Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 1873 183.1 9376148 98.2% 9207377 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,964,041 A→G T4A (ACC→GCC)  rho → transcription termination factor Rho

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,964,0410AG100.0% 771.6 / NA 242T4A (ACC→GCC) rhotranscription termination factor Rho
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (112/130);  total (112/130)

Only 100 of 246 total aligned reads displayed.

TAAACAGGCATGGATGATCCTGCCATACCATTCACAACATTAAGTTCGAGATTTACCCCAAGTTTAAGAACTCACACCACTATGAATCTTACCGAATTAAAGAATACGCCGGTTTCTGAGCTGATCACTCTCGGCGAAAATATGGGGCTGGAAAACCTGGCTCGTATGCGTAAGCAGGAC  >  NC_000913/3963951‑3964130
                                                                                          |                                                                                         
   aCAGGCATGGATGATCCTGCCATACCATTCACAACATTAAGTTCGAGATTTACCCCAAGTTTAAGAACTCACACCACTATGAATCTTGCCGaa                                                                                      <  2:1719989/93‑1 (MQ=255)
   aCAGGCATGGATGATCCTGCCATACCATTCACAACATTAAGTTCGAGATTTACCCCAAGTTTAAGAACTCACACCACTATGAATCTTGCCGaa                                                                                      <  2:4432254/93‑1 (MQ=255)
     aGGCATGGATGATCCTGCCATACCATTCACAACATTAAGTTCGAGATTTACCCCAAGTTTAAGAACTCACACCACTATGAATCTTGCCGAAtt                                                                                    <  1:3689990/93‑1 (MQ=255)
       gCATGGATGATCCTGCCATACCATTCACAACATTAAGTTCGAGATTTACCCCAAGTTTAAGAACTCACACCACTATGAATCTTGCCGAATTaa                                                                                  <  1:1962798/93‑1 (MQ=255)
        cATGGATGATCCTGCCATACCATTCACAACATTAAGTTCGAGATTTACCCCAAGTTTAAGAACTCACACCACTATGAATCTTGCCGAATTaaa                                                                                 <  2:1598233/93‑1 (MQ=255)
        cATGGATGATCCTGCCATACCATTCACAACATTAAGTTCGAGATTTACCCCAAGTTTAAGAACTCACACCACTATGAATCTTGCCGAATTaaa                                                                                 <  2:780749/93‑1 (MQ=255)
        cATGGATGATCCTGCCATACCATTCACAACATTAAGTTCGAGATTTACCCCAAGTTTAAGAACTCACACCACTATGAATCTTGCCGAATTaaa                                                                                 <  1:4548290/93‑1 (MQ=255)
          tGGATGATCCTGCCATACCATTCACAACATTAAGTTCGAGATTTACCCCAAGTTTAAGAACTCACACCACTATGAATCTTGCCGAATTAAAGa                                                                               >  1:3582315/1‑93 (MQ=255)
          tGGATGATCCTGCCATACCATTCACAACATTAAGTTCGAGATTTACCCCAAGTTTAAGAACTCACACCACTATGAATCTTGCCGAATTAAAGa                                                                               <  1:4047701/93‑1 (MQ=255)
          tGGATAATCCTGCCATACCATTCACAACATTAAGTTCGAGATTTACCCCAAGTTTAAGAACTCACACCACTATGAATCTTGCCGAATTAAAGa                                                                               <  2:349915/93‑1 (MQ=255)
            gatgttCCTGCCATACCATTCACAACATTAAGTTCGAGATTTACCCCAAGTTTAAGACCTCACACCACTATGAATCTTGCCGAATTAAAGAAt                                                                             <  1:3968324/93‑1 (MQ=255)
             atgatCCTGCAATACCATTCACAACATTAAGTTCGAGATTTACCCCAAGTTTAAGAACTCACACCACTATGAATCTTGCCGAATTAAAGAAtt                                                                            <  2:3874772/93‑2 (MQ=255)
             atgacCCTGCGATCCCATTCACAACATTAAGTTCGAGATTTACCCCAAGTTTAAGAACTCACACCACTATGAATCTTGCCGAATTAAAGAATa                                                                            <  2:875498/93‑1 (MQ=255)
              tgatCCTGCCATACCATTCACAACATTAAGTTCGAGATTTACCCCAAGTTTAAGAACTCACACCACTATGAATCTTGCCGAATTAAAGAATAc                                                                           >  1:536675/1‑93 (MQ=255)
               gatCCTGCCATACCATTCACAACATTAAGTTCGAGATTTACCCCAAGTTTAAGAACTCACACCACTATGAATCTTGCCGAATTAAAGAATACg                                                                          >  1:2706250/1‑93 (MQ=255)
               gatCCTGCCATACCATTCACAACATTAAGTTCGAGATTTACCCCAAGTTTAAGAACTCACACCACTATGAATCTTGCCGAATTAAAGAATACg                                                                          >  1:685045/1‑93 (MQ=255)
                acccTCCCATACCATTCACAACATTAAGTTCGAGATTTACCCCAAGTTTAAGAACTCACACCACTATGAATCTTGCCGAATTAAAGAATACGc                                                                         <  2:2526828/91‑1 (MQ=255)
                  ccTGCCATACCATTCACAACATTAAGTTCGAGATTTACCCCAAGTTTAAGAACTCACACCACTATGAATCTTGCCGAATTAAAGAATACGCCg                                                                       <  2:3460297/93‑1 (MQ=255)
                   cTGCCATACCATTCACAACATTAAGTTCGAGATTTACCCCAAGTTTAAGAACTCACACCACTATGAATCTTGCCGAATTAAAGAATACGCCgg                                                                      <  2:2087586/93‑1 (MQ=255)
                   cTGCCATACCATTCACAACATTAAGTTCGAGATTTACCCCAAGTTTAAGAACTCACACCACTATGAATCTTGCCGAATTAAAGAATACGCCgg                                                                      >  1:1555260/1‑93 (MQ=255)
                       cATACCATTCACAACATTAAGTTCGAGATTTACCCCAAGTTTAAGAACTCACACCACTATGAATCTTGCCGAATTAAAGAATACGCCGGTTTc                                                                  <  1:2710292/93‑1 (MQ=255)
                       cATACCATTCACAACATTAAGTTCGAGATTTACCCCAAGTTTAAGAACTCACACCACTATGAATCTTGCCGAATTAAAGAATACGCCGGTTTc                                                                  <  1:2120117/93‑1 (MQ=255)
                        aTACCATTCACAACATTAAGTTCGAGATTTACCCCAAGTTTAAGAACTCACACCACTATGAATCTTGCCGAATTAAAGAATACGCCGGTTTCt                                                                 >  2:1523493/1‑93 (MQ=255)
                        aTACCATTCACAACATTAAGTTCGAGATTTACCCCAAGTTTAAGAACTCACACCACTATGAATCTTGCCGAATTAAAGAATACGCCGGTTTCt                                                                 >  1:2070007/1‑93 (MQ=255)
                         tACCATTCACAACATTAAGTTCGAGATTTACCCCAAGTTTAAGAACTCACACCACTATGAATCTTGCCGAATTAAAGAATACGCCGGTTTCTg                                                                <  1:3379670/93‑1 (MQ=255)
                         tACCATTCACAACATTAAGTTCGAGATTTACCCCAAGTTTAAGAACTCACACCACTATGAATCTTGCCGAATTAAAGAATACGCCGGTTTCTg                                                                <  2:1137574/93‑1 (MQ=255)
                           ccATTCACAACATTAAGTTCGAGATTTACCCCAAGTTTAAGAACTCACACCACTATGAATCTTGCCGAATTAAAGAATACGCCGGTTTCTGAg                                                              >  2:4080141/1‑93 (MQ=255)
                            cATTCACAACATTAAGTTCGAGATTTACCCCAAGTTTAAGAACTCACACCACTATGAATCTTGCCGAATTAAAGAATACGCCGGTTTCTGAGc                                                             >  1:2157624/1‑93 (MQ=255)
                            cATTCACAACATTAAGTTCGAGATTTACCCCAAGTTTAAGAACTCACACCACTATGAATCTTGCCGAATTAAAGAATACGCCGGTTTCTGAGc                                                             <  2:475519/93‑1 (MQ=255)
