Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I2 R1	1873	183.1	9376148	98.2%	9207377	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,363,501	A→G	I284T (ATC→ACC)	dcuA ←	anaerobic dicarboxylate transport

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,363,501	0	A	G	99.6%	699.0 / NA	222	I284T (ATC→ACC)	dcuA	anaerobic dicarboxylate transport
Reads supporting (aligned to +/- strand): ref base A (0/0); major base G (110/111); minor base T (0/1); total (110/112)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 226 total aligned reads displayed.

TTGTTGGAAACGAAAGTATCGCCCAGCCACGCAACACCCAGGATACAAATACAGGCGCTCATACCTGCTTTGAAGGTGCTGGAGTTGAGGATGTTGTCGGTATCGACTTTACAGATAACGGTGGTCAGAGTTGCAACGCTGAGCATGATGATCAGGATTGCGTTGGTGGTGTTCATCAGC  >  NC_000913/4363411‑4363590

ttgttgGAAACGAAAGCATCGCCCAGCCACGCAACACCCAGGATACAAATACAGGCGCTCATACCTGCTTTGAAGGTGCTGGAGTTGAGGGtg                                                                                         >  2:919792/1‑93 (MQ=255)

ttgttgGAAACGAAAGCATCGCCCAGCCACGCAACACCCAGGATACAAATACAGGCGCTCATACCTGCTTTGAAGGTGCTGGAGTTGAGGGtg                                                                                         >  2:2268430/1‑93 (MQ=255)

 tgttgGAAACGAAAGCATCGCCCAGCCACGCAACACCCAGGATACAAATACAGGCGCTCATACCTGCTTTGAAGGTGCTGGAGTTGAGGgtgt                                                                                        <  2:3017598/93‑1 (MQ=255)

   ttgGAAACGAAAGCATCGCCCAGCCACGCAACACCCAGGATACAAATACAGGCGCTCATACCTGCTTTGAAGGTGCTGGAGTTGAGGGtgttg                                                                                      >  1:2553036/1‑93 (MQ=255)

   ttgGAAACGAAAGCATCGCCCAGCCACGCAACACCCAGGATACAAATACAGGCGCTCATACCTGCTTTGAAGGTGCTGGAGTTGAGGGtgttg                                                                                      >  1:2447869/1‑93 (MQ=255)

    tgGAAACGAAAGCATCGCCCAGCCACGCAACACCCAGGATACAAATACAGGCGCTCATACCTGCTTTGAAGGTGCTGGAGTTGAGGGtgttgt                                                                                     <  1:2647149/93‑1 (MQ=255)

     ggAAACGAAAGCATCGCCCAGCCACGCAACACCCAGGATACAAATACAGGCGCTCATACCTGCTTTGAAGGTGCTGGAGTTGAGGGTGTTGTc                                                                                    <  1:1105514/93‑1 (MQ=255)

     ggAAACGAAAGCATCGCCCAGCCACGCAACACCCAGGATACAAATACAGGCGCTCATACCTGCTTTGAAGGTGCTGGAGTTGAGGGTGTTGTc                                                                                    >  2:117402/1‑93 (MQ=255)

     ggAAACGAAAGCATCGCCCAGCCACGCAACACCCAGGATACAAATACAGGCGCTCATACCTGCTTTGAAGGTGCTGGAGTTGAGGGTGTTGTc                                                                                    <  2:175442/93‑1 (MQ=255)

      gAAACGAAAGCATCGCCCAGCCACGCAACACCCAGGATACAAATACAGGCGCTCATACCTGCTTTGAAGGTGCTGGAGTTGAGGGTGTTGTCg                                                                                   >  1:3151833/1‑93 (MQ=255)

      gAAACGAAAGCATCGCCCAGCCACGCAACACCCAGGATACAAATACAGGCGCTCATACCTGCTTTGAAGGTGCTGGAGTTGAGGGTGTTGTCg                                                                                   >  2:3476980/1‑93 (MQ=255)

       aaaCGAAAGCATCGCCCAGCCACGCAACACCCAGGATACAAATACAGGCGCTCATACCTGCTTTGAAGGTGCTGGAGTTGAGGGTGTTGTCgg                                                                                  <  2:4287385/93‑1 (MQ=255)

         aCGAAAGCATCGCCCAGCCACGCAACACCCAGGATACAAATACAGGCGCTCATACCTGCTTTGAAGGTGCTGGAGTTGAGGGTGTTGTCGGTa                                                                                <  1:4359191/93‑1 (MQ=255)

         aCGAAAGCATCGCCCAGCCACGCAACACCCAGGATACAAATACAGGCGCTCATACCTGCTTTGAAGGTGCTGGAGTTGAGGGGGTTGTCGGTa                                                                                >  2:2798421/1‑93 (MQ=255)

          cGAAAGCATCGCCCTGCCACGCAACACCCAGGATACAAATACAGGCGCTCATACCTGCTTTGAAGGTGCTGGAGTTGAGGGTGTTGTCGGTAt                                                                               >  2:2864705/1‑93 (MQ=255)

          cGAAAGCATCGCCCAGCCACGCAACACCCAGGATACAAATACAGGCGCTCATACCTGCTTTGAAGGTGCTGGAGTTGAGGGTGTTGTCGGTAt                                                                               >  1:2427029/1‑93 (MQ=255)

           gAAAGCATCGCCCAGCCACGCAACACCCAGGATACAAATACAGGCGCTCATACCTGCTTTGAAGGTGCTGGAGTTGAGGGTGTTGTCGGTATc                                                                              <  2:4028969/93‑1 (MQ=255)

           gAAAGCATCGCCCAGCCACGCAACACCCAGGATACAAATACAGGCGCTCATACCTGCTTTGAAGGTGCTGGAGTTGAGGGTGTTGTCGGTATc                                                                              >  1:4531401/1‑93 (MQ=255)

            aaaGCATCGCCCAGCCACGCAACACCCAGGATACAAATACAGGCGCTCATACCTGCTTTGAAGGTGCTGGAGTTGAGGGTGTTGTCTGTATCg                                                                             >  2:1043716/1‑93 (MQ=255)

            aaaGCATCGCCCAGCCACGCAACACCCAGGATACAAATACAGGCGCTCATACCTGCTTTGAAGGTGCTGGAGTTGAGGGTGTTGTCGGTATCg                                                                             >  2:1610909/1‑93 (MQ=255)

             aaGCATCGCCCAGCCACGCAACACCCAGGATACAAATACAGGCGCTCATACCTGCTTTGAAGGTGCTGGAGTTGAGGGTGTTGTCGGTATCGa                                                                            <  1:2111305/93‑1 (MQ=255)

              agcaTCGCCCAGCCACGCAACACCCAGGATACAAATACAGGCGCTCATACCTGCTTTGAAGGTGCTGGAGTTGAGGGTGTTGTCGGTATCGAc                                                                           <  1:4156680/90‑1 (MQ=255)

              agcaTCGCCCAGCCACGCAACACCCAGGATACAAATACAGGCGCTCATACCTGCTTTGAAGGTGCTGGAGTTGAGGGTGTTGTCGGTATCGAc                                                                           >  1:2336688/4‑93 (MQ=255)

                 aTCGCCCAGCCACGCAACACCCAGGATACAAATACAGGCGCTCATACCTGCTTTGAAGGTGCTGGAGTTGAGGGTGTTGTCGGTATCGACttt                                                                        <  2:1853543/93‑1 (MQ=255)

                 aTCGCCCAGCCACGCAACACCCAGGATACAAATACAGGCGCTCATACCTGCTTTGAAGGTGCTGGAGTTGAGGGTGTTGTCGGTATCGACttt                                                                        >  1:2217223/1‑93 (MQ=255)

                  tCGCCCAGCCACGCAACACCCAGGATACAAATACAGGCGCTCATACCTGCTTTGAAGGTGCTGGAGTTGAGGGTGTTGTCGGTATCGACTTTa                                                                       <  2:864653/93‑1 (MQ=255)

                   cGCCCAGCCACGCAACACCCAGGATACAAATACAGGCGCTCATACCTGCTTTGAAGGTGCTGGAGTTGAGGGTGTTGTCGGTATCGACTTTAc                                                                      <  1:3223107/93‑1 (MQ=255)

                    gCCCAGCCACGCAACACCCAGGATACAAATACAGGCGCTCATACCTGCTTTGAAGGTGCTGGAGTTGAGGGTGTTGTCGGTATCGACTTTACa                                                                     >  2:2449568/1‑93 (MQ=255)

                    gCCCAGCCACGCAACACCCAGGATACAAATACAGGCGCTCATACCTGCTTTGAAGGTGCTGGAGTTGAGGGTGTTGTCGGTATCGACTTTACa                                                                     <  1:2779777/93‑1 (MQ=255)

                    ccccAGCCACGCAGCACCCAGGATACAAATACAGGCGCTCATACCTGCTTTGAAGGTGCTGGAGTTGAGGGTGTTGTCGGTATCGACTTTACa                                                                     <  1:4110241/92‑1 (MQ=255)

