Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I2 R1	1873	183.1	9376148	98.2%	9207377	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,214,346	A→G	E430E (GAA→GAG)	aceB →	malate synthase A

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,214,346	0	A	G	100.0%	771.6 / NA	243	E430E (GAA→GAG)	aceB	malate synthase A
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (111/132); total (111/132)

Only 100 of 254 total aligned reads displayed.

TACTGCCGATCAGCTGCTGGCACCTTGTGATGGTGAACGCACCGAAGAAGGTATGCGCGCCAACATTCGCGTGGCTGTGCAGTACATCGAAGCGTGGATCTCTGGCAACGGCTGTGTGCCGATTTATGGCCTGATGGAAGATGCGGCGACGGCTGAAATTTCCCGTACCTCGATCTGGC  >  NC_000913/4214256‑4214434

 aCTGCCGATCAGCTGCTGGCACCTTGTGATGGTGAACGCACCGAAGAAGGTATGCGCGCCAACATTCGCGTGGCTGTGCAGTACATCGAGGCg                                                                                       <  2:3978474/93‑1 (MQ=255)

 aCTGCCGATCAGCTGCTGGCACCTTGTGATGGTGAACGCACCGAAGAAGGTATGCGCGCCAACATTCGCGTGGCTGTGCAGTACATCGAGGCg                                                                                       >  1:3401203/1‑93 (MQ=255)

    gCCGATCAGCTGCTGGCACCTTGTGATGGTGAACGCACCGAAGAAGGTATGCGCGCCAACATTCGCGTGGCTGTGCAGTACATCGAGGCGTgg                                                                                    <  2:14560/93‑1 (MQ=255)

    gCCGATCAGCTGCTGGCACCTTGTGATGGTGAACGCACCGAAGAAGGTATGCGCGCCAACATTCGCGTGGCTGTGCAGTACATCGAGGCGTgg                                                                                    >  2:2643112/1‑93 (MQ=255)

    gCCGATCAGCTGCTGGCACCTTGTGATGGTGAACGCACCGAAGAAGGTATGCGCGCCAACATTCGCGTGGCTGTGCAGTACATCGAGGCGTgg                                                                                    <  1:2293451/93‑1 (MQ=255)

      cGATCAGCTGCTGGCACCTTGTGATGGTGAACGCACCGAAGAAGGTATGCGCGCCAACATTCGCGTGGCTGTGCAGTACATCGAGGCGTGGAt                                                                                  <  2:1300356/93‑1 (MQ=255)

      cGATCAGCTGCTGGCACCTTGTGATGGTGAACGCACCGAAGAAGGTATGCGCGCCAACATTCGCGTGGCTGTGCAGTACATCGAGGCGTGGAt                                                                                  >  1:2564607/1‑93 (MQ=255)

         tCAGCTGCTGGCACCTTGTGATGGTGAACGCACCGAAGAAGGTATGCGCGCCAACATTCGCGTGGCTGTGCAGTACATCGAGGCGTGGAtctc                                                                               >  2:2379697/1‑93 (MQ=255)

         tCAGCTGCTGGCACCTTGTGATGGTGAACGCACCGAAGAAGGTATGCGCGCCAACATTCGCGTGGCTGTGCAGTACATCGAGGCGTGGAtctc                                                                               >  2:2409267/1‑93 (MQ=255)

          cAGCTGCTGGCACCTTGTGATGGTGAACGCACCGAAGAAGGTATGCGCGCCAACATTCGCGTGGCTGTGCAGTACATCGAGGCGTGGATctct                                                                              <  1:146097/93‑1 (MQ=255)

          cAGCTGCTGGCACCTTGTGATGGTGAACGCACCGAAGAAGGTATGCGCGCCAACATTCGCGTGGCTGTGCAGTACATCGAGGCGTGGATctct                                                                              <  1:1563533/93‑1 (MQ=255)

            gctgctGGCACCTTGTGATGGTGAACGCACCGAAGAAGGTATGCGCGCCAACATTCGCGTGGCTGTGCAGTACATCGAGGCGTGGATCTCTgg                                                                            >  2:3607011/1‑93 (MQ=255)

            gctgctGGCACCTTGTGATGGTGAACGCACCGAAGAAGGTATGCGCGCCAACATTCGCGTGGCTGTGCAGTACATCGAGGCGTGGATCTCTgg                                                                            >  1:113587/1‑93 (MQ=255)

              tgctgGCACCTTGTGATGGTGAACGCACCGAAGAAGGTATGCGCGCCAACATTCGCGTGGCTGTGCAGTACATCGAGGCGTGGATCTCTGGCa                                                                          >  2:2279773/1‑93 (MQ=255)

              tgctgGCACCTTGTGATGGTGAACGCACCGAAGAAGGTATGCGCGCCAACATTCGCGTGGCTGTGCAGTACATCGAGGCGTGGATCTCTGGCa                                                                          <  1:2795700/93‑1 (MQ=255)

              tgctgGCACCTTGTGATGGTGAACGCACCGAAGAAGGTATGCGCGCCAACATTCGCGTGGCTGTGCAGTACATCGAGGCGTGGATCTCTGGCa                                                                          >  1:1043031/1‑93 (MQ=255)

              tgctgGCACCTTGTGATGGTGAACGCACCGAAGAAGGTATGCGCGCCAACATTCGCGTGGCTGTGCAGTACATCGAGGCGGGGATCTCTGGCa                                                                          >  1:3289646/1‑93 (MQ=255)

               gctgGCACCTTGTGATGGTGAACGCACCGAAGAAGGTATGCGCGCCAACATTCGCGTGGCTGTGCAGTACATCGAGGCGTGGATCTCTGGCaa                                                                         <  2:1139205/93‑1 (MQ=255)

               gctgGCACCTTGTGATGGTGAACGCACCGAAGAAGGTATGCGCGCCAACATTCGCGTGGCTGTGCAGTACATCGAGGCGTGGATCTCTGGCaa                                                                         <  2:3530489/93‑1 (MQ=255)

                ctgGCACCTTGTGATGGTGAACGCACCGAAGAAGGTATGCGCGCCAACATTCGCGTGGCTGTGCAGTACATCGAGGCGTGGATCTCTGGCAAc                                                                        <  1:4327540/93‑1 (MQ=255)

                  ggCACCTTGTGATGGTGAACGCACCGAAGAAGGTATGCGCGCCAACATTCGCGTGGCTGTGCAGTACATCGAGGCGTGGATCTCTGGCAACgg                                                                      >  1:1185412/1‑93 (MQ=255)

                   gCACCTTGTGATGGTGAACGCACCGAAGAAGGTATGCGCGCCAACATTCGCGTGGCTGTGCAGTACATCGAGGCGTGGATCTCTGGCAACGGc                                                                     <  2:4370486/93‑1 (MQ=255)

                     aCCTTGTGATGGTGAACGCACCGAAGAAGGTATGCGCGCCAACATTCGCGTGGCTGTGCAGTACATCGAGGCGTGGATCTCTGGCAACGGCtg                                                                   >  2:3676676/1‑93 (MQ=255)

                     aCCTTGTGATGGTGAACGCACCGAAGAAGGTATGCGCGCCAACATTCGCGTGGCTGTGCAGTACATCGAGGCGTGGATCTCTGGCAACGGCtg                                                                   >  1:1269902/1‑93 (MQ=255)

                      ccTTGTGATGGTGAACGCACCGAAGAAGGTATGCGCGCCAACATTCGCGTGGCTGTGCAGTACATCGAGGCGTGGATCTCTGGCAACGGCtgt                                                                  >  1:2433712/1‑93 (MQ=255)

                      ccTTGTGATGGTGAACGCACCGAAGAAGGTATGCGCGCCAACATTCGCGTGGCTGTGCAGTACATCGAGGCGTGGATCTCTGGCAACGGCtgt                                                                  >  1:2157936/1‑93 (MQ=255)

                       cTTGTGATGGTGAACGCACCGAAGAAGGTATGCGCGCCAACATTCGCGTGGCTGTGCAGTACATCGAGGCGTGGATCTCTGGCAACGGCtgtg                                                                 >  2:2743474/1‑93 (MQ=255)

                       cTTGTGATGGTGAACGCACCGAAGAAGGTATGCGCGCCAACATTCGCGTGGCTGTGCAGTACATCGAGGCGTGGATCTCTGGCAACGGCtgtg                                                                 >  1:3453209/1‑93 (MQ=255)

                        ttGTGATGGTGAACGCACCGAAGAAGGTATGCGCGCCAACATTCGCGTGGCTGTGCAGTACATCGAGGCGTGGATCTCTGGCAACGGCtgagt                                                                >  2:1755398/1‑93 (MQ=255)

