Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 1548 369.5 20095359 96.9% 19472402 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,138,203 A→G Q201Q (CAA→CAG)  flgK → hook‑filament junction protein 1

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,138,2030AG100.0% 710.7 / NA 217Q201Q (CAA→CAG) flgKhook‑filament junction protein 1
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (88/129);  total (88/129)

Only 100 of 229 total aligned reads displayed.

TACGCTAAACAAATTGCCAGCCTGAACGATCAAATCTCGCGCCTGACAGGCGTGGGGGCAGGGGCGTCACCTAACAATCTGCTGGATCAACGCGATCAACTGGTGAGCGAATTAAACCAGATTGTTGGTGTAGAAGTCAGCGTTCAGGATGGCGGCACTTATAACATCACGATGGCCAA  >  NC_000913/1138114‑1138292
                                                                                         |                                                                                         
tACGCTAAACAAATTGCCAGCCTGAACGATCAAATCTCGCGCCTGACAGGCGTGGGGGCAGGGGCGTCACCTAACAATCTGCTGGATCAgcgc                                                                                        <  1:1690474/93‑1 (MQ=255)
tACGCTAAACAAATTGCCAGCCTGAACGATCAAATCTCGCGCCTGACAGGCGTGGGGGCAGGGGCGTCACCTAACAATCTGCTGGATCAgcgc                                                                                        >  1:6324307/1‑93 (MQ=255)
tACGCTAAACAAATTGCCAGCCTGAACGATCAAATCTCGCGCCTGACAGGCGTGGGGGCAGGGGCGTCACCTAACAATCTGCTGGATCAgcgc                                                                                        >  1:4445692/1‑93 (MQ=255)
tACGCTAAACAAAATGCCAGCCTGAACGATCAAATCTCGCGCCTGACAGGCGTGGGGGCAGGGGCGTCACCTAACAATCTGCTGGATCAgcgc                                                                                        >  1:5714626/1‑93 (MQ=255)
 aCGCTAAACAAATTGCCAGCCTGAACGATCAAATCTCGCGCCTGACAGGCGTGGGGGCAGGGGCGTCACCTAACAATCTGCTGGATCAGcgcg                                                                                       >  1:8256910/1‑93 (MQ=255)
  cGCTAAACAAATTGCCAGCCTGAACGATCAAATCTCGCGCCTGACAGGCGTGGGGGCAGGGGCGTCACCTAACAATCTGCTGGATCAGCGCGa                                                                                      >  2:8711366/1‑93 (MQ=255)
   gCTAAACAAATTGCCAGCCTGAACGATCAAATCTCGCGCCTGACAGGCGTGGGGGCAGGGGCGTCACCTAACAATCTGCTGGATCAGCGCGAt                                                                                     >  1:8772908/1‑93 (MQ=255)
    cTAAACAAATTGCCAGCCTGAACGATCAAATCTCGCGCCTGACAGGCGTGGGGGCAGGGGCGTCACCTAACAATCTGCTGGATCAGCGCGATc                                                                                    <  1:4005896/93‑1 (MQ=255)
     tAAACAAATTGCCAGCCTGAACGATCAAATCTCGCGCCTGACAGGCGTGGGGGCAGGGGCGTCACCTAACAATCTGCTGGATCAGCGCGATCa                                                                                   >  2:1623543/1‑93 (MQ=255)
      aaaCAAATTGCCAGCCTGAACGATCAAATCTCGCGCCTGACAGGCGTGGGGGCAGGGGCGTCACCTAACAATCTGCTGGATCAGCGCGATCaa                                                                                  <  2:401255/93‑1 (MQ=255)
      aaaCAAATTGCCAGCCTGAACGATCAAATCTCGCGCCTGACAGGCGTGGGGGCAGGGGCGTCACCTAACAATCTGCTGGATCAGCCCGATCaa                                                                                  >  1:9817750/1‑93 (MQ=255)
       aaCAAATTGCCAGCCTGAACGATCAAATCTCGCGCCTGACAGGCGTGGGGGCAGGGGCGTCACCTAACAATCTGCTGGATCAGCGCGATCAAc                                                                                 <  1:3315128/93‑1 (MQ=255)
        aCAAATTGCCAGCCTGAACGATCAAATCTCGCGCCTGACAGGCGTGGGGGCAGGGGCGTCACCTAACAATCTGCTGGATCAGCGCGATCAACt                                                                                <  1:2442738/93‑1 (MQ=255)
        aCAAATTGCCAGCCTGAACGATCAAATCTCGCGCCTGACAGGCGTGGGGGCAGGGGCGTCACCTAACAATCTGCTGGATCAGCGCGATCAACt                                                                                >  2:6398337/1‑93 (MQ=255)
           aaTTGCCAGCCTGAACGATCAAATCTCGCGCCTGACAGGCGTGGGGGCAGGGGCGTCACCTAACAATCTGCTGGATCAGCGCGATCAACTGGt                                                                             >  2:7747988/1‑93 (MQ=255)
            aTTGCCAGCCTGAACGATCAAATCTCGCGCCTGACAGGCGTGGGGGCAGGGGCGTCACCTAACAATCTGCTGGATCAGCGCGATCAACTGGTg                                                                            >  1:8683696/1‑93 (MQ=255)
            aTTGCCAGCCTGAACGATCAAATCTCGCGCCTGACAGGCGTGGGGGCAGGGGCGTCACCTAACAATCTGCTGGATCAGCGCGATCAACTGGTg                                                                            >  2:4794035/1‑93 (MQ=255)
             ttGCCAGCCTGAACGATCAAATCTCGCGCCTGACAGGCGTGGGGGCAGGGGCGTCACCTAACAATCTGCTGGATCAGCGCGATCAACTGGTGa                                                                           <  1:2097928/93‑1 (MQ=255)
             ttGCCAGCCTGAACGATCAAATCTCGCGCCTGACAGGCGTGGGGGCAGGGGCGTCACCTAACAATCTGCTGGATCAGCGCGATCAACTGGTGa                                                                           >  2:960791/1‑93 (MQ=255)
             ttGCCAGCCTGAACGATCAAATCTCGCGCCTGACAGGCGTGGGGGCAGGGGCGTCACATAACAATCTGCTGGATCAGCGCGATCAACTGGTGa                                                                           <  2:7724878/93‑1 (MQ=255)
              tGCCAGCCTGAACGATCAAATCTCGCGCCTGACAGGCGTGGGGGCAGGGGCGTCACCTAACAATCTGCTGGATCAGCGCGATCAACTGGTGAg                                                                          <  2:3505783/93‑1 (MQ=255)
              tGCCAGCCTGAACGATCAAATCTCGCGCCTGACAGGCGTGGGGGCAGGGGCGTCACCTAACAATCTGCTGGATCAGCGCGATCAACTGGTGAg                                                                          <  2:7480980/93‑1 (MQ=255)
               gCCAGCCTGAACGATCAAATCTCGCGCCTGACAGGCGTGGGGGCAGGGGCGTCACCTAACAATCTGCTGGATCAGCGCGATCAACTGGTGAGc                                                                         <  2:816008/93‑1 (MQ=255)
                   gCCTGAACGATCAAATCTCGCGCCTGACAGGCGTGGGGGCAGGGGCGTCACCTAACAATCTGCTGGATCAGCGCGATCAACTGGTGAGCGAAt                                                                     >  1:9706180/1‑93 (MQ=255)
                    ccTGAACGATCAAATCTCGCGCCTGACAGGCGTGGGGGCAGGGGCGTCACCTAACAATCTGCTGGATCAGCGCGATCAACTGGTGAGCGAAtt                                                                    <  1:705950/93‑1 (MQ=255)
                    ccTGAACGATCAAATCTCGCGCCTGACAGGCGTGGGGGCAGGGGCGTCACCTAACAATCTGCTGGATCAGCGCGATCAACTGGTGAGCGAAtt                                                                    <  2:97371/93‑1 (MQ=255)
                     cTGAACGATCAAATCTCGCGCCTGACAGGCGTGGGGGCAGGGGCGTCACCTAACAATCTGCTGGATCAGCGCGATCAACTGGTGAGCGAATTa                                                                   <  2:5753950/93‑1 (MQ=255)
