCTT‑CGCA‑GG‑TCCCTCAAGGGGAGAAAACTTAGGG                                                                          <  SRR4302535.2915547/94‑1 (MQ=60)

              CGTAATTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGGGCTT‑CGCA‑GG‑TCCCTCAAGGGGAGAAAACTTAGGGC                                                                         <  SRR4302535.789766/94‑1 (MQ=60)

               GTAATTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGGGCTT‑CGCA‑GG‑TCCCTCAAGGGGAGAAAACTTAGGGCC                                                                        >  SRR4302535.3945368/1‑94 (MQ=60)

                TAATTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGGGCTT‑CGCA‑GG‑TCCCTCAAGGGGAGAAAACTTAGGGCCT                                                                       >  SRR4302535.3353064/1‑94 (MQ=60)

                TAATTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGGGCTT‑CGCA‑GG‑TCCCTCAAGGGGAGAAAACTTAGGGCCT                                                                       <  SRR4302535.2093740/94‑1 (MQ=60)

                 AATTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGGGCTT‑CGCA‑GG‑TCCCTCAAGGGGAGAAAACTTAGGGCCTC                                                                      >  SRR4302535.566793/1‑94 (MQ=60)

                  ATTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGGGCTT‑CGCA‑GG‑TCCCTCAAGGGGAGAAAACTTAGGGCCTCT                                                                     <  SRR4302535.3036239/94‑1 (MQ=60)

                  ATTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGGGCTT‑CGCA‑GG‑TCCCTCAAGGGGAGAAAACTTAGGGCCTCT                                                                     <  SRR4302535.1027511/94‑1 (MQ=60)

                    TGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGGGCTT‑CGCA‑GG‑TCCCTCAAGGGGAGAAAACTTAGGGCCTCTAT                                                                   >  SRR4302535.4689153/1‑94 (MQ=60)

                     GTCAGTCAGGGAGTGGCTGGTACATATATTGCAAAAGACCGGAAAATCCGGGCTT‑CGCAGGG‑C‑CCTCAAGGGGAGAAAACTTAGGGCCTCTATG                                                                  >  SRR4302535.325908/1‑94 (MQ=57)

                     GTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGGGCTT‑CGCA‑GG‑TCCCTCAAGGGGAGAAAACTTAGGGCCTCTATG                                                                  <  SRR4302535.3569518/94‑1 (MQ=60)

                      TCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGGGCTT‑CGCA‑GG‑TCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGG                                                                 <  SRR4302535.542125/94‑1 (MQ=60)

                          TCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGGGCTT‑CGCA‑GG‑TCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTA                                                             <  SRR4302535.1360465/94‑1 (MQ=60)

                           CAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGGGCTT‑CGCA‑GG‑TCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTAC                                                            <  SRR4302535.2407991/94‑1 (MQ=60)

                           CAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGGGCTT‑CGCA‑GG‑TCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTAC                                                            >  SRR4302535.3028881/1‑94 (MQ=60)

                            AGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGGGCTT‑CGCA‑GG‑TCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACA                                                           <  SRR4302535.3132008/94‑1 (MQ=60)

                            AGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGGGCTT‑CGCA‑GG‑TCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACA                                                           >  SRR4302535.1510130/1‑94 (MQ=60)

                            AGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGGGCTT‑CGCA‑GG‑TCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACA                                                           <  SRR4302535.3958120/94‑1 (MQ=60)

                               GAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGGGCTT‑CGCA‑GG‑TCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAG                                                        <  SRR4302535.36438/94‑1 (MQ=60)

                               GAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGGGCTT‑CGCA‑GG‑TCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAG                                                        <  SRR4302535.1426960/94‑1 (MQ=60)

                                 GTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGGGCTT‑CGCA‑GG‑TCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTC                                                      >  SRR4302535.98904/1‑94 (MQ=60)

                                 GTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGGGCTT‑CGCA‑GG‑TCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTC                                                      <  SRR4302535.4317237/94‑1 (MQ=60)

                                 GTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGGGCTT‑CGCA‑GG‑TCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTC                                                      >  SRR4302535.2258097/1‑94 (MQ=60)

                                  TGGCTGGTACATATATGGCAAAAGCACGGAAAATCCGGGCTT‑CGCA‑GG‑TCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCA                                                     <  SRR4302535.3469552/94‑1 (MQ=60)

                                   GGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGGGCTT‑CGCA‑GG‑TCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCAT                                                    <  SRR4302535.1687257/94‑1 (MQ=60)

                                   GGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGGGCTT‑CGCA‑GG‑TCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCAT                                                    <  SRR4302535.2628030/94‑1 (MQ=60)

                                   GGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGGGCTT‑CGCA‑GG‑TCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCAT                                                    <  SRR4302535.398608/94‑1 (MQ=60)

