Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 1696 279.3 15874187 97.8% 15524954 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,214,346 A→G E430E (GAA→GAG)  aceB → malate synthase A

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,214,3460AG99.2% 1173.9 / ‑2.7 376E430E (GAA→GAG) aceBmalate synthase A
Reads supporting (aligned to +/- strand):  ref base A (1/2);  new base G (182/191);  total (183/193)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.30e-01

Only 100 of 381 total aligned reads displayed.

TTACTGCCGATCAGCTGCTGGCACCTTGTGATGGTGAACGCACCGAAGAAGGTATGCGCGCCAACATTCGCGTGGCTGTGCAGTACATCGAAGCGTGGATCTCTGGCAACGGCTGTGTGCCGATTTATGGCCTGATGGAAGATGCGGCGACGGCTGAAATTTCCCGTACCTCGATCTGGCA  >  NC_000913/4214255‑4214435
                                                                                           |                                                                                         
  aCTGCCGATCAGCTGCTGGCACCTTGTGATGGTGAACGCACCGAAGAAGGTATGCGCGCCAACATTCGCGTGGCTGTGCAGTACATCGAGGCg                                                                                        >  1:6578063/1‑93 (MQ=255)
    tGCCGATCAGCTGCTGGCACCTTGTGATGGTGAACGCACCGAAGAAGGTATGCGCGCCAACATTCGCGTGGCTGTGCAGTACATCGAGGCGTg                                                                                      >  2:1063244/1‑93 (MQ=255)
         aTCAGCTGCTGGCACCTTGTGATGGTGAACGCACCGAAGAAGGTATGCGCGCCAACATTCGCGTGGCTGTGCAGTACATCGAGGCGTGGAtct                                                                                 >  2:2134481/1‑93 (MQ=255)
         aTCAGCTGCTGGCACCTTGTGATGGTGAACGCACCGAAGAAGGTATGCGCGCCAACATTCGCGTGGCTGTGCAGTACATCGAGGCGTGGAtct                                                                                 >  1:6651008/1‑93 (MQ=255)
         aTCAGCTGCTGGCACCTTGTGATGGTGAACGCACCGAAGAAGGTATGCGCGCCAACATTCGCGTGGCTGTGCAGTACATCGAGGCGTGGAtct                                                                                 >  1:2695707/1‑93 (MQ=255)
          tCAGCTGCTGGCACCTTGTGATGGTGAACGCACCGAAGAAGGTATGCGCGCCAACATTCGCGTGGCTGTGCAGTACATCGAGGCGTGGAtctc                                                                                >  2:5293256/1‑93 (MQ=255)
          tCAGCTGCTGGCACCTTGTGATGGTGAACGCACCGAAGAAGGTATGCGCGCCAACATTCGCGTGGCTGTGCAGTACATCGAGGCGTGGAtctc                                                                                <  1:1894248/93‑1 (MQ=255)
               tgctgGCACCTTGTGATGGTGAACGCACCGAAGAAGGTATGCGCGCCAACATTCGCGTGGCTGTGCAGTACATCGAGGCGTGGATCTCTGGCa                                                                           >  1:1683978/1‑93 (MQ=255)
                gctgGCACCTTGTGATGGTGAACGCACCGAAGAAGGTATGCGCGCCAACATTCGCGTGGCTGTGCAGTACATCGAGGCGTGGATCTCTGGCaa                                                                          <  1:1986473/93‑1 (MQ=255)
                 ctgGCACCTTGTGATGGTGAACGCACCGAAGAAGGTATGCGCGCCAACATTCGCGTGGCTGTGCAGTACATAGAGGCGTGGATCTCTGGCAAc                                                                         <  1:1402731/93‑1 (MQ=255)
                   ggCACCTTGTGATGGTGAACGCACCGAAGAAGGTATGCGCGCCAACATTCGCGTGGCTGTGCAGTACATCGAGGCGTGGATCTCTGGCAACgg                                                                       >  2:4257980/1‑93 (MQ=255)
                     cACCTTGTGATGGTGAACGCACCGAAGAAGGTATGCGCGCCAACATTCGCGTGGCTGTGCAGTACATCGAGGCGTGGATCTCTGGCAACGGCt                                                                     <  1:4614429/93‑1 (MQ=255)
                     cACCTTGTGATGGTGAACGCACCGAAGAAGGTATGCGCGCCAACATTCGCGTGGCTGTGCAGTACATCGAGGCGTGGATCTCTGGCAACGGCt                                                                     <  2:4226785/93‑1 (MQ=255)
                        cTTGTGATGGTGAACGCACCGAAGAAGGTATGCGCGCCAACATTCGCGTGGCTGTGCAGTCCATCGAGGCGTGGATCTCTGGCAACGGCtgtg                                                                  >  2:4998515/1‑93 (MQ=255)
                        cTTGTGATGGTGAACGCACCGAAGAAGGTATGCGCGCCAACATTCGCGTGGCTGTGCAGTACATCGAGGCGTGTATCTCTGGCAACGGCtgtg                                                                  <  1:1439171/93‑1 (MQ=255)
                        cTTGTGATGGTGAACGCACCGAAGAAGGTATGCGCGCCAACATTCGCGTGGCTGTGCAGTACATCGAGGCGTGGATCTCTGGCAACGGCtgtg                                                                  >  1:4023329/1‑93 (MQ=255)
                        cTTGTGATGGTGAACGCACCGAAGAAGGTATGCGCGCCAACATTCGCGTGGCTGTGCAGTACATCGAGGCGTGGATCTCTGGCAACGGCtgtg                                                                  <  1:5096375/93‑1 (MQ=255)
                        cTTGTGATGGTGAACGCACCGAAGAAGGTATGCGCGCCAACATTCGCGTGGCTGTGCAGTACATCGAGGCGTGGATCTCTGGCAACGGCtgtg                                                                  <  2:6757671/93‑1 (MQ=255)
                         ttGTGATGGTGAACGCACCGAAGAAGGTATGCGCGCCAACATTCGCGTGGCTGTGCAGTACATCGAGGCGTGGATCTCTGGCAACGGCtgtgt                                                                 >  1:3300284/1‑93 (MQ=255)
                         ttGTGATGGTGAACGCACCGAAGAAGGTATGCGCGCCAACATTCGCGTGGCTGTGCAGTACATCGAGGCGTGGATCTCTGGCAACGGCtgtgt                                                                 <  1:7870137/93‑1 (MQ=255)
                         ttGTGATGGTGAACGCACCGAAGAAGGTATGCGCGCCAACATTCGCGTGGCTGTGCAGTACATCGAGGCGTGGATCTCTGGCAACGGCtgtgt                                                                 <  2:728587/93‑1 (MQ=255)
                           gtgATGGTGAACGCACCGAAGAAGGTATGCGCGCCAACATTCGCGTGGCTGTGCAGTACATCGAGGCGTGGATCTCTGGCAACGGCTGTGTGc                                                               >  1:1284238/1‑93 (MQ=255)
                           gtgATGGTGAACGCACCGAAGAAGGTATGCGCGCCAACATTCGCGTGGCTGTGCAGTACATCGAGGCGTGGATCTCTGGCAACGGCTGTGTGc                                                               <  1:5696514/93‑1 (MQ=255)
                           gtgATGGTGAACGCACCGAAGAAGGTATGCGCGCCAACATTCGCGTGGCTGTGCAGTACATCGAGGCGTGGATCTCTGGCAACGGCTGTGTGc                                                               <  1:5485105/93‑1 (MQ=255)
                            tgATGGTGAACGCACCGAAGAAGGTATGCGCGCCAACATTCGCGTGGCTGTGCAGTACATCGAGGCGTGGATCTCTGGCAACGGCTGTGTGcc                                                              >  2:1236211/1‑93 (MQ=255)
                            tgATGGTGAACGCACCGAAGAAGGTATGCGCGCCAACATTCGCGTGGCTGTGCAGTACATCGAGGCGTGGATCTCTGGCAACGGCTGTGTGcc                                                              >  1:3935303/1‑93 (MQ=255)
                            tgATGGTGAACGCACCGAAGAAGGTATGCGCGCCAACATTCGCGTGGCTGTGCAGTACATCGAGGCGTGGATCTCTGGCAACGGCTGTGTGcc                                                              >  2:2711730/1‑93 (MQ=255)
                              aTGGTGAACGCACCGAAGAAGGTATGCGCGCCAACATTCGCGTGGCTGTGCAGTGCATCGAGGCGTGGATCTCTGGCAACGGCTGTGTGCCGa                                                            <  1:6857372/93‑1 (MQ=255)
                                ggTGAACGCACCGAAGAAGGTATGCGCGCCAACTTTCGCGTGGCTGTGCAGTACATCGAGGCGTGGATCTCTGGCAACGGCTGTGTGCCGAtt                                                          <  1:7605809/93‑1 (MQ=255)
                                  tGAACGCACCGAAGAAGGTATGCGCGCCAACATTCGCGTGGCTGTGCAGTACATCGAGGCGTGGATCTCTGGCAACGGCTGTGTGCCGATTTa                                                        <  2:4630608/93‑1 (MQ=255)
                                  tGAACGCACCGAAGAAGGTATGCGCGCCAACATTCGCGTGGCTGTGCAGTACATCGAGGCGTGGATCTCTGGCAACGGCTGTGTGCCGATTTa                                                        >  1:5667512/1‑93 (MQ=255)
                                  tGAACGCACCGAAGAAGGTATGCGCGCCAACATTCGCGTGGCTGTGCAGTACATCGAGGCGTGGATCTCTGGCAACGGCTGTGTGCCGATTTa                                                        <  1:1031530/93‑1 (MQ=255)
                                  tGAACGCACCGAAGAAGGTATGCGCGCCAACATTCGCGTGGCTGTGCAGTACATCGAGGCGTGGATCTCTGGCAACGGCTGTGTGCCGATTTa                                                        <  1:3034543/93‑1 (MQ=255)
                                  tGAACGCACCGAAGAAGGTATGCGCGCCAACATTCGCGTGGCTGTGCAGTACATCGAGGCGTGGATCTCTGGCAACGGCTGTGTGCCGATTTa                                                        <  1:4827184/93‑1 (MQ=255)
                                  tGAACGCACCGAAGAAGGTATGCGCGCCAACATTCGCGTGGCTGTGCAGTACATCGAGGCGTGGATCTCTGGCAACGGCTGTGTGCCGATTTa                                                        <  1:36280/93‑1 (MQ=255)
                                   gAACGCACCGAAGAAGGTATGCGCGCCAACATTCGCGTGGCTGTGCAGTACATCGAGGCGTGGATCTCTGGCAACGGCTGTGTGCCGATTTAt                                                       <  2:7402305/93‑1 (MQ=255)