                       gAACGATCAAATCTCGCGCCTGACAGGCGTGGGGGCAGGGGCGTCACCTAACAATCTGCTGGATCAGCGCGATCAACTGGTGAGCGAATTaaa                                                                 >  1:9003004/1‑93 (MQ=255)
                        aaCGATCAAATCTCGCGCCTGACAGGCGTGGGGGCAGGGGCGTCACCTAACAATCTGCTGGATCAGCGCGATCAACTGGTGAGCGAATTAAAc                                                                <  1:7922389/93‑1 (MQ=255)
                         aCGATCAAATCTCGCGCCTGACAGGCGTGGGGGCAGGGGCGTCACCTAACAATCTGCTGGATCAGCGCGATCAACTGGTGAGCGAACTAAAcc                                                               <  2:9879899/93‑1 (MQ=255)
                          cGATCAAATCTCGCGCCTGACAGGCGTGGGGGCAGGGGCGTCACCTAACAATCTGCTGGATCAGCGCGATCAACTGGTGAGCGAATTAAACCa                                                              >  2:3734229/1‑93 (MQ=255)
                           gATCAAATCTCGCGCCTGACAGGCGTGGGGGCAGGGGCGTCACCTAACAATCTGCTGGATCAGCGCGATCAACTGGTGAGCGAATTAAACCAg                                                             <  2:234792/93‑1 (MQ=255)
                                aaTCTCGCGCCTGACAGGCGTGGGGGCAGGGGCGTCACCTAACAATCTGCTGGATCAGCGCGATCAACTGGTGAGCGAATTAAACCAGAttgt                                                        <  2:4534458/93‑1 (MQ=255)
                                 aTCTCGCGCCTGACAGGCGTGGGGGCAGGGGCGTCACCTAACAATCTGCTGGATCAGCGCGATCAACTGGTGAGCGAATTAAACCAGAttgtt                                                       <  2:1784572/93‑1 (MQ=255)
                                 aTCTCGCGCCTGACAGGCGTGGGGGCAGGGGCGTCACCTAACAATCTGCTGGATCAGCGCGATCAACTGGTGAGCGAATTAAACCAGAttgtt                                                       >  1:838545/1‑93 (MQ=255)
                                  tctcGCGCCTGACAGGCGTGGGGGCAGGGGCGTCACCTAACAATCTGCTGGATCAGCGCGATCAACTGGTGAGCGAATTAAACCAGAttgttg                                                      <  1:62527/93‑1 (MQ=255)
                                    tcGCGCCTGACAGGCGTGGGGGCAGGGGCGTCACCTAACAATCTGCTGGATCAGCGCGATCAACTGGTGAGCGAATTAAACCAGATTGTTGgt                                                    <  2:9059195/93‑1 (MQ=255)
                                    tcGCGCCTGACAGGCGTGGGGGCAGGGGCGTCACCTAACAATCTGCTGGATCAGCGCGATCAACTGGTGAGCGAATTAAACCAGATTGTTGgt                                                    >  2:5201767/1‑93 (MQ=255)
                                    tcGCGCCTGACAGGCGTGGGGGCAGGGGCGTCACCTAACAATCTGCTGGATCAGCGCGATCAACTGGTGAGCGAATTAAACCAGATTGTTGgt                                                    <  2:7145184/93‑1 (MQ=255)
                                     cgcgCCTGACAGGCGTGGGGGCAGGGGCGTCACCTAACAATCTGCTGGGTCAGCGCGATCAACTGGTGAGCGAATTAAACCAGATTGTTGgtg                                                   <  2:8080032/93‑1 (MQ=255)
                                     cgcgCCTGACAGGCGTGGGGGCAGGGGCGTCACCTAACAATCTGCTGGATCAGCGCGATCAACTGGTGAGCGAATTAAACCAGATTGTTGgtg                                                   <  1:6920063/93‑1 (MQ=255)
                                        gcCTGACAGGCGTGGGGGCAGGGGCGTCACCTAACAATCTGCTGGATCAGCGCGATCAACTGGTGAGCGAATTAAACCAGATTGTTGGTGTAg                                                <  1:75385/93‑1 (MQ=255)
                                         cgtGACAGGCGTGGGGGCAGGGGCGTCACCTAACAATCTGCTGGATCAGCGCGATCAACTGGTGAGCGAATTAAACCAGATTGTTGGTGTAGa                                               <  1:3257652/91‑1 (MQ=255)
                                         ccTGACAGGCGTGGGGGCAGGGGCGTCACCTAACAATCTGCTGGATCAGCGCGATCAACTGGTGAGCGAATTAAACCAGATTGTTGGTGTGGa                                               <  1:1839740/93‑1 (MQ=255)
                                         ccTGACAGGCGTGGGGGCAGGGGCGTCACCTAACAATCTGCTGGATCAGCGCGATCAACTGGTGAGCGAATTAAACCAGATTGTTGGTGTAGa                                               <  1:5127056/93‑1 (MQ=255)
                                         ccTGACAGGCGTGGGGGCAGGGGCGTCACCTAACAATCTGCTGGATCAGCGCGATCAACTGGTGAGCGAATTAAACCAGATTGTTGGTGTAGa                                               <  1:5178955/93‑1 (MQ=255)
                                         ccTGACAGGCGTGGGGGCAGGGGCGTCACCTAACAATCTGCTGGATCAGCGCGATCAACTGGTGAGCGAATTAAACCAGATTGTTGGTGTAGa                                               <  2:1343429/93‑1 (MQ=255)
                                          cTGACAGGCGTGGGGGCAGGGGCGTCACCTAACAATCTGCTGGATCAGCGCGATCAACTGGTGAGCGAATTAAACCAGATTGTTGGTGTAGaa                                              >  1:5243917/1‑93 (MQ=255)
                                           tGACAGGCGTGGGGGCAGGGGCGTCACCTAACAATCTGCTGGATCAGCGCGATCAACTGGTGAGCGAATTAAACCAGATTGTTGGTGTAGAAg                                             <  2:4931203/93‑1 (MQ=255)
                                            gACAGGCGTGGGGGCAGGGGCGTCACCTAACAATCTGCTGGCTCAGCGCGATCAACTGGTGAGCGAATTAAACCAGATTGTTGGGGTAGAAgc                                            >  1:3179786/1‑92 (MQ=255)
                                            gACAGGCGTGGGGGCAGGGGCGTCACCTAACAATCTGCTGGATCAGCGCGATCAACTGGTGAGCGAATTAAACCAGATTGTTGGTGTAGAAGt                                            >  1:8012633/1‑93 (MQ=255)
                                             aCAGGCGTGGGGGCAGGGGCGTCACCTAACAATCTGCTGGATCAGCGCGATCAACTGGTGAGCGAATTAAACCAGATTGTTGGTGTAGAAGTc                                           <  1:6544024/93‑1 (MQ=255)
                                              cAGGCGTGGGGGCAGGGGCGTCACCTAACAATCTGCTGGATCAGCGCGATCAACTGGTGAGCGAATTAAACCAGATTGTTGGTGTAGAAGTCa                                          <  2:2260148/93‑1 (MQ=255)
                                              cAGGCGTGGGGGCAGGGGCGTCACCTAACAATCTGCTGGATCAGCGCGATCAACTGGTGAGCGAATTAAACCAGATTGTTGGTGTAGAAGTCa                                          <  2:8884503/93‑1 (MQ=255)
                                              aaGGCGTGGGGGCAGGGGCGTCACCTAACAATCTGCTGGATCAGCGCGATCAACTGGTGAGCGAATTAAACCAGATTGTTGGTGTAGAAGTCa                                          <  1:8298361/92‑1 (MQ=255)
                                                  cGTGGGGGCAGGGGCGTCACCTAACAATCTGCTGGATCAGCGCGATCAACTGGTGAGCGAATTAAACCAGATTGTTGGTGTAGAAGTCAGCGt                                      <  1:5400198/93‑1 (MQ=255)
                                                    tGGGGGCAGGGGCGTCACCTAACAATCTGCTGGATCAGCGCGATCAACTGGTGAGCGAATTAAACCAGATTGTTGGTGTAGAAGTCAGCGTTc                                    <  1:3681454/93‑1 (MQ=255)