                             aTTCACAACATTAAGTTCGAGATTTACCCCAAGTTTAAGAACTCACACCACTATGAATCTTGCCGAATTAAAGAATACGCCGGTTTCTGAGCt                                                            >  1:100924/1‑93 (MQ=255)
                             aTTCACAACATTAAGTTCGAGATTTACCCCAAGTTTAAGAACTCACACCACTATGAATCTTGCCGAATTAAAGAATACGCCGGTTTCTGAGCt                                                            >  2:342789/1‑93 (MQ=255)
                             aTTCACAACATTAAGTTCGAGATTTACCCCAAGTTTAAGAACTCACACCACTATGAATCTTGCCGAATTAAAGAATACGCCGGTTTCTGAGCt                                                            >  1:432031/1‑93 (MQ=255)
                               tCACAACATTAAGTTCGAGATTTACCCCAAGTTTAAGAACTCACACCACTATGAATCTTGCCGAATTAAAGAATACGCCGGTTTCTGAGCTGa                                                          <  2:540186/93‑1 (MQ=255)
                               tCACAACATTAAGTTCGAGATTTACCCCAAGTTTAAGAACTCACACCACTATGAATCTTGCCGAATTAAAGAATACGCCGGTTTCTGAGCTGa                                                          >  2:2917347/1‑93 (MQ=255)
                                cacaACATTAAGTTCGAGATTTACCCCAAGTTTAAGAACTCACACCACTATGAATCTTGCCGAATTAAAGAATCCGCCGGTTTCTGAGCTGAt                                                         <  2:4526044/93‑1 (MQ=255)
                                 acaacaTTAAGTTCGAGATTTACCCCAAGTTTAAGAACTCACACCACTATGAATCTTGCCGAATTAAAGAATACGCCGGTTTCTGAGCTGATc                                                        <  1:3710812/93‑1 (MQ=255)
                                 acaacaTTAAGTTCGAGATTTACCCCAAGTTTAAGAACTCACACCACTATGAATCTTGCCGAATTAAAGAATACGCCGGTTTCTGAGCTGATc                                                        <  2:3673481/93‑1 (MQ=255)
                                 acaacaTTAAGTTCGAGATTTACCCCAAGTTTAAGAACTCACACCACTATGAATCTTGCCGAATTAAAGAATACGCCGGTTTCTGAGCTGATc                                                        >  2:65977/1‑93 (MQ=255)
                                 acaacaTTAAGTTCGAGATTTACCCCAAGTTTAAGAACTCACACCACTATGAATCTTGCCGAATTAAAGAATACGCCGGTTTCTGAGCTGATc                                                        <  1:3489878/93‑1 (MQ=255)
                                   aacaTTAAGTTCGAGATTTACCCCAAGTTTAAGAACTCACACCACTATGAATCTTGCCGAATTAAAGAATACGCCGGTTTCTGAGCTGATCAc                                                      <  1:2474694/93‑1 (MQ=255)
                                     caTTAAGTTCGAGATTTACCCCAAGTTTAAGAACTCACACCACTATGAATCTTGCCGAATTAAAGAATACGCCGGTTTCTGAGCTGATCActc                                                    <  2:1444773/93‑1 (MQ=255)
                                      aTTAAGTTCGAGATTTACCCCAAGTTTAAGAACTCACACCACTATGAATCTTGCCGAATTAAAGAATACGCCGGTTTCTGAGCTGATCActct                                                   <  1:3838986/93‑1 (MQ=255)
                                      aTTAAGTTCGAGATTTACCCCAAGTTTAAGAACTCACACCACTATGAATCTTGCCGAATTAAAGAATACGCCGGTTTCTGAGCTGATCActct                                                   <  2:682543/93‑1 (MQ=255)
                                      aTTAAGTTCGAGATTTACCCCAAGTTTAAGAACTCACACCACTATGAATCTTGCCGAATTAAAGAATACGCCGGTTTCTGAGATGATCActct                                                   >  2:182565/1‑93 (MQ=255)
                                       ttAAGTTCGAGATTTACCCCAAGTTTAAGAACTCACACCACTATGAATCTTGCCGAATTAAAGAATACGCCGGTTTCTGAGCTGATCACtctc                                                  >  1:834713/1‑93 (MQ=255)
                                         aaGTTCGAGATTTACCCCAAGTTTAAGAACTCACACCACTATGAATCTTGCCGAATTAAAGAATACGCCGGTTTCTGAGCTGATCACTCTCgg                                                >  2:4506684/1‑93 (MQ=255)
                                         aaGTTCGAGATTTACCCCAAGTTTAAGAACTCACACCACTATGAATCTTGCCGAATTAAAGAATACGCCGGTTTCTGAGCTGATCACTCTCgg                                                <  1:2578883/93‑1 (MQ=255)
                                              cGAGATTTACCCCAAGTTTAAGAACTCACACCACTATGAATCTTGCCGAATTAAAGAATACGCCGGTTTCTGAGCTGATCACTCTCGGCGaaa                                           <  2:2709830/93‑1 (MQ=255)
                                                 gaTTTACCCCAAGTTTAAGAACTCACACCACTATGAATCTTGCCGAATTAAAGAATACGCCGGTTTCTGAGCTGATCACTCTCGGCGAAAata                                        >  2:2245255/1‑93 (MQ=255)
                                                  aTTTACCCCAAGTTTAAGAACTCACACCACTATGAATCTTGCCGAATTAAAGAATACGCCGGTTTCTGAGCTGATCACTCTCGGCGAAAatat                                       <  1:917001/93‑1 (MQ=255)
                                                   tttACCCCAAGTTTAAGAACTCACACCACTATGAATCTTGCCGAATTAAAGAATACGCCGGTTTCTGAGCTGATCACTCTCGGCGAAAATATg                                      >  2:3844284/1‑93 (MQ=255)
                                                   tttACCCCAAGTTTAAGAACTCACACCACTATGAATCTTGCCGAATTAAAGAATACGCCGGTTTCTGAGCTGATCACTCTCGGCGAAAATATg                                      >  1:3201645/1‑93 (MQ=255)
                                                    ttACCCCAAGTTTAAGAACTCACACCACTATGAATCTTGCCGAATTAAAGAATACGCCGGTTTCTGAGCTGATCACTCTCGGCGAAAATATgg                                     >  1:1295636/1‑93 (MQ=255)
                                                    ttACCCCAAGTTTAAGAACTCACACCACTATGAATCTTGCCGAATTAAAGAATACGCCGGTTTCTGAGCTGATCACTCTCGGCGAAAATATgg                                     <  1:4282189/93‑1 (MQ=255)
                                                     tACCCCAAGTTTAAGAACTCACACCACTATGAATCTTGCCGAATTAAAGAATACGCCGGTTTCTGAGCTGATCACTCTCGGCGAAAATATggg                                    <  1:1203226/93‑1 (MQ=255)
                                                     tACCCCAAGTTTAAGAACTCACACCACTATGAATCTTGCCGAATTAAAGAATACGCCGGTTTCTGAGCTGATCACTCTCGGCGAAAATATggg                                    <  2:4303141/93‑1 (MQ=255)
                                                     tACCCCAAGTTTAAGAAATCACACCACTATGAATCTTGCCGAATTAAAGAATACGCCGGTTTCTGAGCTGATCACTCTCGGCGAAAATATggg                                    <  1:1403474/93‑1 (MQ=255)
                                                       ccccAAGTTTAAGAACTCACACCACTATGAATCTTGCCGAATTAAAGAATACGCCGGTTTCTGAGCTGATCACTCTCGGCGAAAATATGGGGc                                  >  2:3444088/1‑93 (MQ=255)
                                                       ccccAAGTTTAAGAACTCACACCACTATGAATCTTGCCGAATTAAAGAATACGCCGGTTTCTGAGCTGATCACTCTCGGCGAAAATATGGGGc                                  >  2:3162561/1‑93 (MQ=255)
                                                        cccAAGTTTAAGAACTCACACCACTATGAATCTTGCCGAATTAAAGAATACGCCGGTTTCTGAGCTGATCACTCTCGGCGAAAATATGGGGCt                                 >  1:393556/1‑93 (MQ=255)
                                                        cccAAGTTTAAGAACTCACACCACTATGAATCTTGCCGAATTAAAGAATACGCCGGTTTCTGAGCTGATCACTCTCGGCGAAAATATGGGGCt                                 >  2:936483/1‑93 (MQ=255)