                                   gAACGCACCGAAGAAGGTATGCGCGCCAACATTCGCGTGGCTGTGCAGTACATCGAGGCGTGGATCTCTGGCAACGGCTGTGTGCCGATTTAt                                                       <  2:7181773/93‑1 (MQ=255)
                                    aaCGCACCGAAGAAGGTATGCGCGCCAACATTCGCGTGGCTGTGCAGTACATCGAGGCGTGGATCTCTGGCAACGGCTGTGTGCCGATTTATg                                                      <  1:4408868/93‑1 (MQ=255)
                                      cGCACCGAAGAAGGTATGCGCGCCAACATTCGCGTGGCTGTGCAGTACATCGAGGCGTGGATCTCTGGCAACGGCTGTGTGCCGATTTATGGc                                                    <  1:6005228/93‑1 (MQ=255)
                                      cGCACCGAAGAAGGTATGCGCGCCAACATTCGCGTGGCTGTGCAGTACATCGAGGCGTGGATCTCTGGCAACGGCTGTGTGCCGATTTATGGc                                                    >  2:6084889/1‑93 (MQ=255)
                                       gCACCGAAGAAGGTATGCGCGCCAACATTCGCGTGGCTTTGCAGTACATCGAGGCGTGGATCTCTGGCAACGGCTGTGTGCCGATTTATGGcc                                                   >  1:5011100/1‑93 (MQ=255)
                                         aCCGAAGAAGGTATGCGCGCCAACATTCGCGTGGCTGTGCAGTACATCGAGGCGTGGATCTCTGGCAACGGCTGTGTGCCGATTTATGGCCTg                                                 <  1:7476743/93‑1 (MQ=255)
                                          ccGAAGAAGGTATGCGCGCCAACATTCGCGTGGCTGTGCAGTACATCGAGGCGTGGATCTCTGGCAACGGCTGTGTGCCGATTTATGGCCTGa                                                <  2:1318998/93‑1 (MQ=255)
                                           cGAAGAAGGTATGCGCGCCAACATTCGCGTGGCTGTGCAGTACATCGAGGCGTGGATCTCTGGCAACGGCTGTGTGCCGATTTATGGCCTGAt                                               <  1:4538112/93‑1 (MQ=255)
                                             aagaagGTATGCGCGCCAACATTCGCGTGGCTGTGCAGTACATCGAGGCGTGGATCTCTGGCAACGGCTGTGTGCCGATTTATGGCCTGATgg                                             <  2:6733088/93‑1 (MQ=255)
                                              agaagGTATGCGCGCCAACATTCGCGTGGCTGTGCAGTACATCGAGGCGTGGATCTCTGGCAACGGCTGTGTGCCGATTTATGGCCTGATGGa                                            >  2:2967799/1‑93 (MQ=255)
                                              agaagGTATGCGCGCCAACATTCGCGTGGCTGTGCAGTACATCGAGGCGTGGATCTCTGGCAACGGCTGTGTGCCGATTTATGGCCTGATGGa                                            <  2:5103860/93‑1 (MQ=255)
                                               gaagGTATGCGCGCCAACATTCGCGTGGCTGTGCAGTACATCGAGGCGTGGATCTCTGGCAACGGCTGTGTGCCGATTTATGGCCTGATGGaa                                           <  1:6942856/93‑1 (MQ=255)
                                                aagGTATGCGCGCCAACATTCGCGTGGCTGTGCAGTACATCGAGGCGTGGATCTCTGGCAACGGCTGTGTGCCGATTTATGGCCTGATGGAAg                                          <  1:4229752/93‑1 (MQ=255)
                                                aagGTATGCGCGCCAACATTCGCGTGGCTGTGCAGTACATCGAGGCGTGGATCTCTGGCAACGGCTGTGTGCCGATTTATGGCCTGATGGAAg                                          <  2:388527/93‑1 (MQ=255)
                                                aagGTATGCGCGCCAACATTCGCGTGGCTGTGCAGTACATCGAGGCGTGGATCTCTGGCAACGGCTGTGTGCCGATTTATGGCCTGATGGAAg                                          <  1:371654/93‑1 (MQ=255)
                                                 agGTATGCGCGCCAACATTCGCGTGGCTGTGCAGTACCTCGAGGCGTGGATCTCTGGCAACGGCTGTGTGCCGAATTATGGCCTGATGGAAGa                                         <  2:7680201/93‑1 (MQ=255)
                                                  ggTATGCGCGCCAACATTCGCGTGGCTGTGCAGTACATCGAGGCGTGGATCTCTGGCAACGGCTGTGTGCCGATTTATGGCCTGATGGAAGAt                                        >  1:712100/1‑93 (MQ=255)
                                                  ggTATGCGCGCCAACATTCGCGTGGCTGTGCAGTACATCGAGGCGTGGATCTCTGGCAACGGCTGTGTGCCGATTTATGGCCTGATGGAAGAt                                        <  2:6694850/93‑1 (MQ=255)
                                                  ggTATGCGCGCCAACATTCGCGTGGCTGTGCAGTACATCGAGGCGTGGATCTCTGGCAACGGCTGTGTGCCGATTTATGGCCTGATGGAAGAt                                        <  2:2347934/93‑1 (MQ=255)
                                                   gTATGCGCGCCAACATTCGCGTGGCTGTGCAGTACATCGAGGCGTGGATCTCTGGCAACGGCTGTGTGCCGATTTATGGCCTGATGGAAGATg                                       <  1:7057182/93‑1 (MQ=255)
                                                    tATGCGCGCCAACATTCGCGTGGCTGTGCAGTACATCGAGGCGTGGATCTCTGGCAACGGCTGTGTGCCGATTTATGGCCTGATGGAAGATgc                                      <  1:5846374/93‑1 (MQ=255)
                                                    tATGCGCGCCAACATTCGCGTGGCTGTGCAGTACATCGAGGCGTGGATCTCTGGCAACGGCTGTGTGCCGATTTATGGCCTGATGGAAGATgc                                      <  1:5271551/93‑1 (MQ=255)
                                                     aTGCGCGCCAACATTCGCGTGGCTGTGCAGTACATCGAGGCGTGGATCTCTGGCAACGGCTGTGTGCCGATTTATGGCCTGATGGAAGATgcg                                     >  2:7114477/1‑93 (MQ=255)
                                                     aTGCGCGCCAACATTCGCGTGGCTGTGCAGTACATCGAGGCGTGGATCTCTGGCAACGGCTGTGTGCCGATTTATGGCCTGATGGAAGATgag                                     >  1:6289382/1‑91 (MQ=255)
                                                     aTGCGCGCCAACATGCGGGTGGCTGTGCAGTACATCGAGGCGTGGATCTCTGGCAACGGCTGTGTGCCGATTTATGGCCTGATGGAAGATgcg                                     <  1:4084384/93‑1 (MQ=255)
                                                      tGCGCGCCAACATTCGCGTGGCTGTGCAGTACATCGAGGCGTGGATCTCTGGCAACGGCTGTGTGCCGATTTATGGCCTGATGGAAGATgcgg                                    >  1:6486545/1‑93 (MQ=255)
                                                         gcgcCAACATTCGCGTGGCTGTGCAGTACATCGAGGCGTGGATCTCTGGCAACGGCTGTGTGCCGATTTATGGCCTGATGGAAGATGCGGCGa                                 <  1:6777978/93‑1 (MQ=255)
                                                          cgcCAGCATTCCCGTGGCTGTGCAGTACAGCGAGGCGTGGATCTCTGGCAACGGCTGTGTGCCGATTTATGGCCTGATGGAAGATGCGGCGAc                                <  1:7364713/93‑1 (MQ=255)
                                                          cgcCAACATTCGCGTGGCTGTGCAGTACATCGAGGCGTGGATCTCTGGCAACGGCTGTGTGCCGATTTATGGCCTGATGGAAGATGCGGCGAc                                <  1:157472/93‑1 (MQ=255)
                                                           gcCAACATTCGCGTGGCTGTGCAGTACATCGAGGCGTGGATCTCTGGCAACGGCTGTGTGCCGATTTATGGCCTGATGGAAGATGCGGCGACg                               >  1:2461115/1‑93 (MQ=255)
                                                           gcCAACATTCGCGTGGCTGTGCAGTACATCGAGGCGTGGATCTCTGGCAACGGCTGTGTGCCGATTTATGGCCTGATGGAAGATGCGGCGACg                               <  1:3188546/93‑1 (MQ=255)
                                                            ccAACATTCGCGTGGCTGTGCAGTACATCGAGGCGTGGATCTCTGGCAACGGCTGTGTGCCGATTTATGGCCTGATGGAAGATGCGGGGACgg                              >  2:5908872/1‑93 (MQ=255)
                                                            ccAACATTCGCGTGGCTGTGCAGTACATCGAGGCGTGGATCTCTGGCAACGGCTGTGTGCCGATTTATGGCCTGATGGAAGATGCGGCGACgg                              >  2:4005037/1‑93 (MQ=255)
                                                            ccAACATTCGCGTGGCTGTGCAGTACATCGAGGCGTGGATCTCTGGCAACGGCTGTGTGCCGATTTATGGCCTGATGGAAGATGCGGCGACgg                              <  2:6504640/93‑1 (MQ=255)
                                                             cAACATTCGCGTGGCTGTGCAGTACATCGGGGCGTGGATCTCTGGCAACGGCTGTGTGCCGATTTATGGCCTGATGGAAGATGCGGCGACGGc                             >  2:4774951/1‑93 (MQ=255)
                                                              aaCATTCGCGTGGCTGTGCAGTACATCGAGGCGTGGATCTCTGGCAACGGCTGTGTGCCGATTTATGGCCTGATGGAAGATGCGGCGACGGCt                            >  2:2527805/1‑93 (MQ=255)
                                                              aaCATTCGCGTGGCTGTGCAGTACATCGAGGCGTGGATCTCTGGCAACGGCTGTGTGCCGATTTATGGCCTGATGGAAGATGCGGCGACGGCt                            <  2:1601555/93‑1 (MQ=255)
                                                              aaCATTCGCGTGGCTGTGCAGTACATCGAGGCGTGGATCTCTGGCAACGGCTGTGTGCCGATTTATGGCCTGATGGAAGATGCGGCGACGGCt                            >  1:3467769/1‑93 (MQ=255)
                                                               aCATTCGCGTGGCTGTGCAGTACATCGAGGCGTGGATCTCTGGCAACGGCTGTGTGCCGATTTATGGCCTGATGGAAGATGCGGCGACGGCTg                           <  1:2836978/93‑1 (MQ=255)
                                                                  ttCGCGTGGCTGTGCAGTACATCGAGGCGTGGATCTCTGGCAACGGCTGTGTGCCGATTTATGGCCTGATGGAAGATGCGGCGACGGCTGaaa                        >  2:6375662/1‑93 (MQ=255)
                                                                   tCGCGTGGCTGTGCAGTACATCGAGGCGTGGATCTCTGGCAACGGCTGTGTGCCGATTTATGGCCTGATGGAAGATGCGGCGACGGCTGAAAt                       <  2:2837372/93‑1 (MQ=255)
