Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I1 R1	1548	369.5	20095359	96.9%	19472402	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	723,954	G→A	G83G (GGC→GGT)	kdpC ←	KdpC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	723,954	0	G	A	99.2%	917.8 / ‑5.6	263	G83G (GGC→GGT)	kdpC	KdpC
Reads supporting (aligned to +/- strand): ref base G (0/2); new base A (153/108); total (153/110)
Fisher's exact test for biased strand distribution p-value = 1.74e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01

Only 100 of 276 total aligned reads displayed.

CTGGCATCCGGGTTAGCGGCCCGTAATGCAGCAACGCGTGCGGCTATTAGTTTATCCAGCTCAGGGTTACTGACCGCCAGATTGCTCCCGCCAGAAGCCTGTGGATTATAGGGCATTTCTGCCGTTGCCGACGGGCGACCATGAAAATAGCCGTTGCCGGTAAAATTCTGCCCGATTAA  >  NC_000913/723865‑724043

cTGGCATCCGGGTTAGCGGCCCGTAATGCAGCAACGCGTGCGGCTATTAGTTTATCCAGCTCAGGGTTACTGACCGCCAGATTGCTCccacca                                                                                        <  1:146497/93‑1 (MQ=255)

  ggCATCCGGGTTAGCGGCCCGTAATGCAGCAACGCGTGCGGCTATTAGTTTATCCAGCTCAGGGTTACTGACCGCCAGATTGCTCCCACCAGa                                                                                      >  2:6176856/1‑93 (MQ=255)

   gCATCCGGGTTAGCGGCCCGTAATGCAGCAACGCGTGCGGCTATTAGTTTATCCAGCTCAGGGTTACTGACCGCCAGATTGCTCCCACCAGaa                                                                                     <  2:4174909/93‑1 (MQ=255)

    cATCCGGGTTAGCGGCCCGTAATGCAGCAACGCGTGCGGCTATTAGTTTATCCAGCTCAGGGTTACTGACCGCCAGATTGCTCCCACCAGAAg                                                                                    >  2:9254832/1‑93 (MQ=255)

     aTCCGGGTTAGCGGCCCGTAATGCAGCAACGCGTGCGGCTATTAGTTTATCCAGCTCAGGGTTACTGACCGCCAGATTGCTCCCACCAGAAGc                                                                                   >  1:8260999/1‑93 (MQ=255)

      tCCGGGTTCGCGGCCAGTAATGCAGCAACGCGTGCGGCTATTAGTTTATCCACGTCAGGGTTAATGACCGCCAGATTGGTCCCGCCAGAAGcc                                                                                  >  2:2430095/1‑93 (MQ=255)

      tCCGGGTTAGCGGCCCGTAATGCAGCAACGCGTGCGGCTATTAGTTTATCCAGCTCAGGGTTACTGACCGCCAGATTGCTCCCACCAGAAGcc                                                                                  >  2:85192/1‑93 (MQ=255)

      tCCGGGTTAGCGGCCCGTAATGCAGCAACGCGTGCGGCTATTAGTTTATCCAGCTCAGGGTTACTGACCGCCAGATTGCTCCCACCAGAAGcc                                                                                  >  1:5292972/1‑93 (MQ=255)

        cGGGTTAGCGGCCCGTAATGCAGCAACGCGTGCGGCTTTTAGTTTATCCAGCTCAGGGTTACTGACCGCCAGATTGCTCCCAACAGAAGCCtt                                                                                >  2:6610213/1‑92 (MQ=255)

        cGGGTTAGCGGCCCGTAATGCAGCAACGCGTGCGGCTATTAGTTTATCCAGCTCAGGGTTACTGACCGCCAGATTGCTCCCACCAGAAGCCtg                                                                                >  1:522502/1‑93 (MQ=255)

        cGGGTTAGCGGCCCGTAATGCAGCAACGCGTGCGGCTATTAGTTTATCCAGCTCAGGGTTACTGACCGCCAGATTGCTCCCACCAGAAGCCtg                                                                                <  1:2357563/93‑1 (MQ=255)

          ggTTAGCGGCCCGTAATGCAGCAACGCGTGCGGCTATTAGTTTATCCAGCTCAGGGTTACTGACCGCCAGATTGCTCCCACCAGAAGCCtgtg                                                                              <  1:6039600/93‑1 (MQ=255)

          ggTTAGCGGCCCGTAATGCAGCAACGCGTGCGGCTATTAGTTTATCCAGCTCAGGGTTACTGACCGCCAGATTGCTCCCACCAGAAGCCtgtg                                                                              >  1:2835509/1‑93 (MQ=255)

           gTTAGCGGCCCGTAATGCAGCAACGCGTGCGGCTATTAGTTTATCCAGCTCAGGGTTACTGACCGCCAGATTGCTCCCACCAGAAGCCTGTgg                                                                             <  2:36276/93‑1 (MQ=255)

            ttAGGGGCCCGTAATGCGGCAACGCGTGTGGCTATTAGTTTATCCAGCTCAGGGTTACTGACCGCGAGATTGCTCCCACCAGAAGCCTGTGGa                                                                            <  1:6787908/93‑1 (MQ=255)

            ttAGCGGCACGTAATGCAGGAACGCGTGCGGCTATTAGTTTAGCCAGCTCAGGGTTACTGACCGCCAGATTGCTCCCGCCAGAAGCCTGTGGa                                                                            <  1:1103424/93‑1 (MQ=255)

             tAGCGGCCCGTAATGCAGCAACGCGTGCGGCTATTAGTTTATCCAGCTCAGGGTTACTGACCGCCAGATTGCTCCCACCAGAAGCCTGTGGAt                                                                           >  1:4423831/1‑93 (MQ=255)

                cGGCCCGTAATGCAGCAACGCGTGCGGCTATTAGTTTATCCAGCTCAGGGTTACTGACCGCCAGATTGCTCCCACAAGAAGCCTGTGGttttt                                                                        >  2:7475378/1‑91 (MQ=255)

                  gCCCGTAATGCAGCAACGCGTGCGGCTATTAGTTTATCCAGCTCAGGGTTACTGACCGCCAGATTGCTCCCACCAGAAGCCTGTGGATTATAg                                                                      >  2:5479796/1‑93 (MQ=255)

                    ccGTAATGCAGCAACGCGTGCGGCTATTAGTTTATCCAGCTCAGGGTTACTGACCGCCAGATTGCTCCCACCAGAAGCCTGTGGATTATAggg                                                                    <  1:702281/93‑1 (MQ=255)

                    ccGTAATGCAGCAACGCGTGCGGCTATTAGTTTATCCAGCTCAGGGTTACTGACCGCCAGATTGATCCCACCAGAAGCCTGTGGATTATAggg                                                                    >  1:2073957/1‑93 (MQ=255)

                       tAATGCAGCAACGCGTGCGGCTATTAGTTTATCCAGCTCAGGGTTACTGACCGCCAGATTGCTCCCACCAGAAGCCTGTGGATTATAGGGCAt                                                                 >  1:4710176/1‑93 (MQ=255)

                       tAATGCAGCAACGCGTGCGGCTATTAGTTTATCCAGCTCAGGGTTACTGACCGCCAGATTGCTCCCACCAGAAGCCTGTGGATTATAGGGCAt                                                                 >  2:262042/1‑93 (MQ=255)

                       tAATGCAGCAACGCGTGCGGCTATTAGTTTATCCAGCTCAGGGTTACTGACCGCCAGATTGCTCCCACCAGAAGCCTGTGGATTATAGGGCAt                                                                 <  1:5600143/93‑1 (MQ=255)

                         aTGCAGCAACGCGTGCGGCTATTAGTTTATCCAGCTCAGGGTTACTGACCGCCAGATTGCTCCCACCAGAAGCCTGTGGATTATAGGGCAttt                                                               <  2:2003320/93‑1 (MQ=255)

                          tGCAGCAACGCGTGCGGCTATTAGTTTATCCAGCTCAGGGTTACTGACCGCCAGATTGCTCCCACCAGAAGCCTGTGGATTATAGGGCATTTc                                                              >  1:8279959/1‑93 (MQ=255)

                          tGCAGCAACGCGTGCGGCTATTAGTTTATCCAGCTCAGGGTTACTGACCGCCAGATTGCTCCCACCAGAAGCCTGTGGATTATAGGGCATTTc                                                              <  2:1876357/93‑1 (MQ=255)

                          tGCAGCAACGCGTGCGGCTATTAGTTTATCCAGCTCAGGGTTACTGACCGCCAGATTGCTCCCACCAGAAGCCTGTGGATTATAGGGCATTTc                                                              >  2:1958750/1‑93 (MQ=255)

                          tGCAGCAACGCGTGCGGCTATTAGTTTATCCAGCTCAGGGTTACTGACCGCCAGATTGCTCCCACCAGAAGCCTGTGGATTATAGGGCATTTc                                                              >  1:7863866/1‑93 (MQ=255)

