Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 1548 369.5 20095359 96.9% 19472402 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,067,441 (A)6→5 coding (138/174 nt) b4516 → hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,067,4360A.98.8% 1086.8 / 5.2 244coding (133/174 nt)b4516hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (2/1);  new base . (116/124);  total (119/125)
Fisher's exact test for biased strand distribution p-value = 6.13e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 277 total aligned reads displayed.

ACCGCGAAAGAGCATCAGAAGCAGGTAAAAAAGGTGGACAGCACAGCGGGGGTAATTTCAAGAATGACCCGCAGCGCGCATCTGAAGCAGGTAAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAGCCGGGCTAATCAATGACGAATGCATTTTTGTCTGTAGCTCGTCAAAA  >  NC_000913/1067344‑1067524
                                                                                            |                                                                                        
   gcgAAAGAGCATCAGAAGCAGGTAAAAAAGGTGGACAGCACAGCGGGGGTAATTTCAAGAATGACCCGCAGCGCGCATCTGAAGCAGGTaaaa                                                                                       >  1:9757320/1‑93 (MQ=255)
   gcgAAAGAGCATCAGAAGCAGGTAAAAAAGGTGGACAGCACAGCGGGGGTAATTTCAAGAATGACCCGCAGCGCGCATCTGAAGCAGGTaaaa                                                                                       <  2:6607041/93‑1 (MQ=255)
    cgAAAGAGCATCAGAAGCAGGTAAAAAAGGTGGACAGCACAGCGGGGGTAATTTCAAGAATGACCCGCAGCGCGCATCTGAAGCAGGTaaaaa                                                                                      <  2:2204994/93‑1 (MQ=255)
    cgAAAGAGCATCAGAAGCAGGTAAAAAAGGTGGACAGCACAGCGGGGGTAATTTCAAGAATGACCCGCAGCGCGCATCTGAAGCAGGTaaaaa                                                                                      <  2:3946201/93‑1 (MQ=255)
      aaaGAGCATCAGAAGCAGGTAAAAAAGGTGGACAGCACAGCGGGGGTAATTTCAAGAATGACCCGCAGCGCGCATCTGAAGCAGGTaaaaagg                                                                                    >  1:9186932/1‑91 (MQ=255)
      aaaGAGCATCAGAAGCAGGTAAAAAAGGTGGACAGCACAGCGGGGGTAATTTCAAGAATGACCCGCAGCGCGCATCTGAAGCAGGTaaaaagg                                                                                    <  1:2185149/93‑3 (MQ=255)
      aaaGAGCATCAGAAGCAGGTAAAAAAGGTGGACAGCACAGCGGGGGTAATTTCAAGAATGACCCGCAGCGCGCATCTGAAGCAGGTaaaaagg                                                                                    >  2:6512756/1‑91 (MQ=255)
       aaGAGCATCAGAAGCAGGTAAAAAAGGTGGACAGCACAGCGGGGGTAATTTCAAGAATGACCCGCAGCGCGCATCTGAAGCAGGTaaaaaggt                                                                                   >  1:8905001/1‑90 (MQ=255)
       aaGAGCATCAGAAGCAGGTAAAAAAGGTGGACAGCACAGCGGGGGTAATTTCAAGAATGACCCGCAGCGCGCATCTGAAGCAGGTaaaaaggt                                                                                   <  1:9952714/93‑4 (MQ=255)
       aaGAGCATCAGAAGCAGGTAAAAAAGGTGGACAGCACAGCGGGGGTAATTTCAAGAATGACCCGCAGCGCGCATCTGAAGCAGGTaaaaaggt                                                                                   <  1:4357930/93‑4 (MQ=255)
        agagCATCAGAAGCAGGTAAAAAAGGTGGACAGCACAGCGGGGGTAATTTCAAGAATGACCCGCAGCGCGCATCTGAAGCAGGTaaaaaggtg                                                                                  <  1:109306/93‑5 (MQ=255)
        agagCATCAGAAGCAGGTAAAAAAGGTGGACAGCACAGCGGGGGTAATTTCAAGAATGACCCGCAGCGCGCATCTGAAGCAGGTaaaaaggtg                                                                                  >  1:9433577/1‑89 (MQ=255)
           gCATCAGAAGCAGGTAAAAAAGGTGGACAGCACAGCGGGGGTAATTTCAAGAATGACCCGCAGCGCGCATCTGAAGCAGGT‑AAAAAGGTGGTa                                                                              <  2:7373684/93‑1 (MQ=255)
             aTCAGAAGCAGGTAAAAAAGGTGGACAGCACAGCGGGGGTAATTTCAAGAATGACCCGCAGCGCGCATCTGAAGCAGGT‑AAAAAGGTGGTAag                                                                            <  2:8362692/93‑1 (MQ=255)
              tCAGAAGCAGGTAAAAAAGGTGGACAGCACAGCGGGGGTAATTTCAAGAATGACCCGCAGCGCGCATCTGAAGCAGGT‑AAAAAGGTGGTAaga                                                                           >  1:2874481/1‑93 (MQ=255)
              tCAGAAGCAGGTAAAAAAGGTGGACAGCACAGCGGGGGTAATTTCAAGAATGACCCGCAGCGCGCATCTGAAGCAGGT‑AAAAAGGTGGTAaga                                                                           >  1:2321346/1‑93 (MQ=255)
                aGAAGCAGGTAAAAAAGGTGGACAGCACAGCGGGGGTAATTTCAAGAATGACCCGCAGCGCGCATCTGAAGCAGGT‑AAAAAGGTGGTAAGAGc                                                                         >  1:5837224/1‑93 (MQ=255)
                 gAAGCAGGTAAAAAAGGTGGACAGCACAGCGGGGGTAATTTCAAGAATGACCCGCAGCGCGCATCTGAAGCAGGT‑AAAAAGGTGGTAAGAGCa                                                                        <  1:444133/93‑1 (MQ=255)
                  aaGCAGGTAAAAAAGGTGGACAGCACAGCGGGGTTAATTTCAAGAATGACCCGCAGCGCGCATCTGAAGCAGGT‑AAAAAGGTGGTAAGAGCAg                                                                       <  2:5429439/93‑1 (MQ=255)
                     cAGGTAAAAAAGGTGGACAGCACAGCGGGGGTAATTTCAAGAATGACCCGCAGCGCGCATCTGAAGCAGGT‑AAAAAGGTGGTAAGAGCAGTCa                                                                    <  2:8778695/93‑1 (MQ=255)
                       ggTAAAAAAGGTGGACAGCACAGCGGGGGTAATTTCAAGAATGACCCGCAGCGCGCATCTGAAGCAGGT‑AAAAAGGTGGTAAGAGCAGTCACg                                                                  <  2:4542128/93‑1 (MQ=255)
                        gTAAAAAAGGTGGACAGCACAGCGGGGGTAATTTCAAGAATGACCCGCAGCGCGCATCTGAAGCAGGT‑AAAAAGGTGGTAAGAGCAGTCACgg                                                                 >  2:240345/1‑93 (MQ=255)
                        gTAAAAAAGGTGGACAGCACAGCGGGGGTAATTTCAAGAATGACCCGCAGCGCGCATCTGAAGCAGGT‑AAAAAGGTGGTAAGAGCAGTCACgg                                                                 <  2:5516128/93‑1 (MQ=255)
                         tAAAAAAGGTGGACAGCACAGCGGGGGTAATTTCAAGAATGACCCGCAGCGCGCATCTGAAGCAGGT‑AAAAAGGTGGTAAGAGCAGTCACGGc                                                                <  2:1382704/93‑1 (MQ=255)
                          aaaaaaGGTGGACAGCACAGCGGGGGTAATTTCAAGAATGACCCGCAGCGCGCATCTGAAGCAGGT‑AAAAAGGTGGTAAGAGCAGTCACGGCa                                                               <  2:9876951/93‑1 (MQ=255)
                           aaaaaGGTGGACAGCACAGCGGGGGTAATTTCAAGAATGACCCGCAGCGCGCATCTGAAGCAGGT‑AAAAAGGTGGTAAGAGCAGTCACGGCaa                                                              <  1:9464903/93‑1 (MQ=255)
                           aaaaaGGTGGACAGCACAGCGGGGGTAATTTCAAGAATGACCCGCAGCGCGCATCTGAAGCAGGT‑AAAAAGGTGGTAAGAGCAGTCACGGCaa                                                              >  1:1337610/1‑93 (MQ=255)
                           aaaaaGGTGGACAGAACAGCGGGGGTAATTTCAAGAATGACCCGCAGCGCGCATCTGAAGCAGGT‑AAAAAGGTGGTAAGAGCAGTCACGGCaa                                                              >  1:6847181/1‑93 (MQ=255)
                             aaaGGTGGACAGCACAGCGGGGGTAATTTCAAGAATGACCCGCAGCGCGCATCTGAAGCAGGT‑AAAAAGGTGGTAAGAGCAGTCACGGCaaaa                                                            <  1:7045926/93‑1 (MQ=255)