                                       GGTACATATATGGCAAAAGCCCGGAAAATCCGGGCTT‑CGCA‑GG‑TCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATC                                                >  SRR4302535.3583971/1‑94 (MQ=60)

                                       GGTACATATATGGCAAAAGCCCGGAAAATCCGGGCTT‑CGCA‑GG‑TCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATC                                                >  SRR4302535.3309100/1‑94 (MQ=60)

                                       GGTACATATATGGCAAAAGACCGGAAAATCCGGGCTT‑CGCA‑GG‑TCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATC                                                <  SRR4302535.1584346/94‑1 (MQ=60)

                                          ACATATATGGCAAAAGCCCGGAAAATCCGGGCTT‑CGCA‑GG‑TCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGG                                             <  SRR4302535.1038522/94‑1 (MQ=60)

                                          ACATATATGGCAAAAGCCCGGAAAATCCGGGCTT‑CGCA‑GG‑TCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGG                                             >  SRR4302535.4708485/1‑94 (MQ=60)

                                           CATATATGGCAAAAGCCCGGAAAATCCGGGCTT‑CGCA‑GG‑TCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGG                                            >  SRR4302535.479815/1‑94 (MQ=60)

                                           CATATATGGCAAAAGCCCGGAAAATCCGGGCTT‑CGCA‑GG‑TCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGG                                            <  SRR4302535.3500632/94‑1 (MQ=60)

                                           CATATATGGCAAAAGCCCGGAAAATCCGGGCTT‑CGCA‑GG‑TCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGG                                            >  SRR4302535.1000298/1‑94 (MQ=60)

                                           CATATATGGCAAAAGCCCGGAAAATCCGGGCTT‑CGCA‑GG‑TCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGG                                            >  SRR4302535.4634793/1‑94 (MQ=60)

                                            ATATATGGCAAAAGCCCGGAAAATCCGGGCTT‑CGCA‑GG‑TCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGA                                           >  SRR4302535.1335191/1‑94 (MQ=60)

                                              ATATGGCAAAAGCCCGGAAAATCCGGGCTT‑CGCA‑GG‑TCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATG                                         >  SRR4302535.1307079/1‑94 (MQ=60)

                                                ATGGCAAAAGCCCGGAAAATCCGGGCTT‑CGCA‑GG‑TCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGT                                       >  SRR4302535.3823461/1‑94 (MQ=60)

                                                ATGGCAAAAGCCCGGAAAATCCGGGCTT‑CGCA‑GG‑TCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGT                                       >  SRR4302535.3164425/1‑94 (MQ=60)

                                                ATGGCAAAAGCCCGGAAAATCCGGGCTT‑CGCA‑GG‑TCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGT                                       >  SRR4302535.3853911/1‑94 (MQ=60)

                                                  GGCAAAAGCCCGGAAAATCCGGGCTT‑CGCA‑GG‑TCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTA                                     <  SRR4302535.4023845/94‑1 (MQ=60)

                                                   GCAAAAGCCCGGAAAATCCGGGCTT‑CGCA‑GG‑TCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTAT                                    >  SRR4302535.4044001/1‑94 (MQ=60)

                                                   GCAAAAGCCCGGAAAATCCGGGCTT‑CGCA‑GG‑TCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTAT                                    <  SRR4302535.4496203/94‑1 (MQ=60)

                                                    CAAAAGCCCGGAAAATCCGGGCTT‑CGCA‑GG‑TCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGCTGGTTATT                                   >  SRR4302535.2254930/1‑94 (MQ=60)

                                                    CAAAAGCCCGGAAAATCCGGGCTT‑CGCA‑GG‑TCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATT                                   >  SRR4302535.287497/1‑94 (MQ=60)

                                                    CAAAAGCCCGGAAAATCCGGGCTT‑CGCA‑GG‑TCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATT                                   >  SRR4302535.3365632/1‑94 (MQ=60)

                                                    CAAAAGCCCGGAAAATCCGGGCTT‑CGCA‑GG‑TCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATT                                   >  SRR4302535.2966436/1‑94 (MQ=60)

                                                    CAAAAGCCCGGAAAATCCGGGCTT‑CGCA‑GG‑TCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATT                                   >  SRR4302535.4402995/1‑94 (MQ=60)

                                                     AAAAGCCCGGAAAATCCGGGCTT‑CGCA‑GG‑TCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTT                                  <  SRR4302535.1963068/94‑1 (MQ=60)

                                                      AAAGCCCGGAAAATCCGGGCTT‑CGCA‑GG‑TCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTT                                 >  SRR4302535.1431463/1‑94 (MQ=60)