                                                        cccAAGTTTAAGAACTCACACCACTATGAATCTTGCCGAATTAAAGAATACGCCGGTTTCTGAGCTGATCACTCTCGGCGAAAATATGGGGCt                                 >  1:1520133/1‑93 (MQ=255)
                                                         ccAAGTTTAAGAACTCACACCACTATGAATCTTGCCGAATTAAAGAATACGCCGGTTTCTGAGCTGATCACTCTCGGCGAAAATATGGGGCTg                                <  1:3280007/93‑1 (MQ=255)
                                                         ccAAGTTTAAGAACTCACACCACTATGAATCTTGCCGAATTAAAGAATACGCCGGTTTCTGAGCTGATCACTCTCGGCGAAAATATGGGGCTg                                >  2:593996/1‑93 (MQ=255)
                                                          cAAGTTTAAGAACTCACACCACTATGAATCTTGCCGAATTAAAGAATACGCCGGTTTCTGAGCTGATCACTCTCGGCGAAAATATGGGGCTgg                               <  1:511741/93‑1 (MQ=255)
                                                           aaGTATAAGAAATCACACCCATGTGAAGCTTGCCGAATTAAAGAATACGCCGGTTTCTGAGCTGATCCCGCTCGGCGAAAATATGGGGCTGGa                              <  2:3080606/93‑1 (MQ=255)
                                                            aGTTTAAGAACTCACACCACTATGAATCTTGCCGAATTAAAGAATACGCCGGTTTCTGAGCTGATCACTCTCGGCGAAAATATGGGGCTGGaa                             >  1:1793142/1‑93 (MQ=255)
                                                              tttAAGAACTCACACCACTATGAATCTTGCCGAATTAAAGAATACGCCGGTTTCTGAGCTGATCACTCTCGGCGAAAATATGGGGCTGGaaaa                           >  2:2309384/1‑93 (MQ=255)
                                                               ttAAGAACTCACACCACTATGAATCTTGCCGAATTAAAGAATACGCCGGTTTCTGAGCTGATCACTCTCGGCGAAAATATGGGGCTGGAAAAc                          <  1:1787991/93‑1 (MQ=255)
                                                                tAAGAACTCACACCACTATGAATCTTGCCGAATTAAAGAATACGCCGGTTTCTGAGCTGATCACTCTCGGCGAAAATATGGGGCTGGAAATcc                         <  2:2570238/93‑1 (MQ=255)
                                                                tAAGAACTCACACCACTATGAATCTTGCCGAATTAAAGAATACGCCGGTTTCTGAGCTGATCACTCTCGGCGAAAATATGGGGCTGGAAAAcc                         <  2:1027088/93‑1 (MQ=255)
                                                                tAAGAACTCACACCACTATGAATCTTGCCGAATTAAAGAATACGCCGGTTTCTGAGCTGATCACTCTCGGCGAAAATATGGGGCTGGAAAAcc                         <  1:24242/93‑1 (MQ=255)
                                                                tAAGAACTCACACCACTATGAATCTTGCCGAATTAAAGAATACGCCGGTTTCTGAGCTGATCACTCTCGGCGAAAATATGGGGCTGGAAAAcc                         >  2:135171/1‑93 (MQ=255)
                                                                tAAGAACTCACACCACTATGAATCTTGCCGAATTAAAGAATACGCCGGTTTCTGAGCTGATCACTCTCGGCGAAAATATGGGGCTGGAAAAcc                         >  1:4177171/1‑93 (MQ=255)
                                                                 aaGAACTCACACCACTATGAATCTTGCCGAATTAAAGAATACGCCGGTTTCTGAGCTGATCACTCTCGGCGAAAATATGGGGCTGGAAAACCt                        >  2:1959626/1‑93 (MQ=255)
                                                                  aGAACTCACACCACTATGAATCTTGCCGAATTAAAGAATACGCCGGTTTCTGAGCTGATCACTCTCGGCGAAAATATGGGGCTGGAAAACCTg                       >  1:1883017/1‑93 (MQ=255)
                                                                  aGAACTCACACCACTATGAATCTTGCCGAATTAAAGAATACGCCGGTTTCTGAGCTGATCACTCTCGGCGAAAATATGGGGCTGGAAAACCTg                       >  1:3046671/1‑93 (MQ=255)
                                                                  aGAACTCACACCACTATGAATCTTGCCGAATTAAAGAATACGCCGGTTTCTGAGCTGATCACTCTCGGCGAAAATATGGGGCTGGAAAACCTg                       <  2:3662320/93‑1 (MQ=255)
                                                                  aGAACTCACACCACTATGAATCTTGCCGAATTAAAGAATACGCCGGTTTCTGAGCTGATCACTCTCGGCGAAAATATGGGGCTGGAAAACCTg                       <  1:4452124/93‑1 (MQ=255)
                                                                   gAACTCACACCACTATGAATCTTGCCGAATTAAAGAATACGCCGGTTTCTGAGCTGATCACTCTCGGCGAAAATATGGGGCTGGAAAACCTgg                      >  2:2162954/1‑93 (MQ=255)
                                                                   gAACTCACACCACTATGAATCTTGCCGAATTAAAGAATACGCCGGTTTCTGAGCTGATCACTCTCGGCGAAAATATGGGGCTGGAAAACCTgg                      >  2:1321478/1‑93 (MQ=255)
                                                                    aaCTCACACCACTATGAATCTTGCCGAATTAAAGAATACGCCGGTTTCTGAGCTGATCACTCTCGGCGAAAATATGGGGCTGGAAAACCTGGc                     >  1:2329943/1‑93 (MQ=255)
                                                                    aaCTCACACCACTATGAATCTTGCCGAATTAAAGAAGACGCCGGTTTCTGAGCTGATCACTCTCGGCGAAAATATGGGGCTGGAAAACCTGGc                     <  1:3580149/93‑1 (MQ=255)
                                                                     aCTCACACCACTATGAATCTTGCCGAATTAAAGAATACGCCGGTTTCTGAGCTGATCACTCTCGGCGAAAATATGGGGCTGGAAAACCTGGCt                    <  1:1043667/93‑1 (MQ=255)
                                                                     aCTCACACCACTATGAATCTTGCCGAATTAAAGAATACGCCGGTTTCTGAGCTGATCACTCTCGGCGAAAATATGGGGCTGGAAAACCTGGCt                    >  1:4582436/1‑93 (MQ=255)
                                                                     aCTCACACCACTATGAATCTTGCCGAATTAAAGAATACGCCGGTTTCTGAGCTGATCACTCTCGGCGAAAATATGGGGCTGGAAAACCTGGCt                    <  1:2228010/93‑1 (MQ=255)
                                                                     aCTCACACCACTATGAATCTTGCCGAATTAAAGAATACGCCGGTTTCTGAGCTGATCACTCTCGGCGAAAATATGGGGCTGGAAAACCTGGCt                    <  1:2113293/93‑1 (MQ=255)
                                                                      cTCACACCACTATGAATCTTGCCGAATTAAAGAATACGCCGGTTTCTGAGCTGATCACTCTCGGCGAAAATATGGGGCTGGAAAACCTGGCTc                   >  2:1424953/1‑93 (MQ=255)
                                                                       tCACACCACTATGAATCTTGCCGAATTAAAGAATACGCCGGTTTCTGAGCTGATCACTCTCGGCGAAAATATGGGGCTGGAAAACCTGGCTCg                  <  1:1375014/93‑1 (MQ=255)
                                                                         acacCACTATGAATCTTGCCGAATTAAAGAATACGCCGGTTTCTGAGCTGATCACTCTCGGCGAAAATATGGGGCTGGAAAACCTGGCTCGTa                <  2:323580/93‑1 (MQ=255)
                                                                         acacCACTATGAATCTTGCCGAATTAAAGAATACGCCGGTTTCTGAGCTGATCACTCTCGGCGAAAATATGGGGCTGGAAAACCTGGCTCGTa                <  2:974878/93‑1 (MQ=255)
                                                                           accacTATGAATCTTGCCGAATTAAAGAATACGCCGGTTTCTGAGCTGATCACTCTCGGCGAAAATATGGGGCTGGAAAACCTGGCTCGTATg              >  1:1091245/1‑93 (MQ=255)
                                                                            ccacTATGAATCTTGCCGAATTAAAGAATACGCCGGTTTCTGAGCTGATCACTCTCGGCGAAAATATGGGGCTGGAAAACCTGGCTCGTATga             <  1:3324123/93‑2 (MQ=255)
                                                                            ccacTATGAATCTTGCCGAATTAAAGAATACGCCGGTTTCTGAGCTGATCACTCTCGGCGAAAATATGGGGCTGGAAAACCTGGCTCGTATGc             >  1:4624396/1‑93 (MQ=255)