                             aaaGGTGGACAGCACAGCGGGGGTAATTTCAAGAATGACCCGCAGCGCGCATCTGAAGCAGGT‑AAAAAGGTGGTAAGAGCAGTCACGGCaaaa                                                            <  1:7369277/93‑1 (MQ=255)
                              aaGGTGGACAGCACAGCGGGGGTAATTTCAAGAATGACCCGCAGCGCGCATCTGAAGCAGGT‑AAAAAGGTGGTAAGAGCAGTCACGGCAAAAg                                                           >  2:47182/1‑93 (MQ=255)
                                 gTGGACAGCACAGCGGGGGTAATTTCAAGAATGACCCGCAGCGCGCATCTGAAGCAGGT‑AAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGa                                                        <  1:8409068/93‑1 (MQ=255)
                                   ggACAGCGGAGCGGGGGTAATTTCAAGAATGACCAGCAGCGCGCATCTGAAGCA‑GCAAAAAAGGTGGTAAGATCAGTCACGGCAAAAGCGACa                                                      <  1:5567983/93‑1 (MQ=255)
                                   ggACAGCACAGCGGGGGTAATTTCAAGAATGACCCGCAGCGCGCATCTGAAGCAGGT‑AAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACa                                                      >  2:8194605/1‑93 (MQ=255)
                                   ggACAGCACAGCGGGGGTAATTTCAAGAATGACCCGCAGCGCGCATCTGAAGCAGGT‑AAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACa                                                      >  2:5387756/1‑93 (MQ=255)
                                    gACAGCACAGCGGGGGTAATTTCAAGAATGACCCGCAGCGCGCATCTGAAGCAGGT‑AAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACaa                                                     <  2:1598100/93‑1 (MQ=255)
                                    gACAGCACAGCGGGGGTAATTTCAAGAATGACCCGCAGCGCGCATCTGAAGCAGGT‑AAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACaa                                                     <  2:9173013/93‑1 (MQ=255)
                                    gACAGCACAGCGGGGGTAATTTCAAGAATGACCCGCAGCGCGCATCTGAAGCAGGT‑AAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACaa                                                     <  2:7823681/93‑1 (MQ=255)
                                     acagcacagcGGGGGTAATTTCAAGAATGACCCGCAGCGCGCATCTGAAGCAGGT‑AAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAAc                                                    >  2:3011216/1‑93 (MQ=255)
                                      cagcacagcGGGGGTAATTACAAGAATGACCCGCAGCGCGCATCTGAAGCAGGT‑AAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACt                                                   >  2:5753191/1‑93 (MQ=255)
                                       agcacagcGGGGGTAATTTCAAGAATGACCCGCAGCGCGCATCTGAAGCAGGT‑AAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTa                                                  >  2:5262651/1‑93 (MQ=255)
                                       agcacagcGGGGGTAATTTCAAGAATGACCCGCAGCGCGCATCTGAAGCAGGT‑AAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTa                                                  <  2:3218189/93‑1 (MQ=255)
                                        gcacagcGGGGGTAATTTCAAGAATGACCCGCAGCGCGCATCTGAAGCAGGT‑AAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAg                                                 <  2:1527310/93‑1 (MQ=255)
                                         cacagcGGGGGTAATTTCAAGAATGACCCGCAGCGCGCATCTGAAGCAGGT‑AAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAGc                                                <  1:3191910/93‑1 (MQ=255)
                                          acagcGGGGGTAATTTCAAGAATGACCCGCAGCGCGCATCTGAAGCAGGT‑AAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAgtc                                               >  1:6953250/1‑91 (MQ=255)
                                          acagcGGGGGTAATTTCAAGAATGACCCGCAGCGCGCATCTGAAGCAGGT‑AAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAGcc                                               <  2:369973/93‑1 (MQ=255)
                                          acagcGGGGGTAATTTCAAGAATGACCCGCAGCGCGCATCTGAAGCAGGT‑AAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAGcc                                               >  1:7345375/1‑93 (MQ=255)
                                           cagcGGGGGTAATTTCAAGAATGACCCGCAGCGCGCATCTGAAGCAGGTAAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAGcc                                               >  2:3602524/1‑93 (MQ=255)
                                              ggggggTAATTTCAAGAATGACCCGCAGCGCGCATCTGAAGCAGGT‑AAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAGCCGGGc                                           <  2:5065924/92‑1 (MQ=255)
                                              cGGGGGTAATTTCAAGAATGACCCGCAGCGCGCATCTGAAGCAGGT‑AAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAGCCGGGc                                           <  2:4450477/93‑1 (MQ=255)
                                                ggggTAATTTCAAGAATGACCCGCAGCGCGCATCTGAAGCAGGT‑AAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAGCCGGGCTa                                         <  2:787764/93‑1 (MQ=255)
                                                 gggTAATTTCAAGAATGACCCGCAGCGCGCATCTGAAGCAGGT‑AAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAGCCGGGCTaa                                        <  1:8658014/93‑1 (MQ=255)
                                                  ggTAATTTCAAGAATGACCCGCAGCGCGCATCTGAAGCAGGT‑AAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAGCCGGGCTAAt                                       <  2:8824828/93‑1 (MQ=255)
                                                  ggTAATTTCAAGAATGACCCGCAGCGCGCATCTGAAGCAGGT‑AAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAGCCGGGCTAAt                                       <  2:768013/93‑1 (MQ=255)
                                                  ggTAATTTCAAGAATGACCCGCAGCGCGCATCTGAAGCAGGT‑AAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAGCCGGGCTAAt                                       <  1:4009358/93‑1 (MQ=255)
                                                     aaTTTCAAGAATGACCCGCAGCGCGCATCTGAAGCAGGT‑AAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAGCCGGGCTAATCaa                                    <  1:8031204/93‑1 (MQ=255)
                                                     aaTTTCAAGAATGACCCGCAGCGCGCATCTGAAGCAGGT‑AAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAGCCGGGCTAATCaa                                    <  2:6779940/93‑1 (MQ=255)
                                                      aTTTCAAGAATGACCCGCAGCGCGCATCTGAAGCAGGT‑AAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAGCCGGGCTAATCAAt                                   <  2:8546139/93‑1 (MQ=255)
                                                      aTTTCAAGAATGACCCGCAGCGCGCATCTGAAGCAGGT‑AAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAGCCGGGCTAATCAAt                                   <  1:606473/93‑1 (MQ=255)
                                                      aTTTCAAGAATGACCCGCAGCGCGCATCTGAAGCAGGT‑AAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAGCCGGGCTAATCAAt                                   >  1:3770171/1‑93 (MQ=255)