                         tgtgATGGTGAACGCACCGAAGAAGGTATGCGCGCCAACATTCGCGTGGCTGTGCAGTACATCGAGGCGTGGATCTCTGGCAACGGCtgtgtg                                                               >  1:1313441/1‑93 (MQ=255)

                             aTGGTGAACGCACCGAAGAAGGTATGCGCGCCAACATTCGCGTGGCTGTGCAGTACATCGAGGCGTGGATCTCTGGCAACGGCTGTGTGCCGa                                                           <  1:2096677/93‑1 (MQ=255)

                              tGGTGAACGCACCGAAGAAGGTATGCGCGCCAACATTCGCGTGGCTGTGCAGTACATCGAGGCGTGGATCTCTGGCAACGGCTGTGTGCCGAt                                                          >  2:3317176/1‑93 (MQ=255)

                                gTGAACGCACCGAAGAAGGTATGCGCGCCAACATTCGCGTGGCTGTGCAGTACATCGAGGCGTGGATCTCTGGCAACGGCTGTGTGCCGAttt                                                        <  1:235787/93‑1 (MQ=255)

                                 tGAACGCACCGAAGAAGGTATGCGCGCCAACATTCGCGTGGCTGTGCAGTACATCGAGGCGTGGATCTCTGGCAACGGCTGTGTGCCGATTTa                                                       <  2:2615932/93‑1 (MQ=255)

                                  gAACGCACCGAAGAAGGTATGCGCGCCAACATTCGCGTGGCTGTGCAGTACATCGAGGCGTGGATCTCTGGCAACGGCTGTGTGCCGATTTAt                                                      <  1:3577598/93‑1 (MQ=255)

                                   aaCGCACCGAAGAAGGTATGCCCTCCAACATTCGCGGGGCTGTGCAGTACATCGAGGCGTGGATCTCTGGCAACGGCTGTGTGCCGATTTATg                                                     <  2:501029/93‑1 (MQ=255)

                                      gCACCGAAGAAGGTATGCGCGCCAACATTCGCGTGGCTGTGCAGTACATCGAGGCGTGGATCTCTGGCAACGGCTGTGTGCCGATTTATGGcc                                                  <  1:2974115/93‑1 (MQ=255)

                                       cACCGAAGAAGGTATGCGCGCCAACATTCGCGTGGCTGTGCAGTACATCGAGGCGTGGATCTCTGGCAACGGCTGTGTGCCGATTTATGGCCt                                                 >  2:1474811/1‑93 (MQ=255)

                                         ccGAAGAAGGTATGCGCGCCAACATTCGCGTGGCTGTGCAGTACATCGAGGCGTGGATCTCTGGCAACGGCTGTGTGCCGATTTATGGCCTGa                                               >  2:4307341/1‑93 (MQ=255)

                                          cGAAGAAGGTATGCGCGCCAACATTCGCGTGGCTGTGCAGTACATCGAGGCGTGGATCTCTGGCAACGGCTGTGTGCCGATTTATGGCCTGAt                                              <  1:2693525/93‑1 (MQ=255)

                                           gaagaaGGTATGCGCGCCAACATTCGCGTGGCTGTGCAGTACATCGAGGCGTGGATCTCTGGCAACGGCTGTGTGCCGATTTATGGCCTGATg                                             <  1:3824110/93‑1 (MQ=255)

                                           gaagaaGGTATGCGCGCCAACATTCGCGTGGCTGTGCAGTACATCGAGGCGTGGATCTCTGGCAACGGCTGTGTGCCGATTTATGGCCTGATg                                             >  2:1783866/1‑93 (MQ=255)

                                            aagaagGTATGCGCGCCAACATTCGCGTGGCTGTGCAGTACATCGAGGCGTGGATCTCTGGCAACGGCTGTGTGCCGATTTATGGCCTGATgg                                            <  1:3741318/93‑1 (MQ=255)

                                            aagaagGTATGCGCGCCAACATTCGCGTGGCTGTGCAGTACATCGAGGCGTGGATCTCTGGCAACGGCTGTGTGCCGATTTATGGCCTGATgg                                            >  1:3691743/1‑93 (MQ=255)

                                            aagaagGTATGCGCGCCAACATTCGCGTGGCTGTGCAGTACATCGAGGCGTGGATCTCTGGCAACGGCTGTGTGCCGATTTATGGCCTGATgg                                            >  2:1048545/1‑93 (MQ=255)

                                            aagaagGTATGCGCGCCAACATTCGCGTGGCTGTGCAGTACATCGAGGCGTGGATCTCTGGCAACGGCTGTGTGCCGATTTATGGCCTGATgg                                            <  1:2406369/93‑1 (MQ=255)

                                             agaagGTATGCGCGCCAACATTCGCGTGGCTGTGCAGTACATCGAGGCGTGGATCTCTGGCAACGGCTGTGTGCCGATTTATGGCCTGATGGa                                           >  1:3092337/1‑93 (MQ=255)

                                              gaagGTATGCGCGCCAACATTCGCGTGGCTGTGCAGTACATCGAGGCGTGGATCTCTGGCAACGGCTGTGTGCCGATTTATGGCCTGATGGaa                                          >  2:3806123/1‑93 (MQ=255)

                                              gaagGTATGCGCGCCAACATTCGCGTGGCTGTGCAGTACATCGAGGCGTGGATCTCTGGCAACGGCTGTGTGCCGATTTATGGCCTGATGGaa                                          >  1:760741/1‑93 (MQ=255)

                                                agGTATGCGCGCCAACATTCGCGTGGCTGTGCAGTACATCGAGGCGTGGATCTCTGGCAACGGCTGTGTGCCGATTTATGGCCTGATGGAAGa                                        >  2:2136986/1‑93 (MQ=255)

                                                agGTATGCGCGCCAACATTCGCGTGGCTGTGCAGTACATCGAGGCGTGGATCTCTGGCAACGGCTGTGTGCCGATTTATGGCCTGATGGAAGa                                        <  2:2855924/93‑1 (MQ=255)

                                                agGTATGCGCGCCAACATTCGCGTGGCTGTGCAGTACATCGAGGCGTGGATCTCTGGCAACGGCTGTGTGCCGATTTATGGCCTGATGGAAGa                                        >  2:1212488/1‑93 (MQ=255)

                                                 ggTATGCGCGCCAACATTCGCGTGGCTGTGCAGTACATCGAGGCGTGGATCTCTGGCAACGGCTGTGTGCCGATTTATGGCCTGATGGAAGAt                                       >  1:574250/1‑93 (MQ=255)

                                                 ggTATGCGCGCCAACATTCGCGTGGCTGTGCAGTACATCGAGGCGTGGATCTCTGGCAACGGCTGTGTGCCGATTTATGGCCTGATGGAAGAt                                       <  1:4071572/93‑1 (MQ=255)

                                                 ggTATGCGCGCCAACATTCGCGTGGCTGTGCAGTACATCGAGGCGTGGATCTCTGGCAACGGCTGTGTGCCGATTTATGGCCTGATGGAAGAt                                       >  2:4393080/1‑93 (MQ=255)

                                                   tATGCGCGCCAACATTCGCGTGGCTGTGCAGTACATCGGGGCGTGGATCTCTGGCAACGGCTGTGTGCCGATTTATGGCCTGATGGAAGATgc                                     >  2:1658004/1‑93 (MQ=255)

                                                   tATGCGCGCCAACATTCGCGTGGCTGTGCAGTACATCGAGGCGTGGATCTCTGGCAACGGCTGTGTGCCGATTTATGGCCTGATGGAAGATgc                                     >  2:4163654/1‑93 (MQ=255)

                                                   tATGCGCGCCAACATTCGCGTGGCTGTGCAGTACATCGAGGCGTGGATCTCTGGCAACGGCTGTGTGCCGATTTATGGCCTGATGGAAGATgc                                     >  1:271015/1‑93 (MQ=255)

                                                   tATGCGCGCCAACATTCGCGTGGCTGTGCAGTACATCGAGGCGTGGATCTCTGGCAACGGCTGTGTGCCGATTTATGGCCTGATGGAAGATgc                                     >  2:3272172/1‑93 (MQ=255)

                                                   tATGCGCGCCAACATTCGCGTGGCTGTGCAGTACATCGAGGCGTGGATCTCTGGCAACGGCTGTGTGCCGATTTATGGCCTGATGGAAGATgc                                     >  2:759505/1‑93 (MQ=255)

                                                   tATGCGCGCCAACATGCGCGTGGCTGTGCAGTACATCGAGGCGTGGATCTCTGGCAACGGCTGTGTGCCGATTTATGGCCTGATGGAAGATgc                                     <  2:2300692/93‑1 (MQ=255)