                            cagcaACGCGTGCGGCTATTAGTTTATCCAGCTCAGGGTTACTGACCGCCAGATTGCTCCCACCAGAAGCCTGTGGATTATAGGGCATTTCTg                                                            >  1:1920342/1‑93 (MQ=255)

                              gcaACGCGTGCGGCTATTAGTTTATCCAGCTCAGGGTTACTGACCGCCAGATTGCTCCCACCAGAAGCCTGTGGATTATAGGGCATTTCTGcc                                                          >  1:4259525/1‑93 (MQ=255)

                              gcaACGCGTGCGGCTATTAGTTTATCCAGCTCAGGGTTACTGACCGCCAGATTGCTCCCACCAGAAGCCTGTGGATTATAGGGCATTTCTGcc                                                          >  1:8062832/1‑93 (MQ=255)

                               caACGCGTGCGGCTATTAGTTTATCCAGCTCAGGGTTACTGACCGCCAGATTGCTCCCACCAGAAGCCTGTGGATTATAGGGCATTTCTGCCg                                                         <  1:9475001/93‑1 (MQ=255)

                                aaCGCGTGCGGCTATTAGTTTATCCAGCTCAGGGTTACTGACCGCCAGATTGCTCCCACCAGAAGCCTGTGGATTATAGGGCATTTCTGCCGt                                                        <  2:559891/93‑1 (MQ=255)

                                aaCGCGTGCGGCTATTAGTTTATCCAGCTCAGGGTTACTGACCGCCAGATTGCTCCCACCAGAAGCCTGTGGATTATAGGGCATTTCTGCCGt                                                        >  1:78671/1‑93 (MQ=255)

                                aaCGCGTGCGGCTATTAGTTTATCCAGCTCAGGGTTACTGACCGCCAGATTGCTCCCACCAGAAGCCTGTGGATTATAGGGCATTTCTGCCGt                                                        <  2:2297478/93‑1 (MQ=255)

                                aaCGCGTGCGGCTATTAGTTTATCCAGCTCAGGGTTACTGACCGCCAGATTGCTCCCACCAGAAGCCTGTGGATTATAGGGCATTTCTGCCGt                                                        <  2:6337437/93‑1 (MQ=255)

                                 aCGCGTGCGGCTATTAGTTTATCCAGCTCAGGGTTACTGACCGCCAGATTGCTCCCACCAGAAGCCTGTGGATTATAGGGCATTTCTGCCGtt                                                       >  1:1866393/1‑93 (MQ=255)

                                   gcgTGCGGCTATTAGTTTATCCAGCTCAGGGTTACTGACCGCCAGATTGCTCCCACCAGAAGCCTGTGGATTATAGGGCATTTCTGCCGTTGc                                                     >  2:5136078/1‑93 (MQ=255)

                                   gcgTGCGGCGATTAGTTTATCCAGCTCAGGGTTACTGACCGCCAGATTGCTCCCACCAGAAGCCTGTGGATTATAGGGCATTTCTGCCGTTGc                                                     <  2:5031546/93‑1 (MQ=255)

                                    cgTGCGGCTATTAGTTTATCCAGCTCAGGGTTACTGACCGCCAGATTGCTCCCACCAGAAGCCTGTGGATTATAGGGCATTTCTGCCGTTGcc                                                    >  1:9045891/1‑93 (MQ=255)

                                     gTGCGGCTATTAGTTTATCCAGCTCAGGGTTACTGACCGCCAGATTTCTCCCACCAGAAGCCTGTGGATTATAGGGCATTTCTGCCGTTGcag                                                   >  1:6447228/1‑91 (MQ=255)

                                     gTGCGGCTATTAGTTTATCCAGCTCAGGGTTACTGACCGCCAGATTGCTCCCACCAGAAGCCTGTGGATTATAGGGCATTTCTGCCGTTGCCg                                                   <  2:832129/93‑1 (MQ=255)

                                      tGCGGCTATTAGTTTATCCAGCTCAGGGTTACTGACCGCCAGATTGCTCCCACCAGAAGCCTGTGGATTGTAGGGCATTTCTGCCGTTGCCGa                                                  >  2:6939706/1‑93 (MQ=255)

                                      tGCGGCTATTAGTTTATCCAGCTCAGGGTTACTGACCGCCAGATTGCTCCCACCAGAAGCCTGTGGATTATAGGGCATTTCTGCCGTTGCCGa                                                  <  1:1609059/93‑1 (MQ=255)

                                        cGGCTATTAGTTTATCCAGCTCAGGGTTACTGACCGCCAGATTGCTCCCACCAGAAGCCTGTGGATTATAGGGCATTTCTGCCGTTGCCGACg                                                >  1:6020581/1‑93 (MQ=255)

                                        cGGCTATTAGTTTATCCAGCTCAGGGTTACTGACCGCCAGATTGCTCCCACCAGAAGCCTGTGGATTATAGGGCATTTCTGCCGTTGCCGACg                                                <  1:310171/93‑1 (MQ=255)

                                         ggCTATTAGTTTATCCAGCTCAGGGTTACTGACCGCCAGATTGCTCCCACCAGAAGCCTGTGGATTATAGGGCATTTCTGCCGTTGCCGACgg                                               >  2:1302224/1‑93 (MQ=255)

                                          gCTATTAGTTTATCCAGCTCAGGGTTACTGACCGCCAGATTGCTCCCACCAGAAGCCTGTGGATTATAGGGCATTTCTGCCGTTGCCGACggg                                              >  1:100808/1‑93 (MQ=255)

                                           cTATTAGTTTATCCGGCTCAGGGTTACTGACCGCCAGATTGCTCCCACCAGAAGCCTGTGGATTATAGGGCATTTCTGCCGTTGCCGACGGGc                                             <  1:6340291/93‑1 (MQ=255)

                                             aTTAGTTTATCCAGCTCAGGGTTACTGACCGCCAGATTGCTCCCACCAGAAGCCTGTGGATTATAGGGCATTTCTGCCGTTGCCGACGGGCGa                                           >  1:5135168/1‑93 (MQ=255)

                                             aTTAGTTTATCCAGCTCAGGGTTACTGACCGCCAGATTGCTCCCACCAGAAGCCTGTGGATTATAGGGCATTTCTGCCGTTGCCGACGGGCGa                                           >  1:3851771/1‑93 (MQ=255)

                                             aTTAGTTTATCCAGCTCAGGGTTACTGACCGCCAGATTGCTCCCACCAGAAGCCTGTGGATTATAGGGCATTTCTGCCGTTGCCGACGGGCGa                                           >  1:7974798/1‑93 (MQ=255)

                                             aTTAGTTTATCCAGCTCAGGGTTACTGACCGCCAGATTGCTCCCACCAGAAGCCTGTGGATTATAGGGCATTTCTGCCGTTGCCGACGGGCGa                                           >  2:3247031/1‑93 (MQ=255)

                                              ttAGTTTATCCAGCTCAGGGTTACTGACCGCCAGATTGCTCCCACCAGAAGCCTGTGGATTATAGGGCATTTCTGCCGTTGCCGACGGGCGAc                                          >  1:1211473/1‑93 (MQ=255)

                                              ttAGTTTATCCAGCTCAGGGTTACTGACCGCCAGATTGCTCCCACCAGAAGCCTGTGGATTATAGGGCATTTCTGCCGTTGCCGACGGGCGAc                                          >  2:2080612/1‑93 (MQ=255)

                                               tAGTTTATCCAGCTCAGGGTTACTGACCGCCAGATTGCTCCCACCAGAAGCCTGTGGATTATAGGGCATTTCTGCCGTTGCCGACGGGCGAcc                                         >  2:5402273/1‑93 (MQ=255)

                                                 gTTTATCCAGCTCAGGGTTACTGACCGCCAGATTGCTCCCACCAGAAGCCTGTGGATTATAGGGCATTTCTGCCGTTGCCGACGGGCGACCAt                                       <  1:8574118/93‑1 (MQ=255)

                                                  tttATCCAGCTCAGGGTTACTGACCGCCAGATTGCTCCCACCAGAAGCCTGTGGATTATAGGGCGTTTCTGCCGTTGCCGACGGGCGACCATg                                      >  2:9004873/1‑93 (MQ=255)

                                                    tATCCAGCTCAGGGTTACTGACCGCCAGATTGCTCCCACCAGAAGCCTGTGGATTATAGGGCATTTCTGCCGTTGCCGACGGGCGACCATGaa                                    >  2:3433161/1‑93 (MQ=255)

                                                     aTCCAGCTCAGGGTTACTGACCGCCAGATTGCTCCCACCAGAAGCCTGTGGATTATAGGGCATTTCTGCCGTTGCCGACGGGCGACCATGaaa                                   <  2:8706778/93‑1 (MQ=255)