                                                       tttCAAGAATGACCCGCAGCGCGCATCTGAAGCAGGT‑AAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAGCCGGGCTAATCAATg                                  >  2:2571292/1‑93 (MQ=255)
                                                       tttCAAGAATGACCCGCAGCGCGCATCTGAAGCAGGT‑AAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAGCCGGGCTAATCAATg                                  >  1:3545984/1‑93 (MQ=255)
                                                       tttCAAGAATGACCCGCAGCGCGCATCTGAAGCAGGT‑AAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAGCCGGGCTAATCAATg                                  >  2:5881251/1‑93 (MQ=255)
                                                         tCAAGAATGACCCGCAGCGCGCATCTGAAGCAGGT‑AAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAGCCGGGCTAATCAATGAc                                <  1:4085859/93‑1 (MQ=255)
                                                         tCAAGAATGACCCGCAGCGCGCATCTGAAGCAGGT‑AAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAGCCGGGCTAATCAATGAc                                <  1:188533/93‑1 (MQ=255)
                                                            aGAATGACCCGCAGCGCGCATCTGAAGCAGGT‑AAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAGCCGGGCTAATCAATGACGaa                             <  1:5472760/93‑1 (MQ=255)
                                                             gAATGACCCGCAGCGCGCATCTGAAGCAGGT‑AAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAGCCGGGCTAATCAATGACGAAt                            <  1:7868332/93‑1 (MQ=255)
                                                                tGACCCGCAGCGCGCATCTGAAGCAGGT‑AAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAGCCGGGCTAATCAATGACGAATGCa                         >  1:8107298/1‑93 (MQ=255)
                                                                  aCCCGCAGCGCGCATCTGAAGCAGGT‑AAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAGCCGGGCTAATCAATGACGAATGCAtt                       >  2:3866089/1‑93 (MQ=255)
                                                                   cccGCAGCGCGCATCTGAAGCAGGT‑AAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAGCCGGGCTAATCAATGACGAATGCAttt                      >  2:4129659/1‑93 (MQ=255)
                                                                   cccGCAGCGCGCATCTGAAGCAGGT‑AAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAGCCGGGCTAATCAATGACGAATGCAttt                      <  1:1701998/93‑1 (MQ=255)
                                                                    ccGCAGCGCGCATCTGAAGCAGGT‑AAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAGCCGGGCTAATCAATGACGAATGCAtttt                     >  2:6066682/1‑93 (MQ=255)
                                                                     cGCAGCGCGCATCTGAAGCAGGT‑AAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAGCCGGGCTAATCAATGACGAATGCAttttt                    >  2:5700832/1‑93 (MQ=255)
                                                                      gCAGCGCGCATCTGAAGCAGGT‑AAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAGCCGGGCTAATCAATGACGAATGCATTTTTg                   <  2:7115128/93‑1 (MQ=255)
                                                                      gCAGCGCGCATCTGAAGCAGGT‑AAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAGCCGGGCTAATCAATGACGAATGCATTTTTg                   >  2:3452570/1‑93 (MQ=255)
                                                                        aGCGCGCATCTGAAGCAGGT‑AAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAGCCGGGCTAATCAATGACGAATGCATTTTTGTc                 <  2:9358167/93‑1 (MQ=255)
                                                                        aGCGCGCATCTGAAGCAGGT‑AAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAGCCGGGCTAATCAATGACGAATGCATTTTTGTc                 >  1:3052713/1‑93 (MQ=255)
                                                                        aGCGCGCATCTGAAGCAGGT‑AAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAGCCGGGCTAATCAATGACGAATGCATTTTTGTc                 <  2:8607570/93‑1 (MQ=255)
                                                                        aGCGCGCATCTGAAGCAGGT‑AAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAGCCGGGCTAATCAATGACGAATGCATTTTTGTc                 <  1:627319/93‑1 (MQ=255)
                                                                         gcgcgcATCTGAAGCAGGT‑AAAACGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAGCCGGGCTAATCAATGACGAATGCATTTTTGTCt                <  2:5604677/93‑1 (MQ=255)
                                                                         gcgcgcATCTGAAGCAGGT‑AAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAGCCGGGCTAATCAATGACGAATGCATTTTTGTCt                <  2:2941009/93‑1 (MQ=255)
                                                                         gcgcgcATCTGAAGCAGGT‑AAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAGCCGGGCTAATCAATGACGAATGCATTTTTGTCt                >  1:5351304/1‑93 (MQ=255)
                                                                          cgcgcATCTGAAGCAGGT‑AAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAGCCGGGCTAATCAATGACGAATGCATTTTTGTCTg               >  2:3074691/1‑93 (MQ=255)
                                                                           gcgcATCTGAAGCAGGT‑AAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAGCCGGGCTAATCAATGACGAATGCATTTTTGTCTGt              <  1:1021183/93‑1 (MQ=255)
                                                                            cgcATCTGAAGCAGGT‑AAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAGCCGGGCTAATCAATGACGAATGCATTTTTGTCTGTa             >  2:2803697/1‑93 (MQ=255)
                                                                              cATCTGAAGCAGGT‑AAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAGCCGGGCTAATCAATGACGAATGCATTTTTGTCTGTAGc           <  2:1168219/93‑1 (MQ=255)
                                                                              cATCTGAAGCAGGT‑AAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAGCCGGGCTAATCAATGACGAATGCATTTTTGTCTGTAGc           <  1:5128575/93‑1 (MQ=255)
                                                                               aTCTGAAGCAGGT‑AAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAGCCGGGCTAATCAATGACGAATGCATTTTTGTCTGTAGCt          >  1:7741771/1‑93 (MQ=255)
                                                                               aTCTGAAGCAGGT‑AAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAGCCGGGCTAATCAATGACGAATGCATTTTTGTCTGTAGCt          >  1:4678812/1‑93 (MQ=255)
                                                                               aTCTGAAGCAGGT‑AAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAGCCGGGCTAATCAATGACGAATGCATTTTTGTCTGTAGCt          <  1:6860168/93‑1 (MQ=255)
                                                                                tCTGAAGCAGGT‑AAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAGCCGGGCTAATCAATGACGAATGCATTTTTGTCTGTAGCTc         >  1:566997/1‑93 (MQ=255)