                                                    aTGCGCGCCAACATTCGCGTGGCTGTGCAGTACATCGAGGCGTGGATCTCTGGCAACGGCTGTGTGCCGATTTATGGCCTGATGGAAGATgcg                                    <  1:170451/93‑1 (MQ=255)

                                                      gcgcgcCAACATTCGCGTGGCTGTGCAGTACATCGAGGCGTGGATCTCTGGCAACGGCTGTGTGCCGATTTATGGCCTGATGGAAGATgcggc                                  <  2:2125208/93‑1 (MQ=255)

                                                      gcgcgcCAACATTCGCGTGGCTGTGCAGTACATCGAGGCGTGGATCTCTGGCAACGGCTGTGTGCCGATTTATGGCCTGATGGAAGATgcggc                                  <  1:3937378/93‑1 (MQ=255)

                                                        gcgcCAACATTCGCGTGGCTGTGCAGTACATCGAGGCGTGGATCTCTGGCAACGGCTGTGTGCCGATTTATGGCCTGATGGAAGATGCGGCGa                                >  1:854634/1‑93 (MQ=255)

                                                        gcgcCAACATTCGCGTGGCTGTGCAGTACATCGAGGCGTGGATCTCTGGCAACGGCTGTGTGCCGATTTATGGCCTGATGGAAGATGCGGCGa                                >  2:3011479/1‑93 (MQ=255)

                                                         cgcCAACATTCGCGTGGCTGTGCAGTACATCGAGGCGTGGATCTCTGGCAACGGCTGTGTGCCGATTTATGGCCTGATGGAAGATGCGGCGAc                               <  2:4101550/93‑1 (MQ=255)

                                                         cgcCAACATGCGCGTGGCTGTGCAGTACATCGAGGCGTGGATCTCTGGCAACGGCTGTGTGCCGATTTATGGCCTGATGGAAGATGCGGCGAc                               <  2:3443474/93‑1 (MQ=255)

                                                           ccAACATTCGCGTGGCTGTGCAGTACATCGAGGCGTGGATCTCTGGCAACGGCTGTGTGCCGATTTATGGCCTGATGGAAGATGAGGCGACgg                             <  2:4558259/93‑1 (MQ=255)

                                                            cAACATTCGCGTGGCTGTGCAGTACATCGAGGCGTGGATCTCTGGCAACGGCTGTGTGCCGATTTATGGCCTGATGGAAGATGCGGCGACGGc                            <  1:4592055/93‑1 (MQ=255)

                                                            cAACATTCGCGTGGCTGTGCAGTACATCGAGGCGTGGATCTCTGGCAACGGCTGTGTGCCGATTTATGGCCTGATGGAAGATGCGGCGACGGc                            <  2:2574345/93‑1 (MQ=255)

                                                             aaCATTCGCGTGGCTGTGCAGTACATCGAGGCGTGGATCTCTGGCAACGGCTGTGTGCCGATTTATGGCCTGATGGAAGATGCGGCGACGGCt                           >  1:3380630/1‑93 (MQ=255)

                                                              aCATTCGCGTGGCTGTGCAGTACATCGAGGCGTGGATCTCTGGCAACGGCTGTGTGCCGATTTATGGCCTGATGGAAGATGCGGCGACGGCTg                          >  1:4053358/1‑93 (MQ=255)

                                                                 ttCGCGTGGCTGTGCAGTACATCGAGGCGTGGATCTCTGGCAACGGCTGTGTGCCGATTTATGGCCTGATGGAAGATGCGGCGACGGCTGaaa                       >  1:2150794/1‑93 (MQ=255)

                                                                 ttCGCGTGGCTGTGCAGTACATCGAGGCGTGGATCTCTGGCAACGGCTGTGTGCCGATTTATGGCCTGATGGAAGATGCGGCGACGGCTGaaa                       >  1:2851681/1‑93 (MQ=255)

                                                                    gcgTGGCTGTGCAGTACATCGAGGCGTGGATCTCTGGCAACGGCTGTGTGCCGATTTATGGCCTGATGGAAGATGCGGCGACGGCTGAAAttt                    >  1:192706/1‑93 (MQ=255)

                                                                     cgTGGCTGTGCAGTACATCGAGGCGTGGATCTCTGGCAACGGCTGTGTGCCGATTTATGGCCTGATGGAAGATGCGGCGACGGCTGAAATTTc                   <  1:2114756/93‑1 (MQ=255)

                                                                      gTGGCTGTGCAGTACATCGAGGCGTGGATCTCTGGCAACGGCTGTGTGCCGATTTATGGCCTGATGGAAGATGCGGCGACGGCTGAAATTTcc                  <  1:2556242/93‑1 (MQ=255)

                                                                      gTGGCTGTGCAGTACATCGAGGCGTGGATCTCTGGCAACGGCTGTGTGCCGATTTATGGCCTGATGGAAGATGCGGCGACGGCTGAAATTTcc                  <  2:2466388/93‑1 (MQ=255)

                                                                      gTGGCTGTGCAGTACATCGAGGCGTGGATCTCTGGCAACGGCTGTGTGCCGATTTATGGCCTGATGGAAGATGCGGCGACGGCTGAAATTTcc                  <  2:3135823/93‑1 (MQ=255)

                                                                      gTGGCTGTGCAGTACATCGAGGCGTGGATCTCTGGCAACGGCTGTGTGCCGATTTATGGCCTGATGGAAGATGCGGCGACGGCTGAAATTTcc                  <  1:178008/93‑1 (MQ=255)

                                                                       tGGCTGTGCAGTACATCGAGGCGTGGATCTCTGGCAACGGCTGTGTGCCGATTTATGGCCTGATGGAAGATGCGGCGACGGCTGAAATTTccc                 <  2:2097081/93‑1 (MQ=255)

                                                                        ggCTGTGCAGTACATCGAGGCGTGGATCTCTGGCAACGGCTGTGTGCCGATTTATGGCCTGATGGAAGATGCGGCGACGGCTGAAATTTCCCg                >  1:1016071/1‑93 (MQ=255)

                                                                        ggCTGCGCAGTACATCGAGACGTGAATCTCTGGCAACGGCTGTGTGCCGATTTATGGCCTGATGGAAGATGCGGCGACGGCTGAAATTTCCCg                <  1:4664899/93‑1 (MQ=255)

                                                                            gtgCAGTACATCGAGGCGTGGATCTCTGGCAACGGCTGTGTGCCGATTTATGGCCTGATGGAAGATGCGGCGACGGCTGAAATTTCCCGTAcc            <  2:2887640/93‑1 (MQ=255)

                                                                              gCAGTACATCGAGGCGTGGATCTCTGGCAACGGCTGTGTGCCGATTTATGGCCTGATGGAAGATGCGGCGACGGCTGAAATTTCCCGTACCTc          <  1:722103/93‑1 (MQ=255)

                                                                                 gTACATCGAGGCGTGGATCTCTGGCAACGGCTGTGTGCCGATTTATGGCCTGATGGAAGATGCGGCGACGGCTGAAATTTCCCGTACCTCGAt       >  1:1515024/1‑93 (MQ=255)

                                                                                 gTACATCGAGGCGTGGATCTCTGGCAACGGCTGTGTGCCGATTTATGGCCTGATGGAAGATGCGGCGACGGCTGAAATTTCCCCTACCTCGAt       >  2:107196/1‑93 (MQ=255)

                                                                                  tACATCGAGGCGTGGATCTCTGGCAACGGCTGTGTGCCGATTTATGGCCTGATGGAAGATGCGGCGACGGCTGAAATTTCCCGTACCTCGATc      >  2:1865152/1‑93 (MQ=255)

                                                                                  tACATCGAGGCGTGGATCTCTGGCAACGGCTGTGTGCCGATTTATGGCCTGATGGAAGATGCGGCGACGGCTGAAATTTCCCGTACCTCGATc      <  1:1623065/93‑1 (MQ=255)

                                                                                  tACATCGAGGCGTGGATCTCTGGCAACGGCTGTGTGCCGATTTATGGCCTGATGGAAGATGCGGCGACGGCTGAAATTTCCCGTACCTCGATc      >  2:3225710/1‑93 (MQ=255)

                                                                                   tcATCGAGGCGTGGATCTCTGGCAACGGCTGTGTGCCGATTTATGGCCTGATGGAAGATGCGGCGACGGCTGAAATTTCCCGTACCTCGATCt     <  1:4460791/92‑1 (MQ=255)