                         gCCACGCAACACCCAGGATACAAATACAGGCGCTCATACCTGCTTTGAAGGTGCTGGAGTTGAGGGTGTTGTCGGTATCGACTTTACAGATaa                                                                <  2:4018427/93‑1 (MQ=255)

                           cACGCAACACCCAGGATACAAATACAGGCGCTCATACCTGCTTTGAAGGTGCTGGAGTTGAGGGTGTTGTCGGTATCGACTTTACAGATAACg                                                              <  1:2302728/93‑1 (MQ=255)

                           cACGCAACACCCAGGATACAAATACAGGCGCTCATACCTGCTTTGAAGGTGCTGGAGTTGAGGGTGTTGTCGGTATCGACTTTACAGATAACg                                                              <  2:2540801/93‑1 (MQ=255)

                            aCGCAACACCCAGGATACAAATACAGGCGCTCATACCTGCTTTGAAGGTGCTGGAGTTGAGGGTGTTGTCGGTATCGACTTTACAGATAACgg                                                             >  1:359074/1‑93 (MQ=255)

                            aCGCAACACCCAGGATACAAATACAGGCGCTCATACCTGCTTTGAAGGTGCTGGAGTTGAGGGTGTTGTCGGTATCGACTTTACAGATAACgg                                                             >  2:2973500/1‑93 (MQ=255)

                            aCGCAACACCCAGGATACAAATACAGGCGCTCATACCTGCTTTGAAGGTGCTGGAGTTGAGGGTGTTGTCGGTATCGACTTTACAGATAACgg                                                             <  1:4325727/93‑1 (MQ=255)

                             cGCAACACCCAGGATACAAATACAGGCGCTCATACCTGCTTTGAAGGTGGTGGAGTTGAGGGTGTTGTCGGTATCGACTTTACAGATAACggt                                                            <  1:4285751/93‑1 (MQ=255)

                             cGCAACACCCAGGATACAAATACAGGCGCTCATACCTGCTTTGAAGGTGCTGGAGTTGAGGGTGTTGTCGGTATCGACTTTACAGATAACggt                                                            <  2:3367977/93‑1 (MQ=255)

                             cGCAACACCCAGGATACAAATACAGGCGCTCATACCTGCTTTGAAGGTGCTGGAGTTGAGGGTGTTGTCGGTATCGACTTTACAGATAACggt                                                            <  1:1172553/93‑1 (MQ=255)

                              gCAACACCCAGGATACAAATACAGGCGCTCATACCTGCTTTGAAGGTGCTGGAGTTGAGGGTGTTGTCGGTATCGACTTTACAGATAACggtg                                                           <  2:675536/93‑1 (MQ=255)

                              gCAACACCCAGGATACAAATACAGGCGCTCATACCTGCTTTGAAGGTGCTGGAGTTGAGGGTGTTGTCGGTATCGACTTTACAGATAACggtg                                                           <  2:2286612/93‑1 (MQ=255)

                                aaCACCCAGGATACAAATACAGGCGCTCATACCTGCTTTGAAGGTGCTGGAGTTGAGGGTGTTGTCGGTATCGACTTTACAGATAACggtggt                                                         <  1:333053/93‑1 (MQ=255)

                                 acacCCAGGATACAAATACAGGCGCTCATACCTGCTTTGAAGGTGCTGGAGTTGAGGGTGTTGTCGGTATCGACTTTACAGATAACGGTGGTc                                                        >  2:1841236/1‑93 (MQ=255)

                                 acacCCAGGATACAAATACAGGCGCTCATACCTGCTTTGAAGGTGCTGGAGTTGAGGGTGTTGTCGGTATCGACTTTACAGATAACGGTGGTc                                                        >  1:2968931/1‑93 (MQ=255)

                                    cccAGGATACAAATACAGGCGCTCATACCTGCTTTGAAGGTGCTGGAGTTGAGGGTGTTGTCGGTATCGACTTTACAGATAACGGTGGTCaga                                                     <  1:562110/93‑1 (MQ=255)

                                    cccAGGATACAAATACAGGCGCTCATACCTGCTTTGAAGGTGCTGGAGTTGAGGGTGTTGTCGGTATCGACTTTACAGATAACGGTGGTCaga                                                     >  2:1386787/1‑93 (MQ=255)

                                    cccAGGATACAAATACAGGCGCTCATACCTGCTTTGAAGGTGCTGGAGTTGAGGGTGTTGTCGGTATCGACTTTACAGATAACGGTGGTCaga                                                     <  1:831813/93‑1 (MQ=255)

                                    cccAGGATACAAATACAGGCGCTCATACCTGCTTTGAAGGTGCTGGAGTTGAGGGTGTTGTCGGTATCGACTTTACAGATAACGGTGGTCaga                                                     <  1:3442335/93‑1 (MQ=255)

                                      cAGGATACAAATACAGGCGCTCATACCTGCTTTGAAGGTGCTGGAGTTGAGGGTGTTGTCGGTATCGACTTTACAGATAACGGTGGTCAGAGt                                                   >  1:1764388/1‑93 (MQ=255)

                                      cAGGATACAAATACAGGCGCTCATACCTGCTTTGAAGGTGCTGGAGTTGAGGGTGTTGTCGGTATCGACTTTACAGATAACGGTGGTCAGAGt                                                   >  2:3041293/1‑93 (MQ=255)

                                       aGGATACAAATACAGGCGCTCATACCTGCTTTGAAGGTGCTGGAGTTGAGGGTGTTGTCGGTATCGACTTTACAGATAACGGTGGTCAGAGtt                                                  >  1:514677/1‑93 (MQ=255)

                                       aGGATACAAATACAGGCGCTCATACCTGCTTTGAAGGTGCTGGAGTTGAGGGTGTTGTCGGTATCGACTTTACAGATAACGGTGGTCAGAGtt                                                  >  2:2375250/1‑93 (MQ=255)

                                        ggATACAAATACAGGCGCTCATACCTGCTTTGAAGGTGCTGGAGTTGAGGGTGTTGTCGGTATCGACTTTACAGATAACGGTGGTCAGAGTTg                                                 >  2:4098063/1‑93 (MQ=255)

                                        ggATACAAATACAGGCGCTCATACCTGCTTTGAAGGTGCTGGAGTTGAGGGTGTTGTCGGTATCGACTTTACAGATAACGGTGGTCAGAGTTg                                                 <  1:3266614/93‑1 (MQ=255)

                                        ggATACAAATACAGGCGCTCATACCTGCTTTGAAGGTGCTGGAGTTGAGGGTGTTGTCGGTATCGACTTTACAGATAACGGTGGTCAGAGTTg                                                 >  2:1626814/1‑93 (MQ=255)

                                         gATACAAATACAGGCGCTCATTCCTGCTTTGAAGGTGCTGGAGTTGAGGGTGTTGTCGGTATCGACTTTACAGATAACGGTGGTCAGAGTTGc                                                <  2:2497623/93‑1 (MQ=255)

                                         gATACAAATACAGGCGCTCATGCCTGCTTTGAAGGTGCTGGAGTTGAGGGTGTTGTCGGTATCGACTTTACAGATAACGGTGGTCAGAGTTGc                                                <  1:1629793/93‑1 (MQ=255)

                                           tACAAATACAGGCGCTCATACCTGCTTTGAAGGTGCTGGAGTTGAGGGTGTTGTCGGTATCGACTTTACAGATAACGGTGGTCAGAGTTGCaa                                              >  1:371611/1‑93 (MQ=255)