                                                     aTCCAGCTCAGGGTTACTGACCGCCAGATTGCTCCCACCAGAAGCCTGTGGATTATAGGGCATTTCTGCCGTTGCCGACGGGCGACCATGaaa                                   <  2:7035983/93‑1 (MQ=255)

                                                      tCCAGCTCAGGGTTACTGACCGCCAGATTGCTCCCACCAGAAGCCTGTGGATTATAGGGCATTTCTGCCGTTGCCGACGGGCGACCATGaaaa                                  >  2:7157559/1‑93 (MQ=255)

                                                      tCCAGCTCAGGGTTACTGACCGCCAGATTGCTCCCACCAGAAGCCTGTGGATTATAGGGCATTTCTGCCGTTGCCGACGGGCGACCATGaaaa                                  <  1:3659937/93‑1 (MQ=255)

                                                       ccAGCTCAGGGTTACTGACCGCCAGATTGCTCCCACCAGAAGCCTGTGGATTATAGGGCATTTCTGCCGTTGCCGACGGGCGACCATGAAAAt                                 <  1:1316984/93‑1 (MQ=255)

                                                        catcTCAGGGTTACTGACCGCCAGATTGCTCCCACCAGAAGCCTGTGGATTATAGGGCATTTCTGCCGTTGCCGACGGGCGACCATGAAAATa                                <  1:9102656/90‑1 (MQ=255)

                                                        cAGCTCAGGGTTACTGACCGCCAGATTGCTCCCACCAGAAGCCTGTGGATTATAGGGCATTTCTGCCGTTGCCGACGGGCGACCATGAAAATa                                <  1:5877134/93‑1 (MQ=255)

                                                        cAGCTCAGGGTTACTGACCGCCAGATTGCTCCCACCAGAAGCCTGTGGATTATAGGGCATTTCTGCCGTTGCCGACGGGCGACCATGAAAATa                                <  1:3407515/93‑1 (MQ=255)

                                                         aGCTCAGGGTTACTGACCGCCAGATTGCTCCCACCAGAAGCCTGTGGATTATAGGGCATTTCTGCCGTTGCCGACGGGCGACCATGAAAATAg                               >  2:3971738/1‑93 (MQ=255)

                                                         aGCTCAGGGTTACTGACCGCCAGATTGCTCCCACCAGAAGCCTGTGGATTATAGGGCATTTCTGCCGTTGCCGACGGGCGACCATGAAAATAg                               >  1:2564410/1‑93 (MQ=255)

                                                         aGCTCAGGGTTACTGACCGCCAGATTGCTCCCACCAGAAGCCTGTGGATTATAGGGCATTTCTGCCGTTGCCGACGGGCGACCATGAAAATAg                               >  2:1739580/1‑93 (MQ=255)

                                                          gCTCAGGGTTACTGACCGCCAGATTGCTCCCACCAGAAGCCTGTGGATTATAGGGCATTTCTGCCGTTGCCGACGGGCGACCATGAAAATAGc                              >  2:120348/1‑93 (MQ=255)

                                                          gCTCAGGGTTACTGACCGCCAGATTGCTCCCACCAGAAGCCTGTGGATTATAGGGCATTTCTGCCGTTGCCGACGGGCGACCATGAAAATAGc                              <  2:9567029/93‑1 (MQ=255)

                                                            tCAGGGTTACTGACCGCCAGATTGCTCCCACCAGAAGCCTGTGGATTATAGGGCATTTCTGCCGTTGCCGACGGGCGACCATGAAAATAGCCg                            <  2:4854154/93‑1 (MQ=255)

                                                            tCAGGGTTACTGACCGCCAGATTGCTCCCACCAGAAGCCTGTGGATTATAGGGCATTTCTGCCGTTGCCGACGGGCGACCATGAAAATAGCCg                            >  1:3532459/1‑93 (MQ=255)

                                                            tCAGGGTTACTGACCGCCAGATTGCTCCCACCAGAAGCCTGTGGATTATAGGGCATTTCTGCCGTTGCCGACGGGCGACCATGAAAATAGCCg                            >  2:2901434/1‑93 (MQ=255)

                                                             cAGGGTTACTGACCGCCAGATTGCTCCCACCAGAAGCCTGTGGATTATAGGGCATTTCTGCCGTTGCCGACGGGCGACCATGAAAATAGCCGt                           <  2:599358/93‑1 (MQ=255)

                                                                ggTTACTGACCGCCAGATTGCTCCCACCAGAAGCCTGTGGATTATAGGGCATTTCTGCCGTTGCCGACGGGCGACCATGAAAATAGCCGTTGc                        >  2:3787049/1‑93 (MQ=255)

                                                                   tACTGACCGCCAGATTGCTCCCACCAGAAGCCTGTGGATTATAGGGCATTTCTGCCGTTGCCGACGGGCGACCATGAAAATAGCCGTTGCCgg                     <  1:1560053/93‑1 (MQ=255)

                                                                   tACTGACCGCCAGATTGCTCCCACCAGAAGCCTGTGGATTATAGGGCATTTCTGCCGTTGCCGACGGGCGACCATGAAAATAGCCGTTGCCgg                     >  1:1697046/1‑93 (MQ=255)

                                                                    aCTGACCGCCAGATTGCTCCCACCAGAAGCCTGTGGATTATAGGGCATTTCTGCCGTTGCCGACGGGCGACCATGAAAATAGCCGTTGCCGGt                    >  2:1552338/1‑93 (MQ=255)

                                                                      tGACCGCCAGATTGCTCCCACCAGAAGCCTGTGGATTATAGGGCATTTCTGCCGTTGCCGACGGGCGACCATGAAAATAGCCGTTGCCGGTaa                  >  1:7483921/1‑93 (MQ=255)

                                                                        aCCGCCAGATTGCTCCCACCAGAAGCCTGTGGATTATAGGGCATTTCTGCCGTTGCCGACGGGCGACCATGAAAATAGCCGTTGCCGGTaaaa                <  2:8474904/93‑1 (MQ=255)

                                                                        aCCGCCAGATTGCTCCCACCAGAAGCCTGTGGATTATAGGGCATTTCTGCCGTTGCCGACGGGCGACAATGAAAATAGCCGTTGCCGGTaaaa                <  2:8831948/93‑1 (MQ=255)

                                                                          cGCCAGATTGCTCCCACCAGAAGCCTGTGGATTATAGGGCATTTCTGCCGTTGCCGACGGGCGACCATGAAAATAGCCGTTGCCGGTAAAAtt              <  2:4320886/93‑1 (MQ=255)

                                                                           gCCAGATTGCTCCCACCAGAAGCCTGTGGATTATAGGGCATTTCTGCCGTTGCCGACGGGCGACCATGAAAATAGCCGTTGCCGGTAAAATTc             >  1:4931442/1‑93 (MQ=255)

                                                                           gCCAGATTGCTCCCACCAGAAGCCTGTGGATTATAGGGCATTTCTGCCGTTGCCGACGGGCGACCATGAAAATAGCCGTTGCCGGTAAAATTc             >  1:88917/1‑93 (MQ=255)

                                                                            ccAGATTGCTCCCACCAGAAGCCTGTGGATTATAGGGCATTTCTGCCGTTGCCGACGGGCGACCATGAAAATAGCCGTTGCCGGTAAAATTCt            >  1:7693103/1‑93 (MQ=255)

                                                                             cAGATTGCTCCCACCAGAAGCCTGTGGATTATAGGGCATTTCTGCCGTTGCCGACGGGCGACCATGAAAATAGCCGTTGCCGGTAAAATTCTg           >  2:9619125/1‑93 (MQ=255)

                                                                              aGATTGCTCCCACCAGAAGCCTGTGGATTATAGGGCATTTCTGCCGTTGCCGACGGGCGACCATGAAAATAGCCGTTGCCGGTAAAATTCTGc          <  2:8159554/93‑1 (MQ=255)

                                                                              aGATTGCTCCCACCAGAAGCCTGTGGATTATAGGGCATTTCTGCCGTTGCCGACGGGCGACCATGAAAATAGCCGTTGCCGGTAAAATTCTGc          >  2:4973325/1‑93 (MQ=255)

                                                                              aGATTGCTCCCACCAGAAGCCTGTGGATTATAGGGCATTTCTGCCGTTGCCGACGGGCGACCATGAAAATAGCCGTTGCCGGTAAAATTCTGc          <  1:9139658/93‑1 (MQ=255)

                                                                               gATTGCTCCCACCAGAAGCCTGTGGATTATAGGGCATTTCTGCCGTTGCCGACGGGCGACCATGAAAATAGCCGTTGCCGGTAAAATTCTGcc         >  2:7848170/1‑93 (MQ=255)