                                                                     gcgTGGCTGTGCAGTACATCGAGGCGTGGATCTCTGGCAACGGCTGTGTGCCGATTTATGGCCTGATGGAAGATGCGGCGACGGCTGAAAttt                     >  1:7202129/1‑93 (MQ=255)
                                                                       gTGGCTGTGCAGTACATCGAGGCGTGGATCTCTGGCAACGGCTGTGTGCCGATTTATGGCCTGATGGAAGATGCGGCGACGGCTGAAATTTcc                   >  2:181061/1‑93 (MQ=255)
                                                                        tGGCTGTGCAGTACATCGAGGCGTGGATCTCTGGCAACGGCTGTGTGCCGATTTATGGCCTGATGGAAGATGCGGCGACGGCTGAAATTTccc                  >  2:7487034/1‑93 (MQ=255)
                                                                          gCTGTGCAGTACATCGAGGCGTGGATCTCTGGCAACGGCTGTGTGCCGATTTATGGCCTGATGGAAGATGCGGCGACGGCTGAAATTTCCCGt                >  1:2337697/1‑93 (MQ=255)
                                                                          gCTGTGCAGTACATCGAGGCGTGGATCTCTGGCAACGGCTGTGTGCCGATTTATGGCCTGATGGAAGATGCGGCGACGGCTGAAATTTCCCGt                >  1:2095928/1‑93 (MQ=255)
                                                                           cTGTGCAGTACATCGAGGCGTGGACCTCTGGCAACGGCTGTGTGCCGATTTATGGCCTGATGGAAGATGCGGCGACGGCTGACATTTCCCGTa               <  2:2472464/93‑1 (MQ=255)
                                                                            tgtgCAGTACATCGAGGCGTGGATCTCTGGCAACGGCTGTGTGCCGATTTATGGCCTGATGGAAGATGCGGCGACGGCTGAAATTTCCCGTAc              >  2:849298/1‑93 (MQ=255)
                                                                            tgtgCAGTACATCGAGGCGTGGATCTCTGGCAACGGCTGTGTGCCGATTTATGGCCTGATGGAAGATGCGGCGACGGCTGAAATTTCCCGTAc              >  2:5352613/1‑93 (MQ=255)
                                                                            tgtgCAGTACATCGAGGCGTGGATCTCTGGCAACGGCTGTGTGCCGATTTATGGCCTGATGGAAGATGCGGCGACGGCTGAAATTTCCCGTAc              >  2:436417/1‑93 (MQ=255)
                                                                             gtgCAGTACATCGAGGCGTGGATCTCTGGCAACGGCTGTGTGCCGATTTATGGCCTGATGGAAGATGCGGCGACGGCTGAAATTTCCCGTAcc             <  1:4958689/93‑1 (MQ=255)
                                                                              tgCAGTACATCGAGGCGTGGATCTCTGGCAACGGCTGTGTGCCGATTTATGGCCTGATGGAAGATGGGGCGACGGCTGAAATTTCCCGTACCt            >  1:6143924/1‑93 (MQ=255)
                                                                              tgCAGTACATCGAGGCGTGGATCTCTGGCAACGGCTGTGTGCCGATTTATGGCCTGATGGAAGATGCGGCGACGGCTGAAATTTCCCGTACCt            <  2:3509282/93‑1 (MQ=255)
                                                                               gCAGTACATCGAGGCGTGGATCTCTGGCAACGGCTGTGTGCCGATTTATGGCCTGATGGAAGATGCGGCGACGGCTGAAATTTCCCGTACCTc           <  2:6179568/93‑1 (MQ=255)
                                                                               gCAGTACATCGAGGCGTGGATCTCTGGCAACGGCTGTGTGCCGATTTATGGCCTGATGGAAGATGCGGCGACGGCTGAAATTTCCCGTACCTc           >  2:3328803/1‑93 (MQ=255)
                                                                               gCAGTACATCGAGGCGTGGATCTCTGGCAACGGCTGTGTGCCGATTTATGGCCTGATGGAAGATGCGGCGACGGCTGAAATTTCCCGTACCTc           <  2:7620240/93‑1 (MQ=255)
                                                                                 aGTACATCGAGGCGTGGATCTCTGGCAACGGCTGTGTGCCGATTTATGGCCTGATGGAAGATGCGGCGACGGCTGAAATTTCCCGTACCTCGa         >  1:87419/1‑93 (MQ=255)
                                                                                    aCATCGAGGCGTGGATCTCTGGCAACGGCTGTGTGCCGATTTATGGCCTGATGGAAGATGCGGCGACGGCTGAAATTTCCCGTACCTCGATCt      <  2:3573715/93‑1 (MQ=255)
                                                                                      aTCGAGGCGTGGATCTCTGGCAACGGCTGTGTGCCGATTTATGGCCTGATGGAAGATGCGGCGACGGCTGAAATTTCCCGTACCTCGATCTgg    <  2:70105/93‑1 (MQ=255)
                                                                                       tCGAGGCGTGGATCTCTGGCAACGGCTGTGTGCCGATTTATGGCCTGATGGAAGATGCGGCGACGGCTGAAATTTCCCGTACCTCGATCTTGc   >  1:1173759/1‑93 (MQ=255)
                                                                                       tCGAGGCGTGGATCTCTGGCAACGGCTGTGTGCCGATTTATGGCCTGATGGAAGATGCGGCGACGGCTGAAATTTCCCGTACCTCGATCTGGc   >  2:4968006/1‑93 (MQ=255)
                                                                                       tCGAGGCGTGGATCTCTGGCAACGGCTGTGTGCCGATTTATGGCCTGATGGAAGATGCGGCGACGGCTGAAATTTCCCGTACCTCGATCTGGc   >  2:1927600/1‑93 (MQ=255)
                                                                                       tCGAGGCGTGGATCTCTGGCAACGGCTGTGTGCCGATTTATGGCCTGATGGAAGATGCGGCGACGGCTGAAATTTCCCGTACCTCGATCTGGc   >  1:7776913/1‑93 (MQ=255)
                                                                                       tCGAGGCGTGGATCTCTGGCAACGGCTGTGTGCCGATTTATGGCCTGATGGAAGATGCGGCGACGGCTGAAATTTCCCGTACCTCGATCTGGc   <  1:4684180/93‑1 (MQ=255)
                                                                                           |                                                                                         
TTACTGCCGATCAGCTGCTGGCACCTTGTGATGGTGAACGCACCGAAGAAGGTATGCGCGCCAACATTCGCGTGGCTGTGCAGTACATCGAAGCGTGGATCTCTGGCAACGGCTGTGTGCCGATTTATGGCCTGATGGAAGATGCGGCGACGGCTGAAATTTCCCGTACCTCGATCTGGCA  >  NC_000913/4214255‑4214435

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output Only 200 of 226 total aligned reads displayed.
GATTACTGCCGATCAGCTGCTGGCACCTTGTGATGGTGAACGCACCGAAGAAGGTATGCGCGCCAACATTCGCGTGGCTGTGCAGTACATCGAAGCGTGGATCTCTGGCAACGGCTGTGTGCCGATTTATGGCCTGATGGAAGATGCGGCGACGGCTGAAATTTCCCGTACCTCGATCTGGCAGTGG  >  NC_000913/4214253‑4214439
                                                                                             |                                                                                             
GATTACTGCCGATCAGCTGCTGGCACCTTGTGATGGTGAACGCACCGAAGAAGGTATGCGCGCCAACATTCGCGTGGCTGTGCAGTACATCGAG                                                                                               <  SRR4302543.904369/94‑1 (MQ=60)
GATTACTGCCGATCAGCTGCTGGCACCTTGTGATGGTGAACGCACCGAAGAAGGTATGCGCGCCAACATTCGCGTGGCTGTGCAGTACATCGAG                                                                                               >  SRR4302543.217588/1‑94 (MQ=60)
GATTACTGCCGATCAGCTGCTGGCACCTTGTGATGGTGAACGCACCGAAGAAGGTATGCGCGCCAACATTCGCGTGGCTGTGCAGTACATCGAG                                                                                               <  SRR4302543.6402262/94‑1 (MQ=60)
GATTACTGCCGATCAGCTGCTGGCACCTTGTGATGGTGAACGCACCGAAGAAGGTATGCGCGCCAACATTCGCGTGGCTGTGCAGTACATCGAG                                                                                               <  SRR4302543.2990079/94‑1 (MQ=60)
GATTACTGCCGATCAGCTGCTGGCACCTTGTGATGGTGAACGCACCGAAGAAGGTATGCGCGCCAACATTCGCGTGGCTGTGCAGTACATCGAG                                                                                               <  SRR4302543.3615206/94‑1 (MQ=60)
 ATTACTGCCGATCAGCTGCTGGCACCTTGTGATGGTGAACGCACCGAAGAAGGTATGCGCGCCAACATTCGCGTGGCTGTGCAGTACATCGAGG                                                                                              >  SRR4302543.366393/1‑94 (MQ=60)
 ATTACTGCCGATCAGCTGCTGGCACCTTGTGATGGTGAACGCACCGAAGAAGGTATGCGCGCCAACATTCGCGTGGCTGTGCAGTACATCGAGG                                                                                              >  SRR4302543.7952010/1‑94 (MQ=60)
 ATTACTGCCGATCAGCTGCTGGCACCTTGTGATGGTGAACGCACCGAAGAAGGTATGCGCGCCAACATTCGCGTGGCTGTGCAGTACATCGAGG                                                                                              <  SRR4302543.2878302/94‑1 (MQ=60)
 ATTACTGCCGATCAGCTGCTGGCACCTTGTGATGGTGAACGCACCGAAGAAGGTATGCGCGCCAACATTCGCGTGGCTGTGCAGTACATCGAGG                                                                                              <  SRR4302543.575073/94‑1 (MQ=60)
 ATTACTGCCGATCAGCTGCTGGCACCTTGTGATGGTGAACGCACCGAAGAAGGTATGCGCGCCAACATTCGCGTGGCTGTGCAGTACATCGAAG                                                                                              >  SRR4302543.7724357/1‑94 (MQ=60)
   TACTGCCGATCAGCTGCTGGCACCTTGTGATGGTGAACGCACCGAAGAAGGTATGCGCGCCAACATTCGCGTGGCTGTGCAGTACATCGAGGCG                                                                                            >  SRR4302543.1292814/1‑94 (MQ=60)
   TACTGCCGATCAGCTGCTGGCACCTTGTGATGGTGAACGCACCGAAGAAGGTATGCGCGCCAACATTCGCGTGGCTGTGCAGTACATCGAGGCG                                                                                            >  SRR4302543.6640004/1‑94 (MQ=60)
