Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 1873 183.1 9376148 98.2% 9207377 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,282,491 A→G V101A (GTA→GCA)  yjcH ← hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,282,4910AG99.6% 839.0 / ‑5.5 263V101A (GTA→GCA) yjcHhypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  major base G (124/138);  minor base C (0/1);  total (124/139)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01

Only 100 of 269 total aligned reads displayed.

CTCTACGGCCCCGCTAATAGCATCCGCGGCGTTAGCTGCGAAAGGGAGTGTGGCGGCAAGCGCCGTCAGAACTCTTTTCATGATGCTTGTACCTCATGCAGGACTTCATTATTAAGACGGTCGAATTCGCCGTTCGCCCGCCAGATGTAGATACCGGTGAGAACAAAGGAGATCACAATC  >  NC_000913/4282401‑4282580
                                                                                          |                                                                                         
ctctACGGCCCCGCTAATAGCATCCGCGGCGTTAGCTGCGAAAGGGAGTGTGGCGGCAAGCGCCGTCAGAACTCTTTTCATGATGCTTGTGcc                                                                                         >  2:1587675/1‑93 (MQ=255)
ctctACGGCCCCGCTAATAGCATCCGCGGCGTTAGCTGCGAAAGGGAGTGTGGCGGCAAGCGCCGTCAGAACTCTTTTCATGATGCTTGTGcc                                                                                         >  1:255932/1‑93 (MQ=255)
   tACGGCCCCGCTAATAGCATCCGCGGCGTTAGCTGCGAAAGGGAGTGTGGCGGCAAGCGCCGTCAGAACTCTTTTCATGATGCTTGTGCCTCa                                                                                      >  1:1514529/1‑93 (MQ=255)
    aCGGCCCCGCTAATAGCATCCGCGGCGTTAGCTGCGAAAGGGAGTGTGGCGGCAAGCGCCGTCAGAACTCTTTTCATGATGCTTGTGCCTCAt                                                                                     <  1:540852/93‑1 (MQ=255)
      ggCCCCGCTAATAGCATCCGCGGCGTTAGCTGCGAAAGGGAGTGTGGCGGCAAGCGCCGTCAGAACTCTTTTCATGATGCTTGTGCCTCATGc                                                                                   <  1:2459082/93‑1 (MQ=255)
          ccGCTAATAGCATCCGCGGCGTTAGCTGCGAAAGGGAGTGTGGCGGCAAGCGCCGTCAGAACTCTTTTCATGATGCTTGTGCCTCATGCAGGa                                                                               <  1:3721160/93‑1 (MQ=255)
           cGCTAATAGCATCCGCGGCGTTAGCTGCGAAAGGGAGTGTGGCGGCAAGCGCCGTCAGAACTCTTTTCATGATGCTTGTGCCTCATGCAGGAc                                                                              >  1:3065477/1‑93 (MQ=255)
           cGCTAATAGCATCCGCGGCGTTAGCTGCGAAAGGGAGTGTGGCGGCAAGCGCCGTCAGAACTCTTTTCATGATGCTTGTGCCTCATGCAGGAc                                                                              >  1:3671605/1‑93 (MQ=255)
           cGCTAATAGCATCCGCGGCGTTAGCTGCGAAAGGGAGTGTGGCGGCAAGCGCCGTCAGAACTCTTTTCATGATGCTTGTGCCTCATGCAGGAc                                                                              <  2:2242163/93‑1 (MQ=255)
           cGCTAATAGCATCCGCGGCGTTAGCTGCGAAAGGGAGTGTGGCGGCAAGCGCCGTCAGAACTCTTTTCATGATGCTTGTGCCTCATGCAGGAc                                                                              >  1:3898495/1‑93 (MQ=255)
            gCTAATAGCATCCGCGGCGTTAGCTGCGAAAGGGAGTGTGGCGGCAAGCGCCGTCAGAACTCTTTTCATGATGCTTGTGCCTCATGCAGGACt                                                                             >  2:2865558/1‑93 (MQ=255)
              tAATAGCATCCGCGGCGTTAGCTGCGAAAGGGAGTGTGGCGGCAAGCGCCGTCAGAACTCTTTTCATGATGCTTGTGCCTCATGCAGGACTTc                                                                           >  2:2546464/1‑93 (MQ=255)
              tAATAGCATCCGCGGCGTTAGCTGCGAAAGGGAGTGTGGCGGCAAGCGCCGTCAGAACTCTTTTCATGATGCTTGTGCCTCATGCAGGACTTc                                                                           >  1:2871019/1‑93 (MQ=255)
              tAATAGCATCCGCGGCGTTAGCTGCGAAAGGGAGTGTGGCGGCAAGCGCCGTCAGAACTCTTTTCATGATGCTTGTGCCTCATGCAGGACTTc                                                                           >  2:332555/1‑93 (MQ=255)
              tAATAGCATCCGCGGCGTTAGCTGCGAAAGGGAGTGTGGCGGCAAGCGCCGTCAGAACTCTTTTCATGATGCTTGTGCCTCATGCAGGACTTc                                                                           <  1:4202144/93‑1 (MQ=255)
              tAATAGCATCCGCGGCGTTAGCTGCGAAAGGGAGTGTGGCGGCAAGCGCCGTCAGAACTCTTTTCATGATGCTTGTGCCTCATGCAGGACTTc                                                                           <  2:2342177/93‑1 (MQ=255)
              tAATAGCATCCGCGGCGTTAGCTGCGAAAGGGAGTGTGGCGGCAAGCGCCGTCAGAACTCTTTTCATGATGCTTGTGCCTCATGCAGGACTTc                                                                           >  1:1315930/1‑93 (MQ=255)
                aTAGCATCCGCGGCGTTAGCTGCGAAAGGGAGTGTGGCGGCAAGCGCCGTCAGAACTCTTTTCATGATGCTTGTGCCTCATGCAGGACTTCat                                                                         >  1:1261932/1‑93 (MQ=255)
                aTAGCATCCGCGGCGTTAGCTGCGAAAGGGAGTGTGGCGGCAAGCGCCGTCAGAACTCTTTTCATGATGCTTGTGCCTCATGCAGGACTTCat                                                                         <  1:1080207/93‑1 (MQ=255)
                aTAGCATCCGCGGCGTTAGCTGCGAAAGGGAGTGTGGCGGCAAGCGCCGTCAGAACTCTTTTCATGATGCTTGTGCCTCATGCAGGACTTCat                                                                         >  1:1033061/1‑93 (MQ=255)
                 tAGCATCCGCGGCGTTAGCTGCGAAAGGGAGTGTGGCGGCAAGCGCCGTCAGAACTCTTTTCATGATGCTTGTCCCTCATGCAGGACTTCatt                                                                        <  2:4524772/93‑1 (MQ=255)
                  aGCATCCGCGGCGTTAGCTGCGAAAGGGAGTGTGGCGGCAAGCGCCGTCAGAACTCTTTTCATGATGCTTGTGCCTCATGCAGGACTTCatta                                                                       <  1:1731782/93‑1 (MQ=255)
                  aGCATCCGCGGCGTTAGCTGCGAAAGGGAGTGTGGCGGCAAGCGCCGTCAGAACTCTTTTCATGATGCTTGTGCCTCATGCAGGACTTCatta                                                                       <  2:1005146/93‑1 (MQ=255)
                     aTCCGCGGCGTTAGCTGCGAAAGGGAGTGTGGCGGCAAGCGCCGTCAGAACTCTTTTCATGATGCTTGTGCCTCATGCAGGACTTCAttatta                                                                    <  1:3473277/93‑1 (MQ=255)
                      tCCGCGGCGTTAGCTGCGAAAGGGAGTGTGGCGGCAAGCGCCGTCAGAACTCTTTTCATGATGCTTGTGCCTCATGCAGGACTTCATTATTaa                                                                   <  2:2724391/93‑1 (MQ=255)
                      tCCGCGGCGTTAGCTGCGAAAGGGAGTGTGGCGGCAAGCGCCGTCAGAACTCTTTTCATGATGCTTGTGCCTCATGCAGGACTTCATTATTaa                                                                   <  2:3553812/93‑1 (MQ=255)
                      tCCGCGGCGTTAGCTGCGAAAGGGAGTGTGGCGGCAAGCGCCGTCAGAACTCTTTTCATGATGCTTGTGCCTCATGCAGGACTTCATTATTaa                                                                   <  2:2136808/93‑1 (MQ=255)
                        cgcgGCGTTAGCTGCGAAAGGGAGTGTGGCGGCAAGCGCCGTCAGAACTCTTTTCATGATGCTTGTGCCTCATGCAGGACTTCATTATTAAGa                                                                 <  1:1862394/93‑1 (MQ=255)
                         gcggcgTTAGCTGCGAAAGGGAGTGTGGCGGCAAGCGCCGTCAGAACTCTTTTCATGATGCTTGTGCCTCATGCAGGACTTCATTATTAAGAc                                                                <  1:72521/93‑1 (MQ=255)
                          cggcgTTAGCTGCGAAAGGGAGTGTGGCGGCAAGCGCCGTCAGAACTCTTTTCATGATGCTTGTGCCTCATGCAGGACTTCATTATTAAGACg                                                               <  1:2259663/93‑1 (MQ=255)