                                                                                   tcATCGAGGCGTGGATCTCTGGCAACGGCTGTGTGCCGATTCATGGCCTGCTGGAAGCTGCGGCGACGGCTGAAATTTCCCGTACCTCGATCt     <  2:676061/92‑1 (MQ=255)

                                                                                   aCATCGAGGCGTGGATCTCTGGCAACGGCTGTGTGCCGATTTATGGCCTGATGGAAGATGCGGCGACGGCTGAAATTTCCCGTACCTCGATCt     <  2:2012574/93‑1 (MQ=255)

                                                                                    cATCGAGGCGTGGATCTCTGGCAACGGCTGTGTGCCGATTTATGGCCTGATGGAAGATGCGGCGACGGCTGAAATTTCCCGTACCTCGATCTg    >  1:88563/1‑93 (MQ=255)

                                                                                    cATCGAGGCGTGGATCTCTGGCAACGGCTGTGTGCCGATTTATGGCCTGATGGAAGATGCGGCGACGGCTGAAATTTCCCGTACCTCGATCTg    <  2:4591508/93‑1 (MQ=255)

                                                                                      tCGAGGCGTGGATCTCTGGCAACGGCTGTGTGCCGATTTATGGCCTGATGGAAGATGCGGCGACGGCTGAAATTTCCCGTACCTCGATCTGGc  <  2:559497/93‑1 (MQ=255)

                                                                                      tCGAGGCGTGGATCTCTGGCAACGGCTGTGTGCCGATTTATGGCCTGATGGAAGATGCGGCGACGGCTGAAATTTCCCGTACCTCGATCTGGc  <  1:4303303/93‑1 (MQ=255)

                                                                                      tCGAGGCGTGGATCTCTGGCAACGGCTGTGTGCCGATTTATGGCCTGATGGAAGATGCGGCGACGGCTGAAATTTCCCGTACCTCGATCTGGc  <  1:3867501/93‑1 (MQ=255)

                                                                                      tCGAGGCGTGGATCTCTGGCAACGGCTGTGTGCCGATTTATGGCCTGATGGAAGATGCGGCGACGGCTGAAATTTCCCGTACCTCGATCTGGc  >  2:961545/1‑93 (MQ=255)

TACTGCCGATCAGCTGCTGGCACCTTGTGATGGTGAACGCACCGAAGAAGGTATGCGCGCCAACATTCGCGTGGCTGTGCAGTACATCGAAGCGTGGATCTCTGGCAACGGCTGTGTGCCGATTTATGGCCTGATGGAAGATGCGGCGACGGCTGAAATTTCCCGTACCTCGATCTGGC  >  NC_000913/4214256‑4214434

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 23 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

GATTACTGCCGATCAGCTGCTGGCACCTTGTGATGGTGAACGCACCGAAGAAGGTATGCGCGCCAACATTCGCGTGGCTGTGCAGTACATCGAAGCGTGGATCTCTGGCAACGGCTGTGTGCCGATTTATGGCCTGATGGAAGATGCGGCGACGGCTGAAATTTCCCGTACCTCGATCTGGCAGTG  >  NC_000913/4214253‑4214438

GATTACTGCCGATCAGCTGCTGGCACCTTGTGATGGTGAACGCACCGAAGAAGGTATGCGCGCCAACATTCGCGTGGCTGTGCAGTACATCGAG                                                                                              >  SRR4302535.1777568/1‑94 (MQ=60)

GATTACTGCCGATCAGCTGCTGGCACCTTGTGATGGTGAACGCACCGAAGAAGGTATGCGCGCCAACATTCGCGTGGCTGTGCAGTACATCGAG                                                                                              <  SRR4302535.2217423/94‑1 (MQ=60)

GATTACTGCCGATCAGCTGCTGGCACCTTGTGATGGTGAACGCACCGAAGAAGGTATGCGCGCCAACATTCGCGTGGCTGTGCAGTACATCGAG                                                                                              <  SRR4302535.2355865/94‑1 (MQ=60)

GATTACTGCCGATCAGCTGCTGGCACCTTGTGATGGTGAACGCACCGAAGAAGGTATGCGCGCCAACATTCGCGTGGCTGTGCAGTACATCGAG                                                                                              >  SRR4302535.2574514/1‑94 (MQ=60)

 ATTACTGCCGATCAGCTGCTGGCACCTTGTGATGGTGAACGCACCGAAGAAGGTATGCGCGCCAACATTCGCGTGGCTGTGCAGTACATCGAGG                                                                                             <  SRR4302535.627161/94‑1 (MQ=60)

   TACTGCCGATCAGCTGCTGGCACCTTGTGATGGTGAACGCACCGAAGAAGGTATGCGCGCCAACATTCGCGTGGCTGTGCAGTACATCGAGGCG                                                                                           >  SRR4302535.3424874/1‑94 (MQ=60)

     CTGCCGATCAGCTGCTGGCACCTTGTGATGGTGAACGCACCGAAGAAGGTATGCGCGCCAACATTCGCGTGGCTGTGCAGTACATCGAGGCGTG                                                                                         <  SRR4302535.2172759/94‑1 (MQ=60)

      TGCCTATCAGCTGCTGGCACCTTGTGATGGTGAACGCACCGAAGAAGGTATGCGCGCCAACATTCGCGTGGCTGTGCAGTACATCGAGGCGTGG                                                                                        <  SRR4302535.1215728/94‑1 (MQ=60)

      TGCCGATCAGCTGCTGGCACCTTGTGATGGTGAACGCACCGAAGAAGGTATGCGCGCCAACATTCGCGTGGCTGTGCAGTACATCGAGGCGTGG                                                                                        <  SRR4302535.4076579/94‑1 (MQ=60)

       GCCGATCAGCTGCTGGCACCTTGTGATGGTGAACGCACCGAAGAAGGTATGCGCGCCAACATTCGCGTGGCTGTGCAGTACATCGAGGCGTGGA                                                                                       <  SRR4302535.2309857/94‑1 (MQ=60)

        CCGATCAGCTGCTGGCACCTTGTGATGGTGAACGCACCGAAGAAGGTATGCGCGCCAACATTCGCGTGGCTGTGCAGTACATCGAGGCGTGGAT                                                                                      >  SRR4302535.2583066/1‑94 (MQ=60)

        CCGATCAGCTGCTGGCACCTTGTGATGGTGAACGCACCGAAGAAGGTATGCGCGCCAACATTCGCGTGGCTGTGCAGTACATCGAGGCGTGGAT                                                                                      <  SRR4302535.3110739/94‑1 (MQ=60)

         CGATCAGCTGCTGGCACCTTGTGATGGTGAACGCACCGAAGAAGGTATGCGCGCCAACATTCGCGTGGCTGTGCAGTACATCGAGGCGTGGATC                                                                                     >  SRR4302535.3467882/1‑94 (MQ=60)

         CGATCAGCTGCTGGCACCTTGTGATGGTGAACGCACCGAAGAAGGTATGCGCGCCAACATTCGCGTGGCTGTGCAGTACATCGAGGCGTGGATC                                                                                     <  SRR4302535.3435462/94‑1 (MQ=60)

          GATCAGCTGCTGGCACCTTGTGATGGTGAACGCACCGAAGAAGGTATGCGCGCCAACATTCGCGTGGCTGTGCAGTACATCGAGGCGTGGATCT                                                                                    >  SRR4302535.87654/1‑94 (MQ=60)

          GATCAGCTGCTGGCACCTTGTGATGGTGAACGCACCGAAGAAGGTATGCGCGCCAACATTCGCGTGGCTGTGCAGTACATCGAGGCGTGGATCT                                                                                    <  SRR4302535.2824812/94‑1 (MQ=60)

             CAGCTGCTGGCACCTTGTGATGGTGAACGCACCGAAGAAGGTATGCGCGCCAACATTCGCGTGGCTGTGCAGTACATCGAGGCGTGGATCTCTG                                                                                 <  SRR4302535.147113/94‑1 (MQ=60)

             CAGCTGCTGGCACCTTGTGATGGTGAACGCACCGAAGAAGGTATGCGCGCCAACATTCGCGTGGCTGTGCAGTACATCGAGGCGTGGATCTCTG                                                                                 <  SRR4302535.1574797/94‑1 (MQ=60)

              AGCTGCTGGCACCTTGTGATGGTGAACGCACCGAAGAAGGTATGCGCGCCAACATTCGCGTGGCTGTGCAGTACATCGAGGCGTGGATCTCTGG                                                                                >  SRR4302535.4289010/1‑94 (MQ=60)

              AGCTGCTGGCACCTTGTGATGGTGAACGCACCGAAGAAGGTATGCGCGCCAACATTCGCGTGGCTGTGCAGTACATCGAGGCGTGGATCTCTGG                                                                                >  SRR4302535.114381/1‑94 (MQ=60)