   TACTGCCGATCAGCTGCTGGCACCTTGTGATGGTGAACGCACCGAAGAAGGTATGCGCGCCAACATTCGCGTGGCTGTGCAGTACATCGAGGCG                                                                                            >  SRR4302543.1604804/1‑94 (MQ=60)
      TGCCGATCAGCTGCTGGCACCTTGTGATGGTGAACGCACCGAAGAAGGTATGCGCGCCAACATTCGCGTGGCTGTGCAGTACATCGAGGCGTGG                                                                                         <  SRR4302543.4159030/94‑1 (MQ=60)
       GCCGATCAGCTGCTGGCACCTTGTGATGGTGAACGCACCGAAGAAGGTATGCGCGCCAACATTCGCGTGGCTGTGCAGTACATCGAGGCGTGGA                                                                                        >  SRR4302543.5104204/1‑94 (MQ=60)
        CCGATCAGCTGCTGGCACCTTGTGATGGTGAACGCACCGAAGTAGGTATGCGCGCCAACATTCGCGTGGCTGTGCAGTATATCGAGGCGTGGAT                                                                                       >  SRR4302543.6232983/1‑94 (MQ=60)
        CCGATCAGCTGCTGGCACCTTGTGATGGTGAACGCACCGAAGAAGGTATGCGCGCCAACATTCGCGTGGCTGTGCAGTACATCGAGGCGTGGAT                                                                                       >  SRR4302543.503892/1‑94 (MQ=60)
         CGATCAGCTGCTGGCACCTTGTGATGGTGAACGCACCGAAGAAGGTATGCGCGCCAACATTCGCGTGGCTGTGCAGTACATCGAGGCGTGGATC                                                                                      >  SRR4302543.4468949/1‑94 (MQ=60)
         CGATCAGCTGCTGGCACCTTGTGATGGTGAACGCACCGAAGAAGGTATGCGCGCCAACATTCGCGTGGCTGTGCAGTACATCGAGGCGTGGATC                                                                                      >  SRR4302543.5971619/1‑94 (MQ=60)
         CGATCAGCTGCTGGCACCTTGTGATGGTGAACGCACCGAAGAAGGTATGCGCGCCAACATTCGCGTGGCTGTGCAGTACATCGAGGCGTGGATC                                                                                      >  SRR4302543.2027561/1‑94 (MQ=60)
         CGATCAGCTGCTGGCACCTTGTGATGGTGAACGCACCGAAGAAGGTATGCGCGCCAACATTCGCGTGGCTGTGCAGTACATCGAGGCGTGGATC                                                                                      >  SRR4302543.7786618/1‑94 (MQ=60)
          GATCAGCTGCTGGCACCTTGTGATGGTGAACGCACCGAAGAAGGTATGCGCGCCAACATTCGCGTGGCTGTGCAGTACATCGAGGCGTGGATCT                                                                                     >  SRR4302543.4650655/1‑94 (MQ=60)
          GATCAGCTGCTGGCACCTTGTGATGGTGAACGCACCGAAGAAGGTATGCGCGCCAACATTCGCGTGGCTGTGCAGTACATCGAGGCGTGGATCT                                                                                     >  SRR4302543.6713794/1‑94 (MQ=60)
          GATCAGCTGCTGGCACCTTGTGATGGTGAACGCACCGAAGAAGGTATGCGCGCCAACATTCGCGTGGCTGTGCAGTACATCGAGGCGTGGATCT                                                                                     >  SRR4302543.2720146/1‑94 (MQ=60)
          GATCAGCTGCTGGCACCTTGTGATGGTGAACGCACCGAAGAAGGTATGCGCGCCAACATTCGCGTGGCTGTGCAGTACATCGAGGCGTGGATCT                                                                                     >  SRR4302543.1994130/1‑94 (MQ=60)
           ATCAGCTGCTGGCACCTTGTGATGGTGAACGCACCGAAGAAGGTATGCGCGCCAACATTCGCGTGGCTGTGCAGTACATCGAGGCGTGGATCTC                                                                                    >  SRR4302543.4485703/1‑94 (MQ=60)
            TCAGCTGCTGGCACCTTGTGATGGTGAACGCACCGAAGAAGGTATGCGCGCCAACATTCGCGTGGCTGTGCAGTACATCGAGGCGTGGATCTCT                                                                                   <  SRR4302543.5723908/94‑1 (MQ=60)
            TCAGCTGCTGGCACCTTGTGATGGTGAACGCACCGAAGAAGGTATGCGCGCCAACATTCGCGTGGCTGTGCAGTACATCGAGGCGTGGATCTCT                                                                                   <  SRR4302543.1752876/94‑1 (MQ=60)
            TCAGCTGCTGGCACCTTGTGATGGTGAACGCACCGAAGAAGGTATGCGCGCCAACATTCGCGTGGCTGTGCAGTACATCGAGGCGTGGATCTCT                                                                                   <  SRR4302543.402925/94‑1 (MQ=60)
            TCAGCTGCTGGCACCTTGTGATGGTGAACGCACCGAAGAAGGTATGCGCGCCAACATTCGCGTGGCTGTGCAGTACATCGAGGCGTGGATCTCT                                                                                   <  SRR4302543.1911280/94‑1 (MQ=60)
             CAGCTGCTGGCACCTTGTGATGGTGAACGCACCGAAGAAGGTATGCGCGCCAACATTCGCGTGGCTGTGCAGTACATCGAGGCGTGGATCTCTG                                                                                  <  SRR4302543.7407598/94‑1 (MQ=60)
             CAGCTGCTGGCACCTTGTGATGGTGAACGCACCGAAGAAGGTATGCGCGCCAACATTCGCGTGGCTGTGCAGTACATCGAGGCGTGGATCTCTG                                                                                  <  SRR4302543.7947885/94‑1 (MQ=60)
              AGCTGCTGGCACCTTGTGATGGTGAACGCACCGAAGAAGGTATGCGCGCCAACATTCGCGTGGCTGTGCAGTACATCGAGGCGTGGATCTCTGG                                                                                 <  SRR4302543.509169/94‑1 (MQ=60)
               GCTGCTGGCACCTTGTGATGGTGAACGCACCGAAGAAGGTATGCGCGCCAACATTCGCGTGGCTGTGCAGTACATCGAGGCGTGGATCTCTGGC                                                                                <  SRR4302543.5056225/94‑1 (MQ=60)
               GCTGCTGGCACCTTGTGATGGTGAACGCACCGAAGAAGGTATGCGCGCCAACATTCGCGTGGCTGTGCAGTACATCGAGGCGTGGATCTCTGGC                                                                                <  SRR4302543.3665261/94‑1 (MQ=60)
                CTGCTGGCACCTTGTGATGGTGAACGCACCGAAGAAGGTATGCGCGCCAACATTCGCGTGGCTGTGCAGTACATCGAGGCGTGGATCTCTGGCA                                                                               >  SRR4302543.4903853/1‑94 (MQ=60)
                CTGCTGGCACCTTGTGATGGTGAACGCACCGAAGAAGGTATGCGCGCCAACATTCGCGTGGCTGTGCAGTACATCGAGGCGTGGATCTCTGCCA                                                                               >  SRR4302543.1216405/1‑94 (MQ=60)
                  GCTGGCACCTTGTGATGGTGAACGCACCGAAGAAGGTATGCGCGCCAACATTCGCGTGGCTGTGCAGTACATCGAGGCGTGGATCTCTGGCAAC                                                                             <  SRR4302543.6414943/94‑1 (MQ=60)
                  GCTGGCACCTTGTGATGGTGAACGCACCGAAGAAGGTATGCGCGCCAACATTCGCGTGGCTGTGCAGTACATCGAGGCGTGGATCTCTGGCAAC                                                                             <  SRR4302543.4823148/94‑1 (MQ=60)
                   CTGGCACCTTGTGATGGTGAACGCACCGAAGAAGGTATGCGCGCCAACATTCGCGTGGCTGTGCAGTACATCGAGGCGTGGATCTCTGGCAACG                                                                            >  SRR4302543.5733724/1‑94 (MQ=60)
                   CTGGCACCTTGTGATGGTGAACGCACCGAAGAAGGTATGCGCGCCAACATTCGCGTGGCTGTGCAGTACATCGAGGCGTGGATCTCTGGCAACG                                                                            <  SRR4302543.6852349/94‑1 (MQ=60)
                   CTGGCACCTTGTGATGGTGAACGCACCGAAGAAGGTATGCGCGCCAACATTCGCGTGGCTGTGCAGTACATAGAGGCGTGGATCTCTGGCAACG                                                                            <  SRR4302543.1415390/94‑1 (MQ=60)
                    TGGCACCTTGTGATGGTGAACGCACCGAAGAAGGTATGCGCGCCAACATTCGCGTGGCTGTGCAGTACATCGAGGCGTGGATCTCTGGCAACGG                                                                           >  SRR4302543.7036788/1‑94 (MQ=60)
                    TGGCACCTTGTGATGGTGAACGCACCGAAGAAGGTATGCGCGCCAACATTCGCGTGGCTGTGCAGTACATCGAGGCGTGGATCTCTGGCAACGG                                                                           >  SRR4302543.6742600/1‑94 (MQ=60)
                    TGGCACCTTGTGATGGTGAACGCACCGAAGAAGGTATGCGCGCCAACATTCGCGTGGCTGTGCAGTACATCGAGGCGTGGATCTCTGGCAACGG                                                                           >  SRR4302543.1914509/1‑94 (MQ=60)
                    TGGCACCTTGTGATGGTGAACGCACCGAAGAAGGTATGCGCGCCAACATTCGCGTGGCTGTGCAGTACATCGAGGCGTGGATCTCTGGCAACGG                                                                           >  SRR4302543.7859748/1‑94 (MQ=60)
                     GGCACCTTGTGATGGTGAACGCACCGAAGAAGGTATGCGCGCCAACATTCGCGTGGCTGTGCAGTACATCGAGGCGTGGATCTCTGGCAACGGC                                                                          <  SRR4302543.7536090/94‑1 (MQ=60)
                      GCACCTTGTGATGGTGAACGCACCGAAGAAGGTATGCGCGCCAACATTCGCGTGGCTGTGCAGTACATCGAGGCGTGGATCTCTGGCAACGGCT                                                                         <  SRR4302543.7967615/94‑1 (MQ=60)
                       CACCTTGTGATGGTGAACGCACCGAAGAAGGTATGCGCGCCAACATTCGCGTGGCTGTGCAGTACATCGAGGCGTGGATCTCTGGCAACGGCTG                                                                        >  SRR4302543.3363346/1‑94 (MQ=60)
                       CACCTTGTGATGGTGAACGCACCGAAGAAGGTATGCGCGCCAACATTCGCGTGGCTGTGCAGTACATCGAGGCGTGGATCTCTGGCAACGGCTG                                                                        <  SRR4302543.4656343/94‑1 (MQ=60)