                                            aCAAATACAGGCGCTCATACCTGCTTTGAAGGTGCTGGAGTTGAGGGTGTTGTCGGTATCGACTTTACAGATAACGGTGGTCAGAGTTGCAAc                                             <  2:1342911/93‑1 (MQ=255)

                                             cAAATACAGGCGCTCATACCTGCTTAGAAGGTGCTGGAGTTGAGGGTGTTGTCGGTATCGACTTTACAGATAACGGTGGTCAGAGTTGCAACg                                            >  2:275163/1‑93 (MQ=255)

                                              aaaTACAGGCGCTCATACCTGCTTTGAAGGTGCTGGAGTTGAGGGTGTTGTCGGTATCGACTTTACAGATAACGGGGGTCAGAGTTGCAACGc                                           >  1:1398077/1‑93 (MQ=255)

                                                aTACAGGCGCTCATACCTGCTTTGAAGGTGCTGGAGTTGAGGGTGTTGTCGGTATCGACTTTACAGATAACGGTGGTCAGAGTTGCAACGCTg                                         >  2:3299815/1‑93 (MQ=255)

                                                 tACAGGCGCTCATACCTGCTTTGAAGGTGCTGGAGTTGAGGGTGTTGTCGGTATCGACTTTACAGATAACGGTGGTCAGAGTTGCAACGCTGa                                        <  1:4602079/93‑1 (MQ=255)

                                                    aGGCGCTCATACCTGCTTTGAAGGTGCTGGAGTTGAGGGTGTTGTCGGTATCGACTTTACAGATAACGGTGGTCAGAGTTGCAACGCTGAGCa                                     <  1:1465618/93‑1 (MQ=255)

                                                     ggCGCTCATACCTGCTTTGAAGGTGCTGGAGTTGAGGGTGTTGTCGGTATCGACTTTACAGATAACGGTGGTCAGAGTTGCAACGCTGAGCat                                    >  2:4514616/1‑93 (MQ=255)

                                                     ggCGCGCATACCTGCTTTGAAGGTGCTGGAGTTGAGGGTGTTGTCGGTATCGACTTTACAGATAACGGTGGTCAGAGTTGCAACGCTGAGCat                                    <  1:1001812/93‑1 (MQ=255)

                                                      gcgcTCATACCTGCTTTGAAGGTGCTGGAGTTGAGGGTGTTGTCGGTATCGACTTTACAGATAACGGTGGTCAGAGTTGCAACGCTGAGCatg                                   >  1:3520413/1‑93 (MQ=255)

                                                      gcgcTCATACCTGCTTTGAAGGTGCTGGAGTTGAGGGTGTTGTCGGTATCGACTTTACAGATAACGGTGGTCAGAGTTGCAACGCTGAGCatg                                   <  2:2571862/93‑1 (MQ=255)

                                                        gcTCATACCTGCTTTGAAGGTGCTGGAGTTGAGGGTGTTGTCGGTATCGACTTTACAGATAACGGTGGTCAGAGTTGCAACGCTGAGCatgat                                 <  1:1891540/93‑1 (MQ=255)

                                                         cTCATACCTGCTTTGAAGGTGCTGGAGTTGAGGGTGTTGTCGGTATCGACTTTACAGATAACGGTGGTCAGAGTTGCAACGCTGAGCatgatg                                <  1:3028672/93‑1 (MQ=255)

                                                           cATACCTGCTTTGAAGGTGCTGGAGTTGAGGGTGTTGTCGGTATCGACTTTACAGATAACGGTGGTCAGAGTTGCAACGCTGAGCATgatgat                              <  1:3413718/93‑1 (MQ=255)

                                                           cATACCTGCTTTGAAGGTGCTGGAGTTGAGGGTGTTGTCGGTATCGACTTTACAGATAACGGTGGGCAGAGTTGCAACGCTGAGCATgatgat                              >  1:1694818/1‑93 (MQ=255)

                                                            aTACCTGCTTTGAAGGTGCTGGAGTTGAGGGTGTTGTCGGTATCGACTTTACAGATAACGGTGGTCAGAGTTGCAACGCTGAGCATGATGATc                             <  1:1407005/93‑1 (MQ=255)

                                                             tACCTGCTTTGAAGGTGCTGGAGTTGAGGGTGTTGTCGGTATCGACTTTACAGATAACGGTGGTCAGAGTTGCAACGCTGAGCATGATGATCa                            <  1:1362087/93‑1 (MQ=255)

                                                             tACCTGCTTTGAAGGTGCTGGAGTTGAGGGTGTTGTCGGTATCGACTTTACAGATAACGGTGGTCAGAGTTGCAACGCTGAGCATGATGATCa                            <  1:380551/93‑1 (MQ=255)

                                                              aCCTGCTTTGAAGGTGCTGGAGTTGAGGGTGTTGTCGGTATCGACTTTACAGATAACGGTGGTCAGAGTTGCAACGCTGAGCATGATGATCAg                           <  1:2876197/93‑1 (MQ=255)

                                                              aCCTGCTTTGAAGGTGCTGGAGTTGAGGGTGTTGTCGGTATCGACTTTACAGATAACGGTGGTCAGAGTTGCAACGCTGAGCATGATGATCAg                           <  1:845095/93‑1 (MQ=255)

                                                               ccTGCTTTGAAGGTGCTGGAGTTGAGGGTGTTGTCGGTATCGACTTTACAGATAACGGTGGTCAGAGTTGCAACGCTGAGCATGATGATCAga                          >  1:3893565/1‑92 (MQ=255)

                                                                cTGCTTTGAAGGTGCTGGAGTTGAGGGTGTTGTCGGTATCGACTTTACAGATAACGGTGGTCAGAGTTGCAACGCTGAGCATGATGATCAGGa                         >  1:698236/1‑93 (MQ=255)

                                                                cTGCTTGGAAGGTGCTGGAGTTGAGGGTGTTGTCGGTATCGACTTTACAGATAACGGTGGTCAGAGTTGCAACGCTGAGCATGATGATCAGGa                         <  2:2243052/93‑1 (MQ=255)

                                                                 tGCTTTGAAGGTGCTGGAGTTGAGGGTGTTGTCGGTATCGACTTTACAGATAACGGTGGTCAGAGTTGCAACGCTGAGCATGATGATCAGGAt                        <  2:4345390/93‑1 (MQ=255)

                                                                  gCTTTGAAGGTGCTGGAGTTGAGGGTGTTGTCGGTATCGACTTTACAGATAACGGTGGTCAGAGTTGCAACGCTGAGCATGATGATCAGGAtt                       >  1:4465729/1‑93 (MQ=255)

                                                                  gCTTTGAAGGTGCTGGAGTTGAGGGTGTTGTCGGTATCGACTTTACAGATAACGGTGGTCAGAGTTGCAACGCTGAGCATGATGATCAGGAtt                       >  1:2405695/1‑93 (MQ=255)

                                                                  gCTTTGAAGGTGCTGGAGTTGAGGGTGTTGTCGGTATCGACTTTACAGATAACGGTGGTCAGAGTTGCAACGCTGAGCATGATGATCAGGAtt                       >  2:3754166/1‑93 (MQ=255)

                                                                  gCTTTGAAGGTGATGGAGTTGAGGGTGTTGTCGGTATCGACTTTACAGATAACGGTGGTCAGAGTTGCAACGCTGAGCATGATGATCAGGAtt                       <  2:1983495/93‑1 (MQ=255)

                                                                      tGAAGGTGCTGGAGTTGAGGGTGTTGTCGGTATCGACTTTACAGATAACGGTGGTCAGAGTTGCAACGCTGAGCATGATGATCAGGATTGCGt                   >  2:1903954/1‑93 (MQ=255)

                                                                       gAAGGTGCTGGAGTTGAGGGTGTTGTCGGTATCGACTTTACAGATAACGGTGGTCAGAGTTGCAACGCTGAGCATGATGATCAGGATTGCGtt                  <  2:3587572/93‑1 (MQ=255)

                                                                         aGGTGCTGGAGTTGAGGGTGTTGTCGGTATCGACTTTACAGATAACGGTGGTCAGAGTTGCAACGCTGAGCATGATGATCAGGATTGCGTtgg                <  1:2833984/93‑1 (MQ=255)