                           ggcgTTAGCTGCGAAAGGGAGTGTGGCGGCAAGCGCCGTCAGAACTCTTTTCATGATGCTTGTGCCTCATGCAGGACTTCATTATTAAGACgg                                                              <  1:1117449/93‑1 (MQ=255)
                           ggcgTTAGCTGCGAAAGGGAGTGTGGCGGCAAGCGCCGTCAGAACTCTTTTCATGATGCTTGTGCCTCATGCAGGACTTCATTATTAAGACgg                                                              >  1:3800703/1‑93 (MQ=255)
                           ggcgTTAGCTGCGAAAGGGAGTGTGGCGGCAAGCGCCGTCAGAACTCTTTTCATGATGCTTGTGCCTCATGCAGGACTTCATTATTAAGACgg                                                              >  1:220042/1‑93 (MQ=255)
                           ggcgTTAGCTGCGAAAGGGAGTGTGGCGGCAAGCGCCGTCAGAACTCTTTTCATGATGCTTGTGCCTCATGCAGGACTTCATTATTAAGACgg                                                              <  2:2089351/93‑1 (MQ=255)
                            gcgTTAGCTGCGAAAGGGAGTGTGGCGGCAAGCGCCGTCAGAACTCTTTTCATGATGCTTGTGCCTCATGCAGGACTTCATTATTAAGACGGt                                                             <  1:1655135/93‑1 (MQ=255)
                            gcgTTAGCTGCGAAAGGGAGTGTGGCGGCAAGCGCCGTCAGAACTCTTTTCATGATGCTTGTGCCTCATGCAGGACTTCATTATTAAGACGGt                                                             <  2:1280866/93‑1 (MQ=255)
                            gcgTTAGCTGCGAAAGGGAGTGTGGCGGCAAGCGCCGTCAGAACTCTTTTCATGATGCTTGTGCCTCATGCAGGACTTCATTATTAAGACGGt                                                             >  2:3797878/1‑93 (MQ=255)
                            gcgTTAGCTGCGAAAGGGAGTGTGGCGGCAAGCGCCGTCAGAACTCTTTTCATGATGCTTGTGCCTCATGCAGGACTTCATTATTAAGACGGt                                                             <  2:2963793/93‑1 (MQ=255)
                              gTTAGCTGCGAAAGGGAGTGTGGCGGCAAGCGCCGTCAGAACTCTTTTCATGATGCTTGTGCCTCATGCAGGACTTCATTATTAAGACGGTCg                                                           >  1:910862/1‑93 (MQ=255)
                               ttAGCTGCGAAAGGGAGTGTGGCGGCAAGCGCCGTCAGAACTCTTTTCATGATGCTTGTGCCTCATGCAGGACTTCATTATTAAGACGGTCGa                                                          >  1:1000947/1‑93 (MQ=255)
                               ttAGCTGCGAAAGGGAGTGTGGCGGCAAGCGCCGTCAGAACTCTTTTCATGATGCTTGTGCCTCATGCAGGACTTCATTATTAAGACGGTCGa                                                          >  1:297219/1‑93 (MQ=255)
                               ttAGCTGCGAAAGGGAGTGTGGCGGCAAGCGCCGTCAGAACTCTTTTCATGATGCTTGTGCCTCATGCAGGACTTCATTATTAAGACGGTCGa                                                          <  1:3683023/93‑1 (MQ=255)
                                tAGCTGCGAAAGGGAGTGTGGCGGCAAGCGCCGTCAGAACTCTTTTCATGATGCTTGTGCCTCATGCAGGACTTCATTATTAAGACGGTCGaa                                                         <  1:2872537/93‑1 (MQ=255)
                                tAGCTGCGAAAGGGAGTGTGGCGGCAAGCGCCGTCAGAACTCTTTTCATGATGCTTGTGCCTCATGCAGGACTTCATTATTAAGACGGTCGaa                                                         <  2:725885/93‑1 (MQ=255)
                                 aGCTGCGAAAGGGAGTGTGGCGGCAAGCGCCGTCAGAACTCTTTTCATGATGCTTGTGCCTCATGCAGGACTTCATTATTAAGACGGTCGAAt                                                        <  2:3173155/93‑1 (MQ=255)
                                  gCTGCGAAAGGGAGTGTGGCGGCAAGCGCCGTCAGAACTCTTTTCATGATGCTTGTGCCTCATGCAGGACTTCATTATTAAGACGGTCGAAtt                                                       <  2:2054495/93‑1 (MQ=255)
                                  gCTGCGAAAGGGAGTGTGGCGGCAAGCGCCGTCAGAACTCTTTTCATGATGCTTGTGCCTCATGCAGGACTTCATTATTAAGACGGTCGAAtt                                                       >  1:3256328/1‑93 (MQ=255)
                                   cTGCGAAAGGGAGTGTGGCGGCAAGCGCCGTCAGAACTCTTTTCATGATGCTTGTGCCTCATGCAGGACTTCATTATTAAGACGGTCGAATTc                                                      <  1:1578722/93‑1 (MQ=255)
                                    tGCGAAAGGGAGTGTGGCGGCAAGCGCCGTCAGAACTCTTTTCATGATGCTTGTGCCTCATGCAGGACTTCATTATTAAGACGGTCGAATTCg                                                     <  2:1329173/93‑1 (MQ=255)
                                     gCGAAAGGGAGTGTGGCGGCAAGCGCCGTCAGAACTCTTTTCATGATGCTTGTGCCTCATGCAGGACTTCATTATTAAGACGGTCGAATTCGc                                                    >  1:1631914/1‑93 (MQ=255)
                                     gCGAAAGGGAGTGTGGCGGCAAGCGCCGTCAGAACTCTTTTCATGATGCTTGTGCCTCATGCAGGACTTCATTATTAAGACGGTCGAATTCGc                                                    >  2:1643821/1‑93 (MQ=255)
                                      cGAAAGGGAGTGTGGCGGCAAGCGCCGTCAGAACTCTTTTCATGATGCTTGTGCCTCATGCAGGACTTCATTATTAAGACGGTCGAATTCGcc                                                   <  1:2039592/93‑1 (MQ=255)
                                        aaaGGGAGTGTGGCGGCAAGCGCCGTCAGAACTCTTTTCATGATGCTTGTGCCTCATGCAGGACTTCATTATTAAGACGGTCGAATTCGCCGt                                                 <  1:3583694/93‑1 (MQ=255)
                                        aaaGGGAGTGTGGCGGCAAGCGCCGTCAGAACTCTTTTCATGATGCTTGTGCCTCATGCAGGACTTCATTATTAAGACGGTCGAATTCGCCGt                                                 >  1:2713869/1‑93 (MQ=255)
                                          aGGGAGTGTGGCGGCAAGCGCCGTCAGAACTCTTTTCATGATGCTTGTGCCTCATGCAGGACTTCATTATTAAGACGGTCGAATTCGCCGTTc                                               >  2:403104/1‑93 (MQ=255)
                                          aGGGAGTGTGGCGGCAAGCGCCGTCAGAACTCTTTTCATGATGCTTGTGCCTCATGCAGGACTTCATTATTAAGACGGTCGAATTCGCCGTTc                                               >  2:29981/1‑93 (MQ=255)
                                           gggAGTGTGGCGGCAAGCGCCGTCAGAACTCTTTTCATGATGCTTGTGCCTCATGCAGGACTTCATTATTAAGACGGTCGAATTCGCCGTTcg                                              >  2:3747984/1‑93 (MQ=255)
                                                    gcggcAAGCGCCGTCAGAACTCTTTTCATGATGCTTGTGCCTCATGCAGGACTTCATTATTAAGACGGTCGAATTCGCCGTTCGCCCGCCAGa                                     <  2:4639276/93‑1 (MQ=255)
                                                    gcggcAAGCGCCGACAGAACTCTTTTCATGATGCTTGTGCCTCATGCAGGACTTCATTATTAAGACGGTCGAATTCGCCGTTCGCCCGCCAGa                                     <  2:614070/93‑1 (MQ=255)
                                                       gcAAGCGCCGTCAGAACTCTTTTCATGATGCTTGTGCCTCATGCAGGACTTCATTATTAAGACGGTCGAATTCGCCGTTCGCCCGCCAGATGt                                  <  1:633786/93‑1 (MQ=255)
                                                         aaGCGCCGTCAGAACTCTTTTCATGATGCTTGTGCCTCATGCAGGACTTCATTATTAAGACGGTCGAATTCGCCGTTCGCCCGCCAGATGTAg                                >  2:3889853/1‑93 (MQ=255)
                                                         aaGCGCCGTCAGAACTCTTTTCATGATGCTTGTGCCTCATGCAGGACTTCATTATTAAGACGGTCGAATTCGCCGTTCGCCCGCCAGATGTAg                                >  1:4504971/1‑93 (MQ=255)
                                                          aGCGCCGTCAGAACTCTTTTCATGATGCTTGTGCCTCATGCAGGACTTCATTATTAAGACGGTCGAATTCGCCGTTCGCCCGCCAGATGTAGa                               <  1:2753204/93‑1 (MQ=255)
                                                           gcgcCGTCAGAACTCTTTTCATGATGCTTGTGCCTCATGCAGGACTTCATTATTAAGACGGTCGAATTCGCCGTTCGCCCGCCAGATGTAGAt                              <  2:502790/93‑1 (MQ=255)