                       CACCTTGTGATGGTGAACGCACCGAAGAAGGTATGCGCGCCAACATTCGCGTGGCTGTGCAGTACATCGAGGCGTGGATCTCTGGCAACGGCTG                                                                        >  SRR4302543.5563439/1‑94 (MQ=60)
                        ACCTTGTGATGGTGAACGCACCGAAGAAGGTATGCGCGCCAACATTCGCGTGGCTGTGCAGTACATCGAGGCGTGGATCTCTGGCAACGGCTGT                                                                       >  SRR4302543.4062942/1‑94 (MQ=60)
                         CCTTGTGATGGTGAACGCACCGAAGAAGGTATGCGCGCCAACATTCGCGTGGCTGTGCAGTACATCGAGGCGTGGATCTCTGGCAACGGCTGTG                                                                      >  SRR4302543.4059272/1‑94 (MQ=60)
                          CTTGTGATGGTGAACGCACCGAAGAAGGTATGCGCGCCAACATTCGCGTGGCTGTGCAGTACATCGAGGCGTGTATCTCTGGCAACGGCTGTGT                                                                     <  SRR4302543.1452176/94‑1 (MQ=60)
                          CTTGTGATGGTGAACGCACCGAAGAAGGTATGCGCGCCAACATTCGCGTGGCTGTGCAGTACATCGAGGCGTGGATCTCTGGCAACGGCTGTGT                                                                     <  SRR4302543.4864374/94‑1 (MQ=60)
                          CTTGTGATGGTGAACGCACCGAAGAAGGTATGCGCGCCAACATTCGCGTGGCTGTGCAGTACATCGAGGCGTGGATCTCTGGCAACGGCTGTGT                                                                     >  SRR4302543.3702757/1‑94 (MQ=60)
                          CTTGTGATGGTGAACGCACCGAAGAAGGTATGCGCGCCAACATTCGCGTGGCTGTGCAGTACATCGAGGCGTGGATCTCTGGCAACGGCTGTGG                                                                     >  SRR4302543.397652/1‑94 (MQ=60)
                            TGTGATGGTGAACGCACCGAAGAAGGTATGCGCGCCAACATTCGCGTGGCTGTGCAGTACATCGAGGCGTGGATCTCTGGCAACGGCTGTGTGC                                                                   >  SRR4302543.1295685/1‑94 (MQ=60)
                            TGTGATGGTGAACGCACCGAAGAAGGTATGCGCGCCAACATTCGCGTGGCTGTGCAGTACATCGAGGCGTGGATCTCTGGCAACGGCTGTGTGC                                                                   <  SRR4302543.3620489/94‑1 (MQ=60)
                            TGTGATGGTGAACGCACCGAAGAAGGTATGCGCGCCAACATTCGCGTGGCTGTGCAGTACATCGAGGCGTGGATCTCTGGCAACGGCTGTGTGC                                                                   >  SRR4302543.2175491/1‑94 (MQ=60)
                             GTGATGGTGAACGCACCGAAGAAGGTATGCGCGCCAACATTCGCGTGGCTGTGCAGTACATCGAGGCGTGGATCTCTGGCAACGGCTGTGTGCC                                                                  <  SRR4302543.5363003/94‑1 (MQ=60)
                             GTGATGGTGAACGCACCGAAGAAGGTATGCGCGCCAACATTCGCGTGGCTGTGCAGTACATCGAGGCGTGGATCTCTGGCAACGGCTGTGTGCC                                                                  >  SRR4302543.3970073/1‑94 (MQ=60)
                             GTGATGGTGAACGCACCGAAGAAGGTATGCGCGCCAACATTCGCGTGGCTGTGCAGTACATCGAGGCGTGGATCTCTGGCAACGGCTGTGTGCC                                                                  <  SRR4302543.5748484/94‑1 (MQ=60)
                             GTGATGGTGAACGCACCGAAGAAGGTATGCGCGCCAACATTCGCGTGGCTGTGCAGTACATCGAGGCGTGGATCTCTGGCAACGGCTGTGTGCC                                                                  <  SRR4302543.5534827/94‑1 (MQ=60)
                              TGATGGTGAACGCACCGAAGAAGGTATGCGCGCCAACATTCGCGTGGCTGTGCAGTACATCGAGGCGTGGATCTCTGGCAACGGCTGTGTGCCG                                                                 <  SRR4302543.266451/94‑1 (MQ=60)
                               GATGGTGAACGCACCGAAGAAGGTATGCGCGCCAACATTCGCGTGGCTGTGCAGTACATCGAGGCGTGGATCTCTGGCAACGGCTGTGTGCCGT                                                                >  SRR4302543.4593940/1‑94 (MQ=60)
                               GATGGTGAACGCACCGAAGAAGGTATGCGCGCCAACATTCGCGTGGCTGTGCAGTACATCGAGGCGTGGATCTCTGGCAACGGCTGTGTGCCGA                                                                >  SRR4302543.4207613/1‑94 (MQ=60)
                               GATGGTGAACGCACCGAAGAAGGTATGCGCGCCAACATTCGCGTGGCTGTGCAGTACATCGAGGCGTGGATCTCTGGCAACGGCTGTGTGCCGA                                                                >  SRR4302543.6644471/1‑94 (MQ=60)
                               GATGGTGAACGCACCGAAGAAGGTATGCGCGCCAACATTCGCGTGGCTGTGCAGTACATCGAGGCGTGGATCTCTGGCAACGGCTGTGTGCCGA                                                                >  SRR4302543.724195/1‑94 (MQ=60)
                               GATGGTGAACGCACCGAAGAAGGTATGCGCGCCAACATTCGCGTGGCTGTGCAGTACATCGAGGCGTGGATCTCTGGCAACGGCTGTGTGCCGA                                                                <  SRR4302543.6745437/94‑1 (MQ=60)
                               GATGGTGAACGCACCGAAGAAGGTATGCGCGCCAACATTCGCGTGGCTGTGCAGTACATCGAGGCGTGGATCTCTGGCAACGGCTGTGTGCCGA                                                                >  SRR4302543.7176556/1‑94 (MQ=60)
                               GATGGTGAACGCACCGAAGAAGGTATGCGCGCCAACATTCGCGGGGCTGTGCAGTACATCGAGGCGTGGATCTCTGGCAACGGCTGAGTGCCGT                                                                >  SRR4302543.3293812/1‑94 (MQ=60)
                                ATGGTGAACGCACCGAAGAAGGTATGCGCGCCAACATTCGCGTGGCTGTGCAGTGCATCGAGGCGTGGATCTCTGGCAACGGCTGTGTGCCGAT                                                               <  SRR4302543.6922020/94‑1 (MQ=60)
                                ATGGTGAACGCACCGAAGAAGGTATGCGCGCCAACATTCGCGTGGCTGTGCAGTACATCGAGGCGTGGATCTCTGGCAACGGCTGTGTGCCGAT                                                               <  SRR4302543.4060000/94‑1 (MQ=60)
                                 TGGGGAACGCACCGAAGAAGGTATGCGCGCCAACATTCGCGTTGCTGTGCAGTACATCGAGGCGTGGATCTCTGGCAACGGCTGTGTGCCGATT                                                              <  SRR4302543.2082531/94‑1 (MQ=60)
                                  GGTGAACGCACCGAAGAAGGTATGCGCGCCAACTTTCGCGTGGCTGTGCAGTACATCGAGGCGTGGATCTCTGGCAACGGCTGTGTGCCGATTT                                                             <  SRR4302543.7678027/94‑1 (MQ=60)
                                  GGTGAACGCACCGAAGAAGGTATGCGCGCCAACATTCGCGTGGCTGTGCAGTACATCGAGGCGTGGATCTCTGGCAACGGCTGTGTGCCGATTT                                                             <  SRR4302543.1637931/94‑1 (MQ=60)
                                   GTGAACGCACCGAAGAAGGTATGCGCGCCAACATTCGCGTGGCTGTGCAGTACATCGAGGCGTGGATCTCTGGCAACGGCTGTGTGCCGATTTA                                                            >  SRR4302543.1320967/1‑94 (MQ=60)
                                   GTGAACGCACCGAAGAAGGTATGCGCGCCAACATTCGCGTGGCTGTGCAGTACATCGAGGCGTGGATCTCTGGCAACGGCTGTGTGCCGATTTA                                                            >  SRR4302543.5719228/1‑94 (MQ=60)
                                    TGAACGCACCGAAGAAGGTATGCGCGCCAACATTCGCGTGGCTGTGCAGTACATCGAGGCGTGGATCTCTGGCAACGGCTGTGTGCCGATTTAT                                                           <  SRR4302543.4619303/94‑1 (MQ=60)
                                    TGAACGCACCGAAGAAGGTATGCGCGCCAACATTCGCGTGGCTGTGCAGTACATCGAGGCGTGGATCTCTGGCAACGGCTGTGTGCCGATTTAT                                                           <  SRR4302543.4871232/94‑1 (MQ=60)
                                    TGAACGCACCGAAGAAGGTATGCGCGCCAACATTCGCGTGGCTGTGCAGTACATCGAGGCGTGGATCTCTGGCAACGGCTGTGTGCCGATTTAT                                                           <  SRR4302543.1040688/94‑1 (MQ=60)
                                    TGAACGCACCGAAGAAGGTATGCGCGCCAACATTCGCGTGGCTGTGCAGTACATCGAGGCGTGGATCTCTGGCAACGGCTGTGTGCCGATTTAT                                                           <  SRR4302543.759007/94‑1 (MQ=60)
                                    TGAACGCACCGAAGAAGGTATGCGCGCCAACATTCGCGTGGCTGTGCAGTACATCGAGGCGTGGATCTCTGGCAACGGCTGTGTGCCGATTTAT                                                           <  SRR4302543.6605450/94‑1 (MQ=60)
                                    TGAACGCACCGAAGAAGGTATGCGCGCCAACATTCGCGTGGCTGTGCAGTACATCGAGGCGTGGATCTCTGGCAACGGCTGTGTGCCGATTTAT                                                           <  SRR4302543.3061933/94‑1 (MQ=60)
                                    TGAACGCACCGAAGAAGGTATGCGCGCCAACATTCGCGTGGCTGTGCAGTACATCGAGGCGTGGATCTCTGGCAACGGCTGTGTGCCGATTTAT                                                           <  SRR4302543.3717014/94‑1 (MQ=60)
                                      AACGCACCGAAGAAGGTATGCGCGCCAACATTCGCGTGGCTGTGCAGTACATCGAGGCGTGGATCTCTGGCAACGGCTGTGTGCCGATTTATGG                                                         <  SRR4302543.4448832/94‑1 (MQ=60)
                                       ACGCACCGAAGAAGGTATGCGCGCCAACATTCGCGTGGCTGTGCAGTACATCGAGGCGTGGATCTCTGGCAACGGCTGTGTGCCGATTTATGGC                                                        <  SRR4302543.7745864/94‑1 (MQ=60)