                                                    tGGGGGCAGGGGCGTCACCTAACAATCTGCTGGATCAGCGCGATCAACTGGTGAGCGAATTAAACCAGATTGTTGGTGTAGAAGTCAGCGTTc                                    >  2:2864161/1‑93 (MQ=255)
                                                     gggggCAGGGGCGTCACCTAACAATCTGCTGGATCAGCGCGATCAACTGGTGAGCGAATTAAACCAGATTGTTGGTGTAGAAGTCAGCGTTCa                                   <  2:8345301/93‑1 (MQ=255)
                                                     gggggCAGGGGCGTCACCTAACAATCTGCTGGATCAGCGCGATCAACTGGTGAGCGAATTAAACCAGATTGTTGGTGTAGAAGTCAGCGTTCa                                   <  1:3412604/93‑1 (MQ=255)
                                                        ggCAGGGGCGTCACCTAACAATCTGCTGGATCAGCGCGATCAACTGGTGAGCGAATTAAACCAGATTGTTGGTGTAGAAGTCAGCGTTCAGGa                                <  1:5646529/93‑1 (MQ=255)
                                                         gCAGGGGCGTCACCTAACAATCTGCTGGATCAGCGCGATCAACTGGTGAGCGAATTAAACCAGATTGTTGGTGTAGAAGTCAGCGTTCAGGAt                               <  1:4774499/93‑1 (MQ=255)
                                                          cAGGGGCGTCACCGAACAGTCGGCTGGATCAGCGCGATCAACTGGTGAGCGAATTAAACCAGATTGTTGGTGTAGAAGTCAGCGGTCAGGATg                              <  2:4139451/93‑1 (MQ=255)
                                                           aGGGGCGTCACCTAACAATCTGCTGGATCAGCGCGATCAACTGGTGAGCGAATTAAACCAGATTGTTGGTGTAGAAGTCAGCGTTCAGGATgg                             >  1:5768558/1‑93 (MQ=255)
                                                            gggtCGCCACTTAACATTCTGCTGGATCAGCGCAATCAACTGGTGAGCAAATAAACCCAGTTTTTTGGTGTAGAAGTCAGCGTTCAGGATggc                            <  2:27519/93‑1 (MQ=255)
                                                            ggggCGTCACCTAACAATCTGCTGGATCAGCGCGATCAACTGGTGAGCGAATTAAACCAGATTGTTGGTGTAGAAGTCAGCGTTCAGGATggc                            >  2:1067014/1‑93 (MQ=255)
                                                            ggggCGTCACCTAACAATCTGCTGGATCAGCGCGATCAACTGGTGAGCGAATTAAACCAGATTGTTGGTGTAGAAGTCAGAGTTCAGGATggc                            >  2:6725898/1‑93 (MQ=255)
                                                             gggCGTCACCTAACAATCTGCTGGATCAGCGCGATCAACTGGTGAGCGAATTAAACCAGATTGTTGGTGTAGAAGTCAGCGTTCAGGATggcg                           <  2:5882360/93‑1 (MQ=255)
                                                             gggCGTCACCTAACAATCTGCTGGATCAGCGCGATCAACTGGTGAGCGAATTAAACCAGATTGTTGGTGTAGAAGTCAGCGTTCAGGATggcg                           >  1:7238223/1‑93 (MQ=255)
                                                              gacGTCACCTAACAAGCTGCTGGATCAGCGCGATCAACTGGTGAGCGAATTAAACCAGATTGTTGGTGTAGAAGTCAGCGTTCAGGATggcgg                          <  1:7428567/91‑1 (MQ=255)
                                                               gCGTCACCTAACAATCTGCTGGATCAGCGCGATCAACTGGTGAGCGAATTAAACCAGATTGTTGGTGTAGAAGTCAGCGTTCAGGATggcggc                         <  1:6708024/93‑1 (MQ=255)
                                                                cGTCACCTAACAATCTGCTGGATCAGCGCGATCAACTGGTGAGCGAATTAAACCAGATTGTTGGTGTAGAAGTCAGCGTTCAGGATGGCGGCa                        <  1:7723684/93‑1 (MQ=255)
                                                                cGTCACCTAACAATCTGCTGGATCAGCGCGATCAACTGGTGAGCGAATTAAACCAGATTGTTGGTGTAGAAGTCAGCGTTCAGGATGGCGGCa                        >  1:417244/1‑93 (MQ=255)
                                                                 gTCACCTAACAATCTGCTGGATCAGCGCGATCAACTGGTGAGCGAATTAAACCAGATTGTTGGTGTAGAAGTCAGCGTTCAGGATGGCGGCAc                       >  1:3032310/1‑93 (MQ=255)
                                                                  tCACCTAACAATCTGCTGGATCAGCGGGATCAACTGGTGAGCAAATTAAACCAGATTGTTGGTGTAGAAGTCAGCGTTCAGGATGGCGGCACt                      <  2:3949782/93‑1 (MQ=255)
                                                                  tCACCTAACAATCTGCTGGATCAGCGCGATCAACTGGTGAGCGAATTAAACCAGATTGTTGGTGTAGAAGTCAGCGTTCAGGATGGCGGCACt                      >  1:3805385/1‑93 (MQ=255)
                                                                    cccTAACAATCTGCTGGATCAGCGCGATCAACTGGTGAGCGAATTAAACCAGATTGTTGGTGTAGAAGTCAGCGTTCAGGATGGCGGCACTta                    <  2:1911515/92‑1 (MQ=255)
                                                                    aCCTAACAATCTGCTGGATCAGCGCGATCAACTGGTGAGCGAATTAAACCAGATTGTTGGTGTAGAAGTCAGCGTTCAGGATGGCGGCACTta                    <  1:4737777/93‑1 (MQ=255)
                                                                      cTAACAATCTGCTGGATCAGCGCGATCAACTGGTGAGCGAATTAAACCAGATTGTTGGTGTAGAAGTCAGCGTTCAGGATGGCGGCACTtata                  >  2:3197551/1‑93 (MQ=255)
                                                                      cTAACAATCTGCTGGATCAGCGCGATCAACTGGTGAGCGAATTAAACCAGATTGTTGGTGTAGAAGTCAGCGTTCAGGATGGCGGCACTtata                  <  2:783893/93‑1 (MQ=255)
                                                                       tAACAATCTGCTGGATCAGCGCGATCAACTGGTGAGCGAATTAAACCAGATTGTTGGTGTAGAAGTCAGCGTTCAGGATGGCGGCACTTATaa                 <  2:5155097/93‑1 (MQ=255)
                                                                       tAACAATCTGCTGGATCAGCGCGATCAACTGGTGAGCGAATTAAACCAGATTGTTGGTGTAGAAGTCAGCGTTCAGGATGGCGGCACTTATaa                 <  1:2346508/93‑1 (MQ=255)
                                                                         aCAATCTGCTGGATCAGCGCGATCAACTGGTGAGCGAATTAAACCAGATTGTTGGTGTAGAAGTCAGCGTTCAGGATGGCGGCACTTATAACa               <  1:1036818/93‑1 (MQ=255)
                                                                            aTCTGCTGGATCAGCGCGATCAACTGGTGAGCGAATTAAACCAGATTGTTGGTGTAGAAGTCAGCGTTCAGGATGGCGGCACTTATAACATCa            <  1:2568967/93‑1 (MQ=255)
                                                                            aTCTGCTGGATCAGCGCGATCAACTGGTGAGCGAATTAAACCAGATTGTTGGTGTAGAAGTCAGCGTTCAGGATGGCGGCACTTATAACATCa            >  1:8509309/1‑93 (MQ=255)
                                                                            aTCTGCTGGATCAGCGCGATCAACTGGTGAGCGAATTAAACCAGATTGTTGGTGTAGAAGTCAGCGTTCAGGATGGCGGCACTTATAACATCa            <  2:6517409/93‑1 (MQ=255)
                                                                            aTCTGCTGGATCAGCGCGATCAACTGGTGAGCGAATTAAACCAGATTGTTGGTGTAGAAGTCAGCGTTCAGGATGGCGGCACTTATAACATCa            <  2:8524518/93‑1 (MQ=255)
                                                                            aTCTGCTGGATCAGCGCGATCAACTGGTGAGCGAATTAAACCAGATTGTTGGTGTAGAAGTCAGCGTTCAGGATGGCGGCACTTATAACATCa            <  2:5579868/93‑1 (MQ=255)