                CTGCTGGCACCTTGTGATGGTGAACGCACCGAAGAAGGTATGCGCGCCAACATTCGCGTGGCTGTGCAGTACATCGAGGCGTGGATCTCTGGCA                                                                              >  SRR4302535.1050425/1‑94 (MQ=60)

                CTGCTGGCACCTTGTGATGGTGAACGCACCGAAGAAGGTATGCGCGCCAACATTCGCGTGGCTGTGCAGTACATCGAGGCGGGGATCTCTGGCA                                                                              >  SRR4302535.3312519/1‑94 (MQ=60)

                CTGCTGGCACCTTGTGATGGTGAACGCACCGAAGAAGGTATGCGAGCCAACATTCGCGTGGCTGTGCAGTACATCGAGGCGTGGATCTCTGGCA                                                                              >  SRR4302535.406780/1‑94 (MQ=60)

                 TGCTGGCACCTTGTGATGGTGAACGCACCGAAGAAGGTATGCGCGCCAACATTCGCGTGGCTGTGCAGTACATCGAGGCGTGGATCTCTGGCAA                                                                             <  SRR4302535.2815596/94‑1 (MQ=60)

                 TGCTGGCACCTTGTGATGGTGAACGCACCGAAGAAGGTATGCGCGCCAACATTCGCGTGGCTGTGCAGTACATCGAGGCGTGGATCTCTGGCAA                                                                             >  SRR4302535.2202783/1‑94 (MQ=60)

                  GCTGGCACCTTGTGATGGTGAACGCACCGAAGAAGGTATGCGCGCCAACATTCGCGTGGCTGTGCAGTACATCGAGGCGTGGATCTCTGGCAAC                                                                            <  SRR4302535.3817864/94‑1 (MQ=60)

                   CTGGCACCTTGTGATGGTGAACGCACCGAAGAAGGTATGCGCGCCAACATTCGCGTGGCTGTGCAGTACATCGAGGCGTGGATCTCTGGCAACG                                                                           >  SRR4302535.278293/1‑94 (MQ=60)

                   CTGGCACCTTGTGATGGTGAACGCACCGAAGAAGGTATGCGCGCCAACATTCGCGTGGCTGTGCAGTACATCGAGGCGTGGATCTCTGGCAACG                                                                           <  SRR4302535.4357134/94‑1 (MQ=60)

                   CTGGCACCTTGTGATGGTGAACGCACCGAAGAAGGTATGCGCGCCAACATTCGCGTGGCTGTGCAGTACATCGAGGCGTGGATCTCTGGCAACG                                                                           <  SRR4302535.92269/94‑1 (MQ=60)

                    TGGCACCTTGTGATGGTGAACGCACCGAAGAAGGTATGCGCGCCAACATTCGCGTGGCTGTGCAGTACATCGAGGCGTGGATCTCTGGCAACGG                                                                          >  SRR4302535.1193881/1‑94 (MQ=60)

                    TGGCACCTTGTGATGGTGAACGCACCGAAGAAGGTATGCGCGCCAACATTCGCGTGGCTGTGCAGTACATCGAGGCGTGGATCTCTGGCAACGG                                                                          <  SRR4302535.1367054/94‑1 (MQ=60)

                    TGGCACCTTGTGATGGTGAACGCACCGAAGAAGGTATGCGCGCCAACATTCGCGTGGCTGTGCAGTACATCGAGGCGTGGATCTCTGGCAACGG                                                                          <  SRR4302535.2149863/94‑1 (MQ=60)

                     GGCACCTTGTGATGGTGAACGCACCGAAGAAGGTATGCGCGCCAACATTCGCGTGGCTGTGCAGTACATCGAGGCGTGGATCTCTGGCAACGGC                                                                         <  SRR4302535.438200/94‑1 (MQ=60)

                     GGCACCTTGTGATGGTGAACGCACCGAAGAAGGTATGCGCGCCAACATTCGCGTGGCTGTGCAGTACATCGAGGCGTGGATCTCTGGCAACGGC                                                                         >  SRR4302535.435156/1‑94 (MQ=60)

                      GCACCTTGTGATGGTGAACGCACCGAAGAAGGTATGCGCGCCAACATTCGCGTGCCTGTGCAGTACATCGAGGCGTGGATCTCTGGCAcagtct                                                                        >  SRR4302535.43369/1‑88 (MQ=60)

                       CACCTTGTGATGGTGAACGCACCGAAGAAGGTATGCGCGCCAACATTCGCGTGGCTGTGCAGTACATCGAGGCGTGGATCTCTGGCAACGGCTG                                                                       >  SRR4302535.1278979/1‑94 (MQ=60)

                        ACCTTGTGATGGTGAACGCACCGAAGAAGGTATGCGCGCCAACATTCGCGTGGCTGTGCAGTACATCGAGGCGTGGATCTCTGGCAACGGCTGT                                                                      >  SRR4302535.2451245/1‑94 (MQ=60)

                        ACCTTGTGATGGTGAACGCACCGAAGAAGGTATGCGCGCCAACATTCGCGTGGCTGTGCAGTACATCGAGGCGTGGATCTCTGGCAACGGCTGT                                                                      >  SRR4302535.2173444/1‑94 (MQ=60)

                         CCTTGTGATGGTGAACGCACCGAAGAAGGTATGCGCGCCAACATTCGCGTGGCTGTGCAGTACATCGAGGCGTGGATCTCTGGCAACGGCTGTG                                                                     >  SRR4302535.3477211/1‑94 (MQ=60)

                          CTTGTGATGGTGAACGCACCGAAGAAGGTATGCGCGCCAACATTCGCGTGGCTGTGCAGTACATCGAGGCGTGGATCTCTGGCAACGGCTGTGT                                                                    >  SRR4302535.1839742/1‑94 (MQ=60)

                          CTTGTGATGGTGAACGCACCGAAGAAGGTATGCGCGCCAACATTCGCGTGGCTGTGCAGTACATCGAGGCGTGGATCTCTGGCAACGGCTGTGT                                                                    >  SRR4302535.617815/1‑94 (MQ=60)

                          CTTGTGATGGTGAACGCACCGAAGAAGGTATGCACGCCAACATTCGCGTGGCTGTGCAGTACATCGAGGCGTGGATCTCTGGCAACGGCTGTGT                                                                    <  SRR4302535.2165285/94‑1 (MQ=60)

                           TTGTGATGGTGAACGCACCGAAGAAGGTATGCGCGCCAACATTCGCGTGGCTGTGCAGTACATCGAGGCGTGGATCTCTGGCAACGGCTGTGTG                                                                   >  SRR4302535.1322830/1‑94 (MQ=60)

                           TTGTGATGGTGAACGCACCGAAGAAGGTATGCGCGCCAACATTCGCGTGGCTGTGCAGTACATCGAGGCGTGGATCTCTGGCAACGGCTGTGTG                                                                   <  SRR4302535.2944604/94‑1 (MQ=60)

                           TTGTGATGGTGAACGCACCGAAGAAGGTATGCGCGCCAACATTCGCGTGGCTGTGCAGTACATCGAGGCGTGGATCTCTGGCAACGGCTGTGTG                                                                   >  SRR4302535.1524976/1‑94 (MQ=60)

                           TTGTGATGGTGAACGCACCGAAGAAGGTATGCGCGCCAACATTCGCGTGGCTGTGCAGTACATCGAGGCGTGGATCTCTGGCAACGGCTGTGTG                                                                   <  SRR4302535.2326127/94‑1 (MQ=60)

                            TGTGATGGTGAACGCACCGAAGAAGGTATGCGCGCCAACATTCGCGTGGCTGTGCAGTACATCGAGGCGTGGATCTCTGGCAACGGCTGTGTGC                                                                  >  SRR4302535.1713232/1‑94 (MQ=60)

                                ATGGTGAACGCACCGAAGAAGGTATGCGCGCCAACATTCGCGTGGCTGTGCAGTACATCGAGGCGTGGATCTCTGGCAACGGCTGTGTGCCGAT                                                              <  SRR4302535.2111796/94‑1 (MQ=60)

                                 TGGTGAACGCACCGAAGAAGGTATGCGCGCCAACATTCGCGTGGCTGTGCAGTACATCGAGGCGTGGATCTCTGGCAACGGCTGTGTGCCGATT                                                             >  SRR4302535.3720095/1‑94 (MQ=60)

                                   GTGAACGCACCGAAGAAGGTATGCGCGCCAACATTCGCGTGGCTGTGCAGTACATCGAGGCGTGGATCTCTGGCAACGGCTGTGTGCCGATTTA                                                           <  SRR4302535.237416/94‑1 (MQ=60)