                                                                         aGGTGCTGGAGTTGAGGGTGTTGTCGGTATCGACTTTACAGATAACGGTGGTCAGAGTTGCAACGCTGAGCATGATGATCAGGATTGCGTtgg                >  1:2924259/1‑93 (MQ=255)

                                                                           gTGCTGGAGTTGAGGGTGTTGTCGGTATCGACTTTACAGATAACGGTGGTCAGAGTTGCAACGCTGAGCATGATGATCAGGATTGCGTtggtg              >  1:1059333/1‑93 (MQ=255)

                                                                             gCTGGAGTTGAGGGTGTTGTCGGTATCGACTTTACAGATAACGGTGGTCAGCGTTGCAATGCTGAGCATGATGATCAGGATTGCGTTggtggt            >  2:3156308/1‑93 (MQ=255)

                                                                               tGGAGTTGAGGGTGTTGTCGGTATCGACTTTACAGATAACGGTGGTCAGAGTTGCAACGCTGAGCATGATGATCAGGATTGCGTTGGTGgtgt          <  1:530772/93‑1 (MQ=255)

                                                                                ggAGTTGAGGGTGTTGTCGGTATCGACTTTACAGATAACGGTGGTCAGAGTTGCAACGCTGAGCATGATGATCAGGATTGCGTTGGTGGTGtt         <  1:4191708/93‑1 (MQ=255)

                                                                                  aGTTGAGGGTGTTGTCGGTATCGACTTTACAGATAACGGTGGTCAGAGTTGCAACGCTGAGCATGATGATCAGGATTGCGTTGGTGGTGTtca       >  1:1807996/1‑93 (MQ=255)

                                                                                  aGTTGAGGGTGTTGTCGGTATCGACTTTACAGATAACGGTGGTCAGAGTTGCAACGCTGAGCATGATGATCAGGATTGCGTTGGTGGTGTtca       <  2:2149649/93‑1 (MQ=255)

                                                                                   gTTGAGGGTGTTGTCGGTATCGACTTTACAGATAACGGTGGTCAGAGTTGCAACGCTGAGCATGATGATCAGGATTGCGTTGGTGGTGTtcat      >  2:2313621/1‑93 (MQ=255)

                                                                                      gAGGGTGTTGTCGGTATCGACTTTACAGATAACGGTGGTCAGAGTTGCAACGCTGAGCATGATGATCAGGATTGCGTTGGTGGTGTTCATCAg   <  2:2853609/93‑1 (MQ=255)

                                                                                      gAGGGTGTTGTCGGTATCGACTTTACAGATAACGGTGGTCAGAGTTGCAACGCTGAGCATGATGATCAGGATTGCGTTGGTGGTGTTCATCAg   <  2:2661436/93‑1 (MQ=255)

                                                                                       aGGGTGTTTTCGGTATCGATTTTACAGATAACGGTGGTCAGAGTTGCAACGCTGAGCATGATGATCAGGATTGCGTTGGTGGTGTTCATCAGc  >  2:4585730/1‑93 (MQ=255)

                                                                                       aGGGTGTTGTCGGTATCGACTTTACAGATAACGGTGGTCAGAGTTGCAACGCTGAGCATGATGATCAGGATTGCGTTGGTGGTGTTCATCAGc  <  2:1925541/93‑1 (MQ=255)

TTGTTGGAAACGAAAGTATCGCCCAGCCACGCAACACCCAGGATACAAATACAGGCGCTCATACCTGCTTTGAAGGTGCTGGAGTTGAGGATGTTGTCGGTATCGACTTTACAGATAACGGTGGTCAGAGTTGCAACGCTGAGCATGATGATCAGGATTGCGTTGGTGGTGTTCATCAGC  >  NC_000913/4363411‑4363590

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 20 ≤` `ATCG/ATCG` `< 28 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

ATGTTGTTGGAAACGAAAGTATCGCCCAGCCACGCAACACCCAGGATACAAATACAGGCGCTCATACCTGCTTTGAAGGTGCTGGAGTTGAGGATGTTGTCGGTATCGACTTTACAGATAACGGTGGTCAGAGTTGCAACGCTGAGCATGATGATCAGGATTGCGTTGGTGGTGTTCATCAGCGGTT  >  NC_000913/4363408‑4363594

ATGTTGTTGGAAACGAAAGCATCGCCCAGCCACGCAACACCCAGGATACAAATACAGGCGCTCATACCTGCTTTGAAGGTGCTGGAGTTGAGGG                                                                                               <  SRR4302535.2655171/94‑1 (MQ=60)

 TGTTGTTGGAAACGAAAGCATCGCCCAGCCACGCAACACCCAGGATACAAATACAGGCGCTCATACCTGCTTTGAAGGTGCTGGAGTTGAGGGT                                                                                              <  SRR4302535.4710060/94‑1 (MQ=60)

  GTTGTTGGAAACGAAAGCATCGCCCAGCCACGCAACACCCAGGATACAAATACAGGCGCTCATACCTGCTTTGAAGGTGCTGGAGTTGAGGGTG                                                                                             <  SRR4302535.1057146/94‑1 (MQ=60)

     GTTGGAAACGAAAGCATCGCCCAGCCACGCAACACCCAGGATACAAATACAGGCGCTCATACCTGCTTTGAAGGTGCTGGAGTTGAGGGTGTTG                                                                                          >  SRR4302535.2465505/1‑94 (MQ=60)

     GTTGGAAACGAAAGCATCGCCCAGCCACGCAACACCCAGGATACAAATACAGGCGCTCATACCTGCTTTGAAGGTGCTGGAGTTGAGGGTGTTG                                                                                          >  SRR4302535.2571417/1‑94 (MQ=60)

      TTGGAAACGAAAGCATCGCCCAGCCACGCAACACCCAGGATACAAATACAGGCGCTCATACCTGCTTTGAAGGTGCTGGAGTTGAGGGTGTTGT                                                                                         >  SRR4302535.3246094/1‑94 (MQ=60)

       TGGAAACGAAAGCATCGCCCAGCCACGCAACACCCAGGATACAAATACAGGCGCTCATACCTGCTTTGAAGGTGCTGGAGTTGAGGGTGTTGTG                                                                                        >  SRR4302535.3540025/1‑94 (MQ=60)

       TGGAAACGAAAGCATCGCCCAGCCACGCAACACCCAGGATACAAATACAGGCGCTCATACCTGCTTTGAAGGTGCTGGAGTTGAGGGTGTTGTC                                                                                        <  SRR4302535.2343354/94‑1 (MQ=60)

       TGGAAACGAAAGCATCGCCCAGCCACGCAACACCCAGGATACAAATACAGGCGCTCATACCTGCTTTGAAGGTGCTGGAGTTGAGGGTGTTGTC                                                                                        <  SRR4302535.251385/94‑1 (MQ=60)

       TGGAAACGAAAGCATCGCCCAGCCACGCAACACCCAGGATACAAATACAGGCGCTCATACCTGCTTTGAAGGTGCTGGAGTTGAGGGTGTTGTC                                                                                        <  SRR4302535.2520151/94‑1 (MQ=60)

       TGGAAACGAAAGCATCGCCCAGCCACGCAACACCCAGGATACAAATACAGGCGCTCATACCTGCTTTGAAGGTGCTGGAGTTGAGGGTGTTGTC                                                                                        <  SRR4302535.2666110/94‑1 (MQ=60)

        GGAAACGAAAGCATCGCCCAGCCACGCAACACCCAGGATACAAATACAGGCGCTCATACCTGCTTTGAAGGTGCTGGAGTTGAGGGTGTTGTCG                                                                                       >  SRR4302535.3173736/1‑94 (MQ=60)

        GGAAACGAAAGCATCGCCCAGCCACGCAACACCCAGGATACAAATACAGGCGCTCATACCTGCTTTGAAGGTGCTGGAGTTGAGGGTGTTGTCG                                                                                       <  SRR4302535.1113413/94‑1 (MQ=60)

         GAAACGAAAGCATCGCCCAGCCACGCAACACCCAGGATACAAATACAGGCGCTCATACCTGCTTTGAAGGTGCTGGAGTTGAGGGTGTTGTCGG                                                                                      >  SRR4302535.532933/1‑94 (MQ=60)