                                        CGCACCGAAGAAGGTATGCGCGCCAACATTCGCGTGGCTTTGCAGTACATCGAGGCGTGGATCTCTGGCAACGGCTGTGTGCCGATTTATGGCC                                                       >  SRR4302543.5056706/1‑94 (MQ=60)
                                        CGCACCGAAGAAGGTATGCGCGCCAACATTCGCGTGGCTGTGCAGTACATCGAGGCGTGGATCTCTGGCAACGGCTGTGTGCCGATTTATGGCC                                                       <  SRR4302543.6060476/94‑1 (MQ=60)
                                        CGCACCGAAGAAGGTATGCGCGCCAACATTCGCGTGGCTGTGCAGTACATCGAGGCGTGGATCTCTGGCAACGGCTGTGTGCCGATTTATGGCC                                                       <  SRR4302543.259932/94‑1 (MQ=60)
                                           ACCGAAGAAGGTATGCGCGCCAACATTCGCGTGGCTGTGCAGTACATCGAGGCGTGGATCTCTGGCAACGGCTGTGTGCCGATTTATGGCCTGA                                                    >  SRR4302543.1111155/1‑94 (MQ=60)
                                           ACCGAAGAAGGTATGCGCGCCAACATTCGCGTGGCTGTGCAGTACATCGAGGCGTGGATCTCTGGCAACGGCTGTGTGCCGATTTATGGCCTGA                                                    >  SRR4302543.3979467/1‑94 (MQ=60)
                                           ACCGAAGAAGGTATGCGCGCCAACATTCGCGTGGCTGTGCAGTACATCGAGGCGTGGATCTCTGGCAACGGCTGTGTGCCGATTTATGGCCTGA                                                    >  SRR4302543.4278749/1‑94 (MQ=60)
                                           ACCGAAGAAGGTATGCGCGCCAACATTCGCGTGGCTGTGCAGTACATCGAGGCGTGGATCTCTGGCAACGGCTGTGTGCCGATTTATGGCCTGA                                                    >  SRR4302543.1548705/1‑94 (MQ=60)
                                           ACCGAAGAAGGTATGCGCGCCAACATTCGCGTGGCTGTGCAGTACATCGAGGCGTGGATCTCTGGCAACGGCTGTGTGCCGATTTATGGCCTGA                                                    <  SRR4302543.7548323/94‑1 (MQ=60)
                                             CGAAGAAGGTATGCGCGCCAACATTCGCGTGGCTGTGCAGTACATCGAGGCGTGGATCTCTGGCAACGGCTGTGTGCCGATTTATGGCCTGATG                                                  >  SRR4302543.1077054/1‑94 (MQ=60)
                                             CGAAGAAGGTATGCGCGCCAACATTCGCGTGGCTGTGCAGTACATCGAGGCGTGGATCTCTGGCAACGGCTGTGTGCCGATTTATGGCCTGATG                                                  <  SRR4302543.4579428/94‑1 (MQ=60)
                                             CGAAGAAGGTATGCGCGCCAACATTCGCGTGGCTGTGCAGTACATCGAGGCGTGGATCTCTGGCAACGGCTGTGTGCCGATTTAT‑GCCTGATTG                                                 >  SRR4302543.6922060/1‑94 (MQ=60)
                                              GAAGAAGGTATGCGCGCCAACATTCGCGTGGCTGTGCAGTACATCGAGGCGTGGATCTCTGGCAACGGCTGTGTGCCGATTTATGGCCTGATGG                                                 <  SRR4302543.7039683/94‑1 (MQ=60)
                                              GAAGAAGGTATGCGCGCCAACATTCGCGTGGCTGTGCAGTACATCGAGGCGTGGATCTCTGGCAACGGCTGTGTGCCGATTTATGGCCTGATGG                                                 <  SRR4302543.3584828/94‑1 (MQ=60)
                                              GAAGAAGGTATGCGCGCCAACATTCGCGTGGCTGTGCAGTACATCGAGGCGTGGATCTCTGGCAACGGCTGTGTGCCGATTTATGGCCTGATGG                                                 <  SRR4302543.5410070/94‑1 (MQ=60)
                                               AAGAAGGTATGCGCGCCAACATTCGCGTGGCTGTGCAGTACATCGAGGCGTGGATCTCTGGCAACGGCTGTGTGCCGATTTATGGCCTGATGGA                                                >  SRR4302543.3178977/1‑94 (MQ=60)
                                                AGAAGGTATGCGCGCCAACATTCGCGTGGCTGTGCAGTACATCGAGGCGTGGATCTCTGGCAACGGCTGTGTGCCGATTTATGGCCTGATGGAA                                               >  SRR4302543.1483488/1‑94 (MQ=60)
                                                AGAAGGTATGCGCGCCAACATTCGCGTGGCTGTGCAGTACATAGAGGCGTGGATCTCTGGCAACGGCTGTGTGCCGATTTATGGCCTGATGGAA                                               >  SRR4302543.659084/1‑94 (MQ=60)
                                                 GAAGGTATGCGCGCCAACATTCGCGTGGGTGTGGAGTACATAGAGGGGAGGATGTCTGGCAAAGGCTGTGTGACGATTTATGGCCTGAGGGAAG                                              >  SRR4302543.2911772/1‑94 (MQ=42)
                                                 GAAGGTATGCGCGCCAACATTCGCGTGGCTGTGCAGTACATCGAGGCGTGGATCTCTGGCAACGGCTGTGTGCCGATTTATGGCCTGATGGAAG                                              <  SRR4302543.2466499/94‑1 (MQ=60)
                                                 GAAGGTATGCGCGCCAACATTCGCGTGGCTGTGCAGTACATCGAGGCGTGGATCTCTGGCAACGGCTGTGTGCCGATTTATGGCCTGATGGAAG                                              <  SRR4302543.7008178/94‑1 (MQ=60)
                                                 GAAGGTATGCGCGCCAACATTCGCGTGGCTGTGCAGTACATCGAGGCGTGGATCTCTGGCAACGGCTGTGTGCCGATTTATGGCCTGATGGAAG                                              >  SRR4302543.3124438/1‑94 (MQ=60)
                                                 AAAGGTATGCGCGCCAACATTCGCGTGGCTGTGCAGTACATCGAGGCGTGGATCTCTGGCAACGGCTGTGTGCCGATTTATGGCCTGATGGAAG                                              <  SRR4302543.5173084/94‑1 (MQ=60)
                                                  AAGGTATGCGCGCCAACATTCGCGTGGCTGTGCAGTACATCGAGGCGTGGATCTCTGGCAACGGCTGTGTGCCGATTTATGGCCTGATGGAAGA                                             <  SRR4302543.375078/94‑1 (MQ=60)
                                                  AAGGTATGCGCGCCAACATTCGCGTGGCTGTGCAGTACATCGAGGCGTGGATCTCTGGCAACGGCTGTGTGCCGATTTATGGCCTGATGGAAGA                                             >  SRR4302543.7903176/1‑94 (MQ=60)
                                                  AAGGTATGCGCGCCAACATTCGCGTGGCTGTGCAGTACATCGAGGCGTGGATCTCTGGCAACGGCTGTGTGCCGATTTATGGCCTGATGGAAGA                                             <  SRR4302543.4268121/94‑1 (MQ=60)
                                                  AAGGTATGCGCGCCAACATTCGCGTGGCTGTGCAGTACATCGAGGCGTGGATCTCTGGCAACGGCTGTGTGCCGATTTATGGCCTGATGGAAGA                                             <  SRR4302543.4377030/94‑1 (MQ=60)
                                                   AGGTATGCGCGCCAACATTCGCGTGGCTGTGCAGTACATCGAGGCGTGGATCTCTGGCAACGGCTGTGTGCCGATTTATGGCCTGATGGAAGAT                                            <  SRR4302543.5796096/94‑1 (MQ=60)
                                                   AGGTATGCGCGCCAACATTCGCGTGGCTGTGCAGTACATCGAGGCGTGGATCTCTGGCAACGGCTGTGTGCCGATTTATGGCCTGATGGAAGAT                                            <  SRR4302543.2667671/94‑1 (MQ=60)
                                                   AGGTATGCGCGCCAACATTCGCGTGGCTGTGCAGTACATCGAGGCGTGGATCTCTGGCAACGGCTGTGTGCCGATTTATGGCCTGATGGAAGAT                                            <  SRR4302543.3195018/94‑1 (MQ=60)
                                                   AGGTATGCGCGCCAACATTCGCGTGGCTGTGCAGTACATCGAGGCGTGGATCTCTGGCAACGGCTGTGTGCCGATTTATGGCCTGATGGAAGAT                                            <  SRR4302543.5023860/94‑1 (MQ=60)
                                                   AGGTATGCGCGCCAACATTCGCGTGGCTGTGCAGTACATCGAGGCGTGGATCTCTGGCAACGGCTGTGTGCCGATTTATGGCCTGATGGAAGAT                                            <  SRR4302543.4932745/94‑1 (MQ=60)
                                                   AGGTATGCGCGCCAACATTCGCGTGGCTGTGCAGTACATCGAGGCGTGGATCTCTGGCAACGGCTGTGTGCCGATTTATGGCCTGATGGAAGAT                                            >  SRR4302543.718559/1‑94 (MQ=60)
                                                   AGGTATGCGCGCCAACATTCGCGTGGCTGTGCAGTACATCGAGGCGTGGATCTCTGGCAACGGCTGTGTGCCGATTTATGGCCTGATGGAAGAT                                            <  SRR4302543.7419019/94‑1 (MQ=60)
                                                   AGGTATGCGCGCCAACATTCGCGTGGCTGTGCAGTACATCGAGGCGTGGATCTCTGGCAACGGCTGTGTGCCGATTTATGGCCTGATGGAAGAT                                            <  SRR4302543.4706883/94‑1 (MQ=60)
                                                   AGGTATGCGCGCCAACATTCGCGTCGCTGTGCAGTACATCGAGGCGTGGATCTCTGGCAACGGCTGTGTGCCGATTTATGGCCTGATGGAAGAT                                            <  SRR4302543.7030120/94‑1 (MQ=60)
                                                    GGTATGCGCGCCAACATTCGCGTGGCTGTGCAGTACATCGAAGCGTGGATCTCTGGCAACGGCTGTGTGCCGATTTATGGCCTGATGGAAGATG                                           <  SRR4302543.3424923/94‑1 (MQ=60)
                                                      TATGCGCGCCAACATTCGCGTGGCTGTGCAGTACATCGAGGCGTGGATCTCTGGCAACGGCTGTGTGCCGATTTATGGCCTGATGGAAGATGCG                                         <  SRR4302543.5319107/94‑1 (MQ=60)
                                                      TATGCGCGCCAACATTCGCGTGGCTGTGCAGTACATCGAGGCGTGGATCTCTGGCAACGGCTGTGTGCCGATTTATGGCCTGATGGAAGATGCG                                         <  SRR4302543.158580/94‑1 (MQ=60)