                                   GTGAACGCACCGAAGAAGGTATGCGCGCCAACATTCGCGTGGCTGTGCAGTACATCGAGGCGTGGATCTCTGGCAACGGCTGTGTGCCGATTTA                                                           >  SRR4302535.864815/1‑94 (MQ=60)

                                   GTGAACGCACCGAAGAAGGTATGCGCGCCAACATTCGCGTGGCTGTGCAGTACATCGAGGCGTGGATCTCTGGCAACGGCTGTGTGCCGAGTTA                                                           >  SRR4302535.1758279/1‑94 (MQ=60)

                                     GAACGCACCGAAGAAGGTATGCGCGCCAACATTCGCGTGGCTGTGCAGTACATCGAGGCGTGGATCTCTGGCAACGGCTGTGTGCCGATTTATG                                                         <  SRR4302535.3602400/94‑1 (MQ=60)

                                      AACGCACCGAAGAAGGTATGCGCGCCAACATTCGCGTGGCTGTGCAGTACATCGAGGCGTGGATCTCTGGCAACGGCTGTGTGCCGATTTATGG                                                        <  SRR4302535.4089388/94‑1 (MQ=60)

                                       ACGCACCGAAGAAGGTATGCGCGCCAACATTCGCGTGGCTGTGCAGTACATCGAGGCGTGGATCTCTGGCAACGGCTGTGTGCCGATTTATGGC                                                       <  SRR4302535.2491310/94‑1 (MQ=60)

                                       ACGCACCGAAGAAGGTATGCGCGCCAACATTCGCGTGGCTGTGCAGTACATCGAGGCGTGGATCTCTGGCAACGGCTGTGTGCCGATTTATGGC                                                       <  SRR4302535.3178280/94‑1 (MQ=60)

                                         GCACCGAAGAAGGTATGCGCGCCAACATTCGCGTGGCTGTGCAGTACATCGAGGCGTGGATCTCTGGCAACGGCTGTGTGCCGATTTATGGCCT                                                     <  SRR4302535.2994990/94‑1 (MQ=60)

                                          CACCGAAGAAGGTATGCGCGCCAACATTCGCGTGGCTGTGCAGTACATCGAGGCGTGGATCTCTGGCAACGGCTGTGTGCCGATTTATGGCCTG                                                    <  SRR4302535.4710124/94‑1 (MQ=60)

                                           ACCGAAGAAGGTATGCGCGCCAACATTCGCGTGGCTGTGCAGTACATCGAGGCGTGGATCTCTGGCAACGGCTGTGTGCCGATTTATGGCCTGA                                                   >  SRR4302535.4634042/1‑94 (MQ=60)

                                            CCGAAGAAGGTATGCGCGCCAACATTCGCGTGGCTGTGCAGTACATCGAGGCGTGGATCTCTGGCAACGGCTGTGTGCCGATTTATGGCCTGAT                                                  <  SRR4302535.1289074/94‑1 (MQ=60)

                                             CGAAGAAGGTATGCGCGCCAACATTCGCGTGGCTGTGCAGTACATCGAGGCGTGGATCTCTGGCAACGGCTGTGTGCCGATTTATGGCCTGATG                                                 >  SRR4302535.391865/1‑94 (MQ=60)

                                             CGAAGAAGGTATGCGCGCCAACATTCGCGTGGCTGTGCAGTACATCGAGGCGTGGATCTCTGGCAACGGCTGTGTGCCGATTTATGGCCTGATG                                                 <  SRR4302535.811431/94‑1 (MQ=60)

                                             CGAAGAAGGTATGCGCGCCAACATTCGCGTGGCTGTGCAGTACATCGAGGCGTGGATCTCTGGCAACGGCTGTGTGCCGATTTATGGCCTGATG                                                 <  SRR4302535.2712774/94‑1 (MQ=60)

                                             CGAAGAAGGTATGCGCGCCAACATTCGCGTGGCTGTGCAGTACATCGAGGCGTGGATCTCTGGCAACGGCTGTGTGCCGATTTATGGCCTGATG                                                 <  SRR4302535.1959412/94‑1 (MQ=60)

                                              GAAGAAGGTATGCGCGCCAACATTCGCGTGGCTGTGCAGTACATCGAGGCGTGGATCTCTGGCAACGGCTGTGTGCCGATTTATGGCCTGATGG                                                >  SRR4302535.3717297/1‑94 (MQ=60)

                                              GAAGAAGGTATGCGCGCCAACATTCGCGTGGCTGTGCAGTACATCGAGGCGTGGATCTCTGGCAACGGCTGTGTGCCGATTTATGGCCTGATGG                                                <  SRR4302535.1707621/94‑1 (MQ=60)

                                              GAAGAAGGTATGCGCGCCAACATTCGCGTGGCTGTGCAGTACATCGAGGCGTGGATCTCTGGCAACGGCTGTGTGCCGATTTATGGCCTGATGG                                                <  SRR4302535.3850487/94‑1 (MQ=60)

                                               AAGAAGGTATGCGCGCCAACATTCGCGTGGCTGTGCAGTACATCGAGGCGTGGATCTCTGGCAACGGCTGTGTGCCGATTTATGGCCTGATGGA                                               <  SRR4302535.3767208/94‑1 (MQ=60)

                                               AAGAAGGTATGCGCGCCAACATTCGCGTGGCTGTGCAGTACATCGAGGCGTGGATCTCTGGCAACGGCTGTGTGCCGATTTATGGCCTGATGGA                                               >  SRR4302535.3113872/1‑94 (MQ=60)

                                               AAGAAGGTATGCGCGCCAACATTCGCGTGGCTGTGCAGTACATCGAGGCGTGGATCTCTGGCAACGGCTGTGTGCCGATTTATGGCCTGATGGA                                               <  SRR4302535.2423692/94‑1 (MQ=60)

                                                AGAAGGTATGCGCGCCAACATTCGCGTGGCTGTGCAGTACATCGAGGCGTGGATCTCTGGCAACGGCTGTGTGCCGATTTATGGCCTGATGGAA                                              >  SRR4302535.766103/1‑94 (MQ=60)

                                                AGAAGGTATGCGCGCCAACATTCGCGTGGCTGTGCAGTACATCGAGGCGTGGATCTCTGGCAACGGCTGTGTGCCGATTTATGGCCTGATGGAA                                              >  SRR4302535.739237/1‑94 (MQ=60)

                                                AGAAGGTATGCGCGCCAACATTCGCGTGGCTGTGCAGTACATCGAGGCGTGGATCTCTGGCAACGGCTGTGTGCCGATTTATGGCCTGATGGAA                                              >  SRR4302535.2415862/1‑94 (MQ=60)

                                                 GAAGGTATGCGCGCCAACATTCGCGTGGCTGTGCAGTACATCGAGGCGTGGATCTCTGGCAACGGCTGTGTGCCGATTTATGGCCTGATGGAAG                                             <  SRR4302535.3899898/94‑1 (MQ=60)

                                                 GAAGGTATGCGCGCCAACATTCGCGTGGCTGTGCAGTACATCGAGGCGTGGATCTCTGGCAACGGCTGTGTGCCGATTTATGGCCTGATGGAAG                                             >  SRR4302535.2575582/1‑94 (MQ=60)

                                                   AGGTATGCGCGCCAACATTCGCGTGGCTGTGCAGTACATCGAGGCGTGGATCTCTGGCAACGGCTGTGTGCCGATTTATGGCCTGATGGAAGAT                                           >  SRR4302535.578279/1‑94 (MQ=60)

                                                   AGGTATGCGCGCCAACATTCGCGTGGCTGTGCAGTACATCGAGGCGTGGATCTCTGGCAACGGCTGTGTGCCGATTTATGGCCTGATGGAAGAT                                           <  SRR4302535.3520888/94‑1 (MQ=60)

                                                   AGGTATGCGCGCCAACATTCGCGTGGCTGTGCAGTACATCGAGGCGTGGATCTCTGGCAACGGCTGTGTGCCGATTTATGGCCTGATGGAAGAT                                           >  SRR4302535.2121777/1‑94 (MQ=60)

                                                   AGGTATGCGCGCCAACATTCGCGTGGCTGTGCAGTACATCGAGGCGTGGATCTCTGGCAACGGCTGTGTGCCGATTTATGGCCTGATGGAAGAT                                           >  SRR4302535.3399992/1‑94 (MQ=60)

                                                    GGTATGCGCGCCAACATTCGCGTGGCTGTGCAGTACATCGAGGCGTGGATCTCTGGCAACGGCTGTGTGCCGATTTATGGCCTGATGGAAGATG                                          <  SRR4302535.4099434/94‑1 (MQ=60)