                                                                                 cTGAAGCAGGT‑AAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAGCCGGGCTAATCAATGACGAATGCATTTTTGTCTGTAGCTCg        <  2:4635011/93‑1 (MQ=255)
                                                                                  tGAAGCAGGT‑AAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAGCCGGGCTAATCAATGACGAATGCATTTTTGTCTGTAGCTCGt       >  1:9287155/1‑93 (MQ=255)
                                                                                  tGAAGCAGGT‑AAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAGCCGGGCTAATCAATGACGAATGCATTTTTGTCTGTAGCTCGt       <  2:1876239/93‑1 (MQ=255)
                                                                                   gAAGCAGGT‑AAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAGCCGGGCTAATCAATGACGAATGCATTTTTGTCTGTAGCTCGta      >  1:2504870/1‑92 (MQ=255)
                                                                                    aaGCAGGT‑AAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAGCCGGGCTAATCAATGACGAATGCATTTTTGTCTGTAGCTCGTCa     >  2:1999317/1‑93 (MQ=255)
                                                                                     aGCAGGT‑AAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAGCCGGGCTAATCAATGACGAATGCATTTTTGTCTGTAGCTCGTCaa    >  2:6973009/1‑93 (MQ=255)
                                                                                     aGCAGGT‑AAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAGCCGGGCTAATCAATGACGAATGCATTTTTGTCTGTAGCTCGTCaa    >  2:4980076/1‑93 (MQ=255)
                                                                                     aGCAGGT‑AAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAGCCGGGCTAATCAATGACGAATGCATTTTTGTCTGTAGCTCGTCaa    >  2:4056446/1‑93 (MQ=255)
                                                                                      gCAGGT‑AAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAGCCGGGCTAATCAATGACGAATGCATTTTTGTCTGTAGCTCGTCaaa   >  2:1272503/1‑93 (MQ=255)
                                                                                            |                                                                                        
ACCGCGAAAGAGCATCAGAAGCAGGTAAAAAAGGTGGACAGCACAGCGGGGGTAATTTCAAGAATGACCCGCAGCGCGCATCTGAAGCAGGTAAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAGCCGGGCTAATCAATGACGAATGCATTTTTGTCTGTAGCTCGTCAAAA  >  NC_000913/1067344‑1067524

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GAAAGAGCATCAGAAGCAGGTAAAAAAGGTGGACAGCACAGCGGGGGTAATTTCAAGAATGACCCGCAGCGCGCATCTGAAGCAGGTAAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAGCCGGGCTAATCAATGACGAATGCATTTTTGTCTGTAGCTCGTCAAAAAGCCATCACC  >  NC_000913/1067349‑1067534
                                                                                            |                                                                                             
GAAAGAGCATCAGAAGCAGGTAAAAAAGGTGGACAGCACAGCGGGGGTAATTTCAAGAATGACCCGCAGCGCGCATCTGAAGCAGGTAAAAAGG                                                                                              >  SRR4302540.9297106/1‑94 (MQ=60)
GAAAGAGCATCAGAAGCAGGTAAAAAAGGTGGACAGCACAGCGGGGGTAATTTCAAGAATGACCCGCAGCGCGCATCTGAAGCAGGTAAAAAGG                                                                                              >  SRR4302540.5290731/1‑94 (MQ=60)
 AAAGAGCATCAGAAGCAGGTAAAAAAGGTGGACAGCACAGCGGGGGTAATTTCAAGAATGACCCGCAGCGCGCATCTGAAGCAGGT‑AAAAAGGT                                                                                            >  SRR4302540.9008106/1‑94 (MQ=60)
 AAAGAGCATCAGAAGCAGGTAAAAAAGGTGGACAGCACAGCGGGGGTAATTTCAAGAATGACCCGCAGCGCGCATCTGAAGCAGGT‑AAAAAGGT                                                                                            >  SRR4302540.620034/1‑94 (MQ=60)
 AAAGAGCATCAGAAGCAGGTAAAAAAGGTGGACAGCACAGCGGGGGTAATTTCAAGAATGACCCGCAGCGCGCATCTGAAGCAGGT‑AAAAAGGT                                                                                            <  SRR4302540.2208738/94‑1 (MQ=60)
  AAGAGCATCAGAAGCAGGTAAAAAAGGTGGACAGCACAGCGGGGGTAATTTCAAGAATGACCCGCAGCGCGCATCTGAAGCAGGT‑AAAAAGGTG                                                                                           <  SRR4302540.10068896/94‑1 (MQ=60)
  AAGAGCATCAGAAGCAGGTAAAAAAGGTGGACAGCACAGCGGGGGTAATTTCAAGAATGACCCGCAGCGCGCATCTGAAGCAGGT‑AAAAAGGTG                                                                                           >  SRR4302540.9545623/1‑94 (MQ=60)
  AAGAGCATCAGAAGCAGGTAAAAAAGGTGGACAGCACAGCGGGGGTAATTTCAAGAATGACCCGCAGCGCGCATCTGAAGCAGGT‑AAAAAGGTG                                                                                           <  SRR4302540.5519023/94‑1 (MQ=60)
  AAGAGCATCAGAAGCAGGTAAAAAAGGTGGACAGCACAGCGGGGGTAATTTCAAGAATGACCCGCAGCGCGCATCTGAAGCAGGT‑AAAAAGGTG                                                                                           <  SRR4302540.4405325/94‑1 (MQ=60)
  AAGAGCATCAGAAGCAGGTAAAAAAGGTGGACAGCACAGCGGGGGTAATTTCAAGAATGACCCGCAGCGCGCATCTGAAGCAGGT‑AAAAAGGTG                                                                                           >  SRR4302540.1659262/1‑94 (MQ=60)
   AGAGCATCAGAAGCAGGTAAAAAAGGTGGACAGCACAGCGGGGGTAATTTCAAGAATGACCCGCAGCGCGCATCTGAAGCAGGT‑AAAAAGGTGG                                                                                          <  SRR4302540.5643552/94‑1 (MQ=60)
   AGAGCATCAGAAGCAGGTAAAAAAGGTGGACAGCACAGCGGGGGTAATTTCAAGAATGACCCGCAGCGCGCATCTGAAGCAGGT‑AAAAAGGTGG                                                                                          <  SRR4302540.110477/94‑1 (MQ=60)
       CATCAGAAGCAGGTAAAAAAGGTGGACAGCACAGCGGGGGTAATTTCAAGAATGACCCGCAGCGCGCATCTGAAGCAGGT‑AAAAAGGTGGTAAG                                                                                      <  SRR4302540.481167/94‑1 (MQ=60)
        ATCAGAAGCAGGTAAAAAAGGTGGACAGCACAGCGGGGGTAATTTCAAGAATGACCCGCAGCGCGCATCTGAAGCAGGT‑AAAAAGGTGGTAAGA                                                                                     >  SRR4302540.2346346/1‑94 (MQ=60)
        ATCAGAAGCAGGTAAAAAAGGTGGACAGCACAGCGGGGGTAATTTCAAGAATGACCCGCAGCGCGCATCTGAAGCAGGT‑AAAAAGGTGGTAAGA                                                                                     >  SRR4302540.4208189/1‑94 (MQ=60)
        ATCAGAAGCAGGTAAAAAAGGTGGACAGCACAGCGGGGGTAATTTCAAGAATGACCCGCAGCGCGCATCTGAAGCAGGT‑AAAAAGGTGGTAAGA                                                                                     >  SRR4302540.2905732/1‑94 (MQ=60)
         TCAGAAGCAGGTAAAAAAGGTGGACAGCACAGCGGGGGTAATTTCAAGAATGACCCGCAGCGCGCATCTGAAGCAGGT‑AAAAAGGTGGTAAGAG                                                                                    <  SRR4302540.2491295/94‑1 (MQ=60)
          CAGAAGCAGGTAAAAAAGGTGGACAGCACAGCGGGGGTAATTTCAAGAATGACCCGCAGCGCGCATCTGAAGCAGGT‑AAAAAGGTGGTAAGAGC                                                                                   >  SRR4302540.5901766/1‑94 (MQ=60)