                                                           gcgcCGTCAGAACTCTTTTCATGATGCTTGTGCCTCATGCAGGACTTCATTATTAAGACGGTCGAATTCGCCGTTCGCCCGCCAGATGTAGAt                              >  2:474455/1‑93 (MQ=255)
                                                           gcgcCGTCAGAACTCTTTTCATGATGCTTGTGCCTCATGCAGGACTTCATTATTAAGACGGTCGAATTCGCCGTTCGCCCGCCAGATGTAGAt                              <  1:329533/93‑1 (MQ=255)
                                                            cgcCGTCAGAACTCTTTTCATGATGCTTGTGCCTCATGCAGGACTTCATTATTAAGACGGTCGAATTCGCCGTTCGCCCGCCAGATGTAGATa                             <  1:4434809/93‑1 (MQ=255)
                                                               cGTCAGAACTCTTTTCATGATGCTTGTGCCTCATGCAGGACTTCATTATTAAGACGGTCGAATTCGCCGTTCGCCCGCCAGATGTAGATACCg                          >  1:3277092/1‑93 (MQ=255)
                                                                   aGAACTTTTTTCATGATGCTTGTGCCTCATGCAGGACTTCATTATTAAGACGGTCGAATTCGCCGTTCGCCCGCCAGATGTAGATACCGGTga                      >  1:4082193/1‑93 (MQ=255)
                                                                   aGAACTCTTTTCATGATGCTTGTGCGTCATGCAGTACTTCATTATTAAGACGGTCGAATTCGCCGTTCGCCCGCCAGATGTAGATACCGGTga                      <  2:2621320/93‑1 (MQ=255)
                                                                   aGAACTCTTTTCATGATGCTTGTGCCTCATGCAGGACTTCATTATTAAGACGGTCGAATTCGCCGTTCGCCCGCCAGATGTAGATACCGGTga                      <  2:1146790/93‑1 (MQ=255)
                                                                   aGAACTCTTTTCATGATGCTTGTGCCTCATGCAGGACTTCATTATTAAGACGGTCGAATTCGCCGTTCGCCCGCCAGATGTAGATACCGGTga                      >  2:1025472/1‑93 (MQ=255)
                                                                   aGAACTCTTTTCATGATGCTTGTGCCTCATGCAGGACTTCATTATTAAGACGGTCGAATTCGCAGTTCGCCCGCCAGATGTAGATACCGGTga                      >  2:3202759/1‑93 (MQ=255)
                                                                    gAACTCTTTTCATGATGCTTGTGCCTCATGCAGGACTTCATTATTAAGACGGTCGAATTCGCCGTTCGCCCGCCAGATGTAGATACCGGTgag                     >  2:4180777/1‑93 (MQ=255)
                                                                      aCTCTTTTCATGATGCTTGTGCCTCATGCAGGACTTCATTATTAAGACGGTCGAATTCGCCGTTCGCCCGCCAGATGTAGATACCGGTGAGaa                   >  1:4669281/1‑93 (MQ=255)
                                                                       ctctTTTCATGATGCTTGTGCCTCATGCAGGACTTCATTATTAAGACGGTCGAATTCGCCGTTCGCCCGCCAGATGTAGATACCGGTGAGAAc                  <  2:794338/93‑1 (MQ=255)
                                                                        tctTTTCATGATGCTTGTGCCTCATGCAGGACTTCATTATTAAGACGGTCGAATTCGCCGTTCGCCCGCCAGATGTAGATACCGGTGAGAACa                 >  1:3412399/1‑93 (MQ=255)
                                                                         ctTTGCATAATGCTTGTGCCTCATGCAGGACTTCATTATTAATACGGTCGAATTCGCCGTTCGCCCGCCAGATGTAGATACCGGTGAGAACaa                <  2:4324764/93‑1 (MQ=255)
                                                                          ttttCATGATGCTTGTGCCTCATGCAGGACTTCATTATTAAGACGGTCGAATTCGCCGTTCGCCCGCCAGATGTAGATACCGGTGAGAACaaa               <  2:3216706/93‑1 (MQ=255)
                                                                          ttttCATGATGCTTGTGCCTCATGCAGGACTTCATTATTAAGACGGTCGAATTCGCCGTTCGCCCGCCAGATGTAGATACCGGTGAGAACaaa               >  1:610862/1‑93 (MQ=255)
                                                                          ttttCATGATGCTTGTGCCTCATGCAGGACTTCATTATTAAGACGGTCGAATTCGCCGTTCGCCCGCCAGATGTAGATACCGGTGAGAACaaa               <  1:1224923/93‑1 (MQ=255)
                                                                          ttttCATGATGCTTGTGCCTCATGCAGGACTTCATTATTAAGACGGTCGAATTCGCCGTTCGCCCGCCAGATGTAGATACCGGTGAGAACaaa               >  2:3697997/1‑93 (MQ=255)
                                                                           tttCATGATGCTTGTGCCTCATGCAGGACTTCATTATTAAGACGGTCGAATTCGCCGTTCGCCCGCCAGATGTAGATACCGGTGAGAACAAAg              <  1:4139014/93‑1 (MQ=255)
                                                                             tCATGATGCTTGTGCCTCATGCAGGACTTCATTATTAAGACGGTCGAATTCGCCGTTCGCCCGCCAGATGTAGATACCGGTGAGAACAAAGga            <  1:4299890/93‑1 (MQ=255)
                                                                               atgatgCTTGTGCCTCATGCAGGACTTCATTATTAAGACGGTCGAATTTGCCGTTCGCCCGCCAGATGTAGATACCGGTGAGAACAAAGgaga          >  2:1173035/1‑93 (MQ=255)
                                                                               atgatgCTTGTGCCTCATGCAGGACTTCATTATTAAGACGGTCGAATTCGCCGTTCGCCCGCCAGATGTAGATACCGGTGAGAACAAAGgaga          >  2:3514311/1‑93 (MQ=255)
                                                                               atgatgCTTGTGCCTCATGCAGGACTTCATTATTAAGACGGTCGAATTCGCCGTTCGCCCGCCAGATGTAGATACCGGTGAGAACAAAGgaga          >  1:1976054/1‑93 (MQ=255)
                                                                                tgatgCTTGTGCCTCATGCAGGACTTCATTATTAAGACGGTCGAATTCGCCGTTCGCCCGCCAGATGTAGATACCGGTGAGAACAAAGGAGAt         <  1:735451/93‑1 (MQ=255)
                                                                                tgatgCTTGTGCCTCATGCAGGACTTCATTATTAAGACGGTCGAATTCGCCGTTCGCCCGCCAGATGTAGATACCGGTGAGAACAAAGGAGAt         >  2:585693/1‑93 (MQ=255)
                                                                                tgatgCTTGTGCCTCATGCAGGACTTCATTATTAAGACGGTCGAATTCGCCGTTCGCCCGCCAGATGTAGATACCGGTGAGAACAAAGGAGAt         <  2:1504451/93‑1 (MQ=255)
                                                                                 gatgCTTGTGCCTCATGCAGGACTTCATTATTAAGACGGTCGAATTCGCCGTTCGCCCGCCAGATGTAGATACCGGTGAGAACAAAGGAGATc        <  1:1437553/93‑1 (MQ=255)
                                                                                   tgCTTGTGCCTCATGCAGGACTTCATTATTAAGACGGTCGAATTCGCCGTTCGCCCGCCAGATGTAGATACCGGTGAGAACAAAGGAGATcac      >  2:3976214/1‑93 (MQ=255)
                                                                                   tgCTTGTGCCTCATGCAGGACTTCATTATTAAGACGGTCGAATTCGCCGTTCGCCCGCCAGATGTAGATACCGGTGAGAACAAAGGAGATcac      >  1:195017/1‑93 (MQ=255)
                                                                                   tgCTTGTGCCTCATGCAGGACTTCATTATTAAGACGGTCGAATTCGCCGTTCGCCCGCCAGATGTAGATACCGGTGAGAACAAAGGAGATcac      >  2:929870/1‑93 (MQ=255)
                                                                                    gCTTGTGCCTCATGCAGGACTTCATTATTAAGACGGTCGAATTCGCCGTTCGCCCGCCAGATGTAGATACCGGTGAGAACAAAGGAGATcaca     <  1:1781261/93‑1 (MQ=255)
                                                                                     cTTGTGCCTCATGCAGGACTTCATTATTAAGACGGTCGAATTCGCCGTTCGCCCGCCAGATGTAGATACCGGTGAGAACAAAGGAGATCACaa    >  2:3272958/1‑93 (MQ=255)
                                                                                      ttGTGCCTCATGCAGGACTTCATTATTAAGACGGTCGAATTCGCCGTTCGCCCGCCAGATGTAGATACCGGTGAGAACAAAGGAGATCACAAt   <  1:2659269/93‑1 (MQ=255)
                                                                                      ttGTGCCTCATGCAGGACTTCATTATTAAGACGGTCGAATTCGCCGTTCGCCCGCCAGATGTAGATACCGGTGAGAACAAAGGAGATCACAAt   >  1:4395893/1‑93 (MQ=255)