                                                     GTATGCGCGCCAACATTCGCGTGGCTGTGCAGTACATCGAGGCGTGGATCTCTGGCAACGGCTGTGTGCCGATTTATGGCCTGATGGAAGATGC                                         >  SRR4302535.4628416/1‑94 (MQ=60)

                                                     GTATGCGCGCCAACATTCGCGTGGCTGTGCAGTACATCGAGGCGTGGATCTCTGGCAACGGCTGTGTGCCGATTTATGGCCTGATGGAAGATGC                                         >  SRR4302535.272902/1‑94 (MQ=60)

                                                      TATGCGCGCCAACATTCGCGTGGCTGTGCAGTACATCGAGGCGTGGATCTCTGGCAACGGCTGTGTGCCGATTTATGGCCTGATGGAAGATGCG                                        <  SRR4302535.2566483/94‑1 (MQ=60)

                                                      TATGCGCGCCAACATTCGCGTGGCTGTGCAGTACATCGAGGCGTGGATCTCTGGCAACGGCTGTGTGCCGATTTATGGCCTGATGGAAGATGCG                                        <  SRR4302535.2853001/94‑1 (MQ=60)

                                                       ATGCGCGCCAACATTCGCGTGGCTGTGCAGTACATCGAGGCGTGGATCTCTGGCAACGGCTGTGTGCCGATTTATGGCCTGATGGAAGATGCGG                                       <  SRR4302535.451700/94‑1 (MQ=60)

                                                       ATGCGCGCCAACATTCGCGTGGCTGTGCAGTACATCGAGGCGTGGATCTCTGGCAACGGCTGTGTGCCGATTTATGGCCTGATGGAAGATGCGG                                       <  SRR4302535.171631/94‑1 (MQ=60)

                                                       ATGCGCGCCAACATTCGCGTGGCTGCGCAGTACATCGAGGCGTGGATCTCTGGCAACGGCTGTGTGCCGATTTATGGCCTGATGGAAGATGCGG                                       >  SRR4302535.2029292/1‑94 (MQ=60)

                                                         GCGCGCCAACATTCGCGTGGCTGTGCAGTACATCGAGGCGTGGATCTCTGGCAACGGCTGTGTGCCGATTTATGGCCTGATGGAAGATGCGGCG                                     <  SRR4302535.3964233/94‑1 (MQ=60)

                                                          CGCGCCAACATTCGCGTGGCTGTGCAGTACATCGAGGCGTGGATCTCTGGCAACGGCTGTGTGCCGATTTATGGCCTGATGGAAGATGCGGCGA                                    >  SRR4302535.860657/1‑94 (MQ=60)

                                                           GCGCCAACATTCGCGTGGCTGTGCAGTACATCGAGGCGTGGATCTCTGGCAACGGCTGTGTGCCGATTTATGGCCTGATGGAAGATGCGGCGAC                                   <  SRR4302535.1351757/94‑1 (MQ=60)

                                                           GCGCCAACATTCGCGTGGCTGTGCAGTACATCGAGGCGTGGATCTCTGGCAACGGCTGTGTGCCGATTTATGGCCTGATGGAAGATGCGGCGAC                                   <  SRR4302535.1571581/94‑1 (MQ=60)

                                                             GCCAACATTCGCGTGGCTGTGCAGTACATCGAGGCGTGGATCTCTGGCAACGGCTGTGTGCCGATTTATGGCCTGATGGAAGATGCGGCGACGG                                 >  SRR4302535.1076804/1‑94 (MQ=60)

                                                               CAACATTCGCGTGGCTGTGCAGTACATCGAGGCGTGGATCTCTGGCAACGGCTGTGTGCCGATTTATGGCCTGATGGAAGATGCGGCGACGGCT                               <  SRR4302535.4623314/94‑1 (MQ=60)

                                                               CAACATTCGCGTGGCTGTGCAGTACATCGAGGCGTGGATCTCTGGCAACGGCTGTGTGCCGATTTATGGCCTGATGGAAGATGCGGCGACGGCT                               >  SRR4302535.3404133/1‑94 (MQ=60)

                                                               CAACATTCGCGTGGCTGTGCAGTACATCGAGGCGTGGATCTCTGGCAACGGCTGTGTGCCGATTTATGGCCTGATGGAAGATGCGGCGACGGCT                               <  SRR4302535.1042771/94‑1 (MQ=60)

                                                                AACATTCGCGTGGCTGTGCAGTACATCGAGGCGTGGATCTCTGGCAACGGCTGTGTGCCGATTTATGGCCTGATGGAAGATGCGGCGACGGCTG                              >  SRR4302535.4081114/1‑94 (MQ=60)

                                                                AACATTCGCGTGGCTGTGCAGTACATCGAGGCGTGGATCTCTGGCAACGGCTGTGTGCCGATTTATGGCCTGATGGAAGATGCGGCGACGGCTG                              >  SRR4302535.2392737/1‑94 (MQ=60)

                                                                 ACATTCGCGTGGCTGTGCAGTACATCGAGGCGTGGATCTCTGGCAACGGCTGTGTGCCGATTTATGGCCTGATGGAAGATGCGGCGACGGCTGA                             <  SRR4302535.2428420/94‑1 (MQ=60)

                                                                   ATTCGCGTGGCTGTGCAGTACATCGAGGCGTGGATCTCTGGCAACGGCTGTGTGCCGATTTATGGCCTGATGGAAGATGCGGCGACGGCTGAAA                           >  SRR4302535.1153679/1‑94 (MQ=60)

                                                                   ATTCGCGTGGCTGTGCAGTACATCGAGGCGTGGATCTCTGGCAACGGCTGTGTGCCGATTTATGGCCTGATGGAAGATGCGGCGACGGCTGAAA                           >  SRR4302535.2166258/1‑94 (MQ=60)

                                                                   ATTCGCGTGGCTGTGCAGTACATCGAGGCGTGGATCTCTGGCAACGGCTGTGTGCCGATTTATGGCCTGATGGAAGATGCGGCGACGGCTGAAA                           >  SRR4302535.2871888/1‑94 (MQ=60)

                                                                   ATTCGCGTGGCTGTGCAGTACATCGAGGCGTGGATCTCTGGCAACGGCTGTGTGCCGATTTATGGCCTGATGGAAGATGCGGCGACGGCTGAAA                           >  SRR4302535.2660817/1‑94 (MQ=60)

                                                                    TTCGCGTGGCTGTGCAGTACATCGAGGCGTGGATCTCTGGCAACGGCTGTGTGCCGATTTATGGCCTGATGGAAGATGCGGCGACGGCTGAAAT                          <  SRR4302535.3674368/94‑1 (MQ=60)

                                                                     TCGCGTGGCTGTGCAGTACATCGAGGCGTGGATCTCTGGCAACGGCTGTGTGCCGATTTATGGCCTGATGGAAGATGCGGCGACGGCTGAAATT                         <  SRR4302535.4323888/94‑1 (MQ=60)

                                                                      CGCGTGGCTGTGCAGTACATCGAGGCGTGGATCTCTGGCAACGGCTGTGTGCCGATTTATGGCCTGATGGAAGATGCGGCGACGGCTGAAATTT                        >  SRR4302535.194033/1‑94 (MQ=60)

                                                                      CGCGTGGCTGTGCAGTACATCGAGGCGTGAATCTCTGGCAACGGCTGTGTGCCGATTTATGGCCTGATGGAAGATGCGGCGACGGCTGAAATTT                        <  SRR4302535.229740/94‑1 (MQ=60)

                                                                       GCGTGGCTGTGCAGTACATCGAGGCGTGGATCTCTGGCAACGGCTGTGTGCCGATTTATGGCCTGATGGAAGATGCGGCGACGGCTGAAATTTC                       >  SRR4302535.1247436/1‑94 (MQ=60)

                                                                       GCGTGGCTGTGCAGTACATCGAGGCGTGGATCTCTGGCAACGGCTGTGTGCCGATTTATGGCCTGATGGAAGATGCGGCGACGGCTGAAATTTC                       >  SRR4302535.2609725/1‑94 (MQ=60)

                                                                       GCGTGGCTGTGCAGTACATCGAGGCGTGGATCTCTGGCAACGGCTGTGTGCCGATTTATGGCCTGATGGAAGATGCGGAGACGGCTGAAATTTC                       >  SRR4302535.3379002/1‑94 (MQ=60)

                                                                        CGTGGCTGTGCAGTACATCGAGGCGTGGATCTCTGGCAACGGCTGTGTGCCGATTTATGGCCTGATGGAAGATGCGGCGACGGCTGAAATTTCC                      <  SRR4302535.2130020/94‑1 (MQ=60)