          CAGAAGCAGGTAAAAAAGGTGGACAGCACAGCGGGGGTAATTTCAAGAATGACCCGCAGCGCGCATCTGAAGCAGGT‑AAAAAGGTGGTAAGAGC                                                                                   >  SRR4302540.2621574/1‑94 (MQ=60)
            GAAGCGGGTAACAAATGTGGACAGCACAGCGTGGGTAATTTCAAGAATGACCCGCAGCGCGCATCTGAAGCAGGT‑AAAAAGGTGGTAAGAGCAG                                                                                 <  SRR4302540.448864/94‑1 (MQ=60)
            GAAGCAGGTAAAAAAGGTGGACAGCACAGCGGGGGTAATTTCAAGAATGACCCGCAGCGCGCATCTGAAGCAGGT‑AAAAAGGTGGTAAGAGCAG                                                                                 <  SRR4302540.3661078/94‑1 (MQ=60)
            GAAGCAGGTAAAAAAGGTGGACAGCACAGCGGGGGTAATTTCAAGAATGACCCGCAGCGCGCATCTGAAGCAGGT‑AAAAAGGTGGTAAGAGCAG                                                                                 <  SRR4302540.448863/94‑1 (MQ=60)
              AGCAGGTAAAAAAGGTGGACAGCACAGCGGGGGTAATTTCAAGAATGACCCGCAGCGCGCATCTGAAGCAGGT‑AAAAAGGTGGTAAGAGCAGTC                                                                               >  SRR4302540.7976421/1‑94 (MQ=60)
              AGCAGGTAAAAAAGGTGGACAGCACAGCGGGGGTAATTTCAAGAATGACCCGCAGCGCGCATCTGAAGCAGGT‑AAAAAGGTGGTAAGAGCAGTC                                                                               <  SRR4302540.3444429/94‑1 (MQ=60)
                CAGGTAAAAAAGGTGGACAGCACAGCGGGGGTAATTTCAAGAATGACCCGCAGCGCGCATCTGAAGCAGGT‑AAAAAGGTGGTAAGAGCAGTCAC                                                                             >  SRR4302540.7279447/1‑94 (MQ=60)
                   GTAAAAAAGGTGGACAGCACAGCGGGGGTAATTTCAAGAATGACCCGCAGCGCGCATCTGAAGCAGGT‑AAAAAGGTGGTAAGAGCAGTCACGGC                                                                          <  SRR4302540.7540995/94‑1 (MQ=60)
                     AAAAAAGGTGGACAGCACAGCGGGGGTAATTTCAAGAATGACCCGCAGCGCGCATCTGAAGCAGGT‑AAAAAGGTGGTAAGAGCAGTCACGGCAA                                                                        >  SRR4302540.9276950/1‑94 (MQ=60)
                     AAAAAAGGTGGACAGCACAGCGGGGGTAATTTCAAGAATGACCCGCAGCGCGCATCTGAAGCAGGT‑AAAAAGGTGGTAAGAGCAGTCACGGCAA                                                                        >  SRR4302540.1351612/1‑94 (MQ=60)
                     AAAAAAGGTGGACAGCACAGCGGGGGTAATTTCAAGAATGACCCGCAGCGCGCATCTGAAGCAGGT‑AAAAAGGTGGTAAGAGCAGTCACGGCAA                                                                        >  SRR4302540.7439500/1‑94 (MQ=60)
                     AAAAAAGGTGGACAGAACAGCGGGGGTAATTTCAAGAATGACCCGCAGCGCGCATCTGAAGCAGGT‑AAAAAGGTGGTAAGAGCAGTCACGGCAA                                                                        >  SRR4302540.6923037/1‑94 (MQ=60)
                      AAAAAGGTGGACAGCACAGCGGGGGTAATTTCAAGAATGACCCGCAGCGCGCATCTGAAGCAGGT‑AAAAAGGTGGTAAGAGCAGTCACGGCAAA                                                                       <  SRR4302540.9577189/94‑1 (MQ=60)
                      AAAAAGGTGGACAGCACAGCGGGGGTAATTTCAAGAATGACCCGCAGCGCGCATCTGAAGCAGGT‑AAAAAGGTGGTAAGAGCAGTCACGGCAAA                                                                       >  SRR4302540.7047101/1‑94 (MQ=60)
                        AAAGGTGGACAGCACAGCGGGGGTAATTTCAAGAATGACCCGCAGCGCGCATCTGAAGCAGGT‑AAAAAGGTGGTAAGAGCAGTCACGGCAAAAG                                                                     <  SRR4302540.7124276/94‑1 (MQ=60)
                        AAAGGTGGACAGCACAGCGGGGGTAATTTCAAGAATGACCCGCAGCGCGCATCTGAAGCAGGT‑AAAAAGGTGGTAAGAGCAGTCACGGCAAAAG                                                                     <  SRR4302540.7858384/94‑1 (MQ=60)
                        AAAGGTGGACAGCACAGCGGGGGTAATTTCAAGAATGACCCGCAGCGCGCATCTGAAGCAGGT‑AAAAAGGTGGTAAGAGCAGTCACGGCAAAAG                                                                     <  SRR4302540.7451423/94‑1 (MQ=60)
                         AAGGTGGACAGCACAGCGGGGGTAATTTCAAGAATGACCCGCAGCGCGCATCTGAAGCAGGT‑AAAAAGGTGGTAAGAGCAGTCACGGCAAAAGC                                                                    >  SRR4302540.8461949/1‑94 (MQ=60)
                            GTGGACAGCACAGCGGGGGTAATTTCAAGAATGACCCGCAGCGCGCATCTGAAGCAGGT‑AAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGAC                                                                 <  SRR4302540.8506669/94‑1 (MQ=60)
                            GTGGACAGCACAGCGGGGGTAATTTCAAGAATGACCCGCAGCGCGCATCTGAAGCAGGT‑AAAAAGGTGGTAAGAGCAGTCACGGCAAAAGAGAC                                                                 >  SRR4302540.8973118/1‑94 (MQ=60)
                             ggggCAGCACAGCGGGGGTAATTTCAAGAATGACCCGCAGCGCGCATCTGAAGCAGGT‑AAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACA                                                                <  SRR4302540.8879957/90‑1 (MQ=60)
                             TGGACAGCACAGCGGGGGTAATTTCAAGAATGACCCGCAGCGCGCATCTGAAGCAGGT‑AAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACA                                                                >  SRR4302540.3686277/1‑94 (MQ=60)
                             TGGACAGCACAGCGGGGGTAATTTCAAGAATGACCCGCAGCGCGCATCTGAAGCAGGT‑AAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACA                                                                <  SRR4302540.2171741/94‑1 (MQ=60)
                             GGGACAGCACAGCGGGGGTAATTTCAAGAATGACCCGCAGCGCGCATCTGAAGCAGGT‑AAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACA                                                                <  SRR4302540.3129339/94‑1 (MQ=60)
                             GGGACAGCACAGCGGGGGTAATTGCAAGAATGACCCGCAGCGCGCATCTGAAGCAGGT‑AAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACA                                                                <  SRR4302540.3129340/94‑1 (MQ=60)
                              GGACAGCGGAGCGGGGGTAATTTCAAGAATGACCAGCAGCGCGCATCTGAAGCA‑GCAAAAAAGGTGGTAAGATCAGTCACGGCAAAAGCGACAA                                                               <  SRR4302540.5629295/94‑1 (MQ=60)
                                GCAGCACAGCGGGGGTAATTTCAAGAATGACCCGCAGCGCGCATCTGAAGCAGGT‑AAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACT                                                             <  SRR4302540.4482026/94‑1 (MQ=60)
                                ACAGCACAGCGGGGGTAATTTCAAGAATGACCCGCAGCGCGCATCTGAAGCAGGT‑AAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACT                                                             <  SRR4302540.2327263/94‑1 (MQ=60)
                                ACAGCACAGCGGGGGTAATTTCAAGAATGACCCGCAGCGCGCATCTGAAGCAGGT‑AAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACT                                                             <  SRR4302540.5612047/94‑1 (MQ=60)
                                 CAGCACAGCGGGGGTAATTTCAAGAATGACCCGCAGCGCGCATCTGAAGCAGGT‑AAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTA                                                            <  SRR4302540.8628847/94‑1 (MQ=60)
                                 CAGCACAGCGGGGGTAATTTCAAGAATGACCCGCAGCGCGCATCTGAAGCAGGT‑AAAAAGGCGGTAAGAGCAGTCACGGCAAAAGCGACAACTA                                                            >  SRR4302540.4627618/1‑94 (MQ=60)