                                                                                tATGAATCTTGCCGAATTAAAGAATACGCCGGTTTCTGAGCTGATCACTCTCGGCGAAAATATGGGGCTGGAAAACCTGGCTCGTATGCGTaa         <  1:2753189/93‑1 (MQ=255)
                                                                                   gAATCTTGCCGAATTAAAGAATACGCCGGTTTCTGAGCTGATCACTCTCGGCGAAAATATGGGGCTGGAAAACCTGGCTCGTATGCGTAAGCa      >  1:1406919/1‑93 (MQ=255)
                                                                                    aaTCTTGCCGAATTAAAGAATACGCCGGTTTCTGAGCTGATCACTCTCGGCGAAAATATGGGGCTGGAAAACCTGGCTCGTATGCGTAAGCAg     <  2:1890446/93‑1 (MQ=255)
                                                                                    aaTCTTGCCGAATTAAAGAATACGCCGGTTTCTGAGCTGATCACTCTCGGCGAAAATATGGGGCTGGAAAACCTGGCTCGTATGCGTAAGCAg     >  2:1785178/1‑93 (MQ=255)
                                                                                    aaTCTTGCCGAATTAAAGAATACGCCGGTTTCTGAGCTGATCACTCTCGGCGAAAATATGGGGCTGGAAAACCTGGCTCGTATGCGTAAGCAg     <  1:1193095/93‑1 (MQ=255)
                                                                                     aTCTTGCCGAATTAAAGAATACGCCGGTTTCTGAGCTGATCACTCTCGGCGAAAATATGGGGCTGGAAAACCTGGCTCGTATGCGTAAGCAgg    >  2:559921/1‑93 (MQ=255)
                                                                                          |                                                                                         
TAAACAGGCATGGATGATCCTGCCATACCATTCACAACATTAAGTTCGAGATTTACCCCAAGTTTAAGAACTCACACCACTATGAATCTTACCGAATTAAAGAATACGCCGGTTTCTGAGCTGATCACTCTCGGCGAAAATATGGGGCTGGAAAACCTGGCTCGTATGCGTAAGCAGGAC  >  NC_000913/3963951‑3964130

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CCGTAAACAGGCATGGATGATCCTGCCATACCATTCACAACATTAAGTTCGAGATTTACCCCAAGTTTAAGAACTCACACCACTATGAATCTTACCGAATTAAAGAATACGCCGGTTTCTGAGCTGATCACTCTCGGCGAAAATATGGGGCTGGAAAACCTGGCTCGTATGCGTAAGCAGGACATTA  >  NC_000913/3963948‑3964134
                                                                                             |                                                                                             
CCGTAAACAGGCATGGATGATCCTGCCATACCATTCACAACATTAAGTTCGAGATTTACCCCAAGTTTAAGAACTCACACCACTATGAATCTTG                                                                                               <  SRR4302535.4545928/94‑1 (MQ=60)
CCGTAAACAGGCATGGATGATCCTGCCATACCATTCACAACATTAAGTTCGAGATTTACCCCAAGTTTAAGAACTCACACCACTATGAATCTTC                                                                                               >  SRR4302535.3953561/1‑94 (MQ=60)
 CGTAAACAGGCATGGATGATCCTGCCATACCATTCACAACATTAAGTTCGAGATTTACCCCAAGTTTAAGAACTCACACCACTATGAATCTTGC                                                                                              <  SRR4302535.3987650/94‑1 (MQ=60)
 CGTAAACAGGCATGGATGATCCTGCCATACCATTCACAACATTAAGTTCGAGATTTACCCCAAGTTTAAGAACTCACACCACTATGAATCTTGC                                                                                              >  SRR4302535.2108353/1‑94 (MQ=60)
  GTAAACAGGCATGGATGATCCTGCCATACCATTCACAACATTAAGTTCGAGATTTACCCCAAGTTTAAGAACTCACACCACTATGAATCTTGCC                                                                                             <  SRR4302535.2625175/94‑1 (MQ=60)
       CAGTCAGGGATGACCCTGCCATACCATGCACAACATTAAGTTCGAGATTTACCCCAAGTTTAAGAACTCACACCACTATGAATCTTGCCGAATT                                                                                        <  SRR4302535.564803/94‑1 (MQ=60)
       CAGGCATGGATGATCCTGCCATACCATTCACAACATTAAGTTCGAGATTTACCCCAAGTTTAAGAACTCACACCACTATGAATCTTGCCGAATT                                                                                        >  SRR4302535.376278/1‑94 (MQ=60)
        AGGCATGGATGATCCTGCCATACCATTCACAACATTAAGTTCGAGATTTACCCCAAGTTTAAGAACTCACACCACTATGAATCTTGCCGAATTA                                                                                       <  SRR4302535.3715529/94‑1 (MQ=60)
          GCATGGATGATCCTGCCATACCATTCACAACATTAAGTTCGAGATTTACCCCAAGTTTAAGAACTCACACCACTATGAATCTTGCCGAATTAAA                                                                                     <  SRR4302535.1976952/94‑1 (MQ=60)
          GCATGAATGATCCTGCCATACCATTCACAACATTAAGTTCGAGATTTACCCCAAGTTTAAGAACTCACACCACTATGAATCTTGCCGAATTAAA                                                                                     <  SRR4302535.1415390/94‑1 (MQ=60)
           CATGGATGATCCTGCCATACCATTCACAACATTAAGTTCGAGATTTACCCCAAGTTTAAGAACTCACACCACTATGAATCTTGCCGAATTAAAG                                                                                    <  SRR4302535.4579387/94‑1 (MQ=60)
           CATGGATGATCCTGCCATACCATTCACAACATTAAGTTCGAGATTTACCCCAAGTTTAAGAACTCACACCACTATGAATCTTGCCGAATTAAAG                                                                                    <  SRR4302535.3692415/94‑1 (MQ=60)
           CATGGATGATCCTGCCATACCATTCACAACATTAAGTTCGAGATTTACCCCAAGTTTAAGAACTCACACCACTATGAATCTTGCCGAATTAAAG                                                                                    <  SRR4302535.3388109/94‑1 (MQ=60)
            ATGGATGATCCTGCCATACCATTCACAACATTAAGTTCGAGATTTACCCCAAGTTTAAGAACTCACACCACTATGAATCTTGCCGAATTAAAGA                                                                                   <  SRR4302535.772269/94‑1 (MQ=60)
            ATGGATGATCCTGCCATACCATTCACAACATTAAGTTCGAGATTTACCCCAAGTTTAAGAACTCACACCACTATGAATCTTGCCGAATTAAAGA                                                                                   >  SRR4302535.3607162/1‑94 (MQ=60)
            AAGGATGACCCTGCCATACCATTCACAACATTAAGTTCGAGATTTGCCCCAAGTTTAAGAACTCACACCACTATGAATCTTGCCGAATTAAAGA                                                                                   <  SRR4302535.2163857/94‑1 (MQ=60)
             TGGATGATCCTGCCATACCATTCACAACATTAAGTTCGAGATTTACCCCAAGTTTAAGAACTCACACCACTATGAATCTTGCCGAATTAAAGAA                                                                                  <  SRR4302535.4075409/94‑1 (MQ=60)
              GGATGATCCTGCCATACCATTCACAACATTAAGTTCGAGATTTACCCCAAGTTTAAGAACTCACACCACTATGAATCTTGCCGAATTAAAGAAT                                                                                 >  SRR4302535.4216323/1‑94 (MQ=60)
               GATGTTCCTGCCATACCATTCACAACATTAAGTTCGAGATTTACCCCAAGTTTAAGACCTCACACCACTATGAATCTTGCCGAATTAAAGAATA                                                                                <  SRR4302535.3995427/94‑1 (MQ=60)
               GATGATCCTGCCATACCATTCACAACATTAAGTTCGAGATTTACCCCAAGTTTAAGAACTCACACCACTATGAATCTTGCCGAATTAAAGAATA                                                                                <  SRR4302535.4602453/94‑1 (MQ=60)
                ATGATCCTGCCATACCATTCACAACATTAAGTTCGAGATTTACCCCAAGTTTAAGAACTCACACCACTATGAATCTTGCCGAATTAAAGAATAC                                                                               >  SRR4302535.540425/1‑94 (MQ=60)