         GAAACGAAAGCAGCGCCCAGCCACGCAACACCCAGGATACAAATACAGGCGCTCATACCTGCTTTGAAGGTGCTGGAGTTGAGGGTGTTGTCGG                                                                                      <  SRR4302535.3552983/94‑1 (MQ=60)

          AAACGAAAGCATCGCCCAGCCACGCAACACCCAGGATACAAATACAGGCGCTCATACCTGCTTTGAAGGTGCTGGAGTTGAGGGTGTTGTCGGT                                                                                     >  SRR4302535.2438535/1‑94 (MQ=60)

            ACGAAAGCATCGCCCAGCCACGCAACACCCAGGATACAAATACAGGCGCTCATACCTGCTTTGAAGGTGCTGGAGTTGAGGGTGTTGTCGGTAT                                                                                   >  SRR4302535.2444518/1‑94 (MQ=60)

            ACGAAAGCATCGCCCAGCCACGCAACACCCAGGATACAAATACAGGCGCTCATACCTGCTTTGAAGGTGCTGGAGTTGAGGGTGTTGTCGGTAT                                                                                   >  SRR4302535.4312944/1‑94 (MQ=60)

            ACGAAAGCATCGCCCAGCCACGCAACACCCAGGATACAAATACAGGCGCTCATACCTGCTTTGAAGGTGCTGGAGTTGAGGGTGTTGTCGGTAT                                                                                   <  SRR4302535.4389030/94‑1 (MQ=60)

             CGAAAGCATCGCCCAGCGACGCAGCACCCAGGATACAAATACAGGCGCTCATGCCTGCTTTGAAGGTGCTGGAGTTGAGGGTGTTGTCGGTATC                                                                                  <  SRR4302535.141425/94‑1 (MQ=60)

             CGAAAGCATCGCCCAGCCACGCAACACCCAGGATACAAATACAGGCGCTCATACCTGCTTTGAAGGTGCTGGAGTTGAGGGTGTTGTCGGTATC                                                                                  >  SRR4302535.4562417/1‑94 (MQ=60)

              GAAAGCATCGCCCAGCCACGCAACACCCAGGATACAAATACAGGCGCTCATGCCTGCTTTGAAGGTGCTGGAGTTGAGGGTGTTGTCGGTATCG                                                                                 <  SRR4302535.2841689/94‑1 (MQ=60)

              GAAAGCATCGCCCAGCCACGCAACACCCAGGATACAAATACAGGCGCTCATACCTGCTTTGAAGGTGCTGGAGTTGAGGGTGTTGTCGGTATCG                                                                                 <  SRR4302535.1224316/94‑1 (MQ=60)

              GAAAGCATCGCCCAGCCACGCAACACCCAGGATACAAATACAGGCGCTCATACCTGCTTTGAAGGTGCTGGAGTTGAGGGTGTTGTCGGTATCG                                                                                 >  SRR4302535.3344487/1‑94 (MQ=60)

                AAGCATCGCCCAGCCACGCAACACCCAGGATACAAATACAGGCGCTCATACCTGCTTTGAAGGTGCTGGAGTTGAGGGTGTTGTCGGTATCGAC                                                                               >  SRR4302535.2353408/1‑94 (MQ=60)

                AAGCATCGCCCAGCCACGCAACACCCAGGATACAAATACAGGCGCTCATACCTGCTTTGAAGGTGCTGGAGTTGAGGGTGTTGTCGGTATCGAC                                                                               <  SRR4302535.3601582/94‑1 (MQ=60)

                AAGCATCGCCCAGCCACGCAACACCCAGGATACAAATACAGGCGCTCATACCTGCTTTGAAGGTGCTGGAGTTGAGGGTGTTGTCGGTATCGAC                                                                               <  SRR4302535.2126533/94‑1 (MQ=60)

                 AGCATCGCCCAGCCACGCAACACCCAGGATACAAATACAGGCGCTCATACCTGCTTTGAAGGTGCTGGAGTTGAGGGTGTTGTCGGTATCGACT                                                                              <  SRR4302535.4185133/94‑1 (MQ=60)

                 AGCATCGCCCAGCCACGCAACACCCAGGATACAAATACAGGCGCTCATACCTGCTTTGAAGGTGCTGGAGTTGAGGGTGTTGTCGGTATCGACT                                                                              >  SRR4302535.4366079/1‑94 (MQ=60)

                 AGCATCGCCCAGCCACGCAACACCCAGGATACAAATACAGGCGCTCATACCTGCTTTGAAGGTGCTGGAGTTGAGGGTGTTGTCGGTATCGACT                                                                              <  SRR4302535.240712/94‑1 (MQ=60)

                   CATCGCCCAGCCACGCAACACCCAGGATACAAATACAGGCGCTCATACCTGCTTTGAAGGTGCTGGAGTTGAGGGTGTTGTCGGTATCGACTTT                                                                            >  SRR4302535.2233004/1‑94 (MQ=60)

                    ATCGCCCAGCCACGCAACACCCAGGATACAAATACAGGCGCTCATACCTGCTTTGAAGGTGCTGGAGTTGAGGGTGTTGTCGGTATCGACTTTA                                                                           >  SRR4302535.1865208/1‑94 (MQ=60)

                    ATCGCCCAGCCACGCAACACCCAGGATACAAATACAGGCGCTCATACCTGCTTTGAAGGTGCTGGAGTTGAGGGTGTTGTCGGTATCGACTTTA                                                                           >  SRR4302535.2279314/1‑94 (MQ=60)

                    ATCGCCCAGCCACGCAACACCCAGGATACAAATACAGGCGCTCATACCTGCTTTGAAGGTGCTGGAGTTGAGGGTGTTGTCGGTATCGACTTTA                                                                           >  SRR4302535.553826/1‑94 (MQ=60)

                     TCGCCCAGCCACGCAACACCCAGGATACAAATACAGGCGCTCATACCTGCTTTGAAGGTGCTGGAGTTGAGGGTGTTGTCGGTATCGACTTTAC                                                                          <  SRR4302535.4588211/94‑1 (MQ=60)

                      CGCCCAGCCACGCAACACCCAGGATACAAATACAGGCGCTCATACCTGCTTTGAAGGTGCTGGAGTTGAGGGTGTTGTCGGTATCGACTTTACA                                                                         <  SRR4302535.3245450/94‑1 (MQ=60)

                       GCCCAGCCACGCAACACCCAGGATACAAATACAGGCGCTCATACCTGCTTTGAAGGTGCTGGAGTTGAGGGTGTTGTCGGTATCGACTTTACAG                                                                        <  SRR4302535.2799568/94‑1 (MQ=60)

                       CCCCAGCCACGCAGCACCCAGGATACAAATACAGGCGCTCATACCTGCTTTGAAGGTGCTGGAGTTGAGGGTGTTGTCGGTATCGACTTTACAG                                                                        <  SRR4302535.4138379/94‑1 (MQ=60)

                         CCAGCCACGCAACACCCAGGATACAAATACAGGCGCTCATACCTGCTTTGAAGGTGCTGGAGTTGAGGGTGTTGTCGGTATCGACTTTACAGAT                                                                      <  SRR4302535.3376241/94‑1 (MQ=60)

                           AGCCACGCAACACCCAGGATACAAATACAGGCGCTCATACCTGCTTTGAAGGTGCTGGAGTTGAGGGTGTTGTCGGTATCGACTTTACAGATAA                                                                    >  SRR4302535.412285/1‑94 (MQ=60)

                           AGCCACGCAACACCCAGGATACAAATACAGGCGCTCATACCTGCTTTGAAGGTGCTGGAGGTGAGGGTGTTGTCGGTATCGACTTTACAGATAA                                                                    >  SRR4302535.3828184/1‑94 (MQ=60)

                              CACGCAACACCCAGGATACAAATACAGGCGCTCATACCTGCTTTGAAGGTGCTGGAGTTGAGGGTGTTGTCGGTATCGACTTTACAGATAACGG                                                                 <  SRR4302535.2319204/94‑1 (MQ=60)

                              CACGCAACACCCAGGATACAAATACAGGCGCTCATACCTGCTTTGAAGGTGCTGGAGTTGAGGGTGTTGTCGGTATCGACTTTACAGATAACGG                                                                 >  SRR4302535.361561/1‑94 (MQ=60)