                                    CACAGCGGGGGTAATTTCAAGAATGACCCGCAGCGCGCATCTGAAGCAGGT‑AAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAGTC                                                         >  SRR4302540.7030609/1‑94 (MQ=60)
                                    CACAGCGGGGGTAATTTCAAGAATGACCCGCAGCGCGCATCTGAAGCAGGT‑AAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAGCC                                                         <  SRR4302540.3226580/94‑1 (MQ=60)
                                    CACAGCGGGGGTAATTTCAAGAATGACCCGCAGCGCGCATCTGAAGCAGGT‑AAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAGCC                                                         >  SRR4302540.7427330/1‑94 (MQ=60)
                                       AGCGGGGGTAATTTCAAGAATGACCCGCAGCGCGCATCTGAAGCAGGT‑AAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAGCCGGG                                                      <  SRR4302540.6162476/94‑1 (MQ=60)
                                       AGCGGGGGTAATTTCAAGAATGACCCGCAGCGCGCATCTGAAGCAGGT‑AAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAGCCGGG                                                      <  SRR4302540.2414866/94‑1 (MQ=60)
                                        GCGGGGGTAATTTCAAGAATGACCCGCAGCGCGCATCTGAAGCAGGT‑AAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAGCCGGGC                                                     <  SRR4302540.8753477/94‑1 (MQ=60)
                                          GGGGGTAATTTCAAGAATGACCCGCAGCGCGCATCTGAAGCAGGT‑AAACAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAGCCGGGCTA                                                   >  SRR4302540.3998477/1‑94 (MQ=60)
                                          GGGGGTAATTTCAAGAATGACCCGCAGCGCGCATCTGAAGCAGGT‑AAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAGCCGGGCTA                                                   >  SRR4302540.7922270/1‑94 (MQ=60)
                                          GGGGGTAATTTCAAGAATGACCCGCAGCGCGCATCTGAAGCAGGT‑AAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAGCCGGGCTA                                                   <  SRR4302540.10021885/94‑1 (MQ=60)
                                           GGGGTAATTTCAAGAATGACCCGCAGCGCGCATCTGAAGCAGGT‑AAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAGCCGGGCTAA                                                  >  SRR4302540.95976/1‑94 (MQ=60)
                                           GGGGTAATTTCAAGAATGACCCGCAGCGCGCATCTGAAGCAGGT‑AAAAAGGTGGTAAGAGCAGTCACGGCAAAAGAGACAACTAGCCGGGCTAA                                                  >  SRR4302540.95973/1‑94 (MQ=60)
                                            GGGTAATTTCAAGAATGACCCGCAGCGCGCATCTGAAGCAGGT‑AAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAGCCGGGCTAAT                                                 <  SRR4302540.8758585/94‑1 (MQ=60)
                                            GGGTAATTTCAAGAATGACCCGCAGCGCGCATCTGAAGCAGGT‑AAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAGCCGGGCTAAT                                                 <  SRR4302540.7052168/94‑1 (MQ=60)
                                             GGTAATTTCAAGAATGACCCGCAGCGCGCATCTGAAGCAGGT‑AAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAGCCGGGCTAATC                                                <  SRR4302540.4052532/94‑1 (MQ=60)
                                             GGTAATTTCAAGAATGACCCGCAGCGCGCATCTGAAGCAGGT‑AAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAGCCGGGCTAATC                                                <  SRR4302540.1080075/94‑1 (MQ=60)
                                              GTAATTTCAAGAATGACCCGCAGCGCGCATCTGAAGCAGGT‑AAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAGCCGGGCTAATCA                                               <  SRR4302540.6582156/94‑1 (MQ=60)
                                               TAATTTCAAGAATGACCCGCAGCGCGCATCTGAAGCAGGT‑AAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAGCCGGGCTAATCAA                                              <  SRR4302540.9504939/94‑1 (MQ=60)
                                                AATTTCAAGAATGACCCGCAGCGCGCATCTGAAGCAGGT‑AAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAGCCGGGCTAATCAAT                                             <  SRR4302540.8124574/94‑1 (MQ=60)
                                                AATTTCAAGAATGACCCGCAGCGCGCATCTGAAGCAGGT‑AAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAGCCGGGCTAATCAAT                                             <  SRR4302540.4463696/94‑1 (MQ=60)
                                                AATTTCAAGAATGACCCGCAGCGCGCATCTGAAGCAGGT‑AAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAGCCGGGCTAATCAAT                                             <  SRR4302540.10101125/94‑1 (MQ=60)
                                                AATTTCAAGAATGACCCGCAGCGCGCATCTGAAGCAGGT‑AAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAGCCGGGCTAATCAAT                                             >  SRR4302540.3811301/1‑94 (MQ=60)
                                                 ATTTCAAGAATGACCCGCAGCGCGCATCTGAAGCAGGT‑AAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAGCCGGGCTAATCAATG                                            >  SRR4302540.1505490/1‑94 (MQ=60)
                                                 ATTTCAAGAATGACCCGCAGCGCGCATCTGAAGCAGGT‑AAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAGCCGGGCTAATCAATG                                            >  SRR4302540.3584709/1‑94 (MQ=60)
                                                 ATTTCAAGAATGACCCGCAGCGCGCATCTGAAGCAGGT‑AAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAGCCGGGCTAATCAATG                                            <  SRR4302540.612956/94‑1 (MQ=60)
                                                 ATTTCAAGAATGACCCGCAGCGCGCATCTGAAGCAGGT‑AAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAGCCGGGCTAATCAATG                                            <  SRR4302540.5358213/94‑1 (MQ=60)
                                                   TTCAAGAATGACCCGCAGCGCGCATCTGAAGCAGGT‑AAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAGCCGGGCTAATCAATGAC                                          >  SRR4302540.9339563/1‑94 (MQ=60)
                                                   TTCAAGAATGACCCGCAGCGCGCATCTGAAGCAGGT‑AAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAGCCGGGCTAATCAATGAC                                          >  SRR4302540.3437401/1‑94 (MQ=60)
                                                   TTCAAGAATGACCCGCAGCGCGCATCTGAAGCAGGT‑AAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAGCCGGGCTAATCAATGAC                                          <  SRR4302540.9528391/94‑1 (MQ=60)
                                                    TCAAGAATGACCCGCAGCGCGCATCTGAAGCAGGT‑AAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAGCCGGGCTAATCAATGACG                                         <  SRR4302540.4129925/94‑1 (MQ=60)