                                                                               gATTGCTCCCACCAGAAGCCTGTGGATTATAGGGCATTTCTGCCGTTGCCGACGGGCGACCATGAAAATAGCCGTTGCCGGTAAAATTCTGcc         >  2:8071410/1‑93 (MQ=255)

                                                                                aTTGCCCCCACCAGAAGCCTGTGGATTATAGGGCATTTCTGCCGTTGCCGACGGGCGACCATGAAAATACCCGTTGCCGGTAAAATTCTGccc        <  2:4493918/93‑1 (MQ=255)

                                                                                 ttGCTCCCACCAGAAGCCTGTGGATTATAGGGCATTTCTGCCGTTGCCGACGGGCGACCATGAAAATAGCCGTTGCCGGTAAAATTCTGCCCg       >  2:6486324/1‑93 (MQ=255)

                                                                                    cTCCCACCAGAAGCCTGTGGATTATAGGGCATTTCTGCCGTTGCCGACGGGCGACCATGAAAATAGCCGTTGCCGGTAAAATTCTGCCCGAtt    >  1:2304439/1‑93 (MQ=255)

                                                                                     tCCCACCAGAAGCCTGTGGATTATAGGGCATTTCTGCCGTTGCCGACGGGCGACCATGAAAATAGCCGTTGCCGGTAAAATTCTGCCCGATTa   >  2:5314014/1‑93 (MQ=255)

                                                                                      cccACCAGAAGCCTGTGGATTATAGGGCATTTCTGCCGTTGCCGACGGGCGACCATGAAAATAGCCGTTGCCGGTAAAATTCTGCCCGATTaa  <  2:7396180/93‑1 (MQ=255)

                                                                                      cccACCAGAAGCCTGTGGATTATAGGGCATTTCTGCCGTCGCCGACGGGCGACCATGAAAATAGCCGTTGCCGGTAAAATTCTGCCCGATTaa  <  2:9854670/93‑1 (MQ=255)

CTGGCATCCGGGTTAGCGGCCCGTAATGCAGCAACGCGTGCGGCTATTAGTTTATCCAGCTCAGGGTTACTGACCGCCAGATTGCTCCCGCCAGAAGCCTGTGGATTATAGGGCATTTCTGCCGTTGCCGACGGGCGACCATGAAAATAGCCGTTGCCGGTAAAATTCTGCCCGATTAA  >  NC_000913/723865‑724043

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 19 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

CGCGCTGGCATCCGGGTTAGCGGCCCGTAATGCAGCAACGCGTGCGGCTATTAGTTTATCCAGCTCAGGGTTACTGACCGCCAGATTGCTCCCGCCAGAAGCCTGTGGATTATAGGGCATTTCTGCCGTTGCCGACGGGCGACCATGAAAATAGCCGTTGCCGGTAAAATTCTGCCCGATTAATG  >  NC_000913/723861‑724045

CGCGCTGGCATCCGGGTTAGCGGCCCGTAATGCAGCAACGCGTGCGGCTATTAGTTTATCCAGCTCAGGGTTACTGACCGCCAGATTGCTCCCA                                                                                             <  SRR4302540.5623717/94‑1 (MQ=60)

CGCGCTGGCATCCGGGTTAGCGGCCCGTAATGCAGCAACGCGTGCGGCTATTAGTTTATCCAGCTCAGGGTTACTGACCGCCAGATTGCTCCCA                                                                                             <  SRR4302540.5623718/94‑1 (MQ=60)

    CTGGCATCCGGGTTAGCGGCCCGTAATGCAGCAACGCGTGCGGCTATTAGTTTATCCAGCTCAGGGTTACTGACCGCCAGATTGCTCCCACCAG                                                                                         <  SRR4302540.148017/94‑1 (MQ=60)

    CTGGCATCCGGGTTAGCGGCCCGTAATGCAGCAACGCGTGCGGCTATTAGTTTATCCAGCTCAGGGTTACTGACCGCCAGATTGCTCCCACCAG                                                                                         <  SRR4302540.148016/94‑1 (MQ=60)

      GGCATCCGGGTTAGCGGCCCGTAATGCAGCAACGCGTGCGGCTATTAGTTTATCCAGCTCAGGGTTACTGACCGCCAGATTGCTCCCACCAGAA                                                                                       <  SRR4302540.8829328/94‑1 (MQ=60)

        CATCCGGGTTAGCGGCCCGTAATGCAGCAACGCGTGCGGCTATTAGTTTATCCAGCTCAGGGTTACTGACCGCCAGATTGCTCCCACCAGAAGC                                                                                     >  SRR4302540.8356999/1‑94 (MQ=60)

        CATCCGGGTTAGCGGCCCGTAATGCAGCAACGCGTGCGGCTATTAGTTTATCCAGCTCAGGGTTACTGACCGCCAGATTGCTCCCACCAGAAGC                                                                                     >  SRR4302540.8357001/1‑94 (MQ=60)

         ATCCGGGTTAGCGGCCCGTAATGCAGCAACGCGTGCGGCTATTAGTTTATCCAGCTCAGGGTTACTGACCGCCAGATTGCTCCCACCAGAAGCC                                                                                    >  SRR4302540.7720322/1‑94 (MQ=60)

         ATCCGGGTTAGCGGCCCGTAATGCAGCAACGCGTGCGGCTATTAGTTTATCCAGCTCAGGGTTACTGACCGCCAGATTGCTCCCACCAGAAGCC                                                                                    >  SRR4302540.5350847/1‑94 (MQ=60)

         ATCCGGGTTAGCGGCCCGTAATGCAGCAACGCGTGCGGCTATTAGTTTATCCAGCTCAGGGTTACTGACCGCCAGATTGCTCCCACCAGAAGCC                                                                                    >  SRR4302540.8109929/1‑94 (MQ=60)

           CCGGGTTAGCGGCCCGTAATGCAGCAACGCGTGCGGCTATTAGTTTATCCAGCTCAGGGTTACTGACCGCCAGATTGCTCCCACCAGAAGCCTG                                                                                  >  SRR4302540.528116/1‑94 (MQ=60)

            CGGGTTAGCGGCCCGTAATGCAGCAACGCGTGCGGCTATTAGTTTATCCAGCTCAGGGTTACTGACCGCCAGATTGCTCCCACCAGAAGCCTGT                                                                                 <  SRR4302540.2382957/94‑1 (MQ=60)

             GGGTTAGCGGCCCGTAATGCAGCAACGCGTGCGGCTATTAGTTTATCCAGCTCAGGGTTACTGACCGCCAGATTGCTCCCACCAGAAGCCTGTG                                                                                >  SRR4302540.2866176/1‑94 (MQ=60)

             GGGTTAGCGGCCCGTAATGCAGCAACGCGTGCGGCTATTAGTTTATCCAGCTCAGGGTTACTGACCGCCAGATTGCTCCCACCAGAAGCCTGTG                                                                                >  SRR4302540.9146687/1‑94 (MQ=60)

             GGGTTAGCGGCCCGTAATGCAGCAACGCGTGCGGCTATTAGTTTATCCAGCTCAGGGTTACTGACCGCCAGATTGCTCCCACCAGAAGCCTGTG                                                                                <  SRR4302540.3175456/94‑1 (MQ=60)

              GGTTAGCGGCCCGTAATGCAGCAACGCGTGCGGCTATTAGTTTATCCAGCTCAGGGTTACTGACCGCCAGATTGCTCCCACCAGAAGCCTGTGG                                                                               <  SRR4302540.6106664/94‑1 (MQ=60)

              GGTTAGCGGCCCGTAATGCAGCAACGCGTGCGGCTATTAGTTTATCCAGCTCAGGGTTACTGACCGCCAGATTGCTCCCACCAGAAGCCTGTGG                                                                               <  SRR4302540.479869/94‑1 (MQ=60)

               GTTAGCGGCCCGTAATGCAGCAACGCGTGCGGCTATTAGTTTATCCAGCTCAGGGTTACTGACCGCCAGATTGCTCCCACCAGAAGCCTGTGGA                                                                              <  SRR4302540.2195271/94‑1 (MQ=60)

                TTAGGGGCCCGTAATGCGGCAACGCGTGTGGCTATTAGTTTATCCAGCTCAGGGTTACTGACCGCGAGATTGCTCCCACCAGAAGCCTGTGGAT                                                                             <  SRR4302540.6862973/94‑1 (MQ=60)

                TTAGCGGCCCGTAATGCAGCAACGCGTGCGGCTATTAGTTTATCCAGCTCAGGGTTACTGACCGCCAGATTGCTCCCACCAGAAGCCTGTGGAT                                                                             <  SRR4302540.3657354/94‑1 (MQ=60)