                              CACGCAACACCCAGGATACAAATACAGGCGCTCATACCTGCTTTGAAGGTGCTGGAGTTGAGGGTGTTGTCGGTATCGACTTTACAGATAACGG                                                                 <  SRR4302535.3347221/94‑1 (MQ=60)

                              CACGCAACACCCAGGATACAAATACAGGCGCTCATACCTGCTTTGAAGGTGCTGGAGTTGAGGGTGTTGTCGGTATCGACTTTACAGATAACGG                                                                 >  SRR4302535.864622/1‑94 (MQ=60)

                               ACGCAACACCCAGGATACAAATACAGGCGCTCATACCTGCTTTGAAGGTGCTGGAGTTGAGGGTGTTGTCGGTATCGACTTTACAGATAACGGT                                                                >  SRR4302535.85859/1‑94 (MQ=60)

                               ACGCAACACCCAGGATACAAATACAGGCGCTCATACCTGCTTTGAAGGTGCTGGAGTTGAGGGTGTTGTCGGTATCGACTTTACAGATAACGGT                                                                <  SRR4302535.4355308/94‑1 (MQ=60)

                                CGCAACACCCAGGATACAAATACAGGCGCTCATACCTGCTTTGAAGGTGGTGGAGTTGAGGGTGTTGTCGGTATCGACTTTACAGATAACGGTG                                                               <  SRR4302535.4315071/94‑1 (MQ=60)

                                CGCAACACCCAGGATACAAATACAGGCGCTCATACCTGCTTTGAAGGTGCTGGAGTTGAGGGTGTTGTCGGTATCGACTTTACAGATAACGGTG                                                               <  SRR4302535.1180926/94‑1 (MQ=60)

                                CGCAACACCCAGGATACAAATACAGGCGCTCATACCTGCTTTGAAGGTGCTGGAGTTGAGGGTGTTGTCGGTATCGACTTTACAGATAACGGTG                                                               >  SRR4302535.2336271/1‑94 (MQ=60)

                                CGCAACACCCAGGATACAAATACAGGCGCTCATACCTGCTTTGAAGGTGCTGGAGTTGAGGGTGTTGTCGGTATCGACTTTACAGATAACGGTG                                                               <  SRR4302535.4096121/94‑1 (MQ=60)

                                  CAACACCCAGGATACAAATACAGGCGCTCATACCTGCTTTGAAGGTGCTGGAGTTGAGGGTGTTGTCGGTATCGACTTTACAGATAACGGTGGT                                                             <  SRR4302535.846661/94‑1 (MQ=60)

                                   AACACCCAGGATACAAATACAGGCGCTCATACCTGCTTTGAAGGTGCTGGAGTTGAGGGTGTTGTCGGTATCGACTTTACAGATAACGGTGGTC                                                            >  SRR4302535.2989765/1‑94 (MQ=60)

                                   AACACCCAGGATACAAATACAGGCGCTCATACCTGCTTTGAAGGTGCTGGAGTTGAGGGTGTTGTCGGTATCGACTTTACAGATAACGGTGGTC                                                            <  SRR4302535.335359/94‑1 (MQ=60)

                                   AACACCCAGGATACAAATACAGGCGCTCATACCTGCTTTGAAGGTGCTGGAGTTGAGGGTGTTGTCGGTATCGACTTTACAGATAACGGTGGTC                                                            >  SRR4302535.4209104/1‑94 (MQ=60)

                                    ACACCCAGGATACAAATACAGGCGCTCATACCTGCTTTGAAGGTGCTGGAGTTGAGGGTGTTGTCGGTATCGACTTTACAGATAACGGTGGTCA                                                           <  SRR4302535.3866185/94‑1 (MQ=60)

                                      ACCCAGGATACAAATACAGGCGCTCATGCCTGCTTTGAAGGTGCTGGAGTTGAGGGTGTTGTCGGTATCGACTTTACAGATAACGGTGGTCAGA                                                         <  SRR4302535.537500/94‑1 (MQ=60)

                                       CCCAGGATACAAATACAGGCGCTCATACCTGCTTTGAAGGTGCTGGAGTTGAGGGTGTTGTCGGTATCGACTTTACAGATAACGGTGGTCAGAG                                                        <  SRR4302535.837642/94‑1 (MQ=60)

                                       CCCAGGATACAAATACAGGCGCTCATACCTGCTTTGAAGGTGCTGGAGTTGAGGGTGTTGTCGGTATCGACTTTACAGATAACGGTGGTCAGAG                                                        <  SRR4302535.566040/94‑1 (MQ=60)

                                       CCCAGGATACAAATACAGGCGCTCATACCTGCTTTGAAGGTGCTGGAGTTGAGGGTGTTGTCGGTATCGACTTTACAGATAACGGTGGTCAGAG                                                        >  SRR4302535.2713461/1‑94 (MQ=60)

                                       CCCAGGATACAAATACAGGCGCTCATACCTGCTTTGAAGGTGCTGGAGTTGAGGGTGTTGTCGGTATCGACTTTACAGATAACGGTGGTCAGAG                                                        >  SRR4302535.157012/1‑94 (MQ=60)

                                       CCCAGGATACAAATACAGGCGCTCATACCTGCTTTGAAGGTGCTGGAGTTGAGGGTGTTGTCGGTATCGACTTTACAGATAACGGTGGTCAGAG                                                        <  SRR4302535.3466273/94‑1 (MQ=60)

                                        CCAGGATACAAATACAGGCGCTCATACCTGCTTTGAAGGTGCTGGAGTTGAGGGTGTTGTCGGTATCGACTTTACAGATAACGGTGGTCAGAGT                                                       >  SRR4302535.1732068/1‑94 (MQ=60)

                                        CCAGGATACAAATACAGGCGCTCATACCTGCTTTGAAGGTGCTGGAGTTGAGGGTGTTGTCGGTATCGACTTTACAGATAACGGTGGTCAGAGT                                                       >  SRR4302535.1777175/1‑94 (MQ=60)

                                         CAGGATACAAATACAGGCGCTCATACCTGCTTTGAAGGTGCTGGAGTTGAGGGTGTTGTCGGTATCGACTTTACAGATAACGGTGGTCAGAGTT                                                      >  SRR4302535.518276/1‑94 (MQ=60)

                                           GGATACAAATACAGGCGCTCATACCTGCTTTGAAGGTGCTGGAGTTGAGGGTGTTGTCGGTATCGACTTTACAGATAACGGTGGTCAGAGTTGC                                                    >  SRR4302535.4315826/1‑94 (MQ=60)

                                           GGATACAAATACAGGCGCTCATACCTGCTTTGAAGGTGCTGGAGTTGAGGGTGTTGTCGGTATCGACTTTACAGATAACGGTGGTCAGAGTTGC                                                    <  SRR4302535.3289307/94‑1 (MQ=60)

                                            GATACAAATACAGGCGCTCATGCCTGCTTTGAAGGTGCTGGAGTTGAGGGTGTTGTCGGTATCGACTTTACAGATAACGGTGGTCAGAGTTGCA                                                   <  SRR4302535.1641563/94‑1 (MQ=60)

                                             ATACAAATACAGGCGCTCATACCTGCTTTGAAGGTGCTGGAGTTGAGGTTGTTGTCGGTATCGACTTTACAGATAACGGTGGTCAGAGTTGCAA                                                  <  SRR4302535.2446097/94‑1 (MQ=60)

                                             ATACAAATACAGGCGCTCATACCTGCTTTGAAGGTGCTGGAGTTGAGGGTGTTGTCGGTATCGACTTTACAGATAACGGTGGTCAGAGTTGCAA                                                  >  SRR4302535.374182/1‑94 (MQ=60)

                                              TACAAATACAGGCGCTCATACCTGCTTTGAAGGTGCTGGAGTTGAGGGTGTTGTCGGTATCGACTTTACAGATAACGGTGGTCAGAGTTGCAAC                                                 <  SRR4302535.3005162/94‑1 (MQ=60)

                                               ACAAATACAGGCGCTCATACCTGCTTTGAAGGTGCTGGAGTTGAGGGTGTTGTCGGTATCGACTTTACAGATAACGGTGGTCAGAGTTGCAACG                                                >  SRR4302535.1537195/1‑94 (MQ=60)