                                                                                      ttGTGCCTCATGCAGGACTTCATTATTAAGACGGTCGAATTCGCCGTTCGCCCGCCAGATGTAGATACCGGTGAGAACAAAGGAGATCACAAt   >  2:2265692/1‑93 (MQ=255)
                                                                                      ttGTGCCTCATGCAGGACTTCATTATTAAGACGGTCGAATTCGCCGTTCGCCCGCCAGATGTAGATACCGGTGAGAACAAAGGAGATCACAAt   >  2:1979881/1‑93 (MQ=255)
                                                                                          |                                                                                         
CTCTACGGCCCCGCTAATAGCATCCGCGGCGTTAGCTGCGAAAGGGAGTGTGGCGGCAAGCGCCGTCAGAACTCTTTTCATGATGCTTGTACCTCATGCAGGACTTCATTATTAAGACGGTCGAATTCGCCGTTCGCCCGCCAGATGTAGATACCGGTGAGAACAAAGGAGATCACAATC  >  NC_000913/4282401‑4282580

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GCGCTCTACGGCCCCGCTAATAGCATCCGCGGCGTTAGCTGCGAAAGGGAGTGTGGCGGCAAGCGCCGTCAGAACTCTTTTCATGATGCTTGTACCTCATGCAGGACTTCATTATTAAGACGGTCGAATTCGCCGTTCGCCCGCCAGATGTAGATACCGGTGAGAACAAAGGAGATCACAATCACTC  >  NC_000913/4282398‑4282584
                                                                                             |                                                                                             
GCGCTCTACGGCCCCGCTAATAGCATCCGCGGCGTTAGCTGCGAAAGGGAGTGTGGCGGCAAGCGCCGTCAGAACTCTTTTCATGATGCTTGTG                                                                                               <  SRR4302535.3757385/94‑1 (MQ=60)
 CGCTCTACGGCCCCGCTAATAGCATCCGCGGCGTTAGCTGCGAAAGGGAGTGTGGCGGCAAGCGCCGTCAGAACTCTTTTCATGATGCTTGTGC                                                                                              >  SRR4302535.318365/1‑94 (MQ=60)
  GCTCTACGGCCCCGCTAATAGCATCCGCGGCGTTAGCTGCGAAAGGGAGTGTGGCGGCAAGCGCCGTCAGAACTCTTTTCATGATGCTTGTGCC                                                                                             >  SRR4302535.257695/1‑94 (MQ=60)
   CTCTACGGCCCCGCTAATAGCATCCGCGGCGTTAGCTGCGAAAGGGAGTGTGGCGGCAAGCGCCGTCAGAACTCTTTTCATGATGCTTGTGCCT                                                                                            <  SRR4302535.3432006/94‑1 (MQ=60)
     CTACGGCCCCGCTAATAGCATCCGCGGCGTTAGCTGCGAAAGGGAGTGTGGCGGCAAGCGCCGTCAGAACTCTTTTCATGATGCTTGTGCCTCA                                                                                          >  SRR4302535.1525437/1‑94 (MQ=60)
     CTACGGCCCCGCTAATAGCATCCGCGGCGTTAGCTGCGAAAGGGAGTGTGGCGGCAAGCGCCGTCAGAACTCTTTTCATGATGCTTGTGCCTCA                                                                                          <  SRR4302535.1653265/94‑1 (MQ=60)
       ACGGCCCCGCTAATAGCATCCGCGGCGTTAGCTGCGAAAGGGAGTGTGGCGGCAAGCGCCGTCAGAACTCTTTTCATGATGCTTGTGCCTCATG                                                                                        <  SRR4302535.4719572/94‑1 (MQ=60)
       ACGGCCCCGCTAATAGCATCCGCGGCGTTAGCTGCGAAAGGGAGTGTGGCGGCAAGCGCCGTCAGAACTCTTTTCATGATGCTTGTGCCTCATG                                                                                        <  SRR4302535.544629/94‑1 (MQ=60)
         GGCCCCGCTAATAGCATCCGCGGCGTTAGCTGCGAAAGGGAGTGTGGCGGCAAGCGCCGTCAGAACTCTTTTCATGATGCTTGTGCCTCATGCA                                                                                      <  SRR4302535.2476817/94‑1 (MQ=60)
         GGCCCCGCTAATAGCATCCGCGGCGTTAGCTGCGAAAGGGAGTGTGGCGGCAAGCGCCGTCAGAACTCTTTTCATGATGCTTGTGCCTCATGCA                                                                                      <  SRR4302535.86872/94‑1 (MQ=60)
            CCCGCTAATAGCATCCGCGGCGTTAGCTGCGAAAGGGAGTGTGGCGGCAAGCGCCGTCAGAACTCTTTTCATGATGCTTGTGCCTCATGCAGGA                                                                                   >  SRR4302535.1301045/1‑94 (MQ=60)
             CCGCTAATAGCATCCGCGGCGTTAGCTGCGAAAGGGAGTGTGGCGGCAAGCGCCGTCAGAACTCTTTTCATGATGCTTGTGCCTCATGCAGGAC                                                                                  >  SRR4302535.3697033/1‑94 (MQ=60)
             CCGCTAATAGCATCCGCGGCGTTAGCTGCGAAAGGGAGTGTGGCGGCAAGCGCCGTCAGAACTCTTTTCATGATGCTTGTGCCTCATGCAGGAC                                                                                  >  SRR4302535.3086904/1‑94 (MQ=60)
             CCGCTAATAGCATCCGCGGCGTTAGCTGCGAAAGGGAGTGTGGCGGCAAGCGCCGTCAGAACTCTTTTCATGATGCTTGTGCCTCATGCAGGAC                                                                                  <  SRR4302535.3746939/94‑1 (MQ=60)
             CCGCTAATAGCATCCGCGGCGTTAGCTGCGAAAGGGAGTGTGGCGGCAAGCGCCGTCAGAACTCTTTTCATGATGCTTGTGCCTCATGCAGGAC                                                                                  >  SRR4302535.3925202/1‑94 (MQ=60)
                CTAATAGCATCCGCGGCGTTAGCTGCGAAAGGGAGTGTGGCGGCAAGCGCCGTCAGAACTCTTTTCATGATGCTTGTGCCTCATGCAGGACTTC                                                                               >  SRR4302535.2891360/1‑94 (MQ=60)
                CTAATAGCATCCGCGGCGTTAGCTGCGAAAGGGAGTGTGGCGGCAAGCGCCGTCAGAACTCTTTTCATGATGCTTGTGCCTCATGCAGGACTTC                                                                               >  SRR4302535.1325328/1‑94 (MQ=60)
                CTAATAGCATCCGCGGCGTTAGCTGCGAAAGGGAGCGTGGCGGCAAGCGCCGTCAGAACTCTTTTCATGATGCTTGTGCCTCATGCAGGACTTC                                                                               >  SRR4302535.2891361/1‑94 (MQ=60)
                 TAATAGCATCCGCGGCGTTAGCTGCGAAAGGGAGTGTGGCGGCAAGCGCCGTCAGAACTCTTTTCATGATGCTTGTGCCTCATGCAGGACTTCA                                                                              <  SRR4302535.4230893/94‑1 (MQ=60)
                  AATAGCATCCGCGGCGTTAGCTGCGAAAGGGAGTGTGGCGGCAAGCGCCGTCAGAACTCTTTTCATGATGCTTGTGCCTCATGCAGGACTTCAT                                                                             <  SRR4302535.937829/94‑1 (MQ=60)
                  AATAGCATCCGCGGCGTTAGCTGCGAAAGGGAGTGTGGCGGCAAGCGCCGTCAGAACTCTTTTCATGATGCTTGTGCCTCATGCAGGACTTCAT                                                                             <  SRR4302535.1466029/94‑1 (MQ=60)
                  AATAGCATCCGCGGCGTTAGCTGCGAAAGGGAGTGTGGCGGCAAGCGCCGTCAGAACTCTTTTCATGATGCTTGTGCCTCATGCAGGACTTCAT                                                                             >  SRR4302535.1270961/1‑94 (MQ=60)
                  AATAGCATCCGCGGCGTTAGCTGCGAAAGGGAGTGTGGCGGCAAGCGCCGTCAGAACTCTTTTCATGATGCTTGTGCCTCATGCAGGACTTCAT                                                                             >  SRR4302535.1095029/1‑94 (MQ=60)
                  AATAGCATCCGCGGCGTTAGCTGCGAAAGGGAGTGTGGCGGCAAGCGCCGTCAGAACTCTTTTCATGATGCTTGTGCCTCATGCAGGACTTCAT                                                                             >  SRR4302535.1040402/1‑94 (MQ=60)
                   ATAGCATCCGCGGCGTTAGCTGCGAAAGGGAGTGTGGCGGCAAGCGCCGTCAGAACTCTTTTCATGATGCTTGTGCCTCATGCAGGACTTCATT                                                                            <  SRR4302535.1087892/94‑1 (MQ=60)
                   ATAGCATCCGCGGCGTTAGCTGCGAAAGGGAGTGTGGCGGCAAGCGCCGTCAGAACTCTTTTCATGATGCTTGTGCCTCATGCAGGACTTCATT                                                                            <  SRR4302535.4131670/94‑1 (MQ=60)