                                                                             tCTGCTGGATCAGCGCGATCAACTGGTGAGCGAATTAAACCAGATTGTTGGTGTAGAAGTCAGCGTTCAGGATGGCGGCACTTATAACATCAc           <  2:6602976/93‑1 (MQ=255)
                                                                             tCTGCTGGATCAGCGCGATCAACTGGTGAGCGAATTAAACCAGATTGTTGGTGTAGAAGTCAGCGTTCAGGATGGCGGCACTTATAACATCAc           <  2:368221/93‑1 (MQ=255)
                                                                               tgctgGATCAGCGCGATCAACTGGTGAGCGAATTAAACCAGATTGTTGGTGTAGAAGTCAGCGTTCAGGGTGGCGGCACTTATAACATCACGa         <  2:5563858/93‑1 (MQ=255)
                                                                               tgctgGATCAGCGCGATCAACTGGTGAGCGAATTAAACCAGATTGTTGGTGTAGAAGTCAGCGTTCAGGATGGCGGCACTTATAACATCACGa         <  2:7051424/93‑1 (MQ=255)
                                                                               tgctgGATCAGCGCGATCAACTGGTGAGCGAATTAAACCAGATTGTTGGTGTAGAAGTCAGCGTTCAGGATGGCGGCACTTATAACATCACGa         <  2:4843909/93‑1 (MQ=255)
                                                                                gctgGATCAGCGCGATCAACTGGTGAGCGAATTAAACCAGATTGTTGGTGTAGAAGTCAGCGTTCAGGATGGCGGCACTTATAACATCACGAt        >  2:5254132/1‑93 (MQ=255)
                                                                                 ctgGATCAGCGCGATCAACTGGTGAGCGAATTAAACCAGATTGTTGGTGTAGAAGTCAGCGTTCAGGATGGCGGCACTTATAACATCACGATg       <  1:6498587/93‑1 (MQ=255)
                                                                                  tgGATCAGCGCGATCAACTGGTGAGCGAATTAAACCAGATTGTTGGTGTAGAAGTCAGCGTTCAGGATGGCGGCACTTATAACATCACGATgg      >  1:9588265/1‑93 (MQ=255)
                                                                                   ggATCAGCGCGATCAACTGGTGAGCGAATTAAACCAGATTGTTGGTGTAGAAGTCAGCGTTCAGGATGGCGGCACTTATAACATCACGATGGc     >  1:3563690/1‑93 (MQ=255)
                                                                                    gATCAGCGCGATCAACTGGTGAGCGAATTAAACCAGATTGTTGGTGTAGAAGTCAGCGTTCAGGATGGCGGCACTTATAACATCACGATGGcc    >  1:3605162/1‑93 (MQ=255)
                                                                                    gATCAGCGCGATCAACTGGTGAGCGAATTAAACCAGATTGTTGGTGTAGAAGTCAGCGTTCAGGATGGCGGCACTTATAACATCACGATGGcc    >  2:2691436/1‑93 (MQ=255)
                                                                                      tCAGCGCGATCAACTGGTGAGCGAATTAAACCAGATTGTTGGTGTAGAAGTCAGCGTTCAGGATGGCGGCACTTATAACATCACGATGGCCaa  >  2:2092224/1‑93 (MQ=255)
                                                                                         |                                                                                         
TACGCTAAACAAATTGCCAGCCTGAACGATCAAATCTCGCGCCTGACAGGCGTGGGGGCAGGGGCGTCACCTAACAATCTGCTGGATCAACGCGATCAACTGGTGAGCGAATTAAACCAGATTGTTGGTGTAGAAGTCAGCGTTCAGGATGGCGGCACTTATAACATCACGATGGCCAA  >  NC_000913/1138114‑1138292

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CAACTACGCTAAACAAATTGCCAGCCTGAACGATCAAATCTCGCGCCTGACAGGCGTGGGGGCAGGGGCGTCACCTAACAATCTGCTGGATCAACGCGATCAACTGGTGAGCGAATTAAACCAGATTGTTGGTGTAGAAGTCAGCGTTCAGGATGGCGGCACTTATAACATCACGATGGCCAATGGT  >  NC_000913/1138110‑1138296
                                                                                             |                                                                                             
CAACTACGCTAAACAAAGTGCCAGCCTGAACGATCAAATCTCGCGCCTGACAGGCGTGGGGGCAGGGGCGTCACCTAACAATCTGCTGGATCAG                                                                                               <  SRR4302540.9168973/94‑1 (MQ=60)
 AACTACGCTAAACAAATTGCCAGCCTGAACGATCAAATCTCGCGCCTGACAGGCGTGGGGGCAGGGGCGTCACCTAACAATCTGCTGGATCAGC                                                                                              <  SRR4302540.8699535/94‑1 (MQ=60)
 AACTACGCTAAACAAATTGCCAGCCTGAACGATCAAATCTCGCGCCTGACAGGCGTGGGGGCAGGGGCGTCACCTAACAATCTGCTGGATCAGC                                                                                              >  SRR4302540.4041772/1‑94 (MQ=60)
  ACTACGCTAAACAAATTGCCAGCCTGAACGATCAAATCTCGCGCCTGACAGGCGTGGGGGCAGGGGCGTCACCTAACAATCTGCTGGATCAGCG                                                                                             <  SRR4302540.6563535/94‑1 (MQ=60)
  ACTACGCTAAACAAATTGCCAGCCTGAACGATCAAATCTCGCGCCTGACAGGCGTGGGGGCAGGGGCGTCACCTAACAATCTGCTGGATCAGCG                                                                                             <  SRR4302540.5322941/94‑1 (MQ=60)
   CTACGCTAAACAAATTGCCAGCCTGAACGATCAAATCTCGCGCCTGACAGGCGTGGGGGCAGGGGCGTCACCTAACAATCTGCTGGATCAGCGC                                                                                            >  SRR4302540.4493875/1‑94 (MQ=60)
   CTACGCTAAACAAATTGCCAGCCTGAACGATCAAATCTCGCGCCTGACAGGCGTGGGGGCAGGGGCGTCACCTAACAATCTGCTGGATCAGCGC                                                                                            >  SRR4302540.6394737/1‑94 (MQ=60)
   CTACGCTAAACAAAATGCCAGCCTGAACGATCAAATCTCGCTCCTGACAGGCGTGGGGGCAGGGGCGTCACCTAACAATCTGCTGGATCAGCGC                                                                                            >  SRR4302540.5777528/1‑94 (MQ=60)
   CTACGCTAAACAAAATGCCAGCCTGAACGATCAAATCTCGCGCCTGACAGGCGTGGGGGCAGGGGCGTCACCTAACAATCTGCTGGATCAGCGC                                                                                            >  SRR4302540.5777529/1‑94 (MQ=60)
   ATACGCTAAACAAATTGCCAGCCTGAACGATCAAATCTCGCGCCTGACAGGCGTGGGGGCAGGGGCGTCACCTAACAATCTGCTGGATCAGCGC                                                                                            <  SRR4302540.2601150/94‑1 (MQ=60)
    TACGCTAAACAAATTGCCAGCCTGAACGATCAAATCTCGCGCCTGACAGGCGTGGGGGCAGGGGCGTCACCTAACAATCTGCTGGATCAGCGCG                                                                                           >  SRR4302540.8352866/1‑94 (MQ=60)
    TACGCTAAACAAATTGCCAGCCTGAACGATCAAATCTCGCGCCTGACAGGCGTGGGGGCAGGGGCGTCACCTAACAATCTGCTGGATCAGCGCG                                                                                           <  SRR4302540.5344790/94‑1 (MQ=60)
    TACGCTAAACAAATTGCCAGCCTGAACGATCAAATCTCGCGCCTGACAGGCGTGGGGGCAGGGGCGTCACCTAACAATCTGCTGGATCAGCGCG                                                                                           <  SRR4302540.1708807/94‑1 (MQ=60)
      CGCTAAACAAATTGCCAGCCTGAACGATCAAATCTCGCGCCTGACAGGCGTGGGGGCAGGGGCGTCACCTAACAATCTGCTGGATCAGCGCGAT                                                                                         >  SRR4302540.8874551/1‑94 (MQ=60)