                                                CAAATACAGGCGCTCATACCTGCTTTGAAGGTGCTGGAGTTGAGGGTGTTGTCGGTATCGACTTTACAGATAACGGGGGTCAGAGTTGCAACGC                                               >  SRR4302535.1408058/1‑94 (MQ=60)

                                                 AAATACAGGCGCTCATACCTGCTTTGAAGGTGCTGGAGTTGAGGGTGTTGTCGGTATCGACTTTACAGATAACGGTGCTCAGAGTTGCAACGCT                                              >  SRR4302535.773412/1‑94 (MQ=60)

                                                  AATACAGGCGCTCATACCTGCTTTGAAGGTGCTGGAGTTGAGGGTGTTGTCGGTATCGACTTTACAGATAACGGTGGTCAGAGTTGCAACGCTG                                             <  SRR4302535.3051140/94‑1 (MQ=60)

                                                    TACAGGCGCTCATACCTGCTTTGAAGGTGCTGGAGTTGAGGGTGTTGTCGGTATCGACTTTACAGATAACGGTGGTCAGAGTTGCAACGCTGAG                                           <  SRR4302535.4633375/94‑1 (MQ=60)

                                                       AGGCGCTCATACCTGCTTTGAAGGTGCTGGAGTTGAGGGTGTTGTCGGTATCGACTTTACAGATAACGGTGGTCAGAGTTGCAACGCTGAGCAT                                        <  SRR4302535.1476119/94‑1 (MQ=60)

                                                        GGCGCTCATACCTGCTTTGAAGGTGCTGGAGTTGAGGGTGTTGTCGGTATCGACTTTACAGATAACGGTGGTCAGAGTTGCAACGCTGAGCATG                                       <  SRR4302535.1821678/94‑1 (MQ=60)

                                                        GGCGCTCATACCTGCTTTGAAGGTGCTGGAGTTGAGGGTGTTGTCGGTATCGACTTTACAGATAACGGTGGTCAGAGTTGCAACGCTGAGCATG                                       >  SRR4302535.3544864/1‑94 (MQ=60)

                                                        GGCGCGCATACCTGCTTTGAAGGTGCTGGAGTTGAGGGTGTTGTCGGTATCGACTTTACAGATAACGGTGGTCAGAGTTGCAACGCTGAGCATG                                       <  SRR4302535.1008930/94‑1 (MQ=60)

                                                          CGCTCATACCTGCTTTGAAGGTGCTGGAGTTGAGGGTGTTGTCGGTATCGACTTTACAGTTAACGGTGGTCAGAGTTGCAACGCTGAGCATGAT                                     >  SRR4302535.1205777/1‑94 (MQ=60)

                                                          CGCTCATACCTGCTTTGAAGGTGCTGGAGCTGAGGGTGTTGTCGGTATCGACTTTACAGATAACGGTGGTCAGAGTTGCAACGCTGAGCATGAT                                     >  SRR4302535.2876712/1‑94 (MQ=60)

                                                           GCTCATACCTGCTTTGAAGGTGCTGGAGTTGAGGGTGTTGTCGGTATCGACTTTACAGATAACGGTGGTCAGAGTTGCAACGCTGAGCATGATG                                    <  SRR4302535.1905161/94‑1 (MQ=60)

                                                            CTCATACCTGCTTTGAAGGTGCTGGAGTTGAGGGTGTTGTCGGTATCGACTTTACAGATAACGGTGGTCAGAGTTGCAACGCTGAGCATGATGA                                   <  SRR4302535.3049873/94‑1 (MQ=60)

                                                             TCATACCTGCTTTGAAGGTGCTGGAGTTGAGGGTGTTGTCGGTATCGACTTTACAGATAACGGTGGGCAGAGTTGCAACGCTGAGCATGATGAT                                  >  SRR4302535.1707071/1‑94 (MQ=60)

                                                              CATACCTGCTTTGAAGGTGCTGGAGTTGAGGGTGTTGTCGGTATCGACTTTACAGATAACGGTGGTCAGAGTTGCAACGCTGAGCATGATGATC                                 >  SRR4302535.2995774/1‑94 (MQ=60)

                                                              CATACCTGCTTTGAAGGTGCTGGAGTTGAGGGTGTTGTCGGTATCGACTTTACAGATAACGGTGGTCAGAGTTGCAACGCTGAGCATGATGATC                                 <  SRR4302535.3437469/94‑1 (MQ=60)

                                                               ATACCTGCTTTGAAGGTGCTGGAGTTGAGGGTGTTGTCGGTATCGACTTTACAGATAACGGTGGTCAGAGTTGCAACGCTGAGCATGATGATCA                                <  SRR4302535.1417058/94‑1 (MQ=60)

                                                               ATACCTGCTTTGAAGGTGCTGGAGTTGAGGGTGTTGTCGGTATCGACTTTACAGATAACGGTGGTCAGAGTTGCAACGCTGAGCATGATGATCA                                >  SRR4302535.3622194/1‑94 (MQ=60)

                                                               ATACCTGCTTTGAAGGTGCTGGAGTTGAGGGTGTTGTCGGTATCGACTTTACAGATAACGGTGGTCAGAGTTGCAACGCTGAGCATGATGATCA                                >  SRR4302535.2661531/1‑94 (MQ=60)

                                                                TACCTGCTTTGAAGGTGCTGGAGTTGAGGGTGTTGTCGGTATCGACTTTACAGATAACGGTGGTCAGAGTTGCAACGCTGAGCATGATGATCAG                               <  SRR4302535.383184/94‑1 (MQ=60)

                                                                TACCTGCTTTGAAGGTGCTGGAGTTGAGGGTGTTGTCGGTATCGACTTTACAGATAACGGTGGTCAGAGTTGCAACGCTGAGCATGATGATCAG                               <  SRR4302535.1371830/94‑1 (MQ=60)

                                                                 ACCTGCTTTGAAGGTGCTGGAGTTGAGGGTGTTGTCGGTATCGACTTTACAGATAACGGTGGTCAGAGTTGCAACGCTGAGCATGATGATCAGG                              <  SRR4302535.3442799/94‑1 (MQ=60)

                                                                 ACCTGCTTTGAAGGTGCTGGAGTTGAGGGTGTTGTCGGTATCGACTTTACAGATAACGGTGGTCAGAGTTGCAACGCTGAGCATGATGATCAGG                              <  SRR4302535.851027/94‑1 (MQ=60)

                                                                 ACCTGCTTTGAAGGTGCTGGAGTTGAGGGTGTTGTCGGTATCGACTTTACAGATAACGGTGGTCAGAGTTGCAACGCTGAGCATGATGATCAGG                              <  SRR4302535.2896571/94‑1 (MQ=60)

                                                                 ACCTGCTTTGAAGGTGCTGGAGTTGAGGGTGTTGTCGGTATCGACTTTACAGATAACGGTGGTCAGAGTTGCAACGCTGAGCATGATGATCAGA                              >  SRR4302535.3920253/1‑94 (MQ=60)

                                                                 ACCTGCTTTGAAGGTGCTGGAGTTGAGGGGGTTGTCGGTATCGACTTTACCGATAACGGTGGTCAGAGCTGCAACGCTTAGCATGATGATCAGG                              >  SRR4302535.1901372/1‑94 (MQ=60)

                                                                  CCTGCTTTGAAGGTGCTGGAGTTGAGGGTGTTGTCGGTATCGACTTTACAGATAACGGTGGTCAGAGTTGCAACGCTGAGCATGATGATCAGGA                             >  SRR4302535.703135/1‑94 (MQ=60)

                                                                  CCTGCTTTGAAGGTGCTGGAGTTGAGGGTGTTGTCGGTATCGACTTTACAGATAACGGTGGTCAGAGTTGCAACGCTGAGCATGATGATCAGGA                             >  SRR4302535.29326/1‑94 (MQ=60)

                                                                   CTGCTTTGAAGGTGCTGGAGTTGAGGGTGTTGTCGGTATCGACTTTACAGATAACGGTGGTCAGAGTTGCAACGCTGAGCATGATGATCAGGAT                            <  SRR4302535.4531340/94‑1 (MQ=60)