                ATGATCCTGCCATACCATTCACAACATTAAGTTCGAGATTTACCCCAAGTTTAAGAACTCACACCACTATGAATCTTGCCGAATTAAAGAATAC                                                                               <  SRR4302535.2108447/94‑1 (MQ=60)
                ATGATCCTGCCATACCATTCACAACATTAAGTTCGAGATTTACCCCAAGTTTAAGAACTCACACCACTATGAATCTTGCCGAATTAAAGAATAC                                                                               >  SRR4302535.484235/1‑94 (MQ=60)
                 TGATCCTGCCATACCATTCACAACATTAAGTTCGAGATTTACCCCAAGTTTAAGAACTCACACCACTATGAATCTTGCCGAATTAAAGAATACG                                                                              >  SRR4302535.2725589/1‑94 (MQ=60)
                 TGATCCTGCCATACCATTCACAACATTAAGTTCGAGATTTACCCCAAGTTTAAGAACTCACACCACTATGAATCTTGCCGAATTAAAGAATACG                                                                              >  SRR4302535.689846/1‑94 (MQ=60)
                  GATCCTGCCATACCATTCACAACATTAAGTTCGAGATTTACCCCAAGTTTAAGAACTCACACCACTATGAATCTTGCCGAATTAAAGAATACGC                                                                             >  SRR4302535.1938378/1‑94 (MQ=60)
                   ATCCTGCCATACCATTCACAACATTAAGTTCGAGATTTACCCCAAGTTTAAGAACTCACACCACTATGAATCTTGCCGAATTAAAGAATACGCC                                                                            <  SRR4302535.3586705/94‑1 (MQ=60)
                   ATCCTGCCATACCATTCACAACATTAAGTTCGAGATTTACCCCAAGTTTAAGAACTCACACCACTATGAATCTTGCCGAATTAAAGAATACGCC                                                                            >  SRR4302535.31419/1‑94 (MQ=60)
                   ATCCTGCCATACCATTCACAACATTAAGTTCGAGATTTACCCCAAGTTTAAGAACTCACACCACTATGAATCTTGCCGAATTAAAGAATACGCC                                                                            >  SRR4302535.1132119/1‑94 (MQ=60)
                    TCCTGCCATACCATTCACAACATTAAGTTCGAGATTTACCCCAAGTTTAAGAACTCACACCACTATGAATCTTGCCGAATTAAAGAATACGCCG                                                                           >  SRR4302535.3310552/1‑94 (MQ=60)
                    TCCTGCCATACCATTCACAACATTAAGTTCGAGATTTACCCCAAGTTTAAGAACTCACACCACTATGAATCTTGCCGAATTAAAGAATACGCCG                                                                           <  SRR4302535.279132/94‑1 (MQ=60)
                    TCCTGCCATACCATTCACAACATTAAGTTCGAGATTTACCCCAAGTTTAAGAACTCACACCACTATGAATCTTGCCGAATTAAAGAATACGCCG                                                                           <  SRR4302535.4348654/94‑1 (MQ=60)
                     CCTGCCATACCATTCACAACATTAAGTTCGAGATTTACCCCAAGTTTAAGAACTCACACCACTATGAATCTTGCCGAATTAAAGAATACGCCGG                                                                          >  SRR4302535.1566468/1‑94 (MQ=60)
                          CATACCATTCACAACATTAAGTTCGAGATTTACCCCAAGTTTAAGAACTCACACCACTATGAATCTTGCCGAATTAAAGAATACGCCGGTTTCT                                                                     <  SRR4302535.2729664/94‑1 (MQ=60)
                          CATACCATTCACAACATTAAGTTCGAGATTTACCCCAAGTTTAAGAACTCACACCACTATGAATCTTGCCGAATTAAAGAATACGCCGGTTTCT                                                                     >  SRR4302535.3598681/1‑94 (MQ=60)
                          CATACCATTCACAACATTAAGTTCGAGATTTACCCCAAGTTTAAGAACTCACACCACTATGAATCTTGCCGAATTAAAGAATACGCCGGTTTCT                                                                     <  SRR4302535.2135408/94‑1 (MQ=60)
                          CATACCATTCACAACATTAAGTTCGAGATTTACCCCAAGTTTAAGAACTCACACCACTATGAATCTTGCCGAATTAAAGAATACGCCGGTTTCT                                                                     >  SRR4302535.2084929/1‑94 (MQ=60)
                          CATACCATTCACAACATTAAGTTCGAGATTTACCCCAAGTTTAAGAACTCACACCACTATGAATCTTGCCGAATTAAAGAATACGCCGGTTTCT                                                                     >  SRR4302535.4492894/1‑94 (MQ=60)
                          CATACCATTCACAACATTAAGTTCGAGATTTACCCCAAGTTTAAGAACTCACACCACTATGAATCTTGCCGAATTAAAGAATACGCCGGTTTCT                                                                     >  SRR4302535.1550184/1‑94 (MQ=60)
                           ATACCATTCACAACATTAAGTTCGAGATTTACCCCAAGTTTAAGAACTCACACCACTATGAATCTTGCCGAATTAAAGAATACGCCGGTTTCTG                                                                    >  SRR4302535.3909399/1‑94 (MQ=60)
                           ATACCATTCACAACATTAAGTTCGAGATTTACCCCAAGTTTAAGAACTCACACCACTATGAATCTTGCCGAATTAAAGAATACGCCGGTTTCTG                                                                    >  SRR4302535.1763829/1‑94 (MQ=60)
                            TACCATTCACAACATTAAGTTCGAGATTTACCCCAAGTTTAAGAACTCACACCACTATGAATCTTGCCGAATTAAAGAATACGCCGGTTTCTGA                                                                   <  SRR4302535.3403168/94‑1 (MQ=60)
                             ACCATTCACAACATTAAGTTCGAGATTTACCCCAAGTTTAAGAACTCACACCACTATGAATCTTGCCGAATTAAAGAATACGCCGGTTTCTGAG                                                                  >  SRR4302535.494349/1‑94 (MQ=60)
                              CCATTCACAACATTAAGTTCGAGATTTACCCCAAGTTTAAGAACTCACACCACTATGAATCTTGCCGAATTAAAGAATACGCCGGTTTCTGAGC                                                                 >  SRR4302535.2173132/1‑94 (MQ=60)
                               CATTCACAACATTAAGTTCGAGATTTACCCCAAGTTTAAGAACTCACACCACTATGAATCTTGCCGAATTAAAGAATACGCCGGTTTCTGAGCT                                                                >  SRR4302535.101635/1‑94 (MQ=60)
                               CATTCACAACATTAAGTTCGAGATTTACCCCAAGTTTAAGAACTCACACCACTATGAATCTTGCCGAATTAAAGAATACGCCGGTTTCTGAGCT                                                                >  SRR4302535.435040/1‑94 (MQ=60)
                               CATTCACAACATTAAGTTCGAGATTTACCCCAAGTTTAAGAACTCACACCACTATGAATCTTGCCGAATTAAAGAATACGCCGGTTTCTGAGCT                                                                >  SRR4302535.3363586/1‑94 (MQ=60)
                                  TCACAACATTAAGTTCGAGATTTACCCCAAGTTTAAGAACTCACACCACTATGAATCTTGCCGAATTAAAGAATACGCCGGTTTCTGAGCTGAT                                                             <  SRR4302535.2631328/94‑1 (MQ=60)
                                  TCACAACATTAAGTTCGAGATTTACCCCAAGTTTAAGAACTCACACCACTATGAATCTTGCCGAATTAAAGAATACGCCGGTTTCTGAGCTGAT                                                             >  SRR4302535.1400394/1‑94 (MQ=60)
                                    ACAACATTAAGTTCGAGATTTACCCCAAGTTTAAGAACTCACACCACTATGAATCTTGCCGAATTAAAGAATACGCCGGTTTCTGAGCTGATCA                                                           <  SRR4302535.3736517/94‑1 (MQ=60)
                                    ACAACATTAAGTTCGAGATTTACCCCAAGTTTAAGAACTCACACCACTATGAATCTTGCCGAATTAAAGAATACGCCGGTTTCTGAGCTGATCA                                                           >  SRR4302535.1500788/1‑94 (MQ=60)
                                    ACAACATTAAGTTCGAGATTTACCCCAAGTTTAAGAACTCACACCACTATGAATCTTGCCGAATTAAAGAATACGCCGGTTTCTGAGCTGATCA                                                           >  SRR4302535.1203172/1‑94 (MQ=60)