        CTAAACAAATTGCCAGCCTGAACGATCAAATCTCGCGCCTGACAGGCGTGGGGGCAGGGGCGTCACCTAACAATCTGCTGGATCAGCGCGATCA                                                                                       <  SRR4302540.4049027/94‑1 (MQ=60)
         TAAACAAATTGCCAGCCTGAACGATCAAATCTCGCGCCTGACAGTCGTGGGGGCAGGGGCGTCACCTAACAATCTGCTGGATCAGCGCGATCAA                                                                                      <  SRR4302540.6021518/94‑1 (MQ=60)
         TAAACAAATTGCCAGCCTGAACGATCAAATCTCGCGCCTGACAGGCGTGGGGGCAGGGGCGTCACCTAACAATCTGCTGGATCAGCGCGATCAA                                                                                      >  SRR4302540.3624599/1‑94 (MQ=60)
         TAAACAAATTGCCAGCCTGAACGATCAAATCTCGCGCCTGACAGGCGTGGGGGCAGGGGCGTCACCTAACAATCTGCTGGATCAGCCCGATCAA                                                                                      >  SRR4302540.9932627/1‑94 (MQ=60)
           AACAAATTGCCAGCCTGAACGATCAAATCTCGCGCCTGACAGGCGTGGGGGCAGGGGCGTCACCTAACAATCTGCTGGATCAGCGCGATCAACT                                                                                    <  SRR4302540.3351471/94‑1 (MQ=60)
            ACAAATTGCCAGCCTGAACGATCAAATCTCGCGCCTGACAGGCGTGGGGGCAGGGGCGTCACCTAACAATCTGCTGGATCAGCGCGATCAACTG                                                                                   <  SRR4302540.2469043/94‑1 (MQ=60)
             CAAATTGCCAGCCTGAACGATCAAATCTCGCGCCTGACAGGCGTGGGGGCAGGGGCGTCACCTAACAATCTGCTGGATCAGCGCGATCAACTGG                                                                                  >  SRR4302540.3732643/1‑94 (MQ=60)
             CAAATTGCCAGCCTGAACGATCAAATCTCGCGCCTGACAGGCGTGGGGGCAGGGGCGTCACCTAACAATCTGCTGGATCAGCGCGATCAACTGG                                                                                  >  SRR4302540.5906967/1‑94 (MQ=60)
               AATTGCCAGCCTGAACGATCAAATCTCGCGCCTGACAGGCGTGGGGGCAGGGGCGTCACCTAACAATCTGCTGGATCAGCGCGATCAACTGGTG                                                                                <  SRR4302540.4260958/94‑1 (MQ=60)
               AATTGCCAGCCTGAACGATCAAATCTCGCGCCTGACAGGCGTGGGGGCAGGGGCGTCACCTAACAATCTGCTGGATCAGCGCGATCAACTGGTG                                                                                >  SRR4302540.8784538/1‑94 (MQ=60)
                ATTGCCAGCCTGAACGATCAAATCTCGCGCCTGACAGGCGTGGGGGCAGGGGCGTCACCTAACAATCTGCTGGATCAGCGCGATCAACTGGTGA                                                                               >  SRR4302540.7593473/1‑94 (MQ=60)
                ATTGCCAGCCTGAACGATCAAATCTCGCGCCTGACAGGCGTGGGGGCAGGGGCGTCACCTAACAATCTGCTGGATCAGCGCGATCAACTGGTGA                                                                               <  SRR4302540.5590544/94‑1 (MQ=60)
                 TTGCCAGCCTGAACGATCAAATCTCGCGCCTGACAGGCGTGGGGGCAGGGGCGTCACCTAACAATCTGCTGGATCAGCGCGATCAACTGGTGAG                                                                              <  SRR4302540.5672258/94‑1 (MQ=60)
                 TTGCCAGCCTGAACGATCAAATCTCGCGCCTGACAGGCGTGGGGGCAGGGGCGTCACCTAACAATCTGCTGGATCAGCGCGATCAACTGGTGAG                                                                              <  SRR4302540.2120455/94‑1 (MQ=60)
                    CCAGCCTGAACGATCAAATCTCGCGCCTGACAGGCGTGGGGGCAGGGGCGTCACCTAACAATCTGCTGGATCAGCGCGATCAACTGGCGAGCGA                                                                           >  SRR4302540.3266457/1‑94 (MQ=60)
                     CAGCCTGAACGATCAAATCTCGCGCCTGACAGGCGTGGGGGCAGGGGCGTCACCTAACAATCTGCTGGATCAGCGCGATCAACTGGTGAGCGAA                                                                          <  SRR4302540.6738838/94‑1 (MQ=60)
                      AGCCTGAACGATCAAATCTCGCGCCTGACAGGCGTGGGGGCAGGGGCGTCACCTAACAATCTGCTGGATCAGCGCGATCAACTGGTGAGCGAAT                                                                         >  SRR4302540.9820259/1‑94 (MQ=60)
                        CCTGAACGATCAAATCTCGCGCCTGACAGGCGTGGGGGCAGGGGCGTCACCTAACAATCTGCTGGATCAGCGCGATCAACTGGTGAGCGAATTA                                                                       <  SRR4302540.713532/94‑1 (MQ=60)
                        CCTGAACGATCAAATCTCGCGCCTGACAGGCGTGGGGGCAGGGGCGTCACCTAACAATCTGCTGGATCAGCGCGATCAACTGGTGAGCGAATTA                                                                       <  SRR4302540.4057899/94‑1 (MQ=60)
                        CCTGAACGATCAAATCTCGCGCCTGACAGGCGTGGGGGCAGGGGCGTCACCTAACAATCTGCTGGATCAGCGCGATCAACTGGTGAGCGAATTA                                                                       >  SRR4302540.5229440/1‑94 (MQ=60)
                         CTGAACGATCAAATCTCGCGCCTGACAGGCGTGGGGGCAGGGGCGTCACCTAACAATCTGCTGGATCAGCGCGATCAACTGGTGAGCGAATTAA                                                                      >  SRR4302540.3646353/1‑94 (MQ=60)
                          TGAACGATCAAATCTCGCGCCTGACAGGCGTGGGGGCAGGGGCGTCACCTAACAATCTGCTGGATCAGCGCGATCAACTGGTGAGCGAATTAAA                                                                     >  SRR4302540.3071511/1‑94 (MQ=60)
                          TGAACGATCAAATCTCGCGCCTGACAGGCGTGGGGGCAGGGGCGTCACCTAACAATCTGCTGGATCAGCGCGATCAACTGGTGAGCGAATTAAA                                                                     >  SRR4302540.9111016/1‑94 (MQ=60)
                           GAACGATCAAATCTCGCGCCTGACAGGCGTGGGGGCAGGGGCGTCACCTAACAATCTGCTGGATCAGCGCGATCAACTGGTGAGCGAATTAAAC                                                                    >  SRR4302540.7984541/1‑94 (MQ=60)
                           GAACGATCAAATCTCGCGCCTGACAGGCGTGGGGGCAGGGGCGTCACCTAACAATCTGCTGGATCAGCGCGATCAACTGGTGAGCGAATTAAAC                                                                    >  SRR4302540.977648/1‑94 (MQ=60)
                            AACGATCAAATCTCGCGCCTGACAGGCGTGGGGGCAGGGGCGTCACCTAACAATCTGCTGGATCAGCGCGATCAACTGGTGAGCGAATTAAACC                                                                   <  SRR4302540.8014738/94‑1 (MQ=60)
                                ATCAAATCTCGCGCCTGACAGGCGTGGGGGCAGGGGCGTCACCTAACAATCTGCTGGATCAGCGCGATCAACTGGTGAGCGAATTAAACCAGAT                                                               >  SRR4302540.4647335/1‑94 (MQ=60)
                                 ACAAATCTCGCGCCTGACAGGCGTGGGGGCAGGGGCGTCACCTAACAATCTGCTGGATCAGCGCGATCAACTGGTGAGCGAATTAAACCAGATT                                                              <  SRR4302540.2281404/94‑1 (MQ=60)