                                                        AGCCCGGAAAATCCGGGCTT‑CGCA‑GG‑TCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTAT                               >  SRR4302535.4056095/1‑94 (MQ=60)

                                                          CCCGGAAAATCCGGGCTT‑CGCA‑GG‑TCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTG                             >  SRR4302535.3655840/1‑94 (MQ=60)

                                                          CCCGGAAAATCCGGGCTT‑CGCA‑GG‑TCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTG                             >  SRR4302535.3630127/1‑94 (MQ=60)

                                                          CCCGGAAAATCCGGGCTT‑CGCA‑GG‑TCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTG                             <  SRR4302535.3826397/94‑1 (MQ=60)

                                                          CCCGGAAAATCCGGGCGT‑CGCA‑GG‑TCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTG                             <  SRR4302535.3281128/94‑1 (MQ=60)

                                                           CCGGAAAATCCGGGCTT‑CGCA‑GG‑TCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTGT                            >  SRR4302535.2409955/1‑94 (MQ=60)

                                                             ggaaaatcggggctT‑CGCA‑GG‑TCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTGTCA                          <  SRR4302535.858240/80‑1 (MQ=60)

                                                             ggaaaatccgggctT‑CGCA‑GG‑TCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTGTCA                          <  SRR4302535.3901562/80‑1 (MQ=60)

                                                              gaaaatccgggctT‑CGCA‑GG‑TCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTGTCAA                         >  SRR4302535.1696793/14‑94 (MQ=60)

                                                              gaaaatccgggctT‑CGCA‑GG‑TCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTGTCAA                         <  SRR4302535.4377179/81‑1 (MQ=60)

                                                               aaaatccgggctT‑CGCA‑GG‑TCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTGTCAAT                        <  SRR4302535.444853/82‑1 (MQ=60)

                                                               aaaatccgggctT‑CGCA‑GG‑TCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTGTCAAT                        <  SRR4302535.4039682/82‑1 (MQ=60)

                                                               aaaatccgggctT‑CGCA‑GG‑TCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTGTCAAT                        <  SRR4302535.1872417/82‑1 (MQ=60)

                                                                aaatccgggctT‑CGCA‑GG‑TCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTGTCAATT                       <  SRR4302535.4485946/83‑1 (MQ=60)

                                                                aaatccgggctT‑CGCA‑GG‑TCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTGTCAATT                       >  SRR4302535.2557043/12‑94 (MQ=60)

                                                                  atccgggctT‑CGCA‑GG‑TCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTGTCAATTTA                     <  SRR4302535.3756131/85‑1 (MQ=60)

                                                                   gccgggctT‑CGCA‑GG‑TCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTGTCAATTTAT                    <  SRR4302535.865585/86‑1 (MQ=60)

                                                                    ccgggctT‑CGCA‑GG‑TCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTGTCAATTTATG                   <  SRR4302535.1594667/87‑1 (MQ=60)

                                                                     cgggctT‑CGCA‑GG‑TCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTGTCAATTTATGA                  <  SRR4302535.646449/88‑1 (MQ=60)

                                                                      tccggtctgcgcaggtcCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTGTCAATTTAT                    <  SRR4302535.2627601/77‑1 (MQ=60)

                                                                      gggctT‑CGCA‑GG‑TCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTGTCAATTTATGAG                 <  SRR4302535.3177973/89‑1 (MQ=60)

                                                                      gggctT‑CGCA‑GG‑TCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTGTCAATTTATGAG                 <  SRR4302535.2723112/89‑1 (MQ=60)

                                                                      gggctT‑CGCA‑GG‑TCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTGTCAATTTATGAG                 <  SRR4302535.1417281/89‑1 (MQ=60)

                                                                      gggctT‑CGCA‑GG‑TCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTGTCAATTTATGAG                 >  SRR4302535.3464621/6‑94 (MQ=60)

                                                                       ggctT‑CGCA‑GG‑TCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTGTCAATTTATGAGC                >  SRR4302535.4180007/5‑94 (MQ=60)

                                                                       ggctT‑CGCA‑GG‑TCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTGTCAATTTATGAGC                >  SRR4302535.1135118/5‑94 (MQ=60)

                                                                       G‑‑CTGCGCA‑GG‑TCCCTCAAGGGGAGAAACCTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTGTCAATTTATGAGCG               <  SRR4302535.978911/94‑1 (MQ=60)

                                                                        gctT‑CGCA‑GG‑TCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTGTCAATTTATGAGCG               >  SRR4302535.3476299/4‑94 (MQ=60)

                                                                        gctT‑CGCA‑GG‑TCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTGTCAATTTATGAGCG               <  SRR4302535.3152455/91‑1 (MQ=60)

                                                                         ctT‑CGCA‑GG‑TCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTGTCAATTTATGAGCGT              >  SRR4302535.3887931/3‑94 (MQ=60)