                TTAGCGGCCCGTAATGCAGCAACGCGTGCGGCTATTAGTTTATCCAGCTCAGGGTTACTGACCGCCAGATTGCTCCCACCAGAAGCCTGTGGAT                                                                             >  SRR4302540.4471762/1‑94 (MQ=60)

                TTAGCGGCCCGTAATGCAGCAACGCGTGCGGCTATTAGTTTATCCAGCTCAGGGTTACTGACCGCCAGATTGCTCCCACCAGAAGCCTGTGGAT                                                                             <  SRR4302540.4337944/94‑1 (MQ=60)

                TTAGCGGCCCGTAATGCAGCAACGCGTGCGGCGATTAGTTTATCCAGCTCAGGCTTACTGACCGCCAGATTGCTCCCGCCAGAAGCCGGTGGAT                                                                             <  SRR4302540.6101388/94‑1 (MQ=60)

                TTAGCGGCACGTAATGCAGGAACGCGTGCGGCTATTAGTTTAGCCAGCTCAGGGTTACTGACCGCCAGATTGCTCCCGCCAGAAGCCTGTGGAT                                                                             <  SRR4302540.1114800/94‑1 (MQ=60)

                 TAGCGGCCCGTAATGCAGCAACGCGTGCGGCTATTAGTTTATCCAGCTCAGGGTTACTGACCGCCAGATTGCTCCCACCAGAAGCCTGTGGATT                                                                            <  SRR4302540.6192745/94‑1 (MQ=60)

                  AGCGGCCCGTAATGCAGCAACGCGTGCGGCTATTAGTTTATCCAGCTCAGGGTTACTGACCGCCAGATTGCTCCCACCAGAAGCCTGTGGATTA                                                                           <  SRR4302540.3929646/94‑1 (MQ=60)

                   GCGGCCCGTAATGCAGCAACGCGTGCGGCTATTAGTTTATCCAGCTCAGGGTTACTGACCGCCAGATTGCTCCCACCAGAAGCCTGTGGATTAT                                                                          <  SRR4302540.9214143/94‑1 (MQ=60)

                    CGGCCCGTAATGCAGCAACGCGTGCGTCTATTAGTTTATCCAGCTCAGGGTTACTGACCGCCAGATTGCTCCCACCAGAAGCCTGTGGATTATA                                                                         <  SRR4302540.6653171/94‑1 (MQ=60)

                    CGGCCCGTAATGCAGCAACGCGTGCGGCTATTAGTTTATCCAGCTCAGGGTTACTGACCGCCAGATTGCTCCCACCAGAAGCCTGTGGATTATA                                                                         <  SRR4302540.2354374/94‑1 (MQ=60)

                      GCTCGTAATGCAGCAACGCGTGCGGCTATTAGTTTATCCAGCTCAGGGTTACTGACCGCCAGATTGCTCCCACCAGAAGCCTGTGGATTATAGG                                                                       <  SRR4302540.2002970/94‑1 (MQ=60)

                      GCCCGTAATGCAGCAACGCGTGCGGCTATTAGTTTATCCAGCTCAGGGTTACTGACCGCCAGATTGATCCCANCAGAAGCCTGTGGATTATAGG                                                                       >  SRR4302540.1856310/1‑94 (MQ=60)

                       CCCGTAATGCAGCAACGCGTGCGGCTATTAGTTTATCCAGCTCAGGGTTACTGACCGCCAGATTGATCCCACCAGAAGCCTGTGGATTATAGGG                                                                      >  SRR4302540.2096258/1‑94 (MQ=60)

                        CCGTAATGCAGCAACGCGTGCGGCTATTAGTTTATCCAGCTCAGGGTTACTGACCGCCAGATTGCTCCCACCAGAAGCCTGTGGATTATAGGGC                                                                     <  SRR4302540.709817/94‑1 (MQ=60)

                         CGTAATGCAGCAACGCGTGCGGCTATTAGTTTATCCAGCTCAGGGTTACTGACCGCCAGATTGCTCCCACCAGAAGCCTGTGGATTATAGGGCA                                                                    >  SRR4302540.2473908/1‑94 (MQ=60)

                          GTAATGCAGCAACGCGTGCGGCTATTAGTTTATCCAGCTCAGGGTTACTGACCGCCAGATTGCTCCCACCAGAAGCCTGTGGATTATAGGGCAT                                                                   >  SRR4302540.4760515/1‑94 (MQ=60)

                          GTAATGCAGCAACGCGTGCGGCTATTAGTTTATCCAGCTCAGGGTTACTGACCGCCAGATTGCTCCCACCAGAAGCCTGTGGATTATAGGGCAT                                                                   <  SRR4302540.9134089/94‑1 (MQ=60)

                          GTAATGCAGCAACGCGTGCGGCTATTAGTTTATCCAGCTCAGGGTTACTGACCGCCAGATTGCTCCCACCAGAAGCCTGTGGATTATAGGGCAT                                                                   >  SRR4302540.8184073/1‑94 (MQ=60)

                           TAATGCAGCAACGTGTGCGGCTATTAGTTTATCCAGCTCAGGGTTACTGACCGCCAGATTGCTCCCACCAGAAGCCTGTGGATTATAGGGCATT                                                                  <  SRR4302540.3668390/94‑1 (MQ=60)

                           TAATGCAGCAACGCGTGCGGCTATTAGTTTATCCAGCTCAGGGTTACTGACCGCCAGATTGCTCCCACCAGAAGCCTGTGGATTATAGGGCATT                                                                  <  SRR4302540.1297085/94‑1 (MQ=60)

                           TAATGCAGCAACGCGTGCGGCTATTAGTTTATCCAGCTCAGGGTTACTGACCGCCAGATTGCTCCCACCAGAAGCCTGTGGATTATAGGGCATT                                                                  <  SRR4302540.5661754/94‑1 (MQ=60)

                            AATGCAGCAACGCGTGCGGCTATTAGTTTATCCAGCTCAGGGTTACTGACCGCCAGATTGCTCCCACCAGAAGCCTGTGGATTATAGGGCATTT                                                                 >  SRR4302540.5862179/1‑94 (MQ=60)

                             ATGCAGCAACGCGTGCGGCTATTAGTTTATCCAGCTCAGGGTTACTGACCGCCAGATTGCTCCCACCAGAAGCCTGTGGATTATAGGGCATTTC                                                                >  SRR4302540.7955737/1‑94 (MQ=60)

                             ATGCAGCAACGCGTGCGGCTATTAGTTTATCCAGCTCAGGGTTACTGACCGCCAGATTGCTCCCACCAGAAGCCTGTGGATTATAGGGCATTTC                                                                >  SRR4302540.8376126/1‑94 (MQ=60)

                             ATGCAGCAACGCGTGCGGCTATTAGTTTATCCAGCTCAGGGTTACTGACCGCCAGATTGCTCCCACCAGAAGCCTGTGGATTATAGGGCATTTC                                                                >  SRR4302540.1831131/1‑94 (MQ=60)

                             ATGCAGCAACGCGTGCGGCTATTAGTTTATCCAGCTCAGGGGTACTGACCGCCAGATTGCTCCCACCAGAAGCCTGTGGATTATAGGGCATTTC                                                                >  SRR4302540.8376128/1‑94 (MQ=60)

                              TGCAGCAACGCGTGCGGCTATTAGTTTATCCAGCTCAGGGTTACTGACCGCCAGATTGCTCCCACCAGAAGCCTGTGGATTATAGGGCATTTAT                                                               <  SRR4302540.1920638/94‑1 (MQ=60)

                               GCAGCAACGCGTGCGGCTATTAGTTTATCCAGCTCAGGGTTACTGACCGCCAGATTGCTCCCACCAGAAGCCTGTGGATTATAGGGCATTTCTG                                                              >  SRR4302540.1941291/1‑94 (MQ=60)

                                 AGCAACGCGTGCGGCTATTAGTTTATCCAGCTCAGGGTTACTGACCGCCAGATTGCTCCCACCAGAAGCCTGTGGATTATAGGGCATTTCTGCC                                                            >  SRR4302540.4305860/1‑94 (MQ=60)

                                 AGCAACGCGTGCGGCTATTAGTTTATCCAGCTCAGGGTTACTGACCGCCAGATTGCTCCCACCAGAAGCCTGTGGATTATAGGGCATTTCTGCC                                                            >  SRR4302540.8156413/1‑94 (MQ=60)

                                 AGCAACGCGTGCGGCTATTAGTTTATCCAGCTCAGGGTTACTGACCGCCAGATTGCTCCCACCAGAAGCCTGTGGATTATAGGGCATTTCTGCC                                                            >  SRR4302540.1350938/1‑94 (MQ=60)

                                   CAACGCGTGCGGCTATTAGTTTATCCAGCTCAGGGTTACTGACCGCCAGATTGCTCCCACCAGAAGCCTGTGGATTATAGGGCATTTCTGCCGT                                                          <  SRR4302540.9587373/94‑1 (MQ=60)