                                    AATCTCGCGCCTGACAGGCGTGGGGGCAGGGGCGTCACCTAACAATCTGCTGGATCAGCGCGATCAACTGGTGAGCGAATTAAACCAGATTGTT                                                           >  SRR4302540.847441/1‑94 (MQ=60)
                                     ATCTCGCGCCTGACAGGCGTGGGGGCAGGGGCGTCACCTAACAATCTGCTGGATCAGCGCGATCAACTGGTGAGCGAATTAAACCAGATTGTTG                                                          <  SRR4302540.6576391/94‑1 (MQ=60)
                                     ATCTCGCGCCTGACAGGCGTGGGGGCAGGGGCGTCACCTAACAATCTGCTGGATCAGCGCGATCAACTGGTGAGCGAATTAAACCAGATTGTTG                                                          <  SRR4302540.3538274/94‑1 (MQ=60)
                                      TCTCGCGCCTGACAGGCGTGGGGGCAGGGGCGTCACCTAACAATCTGCTGGATCAGCGCGATCAACTGGTGAGCGAATTAAACCAGATTGTTGG                                                         <  SRR4302540.63156/94‑1 (MQ=60)
                                         CGCGCCTGACAGGCGTGGGGGCAGGGGCGTCACCTAACAATCTGCTGGATCAGCGCGATCAACTGGTGAGCGAATTAAACCAGATTGTTGGTGT                                                      <  SRR4302540.6997065/94‑1 (MQ=60)
                                           CGCCTGACAGGCGTGGGGGCAGGGGCGTCACCTAACAATCTGCTGTATCAGCGCGATCAACTGGTGAGCGAATTAAACCAGATTGTTGGTGTAG                                                    >  SRR4302540.8491721/1‑94 (MQ=60)
                                           CGCCTGACAGGCGTGGGGGCAGGGGCGTCACCTAACAATCTGCTGGATCAGCGCGATCAACTGGTGAGCGAATTAAACCAGATTGTTGGTGTAG                                                    >  SRR4302540.3342724/1‑94 (MQ=60)
                                           CGCCTGACAGGCGTGGGGGCAGGGGCGTCACCTAACAATCTGCGGGGTCAGCGCTATCAACTGGTGATCGAATTAAAGCAGATTGTTGtgggag                                                    >  SRR4302540.3342723/1‑88 (MQ=60)
                                            GCCTGACAGGCGTGGGGGCAGGGGCGTCACCTAACAATCTGCTGGATCAGCGCGATCAACTGGTGAGCGAATTAAACCAGATTGTTGGTGTAGA                                                   <  SRR4302540.76166/94‑1 (MQ=60)
                                             CGTGACAGGCGTGGGGGCAGGGGCGTCACCTAACAATCTGCTGGATCAGCGCGATCAACTGGTGAGCGAATTAAACCAGATTGTTGGTGTAGAA                                                  <  SRR4302540.3293173/94‑1 (MQ=60)
                                             CCTGACAGGCGTGGGGGCAGGGGCGTCACCTAACAATCTGCTGGATCAGCGCGATCAACTGGTGAGCGAATTAAACCAGATTGTTGGTGTGGAA                                                  <  SRR4302540.1859891/94‑1 (MQ=60)
                                             CCTGACAGGCGTGGGGGCAGGGGCGTCACCTAACAATCTGCTGGATCAGCGCGATCAACTGGTGAGCGAATTAAACCAGATTGTTGGTGTAGAC                                                  >  SRR4302540.6728940/1‑94 (MQ=60)
                                             CCTGACAGGCGTGGGGGCAGGGGCGTCACCTAACAATCTGCTGGATCAGCGCGATCAACTGGTGAGCGAATTAAACCAGATTGTTGGTGTAGAA                                                  <  SRR4302540.5150369/94‑1 (MQ=60)
                                             CCTGACAGGCGTGGGGGCAGGGGCGTCACCTAACAATCTGCTGGATCAGCGCGATCAACTGGTGAGCGAATTAAACCAGATTGTTGGTGTAGAA                                                  <  SRR4302540.5182584/94‑1 (MQ=60)
                                             CCTGACAGGCGTGGGGGCAGGGGCGTCACCTAACAATCTGCTGGATCAGCGCGATCAACTGGTGAGCGAATTAAACCAGATTGTTGGTGTAGAA                                                  <  SRR4302540.5235319/94‑1 (MQ=60)
                                             CCTGACAGGCGTGGGGGCAGGGGCGTCACCTAACAATCTGCTGGATCAGCGCGATCAACTGGTGAGCGAATTAAACCAGATTGTTGGTGTAGAA                                                  >  SRR4302540.6728939/1‑94 (MQ=60)
                                             CCTGACAGGCGTGGGGGCAGGGGCGTCACCTAACAATCTGCTGGATCAGCGCGATCAACTGGTGAGCGAATTAAACCAGATTGTTGGTGTAGAA                                                  >  SRR4302540.5301115/1‑94 (MQ=60)
                                              CTGACAGGCGTGGGGGCAGGGGCGTCACCTAACAATCTGCTGGATCAGCGCGATCAACTGGTGAGCGAATTAAACCAGATTGTTGGTGTAGAAG                                                 <  SRR4302540.7131074/94‑1 (MQ=60)
                                              CTGACAGGCGTGGGGGCAGGGGCGTCACCTAACAATCTGCTGGATCAGCGCGATCAACTGGTGAGCGAATTAAACCAGATTGTTGGTGTAGAAG                                                 >  SRR4302540.4413500/1‑94 (MQ=60)
                                              CTGACAGGCGTGGGGGCAGGGGCGTCACCTAACAATCTGCTGGATCAGCGCGATCAACTGGGGAGCGAATTAAACCAGATTGTTGGTGTAGAAG                                                 >  SRR4302540.834519/1‑94 (MQ=60)
                                               TGACAGGCGTGGGGGCAGGGGCGTCACCTAACAATCTGCTGGCTCAGCGCGATCAACTGGTGAGCGAATTAAACCAGATTGTTGGGGTAGAAGC                                                >  SRR4302540.3214336/1‑94 (MQ=60)
                                               TGACAGGCGTGGGGGCAGGGGCGTCACCTAACAATCTGCTGGATCAGCGCGATCAACTGGTGAGCGAATTAAACCAGATTGTTGGTGTAGAAGT                                                >  SRR4302540.524530/1‑94 (MQ=60)
                                               TGACAGGCGTGGGGGCAGGGGCGTCACCTAACAATCTGCTGGATCAGCGCGATCAACTGGTGAGCGAATTAAACCAGATTGTTGGTGTAGAAGT                                                >  SRR4302540.6545686/1‑94 (MQ=60)
                                               TGACAGGCGTGGGGGCAGGGGCGTCACCTAACAATCTGCTGGATCAGCGCGATCAACTGGTGAGCGAATTAAACCAGATTGTTGGTGTAGAAGT                                                >  SRR4302540.8105835/1‑94 (MQ=60)
                                               TGACAGGCGTGGGGGCAGGGGCGTCACCTAACAATCTGCTGGATCAGCGCGATCAACTGGTGAGCGAATTAAACCAGATTGTTGGTGTAGAAGC                                                >  SRR4302540.8354937/1‑94 (MQ=60)
                                                 ACAGGCGTGGGGGCAGGGGCGTCACCTAACAATCTGCTGGATCAGCGCGATCAACTGGTGAGCGAATTAAACCAGATTGTTGGTGTAGAAGTCA                                              <  SRR4302540.6616638/94‑1 (MQ=60)
                                                  CAGGCGTGGGGGCAGGGGCGTCACCTAACAATCTGCTGGATCAGCGCGATCAACTGGTGAGCGAATTAAACCAGATTGTTGGTGTAGAAGTCAG                                             <  SRR4302540.8394716/94‑1 (MQ=60)
                                                  AAGGCGTGGGGGCAGGGGCGTCACCTAACAATCTGCTGGATCAGCGCGATCAACTGGTGAGCGAATTAAACCAGATTGTTGGTGTAGAAGTCAG                                             <  SRR4302540.8394715/94‑1 (MQ=60)
                                                      CGTGGGGGCAGGGGCGTCACCTAACAATCTGCTGGATCAGCGCGATCAACTGGTGAGCGAATTAAACCAGATTGTTGGTGTAGAAGTCAGggtc                                         >  SRR4302540.723579/1‑90 (MQ=60)