                   ATAGCATCCGCGGCGTTAGCTGCGAAAGGGAGTGTGGCGGCAAGCGCCGTCAGAACTCTTTTCATGATGCTTGTGCCTCATGCAGGACTTCATT                                                                            >  SRR4302535.1758125/1‑94 (MQ=60)
                    TAGCATCCGCGGCGTTAGCTGCGAAAGGGAGTGTGGCGGCAAGCGCCGTCAGAACTCTTTTCATGATGCTTGTGCCTCATGCAGGACTTCATTA                                                                           <  SRR4302535.4396659/94‑1 (MQ=60)
                    TAGCATCCGCGGCGTTAGCTGCGAAAGGGAGTGTGGCGGCAAGCGCCGTCAGAACTCTTTTCATGATGCTTGTGCCTCATGCAGGACTTCATTA                                                                           <  SRR4302535.183837/94‑1 (MQ=60)
                    TAGCATCCGCGGCGTTAGCTGCGAAAGGGAGTGTGGCGGCAAGCGCCGTCAGAACTCTTTTCATGATGCTTGTGCCTCATGCAGGACTTCATTA                                                                           >  SRR4302535.1241222/1‑94 (MQ=60)
                     AGCATCCGCGGCGTTAGCTGCGAAAGGGAGTGTGGCGGCAAGCGCCGTCAGAACTCTTTTCATGATGCTTGTGCCTCATGCAGGACTTCATTAT                                                                          >  SRR4302535.1568216/1‑94 (MQ=60)
                     AGCATCCGCGGCGTTAGCTGCGAAAGGGAGTGTGGCGGCAAGCGCCGTCAGAACTCTTTTCATGATGCTTGTGCCTCATGCAGGACTTCATTAT                                                                          >  SRR4302535.4465237/1‑94 (MQ=60)
                     AGCATCCGCGGCGTTAGCTGCGAAAGGGAGTGTGGCGGCAAGCGCCGTCAGAACTCTTTTCATGATGCTTGTGCCTCATGCAGGACTTCATTAT                                                                          <  SRR4302535.1744301/94‑1 (MQ=60)
                      GCATCCGCGGCGTTAGCTGCGAAAGGGAGTGTGGCGGCAAGCGCCGTCAGAACTCTTTTCATGATGCTTGTGCCTCATGCAGGACTTCATTATT                                                                         <  SRR4302535.3965057/94‑1 (MQ=60)
                      GCATCCGCGGCGTTAGCTGCGAAAGGGAGTGTGGCAGCAAGCGCCGTCAGAACTCTTTTCATGATGCTTGTGCCTCATGCAGGACTTCATTATT                                                                         >  SRR4302535.4143963/1‑94 (MQ=60)
                       CATCCGCGGCGTTAGCTGCGAAAGGGAGTGTGGCGGCAAGCGCCGTCAGAACTCTTTTAATGATGCTTGTGCCTCATGCAGGACTTCATTATTA                                                                        >  SRR4302535.3793528/1‑94 (MQ=60)
                        ATCCGCGGCGTTAGCTGCGAAAGGGAGTGTGGCGGCAAGCGCCGTCAGAACTCTTTTCATGATGCTTGTGCCTCATGCAGGACTTCATTATTAA                                                                       <  SRR4302535.3497411/94‑1 (MQ=60)
                        ATCCGCGGCGTTAGCTGCGAAAGGGAGTGTGGCGGCAAGCGCCGTCAGAACTCTTTTCATGATGCTTGTGCCTCATGCAGGACTTCATTATTAA                                                                       >  SRR4302535.1978994/1‑94 (MQ=60)
                        ATCCGCGGCGTTAGCTGCGAAAGGGAGTGTGGCGGCAAGCGCCGTCAGAACTCTTTTCATGATGCTTGTGCCTCATGCAGGACTTCATTATTAA                                                                       <  SRR4302535.2937110/94‑1 (MQ=60)
                         TCCGCGGCGTTAGCTGCGAAAGGGAGTGTGGCGGCAAGCGCCGTCAGAACTCTTTTCATGATGCTTGTGCCTCATGCAGGACTTCATTATTAAG                                                                      <  SRR4302535.1253768/94‑1 (MQ=60)
                           CGCGGCGTTAGCTGCGAAAGGGAGTGTGGCGGCAAGCGCCGTCAGAACTCTTTTCATGATGCTTGTGCCTCATGCAGGACTTCATTATTAAGAC                                                                    <  SRR4302535.1875826/94‑1 (MQ=60)
                           CGCGGCGTTAGCTGCGAAAGGGAGTGTGGCGGCAAGCGCCGTCAGAACTCTTTTCATGATGCTTGTGCCTCATGCAGGACTTCATTATTAAGAC                                                                    <  SRR4302535.2623416/94‑1 (MQ=60)
                            GCGGCGTTAGCTGCGAAAGGGAGTGTGGCGGCAAGCGCCGTCAGAACTCTTTTCATGATGCTTGTGCCTCATGCAGGACTTCATTATTAAGACG                                                                   <  SRR4302535.3714107/94‑1 (MQ=60)
                            GCGGCGTTAGCTGCGAAAGGGAGTGTGGCGGCAAGCGCCGTCAGAACTCTTTTCATGATGCTTGTGCCTCATGCAGGACTTCATTATTAAGACG                                                                   <  SRR4302535.73048/94‑1 (MQ=60)
                             CGGCGTTAGCTGCGAAAGGGAGTGTGGCGGCAAGCGCCGTCAGAACTCTTTTCATGATGCTTGTGCCTCATGCAGGACTTCATTATTAAGACGG                                                                  >  SRR4302535.221557/1‑94 (MQ=60)
                             CGGCGTTAGCTGCGAAAGGGAGTGTGGCGGCAAGCGCCGTCAGAACTCTTTTCATGATGCTTGTGCCTCATGCAGGACTTCATTATTAAGACGG                                                                  <  SRR4302535.2275753/94‑1 (MQ=60)
                             CGGCGTTAGCTGCGAAAGGGAGTGTGGCGGCAAGCGCCGTCAGAACTCTTTTCATGATGCTTGTGCCTCATGCAGGACTTCATTATTAAGACGG                                                                  <  SRR4302535.1092792/94‑1 (MQ=60)
                             CGGCGTTAGCTGCGAAAGGGAGTGTGGCGGCAAGCGCCGTCAGAACTCTTTTCATGATGCTTGTGCCTCATGCAGGACTTCATTATTAAGACGG                                                                  >  SRR4302535.3826902/1‑94 (MQ=60)
                              GGCGTTAGCTGCGAAAGGGAGTGTGGCGGCAAGCGCCGTCAGAACTCTTTTCATGATGCTTGTGCCTCATGCAGGACTTCATTATTAAGACGGT                                                                 <  SRR4302535.1125419/94‑1 (MQ=60)
                              GGCGTTAGCTGCGAAAGGGAGTGTGGCGGCAAGCGCCGTCAGAACTCTTTTCATGATGCTTGTGCCTCATGCAGGACTTCATTATTAAGACGGT                                                                 >  SRR4302535.2270876/1‑94 (MQ=60)
                               GCGTTAGCTGCGAAAGGGAGTGTGGCGGCAAGCGCCGTCAGAACTCTTTTCATGATGCTTGTGCCTCATGCAGGACTTCATTATTAAGACGGTC                                                                <  SRR4302535.1667107/94‑1 (MQ=60)
                                CGTTAGCTGCGAAAGGGAGTGTGGCGGCAAGCGCCGTCAGAACTCTTTTCATGATGCTTGTGCCTCATGCAGGACTTCATTATTAAGACGGTCG                                                               >  SRR4302535.917339/1‑94 (MQ=60)
                                CGTTAGCTGCGAAAGGGAGTGTGGCGGCAAGCGCCGTCAGAACTCTTTTCATGATGCTTGTGCCTCATGCAGGACTTCATTATTAAGACGGTCG                                                               <  SRR4302535.1017162/94‑1 (MQ=60)
                                CGTTAGCTGCGAAAGGGAGTGTGGCGGCAAGCGCCGTCAGAACTCTTTTCATGATGCTTGTGCCTCATGCAGGACTTCATTATTAAGACGGTCG                                                               >  SRR4302535.194142/1‑94 (MQ=60)
                                 GTTAGCTGCGAAAGGGAGTGTGGCGGCAAGCGCCGTCAGAACTCTTTTCATGATGCTTGTGCCTCATGCAGGACTTCATTATTAAGACGGTCGA                                                              >  SRR4302535.1008061/1‑94 (MQ=60)
                                 GTTAGCTGCGAAAGGGAGTGTGGCGGCAAGCGCCGTCAGAACTCTTTTCATGATGCTTGTGCCTCATGCAGGACTTCATTATTAAGACGGTCGA                                                              >  SRR4302535.299291/1‑94 (MQ=60)
                                  TTAGCTGCGAAAGGGAGTGTGGCGGCAAGCGCCGTCAGAACTCTTTTCATGATGCTTGTGCCTCATGCAGGACTTCATTATTAAGACGGTCGAA                                                             <  SRR4302535.3708509/94‑1 (MQ=60)
                                   TAGCTGCGAAAGGGAGTGTGGCGGCAAGCGCCGTCAGAACTCTTTTCATGATGCTTGTGCCTCATGCAGGACTTCATTATTAAGACGGTCGAAT                                                            <  SRR4302535.933091/94‑1 (MQ=60)