                                   CAACGCGTGCGGCTATTAGTTTATCCAGCTCAGGGTTACTGACCGCCAGATTGCTCCCACCAGAAGCCTGTGGATTATAGGGCATTTCTGCCGT                                                          >  SRR4302540.79497/1‑94 (MQ=60)

                                    AACGCGTGCGGCTATTAGTTTATCCAGCTCAGGGTTACTGACCGCCAGATTGCTCCCACCAGAAGCCTGTGGATTATAGGGCATTTCTGCCGTT                                                         >  SRR4302540.1886846/1‑94 (MQ=60)

                                    AACGCGTGCGGCTATTAGTTTATCCAGCTCAGGGTTACTGACCGCCAGATTGCTCCCACCAGAAGCCTGTGGATTATAGGGCATTTCTGCCGTT                                                         >  SRR4302540.8046452/1‑94 (MQ=60)

                                     ACGCGTGCGGCTATTAGTTTATCCAGCTCAGGGTTACTGACCGCCAGATTGCTCCCACCAGAAGCCTGTGGATTATAGGGCATTTCTGCCGTTG                                                        <  SRR4302540.710895/94‑1 (MQ=60)

                                     ACGCGTGCGGCTATTAGTTTATCCAGCTCAGGGTTACTGACCGCCAGATTGCTCCCACCAGAAGCCTGTGGATTATAGGGCATTTCTGCCGTTG                                                        >  SRR4302540.5790657/1‑94 (MQ=60)

                                     ACGCGTGCGGCTATTAGTTTATCCAGCTCAGGGTTACTGACCGCCAGATTGCTCCCACCAGAAGCCTGTGGATTATAGGGCATTTCTGCCGTTG                                                        <  SRR4302540.933614/94‑1 (MQ=60)

                                      CGCGTGCGGCTATTAGTTTATCCAGCTCAGGGTTACTGCCCGCCAGATTGCTCCCACCAGAAACCCTTGGATTATATGCCATTTCTGCCGgtgt                                                       >  SRR4302540.523412/1‑90 (MQ=60)

                                       GCGTGCGGCTATTAGTTTATCCAGCTCAGGGTTACTGACCGCCAGATTGCTCCCACCAGAAGCCTGTGGATTATAGGGCATTTCTGCCGTTGCC                                                      >  SRR4302540.3846621/1‑94 (MQ=60)

                                       GCGTGCGGCTATTAGTTTATCCAGCTCAGGGTTACTGACCGCCAGATTGCTCCCACCAGAAGCCTGTGGATTATAGGGCATTTCTGCCGTTGCC                                                      >  SRR4302540.9154475/1‑94 (MQ=60)

                                        CGTGCGGCTATTAGTTTATCCAGCTCAGGGTTACTGACCGCCAGATTTCTCCCACCAGAAGCCTGTGGATTATAGGGCATTTCTGCCGTTGCAG                                                     >  SRR4302540.6518996/1‑94 (MQ=60)

                                        CGTGCGGCTATTAGTTTATCCAGCTCAGGGTTACTGACCGCCAGATTGCTCCCACCAGAAGCCTGTGGATTATAGGGCATTTCTGCCGTTGCCG                                                     >  SRR4302540.2335247/1‑94 (MQ=60)

                                         GTGCGGCTATTAGTTTATCCAGCTCAGGGTTACTGACCGCCAGATTGCTCCCACCAGAAGCCTGTGGATTATAGGGCATTTCTGCCGTTGCCGA                                                    <  SRR4302540.3029665/94‑1 (MQ=60)

                                          TGCGGCTATTAGTTTATCCAGCTCAGGGTTACTGACCGCCAGATTGCTCCCACCAGAAGCCTGTGGATTATAGGGCATTTCTGCCGTTGCCGAC                                                   <  SRR4302540.1626675/94‑1 (MQ=60)

                                          TGCGGCTATTAGTTTATCCAGCTCAGGGTTACTGACCGCCAGATTGCTCCCACCAGAAGCCTGTGGATTATAGGGCATTTCTGCCGTTGCCGAC                                                   <  SRR4302540.1600848/94‑1 (MQ=60)

                                           GCGGCTATTAGTTTATCCAGCTCAGGGTTACTGACCGCCAGATTGCTCCCACCAGAAGCCTGTGGATTATAGGGCATTTCTGCCGTTGCCGACG                                                  >  SRR4302540.6087359/1‑94 (MQ=60)

                                           GCGGCTATTAGTTTATCCAGCTCAGGGTTACTGACCGCCAGATTGCTCCCACCAGAAGCCTGTGGATTATAGGGCATTTCTGCCGTTGCCGACG                                                  <  SRR4302540.9177954/94‑1 (MQ=60)

                                            CGGCTATTAGTTTATCCAGCTCAGGGTTACTGACCGCCAGATTGCTCCCACCAGAAGCCTGTGGATTATAGGGCATTTCTGCCGTTGCCGACGG                                                 <  SRR4302540.313246/94‑1 (MQ=60)

                                            CGGCTATTAGTTTATCCAGCTCAGGGTTACTGACCGCCAGATTGCTCCCACCAGAAGCCTGTGGATTATAGGGCATTTCTGCCGTTGCCGACGG                                                 >  SRR4302540.2738570/1‑94 (MQ=60)

                                            CGGCTATTAGTTTATCCAGCTCAGGGTTACTGACCGCCAGATTGCTCCCACCAGAAGCCTGTGGATTATAGGGCATTTCTGCCGTTGCCGACGG                                                 >  SRR4302540.6063890/1‑94 (MQ=60)

                                             GGCTATTAGTTTATCCAGCTCAGGGTTACTGACCGCCAGATTGCTCCCACCAGAAGCCTGTGGATTATAGGGCATTTCTGCCGTTGCCGACGGG                                                >  SRR4302540.101878/1‑94 (MQ=60)

                                             GGCTATTAGTTTATCCAGCTCAGGGTTACTGACCGCCAGATTGCTCCCACCAGAAGCCTGTGGATTATAGGGCATTTCTGCCGTTGCCGACGGG                                                >  SRR4302540.3334082/1‑94 (MQ=60)

                                             GGCTATTAGTTTATCCAGCTCAGGGTTACTGACCGCCAGATTGCTCCCACCAGAAGCCTGTGGATTATAGGGCATTTCTGCCGTTGCCGACGGG                                                <  SRR4302540.8490855/94‑1 (MQ=60)

                                               CTATTAGTTTATCCGGCTCAGGGTTACTGACCGCCAGATTGCTCCCACCAGAAGCCTGTGGATTATAGGGCATTTCTGCCGTTGCCGACGGGCG                                              <  SRR4302540.6410900/94‑1 (MQ=60)

                                               CTATTAGTTTATCCAGCTCAGGGTTACTGACCGCCAGATTGCTCCCACCAGAAGCCTGTGGATTATAGGGCATTTCTGCCGTTGCCGACGGGCG                                              <  SRR4302540.1700634/94‑1 (MQ=60)

                                               CTATTAGTTTATCCAGCTCAGGGTTACTGACCGCCAGATTGCTCCCACCAGAAGCCTGTGGATTATAGGGCATTTCTGCCGTTGCCGACGGGCG                                              >  SRR4302540.1704664/1‑94 (MQ=60)

                                               CTATTAGTTTATCCAGCTCAGGGTTACTGACCGCCAGATTGCTCCCACCAGAAGCCTGTGGATTATAGGGCATTTCTGCCGTTGCCGACGGGCG                                              >  SRR4302540.6455963/1‑94 (MQ=60)

                                               CTATTAGTTTATCCAGCTCAGGGTTACTGACCGCCAGATTGCTCCCACCAGAAGCCTGTGGATTATAGGGCATTTCTGCCGTTGCCGACGGGCG                                              <  SRR4302540.6410901/94‑1 (MQ=60)

                                               CTATTAGTTTATCCAGCTCAGGGTTACTGACCGCCAGATTGCTCCCACCAGAAGCCTGTGGATTATAGGGCATTTCTGCCGTTGCCGACGGGCG                                              <  SRR4302540.8022229/94‑1 (MQ=60)

                                                TATTAGTTTATCCAGCTCAGGGTTACTGACCGCCAGATTGCTCCCACCAGAAGCCTGTGGATTATAGGGCATTTCTGCCGTTGCCGACGGGCGA                                             >  SRR4302540.5114193/1‑94 (MQ=60)

                                                TATTAGTTTATCCAGCTCAGGGTTACTGACCGCCAGATTGCTCCCACCAGAAGCCTGTGGATTATAGGGCATTTCTGCCGTTGCCGACGGGCGA                                             >  SRR4302540.8067707/1‑94 (MQ=60)