                                                      CGTGGGGGCAGGGGCGTCACCTAACAATCTGCTGGATCAGCGCGATCAACTGGTGAGCGAATTAAACCAGATTGTTGGTGTAGAAGTCAGCGTT                                         <  SRR4302540.9753806/94‑1 (MQ=60)
                                                      CGTGGGGGCAGGGGCGTCACCTAACAATCTGCTGGATCAGCGCGATCAACTGGTGAGCGAATTAAACCAGATTGTTGGTGTAGAAGTCAGCGTT                                         <  SRR4302540.5459574/94‑1 (MQ=60)
                                                       GTGGGGGCAGGGGCGTCACCTAACAATCTGCTGGATCAGCGCGATCAACTGGTGAGCGAATTAAACCAGATTGTTGGTGTAGAAGTCAGCGTTC                                        <  SRR4302540.3364731/94‑1 (MQ=60)
                                                        TGGGGGCAGGGGCGTCACCTAACAATCTGCTGGATCAGCGCGATCAACTGGTGAGCGAATTAAACCAGATTGTTGGTGTAGAAGTCAGCGTTCA                                       <  SRR4302540.779958/94‑1 (MQ=60)
                                                        TGGGGGCAGGGGCGTCACCTAACAATCTGCTGGATCAGCGCGATCAACTGGTGAGCGAATTAAACCAGATTGTTGGTGTAGAAGTCAGCGTTCA                                       <  SRR4302540.3721690/94‑1 (MQ=60)
                                                         GGGGGCAGGGGCGTCACCTAACAATCTGCTGGATCAGCGCGATCAACTGGTGAGCGAATTAAACCAGATTGTTGGTGTAGAAGTCAGCGTTCAG                                      <  SRR4302540.2423546/94‑1 (MQ=60)
                                                         GGGGGCAGGGGCGTCACCTAACAATCTGCTGGATCAGCGCGATCAACTGGTGAGCGAATTAAACCAGATTGTTGGTGTAGAAGTCAGCGTTCAG                                      <  SRR4302540.3449763/94‑1 (MQ=60)
                                                         GGGGGCAGGGGCGTCACCTAACAATCTGCTGGATCAGCGCGATCAACTGGTGAGCGAATTAAACCAGATTGTTGGTGTAGAAGTCAGCGTTCAG                                      <  SRR4302540.3449762/94‑1 (MQ=60)
                                                          GGGGCAGGGGCGTCACCTAACAATCTGCTGGATCAGCGCGATCAACTGGTGAGCGAATTAAACCAGATTGTTGGTGTAGAAGTCAGCGTTCAGG                                     <  SRR4302540.7382868/94‑1 (MQ=60)
                                                           GGGCAGGGGCGTCACCTAACAATCTGCTGGATCAGCGCGATCAACTGGTGAGCGAATTAAACCAGATTGTTGGTGTAGAAGTCAGCGTTCAGGA                                    <  SRR4302540.8133852/94‑1 (MQ=60)
                                                            GGCAGGGGCGTCACCTAACAATCTGCTGGATCAGCGCGTTCAACTGGTGAGCGAATTAAACCAGATTGTTGGTGTAGAAGTCAGCGTTCAGGAT                                   <  SRR4302540.122221/94‑1 (MQ=60)
                                                            GGCAGGGGCGTCACCTAACAATCTGCTGGATCAGCGCGATCAACTGGTGAGCGAATTAAACCAGATTGTTGGTGTAGAAGTCAGCGTTCAGGAT                                   <  SRR4302540.9195832/94‑1 (MQ=60)
                                                            GGCAGGGGCGTCACCTAACAATCTGCTGGATCAGCGCGATCAACTGGTGAGCGAATTAAACCAGATTGTTGGTGTAGAAGTCAGCGTTCAGGAT                                   >  SRR4302540.8757717/1‑94 (MQ=60)
                                                            GGCAGGGGCGTCACCTAACAATCTGCTGGATCAGCGCGATCAACTGGTGAGCGAATTAAACCAGATTGTTGGTGTAGAAGTCAGCGTTCAGGAT                                   <  SRR4302540.5708675/94‑1 (MQ=60)
                                                             GCAGGGGCGTCACCTAACAATCTGCTGGATCAGCGCGATCAACTGGTGAGCGAATTAAACCAGATTGTTGGTGTAGAAGTCAGCGTTCAGGATG                                  <  SRR4302540.4825503/94‑1 (MQ=60)
                                                             GCAGGGGCGTCACCTAACAATCTGCTGGATCAGCGCGATCAACTGGTGAGCGAATTAAACCAGATTGTTGGTGTAGAAGTCAGCGTTCAGGATG                                  <  SRR4302540.4825504/94‑1 (MQ=60)
                                                              CAGGGGCGTCACCTAACAATCTGCTGGATCAGCGCGATCAACTGGTGAGCGAATTAAACCAGATTGTTGGTGTAGAAGTCAGCGTTCAGGATGG                                 >  SRR4302540.5832262/1‑94 (MQ=60)
                                                               AGGGGCGTCACCTAACAATCTGCTGGATCAGCGCGATCAACTGGTGAGCGAATTAAACCAGATTGTTGGTGTAGAAGTCAGCGTTCAGGATGGC                                >  SRR4302540.1846230/1‑94 (MQ=60)
                                                                GGGGCGTCACCTAACAATCTGCTGGATCAGCGCGATCAACTGGTGAGCGAATTAAACCAGATTGTTGGTGTAGAAGTCAGGGTTCAGGATGGCG                               >  SRR4302540.6953320/1‑94 (MQ=60)
                                                                GGGGCGTCACCTAACAATCTGCTGGATCAGCGCGATCAACTGGTGAGCGAATTAAACCAGATTGTTGGTGTAGAAGTCAGCGTTCAGGATGGCG                               >  SRR4302540.7318954/1‑94 (MQ=60)
                                                                  GGCGTCACCTAACAATCTGCTGGATCAGCGCGATCAACTGGTGAGCGAATTAAACCAGATTGTTGGTGTAGAAGTCAGCGTTCAGGATGGCGGC                             >  SRR4302540.983936/1‑94 (MQ=60)
                                                                  GACGTCACCTAACAAGCTGCTGGATCAGCGCGATCAACTGGTGAGCGAATTAAACCAGATTGTTGGTGTAGAAGTCAGCGTTCAGGATGGCGGC                             <  SRR4302540.7511321/94‑1 (MQ=60)
                                                                   GCGTCACCTAACAATCTGCTGGATCAGCGCGATCAACTGGTGAGCGAATTAAACCAGATTGTTGGTGTAGAAGTCAGCGTTCAGGATGGCGGCA                            >  SRR4302540.421623/1‑94 (MQ=60)
                                                                   GCGTCACCTAACAATCTGCTGGATCAGCGCGATCAACTGGTGAGCGAATTAAACCAGATTGTTGGTGTAGAAGTCAGCGTTCAGGATGGCGGCA                            <  SRR4302540.6782052/94‑1 (MQ=60)
                                                                    CGTCACCTAACAATCTGCTGGATCAGCGCGATCAACTGGTGAGCGAATTAAACCAGATTGTTGGTGTAGAAGTCAGCGTTCAGGATGGCGGCAC                           <  SRR4302540.7813608/94‑1 (MQ=60)
                                                                    CGTCACCTAACAATCTGCTGGATCAGCGCGATCAACTGGTGAGCGAATTAAACCAGATTGTTGGTGTAGAAGTCAGCGTTCAGGATGGCGGCAC                           >  SRR4302540.3065601/1‑94 (MQ=60)
                                                                     GTCACCTAACAATCTGCTGGATCAGCGCGATCAACTGGTGAGCGAATTAAACCAGATTGTTGGTGTAGAAGTCAGCGTTCAGGATGGCGGCACT                          >  SRR4302540.3846835/1‑94 (MQ=60)
                                                                      TCACCTAACAATCTGCTGGATCAGCGCGATCAACTGGTGAGCGAATTAAACCAGATTGTTGGTGTAGAAGTCAGCGTTCAGGATGGCGGCACTT                         >  SRR4302540.4840675/1‑94 (MQ=60)