                                   TAGCTGCGAAAGGGAGTGTGGCGGCAAGCGCCGTCAGAACTCTTTTCATGATGCTTGTGCCTCATGCAGGACTTCATTATTAAGACGGTCGAAT                                                            <  SRR4302535.2892893/94‑1 (MQ=60)
                                    AGCTGCGAAAGGGAGTGTGGCGGCAAGCGCCGTCAGAACTCTTTTCATGATGCTTGTGCCTCATGCAGGACTTCATTATTAAGACGGTCGAATT                                                           >  SRR4302535.3278953/1‑94 (MQ=60)
                                    AGCTGCGAAAGGGAGTGTGGCGGCAAGCGCCGTCAGAACTCTTTTCATGATGCTTGTGCCTCATGCAGGACTTCATTATTAAGACGGTCGAATT                                                           <  SRR4302535.3132958/94‑1 (MQ=60)
                                     GCTGCGAAAGGGAGTGTGGCGGCAAGCGCCGTCAGAACTCTTTTCATGATGCTTGTGCCTCATGCAGGACTTCATTATTAAGACGGTTGAATTC                                                          <  SRR4302535.2787502/94‑1 (MQ=60)
                                      CTGCGAAAGGGAGTGTGGCGGCAAGCGCCGTCAGAACTCTTTTCATGATGCTTGTGCCTCATGCAGGACTTCATTATTAAGACGGTCGAATTCG                                                         <  SRR4302535.1590120/94‑1 (MQ=60)
                                       TGCGAAAGGGAGTGTGGCGGCAAGCGCCGTCAGAACTCTTTTCATGATGCTTGTGCCTCATGCAGGACTTCATTATTAAGACGGTCGAATTCGC                                                        >  SRR4302535.1643705/1‑94 (MQ=60)
                                         CGAAAGGGAGTGTGGCGGCAAGCGCCGTCAGAACTCTTTTCATGATGCTTGTGCCTCATGCAGGACTTCATTATTAAGACGGTCGAATTCGCCG                                                      >  SRR4302535.4314256/1‑94 (MQ=60)
                                         CGAAAGGGAGTGTGGCGGCAAGCGCCGTCAGAACTCTTTTCATGATGCTTGTGCCTCATGCAGGACTTCATTATTAAGACGGTCGAATTCGCCG                                                      <  SRR4302535.2054282/94‑1 (MQ=60)
                                         CGAAAGGGAGTGTGGCGGCAAGCGCCGTCAGAACTCTTTTCATGATGCTTATGCCTCATGCAGGACTTCATTATTAAGACGGTCGAATTCGCCG                                                      <  SRR4302535.3324682/94‑1 (MQ=60)
                                          GAAAGGGAGTGTGGCGGCAAGCGCCGTCAGAACTCTTTTCATGATGCTTGTGCCTCATGCAGGACTTCATTATTAAGACGGTCGAATTCGCCGT                                                     >  SRR4302535.2733257/1‑94 (MQ=60)
                                           AAAGGGAGTGTGGCGGCAAGCGCCGTCAGAACTCTTTTCATGATGCTTGTGCCTCATGCAGGACTTCATTATTAAGACGGTCGAATTCGCCGTT                                                    <  SRR4302535.3608548/94‑1 (MQ=60)
                                            AAGGGAGTGTGGCGGCAAGCGCCGTCAGAACTCTTTTCATGATGCTTGTGCCTCATGCAGGACTTCATTACTAAGACGGTCGAATTCGCCGTTC                                                   <  SRR4302535.2150182/94‑1 (MQ=60)
                                             AGGGAGTGTGGCGGCAAGCGCCGTCAGAACTCTTTTCATGATGCTTGTGCCTCATGCAGGACTTCATTATTAAGACGGTCGAATTCGCCGTTCG                                                  >  SRR4302535.3651747/1‑94 (MQ=60)
                                              GGGAGTGTGGCGGCAAGCGCCGTCAGAACTCTTTTCATGATGCTTGAGCCTCATGCAGGACTTCATTATTAAGACGGTCGAATTCGCCGTTCGC                                                 >  SRR4302535.2267903/1‑94 (MQ=60)
                                                    GTGGCGGCAAGCGCCGTCAGACCTCTTTTCATGATGCTTGTGCCTCATGCAGGACTTCATTATTAAGACGGTCGAATTCGCCGTTCGCCCGCCA                                           <  SRR4302535.4381437/94‑1 (MQ=60)
                                                    GTGGCGGCAAGCGCCGTCAGAACTCTTTTCATGATGCTTGTGCCTCATGCAGGACTTCATTATTAAGACGGTCGAATTCGCCGTTCGCCCGCCA                                           <  SRR4302535.2907522/94‑1 (MQ=60)
                                                    GTGGCGGCAAGCGCCGTCAGAACTCTTTTCATGATGCTTGTGCCTCATGCAGGACTTCATTATTAAGACGGTCGAATTCGCCGTTCGCCCGCCA                                           <  SRR4302535.3024201/94‑1 (MQ=60)
                                                       GCGGCAAGCGCCGTCAGAACTCTTTTCATGATGCTTGTGCCTCATGCAGGACTTCATTATTAAGACGGTCGAATTCGCCGTTCGCCCGCCAGAT                                        >  SRR4302535.729285/1‑94 (MQ=60)
                                                       GCGGCAAGCGCCGTCAGAACTCTTTTCATGATGCTTGTGCCTCATGCAGGACTTCATTATTAAGACGGTCGAATTCGCCGTTCGCCCGCCAGAT                                        <  SRR4302535.1738226/94‑1 (MQ=60)
                                                       GCGGCAAGCGCCGTCAGAACTCTTTTCATGATGCTTGTGCCTCATGCAGGACTTCATTATTAAGACGGTCGAATTCGCCGTTCGCCCGCCAGAT                                        <  SRR4302535.2747746/94‑1 (MQ=60)
                                                        CGGCAAGCGCCGTCAGAACTCTTTTCATGATGCTTGTGCCTCATGCAGGACTTCATTATTAAGACGGTCGAATTCGCCGTTCGCCCGCCAGATG                                       <  SRR4302535.3718857/94‑1 (MQ=60)
                                                        CGGCAAGCGCCGTCAGAACTCTTTTCATGATGCTTGTGCCTCATGCAGGACTTCATTATTAAGACGGTCGAATTCGCCGTTCGCCCGCCAGATG                                       <  SRR4302535.466446/94‑1 (MQ=60)
                                                         GGCAAGCGCCGTCAGAACTCTTTTCATGATGCTTGTGCCTCATGCAGGACTTCATTATTAAGACGGTCGAATTCGCCGTTCGCCCGCCAGATGT                                      <  SRR4302535.3593065/94‑1 (MQ=60)
                                                          GCAAGCGCCGTCAGAACTCTTTTCATGATGCTTGTGCCTCATGCAGGACTTCATTATTAAGACGGTCGAATTCGCCGTTCGCCCGCCAGATGTA                                     <  SRR4302535.638204/94‑1 (MQ=60)
                                                          GCAAGCGCCGTCAGAACTCTTTTCATGATGCTTGTGCCTCATGCAGGACTTCATTATTAAGACGGTCGAATTCGCCGTTCGCCCGCCAGATGTA                                     >  SRR4302535.575271/1‑94 (MQ=60)
                                                           CAAGCGCCGTCAGAACTCTTTTCATGATGCTTGTGCCTCATGCAGGACTTCATTATTAAGACGGTCGAATTCGCCGTTCGCCCGCCAGATGTAG                                    >  SRR4302535.4535901/1‑94 (MQ=60)
                                                           CAAGCGCCGTCAGAACTCTTTTCATGATGCTTGTGCCTCATGCAGGACTTCATTATTAAGACGGTCGAATTCGCCGTTCGCCCGCCAGATGTAG                                    >  SRR4302535.308309/1‑94 (MQ=60)
                                                            AAGCGCCGTCAGAACTCTTTTCATGATGCTTGTGCCTCATGCAGGACTTCATTATTAAGACGGTCGAATTCGCCGTTCGCCCGCCAGATGTAGA                                   >  SRR4302535.2711012/1‑94 (MQ=60)
                                                            AAGCGCCGTCAGAACTCTCTTCATGATGCTTGTGCCTCATGCAGGACTTCATTATTAAGACGGTCGAATTCGCCGTTCGCCCGCCAGATGTAGA                                   <  SRR4302535.251398/94‑1 (MQ=60)
                                                            AAGCCCCGTCAGAACTCTTTTCATGATGCTTGTGCCTCATGCAGGACTTCATTATTAAGACGGTCGAATTCGCCGTTCGCCCGCCAGATGTAGA                                   <  SRR4302535.3641904/94‑1 (MQ=60)
                                                             AGCGCCGTCAGAACTCTTTTCATGATGCTTGTGCCTCATGCAGGACTTCATTATTAAGACGGTCGAATTCGCCGTTCGCCCGCCAGATGTAGAT                                  <  SRR4302535.2772826/94‑1 (MQ=60)
                                                              GCGCCGTCAGAACTCTTTTCATGATGCTTGTGCCTCATGCAGGACTTCATTATTAAGACGGTCGAATTCGCCGTTCGCCCGCCAGATGTAGATA                                 <  SRR4302535.331818/94‑1 (MQ=60)