                                                TATTAGTTTATCCAGCTCAGGGTTACTGACCGCCAGATTGCTCCCACCAGAAGCCTGTGGATTATAGGGCATTTCTGCCGTTGCCGACGGGCGA                                             >  SRR4302540.5190784/1‑94 (MQ=60)

                                                TATTAGTTTATCCAGCTCAGGGTTACTGACCGCCAGATTGCTCCCACCAGAAGCCTGTGGATTATAGGGCATTTCTGCCGTTGCCGACGGGCGA                                             >  SRR4302540.3893649/1‑94 (MQ=60)

                                                 ATTAGTTTATCCAGCTCAGGGTTACTGACCGCCAGATTGCTCCCACCAGAAGCCTGTGGATTATAGGGCATTTCTGCCGTTGCCGACGGGCGAC                                            >  SRR4302540.1223894/1‑94 (MQ=60)

                                                  TTAGTTTATCCAGCTCAGGGTTACTGACCGCCAGATTGCTCCCACCAGAAGCCTGTGGATTATAGGGCATTTCTGCCGTTGCCGACGGGCGACC                                           >  SRR4302540.8259952/1‑94 (MQ=60)

                                                  TTAGTTTATCCAGCTCAGGGTTACTGACCGCCAGATTGCTCCCACCAGAAGCCTGTGGATTATAGGGCATTTCTGCCGTTGCCGACGGGCGACC                                           <  SRR4302540.8142267/94‑1 (MQ=60)

                                                     GTTTATCCAGCTCAGGGTTACTGACCGCCAGATTGCTCCCACCAGAAGCCTGTGGATTATAGGGCATTTCTGCCGTTGCCGACGGGCGACCATG                                        >  SRR4302540.5344857/1‑94 (MQ=60)

                                                     GTTTATCCAGCTCAGGGTTACTGACCGCCAGATTGCTCCCACCAGAAGCCTGTGGATTATAGGGCATTTCTGCCGTTGCCGACGGGCGACCATG                                        <  SRR4302540.8673738/94‑1 (MQ=60)

                                                      TTTATCCAGCTCAGGGTTACTGACCGCCAGATTGCTCCCACCAGAAGCCTGTGGATTATAGGGCATTTCTGCCGTTGCCGACGGGCGACCATGA                                       >  SRR4302540.6407981/1‑94 (MQ=60)

                                                       TTATCCAGCTCAGGGTTACTGACCGCCAGATTGCTCCCACCGGAAGCCTGTGGATTATAGGGCATTTCTGCCGTTGCCGACGGGCGACCATGAA                                      <  SRR4302540.9428482/94‑1 (MQ=60)

                                                         ATCCAGCTCAGGGTTACTGACCGCCAGATTGCTCCCACCAGAAGCCTGTGGATTATAGGGCATTTCTGCCGTTGCCGACGGGCGACCATGAAAA                                    <  SRR4302540.3470483/94‑1 (MQ=60)

                                                         ATCCAGCTCAGGGTTACTGACCGCCAGATTGCTCCCACCAGAAGCCTGTGGATTATAGGGCATTTCTGCCGTTGCCGACGGGCGACCATGAAAA                                    >  SRR4302540.5028002/1‑94 (MQ=60)

                                                          TCCAGCTCAGGGTTACTGACCGCCAGATTGCTCCCACCAGAAGCCTGTGGATTATAGGGCATTTCTGCCGTTGCCGACGGGCGACCATGAAAAT                                   <  SRR4302540.2095484/94‑1 (MQ=60)

                                                          TCCAGCTCAGGGTTACTGACCGCCAGATTGCTCCCACCAGAAGCCTGTGGATTATAGGGCATTTCTGCCGTTGCCGACGGGCGACCATGAAAAT                                   <  SRR4302540.3700004/94‑1 (MQ=60)

                                                           CCAGCTCAGGGTTACTGACCGCCAGATTGCTCCCACCAGAAGCCTGTGGATTATAGGGCATTTCTGCCGTTGCCGACGGGCGACCATGAAAATA                                  <  SRR4302540.1330685/94‑1 (MQ=60)

                                                           CCAGCTCAGGGTTACTGACCGCCAGATTGCTCCCACCAGAAGCCTGTGGATTATAGGGCATTTCTGCCGTTGCCGACGGGCGACCATGAAAATA                                  <  SRR4302540.1330684/94‑1 (MQ=60)

                                                            cagctgagggtgacgcatCGCCAGATTGCTCGCACCAGAAGCCTGGTGATTATAGGGCATTTCTGCCGTGGCCGACGGGCGACCATGAAAATAG                                 <  SRR4302540.9212039/76‑1 (MQ=60)

                                                            CATCTCAGGGTTACTGACCGCCAGATTGCTCCCACCAGAAGCCTGTGGATTATAGGGCATTTCTGCCGTTGCCGACGGGCGACCATGAAAATAG                                 <  SRR4302540.9212038/94‑1 (MQ=60)

                                                            CAGCTCAGGGTTACTGACCGCCAGATTGCTCCCACCAGAAGCCTGTGGATTATAGGGCATTTCTGCCGTTGCCGACGGGCGACCATGAAAATAG                                 >  SRR4302540.2592058/1‑94 (MQ=60)

                                                            CAGCTCAGGGTTACTGACCGCCAGATTGCTCCCACCAGAAGCCTGTGGATTATAGGGCATTTCTGCCGTTGCCGACGGGCGACCATGAAAATAG                                 <  SRR4302540.5942096/94‑1 (MQ=60)

                                                            CAGCTCAGGGTTACTGACCGCCAGATTGCTCCCACCAGAAGCCTGTGGATTATAGGGCATTTCTGCCGTTGCCGACGGGCGACCATGAAAATAG                                 <  SRR4302540.3444637/94‑1 (MQ=60)

                                                             AGCTCAGGGTTACTGACCGCCAGATTGCTCCCACCAGAAGCCTGTGGATTATAGGGCATTTCTGCCGTTGCCGACGGGCGACCATGAAAATAGC                                >  SRR4302540.5965598/1‑94 (MQ=60)

                                                              agtcaggatacCTGACCGCCACATTGCTCCCACCAGAAGCCTGTGGAGTATAGGGCATTTCTGCCGTTGCCGACGGGCGACCATGAAAATAGCC                               <  SRR4302540.3085071/83‑1 (MQ=60)

                                                              GCTCAGGGTTACTGACCGCCAGATTGCTCCCACCAGAAGCCTGTGGATTATAGGGCATTTCTGCCGTTGCCGACGGGCGACCATGAAAATAGCC                               <  SRR4302540.379157/94‑1 (MQ=60)

                                                               CTCAGGGTTACTGACCGCCAGATTGCTCCCACCAGAAGCCTGTGGATTATAGGGCATTTCTGCCGTTGCCGACGGGCGACCATGAAAATAGCCG                              <  SRR4302540.9735757/94‑1 (MQ=60)

                                                               CTCAGGGTTACTGACCGCCAGATTGCTCCCACCAGAAGCCTGTGGATTATAGGGCATTTCTGCCGTTGCCGACGGGCGACCATGAAAATAGCCG                              >  SRR4302540.3075889/1‑94 (MQ=60)

                                                               CTCAGGGTTACTGACCGCCAGATTGCTCCCACCAGAAGCCTGTGGATTATAGGGCATTTCTGCCGTTGCCGACGGGCGACCATGAAAATAGCCG                              >  SRR4302540.3571019/1‑94 (MQ=60)

                                                               CTCAGGGTTACTGACCGCCAGATTGCTCCCACCAGAAGCCTGTGGATTATAGGGCATTTCTGCCGTTGCCGACGGGCGACCATGAAAATAGCCG                              >  SRR4302540.7344328/1‑94 (MQ=60)

                                                                TCAGGGTTACTGACCGCCAGATTGCTCCCACCAGAAGCCTGTGGATTATAGGGCATTTCTGCCGTTGCCGACGGGCGACCATGAAAATAGCCGT                             <  SRR4302540.7913764/94‑1 (MQ=60)

                                                                TCAGGGTTACTGACCGCCAGATTGCTCCCACCAGAAGCCTGTGGATTATAGGGCATTTCTGCCGTTGCCGACGGGCGACCATGAAAATAGCCGT                             >  SRR4302540.1620149/1‑94 (MQ=60)

                                                                  AGGGTTACTGACCGCCAGATTGCTCCCACCAGAAGCCTGTGGATTATAGGGCATTTCTGCCGTTGCCGACGGGCGACCATGAAAATAGCCGTTG                           <  SRR4302540.4498856/94‑1 (MQ=60)

                                                                  AGGGTTACTGACCGCCAGATTGCTCCCACCAGAAGCCTGTGGATTATAGGGCATTTCTGCCGTTGCCGACGGGCGACCATGAAAATAGCCGTTG                           >  SRR4302540.4102134/1‑94 (MQ=60)