                                                                   CTGCTTTGAAGGTGCTGGAGTTGAGGGTGTTGTCGGTATCGACTTTACAGATAACGGTGGTCAGAGTTGCAACGCTGAGCATGATGATCAGGAT                            <  SRR4302535.1142255/94‑1 (MQ=60)

                                                                   CTGCTTTGAAGGTGCTGGAGTTGAGGGTGTTGTCGGTATCGACTTTACAGATAACGGTGGTCAGAGTTGCAACGCTGAGCATGATGATCAGGAT                            <  SRR4302535.4430432/94‑1 (MQ=60)

                                                                   CTGCTTTGAAGGTGCTGGAGTTGAGGGTGTTGTCGGTATCGACTTTACAGATAACGGTGGTCAGAGTTGCAACGCTGAGCATGATGATCAGGAT                            <  SRR4302535.3218038/94‑1 (MQ=60)

                                                                   CTGCTTTGAAGGTGCTGGAGTTGAGGGTGTTGTCGGTATCGACTTTACAGATAACGGTGGTCAGAGTTGCAACGCTGAGCATGATGATCAGGAT                            >  SRR4302535.2977277/1‑94 (MQ=60)

                                                                   CTGCTTTGAAGGTGCTGGAGTTGAGGGCGTTGTCGGTATCGACTTTACAGATAACGGTGGTCAGAGTTGCAACGCTGAGCATGATGATCAGGAT                            >  SRR4302535.2202335/1‑94 (MQ=60)

                                                                    TGCTTTGAAGGTGCTGGAGTTGAGGGTGTTGTCGGTATCGACTTTACAGATAACGGTGGTCAGAGTTGCAACGCTGAGCATGATGATCAGGATT                           >  SRR4302535.2423009/1‑94 (MQ=60)

                                                                    TGCTTTGAAGGTGCTGGAGTTGAGGGTGTTGTCGGTATCGACTTTACAGATAACGGTGGTCAGAGTTGCAACGCTGAGCATGATGATCAGGATT                           >  SRR4302535.4496360/1‑94 (MQ=60)

                                                                    TGCTTTGAAGGTGCTGGAGTTGAGGGTGTTGTCGGTATCGACTTTACAGATAACGGTGGTCAGAGTTGCAACGCTGAGCATGATGATCAGGATT                           <  SRR4302535.1922970/94‑1 (MQ=60)

                                                                    TGCTTTGAAGGTGCTGGAGTTGAGGGTGTTGTCGGTATCGACTTTACAGATAACGGTGGTCAGAGTTGCAACGCTGAGCATGATGATCAGGATT                           <  SRR4302535.4653101/94‑1 (MQ=60)

                                                                      CTTTGAAGGTGCTGGAGTTGAGGGTGTTGTCGGTATCGACTTTACAGATAACGGTGGTCAGAGTTGCAACGCTGAGCGTGATTATCAGGATTGG                         >  SRR4302535.1070240/1‑94 (MQ=60)

                                                                        TTGAAGGTGCTGGAGTTGAGGGTGTTGTCGGTATCGAGTTTACAGATAACGGTGGTCAGAGTTGCAACGCTGAGCATGATGATCAGGATTGCGT                       <  SRR4302535.1433601/94‑1 (MQ=60)

                                                                           AAGGTGCTGGAGTTGAGGGTGTTGTCGGTATCGACTTTACAGATAACGGTGGTCAGAGTTGCAACGCTGAGCATGATGATCAGGATTGCGTTGG                    >  SRR4302535.2944879/1‑94 (MQ=60)

                                                                           AAGGTGCTGGAGTTGAGGGTGTTGTCGGTATCGACTTTACAGATAACGGTGGTCAGAGTTGCAACGCTGAGCATGATGATCAGGATTGCGTTGG                    <  SRR4302535.1403567/94‑1 (MQ=60)

                                                                            AGGTGCTGGAGTTGAGGGTGTTGTCGGTATCGACTTTACAGATAACGGTGGTCAGAGTTGCAACGCTGAGCATGATGATCAGGATTGCGTTGGT                   <  SRR4302535.2854098/94‑1 (MQ=60)

                                                                             GGTGCTGGAGTTGAGGGTGTTGTCGGTATCGACTTTACAGATAACGGTGGTCAGAGTTGCAACGCTGAGCATGATGATCAGGATTGCGTTGGTG                  >  SRR4302535.1066878/1‑94 (MQ=60)

                                                                             GGTGCTGGAGTTGAGGGTGTTGTCGGTATCGACTTTACAGATAACGGTGGTCAGAGTTGCAACGCTGAGCATGATGATCAGGATTGCGTTGGTG                  <  SRR4302535.1792398/94‑1 (MQ=60)

                                                                              GTGCTGGAGTTGAGGGTGTTGTCGGTATCGACTTTACAGATAACGGTGGTCAGAGTTGCAACGCTGAGCATGATGATCAGGATTGCGTTGGTGG                 <  SRR4302535.4172731/94‑1 (MQ=60)

                                                                                  TGGAGTTGAGGGTGTTGTCGGTATCGACTTTACAGATAACGGTGGTCAGAGTTGCAACGCTGAGCATGATGATCAGGATTGCGTTGGTGGTGTT             <  SRR4302535.1659208/94‑1 (MQ=60)

                                                                                  TGGAGTTGAGGGTGTTGTCGGTATCGACTTTACAGATAACGGTGGTCAGAGTTGCAACGCTGAGCATGATGATCAGGATTGCGTTGGTGGTGTT             <  SRR4302535.534483/94‑1 (MQ=60)

                                                                                  TGGAGTTGAGGGTGTTGTCGGTATCGACTTTACAGATAACGGTGGTCAGAGTTGCAACGCTGAGCATGATGATCAGGATTGCGTTGGTGGTGTT             <  SRR4302535.4593811/94‑1 (MQ=60)

                                                                                   GGAGTTGAGGGTGTTGTCGGTATCGACTTTACAGATAACGGTGGTCAGAGTTGCAACGCTGAGCATGATGATCAGGATTGCGTTGGTGGTGTTC            <  SRR4302535.4220393/94‑1 (MQ=60)

                                                                                    GAGTTGAGGGTGTTGTCGGTATCGACTTTACAGATAACGGTGGTCAGAGTTGCAACGCTGAGCATGATGATCAGGATTGCGTTGGTGGTGTTCA           >  SRR4302535.1821082/1‑94 (MQ=60)

                                                                                      GTTGAGGGTGTTGTCGGTATCGACTTTACAGATAACGGTGGTCAGAGTTGCAACGCTGAGCATGATGATCAGGATTGCGTTGGTGGTGTTCATC         >  SRR4302535.691013/1‑94 (MQ=60)

                                                                                      GTTGAGGGTGTTGTCGGTATCGACTTTACAGATAACGGTGGTCAGAGTTGCAACGCTGAGCATGATGATCAGGATTGCGTTGGTGGTGTTCATC         >  SRR4302535.2855379/1‑94 (MQ=60)

                                                                                        TGAGGGTGTTGTCGGTATCGACTTTACAGATAACGGTGGTCAGAGTTGCAACGCTGAGCATGATGATCAGGATTGCGTTGGTGGTGTTCATCAG       <  SRR4302535.4351318/94‑1 (MQ=60)

                                                                                          AGGGTGTTGTCGGTATCGACTTTACAGATAACGGTGGTCAGAGTTGCAACGCTGAGCATGATGATCAGGATTGCGTTGGTGGTGTTCATCAGCG     <  SRR4302535.1018585/94‑1 (MQ=60)

                                                                                             GTGTTGTCGGTATCGACTTTACAGATAACGGTGGTCAGAGTTGCAACGCTGAGCATGATGATCAGGATTGCGTTGGTGGTGTTCATCAGCGGTT  >  SRR4302535.274392/1‑94 (MQ=60)

ATGTTGTTGGAAACGAAAGTATCGCCCAGCCACGCAACACCCAGGATACAAATACAGGCGCTCATACCTGCTTTGAAGGTGCTGGAGTTGAGGATGTTGTCGGTATCGACTTTACAGATAACGGTGGTCAGAGTTGCAACGCTGAGCATGATGATCAGGATTGCGTTGGTGGTGTTCATCAGCGGTT  >  NC_000913/4363408‑4363594

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 8 ≤` `ATCG/ATCG` `< 26 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`