                                    ACAACATTAAGTTCGAGATTTACCCCAAGTTTAAGAACTCACACCACTATGAATCTTGCCGAATTAAAGAATACGCCGGTTTCTGAGCTGATCA                                                           <  SRR4302535.3514127/94‑1 (MQ=60)
                                     CAACATTAAGTTCGAGATTTACCCCAAGTTTAAGAACTCACGCCACTATGAATCTTGCCGAATTAAAGAATACGCCGGTTTCTGAGCTGATCAC                                                          <  SRR4302535.4243953/94‑1 (MQ=60)
                                     CAACATTAAGTTCGAGATTTACCCCAAGTTTAAGAACTCACACCACTATGAATCTTGCCGAATTAAAGAATACGCCGGTTTCTGAGCTGATCAC                                                          <  SRR4302535.3670761/94‑1 (MQ=60)
                                     CAACATTAAGTTCGAGATTTACCCCAAGTTTAAGAACTCACACCACTATGAATCTTGCCGAATTAAAGAATACGCCGGTTTCTGAGCTGATCAC                                                          >  SRR4302535.2298525/1‑94 (MQ=60)
                                     CAACATTAAGTTCGAGATTTACCCCAAGTTTAAGAACTCACACCACTATGAATCTTGCCGAATTAAAGAATACGCCGGTTTCTGAGCTGATCAC                                                          <  SRR4302535.3416996/94‑1 (MQ=60)
                                      AACATTAAGTTCGAGATTTACCCCAAGTTTAAGAACTCACACCACTATGAATCTTGCCGAATTAAAGAATACGCCGGTTTCTGAGCTGATCACT                                                         <  SRR4302535.277165/94‑1 (MQ=60)
                                      AACATTAAGTTCGAGATTTACCCCAAGTTTAAGAACTCACACCACTATGAATCTTGCCGAATTAAAGAATACGCCGGTTTCTGAGCTGATCACT                                                         <  SRR4302535.2492541/94‑1 (MQ=60)
                                       ACATTAAGTTCGAGATTTACCCCAAGTTTAAGAACTCACACCACTATGAATCTTGCCGAATTAAAGAATACGCCGGTTTCTGAGCTGATCACTC                                                        >  SRR4302535.45927/1‑94 (MQ=60)
                                        CATTAAGTTCGAGATTTACCCCAAGTTTAAGAACTCACACCACTATGAATCTTGCCGAATTAAAGAATACGCCGGTTTCTGAGCTGATCACTCT                                                       >  SRR4302535.2456115/1‑94 (MQ=60)
                                         ATTAAGTTCGAGATTTACCCCAAGTTTAAGAACTCACACCACTATGAATCTTGCCGAATTAAAGAATACGCCGGTTTCTGAGCTGATCACTCTC                                                      >  SRR4302535.840566/1‑94 (MQ=60)
                                         ATTAAGTTCGAGATTTACCCCAAGTTTAAGAACTCACACCACTATGAATCTTGCCGAATTAAAGAATACGCCGGTTTCTGAGCTGATCACTCTC                                                      <  SRR4302535.3865447/94‑1 (MQ=60)
                                          TTAAGTTCGAGATTTACCCCAAGTTTAAGAACTCACACCACTATGAATCTTGCCGAATTAAAGAATACGCCGGTTTCTGAGCTGATCACTCTCG                                                     <  SRR4302535.4058971/94‑1 (MQ=60)
                                          TTAAGTTCGAGATTTACCCCAAGTTTAAGAACTCACACCACTATGAATCTTGCCGAATTAAAGAATACGCCGGTTTCTGAGCTGATCACTCTCG                                                     <  SRR4302535.1087426/94‑1 (MQ=60)
                                            AAGTTCGAGATTTACCCCAAGTTTAAGAACTCACACCACTATGAATCTTGCCGAATTAAAGAATACGCCGGTTTCTGAGCTGATCACTCTCGGC                                                   <  SRR4302535.2597411/94‑1 (MQ=60)
                                                 CGAGATTTACCCCAAGTTTAAGAACTCACACCACTATGAATCTTGCCGAATTAAAGAATACGCCGGTTTCTGAGCTGATCACTCTCGGCGAAAA                                              >  SRR4302535.2846578/1‑94 (MQ=60)
                                                  GAGATTTACCCCAAGTTTAAGAACTCACACCACTATGAATCTTGCCGAATTAAAGAATACGCCGGTTTCTGAGCTGATCACTCTCGGCGAAAAT                                             <  SRR4302535.2391338/94‑1 (MQ=60)
                                                  GAGATTTACCCCAAGTTTAAGAACTCACACCACTATGAATCTTGCCGAATTAAAGAATACGCCGGTTTCTGAGCTGATCACTCTCGGCGAAAAT                                             >  SRR4302535.360639/1‑94 (MQ=60)
                                                  GAGATTTACCCCAAGTTTAAGAACTCACACCACTATGAATCTTGCCGAATTAAAGAATACGCCGGTTTCTGAGCTGATCACTCTCGGCGAAAAT                                             >  SRR4302535.2462985/1‑94 (MQ=60)
                                                   AGATTTCCCCCAAGTTTAAGAACTCACACCACTATGAATCTTGCCGAATTAAAGAATACGCCGGTTTCTGAGCTGATCACTCTCGGCGAAAATA                                            <  SRR4302535.1354891/94‑1 (MQ=60)
                                                   AGATTTACCCCAAGTTTAAGAACTCACACCACTATGAATCTTGCCGAATTAAAGAATACGCCGGTTTCTGAGCTGATCACTCTCGGCGAAAATA                                            >  SRR4302535.3756187/1‑94 (MQ=60)
                                                   AGATTTACCCCAAGTTTAAGAACTCACACCACTATGAATCTTGCCGAATTAAAGAATACGCCGGTTTCTGAGCTGATCACTCTCGGCGAAAATA                                            >  SRR4302535.518447/1‑94 (MQ=60)
                                                    GATTTACCCCAAGCTTAAGAACTCACACCACTATGAATCTTGCCGAATTAAAGAATACGCCGGTTTCTGAGCTGATCACTCTCGGCGAAAATAT                                           <  SRR4302535.2696656/94‑1 (MQ=60)
                                                     ATTTACCCCAAGTTTAAGAACTCACACCACTATGAATCTTGCCGAATTAACGAATACGCCGGTTTCTGAGCTGATCACTCTCGGCGAAAATATG                                          <  SRR4302535.2528652/94‑1 (MQ=60)
                                                     ATTTACCCCAAGTTTAAGAACTCACACCACTATGAATCTTGCCGAATTAAAGAATACGCCGGTTTCTGAGCTGATCACTCTCGTCGAAAATATG                                          >  SRR4302535.464945/1‑94 (MQ=60)
                                                     ATTTACCCCAAGTTTAAGAACTCACACCACTATGAATCTTGCCGAATTAAAGAATACGCCGGTTTCTGAGCTGATCACTCTCGGCGAAAATATG                                          >  SRR4302535.3223812/1‑94 (MQ=60)
                                                     ATTTACCCCAAGTTTAAGAACTCACACCACTATGAATCTTGCCGAATTAAAGAATACGCCGGTTTCTGAGCTGATCACTCTCGGCGAAAATATG                                          <  SRR4302535.923508/94‑1 (MQ=60)
                                                      TTTACCCCAAGTTTAAGAACTCACACCACTATGAATCTTGCCGAATTAAAGAATACGCCGGTTTCTGAGCTGATCACTCTCGGCGAAAATATGG                                         >  SRR4302535.1304878/1‑94 (MQ=60)
                                                       TTACCCCAAGTTTAAGAACTCACACCACTATGAATCTTGCCGAATTAAAGAATACGCCGGTTTCTGAGCTGATCACTCTCGGCGAAAATATGGG                                        <  SRR4302535.4311486/94‑1 (MQ=60)
                                                       TTACCCCAAGTTTAAGAACTCACACCACTATGAATCTTGCCGAATTAAAGAATACGCCGGTTTCTGAGCTGATCACTCTCGGCGAAAATATGGG                                        <  SRR4302535.187717/94‑1 (MQ=60)
                                                       TTACCCCAAGTTTAAGAACTCACACCACTATGAATCTTGCCGAATTAAAGAATACGCCGGTTTCTGAGCTGATCACTCTCGGCGAAAATATGGG                                        <  SRR4302535.216858/94‑1 (MQ=60)