                                                      TATGCGCGCCAACATTCGCGTGGCTGTGCAGTACATCGAGGCGTGGATCTCTGGCAACGGCTGTGTGCCGATTTATGGCCTGATGGAAGATGCG                                         <  SRR4302543.5899566/94‑1 (MQ=60)
                                                      TATGCGCGCCAACATTCGCGTGGCTGTGCAGTACATCGAGGCGTGGATCTCTGGCAACGGCTGTGTGCCGATTTATGGCCTGATGGAAGATGCG                                         <  SRR4302543.6252536/94‑1 (MQ=60)
                                                      TATGCGCGCCAACATTCGCGTGGCTGTGCAGTACATCGAGGCGTGGATCTCTGGCAACGGCTGTGTGCCGATTTATGGCCTGATGGAAGATGCG                                         <  SRR4302543.4193458/94‑1 (MQ=60)
                                                      TATGCGCGCCAACATTCGCGTGGCTGTGCAGTACATCGAGGCGTGGATCTCTGGCAACGGCTGTGTGCCGATTTATGGCCTGATGGAAGATGAG                                         >  SRR4302543.6348611/1‑94 (MQ=60)
                                                       ATGCGCGCCAACATTCGCGTGGCTGTGCAGTACATCGAGGCGTGGATCTCTGGCAACGGCTGTGTGCCGATTTATGGCCTGATGGAAGATGCGG                                        >  SRR4302543.5153915/1‑94 (MQ=60)
                                                       ATGCGCGCCAACATTCGCGTGGCTGTGCAGTACATCGAGGCGTGGATCTCTGGCAACGGCTGTGTGCCGATTTATGGCCTGATGGAAGATGCGG                                        >  SRR4302543.6547659/1‑94 (MQ=60)
                                                       ATGCGCGCCAACATTCGCGTGGCTGTGCAGTACATCGAGGCGTGGATCTCTGGCAACGGCTGTGTGCCGATTTATGGCCTGATGGAAGATGCGG                                        <  SRR4302543.6703330/94‑1 (MQ=60)
                                                       ATGCGCGCCAACATTCGCGTGGCTGTGCAGTACATCGAGGCGTGGATCTCTGGCAACGGCTGTGTGCCGATTTATGGCCTGATGGAAGATGCGG                                        <  SRR4302543.686228/94‑1 (MQ=60)
                                                        TGCGCGCCAACATTCGCGTGGCTGTGCAGTACATCGAGGCGTGGATCTCTGGCAACGGCTGTGTGCCGATTTATGGCCTGATGGAAGATGCGGC                                       <  SRR4302543.7524866/94‑1 (MQ=60)
                                                           GCGCCAACATTCGCGTGGCTGTGCAGTACATCGAGGCGTGGATCTCTGGCAACGGCTGTGTGCCGATTTATGGCCTGATGGAAGATGCGGCGAG                                    >  SRR4302543.885616/1‑94 (MQ=60)
                                                           GCGCCAACATTCGCGTGGCTGTGCAGTACATCGAGGCGTGGATCTCTGGCAACGGCTGTGTGCCGATTTATGGCCTGATGGAAGATGCGGCGAC                                    <  SRR4302543.6841996/94‑1 (MQ=60)
                                                           GCGCCAACATTCGCGTGGCTGTGCAGTACATCGAGGCGTGGATCTCTGGCAACGGCTGTGTGCCGATTTATGGCCTGATGGAAGATGCGGCGAC                                    >  SRR4302543.2312138/1‑94 (MQ=60)
                                                            CGCCAGCATTCCCGTGGCTGTGCAGTACAGCGAGGCGTGGATCTCTGGCAACGGCTGTGTGCCGATTTATGGCCTGATGGAAGATGCGGCGACG                                   <  SRR4302543.7435530/94‑1 (MQ=60)
                                                            CGCCAACATTCGCGTGGCTGTGCAGTACATCGAGGCGTGGATCTCTGGCAACGGCTGTGTGCCGATTTATGGCCTGATGGAAGATGCGGCGACG                                   <  SRR4302543.158825/94‑1 (MQ=60)
                                                            CGCCAACATTCGCGTGGCTGTGCAGTACATCGAGGCGTGGATCTCTGGCAACGGCTGTGTGCCGATTTATGGCCTGATGGAAGATGCGGCGACG                                   >  SRR4302543.2483303/1‑94 (MQ=60)
                                                            CGCCAACATTCGCGTGGCTGTGCAGTACATCGAGGCGTGGATCTCTGGCAACGGCTGTGTGCCGATTTATGGCCTGATGGAAGATGCGGCGACG                                   >  SRR4302543.6949746/1‑94 (MQ=60)
                                                            CGCCAACATTCGCGTGGCTGTGCAGTACATCGAGGCGTGGATCTCTGGCAACGGCTGTGTGCCGATTTATGGCCTGATGGAAGATGCGGCGACG                                   <  SRR4302543.3804552/94‑1 (MQ=60)
                                                             GCCAACATTCGCGTGGCTGTGCAGTACATCGAGGCGTGGATCTCTGGCAACGGCTGTGTGCCGATTTATGGCCTGATGGAAGATGCGGCGACGG                                  <  SRR4302543.3217301/94‑1 (MQ=60)
                                                              CCAACATTCGCGTGGCTGTGCAGTACATCGAGGCGTGGATCTCTGGCAACGGCTGTGTGCCGATTTATGGCCTGATGGAAGATGCGGCGACGGC                                 >  SRR4302543.3836070/1‑94 (MQ=60)
                                                              CCAACATTCGCGTGGCTGTGCAGTACATCGAGGCGTGGATCTCTGGCAACGGCTGTGTGCCGATTTATGGCCTGATGGAAGATGCGGCGACGGC                                 >  SRR4302543.2322572/1‑94 (MQ=60)
                                                               CAACATTCGCGTGGCTGTGCAGTACATCGAGGCGTGGATCTCTGGCAACGGCTGTGTGCCGATTTATGGCCTGATGGAAGATGCGGCGACGGCT                                >  SRR4302543.3498830/1‑94 (MQ=60)
                                                                AACATTCGCGTGGCTGTGCAGTACATCGAGGCGTGGATCTCTGGCAACGGCTGTGTGCCGATTTATGGCCTGATGGAAGATGCGGCGACGGCTG                               >  SRR4302543.600303/1‑94 (MQ=60)
                                                                 ACATTCGCGTGGCTGTGCAGTACATCGAGGCGTGGATCTCTGGCAACGGCTGTGTGCCGATTTATGGCCTGATGGAAGATGCGGCGACGGCTGA                              >  SRR4302543.3289182/1‑94 (MQ=60)
                                                                 ACATTCGCGTGGCTGTGCAGTACATCGAGGCGTGGATCTCTGGCAACGGCTGTGTGCCGATTTATGGCCTGATGGAAGATGCGGCGACGGCTGA                              <  SRR4302543.2862918/94‑1 (MQ=60)
                                                                 ACATTCGCGTGGCTGTGCAGTACATCGAGGCGTGGATCTCTGGCAACGGCTGTGTGCCGATTTATGGCCTGATGGAAGATGCGGCGACGGCTGA                              <  SRR4302543.3519187/94‑1 (MQ=60)
                                                                 ACATTCGCGTGGCTGTGCAGTACATCGAGGCGTGGATCTCTGGCAACGGCTGTGTGCCGATTTATGGCCTGATGGAAGATGCGGCGACGGCTGA                              >  SRR4302543.694855/1‑94 (MQ=60)
                                                                   ATTCGCGTGGCTGTGCGGTACATCGAGGCGTGGATCTCTGGCAACGGCTGTGTGCCGGTTTATGGCCTGATGGAAGATGCGGCGCCGGCTGAAG                            >  SRR4302543.6087188/1‑94 (MQ=60)
                                                                   ATTCGCGTGGCTGTGCAGTACATCGAGGCGTGGATCTCTGGCAACGGCTGTGTGCCGATTTATGGCCTGATGGAAGATGCGGCGACGGCTGAAA                            >  SRR4302543.7127050/1‑94 (MQ=60)
                                                                   ATTCGCGTGGCTGTGCAGTACATCGAGGCGTGGATCTCTGGCAACGGCTGTGTGCCGATTTATGGCCTGATGGAAGATGCGGCGACGGCTGAAA                            >  SRR4302543.1418162/1‑94 (MQ=60)
                                                                   ATTCGCGTGGCTGTGCAGTACATCGAGGCGTGGATCTCTGGCAACGGCTGTGTGCCGATTTATGGCCTGATGGAAGATGCGGCGACGGCTGAAA                            >  SRR4302543.1961248/1‑94 (MQ=60)
                                                                   ATTCGCGTGGCTGTGCAGTACATCGAGGCGTGGATCTCTGGCAACGGCTGTGTGCCGATTTATGGCCTGATGGAAGATGCGGCGACGGCTGAAA                            <  SRR4302543.1891651/94‑1 (MQ=60)
                                                                   ATTCGCGTGGCTGTGCAGTACATCGAGGCGTGGATCTCTGGCAACGGCTCTGTGCCGATTTATGGCCTGATGGAAGATGCGGCGACGGCTGAAA                            >  SRR4302543.2799511/1‑94 (MQ=60)
                                                                     TCGCGTGGCTGTGCAGTACATCGAGGCGTGGATCTCTGGCAACGGCTGTGTGCCGATTTATGGCCTGATGGAAGATGCGGCGACGGCTGAAATT                          <  SRR4302543.5723077/94‑1 (MQ=60)
                                                                      CGCGTGGCTGTGCAGTACATCGAGGCGTGGATCTCTGGCAACGGCTGTGTGCCGATTTATGGCCTGATGGAAGATGCGGCGACGGCTGAAATTT                         >  SRR4302543.7271954/1‑94 (MQ=60)
                                                                      CGCGTGGCTGTGCAGTACATCGAGGCGTGGATCTCTGGCAACGGCTGTGTGCCGATTTATGGCCTGATGGAAGATGCGGCGACGGCTGAAATTT                         <  SRR4302543.7670260/94‑1 (MQ=60)
                                                                       GCGTGGCTGTGCAGTACATCGAGGCGTGGATCTCTGGCAACGGCTGTGTGCCGATTTATGGCCTGATGGAAGATGCGGCGACGGCTGAAATTTC                        >  SRR4302543.123734/1‑94 (MQ=60)
                                                                        CGTGGCTGTGCAGTACATCGAGGCGTGGATCTCTGGCAACGGCTGTGTGCCGATTTATGGCCTGATGGAAGATGCGGCGACGGCTGAAATTTCC                       >  SRR4302543.4437838/1‑94 (MQ=60)
                                                                        CGTGGCTGTGCAGTACATCGAGGCGTGGATCTCTGGCAACGGCTGTGTGCCGATTTATGGCCTGATGGAAGATGCGGCGACGGCTGAAATTTCC                       >  SRR4302543.2897594/1‑94 (MQ=60)