                                                    TCAAGAATGACCCGCAGCGCGCATCTGAAGCAGGT‑AAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAGCCGGGCTAATCAATGACG                                         <  SRR4302540.190410/94‑1 (MQ=60)
                                                       AGAATGACCCGCAGCGCGCATCTGAAGCAGGT‑AAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAGCCGGGCTAATCAATGACGAAT                                      <  SRR4302540.5532996/94‑1 (MQ=60)
                                                       AGAATGACCCGCAGCGCGCATCTGAAGCAGGT‑AAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAGCCGGGCTAATCAATGACGAAT                                      <  SRR4302540.5533000/94‑1 (MQ=60)
                                                        GAATGACCCGCAGCGCGCATCTGAAGCAGGT‑AAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAGCCGGGCTAATCAATGACGAATG                                     <  SRR4302540.7960254/94‑1 (MQ=60)
                                                        GAATGACCCGCAGCGCGCATCTGAAGCAGGT‑AAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAGCCGGGCTAATCAATGACGAATG                                     >  SRR4302540.2845648/1‑94 (MQ=60)
                                                         AATGACCCGCAGCGCGCATCTGAAGCAGGT‑AAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAGCCGGGCTAATCAATGACGAATGC                                    >  SRR4302540.9559106/1‑94 (MQ=60)
                                                         AATGACCCGCAGCGCGCATCTGAAGCAGGT‑AAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAGCCGGGCTAATCAATGACGAATGC                                    >  SRR4302540.9559107/1‑94 (MQ=60)
                                                          ATGACCCGCAGCGCGCATCTGAAGCAGGT‑AAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAGCCGGGCTAATCAATGACGAATGCA                                   >  SRR4302540.8201390/1‑94 (MQ=60)
                                                            GACCCGTAGCGCGCATCTGAAGCAGGT‑AAAAAGTTGGTAAGAGCAGTCTCGGCAAAAGCGACAACTAGCCttgctatgtattgacgaatgcatt                                 >  SRR4302540.9090089/1‑70 (MQ=60)
                                                              CCCGCAGCGCGCATCTGAAGCAGGT‑AAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAGCCGGGCTAATCAATGACGAATGCATTTT                               <  SRR4302540.1720424/94‑1 (MQ=60)
                                                               CCGCAGCGCGCATCTGAAGCAGGT‑AAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAGCCGGGCTAATCAATGACGAATGCATTTTT                              <  SRR4302540.6991060/94‑1 (MQ=60)
                                                                CGCAGCGCGCATCTGAAGCAGGT‑AAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAGCCGGGCTAATCAATGACGAATGCATTTTTG                             >  SRR4302540.1884706/1‑94 (MQ=60)
                                                                 GCAGCGCGCATCTGAAGCAGGT‑AAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAGCCGGGCTAATCAATGACGAATGCATTTTTGT                            >  SRR4302540.7124874/1‑94 (MQ=60)
                                                                  CAGCGCGCATCTGAAGCAGGT‑AAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAGCCGGGCTAATCAATGACGAATGCATTTTTGTC                           >  SRR4302540.3086211/1‑94 (MQ=60)
                                                                   AGCGCGCATCTGAAGCAGGT‑AAAAAGGTGTTAAGAGCAGTCACGGCAAAAGCGACAACTAGCCGGGCTAATCAATGACGAATGCATTTTTGTCT                          <  SRR4302540.2214309/94‑1 (MQ=60)
                                                                   AGCGCGCATCTGAAGCAGGT‑AAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAGCCGGGCTAATCAATGACGAATGCATTTTTGTCT                          <  SRR4302540.633997/94‑1 (MQ=60)
                                                                   AGCGCGCATCTGAAGCAGGT‑AAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAGCCGGGCTAATCAATGACGAATGCATTTTTGTCT                          >  SRR4302540.5410034/1‑94 (MQ=60)
                                                                      GCGCATCTGAAGCAGGT‑AAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAGCCGGGCTAATCAATGACGAATGCATTTTTGTCTGTA                       <  SRR4302540.1031544/94‑1 (MQ=60)
                                                                       CGCATCTGAAGCAGGT‑AAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAGCCGGGCTAATCAATGACGAATGCATTTTTGTCTGTAG                      >  SRR4302540.3071687/1‑94 (MQ=60)
                                                                        GCATCTGAAGCAGGT‑AAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAGCCGGGCTAATCAATGACGAATGCATTTTTGTCTGTAGC                     >  SRR4302540.8168716/1‑94 (MQ=60)
                                                                        GCATCTGAAGCAGGT‑AAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAGCCGGGCTAATCAATGACGAATGCATTTTTGTCTGTAGC                     <  SRR4302540.6977836/94‑1 (MQ=60)
                                                                        GCATCTGAAGCAGGT‑AAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAGCCGGGCTAATCAATGACGAATGCATTTTTGTCTGTAGC                     >  SRR4302540.5759645/1‑94 (MQ=60)
                                                                         CATCTGAAGCAGGT‑AAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAGCCGGGCTAATCAATGACGAATGCATTTTTGTCTGTAGCT                    >  SRR4302540.4728869/1‑94 (MQ=60)
                                                                         CATCTGAAGCAGGT‑AAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAGCCGGGCTAATCAATGACGAATGCATTTTTGTCTGTAGCT                    <  SRR4302540.5184125/94‑1 (MQ=60)
                                                                         CATCTGAAGCAGGT‑AAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAGCCGGGCTAATCAATGACGAATGCATTTTTGTCTGTAGCT                    >  SRR4302540.7832155/1‑94 (MQ=60)
                                                                         CATCTGAAGCAGGT‑AAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAGCCGGGCTAATCAATGACGAATGCATTTTTGTCTGTAGCC                    >  SRR4302540.404249/1‑94 (MQ=60)
                                                                          ATCTGAAGCAGGT‑AAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAGCCGGGCTAATCAATGACGAATGCATTTTTGTCTGTAGCTC                   >  SRR4302540.5957039/1‑94 (MQ=60)
                                                                          ATCTGAAGCAGGT‑AAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAGCCGGGCTAATCAATGACGAATGCATTTTTGTCTGTAGCTC                   >  SRR4302540.5576151/1‑94 (MQ=60)
                                                                          ATCTGAAGCAGGT‑AAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAGCCGGGCTAATCAATGACGAATGCATTTTTGTCTGTAGCTC                   >  SRR4302540.573156/1‑94 (MQ=60)
                                                                          ATCTGAAGCAGGT‑AAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAGCCGGGCTAATCAATGACGAATGCATTTTTGTCTGTAGCTC                   <  SRR4302540.6936194/94‑1 (MQ=60)