                                                                         GTGGCTGTGCAGTACATCGAGGCGTGGATCTCTGGCAACGGCTGTGTGCCGATTTATGGCCTGATGGAAGATGCGGCGACGGCTGAAATTTCCC                     <  SRR4302535.179246/94‑1 (MQ=60)

                                                                         GTGGCTGTGCAGTACATCGAGGCGTGGATCTCTGGCAACGGCTGTGTGCCGATTTATGGCCTGATGGAAGATGCGGCGACGGCTGAAATTTCCC                     <  SRR4302535.2574650/94‑1 (MQ=60)

                                                                          TGGCTGTGCAGTACATCGAGGCGTGTATCTCTGGCAACGGCTGTGTGCCGATTTATGGCCTGATGGAAGATGCGGCGACGGCTGAAATTTCCCG                    <  SRR4302535.832172/94‑1 (MQ=60)

                                                                          TGGCTGTGCAGTACATCGAGGCGTGGATCTCTGGCAACGGCTGTGTGCCGATTTATGGCCTGATGGAAGATGCGGCGACGGCTGAAATTTCCCG                    >  SRR4302535.1023293/1‑94 (MQ=60)

                                                                           GGCTGCGCAGTACATCGAGACGTGAATCTCTGGCAACGGCTGTGTGCCGATTTATGGCCTGATGGAAGATGCGGCGACGGCTGAAATTTCCCGT                   <  SRR4302535.4696437/94‑1 (MQ=60)

                                                                              TGTGCAGTACATCGAGGCGTGGATCTCTGGCAACGGCTGTGTGCCGATTTATGGCCTGATGGAAGATGCGGCGACGGCTGAAATTTCCCGTACC                <  SRR4302535.2716763/94‑1 (MQ=60)

                                                                                TGCAGTACATCGAGGCGTGGATCTCTGGCAACGGCTGTGTGCCGATTTATGGCCTGATGGAAGATGCGGCGACGGCTGAAATTTCCCGTACCTC              <  SRR4302535.3667134/94‑1 (MQ=60)

                                                                                 GCAGTACATCGAGGCGTGGATCTCTGGCAACGGCTGTGTGCCGATTTATGGCCTGATGGAAGATGCGGCGACGGCTGAAATTTCCCGTACCTCG             <  SRR4302535.727171/94‑1 (MQ=60)

                                                                                   AGTACATCGAGGCGTGGATCTCTGGCAACGGCTGTGTGCCGATTTATGGCCTGATGGAAGATGCGGCGACGGCTGAAATTTCCCGTACCTCGAT           >  SRR4302535.1885857/1‑94 (MQ=60)

                                                                                   AGTACATCGAGGCGTGGATCTCTGGCAACGGCTGTGTGCCGATTTATGGCCTGATGGAAGATGCGGCGACGGCTGAAATTTCCCGTACCTCGAT           >  SRR4302535.1525939/1‑94 (MQ=60)

                                                                                    GTACATCGAGGCGTGGATCTCTGGCAACGGCTGTGTGCCGATTTATGGCCTGATGGAAGATGCGGCGACGGCTGAAATTTCCCGTACCTCGATC          <  SRR4302535.3799683/94‑1 (MQ=60)

                                                                                     TACATCGAGGCGTGGATCTCTGGCAACGGCTGTGTGCCGATTTATGGCCTGATGGAAGATGCGGCGACGGCTGAAATTTCCCGTACCTCGATCT         <  SRR4302535.1634779/94‑1 (MQ=60)

                                                                                     TACATCGAGGCGTGGATCTCTGGCAACGGCTGTGTGCCGATTTATGGCCTGATGGAAGATGCGGCGACGGCTGAAATTTCCCGTACCTCGATCT         <  SRR4302535.2796757/94‑1 (MQ=60)

                                                                                     TACATCGAGGCGTGGATCTCTGGCAACGGCTGTGTGCCGATTTATGGCCTGATGGAAGATGCGGCGACGGCTGAAATTTCCCGTACCTCGATCT         >  SRR4302535.3413330/1‑94 (MQ=60)

                                                                                     TACATCGAGGCGTGGATCTCTGGCAACGGCTGTGTGCCGATTTATGGCCTGATGGAAGATGCGGCGACGGCTGAAATTTCCCGTACCTCGATCT         <  SRR4302535.1086416/94‑1 (MQ=60)

                                                                                     TACATCGAGGCGTGGATCTCTGGCAACGGCTGTGTGACGATTTATGGCCTGATGGAAGATGCGGCGACGGCTGAAATTTCCCGTACCTCGATCT         <  SRR4302535.691391/94‑1 (MQ=60)

                                                                                      TCATCGAGGCGTGGATCTCTGGCAACGGCTGTGTGCCGATTTATGGCCTGATGGAAGATGCGGCGACGGCTGAAATTTCCCGTACCTCGATCTG        <  SRR4302535.4491386/94‑1 (MQ=60)

                                                                                      ACATCGAGGCGTGGATCTCTGGCAACGGCTGTGTGCCGATTTATGGCCTGATGGAAGATGCGGCGACGGCTGAAATTTCCCGTACCTCGATCTG        <  SRR4302535.3868438/94‑1 (MQ=60)

                                                                                      ACATCGAGGCGTGGATCTCTGGCAACGGCTGTGTGCCGATTTATGGCCTGATGGAAGATGCGGCGACGGCTGAAATTTCCCGTACCTCGATCTG        >  SRR4302535.89189/1‑94 (MQ=60)

                                                                                       CATCGAGGCGTGGATCTCTGGCAACGGCTGTGTGCCGATTTATGGCCTGATGGAAGATGCGGCGACGGCTGAAATTTCCCGTACCTCGATCTGG       <  SRR4302535.4383042/94‑1 (MQ=60)

                                                                                        ATCGAGGCGTGGATCTCTGGCAACGGCTGTGTGCCGATTTATGGCCTGATGGAAGATGCGGCGACGGCTGAAATTTCCCGTACCTCGATCTGGC      >  SRR4302535.1573468/1‑94 (MQ=60)

                                                                                        ATCGAGGCGTGGATCTCTGGCAACGGCTGTGTGCCGATTTATGGCCTGATGGAAGATGCGGCGACGGCTGAAATTTCCCGTACCTCGATCTGGC      >  SRR4302535.1588221/1‑94 (MQ=60)

                                                                                         TCGAGGCGTGGATCTCTGGCAACGGCTGTGTGCCGATTTATGGCCTGATGGAAGATGCGGCGACGGCTGAAATTTCCCGTACCTCGATCTGGCA     <  SRR4302535.3894091/94‑1 (MQ=60)

                                                                                         TCGAGGCGTGGATCTCTGGCAACGGCTGTGTGCCGATTTATGGCCTGATGGAAGATGCGGCGACGGCTGAAATTTCCCGTACCTCGATCTGGCA     <  SRR4302535.4332746/94‑1 (MQ=60)

                                                                                           GAGGCGTGGATCTCTGGCAACGGCTGTGTGCCGATTTATGGCCTGATGGAAGATGCGGCGACGGCTGAAATTTCCCGTACCTCGATCTGGCAGT   <  SRR4302535.3646624/94‑1 (MQ=60)

                                                                                           GAGGCGTGGATCTCTGGCAACGGCTGTGTGCCGATTTATGGCCTGATGGAAGATGCGGCGACGGCTGAAATTTCCCGTACCTCGATCTGGCAGT   <  SRR4302535.1977458/94‑1 (MQ=60)

                                                                                           GAGGCGTGGATCTCTGGCAACGGCTGTGTGCCGATTTATGGCCTGATGGAAGATGCGGCGACGGCTGAAATTTCCCGTACCTCGATCTGGCAGT   >  SRR4302535.901173/1‑94 (MQ=60)

                                                                                            AGGCGTGGATCTCTGGCAACGGCTGTGTGCCGATTTATGGCCTGATGGAAGATGCGGCGACGGCTGAAATTTCCCGTACCTCGATCTGGCAGTG  <  SRR4302535.3357239/94‑1 (MQ=60)

GATTACTGCCGATCAGCTGCTGGCACCTTGTGATGGTGAACGCACCGAAGAAGGTATGCGCGCCAACATTCGCGTGGCTGTGCAGTACATCGAAGCGTGGATCTCTGGCAACGGCTGTGTGCCGATTTATGGCCTGATGGAAGATGCGGCGACGGCTGAAATTTCCCGTACCTCGATCTGGCAGTG  >  NC_000913/4214253‑4214438

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 17 ≤` `ATCG/ATCG` `< 28 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`