                                                                         GTGGCTGTGCAGTACATCGAGGCGTGGATCTCTGGCAACGGCTGTGTGCCGATTTATGGCCTGATGGAAGATGCGGCGACGGCTGAAATTTCCC                      <  SRR4302543.826227/94‑1 (MQ=60)
                                                                         GGGGCTGTGCAGTACATCGAGGCGTGGATCTCTGGCAACGGCTGTGTGCCGATTTATGGCCTGATGGAAGATGCGGCGACGGCTGAAATTTCCC                      <  SRR4302543.6587412/94‑1 (MQ=60)
                                                                          TGGCTGTGCAGTACATCGAGGCGTGGATCTCTGGCAACGGCTGTGTGCCGATTTATGGCCTGATGGAAGATGCGGCGACGGCTGAAATTTCCCG                     >  SRR4302543.484063/1‑94 (MQ=60)
                                                                          TGGCTGTGCAGTACATCGAGGCGTGGATCTCTGGCAACGGCTGTGTGCCGATTTATGGCCTGATGGAAGATGCGGCGACGGCTGAAATTTCCCG                     <  SRR4302543.2400149/94‑1 (MQ=60)
                                                                          TGGCTGTGCAGTACATCGAGGCGTGGATCTCTGGCAACGGCTGTGTGCCGATTTATGGCCTGATGGAAGATGCGGCGACGGCTGAAATTTCCCG                     <  SRR4302543.651553/94‑1 (MQ=60)
                                                                          TGGCTGTGCAGTACATCGAGGCGTGGATCTCTGGCAACGGCTGTGTGCCGATTTATGGCCTGATGGAAGATGCGGCGACGGCTGAAATTTCCCG                     <  SRR4302543.7232936/94‑1 (MQ=60)
                                                                           GGCTGTGCAGTACATCGAGGCGTGGATCTCTGGCAACGGCTGTGTGCCGATTTATGGCCTGATGGAAGATGCGGCGACGGCTGAAATTTCCCGT                    >  SRR4302543.2114657/1‑94 (MQ=60)
                                                                           GGCTGTGCAGTACATCGAGGCGTGGATCTCTGGCAACGGCTGTGTGCCGATTTATGGCCTGATGGAAGATGCGGCGACGGCTGAAATTTCCCGT                    <  SRR4302543.3820286/94‑1 (MQ=60)
                                                                             CTGTGCAGTACATCGAGGCGTGGATCTCTGGCAACGGCTGTGTGCCGATTTATGGCCTGATGGAAGATGCGGCGACGGCTGAAATTTCCCGTAC                  <  SRR4302543.2825645/94‑1 (MQ=60)
                                                                             CTGTGCAGTACATCGAGGCGTGGATCTCTGGCAACGGCTGTGTGCCGATTTATGGCCTGATGGAAGATGCGGCGACGGCTGAAATTTCCCGTAC                  <  SRR4302543.1372810/94‑1 (MQ=60)
                                                                               GTGCAGTACATCGAGGCGTGGATCTCTGGCAACGGCTGTGTGCCGATTTATGGCCTGATGGAAGATGGGGCGACGGCTGAAATTTCCCGTACCT                >  SRR4302543.6202115/1‑94 (MQ=60)
                                                                               GTGCAGTACATCGAGGCGTGGATCTCTGGCAACGGCTGTGTGCCGATTTATGGCCTGATGGAAGATGCGGCGACGGCTGAAATTTCCCGTACCT                <  SRR4302543.5003830/94‑1 (MQ=60)
                                                                               GTGCAGTACATCGAGGCGTGGATCTCTGGCAACGGCTGTGTGCCGATTTATGGCCTGATGGAAGATGCGGCGACGGCTGAAATTTCCCGTACCT                >  SRR4302543.5104139/1‑94 (MQ=60)
                                                                                 GCAGTACATCGAGGCGTGGATCTCTGGCAACGGCTGTGTGCCGATTTATGGCCTGATGGAAGATGCGGCGACGGCTGAAATTTCCCGTACCTCG              <  SRR4302543.2092259/94‑1 (MQ=60)
                                                                                 GCAGTACATCGAGGCGTGGATCTCTGGCAACGGCTGTGTGCCGATTTATGGCCTGATGGAAGATGCGGCGACGGCTGAAATTTCCCGTACCTCG              <  SRR4302543.3459006/94‑1 (MQ=60)
                                                                                 GCAGTACATCGAGGCGTCGATCTCTGGCAACGGCTGTGTGCCGATTTATGGCCTGATGGAAGATGCGGCGACGGCTGAAATTTCCCGTACCTCG              <  SRR4302543.6263201/94‑1 (MQ=60)
                                                                                  CAGTACATCGAGGCGTGGATCTCTGGCAACGGCTGTGTGCCGATTTATGGCCTGATGGAAGATGCGGCGACGGCTGAAATTTCCCGTACCTCGA             >  SRR4302543.88229/1‑94 (MQ=60)
                                                                                   AGTACATCGAGGCGTGGATCTCTGGCAACGGCTGTGTGCCGATTTATGGCCTGATGGAAGATGCGGCGACGGCTGAAATTTCCCGTACCTCGAT            >  SRR4302543.1342437/1‑94 (MQ=60)
                                                                                   AGTACATCGAGGCGTGGATCTCTGGCAACGGCTGTGTGCCGATTTATGGCCTGATGGAAGATGCGGCGACGGCTGAAATTTCCCGTACCTCGAT            >  SRR4302543.5764558/1‑94 (MQ=60)
                                                                                    GTACATCGAGGCGTGGATCTCTGGCAACGGCTGTGTGCCGATTTATGGCCTGATGGAAGATGCGGCGACGGCTGAAATTTCCCGTACCTCGATC           <  SRR4302543.5542419/94‑1 (MQ=60)
                                                                                      ACATCGAGGCGTGGATCTCTGGCAACGGCTGTGTGCCGATTTATGGCCTGATGGAAGATGCGGCGACGGCTGAAATTTCCCGTACCTCGATCTG         <  SRR4302543.2978640/94‑1 (MQ=60)
                                                                                       CATCGAGGCGTGGATCTCTGGCAACGGCTGTGTGCCGATTTATGGCCTGATGGAAGATGCGGCGACGGCTGAAATTTCCCGTACCTCGATCTGG        <  SRR4302543.6073128/94‑1 (MQ=60)
                                                                                       CATCGAGGCGTGGATCTCTGGCAACGGCTGTGTGCCGATTTATGGCCTGATGGAAGATGCGGCGACGGCTGAAATTTCCCGTACCTCGATCTGG        >  SRR4302543.1444987/1‑94 (MQ=60)
                                                                                        ATCGAGGCGTGGATCTCTGGCAACGGCTGTGTGCCGATTTATGGCCTGATGGAAGATGCGGCGAGGGCTGAAATTTCCCGTACCTCGATGTGGC       >  SRR4302543.5415714/1‑94 (MQ=60)
                                                                                        ATCGAGGCGTGGATCTCTGGCAACGGCTGTGTGCCGATTTATGGCCTGATGGAAGATGCGGCGACGGCTGAAATTTCCCGTACCTCGATCTTGC       >  SRR4302543.1184397/1‑94 (MQ=60)
                                                                                        ATCGAGGCGTGGATCTCTGGCAACGGCTGTGTGCCGATTTATGGCCTGATGGAAGATGCGGCGACGGCTGAAATTTCCCGTACCTCGATCTGGC       >  SRR4302543.7850256/1‑94 (MQ=60)
                                                                                        ATCGAGGCGTGGATCTCTGGCAACGGCTGTGTGCCGATTTATGGCCTGATGGAAGATGCGGCGACGGCTGAAATTTCCCGTACCTCGATCTGGC       >  SRR4302543.7143086/1‑94 (MQ=60)
                                                                                         TCGAGGCGTGGATCTCTGGCAACGGCTGTGTGCCGATTTATGGCCTGATGGAAGATGCGGCGACGGCTGAAATTTCCCGTACCTCGATCTGGCA      <  SRR4302543.7446369/94‑1 (MQ=60)
                                                                                         TCGAGGCGTGGATCTCTGGCAACGGCTGTGTGCCGATTTATGGCCTGATGGAAGATGCGGCGACGGCTGAAATTTCCCGTACCTCGATCTGGCA      <  SRR4302543.4726911/94‑1 (MQ=60)
                                                                                          CGAGGCGTGGATCTCTGGCAACGGCTGTGTGCCGATTTATGGCCTGATGGAAGATGCGGCGACGGCTGAAATTTCCCGTACCTCGATCTGGCAG     <  SRR4302543.1885158/94‑1 (MQ=60)
                                                                                           GAGGCGTGGATCTCTGGCAACGGCTGTGTGCCGATTTATGGCCTGATGGAAGATGCGGCGACGGCTGAAATTTCCCGTACCTCGATCTGGCAGT    >  SRR4302543.151466/1‑94 (MQ=60)
                                                                                           GAGGCGTGGATCTCTGGCAACGGCTGTGTGCCGATTTATGGCCTGATGGAAGATGCGGCGACGGCTGAAATTTCCCGTACCTCGATCTGGCAGT    >  SRR4302543.1173561/1‑94 (MQ=60)
                                                                                            AGGCGTGGATCTCTGGCAACGGCTGTGTGCCGATTTATGGCCTGATGGAAGATGCGGCGACGGCTGAAATTTCCCGTACCTCGATCTGGCAGTG   >  SRR4302543.30572/1‑94 (MQ=60)
                                                                                            AGGCGTGGATCTCTGGCAACGGCTGTGTGCCGATTTATGGCCTGATGGAAGATGCGGCGACGGCTGAAATTTCCCGTACCTCGATCTGGCAGTG   <  SRR4302543.6732689/94‑1 (MQ=60)
                                                                                             GGCGTGGATCTCTGGCAACGGCTGTGTGCCGATTTATGGCCTGATGGAAGATGCGGCGACGGCTGAAATTTCCCGTACCTCGATCTGGCAGTGG  <  SRR4302543.1021525/94‑1 (MQ=60)
                                                                                             GGCGTGGATCTCTGGCAACGGCTGTGTGCCGATTTATGGCCTGATGGAAGATGCGGCGACGGCTGAAATTTCCCGTACCTCGATCTGGCAGTGG  <  SRR4302543.6257146/94‑1 (MQ=60)
                                                                                             |                                                                                             
GATTACTGCCGATCAGCTGCTGGCACCTTGTGATGGTGAACGCACCGAAGAAGGTATGCGCGCCAACATTCGCGTGGCTGTGCAGTACATCGAAGCGTGGATCTCTGGCAACGGCTGTGTGCCGATTTATGGCCTGATGGAAGATGCGGCGACGGCTGAAATTTCCCGTACCTCGATCTGGCAGTGG  >  NC_000913/4214253‑4214439

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 10 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