                                                                            CTGAAGCAGGT‑AAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAGCCGGGCTAATCAATGACGAATGCATTTTTGTCTGTAGCTCGT                 >  SRR4302540.1188872/1‑94 (MQ=60)
                                                                            CTGAAGCAGGT‑AAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAGCCGGGCTAATCAATGACGAATGCATTTTTGTCTGTAGCTCGT                 >  SRR4302540.9398139/1‑94 (MQ=60)
                                                                             TGAAGCAGGT‑AAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAGCCGGGCTAATCAATGACGAATGCATTTTTGTCTGTAGCTCGTC                <  SRR4302540.6086631/94‑1 (MQ=60)
                                                                             TGAAGCAGGT‑AAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAGCCGGGCTAATCAATGACGAATGCATTTTTGTCTGTAGCTCGTA                >  SRR4302540.2531904/1‑94 (MQ=60)
                                                                              GAAGCAGGT‑AAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAGCCGGGCTAATCAATGACGAATGCATTTTTGTCTGTAGCTCGTCA               >  SRR4302540.831978/1‑94 (MQ=60)
                                                                              GAAGCAGGT‑AAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAGCCGGGCTAATCAATGACGAATGCATTTTTGTCTGTAGCTCGTCA               <  SRR4302540.8101968/94‑1 (MQ=60)
                                                                              GAAGCAGGT‑AAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAGCCGGGCTAATCAATGACGAATGCATTTTTGTCTGTAGCTCGTCA               <  SRR4302540.408012/94‑1 (MQ=60)
                                                                              GAAGCAGGT‑AAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAGCCGGGCTAATCAATGACGAATGCATTTTTGTCTGTAGCTCGTCA               <  SRR4302540.7498081/94‑1 (MQ=60)
                                                                               AAGCAGGT‑AAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAGCCGGGCTAATCAATGACGAATGCATTTTTGTCTGTAGCTCGTCAA              >  SRR4302540.6933305/1‑94 (MQ=60)
                                                                                AGCAGGT‑AAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAGCCGGGCTAATCAATGACGAATGCATTTTTGTCTGTAGCTCGTCAAA             <  SRR4302540.1319448/94‑1 (MQ=60)
                                                                                 GCAGGT‑AAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAGCCGGGCTAATCAATGACGAATGCATTTTTGTCTGTAGCTCGTCAAAA            >  SRR4302540.5874657/1‑94 (MQ=60)
                                                                                 GCAGGT‑AAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAGCCGGGCTAATCAATGACGAATGCATTTTTGTCTGTAGCTCGTCAAAA            <  SRR4302540.686768/94‑1 (MQ=60)
                                                                                  CAGGT‑AAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAGCCGGGCTAATCAATGACGAATGCATTTTTGTCTGTAGCTCGTCAAAAA           >  SRR4302540.2061060/1‑94 (MQ=60)
                                                                                  CAGGT‑AAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAGCCGGGCTAATCAATGACGAATGCATTTTTGTCTGTAGCTCGTCAAAAA           >  SRR4302540.1345877/1‑94 (MQ=60)
                                                                                  CAGGT‑AAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAGCCGGGCTAATCAATGACGAATGCATTTTTGTCTGTAGCTCGTCAAAAA           >  SRR4302540.3245237/1‑94 (MQ=60)
                                                                                    GGT‑AAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAGCCGGGCTAATCAATGACGAATGCATTTTTGTCTGTAGCTCGTCAAAAAGC         >  SRR4302540.9200860/1‑94 (MQ=60)
                                                                                    GGT‑AAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAGCCGGGCTAATCAATGACGAATGCATTTTTGTCTGTAGCTCGTCAAAAAGC         >  SRR4302540.7339597/1‑94 (MQ=60)
                                                                                    GGT‑AAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAGCCGGGCTAATCAATGACGAATGCATTTTTGTCTGTAGCTCGTCAAAAAGC         >  SRR4302540.3669623/1‑94 (MQ=60)
                                                                                      gtAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAGCCGGGCTAATCAATGACGAATGCATTTTTGTCTGTAGCTCGTCAAAAAGCC        <  SRR4302540.9184533/92‑1 (MQ=60)
                                                                                       TAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAGCCGGGCTAATCAATGACGAATGCATTTTTGTCTGTAGCTCGTCAAAAAGCCA       >  SRR4302540.6286304/1‑94 (MQ=60)
                                                                                       TAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAGCCGGGCTAATCAATGACGAATGCATTTTTGTCTGTAGCTCGTCAAAAAGCCA       >  SRR4302540.8040120/1‑94 (MQ=60)
                                                                                         AAAAGGTGGTAAGAGCAGTCACGGCATAAGCGACACCTAGCCGGGCTAATCAATGACGAATGCATTTTTGTCTTTAGCTCGTCAAAAAGCCATC     <  SRR4302540.437878/94‑1 (MQ=60)
                                                                                         AAAAGGTGGTAAGAGCAGTCACGGCAAAAGCTACAACTAGCCGGGCTAATCAATGACGAATGCATTTTTGTCTGTAGCTCGTCAAAAAGCCATC     <  SRR4302540.437879/94‑1 (MQ=60)
                                                                                         AAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAGCCGGGCTAATCAATGACGAATGCATTTTTGTCTGTAGCTCGTCAAAAAGCCATC     <  SRR4302540.7464309/94‑1 (MQ=60)
                                                                                          AAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAGCCGGGCTAATCAATGACGAATGCATTTTTGTCTGTAGCTCGTCAAAAAGCCATCA    <  SRR4302540.4682281/94‑1 (MQ=60)
                                                                                           AAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAGCCGGGCTAATCAATGACGAATGCATTTTTGTCTGTAGCTCGTCAAAAAGCCATCAC   <  SRR4302540.7033245/94‑1 (MQ=60)
                                                                                            AGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAGCCGGGCTAATCAATGACGAATGCATTTTTGTCTGTAGCTCGTCAAAAAGCCATCACC  <  SRR4302540.5884307/94‑1 (MQ=60)
                                                                                            |                                                                                             
GAAAGAGCATCAGAAGCAGGTAAAAAAGGTGGACAGCACAGCGGGGGTAATTTCAAGAATGACCCGCAGCGCGCATCTGAAGCAGGTAAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAGCCGGGCTAATCAATGACGAATGCATTTTTGTCTGTAGCTCGTCAAAAAGCCATCACC  >  NC_000913/1067349‑1067534

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