                                                               CGCCGTCAGAACTCTTTTCATGATGCTTGTGCCTCATGCAGGACTTCATTATTAAGACGGTCGAATTCGCCGTTCGCCCGCCAGATGTAGATAC                                >  SRR4302535.3702157/1‑94 (MQ=60)
                                                               CGCCGTCAGAACTCTTTTCATGATGCTTGTGCCTCATGCAGGACTTCATTATTAAGACGGTCGAATTCGCCGTTCGCCCGCCAGATGTAGATAC                                <  SRR4302535.646606/94‑1 (MQ=60)
                                                               CGCCGTCAGAACTCTTTTCATGATGCTTGTGCCTCATGCAGGACTTCATTATTAAGACGGTCGAATTCGCCGTTCGCCCGCCAGATGTAGATAC                                <  SRR4302535.4465203/94‑1 (MQ=60)
                                                               CGCCGTCAGAACTCTTTTCATGATGCTTGTGCCTCATGCAGGACTTCATTATTAAGACGGTCGAATTCGCCGTTCGCCCGCCAGATGTAGATAC                                >  SRR4302535.1043260/1‑94 (MQ=60)
                                                               CGCCGTCAGAACTCTTTTCATGATGCTTGTGCCTCATGCAGGACTTCATTATTAAGACGGTCGAATTCGCCGTTCGCCCGCCAGATGTAGATAC                                <  SRR4302535.2496901/94‑1 (MQ=60)
                                                                GCCGTCAGAACTCTTTTCATGATGCTTGTGCCTCATGCAGGACTTCATTATTAAGACGGTCGAATTCGCCGTTCGCCCGCCAGATGTAGATACC                               >  SRR4302535.4272891/1‑94 (MQ=60)
                                                                 CCGTCAGAACTCTTTTCATGATGCTTGTGCCTCATGCAGGACTTCATTATTAAGACGGTCGAATTCGCCGTTCGCCCGCCAGATGTAGATACCG                              >  SRR4302535.3299857/1‑94 (MQ=60)
                                                                  CGTCAGAACTCTTTTCATGATGCTTGTGCCTCATGCAGGACTTCATTATTAAGACGGTCGAATTCGCCGTTCGCCCGCCAGATGTAGATACCGG                             >  SRR4302535.3764771/1‑94 (MQ=60)
                                                                  CGTCAGAACTCTTTTCATGATGCTTGTGCCTCATGCAGGACTTCATTATTAAGACGGTCGAATTCGCCGTTCGCCCGCCAGATGTAGATACCGG                             >  SRR4302535.1809741/1‑94 (MQ=60)
                                                                   GTCAGAACTCTTTTCATGATGCTTGTGCCTCATGCAGGACTTCATTATTAAGACGGTCGAATTCGCCGTTCGCCCGCCAGATGTAGATACCGGT                            >  SRR4302535.631594/1‑94 (MQ=60)
                                                                   GTCAGAACTCTTTTCATGATGCTTGTGCCTCATGCAGGACTTCATTATTAAGACGGTCGAATTCGCCGTTCGCCCGCCAGATGTAGATACCGGT                            <  SRR4302535.732039/94‑1 (MQ=60)
                                                                   GTCAGAACTCTTTTCATGATGCTTGTGCCTCATGCAGGACTTCATTATTAAGACGGTCGAATTCGCCGTTCGCCCGCCAGATGTAGATACCGGT                            >  SRR4302535.319818/1‑94 (MQ=60)
                                                                   GTCAGAACTCTTTTCATGATGCTTGTGCCTCATGCAGGACTTCATTATTAAGACGGTCGAATTCGCCGTTCGCCCGCCAGATGTAGATACCGGT                            >  SRR4302535.2053515/1‑94 (MQ=60)
                                                                    TCAGAAGTCTTTTCATGATGCTTGTGCCTCATGCAGGACTTCATTATTAAGACGGTCGAATTCGCCGTTCGCCCGCCAGATGTAGATACCGGTG                           <  SRR4302535.88595/94‑1 (MQ=60)
                                                                    TCAGAACTCTTTTCATGATGCTTGTGCCTCATGCAGGACTTCATTATTAAGACGGTCGAATTCGCCGTTCGCCCGCCAGATGTAGATACCGGTG                           >  SRR4302535.330952/1‑94 (MQ=60)
                                                                    TCAGAACTCTTTTCATGATGCTTGTGCCTCATGCAGGACTTCATTATTAAGACGGTCGAATTCGCCGTTCGCCCGCCAGATGTAGATACCGGTG                           <  SRR4302535.1679127/94‑1 (MQ=60)
                                                                     CAGAACTTTTTTCATGATGCTTGTGCCTCATGCAGGACTTCATTATTAAGACGGTCGAATTCGCCGTTCGCCCGCCAGATGTAGATACCGGTGA                          >  SRR4302535.4110113/1‑94 (MQ=60)
                                                                     CAGAACTCTTTTCATGATGCTTGTGCCTCATGCAGGACTTCATTATTAAGACGGTCGAATTCGCCGTTCGCCCGCCAGATGTAGATACCGGTGA                          >  SRR4302535.1400215/1‑94 (MQ=60)
                                                                      AGACCTCTTTTCATGATGCTTGTGCCTCATGCAGGACTTCATTATTAAGACGGTCGAATTCGCCGTTCGCCCGCCAGATGTAGATACCGGTGAG                         <  SRR4302535.1078137/94‑1 (MQ=60)
                                                                      AGAACTCTTTTCATGATGCTTGTGCCTCATGCAGGACTTCATTATTAAGACGGTCGAATTCGCCGTTCGCCCGCCAGATGTAGATACCGGTGAG                         >  SRR4302535.1573530/1‑94 (MQ=60)
                                                                      AGAACTCTTTTCATGATGCTTGTGCCTCATGCAGGACTTCATTATTAAGACGGTCGAATTCGCCGTTCGCCCGCCAGATGTAGATACCGGTGAG                         >  SRR4302535.1504618/1‑94 (MQ=60)
                                                                      AGAACTCTTTTCATGATGCTTGTGCCTCATGCAGGACTTCATTATTAAGACGGTCGAATTCGCCGTTCGCCCGCCAGATGTAGATACCGGTGAG                         >  SRR4302535.4219064/1‑94 (MQ=60)
                                                                      AGAACTCTTTTCATGATGCTTGTGCCTCATGCAGGACTTCATTATTAAGACGGTCGAATTCGCCGTTCGCCCGCCAGATGTAGATACCGGTGAG                         <  SRR4302535.1953986/94‑1 (MQ=60)
                                                                      AGAACTCTTTTCATGATGCTTGTGCCTCATGCAGGACTTCATTATTAAGACGGTCGAATTCGCCGTTCGCCCGCCAGATGTAGATACCGGTGAG                         >  SRR4302535.2873979/1‑94 (MQ=60)
                                                                        AACTCTTTTCATGATGCTTGTGCCTCATGCAGGACTTCATTATTAAGACGGTCGAATTCGCCGTTCGCCCGCCAGATGTAGATACCGGTGAGAA                       >  SRR4302535.4438706/1‑94 (MQ=60)
                                                                        AACTCTTTTCATGATGCTTGTGCCTCATGCAGGACTTCATTATTAAGACGGTCGAATTCGCCGTTCGCCCGCCAGATGTAGATACCGGTGAGAA                       >  SRR4302535.4700825/1‑94 (MQ=60)
                                                                        AACTCTTTTCATGATGCTTGTGCCTCATGCAGGACTTCATTATTAAGACGGTCGAATTCGCCGTTCGCCCGCCAGATGTAGATACCGGTGAGAA                       >  SRR4302535.629017/1‑94 (MQ=60)
                                                                         ACTCTTTTCATGATGCTTGTGCCTCATGCAGGACTTCATTATTAAGACGGTCGAATTCGCCGTTCGCCCGCCAGATGTAGATACCGGTGAGAAC                      <  SRR4302535.2357096/94‑1 (MQ=60)
                                                                         ACTCTTTTCATGATGCTTGTGCCTCATGCAGGACTTCATTATTAAGACGGTCGAATTCGCCGTTCGCCCGCCAGATGTAGATACCGGTGAGAAC                      >  SRR4302535.2084383/1‑94 (MQ=60)
                                                                          CTCTTTTCATGATGCTTGTGCCTCATGCAGGACTTCATTATTAAGACGGTCGAATTCGCCGTTCGCCCGCCAGATGTAGATACCGGTGAGAACA                     >  SRR4302535.3436143/1‑94 (MQ=60)
                                                                          CTCTTTTCATGATGCTTGTGCCTCATGCAGGACTTCATTATTAAGACGGTCGAATTCGCCGTTCGCCCGCCAGATGTAGATACCGGTGAGAACA                     <  SRR4302535.4450130/94‑1 (MQ=60)
                                                                          CTCTTTTCATGATGCTTGTGCCTCATGCAGGACTTCATTATTAAGACGGTCGAATTCGCCGTTCGCCCGCCAGATGTAGATACCGGTGAGAACA                     >  SRR4302535.1290847/1‑94 (MQ=60)