                                                       TTACCCCAAGTTTAAGAACTCACACCACTATGAATCTTGCCGAATTAAAGAATACGCCGGTTTCTGAGCTGATCACTCTCGGCGAAAATATGGG                                        <  SRR4302535.2242665/94‑1 (MQ=60)
                                                       TTACCCCAAGTTTAAGAACTCACACCACTATGAATCTTGCCGAATTAAAGAATACGCCGGTTTCTGAGCTGATCACTCTCGGCGAAAATATGGG                                        <  SRR4302535.4371408/94‑1 (MQ=60)
                                                        TACCCCAAGTTTAAGAACTCACACCACTATGAATCTTGCCGAATTAAAGAATACGCCGGTTTCTGAGCTGATCACTCTCGGCGAAAATATGGGG                                       <  SRR4302535.1211863/94‑1 (MQ=60)
                                                        TACCCCAAGTTTAAGAAATCACACCACTATGAATCTTGCCGAATTAAAGAATACGCCGGTTTCTGAGCTGATCACTCTCGGCGAAAATATGGGG                                       <  SRR4302535.1413484/94‑1 (MQ=60)
                                                         ACCCCAAGTTTAAGAACTCACACCACTATGAATCTTGCCGAATTAAAGAATACGCCGGTTTCTGAGCTGATCACTCTCGGCGAAAATATGGGGC                                      >  SRR4302535.2130801/1‑94 (MQ=60)
                                                          CCCCAAGTTTAAGAACTCACACCACTATGAATCTTGCCGAATTAAAGAATACGCCGGTTTCTGAGCTGATCACTCTCGGCGAAAATATGGGGCT                                     <  SRR4302535.933229/94‑1 (MQ=60)
                                                          CCCCAAGTTTAAGAACTCACACCACTATGAATCTTGCCGAATTAAAGAATACGCCGGTTTCTGAGCTGATCACTCTCGGCGAAAATATGGGGCT                                     >  SRR4302535.1531083/1‑94 (MQ=60)
                                                          CCCCAAGTTTAAGAACTCACACCACTATGAATCTTGCCGAATTAAAGAATACGCCGGTTTCTGAGCTGATCACTCTCGGCGAAAATATGGGGCT                                     >  SRR4302535.396301/1‑94 (MQ=60)
                                                          CCCCAAGTTTAAGAACTCACACCACTATGAATCTTGCCGAATTAAAGAATACGCCGGTTTCTGAGCTGATCACTCTCGCCGAAAATATGGGGCT                                     >  SRR4302535.879120/1‑94 (MQ=60)
                                                            CCAAGTTTAAGAACTCACACCACTATGAATCTTGCCGAATTAAAGAATACGCCGGTTTCTGAGCTGATCACTCTCGGCGAAAATATGGGGCTGG                                   <  SRR4302535.3302789/94‑1 (MQ=60)
                                                             CAAGTTTAAGAACTCACACCACTATGAATCTTGCCGAATTAAAGAATACGCCGGTTTCTGAGCTGATCACTCTCGGCGAAAATATGGGGCTGGA                                  >  SRR4302535.2086241/1‑94 (MQ=60)
                                                             CAAGTTTAAGAACTCACACCACTATGAATCTTGCCGAATTAAAGAATACGCCGGTTTCTGAGCTGATCACTCTCGGCGAAAATATGGGGCTGGA                                  <  SRR4302535.515316/94‑1 (MQ=60)
                                                              AAGTTTAAGAACTCACACCACTATGAATCTTGCCGAATTAAAGAATACGCCGGTTTCTGAGCTGATCACTCTCGGCGAAAATATGGGGCTGGAA                                 >  SRR4302535.1198490/1‑94 (MQ=60)
                                                              AAGTTTAAGAACTCACACCACTATGAATCTTGCCGAATTAAAGAATACGCCGGTTTCTGAGCTGATCACTCTCGGCGAAAATATGGGGCTGGAA                                 >  SRR4302535.1806106/1‑94 (MQ=60)
                                                               AGTTTAAGAACTCACACCACTATGAATCTTGCCGAATTAAAGAATACGCCGGTTTCTGAGCTGATCACTCTCGGCGAAAATATGGGGCTGGAAA                                <  SRR4302535.1957017/94‑1 (MQ=60)
                                                                 TTTAAGAACTCACACCACTATGAATCTTGCCGAATTAAAGAATACGCCGGTTTCTGAGCTGATCACTCTCGGCGAAAATATGGGGCTGGAAAAC                              >  SRR4302535.2727957/1‑94 (MQ=60)
                                                                  TTAAGAACTCACACCACTATGAATCTTGCCGAATTAAAGAATACGCCGGTTTCTGAGCTGATCACTCTCGGCGAAAATATGGGGCTGGAAAACC                             >  SRR4302535.4205767/1‑94 (MQ=60)
                                                                  TTAAGAACTCACACCACTATGAATCTTGCCGAATTAAAGAATACGCCGGTTTCTGAGCTGATCACTCTCGGCGAAAATATGGGGCTGGAAAACC                             <  SRR4302535.1800927/94‑1 (MQ=60)
                                                                   TAAGAACTCACACCACTATGAATCTTGCCGAATTAAAGAATACGCCGGTTTCTGAGCTGATCACTCTCGGCGAAAATATGGGGCTGGAAAACCT                            <  SRR4302535.1396046/94‑1 (MQ=60)
                                                                   TAAGAACTCACACCACTATGAATCTTGCCGAATTAAAGAATACGCCGGTTTCTGAGCTGATCACTCTCGGCGAAAATATGGGGCTGGAAAACCT                            <  SRR4302535.24430/94‑1 (MQ=60)
                                                                   TAAGAACTCACACCACTATGAATCTTGCCGAATTAAAGAATACGCCGGTTTCTGAGCTGATCACTCTCGGCGAAAATATGGGGCTGGAAAACCT                            <  SRR4302535.3982649/94‑1 (MQ=60)
                                                                   TAAGAACTCACACCACTATGAATCTTGCCGAATTAAAGAATACGCCGGTTTCTGAGCTGATCACTCTCGGCGAAAATATGGGGCTGGAAAACCT                            >  SRR4302535.1036930/1‑94 (MQ=60)
                                                                    AAGAACTCACACCACTATGAATCTTGCCGAATTAAAGAATACGCCGGTTTCTGAGCTGATCACTCTCGGCGAAAATATGGGGCTGGAAAACCTG                           >  SRR4302535.3068014/1‑94 (MQ=60)
                                                                    AAGAACTCACACCACTATGAATCTTGCCGAATTAAAGAATACGCCGGTTTCTGAGCTGATCACTCTCGGCGAAAATATGGGGCTGGAAAACCTG                           >  SRR4302535.1896590/1‑94 (MQ=60)
                                                                     AGAACTCACACCACTATGAATCTTGCCGAATTAAAGAATACGCCGGTTTCTGAGCTGATCACTCTCGGCGAAAATATGGGGCTGGAAAACCTGG                          <  SRR4302535.4482656/94‑1 (MQ=60)
                                                                      GAACTCACACCACTATGAATCTTGCCGAATTAAAGAATACGCCGGTTTCTGAGCTGATCACTCTCGGCGAAAATATGGGGCTGGAAAACCTGGC                         >  SRR4302535.2346609/1‑94 (MQ=60)
                                                                       AACTCACACCACTATGAATCTTGCCGAATTAAAGAATACGCCGGTTTCTGAGCTGATCACTCTCGGCGAAAATATGGGGCTGGAAAACCTGGCT                        >  SRR4302535.4613645/1‑94 (MQ=60)
                                                                       AACTCACACCACTATGAATCTTGCCGAATTAAAGAAGACGCCGGTTTCTGAGCTGATCACTCTCGGCGAAAATATGGGGCTGGAAAACCTGGCT                        <  SRR4302535.3604962/94‑1 (MQ=60)
                                                                        ACTCACACCACTATGAATCTTGCCGAATTAAAGAATACGCCGGTTTCTGAGCTGATCACTCTCGGCGAAAATATGGGGCTGGAAAACCTGGCTC                       <  SRR4302535.1051065/94‑1 (MQ=60)
                                                                        ACTCACACCACTATGAATCTTGCCGAATTAAAGAATACGCCGGTTTCTGAGCTGATCACTCTCGGCGAAAATATGGGGCTGGAAAACCTGGCTC                       <  SRR4302535.2128546/94‑1 (MQ=60)
                                                                        ACTCACACCACTATGAATCTTGCCGAATTAAAGAATACGCCGGTTTCTGAGCTGATCACTCTCGGCGAAAATATGGGGCTGGAAAACCTGGCTC                       <  SRR4302535.2243860/94‑1 (MQ=60)