                                                                         ctT‑CGCA‑GG‑TCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTGTCAATTTATGAGCGA              >  SRR4302535.3882985/3‑94 (MQ=60)

                                                                         ctT‑CGCA‑GG‑TCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTGTCAATTTATGAGCGA              >  SRR4302535.380303/3‑94 (MQ=60)

                                                                         ctT‑CGCA‑GG‑TCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTGTCAATTTATGAGCGA              >  SRR4302535.3190428/3‑94 (MQ=60)

                                                                         ctT‑CGCA‑GG‑TCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTGTCAATTTATGAGCGA              >  SRR4302535.2620133/3‑94 (MQ=60)

                                                                           T‑CGCA‑GG‑TCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTGTCAATTTATGAGCGATA            <  SRR4302535.3115404/94‑1 (MQ=60)

                                                                             CGCA‑GG‑TCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTGTCAATTTATGAGCGATAT           <  SRR4302535.1053997/94‑1 (MQ=60)

                                                                             CGCA‑GG‑TCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTGTCAATTTATGAGCGATAT           >  SRR4302535.3559725/1‑94 (MQ=60)

                                                                             CGCA‑GG‑TCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTGTCAATTTATGAGCGATAT           >  SRR4302535.4342382/1‑94 (MQ=60)

                                                                             CGCA‑GG‑TCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTGTCAATTTATGAGCGATAT           <  SRR4302535.3403381/94‑1 (MQ=60)

                                                                             CGCA‑GG‑TCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTGTCAATTTATGAGCGATAT           >  SRR4302535.361947/1‑94 (MQ=60)

                                                                              GCA‑GG‑TCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTGTCAATTTATGAGCGATATG          <  SRR4302535.723966/94‑1 (MQ=60)

                                                                               CA‑GG‑TCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTGTCAATTTATGAGCGATATGG         <  SRR4302535.2917180/94‑1 (MQ=60)

                                                                               CA‑GG‑TCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTGTCAATTTATGAGCGATATGG         <  SRR4302535.2917267/94‑1 (MQ=60)

                                                                                A‑GG‑TCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTGTCAATTTATGAGCGATATGGC        >  SRR4302535.980244/1‑94 (MQ=60)

                                                                                A‑GG‑TCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTGTCAATTTATGAGCGATATGGC        <  SRR4302535.1196242/94‑1 (MQ=60)

                                                                                   G‑C‑CCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTAGCGGGGATGGTTATTTTATTGTCAATTTATGAGCGATATGGCATT     <  SRR4302535.1603020/94‑1 (MQ=60)

                                                                                    gtcCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTGTCAATTTATGAGCGATATGGCAT      <  SRR4302535.2421340/91‑1 (MQ=60)

                                                                                    gtcCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTGTCAATTTATGAGCGATATGGCAT      >  SRR4302535.3091661/4‑94 (MQ=60)

                                                                                    gtcCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTGTCAATTTATGAGCGATATGGCAT      >  SRR4302535.760001/4‑94 (MQ=60)

                                                                                     tcCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTGTCAATTTATGAGCGATATGGCATT     <  SRR4302535.4553870/92‑1 (MQ=60)

                                                                                     C‑CCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTGTCAATTTATGAGCGATATGGCATTG    <  SRR4302535.4663504/94‑1 (MQ=60)

                                                                                     C‑CCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTGTCAATTTATGAGCGATATGGCATTG    >  SRR4302535.3904756/1‑94 (MQ=60)

                                                                                     C‑CCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTGTCAATTTATGAGCGATATGGCATTG    <  SRR4302535.395069/94‑1 (MQ=60)

                                                                                       CCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTGTCAATTTATGAGCGATATGGCATTGA   >  SRR4302535.3005961/1‑94 (MQ=60)

                                                                                       CCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTGTCAATTTATGAGCGATATGGCATTGA   >  SRR4302535.29180/1‑94 (MQ=60)

                                                                                        CTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTGTCAATTTATGAGCGATATGGCATTGAT  >  SRR4302535.3935635/1‑94 (MQ=60)

                                                                                        CTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTGTCAATTTATGAGCGATATGGCATTGAT  <  SRR4302535.2950123/94‑1 (MQ=60)

                                                                                        CTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTGTCAATTTATGAGCGATATGGCATTGAT  >  SRR4302535.2480428/1‑94 (MQ=60)

                                                                                        CTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTGTCAATTTATGAGCGATATGGCATTGAT  <  SRR4302535.1951511/94‑1 (MQ=60)

TCCGCAGATGCTAACGTAATTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCTG‑‑CT‑CGCA‑GG‑T‑CCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTGTCAATTTATGAGCGATATGGCATTGAT  >  NC_000913/3957478‑3957653

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 8 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`