                                                                           TCTTTTCATGATGCTTGTGCCTCATGCAGGACTTCATTATTAAGACGGTCGAATTCGCCGTTCGCCCGCCAGATGTAGATACCGGTGAGAACAA                    >  SRR4302535.1134873/1‑94 (MQ=60)
                                                                            CTTTTCATGATGCTTGTGCCTCATGCAGGACTTCATTATTAAGACGGTCGAATTCGCCGTTCGCCCGCCAGATGTAGATACCGGTGAGAACAAA                   >  SRR4302535.615116/1‑94 (MQ=60)
                                                                             TTTTCATGATGCTTGTGCCTCATGCAGGACTTCATTATTAAGACGGTCGAATTCGCCGTTCGCCCGCCAGATGTAGATACCGGTGAGAACAAAG                  >  SRR4302535.4719631/1‑94 (MQ=60)
                                                                             TTTTCATGATGCTTGTGCCTCATGCAGGACTTCATTATTAAGACGGTCGAATTCGCCGTTCGCCCGCCAGATGTAGATACCGGTGAGAACAAAG                  <  SRR4302535.1233682/94‑1 (MQ=60)
                                                                             TTTTCATGATGCTTGTGCCTCATGCAGGACTTCATTATTAAGACGGTCGAATTCGCCGTTCGCCCGCCAGATGTAGATACCGGTGAGAACAAAG                  <  SRR4302535.4683041/94‑1 (MQ=60)
                                                                              TTTCATGATGCTTGTGCCTCATGCAGGACTTCATTATTAAGACGGTCGAATTCGCCGTTCGCCCGCCAGATGTAGATACCGGTGAGAACAAAGG                 <  SRR4302535.4167359/94‑1 (MQ=60)
                                                                                TCATGATGCTTGTGCCTCATGCAGGACTTCATTATTAAGACGGTCGAATTCGCCGTTCGCCCGCCAGATGTAGATACCGGTGAGAACAAAGGAG               <  SRR4302535.4329315/94‑1 (MQ=60)
                                                                                 CATGATGCTTGTGCCTCATGCAGGACTTCATTATTAAGACGGTCGAATTCGCCGTTCGCCCGCCAGATGTAGATACCGGTGAGAACAAAGGAGA              >  SRR4302535.1990284/1‑94 (MQ=60)
                                                                                 CATGATGCTTGTGCCTCATGCAGGACTTCATTATTAAGACGGTCGAATTCGCCGTTCGCCCGCCAGATGTAGATACCGGTGAGAACAAAGGAGA              >  SRR4302535.3295869/1‑94 (MQ=60)
                                                                                 CATGATGCTTGTGCCTCATGCAGGACTTCATTATTAAGACGGTCGAATTCGCCGTTCGCCCGCCAGATGTAGATACCGGTGAGAACAAAGGAGA              <  SRR4302535.390098/94‑1 (MQ=60)
                                                                                  ATGATGCTTGTGCCTCATGCAGGACTTCATTATTAAGACGGTCGAATTCGCCGTTCGCCCGCCAGATGTAGATACCGGTGAGAACAAAGGAGAT             <  SRR4302535.3486137/94‑1 (MQ=60)
                                                                                   TGATGCTTGTGCCTCATGCAGGACTTCATTATTAAGACGGTCGAATTCGCCGTTCGCCCGCCAGATGTAGATACCGGTGAGAACAAAGGAGATC            <  SRR4302535.1971528/94‑1 (MQ=60)
                                                                                   TGATGCTTGTGCCTCATGCAGGACTTCATTATTAAGACGGTCGAATTCGCCGTTCGCCCGCCAGATGTAGATACCGGTGAGAACAAAGGAGATC            <  SRR4302535.740620/94‑1 (MQ=60)
                                                                                    GATGCTTGTGCCTCATGCAGGACTTCATTATTAAGACGGTCGAATTCGCCGTTCGCCCGCCAGATGTAGATACCGGTGAGAACAAAGGAGATCA           <  SRR4302535.1447827/94‑1 (MQ=60)
                                                                                     ATGCTTGTGCCTCATGCAGGACTTCATTATTAAGACGGTCGAATTCGCCGTTCGCCCGCCAGATGTAGATACCGGTGAGAACAAAGGAGATCAC          >  SRR4302535.196359/1‑94 (MQ=60)
                                                                                      TGCTTGTGCCTCATGCAGGACTTCATTATTAAGACGGTCGAATTCGCCGTTCGCCCGCCAGATGTAGATACCGGTGAGAACAAAGGAGATCACA         >  SRR4302535.2667184/1‑94 (MQ=60)
                                                                                       GCTTGTGCCTCATGCAGGACTTCATTATTAAGACGGTCGAATTCGCCGTTCGCCCGCCAGATGTAGATACCGGTGAGAACAAAGGAGATCACAA        <  SRR4302535.3566541/94‑1 (MQ=60)
                                                                                       GCTTGTGCCTCATGCAGGACTTCATTATTAAGACGGTCGAATTCGCCGTTCGCCCGCCAGATGTAGATACCGGTGAGAACAAAGGAGATCACAA        <  SRR4302535.161277/94‑1 (MQ=60)
                                                                                       GCTTGTGCCTCATGCAGGACTTCATTATTAAGACGGTCGAATTCGCCGTTCGCCCGCCAGATGTAGATACCGGTGAGAACAAAGGAGATCACAA        <  SRR4302535.1794151/94‑1 (MQ=60)
                                                                                        CTTGTGCCTCATGCAGGACTTCATTATTAAGACGGTCGAATTCGCCGTTCGCCCGCCAGATGTAGATACCGGTGAGAACAAAGGAGATCACAAT       <  SRR4302535.2739284/94‑1 (MQ=60)
                                                                                        CTTGTGCCTCATGCAGGACTTCATTATTAAGACGGTCGAATTCGCCGTTCGCCCGCCAGATGTAGATACCGGTGAGAACAAAGGAGATCACAAT       >  SRR4302535.4031582/1‑94 (MQ=60)
                                                                                        CTTGTGCCTCATGCAGGACTTCATTATTAAGACGGTCGAATTCGCCGTTCGCCCGCCAGATGTAGATACCGGTGAGAACAAAGGAGATCACAAT       >  SRR4302535.1663148/1‑94 (MQ=60)
                                                                                        CTTGTGCCTCATGCAGGACTTCATTATTAAGACGGTCGAATTCGCCGTTCGCCCGCCAGATGTAGATACCGGTGAGAACAAAGGAGATCACAAT       >  SRR4302535.4425975/1‑94 (MQ=60)
                                                                                         TTGTGCCTCATGCAGGACTTCATTATTAAGACGGTCGAATTCGCCGTTCGCCCGCCAGATGTAGATACCGGTGAGAACAAAGGAGATCACAATC      <  SRR4302535.3304203/94‑1 (MQ=60)
                                                                                         TTGTGCCTCATGCAGGACTTCATTATTAAGACGGTCGAATTCGCCGTTCGCCCGCCAGATGTAGATACCGGTGAGAACAAAGGAGATCACAATC      <  SRR4302535.2678320/94‑1 (MQ=60)
                                                                                            TGCCTCATGCAGGACTTCATTATTAAGACGGTCGAATTCGCCGTTCGCCCGCCAGATGTAGATACCGGTGAGAACAAAGGAGATCACAATCACT   >  SRR4302535.891864/1‑94 (MQ=60)
                                                                                            TGCCTCATGCAGGACTTCATTATTAAGACGGTCGAATTCGCCGTTCGCCCGCCAGATGTAGATACCGGTGAGAACAAAGGAGATCACAATCACT   >  SRR4302535.395207/1‑94 (MQ=60)
                                                                                            TGCCTCATGCAGGACTTCATTATTAAGACGGTCGAATTCGCCGTTCGCCCGCCAGATGTAGATACCGGTGAGAACAAAGGAGATCACAATCACT   <  SRR4302535.304179/94‑1 (MQ=60)
                                                                                             GCCTCATGCAGGACTTCATTATTAAGACGGTCGAATTCGCCGTTCGCCCGCCAGATGTAGATACCGGTGAGAACAAAGGAGATCACAATCACTC  >  SRR4302535.3456646/1‑94 (MQ=60)
                                                                                             GCCTCATGCAGGACTTCATTATTAAGACGGTCGAATTCGCCGTTCGCCCGCCAGATGTAGATACCGGTGAGAACAAAGGAGATCACAATCACTC  >  SRR4302535.1067287/1‑94 (MQ=60)
                                                                                             |                                                                                             
GCGCTCTACGGCCCCGCTAATAGCATCCGCGGCGTTAGCTGCGAAAGGGAGTGTGGCGGCAAGCGCCGTCAGAACTCTTTTCATGATGCTTGTACCTCATGCAGGACTTCATTATTAAGACGGTCGAATTCGCCGTTCGCCCGCCAGATGTAGATACCGGTGAGAACAAAGGAGATCACAATCACTC  >  NC_000913/4282398‑4282584

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