                                                                         CTCACACCACTATGAATCTTGCCGAATTAAAGAATACGCCGGTTTCTGAGCTGATCACTCTCGGCGAAAATATGGGGCTGGAAAACCTGGCTCG                      >  SRR4302535.547554/1‑94 (MQ=60)
                                                                          TCACACCACTATGAATCTTGCCGAATTAAAGAATACGCCGGTTTCTGAGCTGATCACTCTCGGCGAAAATATGGGGCTGGAAAACCTGGCTCGT                     <  SRR4302535.1384837/94‑1 (MQ=60)
                                                                            ACACCACTATGAATCTTGCCGAATTAAAGAATACGCCGGTTTCTGAGCTGATCACTCTCGGCGAAAATATGGGGCTGGAAAACCTGGCTCGTAT                   <  SRR4302535.3232736/94‑1 (MQ=60)
                                                                             CACCACTATGAATCTTGCCGAATTAAAGAATACGCCGGTTTCTGAGCTGATCACTCTCGGCGAAAATATGGGGCTGGTAAACCTGGCTCGTATG                  >  SRR4302535.1222117/1‑94 (MQ=60)
                                                                             CACCACTATGAATCTTGCCGAATTAAAGAATACGCCGGTTTCTGAGCTGATCACTCTCGGCGAAAATATGGGGCTGGAAAACCTGGCTCGTATG                  <  SRR4302535.1427887/94‑1 (MQ=60)
                                                                             CACCACTATGAATCTTGCCGAATTAAAGAATACGCCGGTTTCTGAGCTGATCACTCTCGGCGAAAATATGGGGCTGGAAAACCTGGCTCGTATG                  >  SRR4302535.1099021/1‑94 (MQ=60)
                                                                             CACCACTATGAATCTTGCCGAATTAAAGAATACGCCGGTTTCTGAGCTGATCACTCTCGGCGAAAATATGGGGCTGGAAAACCTGGCTCGTATG                  >  SRR4302535.4003607/1‑94 (MQ=60)
                                                                              ACCACTATGAATCTTGCCGAATTAAAGAATACGCCGGTTTCTGAGCTGATCACTCTCGGCGAAAATATGGGGCTGGAAAACCTGGCTCGTATGC                 >  SRR4302535.4655798/1‑94 (MQ=60)
                                                                               CCACTATGAATCTTGCCGAATTAAAGAATACGCCGGTTTCTGAGCTGATCACTCTCGGCGAAAATATGGGGCTGGAAAACCTGGCTCGTATGCG                <  SRR4302535.1252209/94‑1 (MQ=60)
                                                                               CCACTATGAATCTTGCCGAATTAAAGAATACGCCGGTTTCTGAGCTGATCACTCTCGGCGAAAATATGGGGCTGGAAAACCTGGCTCGTATGAG                <  SRR4302535.3347249/94‑1 (MQ=60)
                                                                                 ACTATGAATCTTGCCGAATTAAAGAATACGCCGGTTTCTGAGCTGATCACTCTCGGCGAAAATATGGGGCTGGAAAACCTGGCTCGTATGCGTA              >  SRR4302535.979146/1‑94 (MQ=60)
                                                                                   TATGAATCTTGCCGAATTAAAGAATACGCCGGTTTCTGAGCTGATCACTCTCGGCGAAAATATGGGGCTGGAAAACCTGGCTCGTATGCGTAAG            <  SRR4302535.1804623/94‑1 (MQ=60)
                                                                                   TATGAATCTTGCCGAATTAAAGAATACGCCGGTTTCTGAGCTGATCACTCTCGGCGAAAATATGGGGCTGGAAAACCTGGCTCGTATGCGTAAG            <  SRR4302535.4172778/94‑1 (MQ=60)
                                                                                   TATGAATCTTGCCGAATTAAAGAATACGCCGGTTTCTGAGCTGATCACTCTCGGCGAAAATATGGGGCTGGAAAACCTGGCTCGTATGCGTAAG            <  SRR4302535.2772811/94‑1 (MQ=60)
                                                                                   TATGAATCTTGCCGAATTAAAGAATACGCCGGTTTCTGAGCTGATCACTCTCGGCGAAAATATGGGGCTGGAAAACCTGGCTCGTATGCGTAAG            <  SRR4302535.3245509/94‑1 (MQ=60)
                                                                                    ATGAATCTTGCCGAATTAAAGAATACGCCGGTTTCTGAGCTGATCACTCTCGGCGAAAATATGGGGCTGGAAAACCTGGCTCGTATGCGTAAGC           >  SRR4302535.2033208/1‑94 (MQ=60)
                                                                                     TGAATCTTGCCGAATTAAAGAATACGCCGGTTTCTGAGCTGATCACTCTCGGCGAAAATATGGGGCTGGAAAACCTGGCTCGTATGCGTAAGCA          >  SRR4302535.1416972/1‑94 (MQ=60)
                                                                                       AATCTTGCCGAATTAAAGAATACGCCGGTTTCTGAGCTGATCACTCTCGGCGAAAATATGGGGCTGGAAAACCTGGCTCGTATGCGTAAGCAGG        <  SRR4302535.1698494/94‑1 (MQ=60)
                                                                                       AATCTTGCCGAATTAAAGAATACGCCGGTTTCTGAGCTGATCACTCTCGGCGAAAATATGGGGCTGGAAAACCTGGCTCGTATGCGTAAGCAGG        >  SRR4302535.4443311/1‑94 (MQ=60)
                                                                                       AATCTTGCCGAATTAAAGAATACGCCGGTTTCTGAGCTGATCACTCTCGGCGAAAATATGGGGCTGGAAAACCTGGCTCGTATGCGTAAGCAGG        <  SRR4302535.1201645/94‑1 (MQ=60)
                                                                                         TCTTGCCGAATTAAAGAATACGCCGGTTTCTGAGCTGATCACTCTCGGCGAAAATATGGGGCTGGAAAACCTGGCTCGTATGCGTAAGCAGGAC      <  SRR4302535.2256174/94‑1 (MQ=60)
                                                                                          CTTGCCGAATTAAAGAATACGCCGGTTTCTGAGCTGATCACTCTCGGCGAAAATATGGGGCTGGAAAACCTGGCTCGTATGCGTAAGCAGGACA     >  SRR4302535.1486196/1‑94 (MQ=60)
                                                                                          CTTGCCGAATTAAAGAATACGCCGGTTTCTGAGCTGATCACTCTCGGCGAAAATATGGGGCTGGAAAACCTGGCTCGTATGCGTAAGCAGGACA     <  SRR4302535.3722493/94‑1 (MQ=60)
                                                                                          CTTGCCGAATTAAAGAATACGCCGGTTTCTGAGCTGATCACTCTCGGCGAAAATATGGGGCTGGAAAACCTGGCTCGGATGCGTAAGCAGGACA     >  SRR4302535.3541074/1‑94 (MQ=60)
                                                                                           TTGCCGAATTAAAGAATACGCCGGTTTCTGAGCTGATCACTCTCGGCGAAAATATGGGGCTGGAAAACCTGGCTCGTATGCGTAAGCAGGACAT    >  SRR4302535.3366821/1‑94 (MQ=60)
                                                                                           TTGCCGAATTAAAGAATACGCCGGTTTCTGAGCTGATCACTCTCGGCGAAAATATGGGGCTGGAAAACCTGGCTCGTATGCGTAAGCAGGACAT    <  SRR4302535.1128600/94‑1 (MQ=60)
                                                                                            TGCCGAATTAAAGAATACGCCGGTTTCTGAGCTGATCACTCTCGGCGAAAATATGGGGCTGGAAAACCTGGCTCGTATGCGTAAGCAGGACATT   <  SRR4302535.603071/94‑1 (MQ=60)
                                                                                             GCCGAATTAAAGAATACGCCGGTTTCTGAGCTGATCACTCTCGGCGAAAATATGGGGCTGGAAAACCTGGCTCGTATGCGTAAGCAGGACATTA  <  SRR4302535.3694207/94‑1 (MQ=60)
                                                                                             |                                                                                             
CCGTAAACAGGCATGGATGATCCTGCCATACCATTCACAACATTAAGTTCGAGATTTACCCCAAGTTTAAGAACTCACACCACTATGAATCTTACCGAATTAAAGAATACGCCGGTTTCTGAGCTGATCACTCTCGGCGAAAATATGGGGCTGGAAAACCTGGCTCGTATGCGTAAGCAGGACATTA  >  NC_000913/3963948‑3964134

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