                                                                        ACCTAACAATCTGCTGGATCAGCGCGATCAACTGGTGAGCGAATTAAACCAGATTGTTGGTGTAGAAGTCAGCGTTCAGGATGGCGGCACTTAT                       <  SRR4302540.4788349/94‑1 (MQ=60)
                                                                         CCTAACAATCTGCTGGATCAGCGCGATCAACTGGTGAGCGAATTAAACCAGATTGTTGGTGTAGAAGTCAGCGTTCAGGATGGCGGCACTTATA                      <  SRR4302540.8804740/94‑1 (MQ=60)
                                                                          CTAACAATCTGCTGGATCAGCGCGATCAACTGGTGAGCGAATTAAACCAGATTGTTGGTGTAGAAGTCAGCGTTCAGGATGGCGGCACTTATAA                     <  SRR4302540.3687540/94‑1 (MQ=60)
                                                                           TAACAATCTGCTGGATCAGCGCGATCAACTGGTGAGCGAATTAAACCAGATTGTTGGTGTAGAAGTCAGCGTTCAGGATGGCGGCACTTATAAC                    <  SRR4302540.2371787/94‑1 (MQ=60)
                                                                            AACAATCTGCTGGATCAGCGCGATCAACTGGTGAGCGAATTAAACCAGATTGTTGGTGTAGAAGTCAGCGTTCAGGATGGCGGCACTTATAACA                   >  SRR4302540.2471459/1‑94 (MQ=60)
                                                                            AACAATCTGCTGGATCAGCGCGATCAACTGGTGAGCGAATTAAACCAGATTGTTGGTGTAGAAGTCAGCGTTCAGGATGGCGGCACTTATAACA                   <  SRR4302540.873640/94‑1 (MQ=60)
                                                                             ACAATCTGCTGGATCAGCGCGATCAACTGGTGAGCGAATTAAACCAGATTGTTGGTGTAGAAGTCAGCGTTCAGGATGGCGGCACTTATAACAT                  <  SRR4302540.7193842/94‑1 (MQ=60)
                                                                             ACAATCTGCTGGATCAGCGCGATCAACTGGTGAGCGAATTAAACCAGATTGTTGGTGTAGAAGTCAGCGTTCAGGATGGCGGCACTTATAACAT                  <  SRR4302540.807609/94‑1 (MQ=60)
                                                                             ACAATCTGCTGGATCAGCGCGATCAACTGGTGAGCGAATTAAACCAGATTGTTGGTGTAGAAGTCAGCGTTCAGGATGGCGGCACTTATAACAT                  <  SRR4302540.1047360/94‑1 (MQ=60)
                                                                              CAATCTGCTGGATCAGCGCGATCAACTGGTGAGCGAATTAAACCAGATTGTTGGTGTAGAAGTCAGCGTTCAGGATGGCGGCACTTATAACATC                 <  SRR4302540.3885936/94‑1 (MQ=60)
                                                                              CAATCTGCTGGATCAGCGCGATCAACTGGTGAGCGAATTAAACCAGATTGTTGGTGTAGAAGTCAGCGTTCAGGATGGCGGCACTTATAACATC                 <  SRR4302540.2127920/94‑1 (MQ=60)
                                                                               AATCTGCTGGATCAGCGCGATCAACTGGTGAGCGAATTAAACCAGATTGTTGGTGTAGAAGTCAGCGTTCAGGATGGCGGCACTTATAACATCA                >  SRR4302540.8608199/1‑94 (MQ=60)
                                                                                ATCTGCTGGATCAGCGCGATCAACTGGTGAGCGAATTAAACCAGATTGTTGGTGTAGAAGTCAGCGTTCAGGATGGCGGCACTTATAACATCAC               <  SRR4302540.2596667/94‑1 (MQ=60)
                                                                                ATCTGCTGGATCAGCGCGATCAACTGGTGAGCGAATTAAACCAGATTGTTGGTGTAGAAGTCAGCGTTCAGGATGGCGGCACTTATAACATCAC               <  SRR4302540.2604396/94‑1 (MQ=60)
                                                                                 TCTGCTGGATCAGCGCGATCAACTGGTGAGCGAATTAAACCAGATTGTTGGTGTAGAAGTCAGCGTTCAGGATGGCGGCACTTATAACATCACG              >  SRR4302540.1972559/1‑94 (MQ=60)
                                                                                  CTGCTGGATCAGCGCGATCAACTGGTGAGCGAATTAAACCAGATTGTTGGTGTAGAAGTCAGCGTTCAGGATGGCGGCACTTATAACATCACGA             <  SRR4302540.9938228/94‑1 (MQ=60)
                                                                                   TGCTGGATCAGCGCGATCAACTGGTGAGCGAATTAAACCAGATTGTTGGTGTAGAAGTCAGCGTTCAGGATGGCGGCACTTATAACATCACGAT            >  SRR4302540.8916771/1‑94 (MQ=60)
                                                                                   TGCTGGATCAGCGCGATCAACTGGTGAGCGAATTAAACCAGATTGTTGGTGTAGAAGTCAGCGTTCAGGATGGCGGCACTTATAACATCACGAT            <  SRR4302540.7810825/94‑1 (MQ=60)
                                                                                     CTGGATCAGCGCGATCAACTGGTGAGCGAATTAAACCAGATTGTTGGTGTAGAAGTCAGCGTTCAGGATGGCGGCACTTATAACATCACGATGG          >  SRR4302540.9701441/1‑94 (MQ=60)
                                                                                     CTGGATCAGCGCGATCAACTGGTGAGCGAATTAAACCAGATTGTTGGTGTAGAAGTCAGCGTTCAGGATGGCGGCACTTATAACATCACGATGG          <  SRR4302540.6570838/94‑1 (MQ=60)
                                                                                      TGGATCAGCGCGATCAACTGGTGAGCGAATTAAACCAGATTGTTGGTGTAGAAGTCAGCGTTCAGGATGGCGGCACTTATAACATCACGATGGC         >  SRR4302540.3602618/1‑94 (MQ=60)
                                                                                       GGATCAGCGCGATCAACTGGTGAGCGAATTAAACCAGATTGTTGGTGTAGAAGTCAGCGTTCAGGATGGCGGCACTTATAACATCACGATGGCC        >  SRR4302540.3644559/1‑94 (MQ=60)
                                                                                       GGATCAGCGCGATCAACTGGTGAGCGAATTAAACCAGATTGTTGGTGTAGAAGTCAGCGTTCAGACTGGCGGCACTTATAACATCACGATGGCC        >  SRR4302540.4624686/1‑94 (MQ=60)
                                                                                        GATCAGCGCGATCAACTGGTGAGCGAATTAAACCAGATTGTTGGTGTAGAAGTCAGCGTTCAGGATGGCGGCACTTATAACATCACGATGGCCA       >  SRR4302540.6382168/1‑94 (MQ=60)
                                                                                          TCAGCGCGATCAACTGGTGAGCGAATTAAACCAGATTGTTGGTGTAGAAGTCAGCGTTCAGGATGGCGGCACTTATAACATCACGATGGCCAAT     <  SRR4302540.5785926/94‑1 (MQ=60)
                                                                                           CAGCGCGATCAACTGGTGAGCGAATTAAACCAGATTGTTGGTGTAGAAGTCAGCGTTCAGGATGGCGGCACTTATAACATCACGATGGCCAATG    >  SRR4302540.4472858/1‑94 (MQ=60)
                                                                                             GCGCGATCAACTGGTGAGCGAATTAAACCAGATTGTTGGTGTAGAAGTCAGCGTTCAGGATGGCGGCACTTATAACATCACGATGGCCAATGGT  <  SRR4302540.4469964/94‑1 (MQ=60)
                                                                                             |                                                                                             
CAACTACGCTAAACAAATTGCCAGCCTGAACGATCAAATCTCGCGCCTGACAGGCGTGGGGGCAGGGGCGTCACCTAACAATCTGCTGGATCAACGCGATCAACTGGTGAGCGAATTAAACCAGATTGTTGGTGTAGAAGTCAGCGTTCAGGATGGCGGCACTTATAACATCACGATGGCCAATGGT  >  NC_000913/1138110‑1138296

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