                                                                    GGTTACTGACCGCCAGATTGCTCCCACCAGAAGCCTGTGGATTATAGGGCATTTCTGCCGTTGCCGACGGGCGACCATGAAAATAGCCGTTGCC                         <  SRR4302540.5487917/94‑1 (MQ=60)

                                                                      TTACTGACCGCCAGATTGCTCCCACCAGAAGCCTGTGGATTATAGGGCATTTCTGCCGTTGCCGACGGGCGACCATGAAAATAGCCGTTGCCGG                       >  SRR4302540.1715436/1‑94 (MQ=60)

                                                                       TACTGACCGCCAGATTGCTCCCACCAGAAGCCTGTGGATTATAGGGCATTTCTGCCGTTGCCGACGGGCGACCATGAAAATAGCCGTTGCCGGT                      <  SRR4302540.1577185/94‑1 (MQ=60)

                                                                        ACTGACCGCCAGATTGCTCCCACCAGAAGCCTGTGGATTATAGGGCATTTCTGCCGTTGCCGACGGGCGACCATGAAAATAGACGTTGCCGGTA                     <  SRR4302540.4886708/94‑1 (MQ=60)

                                                                         CTGACCGCCAGATTGCTCCCACCAGAAGCCTGTGGATTATAGGGCATTTCTGCCGTTGCCGACGGGCGACCATGAAAATAGCCGTTGCCGGTAA                    >  SRR4302540.7567457/1‑94 (MQ=60)

                                                                         CTGACCGCCAGATTGCTCCCACCAGAAGCCTGTGGATTATAGGGCATTTCTGCCGTTGCCGACGGGCGACCATGAAAATAGCCGTTGCCGGTAA                    >  SRR4302540.683875/1‑94 (MQ=60)

                                                                              CGCCAGATTGCTCCCACCAGAAGCCTGTGGATTATAGGGCATTTCTGCCGTTGCCGACGGGCGACCATGAAAATAGCCGTTGCCGGTAAAATTC               >  SRR4302540.4984609/1‑94 (MQ=60)

                                                                              CGCCAGATTGCTCCCACCAGAAGCCTGTGGATTATAGGGCATTTCTGCCGTTGCCGACGGGCGACCATGAAAATAGCCGTTGCCGGTAAAATTC               >  SRR4302540.89856/1‑94 (MQ=60)

                                                                              CGCCAGATTGCTCCCACCAGAAGCCTGTGGATTATAGGGCATTTCTGCCGTTGCCGACGGGCGACCATGAAAATAGCCGTTGCCGGTAAAATTC               >  SRR4302540.8934648/1‑94 (MQ=60)

                                                                              CGCCAGATTGCTCCCACCAGAAGCCTGTGGATTATAGGGCATTTCTGCCGTTGCCGACGGGCGACCATGAAAATAGCCGTTGCCGGTAAAATTC               >  SRR4302540.7917991/1‑94 (MQ=60)

                                                                               GCCAGATTGCTCCCACCAGAAGCCTGTGGATTATAGGGCATTTCTGCCGTTGCCGACGGGCGACCATGAAAATAGCCGTTGCCGGTAAAATTCT              >  SRR4302540.1025197/1‑94 (MQ=60)

                                                                               GCCAGATTGCTCCCACCAGAAGCCTGTGGATTATAGGGCATTTCTGCCGTTGCCGACGGGCGACCATGAAAATAGCCGTTGCCGGTAAAATTCT              >  SRR4302540.7780659/1‑94 (MQ=60)

                                                                                CCAGATTGCTCCCACCAGAAGCCTGTGGATTATAGGGCATTTCTGCCGTTGCCGACGGGCGACCATGAAAATAGCCGTTGCCGGTAAAATTCTG             >  SRR4302540.4749087/1‑94 (MQ=60)

                                                                                  AGATTGCTCCCACCAGAAGCCTGTGGATTATAGGGCATTTCTGCCGTTGCCGACGGGCGACCATGAAAATAGCCGTTGCCGGTAAAATTCTGCC           <  SRR4302540.9249464/94‑1 (MQ=60)

                                                                                  AGATTGCTCCCACCAGAAGCCTGTGGATTATAGGGCATTTCTGCCGTTGCCGACGGGCGACCATGAAAATAGCCGTTGCCGGTAAAATTATGCC           >  SRR4302540.1123821/1‑94 (MQ=60)

                                                                                   GATTGCTCCCACCAGAAGCCTGTGGATTATAGGGCATTTCTGCCGTTGCCGACGGGCGACCATGAAAATAGCCGTTGCCGGTAAAATTCTGCCC          >  SRR4302540.1198822/1‑94 (MQ=60)

                                                                                   GATTGCTCCCACCAGAAGCCTGTGGATTATAGGGCATTTCTGCCGTTGCCGACGGGCGACCATGAAAATAGCCGTTGCCGGTAAAATTCTGCCC          >  SRR4302540.522908/1‑94 (MQ=60)

                                                                                    ATTGCTCCCACCAGAAGCCTGTGGATTATAGGGCATTTCTGCCGTTGCCGACGGGCGACCATGAAAATAGCCGTTGCCGGTAAAATTCTGCCCG         <  SRR4302540.2635540/94‑1 (MQ=60)

                                                                                      TGCTCCCACCAGAAGCCTGTGGATTATAGGGCATTTCTGCCGTTGCCGACGGGCGACCATGAAAATAGCCGTTGCCGGTAAAATTCTGCCCGAT       >  SRR4302540.7040416/1‑94 (MQ=60)

                                                                                       GCTCCCACCAGAAGCCTGTGGATTATAGGGCATTTCTGCCGTTGCCGACGGGCGACCATGAAAATAGCCGTTGCCGGTAAAATTCTGCCCGATT      >  SRR4302540.2329236/1‑94 (MQ=60)

                                                                                        CTCCCACCAGAAGCCTGTGGATTATAGGGCATTTCTGCCGTTGCCGACGGGCGACCATGAAAATAGCCGTTGCCGGTAAAATTCTGCCCGATTA     <  SRR4302540.7958431/94‑1 (MQ=60)

                                                                                        CTCCCACCAGAAGCCTGTGGATTATAGGGCATTTCTGCCGTTGCCGACGGGCGACCATGAAAATAGCCGTTGCCGGTAAAATTCTGCCCGATTA     >  SRR4302540.7712923/1‑94 (MQ=60)

                                                                                         TCCCACCAGAAGCCTGTGGATTATAGGGCATTTCTGCCGTTGCCGACGGGCGACCATGAAAATAGCCGTTGCCGGTAAAATTCTGCCCGATTAA    >  SRR4302540.5578669/1‑94 (MQ=60)

                                                                                         TCCCACCAGAAGCCTGTGGATTATAGGGCATTTCTGCCGTTGCCGACGGGCGACCATGAAAATAGCCGTTGCCGGTAAAATTCTGCCCGATTAA    <  SRR4302540.2108279/94‑1 (MQ=60)

                                                                                          CCCACCAGAAGCCTGTGGATTATAGGGCATTTCTGCCGTTGCCGACGGGCGACCATGAAAATAGCCGTTGCCGGTAAAATTCTGCCCGATTAAT   <  SRR4302540.145975/94‑1 (MQ=60)

                                                                                          CCCACCAGAAGCCTGTGGATTATAGGGCATTTCTGCCGTTGCCGACGGGCGACCATGAAAATAGCCGTTGCCGGTAAAATTCTGCCCGATTAAT   >  SRR4302540.410516/1‑94 (MQ=60)

                                                                                           CCACCAGAAGCCTGTGGATTATAGGGCATTTCTGCCGTTGCCGACGGGCGACCATGAAAATAGCCGTTGCCGGTAAAATTCTGCCCGATTAATG  <  SRR4302540.131569/94‑1 (MQ=60)

                                                                                           CCACCAGAAGCCTGTGGATTATAGGGCATTTCTGCCGTTGCCGACGGGCGACCATGAAAATAGCCGTTGCCGGTAAAATTCTGCCCGATTAATG  <  SRR4302540.1058436/94‑1 (MQ=60)

                                                                                           CCACCAGAAGCCTGTGGATTATAGGGCATTTCTGCCGTTGCCGACGGGCGACCATGAAAATAGCCGTTGCCGGTAAAATTCTGCCCGATTAATG  >  SRR4302540.8922889/1‑94 (MQ=60)

CGCGCTGGCATCCGGGTTAGCGGCCCGTAATGCAGCAACGCGTGCGGCTATTAGTTTATCCAGCTCAGGGTTACTGACCGCCAGATTGCTCCCGCCAGAAGCCTGTGGATTATAGGGCATTTCTGCCGTTGCCGACGGGCGACCATGAAAATAGCCGTTGCCGGTAAAATTCTGCCCGATTAATG  >  NC_000913/723861‑724045

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 5 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`